| studies on tyzzer's disease: a long-term study of the humoral antibody response in mice, rats and rabbits. | mice treated with an antigen prepared from livers infected with bacillus piliformis developed antibodies to the microorganism which reached a peak on the 7th day and disappeared within 40 days: antibody titres in experimentally-infected mice remained at a high level throughout life. the antibody titres in naturally-infected mice, rats and rabbits ramined positive throughout life and followed the same pattern as that of the experimentally-infected mice. | 1979 | 374860 |
| studies on tyzzer's disease: transplacental transmission of bacillus piliformis in rats. | clinically healthy rats with antibodies to bacillus piliformis were given prednisolone in the last week of pregnancy. b. piliformis was demonstrated in the livers of their offspring. none of the dams or the young rats showed clinical signs of disease. antibodies to b. piliformis were found in the young rats at birth, and presisted for several months. the importance of potential transplacental infections when attempting to establish colonies free from b. piliformis in discussed. | 1979 | 374861 |
| studies on tyzzer's disease: acquired immunity against infection and activation of infection by immunosuppressive treatment. | the correlation between serum antibody titre and resistance to challenge infection with bacillus piliformis was studied in naturally infected mice, in experimentally infected but recovered mice, and in mice treated with antigen prepared from infected livers. irrespective of the way in which the antibodies were acquired resistance to infection was found to be related to the immunofluorescence antibody titre found. experimentally infected but recovered mice, as well as rats with persistent antibod ... | 1979 | 384085 |
| the influence of bacillus piliformis (tyzzer) infections on the reliability of pharmacokinetic experiments in mice. | the half-lives of warfarin and trimethoprim were significantly longer in mice acutely infected with bacillus piloformis and in mice which ad clinically recovered from previous experimental infection with the organism. the volume of distribution of trimethoprim but not of warfarin was significantly greater in infected mice than in controls. body clearances of warfarin was significantly reduced in both disease states. for trimethoprim this parameter was only reduced in the acute state of the disea ... | 1979 | 553198 |
| naturally occurring tyzzer's disease and intestinal spirochetosis in guinea pigs. | bacillus piliformis infection (tyzzer's disease) occurred in two young guinea pigs, causing unthriftiness and diarrhea which resulted in death. there was necrosis and inflammation of the ileum, cecum, and colon. intestinal epithelial cells contained organisms resembling bacillus piliformis. spirochetes were found in the cecum and colon, mainly in crypts. acute diarrhea occurred in another guinea pig which became cachetic and was killed. histologically, large numbers of spirochetes were present i ... | 1978 | 642440 |
| naturally occurring tyzzer's disease in the gray fox. | four gray fox became anorectic and developed diarrhea. their condition worsened until death. diagnosis of tyzzer's disease was made on the basis of clinical signs, gross and microscopic lesions, and the demonstration of bacillus piliformis in the liver. | 1978 | 738940 |
| isolation of bacillus piliformis from rabbits. | | 1977 | 849582 |
| tyzzer's disease in wild-trapped muskrats in british columbia. | a diagnosis of tyzzer's disease (bacillus piliformis infection) was made in a group of recently-trapped muskrats (ondatra zibethica). the major lesions consisted of enteritis and focal necrosis of the liver, with organisms resembling b. piliformis in hepatocytes on the periphery of these foci. the outbreak was associated with recent capture, housing, and the use of sulfaquinoxaline-medicated feed. | 1977 | 864842 |
| studies on tyzzer's disease: isolation and propagation of bacillus piliformia. | mice from a colony with subclinical tyzzer's disease were treated with prednisolone in order to activate a bacillus piliformis infection. from the livers of these mice the organism was isolated and serially passaged in embryonated eggs. it resisted heating at 75 degrees c for 20 minutes and was still infective after storage at -80 degree c for 24 days, but not for 52 days or more. infectively was maintained for more than a year by storing infectious liver tissue at -190 degrees c. b. pilifor ... | 1977 | 865074 |
| tyzzer's disease in syrian hamsters. | tyzzer's disease was diagnosed in 18 syrian hamsters. the clinical signs included sudden onset of diarrhea, dehydration, and lethargy; all affected hamsters died within 48 hours. gross lesions consisted of multiple white nodules in the heart; dilated cecum and colon containing semiliquid feces, sometimes bearing necrotic plaques on the mucosa; and occasionally, a few small scattered white spots in the liver. microscopic study of those lesions revealed areas of necrosis and mixed cellular inflamm ... | 1977 | 924852 |
| naturally occurring tyzzer's disease as a complication of distemper and mycotic pneumonia in a dog. | tyzzer's disease, distemper, and mycotic pneumonia were diagnosed at necropsy of a 6-week-old mixed breed pup. the illness was characterized by lethargy, poor appetite, dysentery, and convulsions, the latter developing just prior to death. the necropsy findings included pale, dilated large intestine and swollen, dark liver. histologically, multifocal hepatic necrosis, similar to that of tyzzer's disease in other species of animals, was observed. bacillus piliformis was found in hepatocytes and n ... | 1976 | 956024 |
| naturally-occurring tyzzer's disease (bacillus piliformis infection) in horse foals. | spontaneous tyzzer's disease is described in quarter horse foals which died suddenly with no clinical history of apparent illness. significant gross findings included icterus, focal paletan areas in the liver and catarrhal entercolitis. focal dark red lesions were present in the small intestine of one foal, and the mesenteric lymph nodes of another were enlarged and hyperemic. histopathologically, the liver showed multiple discrete and confluent foci of necrosis, fatty change, sinusoid congestio ... | 1975 | 1119056 |
