| how close is close: 16s rrna sequence identity may not be sufficient to guarantee species identity. | 16s rrna (genes coding for rrna) sequence comparisons were conducted with the following three psychrophilic strains: bacillus globisporus w25t (t = type strain) and bacillus psychrophilus w16at, and w5. these strains exhibited more than 99.5% sequence identity and within experimental uncertainty could be regarded as identical. their close taxonomic relationship was further documented by phenotypic similarities. in contrast, previously published dna-dna hybridization results have convincingly est ... | 1992 | 1371061 |
| thermal analysis of bacteria by differential scanning calorimetry: relationship of protein denaturation in situ to maximum growth temperature. | differential scanning calorimetry (dsc) was used to analyze thermal transitions in two strains of the thermophile bacillus stearothermophilus (atcc 12016 and wat), the mesophile bacillus megaterium and the psychrotroph bacillus psychrophilus. the observed transitions, representing lipid melting and dna and protein unfolding, are compared to the maximum growth temperature (tmax) in each species as a means of identifying critical, thermolabile targets responsible for heat-induced inhibition of gro ... | 1990 | 2121283 |
| chemical analysis of cell walls and autolytic digests of bacillus psychrophilus. | the amino acid composition of isolated cell walls of bacillus psychrophilus has been determined before and after extraction of protein with ethylenediamine-tetraacetic acid at 45 c. this revealed that the peptidoglycan consists of ala, lys, and glu in a molar ratio of 3:1:2. by using autolytic digests of log-phase cell walls, it was possible to detect 14 ninhydrin-positive degradation products. chemical analyses of the seven major bands from these digests indicated that the amino acid sequence o ... | 1973 | 4197901 |
| effect of temperature on the cellular integrity of bacillus psychrophilus. | | 1972 | 4563852 |
| effect of temperature on the integrity of bacillus psychrophilus cell walls. | cell walls isolated from bacillus psychrophilus autolyse at temperatures which support growth. at temperatures above the maximum growth temperature (28 c), a nonenzymatic lysis occurs. removal of autolytic enzyme activity with 10 m licl had little effect on the rate or extent of lysis at elevated temperatures (37 and 45 c). nonenzymatic lysis was characterized chemically by a decrease in the liberation of n-terminal groups, and the effects of ph, ca(2+), and ethylenediaminetetraacetic acid sugge ... | 1972 | 4621683 |
| the effect of temperature on lysis of cells and cell walls of bacillus psychrophilus. | | 1971 | 5114551 |
| comparative effect of temperature on the oxidative metabolism of whole and disrupted cells of a psychrophilic and a mesophilic species of bacillus. | we investigated the influence of temperature, in the range of 45 to 5 c, on the rate of oxidation of glucose and citrate by intact cells and cell-free extracts of psychrophilic bacillus psychrophilus and mesophilic b. thuringiensis. both glucose and endogenous oxidation by whole and disrupted cells of the psychrophile decreased more slowly with decrease in temperature than did glucose and endogenous oxidation by whole and disrupted cells of the mesophile. similar results were obtained for the ox ... | 1968 | 5636832 |
| cold-sensitive mutant with defective growth at 5 degrees c from a psychrotrophic bacterium. | a cold-sensitive (cs) mutant of the psychrotroph, bacillus psychrophilus, was obtained by n-methyl-n'-nitro-n-nitrosoguanidine mutagenization and penicillin counterselection. in the presence of citrate, the wild-type grew well at both 5 and 20 degrees c whereas the cs mutant grew well at 20 degrees c (the permissive temperature) but, at 5 degrees c (the restrictive temperature), grew at a reduced rate for two to three generations followed by a complete plateau in growth. upon return of the cs mu ... | 1980 | 6447536 |
| fatty acid composition and primer specificity of de novo fatty acid synthetase in bacillus globispores, bacillus insolitus, and bacillus psychrophilus. | the fatty acid compositions of three psychrophilic species of bacillus were determined by gas--liquid chromatography. the proportions of straight-chain fatty acids, branched-chain fatty acids, and unsaturated fatty acids were found to be 13.3, 86.7, and 26.1% of the total cellular fatty acids for bacillus globispores, 36.6, 63.4, and 25.1% for bacillus insolitus, and 6.9, 93.1, and 18.4% for bacillus psychrophilus, respectively. in all three organisms the de novo fatty acid synthetase specificit ... | 1983 | 6673817 |
| phylogenetic interrelationships of round-spore-forming bacilli containing cell walls based on lysine and the non-spore-forming genera caryophanon, exiguobacterium, kurthia, and planococcus. | the 16s rrna gene sequences of "bacillus aminovorans" and several species considered to be phylogenetically related to the group 2 bacilli of ash et al. (c. ash, j. a. e. farrow, s. wallbanks, and m. d. collins, lett. appl. microbiol. 13:202-206, 1991) were determined. a comparative analysis of the sequence data revealed that the round-spore-forming group 2 bacilli, together with some asporogenous taxa (the genera caryophanon, exiguobacterium, kurthia, planococcus), form a phylogenetically disti ... | 1994 | 8123563 |
