| metabolism of h2-co2, methanol, and glucose by butyribacterium methylotrophicum. | the fermentative metabolism of butyribacterium methylotrophicum grown on either h2-co2, methanol, glucose, or co is described. the following reaction stoichiometries were obtained: 1.00 h2 + 0.52 co2 leads to 0.22 acetate + 0.06 cell c; 1 methanol + 0.18 co2 + 0.01 acetate leads to 0.24 butyrate + 0.29 cell c; and 1.00 glucose leads to 0.31 co2 + 1.59 acetate + 0.21 butyrate + 0.13 h2 + 1.58 cell c. cell yields of 1.7 g (dry weight) per mol of h2, 8.2 g (dry weight) per mol of methanol, 42.7 g ( ... | 1983 | 6402496 |
| carbon monoxide metabolism of the methylotrophic acidogen butyribacterium methylotrophicum. | the marburg strain of butyribacterium methylotrophicum did not grow on co alone but did consume co during growth on a variety of substrates in the presence of a 100% co gas phase. we selected a strain (the co strain) that grew vigorously on co alone. the ability of the co strain to grow on co was stable through multiple transfers in the absence of co. co dehydrogenase activity was lower in the co strain grown on co (13.3 micromol/min per mg of protein) than in the marburg strain grown on methano ... | 1982 | 7033210 |
| catabolic enzymes of the acetogen butyribacterium methylotrophicum grown on single-carbon substrates. | when grown on formate, formate-co, and methanol-co, butyribacterium methylotrophicum contained high levels of tetrahydrofolate (h4folate) and required enzymes, carbon monoxide dehydrogenase, formate dehydrogenase, and hydrogenase. the activities of methylene-h4folate reductase were comparable to other h4 folate activities (which ranged from 0.55 to 9.28 mumol/min per mg of protein) when measured by an improved procedure. the h4folate activities in formate-grown cells were twice those found in fo ... | 1987 | 3316188 |
| ferredoxin and rubredoxin from butyribacterium methylotrophicum: complete primary structures and construction of phylogenetic trees. | complete amino acid sequences of ferredoxin and rubredoxin from butyribacterium methylotrophicum, a methylotrophic hetero-acetogen, were determined by combination of protease digestion, edman degradation, carboxypeptidase digestion, and/or partial acid hydrolysis. the ferredoxin was composed of 55 amino acids with a molecular weight of 5,732 excluding iron and sulfur atoms and showed a typical 2[4fe-4s]-type ferredoxin sequence with an internal repeat at the 14-23 and 42-51 positions. the rubred ... | 1989 | 2606914 |
| enrichment of microbial electrolysis cell biocathodes from sediment microbial fuel cell bioanodes. | electron-accepting (electrotrophic) biocathodes were produced by first enriching graphite fiber brush electrodes as the anodes in sediment-type microbial fuel cells (smfcs) using two different marine sediments and then electrically inverting the anodes to function as cathodes in two-chamber bioelectrochemical systems (bess). electron consumption occurred at set potentials of -439 mv and -539 mv (versus the potential of a standard hydrogen electrode) but not at -339 mv in minimal media lacking or ... | 2012 | 22610438 |
| phylogeny of human intestinal bacteria that activate the dietary lignan secoisolariciresinol diglucoside. | the human intestinal microbiota is essential for the conversion of the dietary lignan secoisolariciresinol diglucoside (sdg) via secoisolariciresinol (seco) to the enterolignans enterodiol (ed) and enterolactone (el). however, knowledge of the species that catalyse the underlying reactions is scant. therefore, we focused our attention on the identification of intestinal bacteria involved in the conversion of sdg. strains of bacteroides distasonis, bacteroides fragilis, bacteroides ovatus and clo ... | 2006 | 16466386 |
| single-carbon catabolism in acetogens: analysis of carbon flow in acetobacterium woodii and butyribacterium methylotrophicum by fermentation and 13c nuclear magnetic resonance measurement. | the catabolism of methanol, formate, or carbon monoxide to acetate or butyrate or both was examined in two acetogenic bacteria. butyribacterium methylotrophicum simultaneously transformed methanol and formate mainly to butyrate with concomitant h2 and co2 production and consumption. in contrast, methanol plus co was primarily converted to acetate, and only slight amounts of co2 were produced. in vivo 13c nuclear magnetic resonance analysis of [13c]methanol transformation by b. methylotrophicum i ... | 1983 | 6411684 |