| tyzzer's disease in a dog. | a 5-week-old mixed-breed dog was examined because of emaciation and depression associated with chronic anorexia, diarrhea, and vomiting. its rectal temperature was subnormal and it died on the day of admission. at necropsy, small focal lesions were distributed through the liver. enteric alterations included catarrhal enteritis with fluid contents, excess production of mucus, and mucosal hyperemia. microscopically, the hepatic lesions were disseminated foci of coagulative necrosis, with little or ... | 1976 | 1270343 |
| subclinical infection and transmission of tyzzer's disease in rats. | two isolates of bacillus piliformis originally obtained from rats from japan and indiana were compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and western blotting. protein and antigen profiles revealed heterogeneity between the two isolates, demonstrating that more than one isolate of b. piliformis is capable of infecting rats. results of parallel infection and transmission studies with the two isolates were almost identical. orally inoculated rats remained asymptomatic; ho ... | 1992 | 1460840 |
| rederivation of rat colonies seropositive for bacillus piliformis and the subsequent screening for antibodies. | latent infection of rats in a breeding colony with bacillus piliformis detectable by antibodies to the agent in an immunofluorescence assay was eliminated by a combination of traditional rederivation techniques, using animal units not previously used for rat breeding, and the use of specific disinfection procedures. the success rate was apparently correlated with the use of peracetic acid instead of aldehyde products to decontaminate the animal unit. | 1992 | 1460841 |
| correlation between megaloileitis and antibodies to bacillus piliformis in laboratory rat colonies. | rat colonies in which antibodies to bacillus piliformis were detected in animals examined at the age of 8 to 15 weeks were compared with rat colonies where no such antibodies were present. the seropositive colonies had a low incidence of megaloileitis in 5-week-old rats of sprague-dawley stock and some few inbred strains. in seronegative colonies, no megaloileitis was detected. in rats with megaloileitis, significantly high titers to b. piliformis were noted and the agents could be identified in ... | 1992 | 1460842 |
| bacillus piliformis flagellar antigens for serodiagnosis of tyzzer's disease. | purified flagella from multiple isolates of bacillus piliformis were obtained and examined by electron microscopy. sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and western blot (immunoblot) analyses were used to assess the purity, antigenicity, and cross-reactivity of purified flagellar preparations. sds-page demonstrated a single, major protein band evident at approximately 53 to 56 kda in all isolates tested. results of western blot analyses indicated a lack of cross-re ... | 1991 | 1774263 |
| tyzzer's disease (bacillus piliformis) in australian marsupials. | tyzzer's disease (bacillus piliformis infection) was diagnosed in nine marsupials (six possums, a koala, a wombat and a dasyurid). all but two of the possums were captive. five of the seven marsupials, for which ages were recorded, were juvenile. affected marsupials were either found dead or were showing non-specific illness for up to two days before death. affected livers and hearts showed gross haemorrhage and scattered areas of white discolouration. microscopically, areas of coagulative necro ... | 1991 | 1779038 |
| detection of serum antibodies to bacillus piliformis in mice and rats using an enzyme-linked immunosorbent assay. | rats and mice were infected with bacillus piliformis organisms at a dosage which resulted in clinical signs of tyzzer's disease in gerbils. although rats and mice did not show clinical signs of disease, rising antibody titers to b. piliformis were detected by enzyme-linked immunosorbent assay (elisa) 2 to 6 weeks post-inoculation and remained at positive levels 11 weeks post-inoculation. western blot analyses of sera from experimentally infected animals revealed banding patterns nearly identical ... | 1991 | 1849582 |
| tyzzer disease in hamsters and gerbils from a pet store supplier. | an episode of tyzzer disease (bacillus piliformis) developed in hamster and gerbil colonies of a pet store supplier. the incidence of diarrhea and subsequent mortality was high. the only important necropsy findings were cecal distention and mesenteric lymphadenopathy in the hamsters. histologically, necrotizing typhlitis and hepatitis with associated b piliformis organisms were seen in both species. this case was unusual because the most consistent gross lesion associated with tyzzer disease--he ... | 1990 | 2254145 |
| seromonitoring in small laboratory animal colonies. a five year survey: 1984-1988. | from 1984 to 1988 one thousand serologic investigations of laboratory animal colonies originating from 10 different european countries were performed. the most prevalent infections in mouse stocks were caused by mouse hepatitis virus (mhv), minute virus of mice (mvm), theiler's encephalomyelitis virus (temv), reovirus type 3 (reo3), sendaivirus, and pneumonia virus of mice (pvm). in mice no infections with lymphocytic choriomeningitis virus (lcm), polyomavirus, mouse adenovirus, and k-virus were ... | 1990 | 2321435 |
| cultivation of bacillus piliformis (tyzzer) in mouse fibroblasts (3t3 cells). | bacillus piliformis was successfully propagated in a continuous mouse-embryo fibroblast cell line (3t3). 3t3 monolayers were successfully inoculated with a variety of infected materials including yolk sac and liver suspensions, minced yolk sac and liver, and primary chicken-embryo liver-cell cultures. an initial decrease in b. piliformis number was noted after inoculation of the cell layer with 2.6 x 10(5) organisms followed by a peak bacterial count at 48 h. bacillus piliformis remained infecti ... | 1990 | 2353449 |
| enterohepatitis in mongolian gerbils (meriones unguiculatus) inoculated perorally with tyzzer's organism (bacillus piliformis). | enterohepatitis was produced in mongolian gerbils by intragastric inoculation with tyzzer's organism from natural infection of a gerbil. death occurred in 50 to 60% animals 5 to 7 days postinoculation (p.i.). on day 3 p.i., when a few necrotic foci appeared in the liver, a large amount of bacterial antigen was present within ileocecal enterocytes and reticuloendothelial cells of the peyer's patches. neutrophil and monocyte infiltration was found in the lamina propria. on day 5 or 6 p.i. there wa ... | 1989 | 2918680 |
