| mechanism of chloramphenicol resistance in bacillus badius 211. | six strains of chloramphenicol (cm)-resistant endospore-forming bacteria, which can grow in the presence of 100 microgram/ml of cm, were isolated and identified as bacillus badius. mechanism of cm-resistance in one of the isolated strains, bacillus badius 211, was investigated. no inactivation of cm was demonstrated when the strain was grown in nutrient broth containing 100 microgram/ml of cm, as evidenced by paper-disc bioassay of cm in the growth medium. in accordance with this result, no cm a ... | 1978 | 721710 |
| isolation of three new antibiotics, thiocillins i, ii and iii, related to micrococcin p. studies on antibiotics from the genus bacillus. viii. | thiocillins i and ii were isolated from the culture broth of bacillus cereus g-15, and thiocillins ii and iii from that of bacillus badius ar-91. also, the former two were probably produced by bacillus megatherium i-13. these antibiotics active against gram-positive bacteria are soluble in a mixture of chloroform and methanol, show characteristic ultraviolet absorptions (maxima at ca. 275 nm and ca. 348 nm), and contain a high content of sulfur, as much as approximately 15%. they are related to ... | 1976 | 819410 |
| numerical taxonomy of bacillus isolated from orally administered drugs. | numerical taxonomy procedures were used to study 118 strains of bacillus isolated from non-sterile drugs prepared for oral administration. similarities between pairs of strains were calculated by the simple matching coefficient of sokal and michener (ssm). each strain was tested for 60 unit characters and three clusters were defined. the strains in each cluster presented a similarity level of at least 60%. cluster a comprised the strains identified as bacillus cereus (ssm = 93.13%), cluster b co ... | 1986 | 3096933 |
| phenylalanine dehydrogenase of bacillus badius. purification, characterization and gene cloning. | phenylalanine dehydrogenase produced by bacillus badius iam 11059 was purified from the crude extract of b. badius to homogeneity, as judged by disc gel electrophoresis. the enzyme has an isoelectric point of 3.5 and a relative molecular mass, mr, of 310,000-360,000. the enzyme is composed of identical subunits with an mr 41,000-42,000. the substrate specificity of the enzyme in the oxidative deamination reaction was high for l-phenylalanine, but rather low in the reductive amination reaction, w ... | 1987 | 3311741 |
| [relative appearance of quantities of carcinogenic hydrocarbons in pure cultures of bacillus badius as a function of the presence of some chemical compounds in culture media]. | | 1970 | 4986333 |
| [description of the type strain of bacillus badius]. | the type strain of bacillus badius (atcc 14574) was studied in detail. out of a total of 201 sources of carbon and energy tested, only 19 were used. these included mainly organic and amino acids. no carbohydrates were used. the g+c content of its dna was 47.5 mol %. the species b. badius therefore deserves to be included in approved lists of bacterial names. | 1984 | 6508070 |
| aerobic mesophilic and psychrotrophic sporeforming bacteria in buffalo milk. | seasonal variation of the population of aerobic sporeformers in raw milk was higher in summer than in other seasons. least variation was in fall, but variation in winter and spring was similar. aerobic mesophilic sporeformers in raw milk consisted mainly of bacillus subtilis (42.5%) and bacillus megaterium (34.8%), followed by bacillus circulans (4.9%), bacillus cereus (4.6%), bacillus pumilus (2.9%), bacillus polymyxa (2.8%), bacillus licheniformis (1.9%), bacillus badius (1.5%), bacillus brevi ... | 1983 | 6886164 |
| staphylococcus aureus has clustered trna genes. | the polymerase chain reaction (pcr) was used to detect large trna gene clusters in bacillus subtilis, bacillus badius, bacillus megaterium, lactobacillus brevis, lactobacillus casei, and staphylococcus aureus. the primers were based on conserved sequences of known gram-positive bacterial trna(arg) and trna(phe) genes. this pcr procedure detected an unusually large trna gene cluster in s. aureus. pcr-generated probes were used to identify a 4.5-kb ecori fragment that contained 27 trna genes immed ... | 1993 | 8349549 |
