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thermally caused filament formation in the psychrophile bacillus insolitus. 19734122689
protein synthesis by the psychophiles bacillus psychophilus and bacillus insolitus. 19724567973
diversity of bacteria and archaea from a landfill in chandigarh, india as revealed by culture-dependent and culture-independent molecular approaches.the bacterial community structure of a municipal landfill in chandigarh, india was analysed by culture-dependent as well as culture-independent molecular approaches, and archaeal structure by the latter method. samples were collected in two phases from the surface and a depth of 0.91 m in june, 2004 and from 0.91 m, 1.52 m and 1.68 m in may, 2005. after serial dilutions, samples were plated onto tryptic soy agar (tsa), plate count agar (pca), tryptic soy broth agar (tsba) and tsba100 (tsba dilut ...201223274043
psychrobacillus gen. nov. and proposal for reclassification of bacillus insolitus larkin & stokes, 1967, b. psychrotolerans abd-el rahman et al., 2002 and b. psychrodurans abd-el rahman et al., 2002 as psychrobacillus insolitus comb. nov., psychrobacillus psychrotolerans comb. nov. and psychrobacillus psychrodurans comb. nov.the taxonomic status of three bacillus species, bacillus insolitus, b. psychrodurans and b. psychrotolerans was reexamined using a polyphasic approach. in our analysis, these three bacillus species formed a cluster separate from other members of bacillus rrna group 2 [5] and from bacillus sensu stricto. these three species shared high 16s rrna gene sequence similarities between them (97.8-99.7%) and showed closest sequence similarity (95.3-96.3%) to paenisporosarcina quisquiliarum gen. nov., sp. ...201020650590
biosynthesis of branched-chain fatty acid in bacilli: fabd (malonyl-coa:acp transacylase) is not essential for in vitro biosynthesis of branched-chain fatty acids.it was found that the partially purified beta-ketoacyl-acp synthase of bacillus insolitus did not require the addition of fabd (malonyl-coa:acp transacylase, mat) for the activity assay. this study therefore examined the necessity of fabd protein for in vitro branched-chain fatty acid (bcfa) biosynthesis by crude fatty acid synthetases (fas) of bacilli. to discover the involvement of fabd in the bcfa biosynthesis, the protein was removed from the crude fas by immunoprecipitation. the his-tag fus ...200314586097
two novel psychrotolerant species, bacillus psychrotolerans sp. nov. and bacillus psychrodurans sp. nov., which contain ornithine in their cell walls.eleven psychrotolerant bacillus strains with ornithine as diamino acid in position 3 of the peptide side chain of the cell wall and a g+c range of 35.7-38.4 mol% were characterized taxonomically. dna-dna hybridization studies confirmed previously physiologically established groups. high dna-binding values (> 70%) were found within groups i a (consisting of the type strain of bacillus insolitus dsm 5(t) and bacillus insolitus dsm 2272), i b (consisting of isolates 3h1(t0, 71h1, 84e1, 87h2 and 4h2 ...200212508879
impact of cultivation on characterisation of species composition of soil bacterial communities.the species composition of culturable bacteria in scottish grassland soils was investigated using a combination of biolog and 16s rdna analysis for characterisation of isolates. the inclusion of a molecular approach allowed direct comparison of sequences from culturable bacteria with sequences obtained during analysis of dna extracted directly from the same soil samples. bacterial strains were isolated on pseudomonas isolation agar (pia), a selective medium, and on tryptone soya agar (tsa), a ge ...200111248388
removal of 2,4-dichlorophenol by suspended and immobilized bacillus insolitus.in this experiment, bacillus insolitus was isolated and selected from a mixed culture that have been acclimated to chlorophenols. decomposition of chlorophenolic compounds will be studied using this pure culture in both suspended and immobilized form. the results are: at lower initial concentrations of 2,4-dichlorophenol (10-50 mg/l), immobilized bacillus insolitus shows a higher removal of 2,4-dichlorophenol than bacillus insolitus in suspended growth. when the 2,4-dichlorophenol concentration ...200011057607
type selective inhibition of microbial fatty acid synthases by thiolactomycin.the antibiotic, thiolactomycin, is known to selectively inhibit the type ii straight-chain fatty acid synthase (monofunctional enzyme system, e.g. escherichia coli enzyme) but not type i straight-chain fatty acid synthase (multifunctional enzyme system, e.g. saccharomyces cerevisiae enzyme). we have studied the effect of thiolactomycin on the branched-chain fatty acid synthases from bacillus subtilis, bacillus cereus, and bacillus insolitus. fatty acid synthase from all three bacilli was not inh ...19938379811
fatty acid composition and primer specificity of de novo fatty acid synthetase in bacillus globispores, bacillus insolitus, and bacillus psychrophilus.the fatty acid compositions of three psychrophilic species of bacillus were determined by gas--liquid chromatography. the proportions of straight-chain fatty acids, branched-chain fatty acids, and unsaturated fatty acids were found to be 13.3, 86.7, and 26.1% of the total cellular fatty acids for bacillus globispores, 36.6, 63.4, and 25.1% for bacillus insolitus, and 6.9, 93.1, and 18.4% for bacillus psychrophilus, respectively. in all three organisms the de novo fatty acid synthetase specificit ...19836673817
reversal of filament formation in the psychrophile bacillus insolitus. 19744424021
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