| bacillus piliformis infection in an adult dog. | bacillus piliformis infection (tyzzer's disease) was diagnosed in a 7-year-old spayed dog that had icterus, hepatosplenomegaly, and polyuria. hematology revealed regenerative anemia, leukocytosis, lymphopenia, and thrombocytopenia. serum chemical analyses indicated hypocalcemia, high alkaline phosphatase activity, hypoalbuminemia, and hyperglobulinemia. at necropsy, the liver was stippled with gray-white focal lesions. microscopically, the liver lesions were necrotic and inflammatory. warthin-st ... | 1988 | 3356597 |
| serum antibody prevalence for herpesvirus sylvilagus, bacillus piliformis and california serogroup arboviruses in cottontail rabbits from pennsylvania. | a serologic survey of 60 eastern cottontail rabbits (sylvilagus floridanus) from three counties in pennsylvania was conducted in march 1983. serum antibody prevalences for herpesvirus sylvilagus and la crosse virus (california serogroup) were less than 4%. there was no evidence of previous exposure to either jamestown canyon or snowshoe hare viruses (california serogroup). antibody to trivittatus virus (california serogroup) was found in 60% of the 20 cottontails from york county. no cottontails ... | 1988 | 3373643 |
| [tyzzer's disease]. | tyzzer's disease occurs as an epizootic disease in a number of mammals including rabbits and is characterized by sever diarrhoea and high mortality. lesions consist of necrotic and hemorrhagic enteritis and are observed mainly in the distal digestive tract (ileum-caecum-colon). congestion and vivid reddening of the caecum and marked serosal and submucosal oedema are present. typical hepatic lesions, i.e. necrotic and miliary foci of the liver are generally absent in acute phase of the disease in ... | 1986 | 3548559 |
| significance of clostridium spiroforme in the enteritis-complex of commercial rabbits. | commercial rabbits showing clinical signs of enteritis-complex were examined for the presence of clostridium spiroforme and its iota-like toxin. the bacterium was detected by gram stain in 52.4% of 149 cecal samples and iota-like toxin in 7.4%. from 29 strains of c. spiroforme tested, 26 were toxigenic, originating from 24 of 29 rabbitries. in 13.4% of the samples, c. spiroforme was present as the only known disease agent. gross and microscopic lesions were similar to those described in the lite ... | 1986 | 3727365 |
| protein and antigenic heterogeneity among isolates of bacillus piliformis. | protein and antigenic heterogeneity among isolates of bacillus piliformis, the etiologic agent of tyzzer's disease, were investigated. the seven isolates utilized in this study were originally isolated from naturally infected animals of different animal species and diverse geographical locations. isolates were propagated in mammalian cell lines, and bacterial extracts were prepared. protein and antigenic profiles were compared among isolates, using coomassie blue-stained polyacrylamide gels and ... | 1990 | 2318527 |
| epidemiological studies of bacillus piliformis infection and tyzzer's disease in laboratory rats. | characteristics in connection with the presence of bacillus piliformis in the barrier-protected animal rooms of a breeding centre for laboratory rats are discussed. the incidence of antibodies against b. piliformis in ifa test is high -77.8% for the total breeding centre. clinical and pathological changes are, however, rare and mainly connected with certain rat strains. immunosuppression with prednisolone and subsequent histopathological tests of liver sections indicate that rats with mhc-type r ... | 1990 | 2146823 |
| suspected tyzzer's disease in two foals. | tyzzer's disease was diagnosed histologically in two foals, a 4-week-old thoroughbred cross and a 6-week-old arabian foal. clinically both foals were in good health prior to a short illness lasting only a few hours. the liver lesions in both foals were characterized microscopically by multiple foci of necrosis. variable numbers of elongated slender intracytoplasmic bacilli resembling bacillus piliformis were demonstrated within hepatocytes bordering the necrotic foci. | 1985 | 4020811 |
| colitis due to bacillus piliformis in two kittens. | | 1985 | 4082395 |
| application of immunofluorescence to detection of tyzzer's disease agent (bacillus piliformis) in experimentally infected mice. | | 1972 | 4553995 |
| a serological survey on bacillus piliformis infection in laboratory rabbits in japan. | a total of 544 rabbit sera obtained from 6 commercial breeding facilities and 9 research institutions during 1985-1990 were tested for bacillus piliformis antibody by an enzyme-linked immunosorbent assay (elisa) and indirect immunofluorescent antibody test (ifat). the antibody was detected in 53 (14.2%) rabbits from 3 breeding facilities and 30 (17.4%) rabbits from 6 research institutions, indicating the prevalence of b. piliformis infection among laboratory rabbits in japan. the overall agreeme ... | 1991 | 2044670 |
| electron microscopic studies of three forms of bacillus piliformis. | | 1983 | 6305061 |
| bacillus piliformis infection (tyzzer's disease) in a colorado foal. | | 1984 | 6469834 |
| cytotoxicity of bacillus piliformis. | seven isolates of b. piliformis, the agent of tyzzer's disease, obtained from various host species, were examined for cytotoxic activity by incubating culture filtrates on brl 3a rat-hepatocyte and 3t3 mouse-fibroblast cell lines. one isolate exhibited cytopathic effects on brl 3a cells, but not on 3t3 cells. three other isolates were strongly cytotoxic for 3t3 cells but only slightly so for brl 3a cells. the remaining three isolates showed no cytotoxicity for either cell line. the cytotoxic pro ... | 1992 | 1625320 |
| encephalitis in gerbils due to naturally occurring infection with bacillus piliformis (tyzzer's disease). | | 1992 | 1460855 |
| bacillus piliformis infection (tyzzer's disease) in two foals. | | 1976 | 1245447 |
| four cases of tyzzer's disease in foals in england. | a rodent pathogen, bacillus piliformis, has been recognised as causing a rapidly fatal hepatitis in 4 foals in england. the disease in foals has been recognised in america since 1973. a clinico-pathological account of the 4 cases is given and the differential diagnosis discussed. the 4 foals' ages fell within a very narrow range (24-34 days). some of the properties of this unusual intracellular pathogen are reviewed. for the first time in the equine the bacillus was seen in association with myoc ... | 1976 | 954720 |