| nucleotide sequencing of phenylalanine dehydrogenase gene from bacillus badius iam 11059. | the nucleotide sequence of the pdh gene coding for phedh from bacillus badius iam 11059 was analyzed. the gene consists of an orf of 1140 nucleotides which specifies a protein of 380 codons. the primary structure of phedh is similar to phedh from b. sphaericus, phedh from themoactinomyces intermedius, and leucine dehydrogenase from b. stearothemophilus, etc. | 1995 | 8535000 |
| investigation of bacterial resistance to hatchery disinfectants. | three commercial chicken hatcheries were sampled for environmental bacteria. isolated bacteria were tested for resistance to commercial preparations of quaternary ammonia, phenolic, and glutaraldehyde liquid disinfectants. bacterial isolates were exposed to several disinfectant dilutions bracketing the dilutions recommended by the manufacturer for 5-, 10-, and 15-min exposure periods before subculturing to broth medium. approximately 8% of the isolates from two of three hatcheries were resistant ... | 1996 | 8883778 |
| analysis of the essential cell division gene ftsl of bacillus subtilis by mutagenesis and heterologous complementation. | the ftsl gene is required for the initiation of cell division in a broad range of bacteria. bacillus subtilis ftsl encodes a 13-kda protein with a membrane-spanning domain near its n terminus. the external c-terminal domain has features of an alpha-helical leucine zipper, which is likely to be involved in the heterodimerization with another division protein, divic. to determine what residues are important for ftsl function, we used both random and site-directed mutagenesis. unexpectedly, all che ... | 2000 | 10986263 |
| purification and characterization of nitrite-oxidizing enzyme from heterotrophic bacillus badius 1-73, with special concern to catalase. | nitrite-oxidizing enzyme i (niox i) was purified from a heterotrophic bacterium, bacillus badius i-73. the enzyme was a homotetramer of a heme-containing peptide, and was similar to catalases from various sources in its n-terminal amino acid sequence. the purified enzyme also catalyzed h2o2 degradation. the nitrite oxidation reaction required ascorbic acid and oxygen. successive h2o2 feeding could be substituted for ascorbic acid. these indicated that niox i is a catalase and nitrite was oxidize ... | 2000 | 11210148 |
| polyphasic identification of bacillus and brevibacillus strains from clinical, dairy and industrial specimens and proposal of brevibacillus invocatus sp. nov.. | thirty-three clinical, dairy and industrial isolates of aerobic endospore-forming bacteria which were unreactive in routine identification tests were characterized genotypically by using amplified rdna restriction analysis (ardra), 16s rdna sequencing and dna-dna reassociation, and phenotypically by using fatty acid methyl ester (fame) analysis, sds-page of whole-cell proteins, api biotype 100 assimilation tests and 16 other routine phenotypic tests. three isolates were identified as strains of ... | 2002 | 12054263 |
| single amino acid substitution in bacillus sphaericus phenylalanine dehydrogenase dramatically increases its discrimination between phenylalanine and tyrosine substrates. | homology-based modeling of phenylalanine dehydrogenases (phedhs) from various sources, using the structures of homologous enzymes clostridium symbiosum glutamate dehydrogenase and bacillus sphaericus leucine dehydrogenase as a guide, revealed that an asparagine residue at position 145 of b. sphaericus phedh was replaced by valine or alanine in phedhs from other sources. this difference was proposed to be the basis for the poor discrimination by the b. sphaericus enzyme between the substrates l-p ... | 2002 | 12234181 |
| rediscovery of bacillus badius batchelor. | | 1953 | 13034720 |
