proteolytic and esterolytic activity of a milk-clotting protease (mcp) from bacillus mesentericus strain 76. 197719296
isolation of proteolytic enzymes from bacillus mesentericus and a study of their properties. 197719687
relationship between spore formation and synthesis of extracellular protheases in bacillus mesentericus.two phases were established in the synthesis of extracellular proteases by bacillus mesentericus 73. when the growth rate was high, the synthesis of proteases was small. the maximum rate of the enzyme synthesis was observed when the growth decelerated and the production of spores started. the synthesis of alkaline protease and the formation of spores are susceptible to nitrogen-metabolite and catabolite repression. the ability to produce spores was not found in the s variant of bacillus mesenter ...197723485
[physicochemical properties of the proteinase preparations from bacillus subtilis and bacillus mesentericus]. 197831551
proceedings: mechanism of action of beta-d-xylosidase from bacillus pumilus. 197660963
determination of the binding parameters of beta-d-xylosidase from bacillus pumilus, using a fluorescent ligand [proceedings]. 197881002
mechanism of action of beta-d-xylosidase from bacillus pumilus prl b12: ph- and alpha-deuterium kinetic isotope effects [proceedings]. 197993945
bacillus pumilus polysaccharide cross-reactive with meningococcal group a polysaccharide.a polysaccharide, antigenically and structurally related to meningococcal group a polysaccharide, was isolated from bacillus pumilus sh-17. this enteric bacterium has been implicated as a source of natural meningococcal group a immunity (myerowitz et al., 1973). the b. pumilus polysaccharide was composed of a homopolymer of (1-6)-n-acetyl-manosamine-1-phosphate, glycerol phosphate teichoic acid-containing n-acetylglucosamine and alkali-labile alanine esters, and a mucopeptide. the cross-reaction ...1976184043
[microbial resistance to formaldehyde. i. comparative quantitative studies in some selected species of vegetative bacteria, bacterial spores, fungi, bacteriophages and viruses].the resistence of different microorganisms to formaldehyde was determined. as test objects served gram-negative and gram-positive vegetative germs (klebsiella pneumoniae, pseudomonas aeruginosa, salmonella paratyphi-b, staphylococcus aureus, streptococcus faecalis), bacterial spores (bacillus cereus, bacillus pumilus, bacillus stearothermophilus, bacillus subtilis), fungi (aspergillus niger, candida albicans), bacteriophages (escherichia coli phages, t1, t2, t3), and viruses (adenovirus, poliomy ...1976190825
sensitivity and resistance to glutaraldehyde of the hydrogen and calcium forms of bacillus pumilus spores. 1975237861
[effect of iron and manganese ions on gelatinase synthesis by bacillus mesentericus 316 m]. 1977325327
[effect of different sources of nutrition on the enzyme activity of bacillus mesentericus 316 m]. 1977325330
[optimizing the composition of the nutrient medium for accumulation of gelatinase by bacillus mesentericus 316 m]. 1977325334
[formation of organic acids by strains of bacillus mesentericus and bac. subtilis isolated from the rumens of cattle]. 1977405553
bactericidal activity of macromomycin, a antitumor antibiotic.susceptibility testing by the broth dilution method showed that all the gram-positive but only some of the gram-negative bacteria tested were susceptible to the antitumor antibiotic, macromomycin (mcr; nsc 170105). the minimal inhibitory concentration for the susceptible organisms was less than 3 mug/ml. the action of mcr was bactericidal; however, at very high concentrations (50 mug/ml and above) some organisms occasionally escaped death. none of the escaped organisms was resistant to mcr. in c ...1977406834
[antibiotic formation in plasmid and plasmid-free strains].antibiotic activity of three plasmid and three plasmid-free strains of bacillus pumilus was studied. the antibiotic activity was found in one plasmid and two plasmid-free strains. it was supposed that both the chromosomic and extrachromosomic genes could participate in regulation of the antibiotic production in bac. pumilus. it was shown that the antibiotics produced by bac. pumilus had a very narrow spectrum and inhibited multiplication only of several grampositive bacteria.1978417667
binding of 4-methylumbelliferyl-beta-d-ribopyranoside to beta-d-xylosidase from bacillus pumilus.the determination of the binding parameters of 4-methylumbelliferyl-beta-d-ribopyranoside, a fluorescent ligand of beta-d-xylosidase (exo-1,4-beta-xylosidase, ec from bacillus pumilus, is described. a single binding site per polypeptide chain (60 000 daltons) was found and the homogeneity of the binding sites in the dimeric or tetrameric forms of the enzyme were shown. the association constants, as a function of temperature and ionic strength, were obtained from equilibrium binding exp ...1978638199
study of the sulfhydryl groups of beta-d-xylosidase from bacillus pumilus. 1978639822
[effect of exogenous factors on extracellular alkaline ribonuclease synthesis in bacillus mesentericus].bacillus mesentericus was found to assimilate nucleic acids as a source of nitrogen and phosphorus. nucleic acids added to the medium as a source of nitrogen or phosphorus stimulated synthesis of ribonuclease. when washed bacterial cells were incubated for a short period of time in a fresh nutrient medium containing rna, synthesis of rnaase was also induced. synthesis of the enzyme was inhibited by high concentrations of chloramphenicol and actinomycin d, and stimulated by low concentrations of ...1978672681
[antibacterial activity and bacteriophages of bacillus pumilus].inducable defective phages analogous to those in bac. subtilis were found in 5 strains of bac. pumilus, 3 of which possessed antibacterial activity. a moderate bacteriophage was also induced in addition to the defective phage in strain atcc 12140 not producing the antibiotic. it was found that the antibacterial activity was not associated with the presence of phages in the cultures. the data on the electron microscopic investigations are presented.1978677836