| studies on tyzzer's disease: application of immunofluorescence for detection of bacillus piliformis and for demonstration and determination of antibodies to it in sera from mice and rabbits. | bacillus piliformis antigens were demonstrated in smear preparations from infected mouse livers by direct immunofluorescence technique. mouse serum antibodies against b. piliformis were demonstrated by indirect immunofluorescence technique. the test was employed quantitatively both on sera from experimentally infected mice and on sera from clinically healthy mice from colonies infected with b. piliformis, and could be used for the quantitative demonstration of antibodies in sera from a stock of ... | 1977 | 325290 |
| effect of heat and selected chemical disinfectants upon infectivity of spores of bacillus piliformis (tyzzer's disease). | spores of bacillus piliformis of rabbit origin were harvested from the yolk sac of previously inoculated hen's eggs and subjected to various heat or chemical disinfectant treatments. subsequently, spores were tested for infectivity in embryonated eggs inoculated via the yolk sac route, and others were treated with trypsin and then tested in embryonated eggs. the spores were not affected by the heat treatment of 60 degrees c but were rendered noninfective with treatments of 70 degrees c and 80 de ... | 1980 | 7052374 |
| demonstration of antibodies to bacillus piliformis in spf colonies and experimental transplacental infection by bacillus piliformis in mice. | antibodies to bacillus piliformis were demonstrated by the immunofluorescence antibody technique in sera from mice and rabbits from spf breeding colonies. mice in various stages of pregnancy were experimentally infected with bacillus piliformis and killed 2 to 3 days later. the organism was demonstrated in the uterus, foetal membranes and in the liver of the foetuses. infection was not limited to any particular stage of pregnancy. | 1978 | 146762 |
| studies on tyzzer's disease in rats. | an outbreak of an epidemic disease occurred in a specified-pathogen-free (spf) breeding colony of rats. the clinical signs and the post-mortem findings were characteristic for tyzzer's disease. the causative agent, bacillus piliformis, was demonstrated microscopically in ileum, liver and myocardium, and transmitted to mice where its pathogenicity appeared to be similar to that of another strain isolated from mice. b. piliformis from spontaneously-infected rats was demonstrated by indirect immuno ... | 1978 | 146761 |
| tyzzer's disease as a complication of canine distemper in a raccoon. | intercurrent canine distemper and tyzzer's disease were diagnosed in a mature raccoon (procyon lotor) submitted for necropsy. clinical, gross and microscopic findings characteristic of canine distemper virus (cdv), included ataxia, dyspnea, suppurative conjunctivitis, interstitial pneumonitis and generalized lymphocytolysis. inclusion bodies typical of cdv infection were present in many epithelial tissues. acute multifocal hepatic necrosis and acute segmental necrotizing enteritis were attribute ... | 1986 | 3951062 |
| antigenic relatedness of "bacillus piliformis" from tyzzer's disease occurring in japan and other regions. | | 1985 | 3920425 |
| application of enzyme-linked immunosorbent assay (elisa) to detection of antibodies against tyzzer's organism (bacillus piliformis) in mice. | | 1986 | 3820923 |
| assignment of the agent of tyzzer's disease to clostridium piliforme comb. nov. on the basis of 16s rrna sequence analysis. | the small-subunit rrna (16s rrna) sequence of tyzzer's bacillus (also known as "bacillus piliformis") was elucidated by using the polymerase chain reaction followed by reverse transcriptase sequencing. by using maximum-likelihood analysis, a phylogenetic tree was constructed from this and other 16s rrna sequences available from the first release of the ribosomal database project (g. j. olsen, r. overbeek, n. larsen, t. l. marsh, m. j. mccaughey, m. a. maciukenas, w.-m. kuan, t. j. macke, y. xing ... | 1993 | 7684241 |
| naturally occurring tyzzer's disease in a puppy. | a naturally occurring case of tyzzer's disease due to infection with bacillus piliformis in a wolf-dog hybrid resulted in widely disseminated lesions, including severe myocarditis, hepatitis, enterocolitis, intestinal leiomyositis, and adrenal cortical adenitis. previously reported lesions for canine tyzzer's disease have been limited to hepatic necrosis and a necrotizing enterocolitis. | 1995 | 7725599 |
| naturally occurring bacillus piliformis infection (tyzzer's disease) in guinea pigs. | two juvenile male hartley guinea pigs (cavia porcellus) were found dead 36 hours after receipt from a commercial source. both guinea pigs had dependent, subcutaneous edema and excess serous fluid in their thoracic and abdominal cavities. their livers were mottled and the cecal walls were reddened focally. histopathologic exam revealed nests of bacillus piliformis within the absorptive epithelial cells of the ileum, cecum and colon. vegetative organisms and spore-like structures were observed in ... | 1986 | 3773462 |
| experimentally induced tyzzer's disease in the african white-tailed rat (mystromys albicaudatus). | tyzzer's disease was induced experimentally in nonimmunosuppressed, weanling mystromys albicaudatus by oral inoculation with bacillus piliformis spores. focal areas of necrosis bordered by cells containing b. piliformis were observed first in the tunica muscularis of the intestine and in the periportal region of the liver 3 days post-inoculation, in the ventricular myocardium 7 days post-inoculation and in the brainstem 9 days post-inoculation. healing in the tunica muscularis, liver and myocard ... | 1986 | 3773459 |
| tyzzer's infection: host specificity of clostridium piliforme isolates. | tyzzer's disease, a well-recognized syndrome in numerous laboratory animal species, is caused by the obligate intracellular bacterium, clostridium piliforme. distinct isolates of c. piliforme from various laboratory animal species have been identified based on protein and antigenic heterogeneity. the goal of this study was to examine the host specificity of three well-characterized isolates of c. piliforme. groups of mice, rats, and hamsters were experimentally infected with isolates obtained fr ... | 1994 | 7898029 |