| increased conformational and thermal stability properties for phenylalanine dehydrogenase by chemical glycosidation with end-group activated dextran. | a mono-aminated dextran derivative was attached to bacillus badius phenylalanine dehydrogenase via a carbodiimide-catalyzed reaction. the optimum temperature for the conjugate was 10 degrees c higher than for native enzyme, and its thermostability was improved by 8 degrees c. the activation free energy of thermal inactivation at 45 degrees c was increased by 16.8 kj/mol. the improved conformational stability of the modified enzyme was confirmed by fluorescence spectroscopy. | 2005 | 16215831 |
| isolation, purification and properties of new restriction endonucleases from bacillus badius and bacillus lentus. | we tentatively named two enzymes as bbai and blei, which were isolated and purified from gram-positive mesophilic bacteria bacillus badius 1458 and bacillus lentus 1689 respectively, by ammonium sulphate precipitation, phosphocellulose and heparin-sepharose column chromatography. sds-page protein profiles for bbai and blei showed denatured molecular weights of 52 and 48 kda, respectively. bbai hydrolyzed puc18 dna into 1900 and 700 bp, pbr322 dna into two fragments of 2800 and 1500 bp and phix17 ... | 2007 | 16644193 |
| application of cross-linked enzyme aggregates of bacillus badius penicillin g acylase for the production of 6-aminopenicillanic acid. | optimization of 6-aminopenicillanic acid (6-apa) production using cross-linked enzyme aggregates (clea) of bacillus badius penicillin g acylase (pac). | 2007 | 17209813 |
| purification of recombinant phenylalanine dehydrogenase by partitioning in aqueous two-phase systems. | this study presents the partitioning and purification of recombinant bacillus badius phenylalanine dehydrogenase (phedh) in aqueous two-phase systems (atps) composed of polyethylene glycol 6000 (peg-6000) and ammonium sulfate. a single-step operation of atps was developed for extraction and purification of recombinant phedh from e. coli bl21 (de3). the influence of system parameters including; peg molecular weight and concentration, ph, (nh(4))(2)so(4) concentration and nacl salt addition on enz ... | 2007 | 17537685 |
| molecular cloning and characterization of thermostable beta-lactam acylase with broad substrate specificity from bacillus badius. | the gene (pac) encoding beta-lactam acylase from bacillus badius was cloned and expressed in escherichia coli. the pac gene was identified by polymerase chain reaction (pcr) using degenerated primers, on the basis of conserved amino acid residues. by using single specific primer pcr (ssp-pcr) and direct genome sequencing, a complete pac gene with its promoter region was obtained. the orf consisted of 2415 bp and the deduced amino acid sequence indicated that the enzyme is synthesized as a prepro ... | 2007 | 17609162 |
| bioluminescent flow sensor for l-phenylalanine determination in serum. | a highly sensitive and rapid bioluminescent flow sensor was developed for the determination of the content of l-phenylalanine (phe) in serum by monitoring the reduced form of nicotinamide adenine dinucleotide (nadh), produced by immobilized phenylalanine dehydrogenase (phedh), with bacterial bioluminescent enzymes immobilized on a separate nylon coil. the l-phedhs extracted from bacillus badius, bacillus sphaericus and rhodococcus sp. m 4 were investigated and the performances of the three immob ... | 1993 | 18965647 |
| escravos light crude oil degrading potentials of axenic and mixed bacterial cultures. | we assessed the relationship between growth profile and the extent of biodegradation of escravos light crude oil by axenic and mixed bacteria cultures in a shake flask. eleven petroleum-degrading bacteria were isolated by enrichment from oil-contaminated soils including, pseudomonas effusa, pseudomonas fluorescens, pseudomonas cruciviae, arthrobacter tumescens, pseudomonas species, pseudomonas tralucida, alcaligenes metacaligenes, micrococcus colpogenes, bacillus badius, nocardia paraffinae and ... | 2008 | 19029769 |
| discrimination of aliphatic substrates by a single amino acid substitution in bacillus badius and bacillus sphaericus phenylalanine dehydrogenases. | replacement of glycine by serine at positions 123 and 124 of phenylalanine dehydrogenases from bacillus badius and bacillus sphaericus respectively strikingly decreased enzyme activity toward aromatic amino acids and resulted in an elevation of relative activity toward aliphatic amino acids. the mutant from b. badius preferentially dehydrogenated branched-chain amino acids, while that from b. sphaericus acted on amino acids with straight-chain amino acids. | 2009 | 19270409 |
