| evidence of the involvement of a 50s ribosomal protein in several active sites. | the functional role of the bacillus stearothermophilus 50s ribosomal protein b-l3 (probably homologous to the escherichia coli protein l2) was examined by chemical modification. the complex b-l3-23s rna was photooxidized in the presence of rose bengal and the modified protein incorporated by reconstitution into 50s ribosomal subunits containing all other unmodified components. particles containing photooxidized b-l3 are defective in several functional assays, including (1) poly(u)-directed poly( ... | 1975 | 52 |
| succinylation of glyceraldehyde-3-phosphate dehydrogenase from bacillus stearothermophilus. a reactive threonine residue in the apoenzyme. | 1. glyceraldehyde-3-phosphate dehydrogenase from bacillus stearothermophilus can be extensively succinylated in the presence of substrates and coenzyme without appreciable loss of activity. 2. the apoenzyme in the absence of substrates is rapidly inhibited by small amounts of succinic anhydride. nad+, glyceraldehyde-3-phosphate and inorganic phosphate all afford protection from inhibition, and inhibition is slowly reversed in the presence of pyrophosphate at ph 8.5. 3. kinetic and spectral studi ... | 1976 | 4305 |
| glutamine synthetase of bacillus stearothermophilus. regulation, site interactions, and functional information. | the action of various feedback modifiers on bacillus stearothermophilus glutamine synthetase has been investigated by initial velocity kinetics, using the mn2+-stimulated biosynthetic assay at 55 degrees c. the most potent inhibitors, used singly, are amp, l-glutamine, and l-alanine. other modifiers of significance include glycine, ctp, l-histidine, glucosamine 6-phosphate, and gdp. marked synergism of action is observed for amp in the presence of l-glutamine, l-histidine, adp, or glucosamine 6- ... | 1976 | 5112 |
| purification and characterization of inorganic pyrophosphatase from bacillus stearothermophilus. | inorganic pyrophosphatase ec 3.6.1.1 was purified from bacillus stearothermophilus to a homogeneous state both ultracentrifugally and electrophoretically. ultracentrifugal analysis revealed that the molecular weight of the enzyme is 122,000 and the sedimentation coefficient (s0.34%/20, w) is 5.2s. the enzyme molecule in 0.1% sodium dodecylsulfate solution containing 1 mm 2-mercaptoethanol had an estimated molecular weight of 70,000 on the basis of sds-polyacrylamide gel electrophoresis results, ... | 1975 | 5398 |
| the modification of sulfhydryl groups of glutamine synthetase from bacillus stearothermophilus with 5, 5'-dithiobis(2-nitrobenzoic acid). | the sh groups of glutamine synthetase ec 6.3.1.2 from bacillus stearothermophilus were modified with 5, 5'-dithiobis(2-nitrobenzoic acid) in order to determine the number of sh groups in the molecule as well as the effect of the modification on the enzyme activity. three sh groups per subunit were detected after complete denaturation of the enzyme with 6 m urea, one of which was essential for the enzyme activity in view of its reactivity with 5, 5'-dithiobis(2-nitrobenzoic acid) on addition of ... | 1975 | 5420 |
| beta-galactosidase from bacillus stearothermophilus. | several strains of thermophilic aerobic spore-forming bacilli synthesize beta-galactosidase (ec 3.2.1.23) constitutively. the constitutivity is apparently not the result of a temperature-sensitive repressor. the beta-galactosidase from one strain, investigated in cell-free extracts, has a ph optimum between 6.0 and 6.4 and a very sharp ph dependence on the acid side of its optimum. the optimum temperature for this enzyme is 65 degrees c and the arrhenius activation energy is about 24 kcal/mol be ... | 1976 | 6142 |
| thermothrix thioparus gen. et sp. nov. a facultatively anaerobic facultative chemolithotroph living at neutral ph and high temperature. | thermothrix thioparus gen. et ep. nov. occurs naturally in a new mexico hot spring at a temperature of 74 degrees c, a ph of 7.0, and a hs- concentration of 1 mg/litre. the organism is gram-negative, non-motile, 0.5-1.0 x 3-20 mum, and forms cell chains up to 1 cm in length. the resulting filaments do not possess a sheath. sulfur is deposited extracellularly. the organism was isolated using an autotrophic medium with hs- as the energy source and no3- as the terminal electron acceptor. anaerobica ... | 1976 | 10063 |
| some catalytic and molecular properties of threonine deaminase from bacillus stearothermophilus. | threonine deaminase ec 4.2.1.16 was highly purified from bacillus stearothermophilus. the enzyme exhibited maximum activity at 65 degrees and at ph 9.2--9.6. it was inactivated on dilution and on storage at 4 degrees, but was protected by egg albumin. the enzyme was labile at 65 degrees, but became stable in the presence of egg albumin and isoleucine at ph 7.0. the substrate saturation curve for the enzyme reaction at 40 or 65 degrees was hyperbolic, but in the presence of isoleucine, the curve ... | 1976 | 10288 |
| isolation and characterization of a bacteriocin produced by bacillus stearothermophilus strain nu-10. | broth-grown cultures of bacillus stearothermophilus strain nu-10 produce a bacteriocin which exerts lethal activity on other strains of the bacterium. optimal production occurs during late maximum stationary phase of growth, at neutral ph, and 55-65 degrees c. the bacteriocin can be substantially purified by a combination of precipitations, centrifugations, and gel filtrations. the thermocin is composed of protein and carbohydrate. it is partially destroyed by proteolytic enzymes but is resistan ... | 1976 | 12863 |
| superoxide dismutase from thermus aquaticus. isolation and characterisation of manganese and apo enzymes. | superoxide dismutase has been isolated and characterised from the extreme thermophile thermus aquaticus. the pure enzyme is a reddish-purple manganese-containing protein with a molecular weight of approximately 80000 +/- 5000. combination of gel electrophoresis in dodecylsulphate and amino acid analysis shows that it is composed of four identical subunit polypeptide chains consisting of approximately 186 amino acids. the tetrameric protein contains two atoms of manganese. a stable manganese-free ... | 1977 | 14828 |