d-values of bacillus pumilus spores on irradiated devices (inoculated product).the d-values of bacillus pumilus spores on various devices ranged from 0.14 to 0.23 mrads. the majority of devices displayed d-values equal to or less than the value obtained on filter paper. increased resistivity was also encountered.1978697370
beta-d-xylosidase from bacillus pumilus prl b12: hydrolysis of aryl beta-d-xylopyranosides.the influence of substituents on the binding and hydrolysis of several substituted beta-d-xylopyranosides by beta-d-xylosidase from bacillus pumilus prl b12 has been investigated. from a comparison of the inhibition constants of 1-thio-beta-d-xylopyranosides with the apparent michaelis-menten constants of the substrates, it followed that the latter constants are good approximations of the true equilibrium constants. the influence of the substituent on the rate and activation parameters is small. ...1978698980
sporulation-converting bacteriophages for bacillus pumilus.thirty-three sporulation-converting bacteriophages for bacillus pumilus nrs576 were assigned to two apparently unrelated groups on the basis of morphology and antiserum neutralization. bacterial sporulation mutants responded similarly (conversion or nonconversion) to representatives of both phage groups. evidence is presented indicating that pmb1 and related phages specify a restriction and/or modification system.1978702643
[effect of carbon sources on the yield and antifusariosis activity of an antibiotic preparation from bacillus mesentericus 1701]. 1978723663
stability of bacillus pumilus spore strips used for monitoring radiation sterilization.dried bacillus pumilus spore strips had a stable d-value of 0.17 to 0.18 mrad after 1 year at 5 or 25 c, but resistance was lost at 35 c. refrigeration did not have an adverse effect on resistance to irradiation. the presence of water at the time of initial preparation increased this resistance to 0.23 to 0.25 mrad. proper drying is essential to achieve reproducible results in sterility tests using usp-type biological indicators.1976779652
[microflora of active ooze participating in the decomposition of sulfanilic acid].microflora of domestic water can be a source of active ooze adapted to sulphanilic acid. adaptation of the microflora to sulphanilic acid at a concentration of 170-200 mg/l takes 6 to 8 days. the microflora of active ooze, immediately after adaptation, consists mainly of pseudomonas species, ps. denitrificans, ps. fluorescens, ps. striata, ps. putida, etc., and also of achromobacter stutzeri, achromobacter flavum, mycobacterium phlei, mycobacterium mucosum, bacillus mesentericus, bac. cereus, sa ...1975808686
plasmid deoxyribonucleic acid in bacillus subtilis and bacillus pumilus.two of eighteen strains of bacillus subtilis examined contained covalently closed circular duplex deoxyribonucleic acid (dna) of homogeneous size and buoyant density. strain atcc 15841 contained about 16 copies per chromosome of plasmid ppl1, a circular dna element having a molecular weight of about 4.7 times 10(6) and a buoyant density of 1.700. strain atcc 7003 contained about one to two copies per chromosome of plasmid ppl2. ppl2 had a molecular weight of about 46 times 10(6) and a buoyant de ...1975809424
[characteristics of spore-forming bacteria of the genus bacillus that break down caprolactam].five strains of sporeforming bacteria were isolated from sewage of capron industry and their morphological, cultural, as well as physiological and biochemical properties were investigated. four strains were identified as bacillus subtilis and one as bacillus pumilus. the cultures were able to grow in mineral medium with caprolactam as a source of carbon and nitrogen. the influence of growth conditions on the rate of caprolactam decomposition in synthetic medium was studied.1975809644
beta-d-xylosidase from bacillus pumilus. molecular properties and oligomeric structure.bacillus pumilus beta-d-xylosidase, purified by affinity chromatography, seems to be homogeneous, as judged by disc electrophoresis and gel filtration. the absorption coefficient at 280 nm, ao, 1% 1cm, determined by the dry weight method, is 1.78. the complete amino acid composition is determined. sedimentation velocity studies show the presence of two components with s20, w values of 10.0 s and 6.6 s. after glutaraldehyde cross-linking two, enzymically active, components, with apparent molecula ...1975810171
[effect of carbon and nitrogen sources and complex b vitamins on the synthesis of alkaline protease by different strains of bacillus mesentericus and bacillus subtilis].the effect of different sources of carbon, nitrogen, amino acids and vitamins on the synthesis of alkaline proteases by the stock and mutant strains of bacillus mesentericus and by the natural strain of bacillus subtilis-12 has been investigated. the maximum synthesis of alkaline protease has been obtained in the media containing starch or its hydrolysates--dextrine and maltose as the carbon source. ammonium phosphate and casein as the nitrogen source prove to be optimal for bac. mesentericus an ...1975813199
[lethal and mutagenic effect of n-methyl-n'-nitro-n-nitrosoguanidine on bacillus mesentericus]. 1975814385
[study of the action of low-intensity uhf electromagnetic fields on bacillus mesentericus and pseudomonas fluorescens]. 1976821547
bacillus pumilus plasmid ppl10: properties and insertion into bacillus subtilis 168 by transformation.bacillus pumilus atcc 12140 harbors 10 or more copies per chromosome of each two small plasmids. variants of this strain were isolated which were sensitive to a killing activity produced by the plasmid-containing parent. each of 24 such sensitive (s) variants tested lacked detectable levels of supercoiled deoxyribonucleic acid. transduction of s variants to the kill+ phenotype was performed using phage pbp1 propagated on a mutant of atcc 12140, designated strain l10, that remained kill+ but reta ...1976821919