| investigation of chromatography and polymer/salt aqueous two-phase processes for downstream processing development of recombinant phenylalanine dehydrogenase. | this work presents a comprehensive study between the polymer/salt aqueous two-phase systems (atps) and chromatography process for downstream processing of recombinant bacillus badius phenylalanine dehydrogenase (phedh). first, the partitioning behavior of recombinant phedh in polyethylene glycol (peg)/k2hpo4 atps was examined. for comparative purpose, a classical chromatographic protocol was performed as well. investigation of chromatography and atps procedures revealed that the atps comprising ... | 2010 | 19495799 |
| bacillus acidiproducens sp. nov., vineyard soil isolates that produce lactic acid. | two novel spore-forming lactic acid bacteria, strains sl213t and sl1213, were isolated from vineyard soils in korea. cells of both isolates were rod-shaped bacilli and contained subterminal, ellipsoidal spores. strains were facultatively anaerobic, catalase-positive, oxidase-negative and motile with single flagella. meso-diaminopimelic acid, glucose and galactose were detected in whole-cell hydrolysates. major fatty acids found in the strains were anteiso-c15:0, iso-c15:0, iso-c16:0, c16:0 and a ... | 2009 | 19605722 |
| biosorption of lead using bacillus badius ak strain isolated from compost of green waste (water hyacinth). | the bacterial strain bacillus badius ak isolated from water hyacinth compost was investigated for biosorption characteristics in pb(ii) removal. batch mode experiments depicted the optimum conditions for biosorption as ph at 4, the temperature of 30°c, 150 rpm of the rotational speed at biomass concentration of 20 ml with 1.7 × 10(16) colony forming unit per milliliter (cfu/ml) value, at 100-150 mg/l concentration of pb(ii). the bacterial biomass was used in its native and non-pretreated state, ... | 2017 | 28278101 |
| biosorption of pb(ii) by bacillus badius ak strain originating from rotary drum compost of water hyacinth. | the presence of heavy metals in the environment due to industrial activities is of serious concern because of their toxic behaviour towards humans and other forms of life. biosorption of pb(ii) using dry bacterial biomass of bacillus badius ak, previously isolated from water hyacinth compost, has been undertaken in batch system. the optimum conditions of biosorption were determined by investigating the initial ph, contact time, initial biomass dosage at constant temperature of 40 °c, initial met ... | 2017 | 28272037 |
| bacillus encimensis sp. nov. isolated from marine sediment. | a gram-stain-positive, rod-shaped, endospore-forming, aerobic bacterium designated sgd-v-25(t) was isolated from veraval sediment sample, india. strain sgd-v-25(t) was capable of growing at 25-50 °c (optimum 37 °c), ph 6-12 (optimum ph 7.0) and with 0-5% (w/v) nacl. the taxonomic position of this strain was deduced using a polyphasic approach and the 16s rrna gene sequence analysis showed that the isolate belongs to the phylum firmicutes , forming the cluster with bacillus badius mtcc 1548(t), w ... | 2015 | 25678682 |
| biological real-time reaction calorimeter studies for the production of penicillin g acylase from bacillus badius. | penicillin g acylase (pga) is a commercially important enzyme that cleaves penicillin g to 6-amino penicillanic acid (6-apa) and phenyl acetic acid (paa). the strain bacillus badius has been identified as potential producer of pga. a detailed calorimetric investigation on pga production was carried out to enable generation of thermokinetic data possible for commercial application. reaction calorimetric studies coupled with respirometric studies suggested that enzyme activity of the species b. ba ... | 2014 | 24566926 |