| dtnb modification of sh groups of isocitrate dehydrogenase from bacillus stearothermophilus purified by affinity chromatography. | a new method of affinity chromatography using blue dextran-sepharose 4b resin was established to purify nadp+-dependent isocitrate dehydrogenase ec 1.1.1.42 from bacillus stearothermophilus in high yield. the purified preparation was found to be homogeneous on disc gel electrophoresis. the sh groups of the enzyme were modified with 5,5'-dithiobis-(2-nitrobenzoic acid) (dtnb) to determine the number of sh groups per molecule and their contribution to the enzyme activity. one sh group was titrated ... | 1977 | 14937 |
| a pulse-radiolysis study of the manganese-containing superoxide dismutase from bacillus stearothermophilus. | in the preceding paper the mechanism of catalysis of the manganese-containing superoxide dismutase from bacillus stearothermophilus was shown to involve a 'fast cycle' and a 'slow cycle' mcadam, fox, lavelle & fielden, 1977 (biochem. j. 165, 71-79). further properties of the enzyme was considered in the present paper. pulse-radiolysis studies, under conditions of low substrate concentration to (i.e. when the fast cycle predominates), showed that enzyme activity decreases as ph increases (6.5-10. ... | 1977 | 19013 |
| fluorescent labelling of 6-phosphogluconate dehydrogenase from bacillus stearothermophilus. | the thermophilic 6-phosphogluconate dehydrogenase from bacillus stearothermophilus was inhibited upon specific modification of the -sh group of cysteine residues by 7-chloro-4-nitrobenzo-2-oxa-1, 3-diazole (nbd-cl) at ph 7.0. by using 20-100-fold molar excess of nbd-cl the reaction occurs slowly at ph 7.0 as a first order process. partial protection from inactivation was observed when the substrate 6-phosphogluconate or the coenzyme nadp was added to the reaction mixture. complete inactivation w ... | 1977 | 19368 |
| the identification of lipid acceptor and the biosynthesis of lipid-linked glucose in bacillus stearothermophilus. | | 1977 | 20047 |
| affinity chromatography of aminoacyl-transfer ribonucleic acid synthetases. cognate transfer ribonucleic acid as a ligand. | the use of trna affinity columns for the purification of aminoacyl-trna synthetases was investigated. a purification method for valyl-trna synthetase from bacillus stearothermophilus is described that uses two affinity columns, one containing the pure cognate trna, and the other containing all trna species except the cognate trna. a method for the rapid preparation of the two columns was developed, which does not require prior isolation of cognate trna but makes use of the ability of the target ... | 1977 | 23108 |
| purification and some properties of riboflavin synthetase from bacillus stearothermophilus atcc 8005. | a riboflavin synthetase was purified 51-fold from a thermophilic organism, bacillus stearothermophilus atcc 8005, that grew at 40 to 72 degrees c. some of the properties of the enzyme are: (i) its temperature optimum was 95 degrees c, and the activity was negligible below 40 degrees c; (ii) the arrhenius plot of the initial reaction rates was concave upward, with a break at 65 degrees c, and the apparent activation energies below and above 65 degrees c were 4.2 x 10(4) and 6.7 x 10(4) j/mol, res ... | 1978 | 25043 |
| purification and properties of acetate kinase from bacillus stearothermophilus. | 1. acetate kinase ec 2.7.2.1 from an thermophile, b. stearothermophilus, was purified and crystalized. 2. this enzyme was shown to be a tetramer of identical subunits which had a molecular weight of about 40,000. amino acid analysis showed no sh group. by analyzing the cd spectrum it was deduced that this enzyme is composed of 36% beta-structure, 21% alpha-helix and 43% unordered structure. 3. this enzyme shared many common enzymatic properties with the counterpart from mesophiles, i.e. ph optim ... | 1978 | 29037 |
| [dna synthesis and degradation in the cells of bacillus stearothermophilus]. | atp-dependent and atp-independent synthesis of dna was studied on nucleotide-permeable cells of bacillus stearothermophilus. the effect of temperature, ph, ionic strength, and bivalent metal ions on both types of dna synthesis was investigated as well as their susceptibility to an sh-blocking agent. the level of atp-independent synthesis of dna in the cells of bac. stearothermophilus was found to be ten times higher than that in e. coli. in the semipermeable cells of bac. stearothermophilus, 90% ... | 1978 | 30883 |
| subunit structure of acetate kinase from bacillus stearothermophilus as studied by hybridization and cross-linking experiments. | | 1978 | 32170 |
| chemical modification of epsilon-amino groups in glutamine synthetase from bacillus stearothermophilus with ethyl acetimidate. | the activity of glutamine synthetase [ec 6.3.2.1] from bacillus stearothermophilus decreased slightly on modification with ethyl acetimidate. acetamidination of 25--26 of the 2 epsilon-amino groups/subunit of the enzyme affected the maximum velocity, but not the michaelis constant. the thermostability of the enzyme was considerably increased on acetamidination. acetamidination of the enzyme did not affect the circular dichroism, the tryptophan fluorescence or the quenching effects of ki and acry ... | 1979 | 33165 |
| cross-linking with diimidates of glutamine synthetase from bacillus stearothermophilus. | glutamine synthetase [ec 6.3.2.1] from bacillus stearothermophilus was modified with diethyl malonimidate (dem), dimethyl adipimidate (dma), and dimethyl suberimidate (dms). dma modified most epsilon-amino groups. on modification with dma, formation of 3 to 4 cross-links/subunit resulted in a large increase in thermostability. the activity, allosteric properties and fluorescence spectrum of the enzyme were not changed on cross-linking. the sds-polyacrylamide gel electrophoretic profiles of dem-, ... | 1979 | 39071 |
| thermostable aminopeptidase from local isolate of bacillus stearothermophilus. | two of 63 isolates from different sources were very active in producing thermostable aminopeptidase. comparing the two isolates, rr-2247 gave the highest enzyme activity and was identified as bacillus stearothermophilus. the enzyme was isolated from the cells by a sonic vibration for 3 minutes at 20 kc/s. the enzyme shows optimum activity over the ph range of 7.5-8.0 at 65 degrees c. the apparent temperature optimum was about 70 degrees c. addition of 0.0001 m co2+ ions stabilized the enzyme in ... | 1979 | 44475 |
