| chitinolytic properties of bacillus pabuli k1. | the chitinolytic properties of bacillus pabuli k1 isolated from mouldy grain was studied. chitinase activity was measured as the release of p-nitrophenol from p-nitrophenyl-n,n'-diacetylchitobiose. influences of substrate concentration and different environmental variables on growth and chitinase activity were determined. the optimum environmental conditions for chitinase production were: 30 degrees c, initial ph 8, initial oxygen 10% and aw > 0.99. chitinase production was induced when b. pabul ... | 1994 | 8200864 |
| survey of bacterial populations present in us-produced linerboard with high recycle content. | to survey paperboard products from 17 us mills for bacterial populations and for bacteria potentially harmful to human health. | 2010 | 19645765 |
| A Rapid Microbioassay for Discovery of Antagonistic Bacteria for Phytophthora parasitica var. nicotianae. | A simple, rapid, small-scale microbioassay for infection of tobacco seedlings by Phytophthora parasitica var. nicotianae was developed here. This assay uses tobacco seedlings cultivated in Petri dishes for a standardized method for quantitation of initial zoospore inocula and high-throughput screening of antagonistic bacteria. Zoospore inocula between 102 to 105 spores per Petri dish were inoculated on 14-day-old tobacco seedlings for the susceptibility test. One thousand zoospores' treatment wa ... | 2011 | 22046969 |
| secretion of cyclodextrin glucanotransferase in e. coli using bacillus subtilis lipase signal peptide and optimization of culture medium. | the cyclodextrin glycosyltransferase (cgtase) of paenibacillus pabuli us132 was fused to the secretive lipase signal peptide of b. subtilis. this leads to an efficient secretion of the recombinant enzyme into the culture medium of e. coli as an active and soluble form contrasting with the native construction leading to a periplasmic production. in order to enhance the yield of cgtase production, an experimental design methodology was applied for the optimization of the culture composition. hence ... | 2012 | 22279945 |
| paenibacillus taichungensis sp. nov., from soil in taiwan. | among a large collection of taiwanese soil isolates, a novel gram-variable, rod-shaped, motile, endospore-forming bacterial strain, strain v10537(t), was subjected to a polyphasic study including 16s rrna and gyrb gene sequence analysis, dna-dna hybridization experiments, cell wall peptidoglycan type, cellular fatty acid composition analysis and comparative phenotypic characterization. 16s rrna gene sequence analysis indicated that the organism belonged to the genus paenibacillus. strain v10537( ... | 2008 | 18984707 |
| the cyclodextrin glycosyltransferase of paenibacillus pabuli us132 strain: molecular characterization and overproduction of the recombinant enzyme. | the gene encoding the cyclodextrin glycosyltransferase (cgtase) of paenibacillus pabuli us132, previously described as efficient antistaling agent and good candidate for cyclodextrins production, was cloned, sequenced, and expressed in escherichia coli. sequence analysis showed that the mature enzyme (684 amino acids) was preceded by a signal peptide of 34 residues. the enzyme exhibited the highest identity (94%) to the beta-cgtase of bacillus circulans no. 8. the production of the recombinant c ... | 2008 | 18704190 |
| paenibacillus provencensis sp. nov., isolated from human cerebrospinal fluid, and paenibacillus urinalis sp. nov., isolated from human urine. | gram-negative, spore-forming rods were isolated from a human urine sample (strain 5402403(t)) and a human cerebrospinal fluid sample (strain 4401170(t)). based on genotypic characteristics, these strains belonged to the genus paenibacillus and were closely related. phylogenetic analysis based on 16s rrna gene sequence comparison showed that they clustered with paenibacillus massiliensis 2301065(t) (95.9 and 94.3 % 16s rrna gene sequence similarity, respectively, for strains 5402403(t) and 440117 ... | 2008 | 18319478 |
| production of chitinolytic enzymes by a strain (bm17) of paenibacillus pabuli isolated from crab shells samples collected in the east sector of central tyrrhenian sea. | nineteen bacterial isolates were grown in shaken cultures in media containing chitin as carbon source and different additional nitrogen sources such as yeast nitrogen base (ynb), yeast extract (ye), corn steep liquor (csl) and ammonium sulfate. strain bm17 showed the highest activity (200 u/l) in medium containing chitin (1%) and ynb (0.5%). molecular analysis of the 16s rrna gene showed that strain bm17 belongs to the species paenibacillus pabuli (99.72% homology). the enzyme activity started a ... | 2008 | 18076982 |