low-frequency, pbsi-mediated plasmid transduction in bacillus pumilus.three bacteriophages were tested for ability to transduce the plasmid of ppl10 between w mutant derivatives of bacillus pumilus nrs 576. phage pbp1- and pmb1-generated plasmid transductants occurred at about 10% the frequency of transductants for a chromosome marker. phage pbs1-generated plasmid transductants occurred at less than 0.1% the frequency of transductants for a chromosome marker. possible reasons for the extremely reduced capacity of pbs1 to generate plasmid transductants are discusse ...1976821920
[peptidases of preparations of proteolytic enzymes from several species of bacteria, molds and actinomycetes].the activity of carboxy- and aminopeptidases of enzymic preparations obtained from bacteria bacillus subtilis 1 m, 17, bacillus mesentericus 100/26, 100/56, 16; pseudomonas aeruginosa 1/7a, 1/4; bacillus thermophilicus s. sp. nov b-22, moulds aspergillus oryzae 1-746, aspergillus flavus 716 and actinomycete streptomyces griseus 32-13 was studied. in the bacterial preparations the proteolytic activity was 1.5-2.5 times lower than in the fungal and actinomycete preparations. the carboxypeptidase a ...1976825850
alterations in radiation resistance of bacillus pumilus e601 spores. 1977841009
bacteriophage conversion of spore-negative mutants to spore-positive in bacillus pumilus.a pseudolysogenic phage, pmb1, was isolated from soil on the basis of its ability to increase the sporulation frequency of the oligosporogenic bacillus pumilus strain nrs 576 (sporulation frequency, less than 1%). several spore-negative mutants (sporulation frequency, less than 10-8) derived from strain nrs 576, which were converted to spore positive by infection with pmb1, were subsequently identified. pmb1 repeatedly grown on a given spore-negative mutant (e.g., gw2) converted gw2 cells to spo ...1977857056
selective plasmid transduction in bacillus pumilus.the inducible temperate bacteriophage phi75 and a clear-plaque-forming variant, phi75c1, mediated transduction of a 4.4 x 10(6)-dalton multicopy bacillus pumilus plasmid, ppl10, at frequencies of 10(-5) to 10(-6) transductants per plaque-forming unit. phi75- and phi75c1-mediated transduction of several chromosome markers tested did not occur at a detectable frequency. phi75-mediated plasmid transducing activity resides in particles that are similar to infectious particles in sedimentation veloci ...1977893340
[separation and purification of an enzymatic preparation from bacillus mesentericus]. 1977904489
host function specified by bacillus pumilus plasmid ppl7065.plasmid ppl7065 ( approximately 4.7 x 10(6) daltons; approximately 20 copies per chromosome) determines the production of, and immunity or resistance to, a killing activity in strains of bacillus pumilus. plasmid ppl7065 is compatible with plasmid ppl576 ( approximately 28 x 10(6) daltons; approximately 2 copies per chromosome).1977907334
hydrolysis of para-substituted aryl beta-d-xylopyranosides and beta(1 leads to 4)xylo-oligosaccharides by an induced beta-d-xylosidase from bacillus pumilus prl b12 [proceedings]. 1977913794
[amino acid regulation of l-asparaginase formation in bacillus mesentericus].the role of amino acids in regulation of l-asparaginase formation was studied in bacillus mesentericus 43a. asparagic acid and, to a less extent, asparagine repress biosynthesis of the enzyme. glutamic acid, glutamine, and other 15 studied amino acids, added separately at a concentration of 10 or 20 mm to the growing culture, have no effect on the activity of the enzyme. addition of a combination of all 18 amino acids, each at a concentration of 4 mm, to the culture represses the activity by 64% ...1976933871
the site-specific deoxyribonuclease from bacillus pumilus (endonuclease r.bpu1387).a new site-specific endonuclease (dnase) was isolated from the cells of bacillus pumilus ahu 1387 strain. this enzyme (endonuclease r.bpu 1387) introduced double-stranded scissions at unique sites on dna's of coli phage lambda, lambdadvl, coli phage t7, bacillus phage phi105c, bacillus phage sp10, and simian virus 40, in the presence of magnesium ion. the activity was stimulated by the presence of nacl.19761018024
[extracellular nuclease of bacillus mesentericus].bacillus mesentericus is found to secrete three type of nucleases: alkaline ribonuclease (ec, acidic ribonuclease (ec and ca2+-activated exonucleease (ec these nucleases are purified and characterized. they are similar to those from bac. subtilis in main biochemical and physico-chemical properties and in their chromatographical behaviour. studying physiological functions of bac. mesentericus extracellular nucleases, it is shown that bacteria, which are capable to pr ...19761022289
[regulation of l-asparaginase biosynthesis in mutants of bacillus mesentericus 43a, poorly growing in the presence of aspartic acid].biosynthesis of l-asparaginase (ec was inhibited in the growing culture of bac. mesentericus 43a on addition of l-aspartic acid (20 mm). my treatment with methyl nitrosourea (2 mg/ml) mutants were obtained, which grew poorly on aspartic acid used as the only source of carbon and nitrogen. the aspartic acid did not repress the asparaginase biosynthesis in 8 strains, found between the mutants. in six of these mutants the asparaginase biosynthesis was inhibited by means of the type of cata ...19761025895
identification and quantitation of capsular antigen in capsulated and noncapsulated strains of haemophilus influenzae type b by crossed-immunoelectrophoresis.sonicated preparations of capsulated haemophilus influenzae type b, two of its spontaneous mutants, one containing patches of capsules (class i variant) and the other noncapsulated (class ii variant), and a noncapsulated strain of h. influenzae type d were analyzed by crossed-immunoelectrophoresis using unadsorbed antiserum to capsulated h. influenzae type b. twenty common antigens were present in all four cultures. two type b-specific antigens were also identified in the three h. influenzae typ ...19761085750
heteroimmunization to the capsular polysaccharide of haemophilus influenzae type b induced by enteric cross-reacting bacteria.cross-reacting escherichia coli strains easter and 89 and bacillus pumilis fed to newborn rabbits and e. coli fed to adult rhesus monkeys did not exert untoward reactions. the e. coli regularly colonized the newborns' intestinal tract from 1 to 7 weeks. high doses of e. coli were necessary to colonize adult primates. colonization occurred in fewer newborn rabbits and lasted only 1 to 3 weeks with b. pumilis. colonized newborn rabbits and adult rhesus had an active haemophilus influenzae type b ( ...19751091559