| electron microscopic observations on sporulation of tyzzer's organism, "bacillus piliformis". | | 1986 | 3735891 |
| inactivation of bacillus piliformis spores by heat and certain chemical disinfectants. | the inactivation of tyzzer's organism (bacillus piliformis) spore isolated from rats by heat and various chemical disinfectants was studied. the spores were from b. piliformis-infected rat liver tissues. the spore suspension (10(4) 50% of rat liver lesion producing dose with prednisolone treatment/ml) was treated with heart or disinfectants. inactivation of the spores was examined in experimentally infected rats. rats were inoculated perorally with a treated spore suspension and injected subcuta ... | 1987 | 3678367 |
| tyzzer's disease in a neonatal cockatiel. | a 4-day-old cockatiel that died suddenly had a pale, mottled liver at necropsy. extensive necrosis and numerous bacilli were present microscopically in the liver. the organism was identified as bacillus piliformis, the causative agent of tyzzer's disease, by special staining and electron microscopy. this is the first report of tyzzer's disease in an avian species. | 1993 | 8257388 |
| bacillus piliformis infection (tyzzer's disease) in a calf. | a 1-week-old jersey bull calf with a history of diarrhea, weakness, and lethargy was submitted for necropsy. principal macroscopic findings were enteritis and multifocal necrotizing hepatitis. histologically and ultrastructurally, organisms with characteristics of bacillus piliformis were associated with the foci of necrosis in the liver. | 1987 | 3654316 |
| an enzyme-linked immunosorbent assay for detection of anti-bacillus piliformis serum antibody in rabbits. | an enzyme-linked immunosorbent assay (elisa) is described for the detection of rabbit serum antibody directed against the causative agent of tyzzer's disease, bacillus piliformis. ninety-four percent agreement was found between the elisa and an indirect fluorescent antibody test. the sensitivity of the elisa was 95% and its specificity was 92% as compared to the indirect fluorescent antibody test (ifat). the rabbit origin b. piliformis isolate used in this elisa was found to be cross-reactive by ... | 1987 | 3599886 |
| yersinia associated arthritis in shr rats: effect of the microbial status of the host. | following the intravenous injection of live yersinia enterocolitica o:8, 50-69% of shr rats developed arthritis; these rats were antibody free against all rat pathogens tested. in contrast, only 20-25% of shr rats which had serum antibodies against bacillus piliformis, kilham rat virus, and toolan h-1 virus developed arthritis. the results indicate that the microbial load of the host has a profound effect on the susceptibility to experimental arthritis. | 1993 | 8484677 |
| [tyzzer's disease in a pony foal from schleswig-holstein]. | it is reported on a case of tyzzer's disease (infection with bacillus piliformis) in a pony foal in schleswig-holstein. the clinical and pathologic-anatomical findings are described and discussed. | 1995 | 8593776 |
| lesions of experimentally induced tyzzer's disease in syrian hamsters, guineapigs, mice and rats. | the relative susceptibilities of c57bl/6ncr and balb/canncr mice, f344/ncr rats, 2/ncr guineapigs and cr:rgh syrian hamsters to bacillus piliformis infection were determined by orally inoculating 20 weanling females from each species with suspensions of b. piliformis spores. animals from each group were sequentially necropsied over 2 week periods to document the lesions produced. no significant gross or microscopic lesions were observed in the balb mice or the fischer rats. gross and microscopic ... | 1987 | 3599878 |
| diagnosis of subclinical bacillus piliformis infection in a barrier-maintained mouse production colony. | | 1987 | 3437756 |
| clinical and clinicopathologic findings in two foals infected with bacillus piliformis. | bacillus piliformis infection (tyzzer's disease) in foals is rarely observed clinically because of the peracute course of the disease. clinical and clinicopathologic findings as well as information on therapeutic attempts in two foals are described. clinicopathologic abnormalities common to both cases included leukopenia, hyperfibrinogenemia, metabolic acidosis, and hypoglycemia. treatment was unsuccessful in both cases. | 1988 | 3209457 |
| diagnosis of murine infections in relation to test methods employed. | comparative investigations of sendai virus, pneumonia virus of mice (pvm), mouse encephalomyelitis virus (mouse polio), minute virus of mice (mvm), and reovirus type 3 (reo 3) infected murine colonies revealed a 30% higher incidence of positive sera when enzyme-linked immunosorbent assay (elisa) was employed instead of hemagglutination inhibition (hi) tests. equivalent sensitivity as in the elisa was obtained when the same sera were investigated by indirect immunofluorescence (if) tests. the vir ... | 1986 | 3014210 |
| production of tyzzer's disease in rats by ingestion of bacterial spores. | tyzzer's disease was produced in rats by peroral inoculation with spores of bacillus piliformis (tyzzer's organism) of rat origin. after ingestion of 10(6) spores necrotized lesions with intracellular bacterial propagation were seen in the intestines, liver and heart on days 2 to 14 postinoculation (p.i.). a number of b. piliformis were present within enterocytes of the cecum and colon. infected cells were also seen in the liver, myocardium and intestinal muscle layers on days 3 to 7 p.i. infect ... | 1989 | 2739096 |
| a serological indication of the existence of a guineapig poliovirus. | attempts were made to clarify whether laboratory guineapigs may harbour a poliovirus which, in 1911, was described as the cause of a disease called guineapig lameness. by the use of elisa for antibodies against the poliovirus, theiler's murine encephalomyelitis virus (tmev), it was shown that two pet shop guineapigs suffering from lameness had extremely high titres against poliovirus, while healthy guineapigs from the same pet shop were negative. clearly positive results were also found in 35 ou ... | 1997 | 9230501 |
