| cloning and sequencing of a cyclodextrin glucanotransferase gene from bacillus ohbensis and its expression in escherichia coli. | a cyclodextrin glucanotransferase (cgtase) gene of bacillus ohbensis was cloned in escherichia coli and the nucleotide sequence was determined. a single open reading frame (2112 bp) with a ttg codon as an initiator was identified that encodes a typical signal peptide of 29 amino acids followed by the mature enzyme (675 amino acids), of which the partial amino acid sequences of the n-terminal region and some lysyl-endopeptidase fragments were determined by edman degradation. the cgtase gene was e ... | 1991 | 1368710 |
| characterization of five isomers of branched cyclomaltoheptaose (beta cd) having degree of polymerization (d.p.) = 9: reinvestigation of three positional isomers of diglucosyl-beta cd. | it has been confirmed by methylation analyses and chemical syntheses that three isomers of branched cyclomaltoheptaose (beta cd) isolated from the mother liquors of a large-scale preparation of beta cd with bacillus ohbensis cyclomaltodextrin glucanotransferase are 6(1),6(4)-di-o-(alpha-d-glucopyranosyl)-cyclomaltoheptaose (1), 6(1),6(3)-di-o-(alpha-d-glucopyranosyl)-cyclomaltoheptaose (2), and 6-o-(alpha-isomaltosyl)-cyclomaltoheptaose (4) instead of 6(1),6(2)-di-o-(alpha-d-glucopyranosyl)-cycl ... | 1991 | 1786577 |
| isolation and characterization of three positional isomers of diglucosylcyclomaltoheptaose. | three positional isomers of diglucosylcyclomaltoheptaose [(g)2-beta-cyclodextrin], 6(1),6(4)-di-o-(a-d-glucopyranosyl)-cyclomaltoheptaose (1), 6(1),6(3)-di-o-(a-d-glucopyranosyl)-cyclomaltoheptaose (2), and 6(1),6(2)-di-o-(a-d-glucopyranosyl)-cyclomaltoheptaose (3) were isolated by h.p.l.c. on a reversed-phase column from the mother liquors of a large-scale preparation of beta cd with bacillus ohbensis cyclomaltodextrin glucanotransferase (ec 2.4.1.19) and were characterized by h.p.l.c. analysis ... | 1990 | 2208187 |
| replacement of an amino acid residue of cyclodextrin glucanotransferase of bacillus ohbensis doubles the production of gamma-cyclodextrin. | cyclodextrin glucanotransferase (cgtase; ec 2.4.1.19) produces cyclodextrin (cd) from starch through an intramolecular transglucosylation reaction. to obtain a better understanding of the amylolytic and cyclization mechanisms of cgtase, and furthermore to improve the production of gamma-cd, mutant cgtases were constructed by site-directed mutagenesis of the cgtase gene of bacillus ohbensis replacing tyr at position 188 by 19 other amino acids. all mutant enzymes retained both starch-degrading an ... | 1994 | 7764720 |
| regulation of cyclodextrin glucanotransferase synthesis in bacillus ohbensis. | the production of cyclodextrin glucanotransferase (cgtase) by bacillus ohbensis is dependent on the presence of starch and inhibited by glucose in the medium. northern blot analysis revealed that the cgtase gene (cgt) was transcribed to almost the same level irrespective of the presence or absence of starch, but glucose completely repressed the transcription. furthermore, a relatively high amount of cgtase protein was detected on western blotting only in the medium with starch, showing the lack ... | 1997 | 9141663 |
| transcriptional regulation of the bacillus ohbensis cyclodextrin glucanotransferase gene in b. subtilis. | the expression of the cyclodextrin glucanotransferase (cgtase) gene (cgt) of bacillus ohbensis, when introduced into an alpha-amylase-defective strain of b. subtilis on a multicopy plasmid, phy300plk, was induced in the presence of starch and was subject to catabolite repression by glucose as well as in the original strain, b. ohbensis. we constructed a cgt'::'lacz translational fusion to study the expression in b. subtilis, and this construct was confirmed to be subject to both starch induction ... | 1999 | 10635555 |
| extracellular production of bacillus ohbensis cyclodextrin glucanotransferase by b. subtilis. | | 1993 | 27314803 |