characterization of bacillus pumilus e601 spores after single sublethal gamma irradiation treatments.eighteen survivor strains of bacillus pumilus e601 have been isolated after single sublethal irradiation treatments with 60co. primary isolation was based on the loss of motility and pellicle formation. however, with subsequent subcultivation, eight isolates reverted back to the standard of exhibiting motility and pellicle formation. characteristics of the isolates include alterations in space radiation resistance and in the amino acid requirements for spore germination and outgrowth. other alte ...19751110491
evidence for a nonrandom base sequence in a bacillus pumilus plasmid: ecor1 endonuclease digestion of ppl576.ecor1 endonuclease digested the bacillus pumilus plasmid ppl576 (molecular weight similar to 28 x 10-6) into three distinct size classes of linear fragments. the molecular weights of the fragments are 13.0 x 10-6, 0.5 x 10-6, and 6.5 x 10-6 by sucrose gradient analysis. by electron microscope analysis the three fragments account for about 99% of the intact plasmid. these results indicate that ppl576 molecules contain a nonrandom base sequence, and are consistent with the interpretation that ppl5 ...19751141201
properties of the ribosomes of antibiotic producers: effects thiostrepton and micrococcin on the organisms which produce them.ribosomes of streptomyces azureus, which produces thiostrepton, are resistant to thiostrepton by virtue of being unable to bind the antibiotic. these ribosomes are also resistant to a number of other antibiotics (the thiostrepton group) which may share a common ribosomal binding site and a common mode of action with thiostrepton. conversely, bacillus pumilis and a strain of micrococcus, which produce the (probably identical) antibiotics micrococcin p and micrococcin, respectively, possess riboso ...19751155929
efficacy of the inactivation of bacterial spores in white petrolatum and a hydrophilic ointment by gamma evaluate the possibilities of using gamma irradiation for the sterilization of ointments, the effect of irradiation on spores of bacillus pumilus and bacillus sphaericus in dry material and in two different kinds of ointments was studied. the results indicate that for sterilization purposes irradiation was less effective in white petrolatum as compared to irradiation in the dry state. no such protective effect was found in a hydrophilic ointment. accordingly, the sterilization dose needed for ...19751190757
[study of catalase and proteolytic activities of different variants of bacillus mesentericus].catalase and proteolytic activity of the culures and morphological variants of bacillus mesentericus fuscus, bac. mesentericus vulgatus were studied. the variants were obtained as a result of prolonged cultivation of the stock strains in the potato mash under the layer of vaseline oil. the level of catalase activity varies in different morphological variants of the same culture, changes with age and depends on the storage conditions. the catalase activity in the rough, smooth and papillar varian ...19751208411
purification of milk-clotting protease from bacillus mesentericus strain 76.a two-step procedure for the isolation of pure enzyme from bacillus mesentericus strain 76 is developed. ion-exchange chromatography on cm-sephadex-c50 is used as a second step after precipitation from ethanol. the pure enzyme preparation, obtained after gel filtration on sephadex-g25 of the ion-exchange chromatographically separated product, possess the highest specific activity achieved. the homogeneity of the final preparation is proved by determination of the n-terminal amino acid (arg) and ...1978681078
recombination between compatible plasmids containing homologous segments requires the bacillus subtilis rece gene product.plasmid psl103 was previously constructed by cloning a trp fragment (approximately 2.3 x 10(6) daltons) from restriction endonuclease ecori-digested chromosome dna of bacillus pumilus using the neomycin-resistance plasmid pub110 (approximately 2.8 x 10(6) daltons) as vector and b. subtilis as transformation recipient. in the present study the ecori trp fragment from psl103 was transferred in vitro to ecori fragments of the bacillus plasmid ppl576 to determine the ability of the plasmid fragments ...197896091
preparation, separation and degradation of oligouronides produced by the polygalacturonic acid transeliminase of bacillus pumilus.production of the end products of polygalacturonic acid degradation on a large scale was done by reacting free galacturonic acid with bacillus pumilus polygalacturonic acid transeliminase (pate, ec to obtain a mixture of the barium salts of several oligouronides. small amounts of the unsaturated oligouronides were separated by paper chromatography. large quantities of unsaturated oligouronides were separated on a ag-1-x8 (formate) column by applying a sample of mixed oligouronides and s ...19761245579
[formation of bactericidal substances by cosmarium impressulum (author's transl)].1. an already published test method for detecting bactericidal substances in paper chromatograms was further improved. 2. in cultures of cosmarium impressulum free from bacteria, two bactericidal substances were found in the ether extracts from the algae and two others in the extracts of the culture medium. the are active against some or all bacteria testes (serratia marcescens, pseudomonas fluorescens; aerobacter aerogenes or bacillus pumilus). 3. if the culture medium of cosmarium or another d ...19761275650