| protein-coding genes as molecular markers for ecologically distinct populations: the case of two bacillus species. | bacillus globisporus and bacillus psychrophilus are one among many pairs of ecologically distinct taxa that are distinguished by very few nucleotide differences in 16s rrna gene sequence. this study has investigated whether the lack of divergence in 16s rrna between such species stems from the unusually slow rate of evolution of this molecule, or whether other factors might be preventing neutral sequence divergence at 16s rrna as well as every other gene. b. globisporus and b. psychrophilus were ... | 2000 | 10843041 |
| sporosarcina aquimarina sp. nov., a bacterium isolated from seawater in korea, and transfer of bacillus globisporus (larkin and stokes 1967), bacillus psychrophilus (nakamura 1984) and bacillus pasteurii (chester 1898) to the genus sporosarcina as sporosarcina globispora comb. nov., sporosarcina psychrophila comb. nov. and sporosarcina pasteurii comb. nov., and emended description of th. | a light-orange-coloured, facultatively anaerobic, rod-shaped bacterium (strain sw28t), which was isolated from seawater in korea, was taxonomically studied by a polyphasic approach. this organism formed round terminal endospores in swollen sporangia. the peptidoglycan type is a4alpha, based on l-lys-l-ala-d-asp. the predominant menaquinone is mk-7 and the major fatty acid is ante-c15:0. the g+c content of the dna is 40 mol%. phylogenetic analysis based on 16s rdna sequences showed that strain sw ... | 2001 | 11411676 |
| cytidine deaminase from two extremophilic bacteria: cloning, expression and comparison of their structural stability. | we cloned, purified and characterized two extremophilic cytidine deaminases: cda(bcald) and cda(bpsy), isolated from bacillus caldolyticus (growth at 72 degrees c) and bacillus psychrophilus (growth at 10 degrees c), respectively. we compared their thermostability also with the mesophilic counterpart, cda(bsubt), isolated from bacillus subtilis (growth at 37 degrees c). the dna fragments encoding cda(bcald) and cda(bpsy) were sequenced and the deduced amino acid sequences showed 70% identity. hi ... | 2001 | 11739901 |
| phylogenetic relationships between bacillus species and related genera inferred from comparison of 3' end 16s rdna and 5' end 16s-23s its nucleotide sequences. | the nucleotide sequences of the 3' end of the 16s rdna and the 16s-23s internal transcribed spacer (its) of 40 bacillaceae species were determined. these included 21 bacillus, 9 paenibacillus, 6 brevibacillus, 2 geobacillus, 1 marinibacillus and 1 virgibacillus species. comparative sequence analysis of a 220 bp region covering a highly conserved 150 bp sequence located at the 3' end of the 16s rrna coding region and a conserved 70 bp sequence located at the 5' end of the 16s-23s its of the 40 sp ... | 2003 | 12807189 |
| reversible filamentous growth in the psychrophile bacillus psychrophilus. | | 1976 | 975860 |
| reciprocal influence of protein domains in the cold-adapted acyl aminoacyl peptidase from sporosarcina psychrophila. | acyl aminoacyl peptidases are two-domain proteins composed by a c-terminal catalytic α/β-hydrolase domain and by an n-terminal β-propeller domain connected through a structural element that is at the n-terminus in sequence but participates in the 3d structure of the c-domain. we investigated about the structural and functional interplay between the two domains and the bridge structure (in this case a single helix named α1-helix) in the cold-adapted enzyme from sporosarcina psychrophila (spaap) u ... | 2013 | 23457536 |
| structural investigation of the cold-adapted acylaminoacyl peptidase from sporosarcina psychrophila by atomistic simulations and biophysical methods. | protein structure and dynamics are crucial for protein function. thus, the study of conformational properties can be very informative for characterizing new proteins and to rationalize how residue substitutions at specific protein sites affect its dynamics, activity and thermal stability. here, we investigate the structure and dynamics of the recently isolated cold-adapted acylaminoacyl peptidase from sporosarcina psychrophila (spaap) by the integration of simulations, circular dichroism, mass s ... | 2014 | 25280393 |
| complete genome sequence of the sporosarcina psychrophila dsm 6497, a psychrophilic bacillus strain that mediates the calcium carbonate precipitation. | sporosarcina psychrophila dsm 6497 is a gram positive, spore-formation psychrophilic bacterial strain, widely distributed in terrestrial and aquatic environments. here we report its complete sequence including one circular chromosome of 4674191bp with a gc content of 40.3%. genes encoding urease are predicted in the genome, which provide insight information on the microbiologically mediated urea hydrolysis process. this urea hydrolysis can further lead to an increase of carbonate anion and alkal ... | 2016 | 27015981 |