| purification and properties of ferredoxin and rubredoxin from butyribacterium methylotrophicum. | a ferredoxin and a rubredoxin from butyribacterium methylotrophicum, which displays a carbonyl-dependent acetyl-coenzyme a synthesis, were purified to electrophoretic homogeneity. the two electron carriers showed absorption spectra similar to those in clostridium species. the ferredoxin displayed absorption peaks at 280 and 391 nm, while rubredoxin displayed absorption peaks at 279, 382, and 482 nm. minimum molecular weights calculated from the respective amino acid compositions were 5,727 for f ... | 1989 | 2548997 |
| the role of tungstate and/or molybdate in the formation of aldehyde oxidoreductase in clostridium thermoaceticum and other acetogens; immunological distances of such enzymes. | besides clostridium thermoaceticum and c. formicoaceticum other resting acetogenic clostridia such as c. aceticum and c. thermoautotrophicum and to a lesser extent non-clostridial acetogens such as butyribacterium methylotrophicum and eubacterium limosum were able to reduce propionate to propanol at the expense of carbon monoxide or formate. methylviologen usually increased the reduction rate. ten microm molybdate in the growth medium decreased this capability for c. thermoaceticum but increased ... | 1992 | 1417415 |
| anaerobic catabolism of formate to acetate and co2 by butyribacterium methylotrophicum. | the catabolism of sodium formate to acetate and carbon dioxide by the anaerobic acetogen butyribacterium methylotrophicum was analyzed by fermentation time course and 13c nuclear magnetic resonance studies. significant hydrogen production and consumption fluxes were observed during formate catabolism but not during the catabolism of formate plus co. in the latter case, formate and co were simultaneously consumed and label distribution studies with mixtures of 13c-labeled co and formate demonstra ... | 1987 | 3106329 |
| methanol bioconversion by butyribacterium methylotrophicum--batch fermentation yield and kinetics. | butyribacterium methylotrophicum is an anaerobic bacterium that can convert methanol to butyrate. this ability to produce longer-chain carbon compounds from c(1) substrates could be of commercial significance. the fermentation rates and product formation depend on the methanol/bicarbonate ratios during fermentation. the kinetics of batch fermentation fit the luedeking-piret model with growth and maintenance associated product formation. butyrate yield of 0.256 mol/mol methanol (ca. 85% of theore ... | 1983 | 18548714 |
| effect of surfactants on carbon monoxide fermentations by butyribacterium methylotrophicum. | butyribacterium methylotrophicum has been grown on carbon monoxide as its carbon and energy source in the presence of various surfactants that are capable of forming microbubble dispersions for the screening of surfactants for use in microbubble-sparged synthesis gas fermentations. in the range of 0-3 times the critical micelle concentration, the presence of tween surfactants was not significantly inhibitory to growth, final cell density, and fermentation stoichiometry, although some of the brij ... | 1997 | 18576119 |
| mass-transfer properties of microbubbles. 1. experimental studies. | synthesis-gas fermentations have typically been gas-to-liquid mass-transfer-limited due to low solubilities of the gaseous substrates. a potential method to enhance mass-transfer rates is to sparge with microbubble dispersions. mass-transfer coefficients for microbubble dispersions were measured in a bubble column. oxygen microbubbles were formed in a dilute tween 20 solution using a spinning disk apparatus. axial dispersion coefficients measured for the bubble column ranged from 1.5 to 7.2 cm2/ ... | 2013 | 9496668 |
| isolation and screening of carboxydotrophs isolated from composts and their potential for butanol synthesis. | carboxydotrophs are known for their ability to convert carbon monoxide (co) to butanol through fermentation. such a platform offers a promising alternative approach to biofuel production from synthesis gas feedstocks. in this study, carboxydotrophs were isolated from various manure compost. out of 500 isolates, only 11 carboxydotrophs (7 mesophiles and 4 thermophiles) were found to utilize co as the sole source of carbon and energy. to assess the biochemical basis for their ability to produce bi ... | 2017 | 24350453 |
| genome sequence of the acetogenic bacterium butyribacterium methylotrophicum dsm 3468t. | butyribacterium methylotrophicum dsm 3468t is an acetogenic methylotrophic, anaerobic, carbon monoxide-oxidizing bacterium that produces acetate, butyrate, and butanol. the genome consists of a single chromosome (4.3 mb) and harbors 3,989 predicted protein-encoding genes. | 2016 | 27908997 |