the effect of ribosomal protein s1 from escherichia coli and micrococcus luteus on protein synthesis in vitro by e. coli and bacillus subtilis.we have designed a set of nine plasmids containing the bacillus pumilis cat gene with one of three shine-dalgarno (sd) sequences (weak, strong or stronger) and one of three initiation codons (aug, gug or uug). these constructions have been used to determine the effect of ribosomal protein s1, sd and initiation codon sequences and escherichia coli ribosomal protein s1 on translation in vitro by e. coli and b. subtilis ribosomes. translation of these nine constructions was determined with three ty ...19921283001
[extracellular ribonuclease from bacillus pumilus].the extracellular ribonuclease (rnase bp) was isolated from the cultural medium filtrate of bacillus pumilus by ammonium sulfate precipitation and two stages of ion-exchange chromatography on carboxymethyl- and phospho-cellulose columns. the amino acid composition and n-terminal amino acid residue have been determined. the kinetic parameters of cleavage reaction of synthetic polynucleotides have been measured. according to their structural homology rnase bp has been shown to be similar to rnases ...19921302501
[transposition of tn917 in bacillus pumilus].transposition tn917 was introduced into bacillus pumilus 289 by protoplast transformation with plasmid ptv32. the temperature-sensitive replication property of ptv32 was maintained in b. pumilus. tn917 was transposed efficiently in b. pumilus with 4.8 x 10(-4) transposition rate. the yield of auxotrophs was about 0.65% in all insertional mutants. it indicated a prospects for the use of tn917 as a tool for insertional mutagenesis and genetic manipulation in b. pumilus.19921329360
site-directed mutagenesis at aspartate and glutamate residues of xylanase from bacillus elucidate the reaction mechanism of xylanase, the identification of amino acids essential for its catalysis is of importance. studies have indicated the possibility that the reaction mechanism of xylanase is similar to that of hen's egg lysozyme, which involves acidic amino acid residues. on the basis of this assumption, together with the three-dimensional structure of bacillus pumilus xylanase and its amino acid sequence similarity to other xylanases of different origins, three acidic amino ...19921359880
purification and some properties of dipeptidyl carboxypeptidase from bacillus intracellular protease from a bacterium, bacillus pumilus hl721, was purified about 5000-fold by chromatography with a q-sepharose fast flow column, tsk-gel ha-1000 glass column, and tsk-gel g3000swxl column using bz-gly-ala-pro as a substrate. the enzyme was the most active at ph around 7.5 and stable from 4.5 and 8.0. the enzyme activity was inhibited by cu2+, edta, n-ethylmaleimide, o-phenanthroline, and p-chloromercuribenzoic acid. the molecular weight of the enzyme was 155,000 by gel fil ...19901368555
enzymatic properties of dipeptidyl carboxypeptidase from bacillus pumilus.enzymatic properties of dipeptidyl carboxypeptidase (dcp) from bacillus pumilus were investigated. the enzyme was more active on tri- and tetrapeptides than angiotensin-converting enzyme (ace) from rabbit lung. the presence of chloride ion is essential for the hydrolysis. the km value of angiotensin i for the enzyme was 0.119 x 10(-3) m. the enzyme was not inhibited by the mammalian ace inhibitors lisinopril and enalaprilat. the enzyme is readily inhibited by edta but restored by co2+, mn2+, and ...19911368711
bpuai, a novel bbsi and bbvii isoschizomer from bacillus pumilus recognizing 5'-gaagac-3'. 19921408773
purification, properties, and partial amino acid sequences of thermostable xylanases from streptomyces thermoviolaceus opc-520.two types of xylanases (1,4-beta-d-xylan xylanohydrolase, ec were isolated from the culture filtrate of a thermophilic actinomycete, streptomyces thermoviolaceus opc-520. the enzymes (stx-i and stx-ii) were purified by chromatography with deae-toyopearl 650 m, cm-toyopearl 650 m, sephadex g-75, phenyl-toyopearl 650 m, and mono q hr. the purified enzymes showed single bands on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the molecular weights of stx-i and stx-ii were 54,000 ...19921539982
systemic bacillus species infection mimicking listeriosis of pregnancy.bacillus species are increasingly recognized as agents of infection in humans. these organisms are ubiquitous in nature and can cause clinical illness ranging from transient bacteremia to serious systemic infection. we describe a pregnant intravenous drug abuser with fever, constitutional symptoms, and premature labor. her blood cultures yielded gram-positive bacilli, and her clinical course was consistent with systemic listeriosis of pregnancy. pathological examination of the placenta revealed ...19921562662
a bifunctional xylanase encoded by the xyna gene of the rumen cellulolytic bacterium ruminococcus flavefaciens 17 comprises two dissimilar domains linked by an asparagine/glutamine-rich sequence.the nucleotide sequence of the xyna gene of ruminococcus flavefaciens 17 was determined and found to consist of a 2862bp open reading frame beginning with a ttg start codon. the predicted product, xyla, consisted of distinct amino-terminal (a) and carboxy terminal (c) domains (248 amino acids, including a putative signal sequence, and 332 amino acids, respectively) linked by a repetitive sequence (b, 374 amino acids) extraordinarily rich in asparagine (45%) and glutamine (26%) residues. domains ...19921584021
insertion of tn916 into bacillus pumilus plasmid pmgd302 and evidence for plasmid transfer by part of an effort to develop systems for genetic analysis of strains of bacillus pumilus which are being used as a microbial hay preservative, we introduced the conjugative enterococcus faecalis transposon tn916 into b. pumilus atcc 1 and two naturally occurring hay isolates of b. pumilus. b. pumilus transconjugants resistant to tetracycline were detected at a frequency of approximately 6.5 x 10(-7) per recipient after filter mating with e. faecalis cg110. southern hybridization confirmed the ...19911658832
[various physico-chemical properties of the amylolytic complex from bacillus mesentericus].some physico-chemical properties of the bacillus mesentericus amylolytic complex were studied, and optimal conditions of starch hydrolysis (ph 7.5-8.0; 45 degrees c) were found. the half-life of amylases at 50 degrees was 75 min. the heat stability of the enzymes increased in the presence of ca2+ ions. amylase was stable at ph 7-9 and readily inactivated at ph below 6.0. by physical and chemical characteristics the complex was close to analogous preparations from bacillus alkalophilic strains. i ...19901693427