| differences in susceptibility to peroral inoculation with bacillus piliformis spores in rats and mice. | the median liver lesion producing doses of peroral inoculation with the spores of tyzzer's organism rj strain were 10(4. 3) in rats and 10(2. 7) in rats receiving prednisolone treatment for the provocation of tyzzer's disease. in contrast to rats, liver lesions were detected in few mice inoculated perorally with 10(7) spores. in mice inoculated perorally with 10(7) spores, excretion of infective spores in the feces was detected only on day 1 postinoculation. on the other hand, no difference in s ... | 1990 | 2401329 |
| naturally occurring tyzzer's disease in a calf. | naturally occurring clostridium piliforme infection (tyzzer's disease) was found in a calf. light microscopic examination revealed multifocal coagulative necrosis in the liver, catarrhal gastroenteritis, tracheitis and pneumonia, and thymic atrophy. warthin-starry staining clearly showed large filamentous bacilli in bundles or criss-cross patterns within the hepatocytes and epithelium and smooth muscle cells of the ileum and cecum. immunohistochemistry using anti-c. piliforme rt and msk strain a ... | 1999 | 10332834 |
| naturally-occurring tyzzer's disease (bacillus piliformis infection) in commercial rabbits: a clinical and pathological study. | tyzzer's disease has been detected in nine unrelated, commercial rabbitries. during the acute stage of the disease, recently weaned rabbits showed profuse watery diarrhoea. mortality was between 14.2 and 41.2% during the first three weeks of the outbreaks. in surviving animals, there was a chronic evolution with depression, anorexia, loss of weight and sometimes extreme cachexia. reproduction animals were less badly affected. multifocal hepatic necrosis, focal myocardial necrosis, patches of muc ... | 1985 | 4014990 |
| enterotoxaemia involving clostridium perfringens iota toxin in a hysterectomy-derived rabbit colony. | during an explosive outbreak of fatal enteropathic disease involving clostridium perfringens iota (i) toxin, a total of 183 deaths occurred in 18 weeks. the clinical signs and post-mortem findings are reported. examinations for virus, bacillus piliformis and coccidia were negative. clostridium perfringens i toxin was detected in 22 of 27 animals examined (81.5%), but clostridia were not isolated. various treatments were attempted. it is concluded that i toxin and the syndrome described are close ... | 1980 | 6257969 |
| is it possible to cultivate the agent of tyzzer's disease (bacillus piliformis) in cellfree media? | | 1984 | 6485596 |
| studies on the diagnosis of tyzzer's disease in laboratory rat colonies with antibodies against bacillus piliformis (clostridium piliforme). | to develop a system to be used for confirming diagnosis of tyzzer's disease in seropositive rat colonies, 38 mol:sprd, bb/wor/mol-bb, and stroke-prone rats suffering from megaloileitis were examined. all affected rats had been found by a systematic examination of 5-week-old male rats from barrier-protected colonies, sero-positive to bacillus piliformis, the agent of tyzzer's disease. the rats were evaluated by serologic testing, histologic examination of the ileum, liver, and heart (hematoxylin ... | 1994 | 7531255 |
| antigenic diversity in flagellar epitopes among bacillus piliformis isolates. | monoclonal antibodies (mabs) were developed to bacillus piliformis isolate-specific flagellar epitopes and used to group b. piliformis isolates on the basis of epitope expression. balb/c mice immunised with flagella purified from various b. piliformis isolates served as the source of immune spleen cells for fusion with sp2/0ag14 myeloma cells. evaluation of hybridoma culture medium by elisa against various bacterial species and b. piliformis isolates indicated that 482 of 2127 hybridomas secrete ... | 1993 | 7681111 |
| infection of horses by tyzzer's bacillus. | | 1995 | 7774540 |
| seroanalysis of tyzzer's disease in horses: implications that multiple strains can infect equidae. | a monoclonal antibody based competitive inhibition assay was used to detect antibodies in horse sera to purified flagellar antigens from distinct clostridium piliforme isolates. sequential absorption of hyperimmune rat serum to c. piliforme isolate e (horse-origin isolate), a positive c. piliforme-immune horse serum, and other suspected immune horse sera with unrelated bacteria or c. piliforme isolates e or isolate r1 (rat-origin isolate) alone demonstrated the specificity of this assay for c. p ... | 1995 | 7774554 |
| tyzzer's disease in free-living cottontail rabbits (sylvilagus floridanus) in maryland. | complement-fixing (cf) antibody to bacillus piliformis antigen was found in 9 of 14 (64%) serum samples obtained from cottontail rabbits (sylvilagus floridanus) killed in the wild. cf antibody was not present in the serum of 8 cottontail rabbits trapped as juveniles in the same geographic areas and held in captivity for 4 years. sero-negative cottontail rabbits died acutely with lesions typical of tyzzer's disease following the intragastric administration of 10(3.8) eld50 of b. piliformis spores ... | 1976 | 16502694 |
| detection of bacillus piliformis by specific amplification of ribosomal sequences. | in an effort to explore a sensitive species-specific detection system using the polymerase chain reaction (pcr) for b. piliformis, we sequenced 16s ribosomal dna (rdna) of the organism (msk strain) isolated from the mouse and compared it with known rdna sequences of the rj strain isolated from the rat. sequence homology between the msk strain and the rj strain was over 97%, but homology between the msk strain and other bacterial species was less (70-83%). the results indicated that the sequences ... | 1994 | 7925627 |
| variability in the induction of experimental arthritis: yersinia associated arthritis in lewis rats. | lewis rats of different microbiological status were studied for susceptibility to experimentally-induced yersinia associated arthritis. status a rats were serologically positive for bacillus piliformis, kilham rat virus and toolan h-1 virus, whereas status b rats were serologically negative for these same microorganisms. when status a and status b rats were kept in the same room, incidence of arthritis was low (0-13% for status a rats and 27-33% for status b rats). when status b rats only were k ... | 1994 | 8016582 |
| in vitro model of adhesion and invasion by bacillus piliformis. | an in vitro model of bacillus piliformis infection was developed to investigate the mechanisms of adhesion and internalization of this obligate intracellular bacterium. adhesion and internalization events were examined by electron microscopic evaluation of infected caco-2 cell monolayers. a few bacteria were identified in apical surface invaginations and in vacuoles subjacent to the apical surface, whereas the majority of bacteria were observed free within the cytoplasm, suggesting that b. pilif ... | 1993 | 8432608 |