| the primary structure of trna phe from bacillus stearothermophilus and its analogy with other trna's from different origins. | | 1975 | 58588 |
| isoleucyl-trna synthetase from bacillus stearothermophilus: specificity of the aminoacylation reaction for aminoacid and trna. | | 1975 | 58626 |
| [proceedings: structure of arginyl-t-rna synthetase sub-units from bacillus stearothermophilus]. | | 1976 | 60939 |
| proceedings: the primary structure of trna2val from bacillus stearothermophilus and its analogy with trnaval species from different origins. | | 1976 | 60956 |
| studies on the negative circular dichroic bands around 297 nm of ribosomes from bacterial cells. | the small negative cd bands around 297 nm of isolated 30-s and 50-s ribosomal subunits were precisely measured for three bacteria, bacillus stearothermophilus, bacillus subtilis and escherichia coli q 13. the intensities of the negative cd bands of 30-s subunits were always much greater than those of 50-s subunits irrespective of the bacterial strains, which may be related to the difference in comformations of rrnas and proteins in the complexes between these subribosomal particles. the dissocia ... | 1978 | 96858 |
| isolation of a pyrophosphorylase from bacillus subtilis and bacillus stearothermophilus that specifically degrades guanosine 3'-diphosphate 5'-diphosphate. | | 1978 | 103752 |
| mechanism of penicillin action: penicillin and substrate bind covalently to the same active site serine in two bacterial d-alanine carboxypeptidases. | it has been hypothesized that penicillin acts as a structural analog of the acyl-d-alanyl-d-alanine terminus of nascent bacterial cell wall and that it consequently binds to and acylates the active site of the enzyme(s) that crosslinks the cell wall to form an inactive penicilloyl enzyme [tipper, d.j. & strominger, j.l. (1965) proc. natl. acad. sci. usa 64, 1133-1138]. this study directly proves that penicillin acylates the active site of two penicillin-sensitive enzymes, d-alanine carboxypeptid ... | 1979 | 111240 |
| structure and function of l-lactate dehydrogenases from thermophilic and mesophilic bacteria. i) isolation and characterization of lactate dehydrogenases from thermophilic and mesophilic bacilli. | lactate dehydrogenases from thermophilic bacilli (bacillus stearothermophilus, bacillus caldotenax) and from mesophilic bacilli (bacillus x1, bacillus subtilis) have been isolated by a two-step purification procedure. only one type (ldh-p4) composed of four identical subunits (mr 34 000 or 36 000) was found in each bacillus. the tetrameric enzymes were characterized with respect to thermostability, ph and temperature dependence of the pyruvate reduction and the l-lactate oxidation, substrate spe ... | 1979 | 114469 |
| effect of adp on atpase from a strain of bacillus stearothermophilus. | bacillus stearothermophilus atcc 12016 was unable to grow at temperatures below 40 degrees c. on incubating the bacteria at the temperatures, atp in cells disappeared, adp was accumulated and atpase (ec 3.6.1.3) was inactivated. when the purified atpase was incubated at the temperatures for 1 h with 0.17 mm adp in the presence of mgcl2, the enzyme was completely inactivated. the inactivated enzyme was reactivated on dilution or dialysis or on warming at 65 degrees c. during the incubation of the ... | 1977 | 137750 |
| escherichia coli 5s rna binding proteins l18 and l25 interact with 5.8s rna but not with 5s rna from yeast ribosomes. | reconstitution experiments showed that the two escherichia coli 5s rna binding proteins l18 and l25 form a specific complex with yeast 5.8s rna and not with yeast 5s rna. the yeast 5.8s rna-e. coli protein complex was found to exhibit atpase and gtpase activities that had previously been observed for the e. coli 5s rna-protein complex. the tetranucleotide upupcpg, which is an analog of the trna fragment tpsipcpg, interacted strongly with 5s rna-protein complexes from e. coli and bacillus stearot ... | 1977 | 142985 |
| phosphofructokinase from bacillus stearothermophilus [proceedings]. | | 1977 | 143385 |
| the 5-s rna . protein complex from an extreme halophile, halobacterium cutirubrum. purification and characterization. | a 5-s rna . protein complex has been isolated from the 50-s ribosomal subunit of an extreme halophile, halobacterium cutirubrum. the 50-s ribosomal subunit from the extreme halophile requires 3.4 m k+ and 100 mm mg2+ for stability. however, if the high k+ concentration is maintained but the mg2+ concentration lowered to 0.3 mm, the 5-s rna . protein complex is selectively extracted from the subunit. after being purified on an agarose 0.5-m column the complex had a molecular weight of about 80000 ... | 1978 | 152199 |
| structure and control of phosphofructokinase from bacillus stearothermophilus. | the allosteric enzyme phosphofructokinase binds its substrate fructose-6-phosphate between two subunits of the tetramer, and allosteric effectors between another pair of subunits. the effector binding site accommodates both the activator and the inhibitor. the substrate cooperativity and allosteric control are mediated by these ligand bridges between subunits. | 1979 | 156307 |
| protein-associated lipid of bacillus stearothermophilus. | the composition and patterns of metabolism of phospholipids isolated as part of a lipid-depleted membrane fragment (ldm fragment) and associated with the membrane adenosine triphosphatase complex have been compared with those of the bulk membrane phospholipid. the bulk lipid was extracted from washed membranes with sodium cholate. the ldm fragments, which contained a portion of the electron transport system and the membrane adenosine triphosphatase complex, were purified by chromatography with s ... | 1979 | 159285 |
| ligand binding and enzymic catalysis coupled through subunits in tyrosyl-trna synthetase. | the interaction of the tyrosyl-trna synthetase from bacillus stearothermophilus with its substrates in the aminoacyl adenylation reaction has been studied by stopped-flow fluorescence. the observed changes have been assigned to their chemical and physical processes by comparison with equilibrium dialysis, pyrophosphate exchange kinetics and rapid quenching and sampling techniques to give the rate constants for ligand binding, the formation of tyrosyl adenylate, and the reverse reaction. the stoi ... | 1975 | 162826 |