sequence and properties of beta-xylosidase from bacillus pumilus ipo. contradiction of the previous nucleotide sequence.the nucleotide sequence of the beta-xylosidase (xynb) gene from bacillus pumilus has been reported previously [moriyama, h., fukusaki, e., crespo, j.c., shinmyo, a. & okada, h. (1987) eur. j. biochem. 166, 539-545]. however, the sequence identified in the present study is quite different from the previously reported one. the total length of the psti--ecori fragment of a plasmid poxn295 containing the xynb gene is 2201 bp from our sequencing, while the length of the fragment in the previous data ...19911765080
[influence of carbenicillin on pharmacokinetics of ribostamycin in 7 volunteers when they were used combinedly].the experiment consists of two groups, namely the ribostamycin (rib) group and the rib and carbenicillin (car) combination group. there were 7 healthy volunteers in each group. the rib concentration in blood and urine was detected with the improved microbiology method. the organism tested was bacillus pumilus. the results demonstrated that the drug serum level was in line with the one-compartment open model. the parameters such as k, ka, t1/2, t1/2a, tp, cmax, and auc in the rib group and the co ...19911781284
complex between the subtilisin from a mesophilic bacterium and the leech inhibitor eglin-c.the alkaline proteinase from the mesophilic bacterium bacillus mesentericus has been crystallized in a 1:1 complex with the inhibitor eglin-c from the medical leech. the crystals have cell dimensions of a = 43.0, b = 71.9, c = 48.3 a and beta = 110.0 degrees and are in the space group p2(1). three-dimensional data to 2.0 a have been recorded on film from a single crystal. the orientation and position of the complex in the unit cell have been established using the refined coordinates of subtilisi ...19911793542
isolation and characterization of chromosomal promoters of streptococcus salivarius subsp. thermophilus.a promoter probe vector, ptg244, was constructed with the aim of isolating transcription initiation signals from streptococcus thermophilus (streptococcus salivarius subsp. thermophilus). ptg244 is based on the escherichia coli-streptococcus shuttle vector ptg222, into which the promoterless chloramphenicol acetyltransferase gene of bacillus pumilus (cat-86) was cloned. random sau3a fragments from the s. thermophilus a054 chromosomal dna were cloned upstream of the cat-86 gene by using e. coli a ...19911854195
modification of a zinc proteinase from bacillus mesentericus strain 76 by diethylpyrocarbonate.diethylpyrocarbonate (depc) inactivated the neutral zinc proteinase from bacillus mesentericus strain 76/bacillus subtilis (mcp 76) by ethoxycarbonylation completely. exposure of the enzyme to depc together with the competitive inhibitor z-l-phenylalanine prevented the loss of activity toward both peptide and protein substrates. treatment with hydroxylamine restored the catalytic properties of the modified mcp 76 to that of the native enzyme. after chymotryptic digestion of ethoxycarbonylated mc ...19911894447
an efficient cyanide-degrading bacillus pumilus strain.a gram-positive, aerobic, endospore-forming bacterium was isolated by an enrichment technique for the ability to degrade cyanide and was identified as a bacillus pumilus strain. the bacterium rapidly degraded 100 mg l-1 of free cyanide in the absence of added inorganic and organic substances. the ability to degrade cyanide was linked to the growth phase and was not exhibited before late exponential/early stationary phase. cyanide-degrading activity could not be induced before this time by the ad ...19911919513
nucleotide sequence and phylogeny of a chloramphenicol acetyltransferase encoded by the plasmid pscs7 from staphylococcus aureus.the nucleotide sequence of the chloramphenicol acetyltransferase gene (cat) and its regulatory region, encoded by the plasmid pscs7 from staphylococcus aureus, was determined. the structural cat gene encoded a protein of 209 amino acids, which represented one monomer of the enzyme chloramphenicol acetyltransferase (cat). comparisons between the amino acid sequences of the pscs7-encoded cat from s. aureus and the previously sequenced cat variants from s. aureus, staphylococcus intermedius, staphy ...19911929326
toxin production by bacillus pumilus.two strains of bacillus pumilus (m11 and m38) and one strain each of bacillus cereus (m27), bacillus subtilis (m67), and enterobacter agglomerans (m14) were identified from the air of lancashire cotton mills. these strains were tested for cytopathic effects in vero cells; b pumilus and b cereus strains were also examined for haemolytic activity, lecithinase production, and proteolytic action on casein. rounding and clumping of the vero cells occurred after the addition of supernatants prepared f ...19912066422
the structure of the trpe, trpd and 5' trpc genes of bacillus pumilus.the nucleotide (nt) sequences of the bacillus pumilus trpe, trpd and 5' portions of trpc genes have been determined. genetic analysis suggested the presence of an internal promoter upstream from the trpc gene, yet no typical consensus sequences were found. the nt and amino acid sequence homologies between the b. pumilus, bacillus subtilis and escherichia coli trp genes are presented.19902110100
antimicrobial properties of n3-(iodoacetyl)-l-2,3-diaminopropanoic acid-peptide conjugates.six peptide conjugates consisting of either norvaline, methionine, or lysine and n3-(iodoacetyl)-l-2,3-diaminopropanoic acid--a strong, irreversible inactivator of bacterial and fungal glucosamine-6-phosphate synthase--were synthesized and their antibacterial and antifungal activities were evaluated. antimicrobial potencies of these peptides were correlated with their transport and cleavage rates inside the cells. bacteriolysis of bacillus pumilus cells and inhibition of [14c]glucose incorporati ...19902120441