| characterization and three-dimensional structures of two distinct bacterial xyloglucanases from families gh5 and gh12. | the plant cell wall is a complex material in which the cellulose microfibrils are embedded within a mesh of other polysaccharides, some of which are loosely termed "hemicellulose." one such hemicellulose is xyloglucan, which displays a beta-1,4-linked d-glucose backbone substituted with xylose, galactose, and occasionally fucose moieties. both xyloglucan and the enzymes responsible for its modification and degradation are finding increasing prominence, reflecting both the drive for enzymatic bio ... | 2007 | 17376777 |
| paenibacillus panacisoli sp. nov., a xylanolytic bacterium isolated from soil in a ginseng field in south korea. | a gram-positive, facultatively anaerobic, motile, spore-forming bacterium, designated gsoil 1411t, was isolated from soil of a ginseng field in pocheon province (south korea) and was characterized using a polyphasic approach. comparative analysis of 16s rrna gene sequences revealed that strain gsoil 1411t belongs to the family paenibacillaceae, with closest sequence similarity to the type strains of paenibacillus xylanilyticus (95.7%), paenibacillus illinoisensis (95.2%) and paenibacillus pabuli ... | 2006 | 17082410 |
| paenibacillus barcinonensis sp. nov., a xylanase-producing bacterium isolated from a rice field in the ebro river delta. | a gram-positive, endospore-forming, xylanase-producing bacterium isolated from a rice field was studied taxonomically. the strain grows at 10-40 degrees c and in the presence of lysozyme or 5 % (w/v) nacl. chemotaxonomic analysis revealed that mk-7 was the predominant menaquinone of the isolated strain, while the major fatty acid was anteiso-c(15 : 0). comparison of 16s rrna gene sequences showed that strain bp-23(t) fell within the radiation of the cluster comprising paenibacillus species. the ... | 2005 | 15774688 |
| paenibacillus xylanilyticus sp. nov., an airborne xylanolytic bacterium. | during a search for xylan-degrading micro-organisms, a sporulating bacterium was recovered from xylan-containing agar plates exposed to air in a research laboratory (salamanca university, spain). the airborne isolate (designated strain xil14t) was identified by 16s rrna gene sequencing as representing a paenibacillus species most closely related to paenibacillus illinoisensis jcm 9907t (99.3 % sequence similarity) and paenibacillus pabuli dsm 3036t (98 % sequence similarity). phenotypic, chemota ... | 2005 | 15653909 |
| isolation and 16s dna characterization of soil microorganisms from tropical soils capable of utilizing the herbicides hexazinone and tebuthiuron. | six non-fermentative bacteria were isolated from colombian (south america) and hawaiian (usa) soils after enrichment with minimal medium supplemented with two herbicides, hexazinone (hex) and tebuthiuron (teb). microscopic examination and physiological tests were followed by partial 16s dna sequence analysis, using the first 527 bp of the 16s rrna gene for bacterial identification. the isolated microorganisms (and in brackets, the herbicide that each degraded) were identified as: from colombia. ... | 2003 | 14649709 |
| evaluation of a chromogenic chito-oligosaccharide analogue, p-nitrophenyl-beta-d-n,n'-diacetylchitobiose, for the measurement of the chitinolytic activity of bacteria. | three methods of quantifying chitinase activity were compared. the activities of crude chitinases of 10 bacterial isolates from different environments were estimated in terms of (1) the release of p-nitrophenol from the chromogenic chito-oligosaccharide analogues, p-nitrophenyl-beta-d-n,n'-diacetylchitobiose, p-nitrophenyl-n-acetyl-beta-d-glucosamine and p-nitrophenyl-beta-d-n,n',n"-triacetylchitotriose, (2) the release of reducing sugars from chitin and (3) the formation of clearing zones on ch ... | 1994 | 8157546 |
| community analysis of root- and tuber-associated bacteria in field-grown potato plants harboring different resistance levels against common scab. | eight genotypes of potato plants with different resistance levels against common scab were grown in a field infested with streptomyces turgidiscabies. dna was extracted from the roots, tubers, and rhizosphere soils of each of the eight genotypes at the flowering stage, and the quantity of s. turgidiscabies genomic dna was assessed by real-time pcr using a taqman probe. the results obtained showed that the different potato genotypes had significant impacts on the population levels of s. turgidisc ... | 2015 | 26657303 |
| a polyphasic reassessment of the genus paenibacillus, reclassification of bacillus lautus (nakamura 1984) as paenibacillus lautus comb. nov. and of bacillus peoriae (montefusco et al. 1993) as paenibacillus peoriae comb. nov., and emended descriptions of p. lautus and of p. peoriae. | seventy-seven strains representing 10 species in the paenibacillus polymyxa 16s rrna group and 3 other species that exhibit phenetic relatedness to members of this group, bacillus lautus, "bacillus longisporus," and bacillus peoriae, were characterized genotypically and phenotypically by performing an amplified ribosomal dna restriction analysis, a randomly amplified polymorphic dna analysis, a fatty acid methyl ester analysis, sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-c ... | 1996 | 8863428 |