| reconstitution of bacillus stearothermophilus 50 s ribosomal subunits from purified molecular components. | bacillus stearothermophilus 50 s ribosomal subunits have been reconstituted from a mixture of purified rna and protein components. the protein fraction of 50 s subunits was separated into 27 components by a combination of various methods including ion exchange and gel filtration chromatography. the individual proteins showed single bands in a variety of polyacrylamide gel electrophoresis systems, and nearly all showed single spots on two-dimensional polyacrylamide gels. the molecular weights of ... | 1976 | 172511 |
| poly(glucosylglycerol phosphate) teichoic acid in the walls of bacillus stearothermophilus b65. | 1. walls of bacillus stearothermophilus b65 contain a glycerol teichoic acid in which repeating structures consisting of 1-o-alpha-d-glucopyranosylglycerol phosphate are held together by phosphodiester linkage between the glycerol and glucose moieties of adjacent units. 2. the walls are not agglutinated on incubation with concanavalin a, nor does the isolated teichoic acid form a precipitate with this lectin. 3. no evidence was obtained of the presence of the glucosylated (1 leads to 2)-poly(gly ... | 1975 | 174549 |
| physicochemical characterization of the four-iron-four-sulphide ferredoxin from bacillus stearothermophilus. | 1. a stable ferredoxin was prepared from bacillus stearothermophilus and purified by chromatography on deae-cellulose and by electrophoresis. 2. the minimum molecular weight determined from the amino acid composition was about 7900 and this was in reasonable agreement with a value of 8500 determined by polyacrylamide-gel electrophoresis. the ferredoxin contained four iron atoms and four labile sulphide groups per molecule. 3. the optical absorption, optical-rotatory-dispersion and circular-dichr ... | 1975 | 174558 |
| effect of temperature on the viability of bacillus stearothermophilus. | one of the obligate thermophilic bacteria, bacillus stearothermophilus, was unable to grow at temperatures below 35 degrees c. about 80% of the population in the bacterial culture died at the temperatures, and the same extent of loss in either of the activities of oxygen consumption or synthesis of protein or nucleic acid of the organisms was observed. with the progress of death of the organisms, reduced nicotinamide-adenine dinucleotide came to be oxidized by the organisms, enzymes such as fruc ... | 1976 | 175753 |
| the binding of nicotinamide-adenine dimucleotide to glyceraldehyde 3-phosphate dehydrogenase from bacillus stearothermophilus. | the binding of nad+ to glyceraldehyde 3-phosphate dehydrogenase (ec 1.2.1.12) from bacillus stearothermophilus has been studied by measurement of protein fluorescence quenching. slight negative co-operativity was observed in the binding of the third and fourth coenzyme molecules to the tetrameric enzyme. the first two coenzyme molecules were tightly bound. in this respect the enzyme resembles that from sturgeon muscle rather than that from yeast. | 1975 | 175789 |
| oxygen induced transformation of the nadh-nitrate oxidoreductase from bacillus stearothermophilus. | | 1976 | 181264 |
| iodination of glyceraldehyde 3-phosphate dehydrogenase from bacillus stearothermophilus. | the reaction of iodine with glyceraldehyde 3-phosphate dehydrogenase from bacillus stearothermophilus was investigated. the active-site thiol group of the cysteine residue homologous with cysteine-149 in the pig muscle enzyme was protected by reaction with tetrathionate. the apoenzyme was readily inhibited by ki3 solution at ph8, but the coenzyme, nad+, protected the enzyme against inhibition and decreased the extent of iodination. at ph 9.5, ready inhibition of both apo- and holo-enzyme was obs ... | 1976 | 182130 |
| enzyme hyperspecificity. rejection of threonine by the valyl-trna synthetase by misacylation and hydrolytic editing. | valyl-trna synthetase from bacillus stearothermophilus activates thereonine and forms a 1:1 complex with threonyl adenylate, but it does not catalyze the net formation of threonyl-trnaval at ph 7.78 and 25 degrees c in the quenched flow apparatus it decomposes at a rate constant of 36s-1. during this process there is a transient formation of thr-trnaval reaching a maximum at 25 ms and rapidly falling to zero after 150 ms. at the peak, 22% of the (14c) threonine from the complex is present as ( ... | 1976 | 182209 |
| the relationship between environmental temperature, cell growth and the fluidity and physical state of the membrane lipids in bacillus stearothermophilus. | a definite and characteristic relationship exists between growth temperature, fatty acid composition and the fluidity and physical state of the membrane lipids in wild type bacillus stearothermophilus. as the environmental temperature is increased, the proportion of saturated fatty acids found in the membrane lipids is also markedly increased with a concomitant decrease in the proportion of unsaturated and branched chain fatty acids. the temperature range over which the gel to liquid-crystalline ... | 1976 | 183821 |
| [microbial resistance to formaldehyde. i. comparative quantitative studies in some selected species of vegetative bacteria, bacterial spores, fungi, bacteriophages and viruses]. | the resistence of different microorganisms to formaldehyde was determined. as test objects served gram-negative and gram-positive vegetative germs (klebsiella pneumoniae, pseudomonas aeruginosa, salmonella paratyphi-b, staphylococcus aureus, streptococcus faecalis), bacterial spores (bacillus cereus, bacillus pumilus, bacillus stearothermophilus, bacillus subtilis), fungi (aspergillus niger, candida albicans), bacteriophages (escherichia coli phages, t1, t2, t3), and viruses (adenovirus, poliomy ... | 1976 | 190825 |
| sequence and structure of d-glyceraldehyde 3-phosphate dehydrogenase from bacillus stearothermophilus. | the glyceraldehyde 3-phosphate dehydogenase holoenzyme of bacillus stearothermophilus possesses precise 222 symmetry: in this respect it differs from the reported structure of the lobster muscle enzyme. pairs of active sites are linked through a flexible polypeptide loop which probably mediates the structural changes giving rise to cooperative effects. three additional salt bridges made by each subunit to others would make a major contribution to thermostability of the tetramer. | 1977 | 193030 |
| uridine-cytidine kinase from novikoff ascites rat tumor and bacillus stearothermophilus. | | 1978 | 211377 |