| a bacterial acyl aminoacyl peptidase couples flexibility and stability as a result of cold adaptation. | life in cold environments requires an overall increase in the flexibility of macromolecular and supramolecular structures to allow biological processes to take place at low temperature. conformational flexibility supports high catalytic rates of enzymes in the cold but in several cases is also a cause of instability. the three-dimensional structure of the psychrophilic acyl aminoacyl peptidase from sporosarcina psychrophila (spaap) reported in this paper highlights adaptive molecular changes res ... | 2016 | 27739253 |
| influence of temperature on the effectiveness of a biogenic carbonate surface treatment for limestone conservation. | so far, most studies on microbiologically induced carbonate precipitation for limestone conservation have been performed at temperatures optimal for the activity of the calcinogenic bacteria (i.e., 20-28 °c). successful application in practice, however, requires adequate performance in a wide range of environmental conditions. therefore, the aim of this study was to select microorganisms that are most suited for biodeposition at temperatures relevant for practice. in a first step, ureolytic micr ... | 2013 | 22426701 |
| cold active hydrolytic enzymes production by psychrotrophic bacilli isolated from three sub-glacial lakes of nw indian himalayas. | the diversity of culturable, cold-active enzymes producing bacilli was investigated from three sub-glacial lakes of north western indian himalayas. amplified ribosomal dna restriction analysis (ardra) using three restriction enzymes alu i, msp i, and hae iii led to the clustering of 136 bacilli into 26, 23, and 22 clusters at 75% similarity index from chandratal lake, dashair lake, and pangong lake, respectively. phylogenetic analysis based on 16s rrna gene sequencing led to the identification o ... | 2016 | 26933936 |
| promiscuity, stability and cold adaptation of a newly isolated acylaminoacyl peptidase. | we report on the characterisation of a member of the acylaminoacyl peptidase family, the first isolated from bacteria. the enzyme was obtained from the psychrophilic bacterium sporosarcina psychrophila and shows the typical features of cold adaptation (low t(m), optimal temperature of 40 °c, poor thermal stability). it was also tested for substrate specificity, effect of metals, temperature dependence and structure stability and revealed promiscuous catalytic activity on at least two chemically ... | 2011 | 21635934 |
| sporosarcina macmurdoensis sp. nov., from a cyanobacterial mat sample from a pond in the mcmurdo dry valleys, antarctica. | strain cms 21w(t) was isolated from a cyanobacterial mat sample taken from a pond located in the mcmurdo dry valleys, antarctica. based on its phenotypic, chemotaxonomic and phylogenetic properties, strain cms 21w(t) was identified as a member of the genus sporosarcina: at the 16s rrna gene level, cms 21w(t) exhibited about 93-96 % similarity to all reported species of sporosarcina and exhibited a maximum similarity of 96 % to both sporosarcina globispora and sporosarcina psychrophila. based on ... | 2003 | 13130019 |
| attached and unattached microbial communities in a simulated basalt aquifer under fracture- and porous-flow conditions. | bench scale column studies were used to examine the partitioning of microorganisms between groundwater and a geologic medium and to examine the effect of hydrogeology (i.e., porous- versus fracture-flow) on organism partitioning. replicated columns were constructed with intact basalt core segments that contained natural fractures and with the same basalt crushed into particles. the columns were perfused with groundwater, and upon reaching a steady state, the columns were sacrificed and the attac ... | 2001 | 11375197 |
| phylogeny of bacillus sphaericus-like organisms. | the mesophilic round-spored bacteria embrace four species, namely bacillus sphaericus, bacillus fusiformis, bacillus silvestris and bacillus pasteurii. although not displayed by all strains, mosquito pathogenicity is a noteworthy characteristic of b. sphaericus sensu lato. phylogenetic analysis based on 16s rdna sequences from 58 strains identified as b. sphaericus was used to examine the genetic heterogeneity of the taxon. results from sequence analysis were compared with whole-cell fatty acid ... | 2000 | 11034479 |
| molecular cloning of the genes for pyruvate kinase of two bacilli, bacillus psychrophilus and bacillus licheniformis, and comparison of the properties of the enzymes produced in escherichia coli. | the genes for the pyruvate kinases of a psychrophile, bacillus psychrophilus, and a mesophile, bacillus licheniformis, have been cloned in escherichia coli, and all their nucleotides were sequenced. the two bacterial enzymes each had an extra c-terminal sequence consisting of about 110 amino acid residues, which has been found in the b. stearothermophilus enzyme. both enzymes were overexpressed in e. coli and the properties of the purified enzymes were compared to those of the b. stearothermophi ... | 1995 | 7549104 |