| [infection with bacillus piliformis (tyzzer's disease) in foals]. | tyzzer's disease, caused by bacillus piliformis and characterized by a multifocal, necrotizing hepatitis was diagnosed in a 25 days old, male, german warmblood foal. the animal was submitted to the clinic of horses because of colic symptoms and a severe depression. upon arrival it was comatose, recumbent and showed opisthotonos. due to a severe, metabolic acidosis and a rapid progression of the disease, the foal was euthanatized. in immediately formalin-fixed tissue samples bacillus piliformis w ... | 1993 | 8465178 |
| bacillus piliformis infection (tyzzer's disease) in a patient infected with hiv-1: confirmation with 16s ribosomal rna sequence analysis. | bacillus piliformis is a long, rod-shaped bacterium that has never been grown in cell-free medium and whose taxonomic classification is uncertain. b. piliformis is the causative agent of tyzzer's disease, which is frequently reported in laboratory, wild, and domesticated animals. the spectrum and severity of this disease is wide in animals. although many infections are rapidly fatal, subclinical infections are also common. to date, there have been no reports of b. piliformis infection in human b ... | 1996 | 8655723 |
| a novel presentation of clostridium piliforme infection (tyzzer's disease) in nude mice. | clostridium piliforme infection (tyzzer's disease) was diagnosed in a colony of nude mice. because spontaneous tyzzer's disease had not been reported in nude mice, a study was undertaken to better define the clinicopathologic features of this disease outbreak. sixty homozygous nude (nu/nu) females, 10 nu/nu males, and 10 heterozygous nude (nu/+) females were observed for signs of disease. over a 3-month period, 43% of the nu/nu mice died or manifested clinical signs of disease and were euthanize ... | 1996 | 8699814 |
| naturally occurring tyzzer's disease in cotton-top tamarins (saguinus oedipus). | we noted naturally occurring infection with clostridium piliforme (tyzzer's disease) in 2 captive-reared cotton-top tamarins (saguinus oedipus). spontaneous tyzzer's disease has been reported in multiple species of laboratory, domestic, and wild animals but is extremely rare in humans and nonhuman primates. distinct from idiopathic colitis, which is common in cotton-top tamarins, these 2 tamarins had severe, transmural, necrotizing typhlocolitis accompanied by myocarditis and hepatitis. abundant ... | 2007 | 17348301 |
| the enteritis complex in domestic rabbits: a field study. | a study of the causative agents of enteritis in domestic rabbits from 44 different accessions is described. in descending order of frequency, the organisms most commonly demonstrated were intestinal and hepatic coccidia (eimeria species), escherichia coli, clostridium spp., salmonella, bacillus piliformis, and rotavirus. the species of eimeria identified included those moderately pathogenic and coccidia of low pathogenicity. using seven antisera against known enterpathogenic strains of e. coli, ... | 1993 | 17424177 |
| mutual viral and bacterial infections after housing rats of various breeders within an experimental unit. | fifteen athymic rat strains from 11 breeding colonies were housed within an experimental facility for an immunological study. health status records supplied with 14 of the strains listed infections by kilham's rat virus (krv), clostridium piliforme (bacillus piliformis) and pasteurella pneumotropica for 2, 2 and 1 colonies respectively. in sera taken previous to the study from euthymic rats of 10 strains, antibodies to krv were detected in 3 strains, to pneumonia virus of mice (pvm), rat corona ... | 1996 | 8709572 |
| tyzzer's disease in a eurasian otter (lutra lutra) in scotland. | a eurasian otter (lutra lutra) cub found in weak condition on the isle of harris, scotland, developed bilateral corneal oedema 16 days after being admitted to a rehabilitation centre. it died unexpectedly on day 26. on postmortem examination, there was excess clear fluid in the body cavities and the liver was swollen with numerous pale focal lesions and petechial haemorrhages throughout. histopathological examination revealed bundles of bacilli morphologically typical of clostridium piliforme wi ... | 2008 | 18978367 |
| detection of clostridium piliforme by enzymatic assay of amplified cdna segment in microtitration plates. | we developed a simple enzymatic assay system using reverse transcription-polymerase chain reaction products in a microtitration plate to detect clostridium piliforme in tissue specimens. the reverse transcription-polymerase chain reaction was performed with a biotin-labeled primer directed in a highly conserved area of the c. piliforme 16s rrna gene. the amplified cdna was hybridized to an alkaline phosphatase-labeled dna probe in a microtube, and the mixture was applied to a microtitration well ... | 1996 | 8905580 |
| tyzzer's disease (clostridium piliforme infection) and possible copper toxicity in a lamb. | | 2009 | 19363230 |
| experimentally induced tyzzer's disease in mongolian gerbils (meriones unguiculatus). | tyzzer's disease was induced in outbred, 3- to 4-week-old female mongolian gerbils (meriones unguiculatus) by oral inoculation of either a yolk sac preparation containing spores or a suspension of infected mouse liver. clinical signs and lesions were similar regardless of the inoculum. nests of bacillus piliformis were seen in follicle associated epithelium of ileal peyer's patches by 24 hours post-inoculation. disease severity peaked at the fifty through seventh days. many animals died during t ... | 1984 | 6716957 |
| vertical transmission of bacillus piliformis infection (tyzzer's disease) in a guinea pig: case report. | tyzzer's disease (bacillus piliformis infection) was diagnosed at necropsy in a hysterectomy-derived, gnotobiotically reared guinea pig. this is the first description of spontaneous vertical, presumably transplacental, transmission of b. piliformis infection. subsequent horizontal transmission of the infection was concluded from the presence of specific anti-b. piliformis antibodies in the sera from guinea pigs originating from different litters but reared in the same isolator. | 1984 | 6748596 |