| purification of alcohol dehydrogenase from bacillus stearothermophilus by affinity chromatography. | | 1978 | 212307 |
| generation of superoxide anions and hydrogen peroxide from beta-lapachone in bacteria. | beta-lapachone markedly increased the generation of superoxide anions and hydrogen peroxide by subcellular membranes of bacillus subtilis and bacillus stearothermophilus. peroxide generation by beta-lapachone was parallel to the inhibition of growth in both microorganisms. | 1978 | 214029 |
| a ribosome-independent stringent factor from bacillus stearothermophilus and a low molecular weight substance inhibitory to its activity. | | 1979 | 216581 |
| studies on the allosteric nature of acetate kinase from bacillus stearothermophilus. | fructose 1,6-bisphosphate (fbp) stimulates the reaction of bacillus stearothermophilus acetate kinase (ak). fbp changes the reaction curve for atp from a sigmoidal type to a michaelis-menten one. the binding of fbp to ak was studied by an equilibrium dialysis method and by measuring changes in fluorescence. the extent of binding of fbp to the enzyme paralleled its activation. in addition, the binding constant for fbp increased in the presence of substrate, atp. these results suggest that fbp is ... | 1979 | 230181 |
| the influence of growth temperature on the heat stability of inorganic pyrophosphatase from crude extracts of bacillus stearothermophilus. | inorganic pyrophosphatase activity of crude extracts from bacillus stearothermophilus adapts its thermostability to the growth temperature of the cells. magnesium ions increase the stability of the enzyme in all cases. depending on the growth temperature of the cells two pyrophosphatase species differing in their molecular weights and heat stabilities have been detected. | 1979 | 232960 |
| a chemical approach to the fine structure of biomolecular complexes: the amino terminal region of the 50s ribosomal "a" protein from bacillus stearothermophilus. | an experimental approach and methodology are described for determining the reactive properties and ionization constants of individual functional groups of proteins within biomolecular complexes. the ionization constants and reactivities of the methionyl-l amino terminus and the lysyl-3 residue of the alanine rich 50s ribosomal "a" protein from bacillus stearothermophilus have been determined by an extension of the competitive labeling technique used by h. kaplan, k. j. stevenson, and b. s. hartl ... | 1975 | 234242 |
| production and purification of the thermophilic bacteriophage tp-84. | a new procedure for production and purification of the thermophilic bacteriophage tp-84 in high yields is described. cultures of bacillus stearothermophilus strain 10, enriched with nutrients to obtain heavy growth and to prevent sporulation and maintained at a ph of 6.5, were infected with the phage in a 100-liter fermentor. addition of magnesium chloride (0.01 m) and a temperature of 58-c were essential for maximal phage production. phage (5 times 1011 infective particles/ml) was precipitate ... | 1975 | 234714 |
| glyceraldehyde-3-phosphate dehydrogenase from bacillus stearothermophilus. | | 1975 | 236448 |
| glucose-6-phosphate isomerase from bacillus stearothermophilus. | | 1975 | 236463 |
| isolation and characterization of isocitrate lyase and malate synthase from bacillus stearothermophilus. | | 1975 | 236948 |
| pyruvate carboxylase from bacillus stearothermophilus: molecular size, biotin content and subunit constitution. | | 1975 | 236949 |
| mycotoxin bioassay, using bacillus stearothermophilus. | spores of bacillus stearothermophilus in standardized spore strips are pretreated with solutions of the mycotoxins aflatoxin b1, patulin, rubratoxin b, and diacetoxyscirpenol and subsequently incubated in a nutrient solution containing bromocresol purple as ph indicator. after 16.5 hr of inclubation the color of the indicator medium inoculated with untreated spore strips of b. stearothermophilus changes from purple to yellow; no color change occurs in the indicator medium inoculated with spore s ... | 1975 | 237876 |
| effect of ph on sporulation of bacillus stearothermophilus. | an improved broth medium was developed for high growth yields of bacillus subtilis var. niger ncib 8649, bacillus cereus ncib 9373, and bacillus stearothermophilus ncib 8919 and atcc 7953. sporulation was abundant (1.1 times 10-8 b. subtilis var. niger and 9.2 times 10-7 b. cereus per ml) at an initial ph of 7.0. sporulation of both strains of b. stearothermophilus took place (1.9 times 10-7 and 2.4 times 10-7/ml, respectively) in this medium when initial ph values of 7.7 to 8.7 were used. | 1975 | 238470 |
| synthesis of polyglycerol phosphate by bacillus stearothermophilus. | a particulate enzyme preparation from bacillus stearothermophilus synthesized 1,3-poly(glycerol phosphage) from cdpglycerol at an optimum ph of 8.0 and the reaction was stimulated by divalent cations. km for cdpglycerol was 0.18 mm. the synthesis was inhibited by cmp, cdp, and ctp and by concentrations of cdp-glycerol above 0.49 mm. the reaction was irreversible, the product had an average chain length of 8 glycerol units. about two thirds of the polymers were synthesized in entirety while the r ... | 1975 | 238960 |
| biochemical changes during sporulation of bacillus stearothermophilus. | the process of sporulation was studied in bacillus stearothermophilus. a medium is described that supports good growth and sporulation of the organism. in this medium, which contains glucose, salts, and amino acids, acetate starts to accumulate before any of the glucose is catabolized. enzymes of the tricarboxylic acid cycle are present at all times during growth and sporulation and are found in dormant spores. as the glucose in the culture is consumed, acetate rapidly increases and the ph of th ... | 1975 | 240496 |
| affinity chromatography on immobilised nucleotides. some applications to the purification of thermophilic dehydrogenases and kinases. | the effect of ph and temperature on the capacity and binding of bacillus stearothermophilus, alcohol dehydrogenase and phosphofructokinase to n6-(6-aminohexyl)-5'-amp-sepharose has been examined. specific elution from the substituted amp-sepharose was examined using a variety of cofactors, fragments of cofactors and substrates. a purification scheme for each enzyme on the substituted amp-sepharose using nucleotides and gradients of ph and salt is presented. interestingly, elevated temperature in ... | 1975 | 240692 |