| neutralization of the antimicrobial effect of glyphosate by humic acid in vitro. | in the present study, the neutralization ability of the antimicrobial effect of glyphosate by different humic acids was investigated. the minimal inhibitory concentrations of glyphosate for different bacteria such as bacillus badius, bifidobacterium adolescentis, escherichia coli, e. coli 1917 strain nissle, enterococcus faecalis, enterococcus faecium, salmonella enteritidis and salmonella typhimurium were determined in the presence or absence of different concentrations of humic acid (0.25, 0.5 ... | 2014 | 24268342 |
| strategies for enhancing the production of penicillin g acylase from bacillus badius: influence of phenyl acetic acid dosage. | bacillus badius isolated from soil has been identified as potential producer of penicillin g acylase (pga). in the present study, batch experiments performed at optimized inoculum size, temperature, ph, and agitation yielded a maximum pga of 9.5 u/ml in shake flask. the experiments conducted in bioreactor with different oxygen flow rates revealed that 0.66 vvm oxygen flow rate could be sufficient for the maximum pga activity of 12.7 u/ml. from a detailed investigation on the strategies of the ad ... | 2013 | 23949729 |
| bacillus thermotolerans sp. nov., a thermophilic bacterium capable of reducing humus. | a novel thermotolerant bacterium, designated sgz-8(t), was isolated from a compost sample. cells were non-motile, endospore-forming, gram-staining positive, oxidase-negative and catalase-positive. the isolate was able to grow at 20-65 °c (optimum 50 °c) and ph 6.0-9.0 (optimum 6.5-7.0), and tolerate up to 9.0 % nacl (w/v) under aerobic conditions. anaerobic growth occurred with anthraquinone-2,6-disulphonate (aqds), fumarate and no3(-) as electron acceptors. phylogenetic analysis based on the16s ... | 2013 | 23625259 |
| the effect of glyphosate on potential pathogens and beneficial members of poultry microbiota in vitro. | the use of glyphosate modifies the environment which stresses the living microorganisms. the aim of the present study was to determine the real impact of glyphosate on potential pathogens and beneficial members of poultry microbiota in vitro. the presented results evidence that the highly pathogenic bacteria as salmonella entritidis, salmonella gallinarum, salmonella typhimurium, clostridium perfringens and clostridium botulinum are highly resistant to glyphosate. however, most of beneficial bac ... | 2013 | 23224412 |
| domibacillus robiginosus gen. nov., sp. nov., isolated from a pharmaceutical clean room. | a novel red-pigmented bacterial strain, designated ws 4628(t), was isolated from a pharmaceutical clean room of a vaccine-producing company and was investigated in a taxonomic study using a polyphasic approach. the strain was gram-stain-positive, strictly aerobic, motile, catalase-positive and produced spherical to slightly ellipsoidal endospores in rods. the genomic dna g+c content was 44.1 mol%. the major fatty acids were anteiso-c15:0, iso-c15:0 and anteiso-c17:0 and the predominant quinone w ... | 2013 | 23064349 |
| biodegradation of shrimp processing bio-waste and concomitant production of chitinase enzyme and n-acetyl-d-glucosamine by marine bacteria: production and process optimization. | a total of 250 chitinolytic bacteria from 68 different marine samples were screened employing enrichment method that utilized native chitin as the sole carbon source. after thorough screening, five bacteria were selected as potential cultures and identified as; stenotrophomonas sp. (cfr221 m), vibrio sp. (cfr173 m), phyllobacteriaceae sp. (cfr16 m), bacillus badius (cfr198 m) and bacillus sp. (cfr188 m). all five strains produced extracellular chitinase and glcnac in ssf using shrimp bio-waste. ... | 2012 | 22806736 |
| bacillus wudalianchiensis sp. nov., isolated from grass soils of the wudalianchi scenic area. | a gram-stain-positive, rod-shaped, endospore-forming, aerobic bacterium, designated fjat-27215t, was isolated from grass soil collected from wudalianchi in the heilongjiang province of china. growth was observed at 10-60 °c (optimum 30 °c), in 0 and 3.0 % nacl (optimum 0 %) and at ph 5.0-10.0 (optimum 7.0), respectively. the cell-wall peptidoglycan contained meso-diaminopimelic acid and the isoprenoid quinone was mk7. the main fatty acids were iso-c15 : 0, anteiso-c15 : 0, anteiso-c17 : 0, and i ... | 2017 | 28820124 |