[joint culturing of streptococcus lactis, strain mgu with proteus vulgaris and bacillus mesentericus].nisin synthesis by streptococcus lactis, strain mgu, grown as a combined culture together with proteus vulgaris and bacillus mesentericus under stationary conditions or with stirring does not depend on the quantity of inoculated associated cells. nisin synthesis in the combined culture drops down by 10-20% at the initial ph 7.5 of the growth medium which is unfavourable for s. lactis producing nisin. the level of nisin biosynthesis does not rise when the ph of the medium is adjusted (either natu ...19902124650
pumilacidin, a complex of new antiviral antibiotics. production, isolation, chemical properties, structure and biological antibiotic pumilacidins a, b, c, d, e, f and g were isolated from the culture broth of a strain of bacillus pumilus. they are cyclic acylheptapeptide composed of a beta-hydroxy fatty acid, two l-leucine, two d-leucine, l-glutamic acid, l-aspartic acid and l-isoleucine (or l-valine). pumilacidin components were inhibitory to herpes simplex virus type 1 and h+, k(+)-atpase and demonstrated antiulcer activity in rat.19902157695
association of bacteria with the fungal fermentation of soybean tempe.bacteria grew to viable populations of 10(8)-10(9) cfu/g during the fermentation of soybeans into tempe with the fungus, rhizopus oligosporus. bacillus pumilus and b. brevis were the predominant bacterial species, reaching populations of approximately 10(8) cfu/g during the 48 h fermentation. species of streptococcus faecium, lactobacillus casei, klebsiella pneumoniae and enterobacter cloacae also contributed to the fermentation and achieved populations of 10(6)-10(7) cfu/g.19902179196
the structure of the trpe, trpd and 5' trpc genes of bacillus pumilus. 19902227447
[characteristics of hydrolase biosynthesis in bacillus mesentericus grown on various media].the dynamics of the consumption of major carbon and nitrogen sources and the biosynthesis of hydrolytic enzymes were studied in bacillus mesentericus grown on semisynthetic media. conditions were chosen that provide the obtaining of the culture liquid with predominantly proteolytic or amylolytic activity. the replacement of maltose with native starch resulted in more intensive accumulation of the biomass and hydrolytic enzymes, and in more rapid (by 3-5 hr) transformation from the logarithmic to ...19902235925
[disinfection of ophthalmologic tonometers].the authors describe an automatic instrument of their own design for disinfection of ophthalmological tonometers by germicide radiation. the tests of effectiveness against microbes (staphylococcus aureus, escherichia coli, pseudomonas aeruginosa), spores of bacillus mesentericus, adenoviruses and viruses of herpes simplex provided evidence of a high disinfectant potency of the apparatus.19902249269
the comparative efficacy and safety of 5% povidone-iodine cream for topical antisepsis.a 5% povidone-iodine cream (betadine cream, the purdue frederick company, norwalk, conn.) was tested extensively to determine its safety and efficacy. results of in vitro microbiologic comparison found that a representative panel of vegetative test organisms couldn't be recovered after 60 seconds or less exposure to povidone-iodine (pvp-i) cream, whereas the kill time of the combination-antibiotic cream (neosporin cream, burroughs wellcome, research triangle park, nc) exceeded 15 minutes for at ...19902271079
primary structure of a zinc protease from bacillus mesentericus strain 76.the amino acid sequence of the neutral zinc protease from bacillus mesentericus strain 76 (mcp 76) has been determined by using peptides derived from digests with trypsin, chymotrypsin, and cyanogen bromide and from cleavage with o-iodosobenzoic acid. the peptides were purified by means of gel filtration and reversed-phase high-performance liquid chromatography and analyzed by automatic sequencing. the protein contains 300 amino acid residues. it proved to be identical with the neutral protease ...19902302386
model for inactivation and disposal of infectious human immunodeficiency virus and radioactive waste in a bl3 facility.a method is described for autoclaving low levels of solid infectious, radioactive waste. the method permits steam penetration to inactivate biologic waste, while any volatile radioactive compounds generated during the autoclave process are absorbed. inactivation of radiolabeled infectious waste has been problematic because the usual sterilization techniques result in unacceptable radiation handling practices. if autoclaved under the usual conditions, there exists a high probability of volatiliza ...19902310182
[hydrolase biosynthesis by morphological variants of bacillus mesentericus as dependent on the storage conditions].a method used to prepare the inoculum (a strain of bacillus mesentericus, a producer of a complex of hydrolytic enzymes) has been studied for its effect on the activity of proteinases and amylases under submerged cultivation in fermenters. optimal conditions for the culture storage and inoculum cultivation are developed to obtain standard enzymic preparations.19902352501
sigma 29-like protein is a common sporulation-specific element in bacteria of the genus bacillus.a monoclonal antibody specific for an antigenic determinant on the bacillus subtilis sporulation-induced sigma factor sigma 29 reacted with proteins similar in size to sigma 29 in extracts of sporulating bacillus licheniformis, bacillus amyloliquifaciens, bacillus cereus, bacillus natto, and bacillus pumilus but not in extracts prepared from vegetatively growing cultures of these bacteria. these results indicate that rna polymerase modifications, initially described for b. subtilis, are likely t ...19852415506
structure and expression of genes coding for xylan-degrading enzymes of bacillus pumilus.the complete nucleotide sequence of the beta-xylosidase gene (xynb) of bacillus pumilus ipo and its flanking regions was established. a 1617-bp open reading frame for beta-xylosidase, a homodimer enzyme, was observed. the amino acid sequence of the n-terminal region and the molecular mass 62607 da) of the beta-xylosidase subunit, deduced from the dna sequence, agreed with the result obtained with the purified enzyme. the shine-dalgarno sequence was found 8 bp upstream of the initiation codon, at ...19872440680