| effect of storage conditions on the performance of bacillus stearothermophilus biological indicators. | | 1979 | 260940 |
| autoclaving of lubricated dental instruments. | test organisms forced mechanically into lubricated, rotating dental instruments (handpieces) were all killed during autoclaving at 134 degrees c for 8 min, even when protected by serum and oil. the test organisms were: escherichia coli, staphylococcus aureus, pseudomonas aeruginosa, candida albicans, and spores of bacillus stearothermophilus. also when testing the sterility of autoclaved simulated instrument surfaces (brass cylinders and pieces of a cotton fabric) which had been inoculated with ... | 1978 | 274800 |
| cluster characterization in iron-sulfur proteins by magnetic circular dichroism. | we report magnetic circular dichroism (mcd) spectra of 4-fe iron-sulfur clusters in the iron-sulfur proteins chromatium high-potential iron protein (hipip), bacillus stearothermophilus ferredoxin and clostridium pasteurianum ferredoxin. the mcd is found to vary significantly with cluster oxidation state but is relatively insensitive to the nature of the protein. the spectra obtained are compared with the corresponding spectra of iron-sulfur proteins containing 2-fe clusters. it is concluded tha ... | 1978 | 281679 |
| tetrahydrofolate-dependent biosynthesis of ribothymidine in transfer ribonucleic acids of gram-positive bacteria. | trimethoprim, an inhibitor that prevents tetrahydrofolate-dependent transmethylation reactions inbacteria, was used in a comparative study to discriminate between two possible biosynthetic pathways, either the s-adenosylmethionine or the tetrahydrofolate-dependent formation of ribothymidine (rt) in transfer ribonucleic acids (trna's) of several strains of gram-positive and gram-negative microorganisms. rt-deficient trna's accumulate in trimethoprim-treated gram-positive streptococcus faecium, st ... | 1977 | 318638 |
| host factor for coliphage q beta rna replication: presence in procaryotes and association with the 30s ribosomal subunit in escherichia coli. | the host factor required for in vitro coliphage q beta rna replication, a heat-stable rna binding protein present in uninfected escherichia coli, has been detected by both immunological and functional tests in acinetobacter calcoaceticus, klebsiella pneumoniae, pseudomonas aeruginosa and pseudomonas putida. it was not detectable by these criteria in bacillus stearothermophilus, bacillus subtilis, caulobacter crescentus, micrococcus lysodeikticus, rhodopseudomonas capsulata or saccharomyces cerev ... | 1977 | 329101 |
| sequence homologies between the tryptophanyl trna synthetases of bacillus stearothermophilus and escherichia coli. | | 1977 | 334569 |
| translational specificity of bacillus stearothermophilus ribosomes. | the translational specificity of bacillus stearothermophilus ribosomes was studied by determining the effectiveness of various synthetic rnas as templates at 37 degrees and at higher temperatures. the effectiveness of poly(g,u) was maximal at a g:u ratio of 1:3; it declined with lower g content because of reduced ribosomal affinity for the rna and, with higher g, because of interference by secondary structure. the effectiveness of poly(a,c,g,u) also declined when secondary structure was increase ... | 1978 | 343103 |
| binding oligonucleotides to escherichia coli and bacillus stearothermophilus 5 s rna. | | 1978 | 347090 |
| the nucleoside sequence of tyrosine trna from bacillus stearothermophilus. | the nucleotide sequence of trnatyr from b. stearothermophilus has been determined: pg-g-a-g-g-g-g-s4u-a-g-c-g-a-a-g-u-gm-g-c-u-a-a-m1a-c-g-c-g-g-c-g-g-a-c-u-q-u-a-ms2i6a-a-psi-c-c-g-c-u-c-c-c-u-u-u-g-g-g-u-u-c-g-g-c-g-g-t-psi-c-g-a-a-u-c-c-g-u-c-c-c-c-c-u-c-c-a-c-c-aoh. a combination of classical fingerprinting methods, partial nuclease p1 digestion and two-dimensional homochromatography and a rapid "read off" sequencing gel technique were used to establish the complete nucleotide sequence. | 1978 | 347395 |
| identification of escherichia coli and bacillus stearothermophilus ribosomal protein binding sites on escherichia coli 5s rna. | e coli [32p]-labelled 5s rna was complexed with e. coli and b. stearothermophilus 50s ribosomal proteins. limited t1 rnase digestion of each complex yielded three major fragments which were analysed for their sequences and rebinding of proteins. the primary binding sites for the e. coli binding proteins were determined to be sequences 18 to 57 for e-l5, 58 to 100 for e-l18 and 101 to 116 for e-l25. rebinding experiments of purified e. coli proteins to the 5s rna fragments led to the conclusion t ... | 1978 | 353489 |
| binding sites of e. coli and b. stearothermophilus ribosomal proteins on b stearothermophilus 5s rna. | the primary binding sites for bacillus stearothermophilus proteins b-l5 and b-l22 and the escherichia coli proteins e-l5, e-l18 and e-l25 on b. stearothermophilus 5s rna were determined by limited ribonuclease digestion of the corresponding 5s rna-protein complexes. the results obtained in this study are in agreement with our previous experiments in which the binding sites of e. coli and b. stearothermophilus proteins were determined for e. coli 5s rna and lead to the conclusion that the protein ... | 1978 | 353739 |
| aminoacyl-trna synthetases: affinity labeling of the atp binding site by 2', 3' -ribose oxidized atp. | homogeneous escherichia coli methionyl-, isoleucyl-, tryptophanyl-, and phenylalanyl-trna synthetases and bacillus stearothermophilus methionyl- and tyrosyl-trna synthetases are irreversibly inactivated by reaction of their active atp sites with the 2',3'-dialdehyde derivative of atp obtained by periodate oxidation. in each case, the amount of 14c-labeled dialdehyde derivative incorporated per molecule of inactivated enzyme appears consistent with the expected active stoichiometry of the synthet ... | 1978 | 353807 |
| an enhanced thermostability in thermophilic 5-s ribonucleic acids under physiological salt conditions. | the secondary structure of 5-s rrnas of thermus aquaticus (an extreme thermophile), bacillus stearothermophilus (a moderate thermophile) and escherichia coli (a mesophile) was compared using thermal denaturation techniques under varying ionic conditions. at a low ionic strength (10 mm k+), the tm of t. aquaticus 5-s rna differed by only 1 degrees c from that of e. coli rna and the molecule was fully denatured well below the optimum growth temperature of the thermophile. the internal na+, k+ and ... | 1978 | 363159 |