| a study of mouse strains susceptibility to bacillus piliformis (tyzzer's disease): the association of b-cell function and resistance. | tests were conducted on 11 inbred strains of mice and an nih outbred stock. it was found that only the cba/n and c3. cba/n mice (strains deficient in igm production) were highly susceptible to bacillus piliformis infection. susceptibility to infection was determined by induction of typical surface liver lesions and the ability to maintain serial passage without concurrent administration of cortisone. mice deficient in t-cell function (nu/nu/++) were as resistant to bacillus piliformis infection ... | 1981 | 6972467 |
| tularemia, plague, yersiniosis, and tyzzer's disease in wild rodents and lagomorphs in canada: a review. | information related to infection of wild rodents or lagomorphs in canada by francisella tularensis, yersinia pestis, other yersinia spp., and clostridium piliforme was searched for this study. reports on tularemia in humans linked to these species came from diagnostic databases, literature, wildlife health specialists, and public health agencies. tularemia has been diagnosed in 8 species of wild rodent and 2 species in the genus lepus in canada. tularemia occurred in wild animals, or in humans a ... | 2009 | 20190973 |
| studies on tyzzer's disease: comparison between bacillus piliformis strains from mouse, rat and rabbit. | the pathogenicity of bacillus piliformis strains isolated from mouse, rat and rabbit, compared by inoculation into mice, appeared to be identical. the antibody titre obtained for the individual sera from spontaneously infected mice, rats, rabbits, dogs and humans was the same whether the antigen employed was from organisms isolated from mouse, rat or rabbit. | 1980 | 6987456 |
| bacillus piliformis infection (tyzzer's disease) in foals in northwestern united states: a retrospective study of 21 cases. | from jan 1, 1967 through june 1980, 21 cases of bacillus piliformis infection (tyzzer's disease) in foals were diagnosed at the washington animal disease diagnostic laboratory. affected foals died between 7 and 35 days of age. of 4 arabian foals with the disease, 2 also had combined immunodeficiency. | 1981 | 7228786 |
| investigations into an antigen causing tyzzer's disease in mice (bacillus piliformis). | | 1980 | 7234164 |
| development of a monoclonal antibody-based competitive inhibition enzyme-linked immunosorbent assay for detection of bacillus piliformis isolate-specific antibodies in laboratory animals. | a competitive inhibition enzyme-linked immunosorbent assay (elisa) was developed to detect bacillus piliformis isolate-specific antibodies in serum specimens from rats and gerbils experimentally infected with b. piliformis isolates r1, r2, or m. detection was based on the ability of serum antibodies to block binding of b. piliformis isolate-specific monoclonal antibodies to purified b. piliformis flagella. application of this assay to serum specimens collected from sham-infected or experimentall ... | 1994 | 7518016 |
| tyzzer's disease in a red panda (ailurus fulgens fulgens). | a debilitated 9-yr-old female red panda (ailurus fulgens fulgens) with a recent history of corticosteroid administration displayed anorexia, depression, and diarrhea for 2 days. blood work revealed a moderate nonregenerative anemia, leukocytosis, hypokalemia, hyperbilirubinemia, and mildly elevated alanine aminotransferase and aspartate aminotransferase. serology was negative for occult heartworm, toxoplasma gondii, feline leukemia virus, feline infectious peritonitis, feline immunodeficiency vi ... | 2000 | 11428405 |
| risk factors for clostridium piliforme infection in foals. | to determine risk factors for clostridium piliforme infection in neonatal foals on a thoroughbred breeding farm in california. | 2002 | 11918272 |
| detection by pcr of the tyzzer's disease organism (clostridium piliforme) in feces. | we examined whether the tyzzer's disease organism, clostridium piliforme, could be detected in feces by pcr. if the organism could be detected in feces, a diagnosis could be made without sacrifice of the animal. using the rt strain of c. piliforme, we found that a c. piliforme band could be detected when there were > or = 1 x 10(0) bacteria present in the pcr solution, but the presence of fecal extract in the solution depressed the sensitivity 10 fold. nevertheless, we could detect the c. pilifo ... | 2002 | 12451714 |
| tyzzer's disease of rabbits: isolation and propagation of bacillus piliformis (tyzzer) in embryonated eggs. | bacillus piliformis (tyzzer) was isolated from the liver of rabbits with tyzzer's disease and serially passaged in embryonated hens' eggs. weanling rabbits given the 32nd egg passage developed lesions typical of tyzzer's disease and died. b. piliformis was reisolated from the liver of these rabbits in embryonated eggs. outside the host cell, the motile vegetative phase appeared to be unstable, and no means was found to preserve its viability; the results of titrations were believed to be depende ... | 1971 | 16557992 |
| prevalence of naturally occurring viral infections, mycoplasma pulmonis and clostridium piliforme in laboratory rodents in western europe screened from 2000 to 2003. | in this report prevalence rates of rodent viruses in laboratory animals are presented based on routine serological screening of mouse and rat colonies from european institutes. the prevalences found during the period 2000-2003 are compared with those reported for 1981-1984 and 1990-1993. it is shown that some infections were eliminated from laboratory animal colonies (e.g. k-virus and polyomavirus) by taking preventative measures whereas other infections such as mouse hepatitis virus and parvovi ... | 2006 | 16600073 |
| clostridium piliforme infection in two farm-raised white-tailed deer fawns (odocoileus virginianus) and association with copper toxicosis. | necropsy of 2 white-tailed deer fawns who died acutely revealed diarrhea and melena in case no. 1 and no gross changes in case no. 2. histologically, the livers of both deer displayed multifocal coagulative necrosis, with infiltrations of neutrophils, macrophages, and lymphocytes. by warthin-starry staining, bundles of filamentous bacteria were identified within hepatocytes at the periphery of the necrotic foci in case no. 1. there was multifocal myocardiocyte necrosis in case no. 1 and multifoc ... | 2006 | 16966457 |