[substrate specificity of enzymes from bacillus mesentericus].the proteolytic enzymes of the sporogenous bacillus mesentericus strains 64 and 8 were tested for their ability to hydrolyse different protein substrates. the enzymes were isolated using affinity chromatography on bacillichine-silochrome, and eluted with 25% isopropanol in 0.05 m tris-hcl buffer, ph 8.0-8.4, containing 0.01 m cacl2. casein, hemoglobin, elastin, albumin and synthetic peptides, z-l-ala-ala-leu-pna and z-l-ala-gly-leu-pna, were used as substrates. the activity of esterase was assay ...19892482930
flow microcalorimetric assay of antibiotics--ii. neomycin sulphate and its combinations with polymyxin b sulphate and zinc bacitracin on interaction with bacillus pumilus (nctc 8241).a flow microcalorimetric assay for neomycin has been developed which is monitored through interaction of the antibiotic with bacillus pumilus as the test organism. the assay has better reproducibility (relative standard deviation 2.3%) and is more sensitive than conventional microbiological bioassay (0.5-2 micrograms ml-1). the effects of combinations with zinc bacitracin, with polymyxin b sulphate, and with both zinc bacitracin and polymyxin b sulphate (both in equimolar proportions), and in th ...19892490094
indolinone derivatives as potential antimicrobial agents.1-substituted aminomethyl-3-cyclohexylthiosemicarbazone-2-indolinones (i) were tested for their antibacterial activity against bacillus pumilis, bacillus brevis and bacillus megaterium and antifungal activity against aspergillus flavus, aspergillus fumigatus and aspergillus niger. the majority of the compounds were found to exhibit promising antibacterial and antifungal activities. these compounds were also screened for their antiviral action against tobacco mosaic virus in nicotiana glutinosa p ...19892501946
membrane-bound succinate dehydrogenase of bacillus pumilus strain 5: effects of modulators of monoelectron transfer.the membrane-bound succinate dehydrogenase (sdh; ec of bacillus pumilus strain 5 was investigated as succinate:ferricyanide oxidoreductase activity at 27 degrees c. a km of 8.3 x 10(-3) m was obtained, and the vmax was 1.8 x 10(-6) mole succinate dehydrogenated min-1 mg-1 membrane protein, at a substrate (succinate) concentration below 40 x 10(-3) m. above this succinate concentration the km was 102 x 10(-3) m and the vmax was 3.7 x 10(-6) mole succinate min-1 mg-1 membrane protein. pa ...19892514338
proteolytic specificity of the neutral zinc proteinase from bacillus mesentericus strain 76 determined by digestion of an alpha-globin chain.the proteolytic specificity of the neutral zinc proteinase from bacillus mesentericus strain 76 (mcp 76)/bacillus subtilis was determined by using the alpha-chain of walrus hemoglobin as substrate. the resulting peptides were fractionated by gel filtration and than isolated by reversed-phase hplc. the peptides were identified on the basis of their amino-acid compositions and aligned with the known sequence of the walrus alpha-chain. the proteolytic specificity of mcp 76, deduced from the experim ...19892514721
[expression of the bacillus pumilus chloramphenicol acetyltransferase gene in bacillus subtilis, achieved by the p-r-promotor of phage lambda].the possibility of expression of the bacillus pumilus chloramphenicol acetyltransferase gene (cat) in bacillus subtilis from the pr promoter of phage lambda has been investigated in this work. for this purpose, the plasmid ppl703 carrying the b. pumilus dna segment with the cat gene lacking promoter has been combined with the plasmid pbm21 containing the pr promoter. the recombinant plasmid pel1 is capable of providing the 60 mkg/ml chloramphenicol resistance in bac. subtilis cells.19892527182
[determination of the substrate specificity of bpu101 restrictase with an unusual recognition segment].a new enzyme bpu10i was isolated from bacillus pumilus. this enzyme is not an isoschizomer of any known restriction endonucleases. the search of possible recognition sequences was carried out in sequences abcnidef (i = 0.6) on substrate dna lambda ci857, t7, pbr322. the recognition sequence and cleavage sites of restriction endonuclease bpu10i have been determined as cctnagc. ggantcg19892586500
[study on bacillus pumilus as a recipient strain for genetic engineering of bacillus].bacillus pumilus 289 can be transformed easily by plasmid pub110 through protoplast transformation with the frequency of 10(-5)--10(-3), similar to b. subtilis as 1.1176, a derivative strain of b. subtilis 168. the regeneration frequency of its protoplast is only slightly lower than b. subtilis as1.1176 (0.3-12.0% compare to 1.53--24.16%). plasmid pub110 can be maintained in both bacterial strains stably. the frequency of loss of the plasmid in both strains is lower than 3% after 45 generations ...19892629915
[thrombolytic activity of bacillus mesentericus at different conditions of nitrogen nutrition of a culture].the work is concerned with studying the effect exerted by different sources of nitrogen nutrition on the biosynthesis of proteinases with a thrombolytic activity by a variant of bacillus mesentericus, strain 64, obtained with the aid of analytical selection. protein substrates taken as a nitrogen source stimulate the synthesis of proteinases by the bacterial culture. these enzymes have a high caseinolytic and thrombolytic activity, and the level of their activity correlates with the amount of a ...19892700086
[optimization of the nutrient medium composition for directed biosynthesis of bacillus mesentericus lectins].the medium has been optimized in order to increase the lectin-producing activity of bacillus mesentericus. when a producer grew on the optimized medium, a 4-8-fold increase of the hemagglutination test titres and a 5.6-fold increase (as compared with the initial medium) of the specific lectin activity of the culture liquid was attained.19892796784
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