| potentiometric titrations and oxidation-reduction potentials of manganese and copper-zinc superoxide dismutases. | bovine erythrocyte superoxide dismutase and two manganese-containing superoxide dismutases have been reduced by the indirect coulometric titration method with methylviologen as the mediator-titrant. on the basis of the titration data the manganese-containing superoxide dismutases contain 1 g-atom of metal per mol of enzyme (dimer). e0' = +0.31 v for the enzyme from escherichia coli which exhibits a complicated ph dependence above neutral ph. the bacillus stearothermophilus manganese-containing e ... | 1979 | 380641 |
| adenosine triphosphate consumption by bacterial arginyl-transfer ribonucleic acid synthetases. | atp consumption by arginyl-trna synthetases from escherichia coli and bacillus stearothermophilus has been investigated by the firefly luciferin--luciferase assay. arginyl-trna synthetase from e. coli utilizes atp only for aminocylation of trna with a 1:1 stoicheiometry. in contrast, we have shown an adenosine triphosphatase activity of arginyl-trna synthetase from b. stearothermophilus in the absence of trnaarg. dowex chromatography revealed the formation of adp by the thermophile enzyme; under ... | 1979 | 384995 |
| comparative surfact structure of 16s ribosomal ribonucleic acid of 30s ribosomes of procaryotic cells. | ribonuclease t(1) treatment of 30s ribosomes of escherichia coli converts a large region at the 3' oh end of 16s ribosomal ribonucleic acid (rrna) to low-molecular-weight rna. the final 25 nucleotides at the 3' terminus of the molecule emerge relatively intact, whereas most of the region "upstream," for about 150 nucleotides, is converted to oligonucleotides. identical enzyme treatment generates a fragment of about 60 nucleotides from the middle of 16s rrna (section d'). to determine whether the ... | 1979 | 387717 |
| determination of base pairing in escherichia coli and bacillus stearothermophilus 5s rnas by infrared spectroscopy. | the extent of base pairing in escherichia coli and bacillus stearothermophilus 5s rnas was determined by infrared spectroscopy. from the infrared spectra taken at 20 degrees and 52 degrees c it is concluded that e. coli and b. stearothermophlius 5s rnas possess a large number of base pairs (table i). comparison of our results with those previously published using other methods leads to the conclusion that the structures of prokaryotic 5s rnas involve a large number of tertiary interactions, in w ... | 1979 | 388350 |
| kinetic evidence for an acyl-enzyme intermediate in d-alanine carboxypeptidases of bacillus subtilis and bacillus stearothermophilus. | the kinetics of hydrolysis and transpeptidation of the synthetic substrate diacetyl-l-lysyl-d-alanyl-d-alanine and of the natural substrate udp-acetylmuramyl pentapeptide and related compounds catalyzed by the d-alanine carboxypeptidases of bacillus subtilis and bacillus stearothermophilus in the presence of the nucleophiles hydroxylamine or glycine have been examined. these kinetic data suggest that an acyl-enzyme intermediate is formed in the first step of the reaction and that the transpeptid ... | 1977 | 404297 |
| gardimycin, a new antibiotic inhibiting peptidoglycan synthesis. | gardimycin, a new antibiotic, at 100 mug/ml, specifically inhibited cell wall synthesis and induced accumulation of uridine 5'-diphosphate-n-acetylmur-amylpentapeptide in whole cells of bacillus subtilis. the antibiotic was active in a particulate enzyme preparation from bacillus stearothermophilus: 60 mug/ml caused 50%, and 200mug/ml caused 100%, inhibition of peptidoglycan synthesis. suppression of peptidoglycan synthesis was accompanied by parallel accumulation of the lipid intermediate. this ... | 1977 | 404960 |
| microwave oven irradiation as a method for bacterial decontamination in a clinical microbiology laboratory. | exposure of 10 frequently isolated clinical pathogens to microwave irradiation resulted in total sterilization with 60 s. time exposure experiments done with commercially prepared test strips containing bacillus stearothermophilus spores indicated that 5-min exposure was adequate to insure sterility of small, contaminated loads. | 1977 | 410828 |
| inactivation of bacillus spores in dry systems at low and high temperatures. | a plot of the thermal resistance of bacillus subtilis var. niger spores (log d value) against temperature was linear between 37 and 190 degrees c (z = 23 degrees c), provided that the relative humidity of the spore environment was kept below a certain critical level. the corresponding plot for bacillus stearothermophilus spores was linear in the range 150 to 180 degrees c (z = 29 degrees c) but departed from linearity at lower temperatures (decreasing z value). however, the z value of 29 degrees ... | 1977 | 411885 |
| the binding site of ribosomal protein l1 from escherichia coli on the 23-s ribosomal rna from bacillus stearothermophilus. a possible base-pairing scheme differing from that proposed for escherichia coli. | the region of bacillus stearothermophilus strain nca 1503 23-s ribosomal rna protected from t1 ribonuclease digestion by the 50-s ribosomal subunit protein l1 from escherichia coli has been established. the sequence of 115 nucleotides is compared to the analogous region in e. coli. the similar behaviour of the rna towards the recognition of protein l1 may be explained in terms of secondary base-pairing, even though there exists almost 40% difference between the primary nucleotide sequences. | 1978 | 416958 |
| further evidence for the structure of the teichoic acids from bacillus stearothermophilus b65 and bacillus subtilis var. niger wm. | bacillus stearothermophilus b65 and bacillus subtilis var. niger wm both contain teichoic acids in their walls composed of glycerol, phosphate and glucose. the 13c nuclear magnetic resonance spectrum of b. stearothermophilus teichoic acid showed 13c-31p coupling on the signals from the c-5 and c-6 carbon atoms of the glucose molecule and an alpha-glucosidic linkage between glucose and the c-1 atom of the glycerol moiety. these data are consistent with a poly[glucosylglycerol phosphate] as the ce ... | 1978 | 417919 |
