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a special report: four-year study of a boy with combined immune deficiency maintained in strict reverse isolation from birth.a 4-year study of a boy with combined immune deficiency is presented, and the impact of this disease on various aspects of his growth and development is examined. there is no evidence of immune deficiency in either parent or in the genetic background on the maternal side. three children of a brother of the mother's father may have had immune deficiencies but two have grown to be teenagers with no problems. another died. at autopsy, however, lymph nodes appeared normal. the deceased older brother ...1977401538
isolation of galantins i and ii, water-soluble basic peptides. studies on antibiotics from the genus bacillus. iii.two water-soluble basic antibiotics named galantins i and ii were isolated from a strain resembling bacillus pulvifaciens. both antibiotics are peptides containing glycine, alanine, ornithine, lysine and some unknown ninhydrin-positive components. an approximate empirical formula c50 plus and minus h98plus and minus 2o17n16 is indicated for galantin i. these are active against some gram-positive, acid-fast and gram-negative bacteria.19751112763
aerobic mesophilic and psychrotrophic sporeforming bacteria in buffalo milk.seasonal variation of the population of aerobic sporeformers in raw milk was higher in summer than in other seasons. least variation was in fall, but variation in winter and spring was similar. aerobic mesophilic sporeformers in raw milk consisted mainly of bacillus subtilis (42.5%) and bacillus megaterium (34.8%), followed by bacillus circulans (4.9%), bacillus cereus (4.6%), bacillus pumilus (2.9%), bacillus polymyxa (2.8%), bacillus licheniformis (1.9%), bacillus badius (1.5%), bacillus brevi ...19836886164
reclassification of paenibacillus (formerly bacillus) pulvifaciens (nakamura 1984) ash et al. 1994, a later subjective synonym of paenibacillus (formerly bacillus) larvae (white 1906) ash et al. 1994, as a subspecies of p. larvae, with emended descriptions of p. larvae as p. larvae subsp. larvae and p. larvae subsp. pulvifaciens.a polyphasic taxonomic study of four strains of paenibacillus larvae and four strains of paenibacillus pulvifaciens (including duplicates of both type strains) supported the reclassification of both former bacillus species into one species, p. larvae. our conclusions were based on morphological and analytab products (api) tests, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) of whole-cell proteins, gas chromatography of methylated fatty acids, pyrolysis mass spectrometry, d ...19968573507
paenibacillus apiarius sp. nov.the name "bacillus apiarius" katznelson 1955 was not included on the approved lists of bacterial names and thus lost standing in bacterial nomenclature. the genetic homogeneity of "b. apiarius" strains was assessed by determining their g+c contents by the buoyant density method and by measuring the levels of dna relatedness by spectrophotometric reassociation procedures. the g+c contents of the 15 strains examined, ranged from 52 to 54 mol%. dna reassociation revealed the presence of two cluster ...19968782677
a polyphasic reassessment of the genus paenibacillus, reclassification of bacillus lautus (nakamura 1984) as paenibacillus lautus comb. nov. and of bacillus peoriae (montefusco et al. 1993) as paenibacillus peoriae comb. nov., and emended descriptions of p. lautus and of p. peoriae.seventy-seven strains representing 10 species in the paenibacillus polymyxa 16s rrna group and 3 other species that exhibit phenetic relatedness to members of this group, bacillus lautus, "bacillus longisporus," and bacillus peoriae, were characterized genotypically and phenotypically by performing an amplified ribosomal dna restriction analysis, a randomly amplified polymorphic dna analysis, a fatty acid methyl ester analysis, sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-c ...19968863428
reclassification of "bacillus pulvifaciens" group ii as brevibacillus agri.organisms formerly identified as strains of paenibacillus pulvifaciens group ii were reclassified as members of the species brevibacillus agri. the reclassification was based on phylogenetic analysis of 16s rrna gene sequences from selected bacillaceae. the analysis strongly supported placement of group ii strains in the genus brevibacillus. high dna relatedness of 87-100% and close phenotypic similarity demonstrated that group ii strains were members of the species brevibacillus agri.19979058544
[tracing the past half century of my microbial transformation studies].in the middle of 1950's, microbial transformation technology was introduced into the field of synthetic chemistry as a new methodology. there was a sudden interest in research on the problems of producing steroid hormones by microbial transformation. at that time, the first project entitled "the study for microbial transformation of steroids", the "tsuda project", was established in the institute of applied microbiology (iam), university of tokyo, in the spring 1956, in which i took part. this p ...200011082697
differentiation of paenibacillus larvae subsp. larvae, the cause of american foulbrood of honeybees, by using pcr and restriction fragment analysis of genes encoding 16s rrna.a rapid procedure for the identification of paenibacillus larvae subsp. larvae, the causal agent of american foulbrood (afb) disease of honeybees (apis mellifera l.), based on pcr and restriction fragment analysis of the 16s rrna genes (rdna) is described. eighty-six bacterial strains belonging to 39 species of the genera paenibacillus, bacillus, brevibacillus, and virgibacillus were characterized. amplified rdna was digested with seven restriction endonucleases. the combined data from restricti ...200212089057
a pcr-based method that permits specific detection of paenibacillus larvae subsp. larvae, the cause of american foulbrood of honey bees, at the subspecies level.a reliable procedure for the identification of paenibacillus larvae subsp. larvae, the causal agent of american foulbrood disease of honey bees (apis mellifera l.) based on the polymerase chain reaction (pcr) and subspecies - specific primers is described.200415189284
bacillus pulvifaciens (n. sp.), an organism associated with powdery scale of honeybee larvae. 195015421942
proposal to reclassify paenibacillus larvae subsp. pulvifaciens dsm 3615 (atcc 49843) as paenibacillus larvae subsp. larvae. results of a comparative biochemical and genetic study.the bacterial pathogen paenibacillus larvae subsp. larvae (p. l. larvae), is the etiological agent of american foulbrood, an extremely contagious and disastrous disease of honeybee brood. in case of american foulbrood the destruction of infected colonies is often considered the only workable control measure. therefore, the ability to diagnose this disease properly is important to prevent unnecessary economic loss to beekeepers. the development of suitable methods for the early and reliable detec ...200415530737
reclassification of paenibacillus larvae subsp. pulvifaciens and paenibacillus larvae subsp. larvae as paenibacillus larvae without subspecies differentiation.a polyphasic taxonomic study of the two subspecies of paenibacillus larvae, paenibacillus larvae subsp. larvae and paenibacillus larvae subsp. pulvifaciens, supported the reclassification of the subspecies into one species, paenibacillus larvae, without subspecies separation. our conclusions are based on the analysis of six reference strains of p. larvae subsp. pulvifaciens and three reference strains and 44 field isolates of p. larvae. subsp. larvae. the latter originated from brood or honey of ...200616514018
diagnosis of american foulbrood in honey bees: a synthesis and proposed analytical protocols.worldwide, american foulbrood (afb) is the most devastating bacterial disease of the honey bee (apis mellifera). because the distinction between afb and powdery scale disease is no longer considered valid, the pathogenic agent has recently been reclassified as one species paenibacillus larvae, eliminating the subspecies designations paenibacillus larvae subsp. larvae and paenibacillus larvae subsp. pulvifaciens. the creamy or dark brown, glue-like larval remains of infected larvae continue to pr ...200617083701
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