| purification, properties and specificity of the restriction endonuclease from bacillus stearothermophilus. | the restriction endonuclease bsti was purified from 70kg of bacillus stearothermophilus. the final product is at least 97% pure as judged by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis; this major protein species co-migrates with the enzyme activity on native polyacrylamide-gel electrophoresis and isoelectric focusing. pure restriction endonuclease bsti has a subunit mol.wt. of 26,000 and is probably a loosely associated dimer. the enzyme shows maximum activity at ph values betwee ... | 1979 | 426781 |
| improved procedure of purification of inorganic pyrophosphatase from bacillus stearothermophilus. | an improved method for isolation of inorganic pyrophosphatase (ec 3.6.1.1) from bacillus stearothermophilus is described. the enzyme was purified to more than 90% after two chromatographic steps. a molecular weight of 140,000 daltons was estimated by density gradient centrifugation. the isoelectric point was found to be 4.0. | 1979 | 429108 |
| identification of the amino acid residue modified in bacillus stearothermophilus alcohol dehydrogenase by the nad+ analogue 4-(3-bromoacetylpyridinio)butyldiphosphoadenosine. | 4-(3-bromoacetylpyridinio)butyldiphosphoadenosine was synthesized with a [carbonyl-14c]acetyl label. the reactive coenzyme analogue inactivates alcohol dehydrogenase from bacillus stearothermophilus by forming a covalent enzyme-coenzyme compound. the inactivation kinetics as well as the spectral properties of the modified enzyme after treatment with sodium hyposulphite suggest that the analogue is bound at the coenzyme binding site. b. stearothermophilus alcohol dehydrogenase modified with 14c-l ... | 1979 | 436831 |
| the effect of mg2+ on some properties of nucleotide-free elongation factor tu from bacillus stearothermophilus. | the nucleotide-free elongation factor from bacillus stearothermophilus provides a means to study the effect of mg2+ ions on various reactions of the protein. the binding of gdp to the protein is stimulated by mg2+. from comparative studies with other metal ions, particularly mn2+, it appears that this stimulation is due to the formation of a metal - gdp complex which is bound to the protein. protection against proteolysis by trypsin is afforded by both mg2+ and mg - gdp, but not by gdp alone. th ... | 1979 | 436834 |
| cleavage of a cooh-terminal hydrophobic region from d-alanine carboxypeptidase, a penicillin-sensitive bacterial membrane enzyme. characterization of active, water-soluble fragments. | d-alanine carboxypeptidase (cpase), a detergent-soluble penicillin-sensitive membrane enzyme of bacillus stearothermophilus, mr = 46,500, was digested with either trypsin or alpha-chymotrypsin to yield water-soluble fragments, designated t-cpase and chy-cpase, respectively, each of mr = approximately 45,000. these fragments were generated and purified in milligram quantities by digestion of cpase covalently immobilized on a penicillin affinity column. they retained full enzymatic activity, becam ... | 1979 | 438218 |
| the crystallization of protein bl17 from the 50 s ribosomal subunit of bacillus stearothermophilus. | | 1979 | 467654 |
| structural homologies in alanine-rich acidic ribosomal proteins from procaryotes and eucaryotes. | the amino acid composition and amino-terminal sequence have been determined for the alanine-rich, acidic ribosomal 'a' protein (equivalent to escherichia coli l7/l12) from three procaryotic cell types that live under extreme environmental conditions (arthrobacter glacialis, clostridium pasteurianum, and bacillus stearothermophilus) as well as from wheat germ, a eucaryote source. these data are compared with previously published 'a' protein sequences from other procaryotes and eucaryotes. all the ... | 1979 | 476516 |
| changes in heat resistance during storage of bacillus stearothermophilus spores from complex and chemically defined media. | | 1979 | 479057 |
| effects of divalent cations on thermophilic inorganic pyrophosphatase. | divalent cations were shown to affect the structure and thermostability of thermophilic inorganic pyrophosphatase [pyrophosphate phosphohydrolase ec 3.6.1.1] purified from bacillus stearothermophilus and thermophilic bacterium ps-3. the properties of the enzymes from the two sources were found to be very similar. the enzymes were very unstable to heart in the absence of divalent cations, being inactivated gradually even at 40 degrees c. however, they became stable to heat denaturation in the pre ... | 1979 | 479117 |
| the control of the synthesis of isocitrate lyase in a thermophilic bacillus. | from a strain of bacillus stearothermophilus, devoid of active pyruvate carboxylase, a mutant (ng-15) was selected that grew on acetate in the presence of glucose. this mutant differed from its parent organism in possessing high activities of isocitrate lyase when grown on all carbon sources tested except nutrient broth, in possessing unusually low activities of nadp+-dependent isocitrate dehydrogenase and in containing increased amounts of isocitrate. revertants of mutant ng-15 which regained t ... | 1979 | 479837 |
| comparison of the efficacy of steam sterilization indicators. | twenty-one commercially available chemical steam sterilization indicators were processed in an empty autoclave for various times at temperatures between 240 and 270 degrees f (ca. 116 and 132 degrees c). the time required to reach a sterilized reading at each temperature was plotted on a semilogarithmic time-temperature plot and compared with the time-temperature sterilization curve for bacillus stearothermophilus. five of the indicators had time-temperature kinetics similar to those of b. stear ... | 1979 | 485144 |
| cysteinyl-trna synthetase from bacillus stearothermophilus. a structural and functional monomer. | a procedure is described for the purification of cysteinyl-trna synthetase as a side product of a multi-enzyme isolation from bacillus stearothermophilus. the native and denatured enzyme are both shown to have a molecular weight of 54000 by gel filtration and sodium dodecyl sulphate/polyacrylamide gel electrophoresis respectively. fingerprinting and peptide counting indicate that the polypeptide chain has a nonrepeating primary structure. the enzyme has only one binding site for each of its subs ... | 1979 | 488099 |