| structure and function of l-lactate dehydrogenases from thermophilic, mesophilic and psychrophilic bacteria, ix. identification, isolation and nucleotide sequence of two l-lactate dehydrogenase genes of the psychrophilic bacterium bacillus psychrosaccharolyticus. | two genes encoding for l-lactate dehydrogenase (ldh) from the psychrophilic bacterium bacillus psychrosaccharolyticus (dsm 6) were cloned and their nucleotide sequence determined using a pembl vector and gene hybridization probes. the deduced amino-acid sequence of the gene from clone pldh(x), which is located on a 5.87-kb hindiii-fragment, shows an identity of 86% as compared with the sequence of the wildtype ldh(p) from b. psychrosaccharolyticus and consists of 319 amino acids. clone pldh(p) c ... | 1990 | 2334516 |
| a numerical classification of the genus bacillus. | three hundred and sixty-eight strains of aerobic, endospore-forming bacteria which included type and reference cultures of bacillus and environmental isolates were studied. overall similarities of these strains for 118 unit characters were determined by the ssm, sj and dp coefficients and clustering achieved using the upgma algorithm. test error was within acceptable limits. six cluster-groups were defined at 70% ssm, which corresponded to 69% sp and 48-57% sj. groupings obtained with the three ... | 1988 | 3246588 |
| the primary structure of the psychrophilic lactate dehydrogenase from bacillus psychrosaccharolyticus. | l-lactate dehydrogenase of the psychrophilic bacterium b. psychrosaccharolyticus was isolated by a three-step procedure and its total amino-acid sequence determined by automated edman degradation. the protein consists of 318 amino-acid residues and its calculated molecular mass is 35,254 da. most of the primary structure could be established by sequencing large peptide fragments obtained by chemical cleavages, namely with bnps-skatole and with cnbr. further fragmentations of two tryptophan pepti ... | 1987 | 3435642 |
| characterization of psychrophilic alanine racemase from bacillus psychrosaccharolyticus. | a psychrophilic alanine racemase gene from bacillus psychrosaccharolyticus was cloned and expressed in escherichia coli solr with a plasmid pyok3. the gene starting with the unusual initiation codon gtg showed higher preference for codons ending in a or t. the enzyme purified to homogeneity showed the high catalytic activity even at 0 degrees c and was extremely labile over 35 degrees c. the enzyme was found to have a markedly large km value (5.0 microm) for the pyridoxal 5'-phosphate (plp) cofa ... | 1999 | 10080917 |
| affinity isolation of a cold-adapted enzyme: lactate dehydrogenase from bacillus psychrosaccharolyticus. | a simple, economical and rapid affinity chromatography procedure with dyes as the ligand has been described for the one-step purification of a cold-adapted lactate dehydrogenase. non-specific elution of procion blue h-erd-modified sepharose yielded homogeneous preparations of lactate dehydrogenase both in column based procedures and in batch wise operations. low operational temperatures resulted in the enhanced binding of the enzyme to the blue dye. the dissociation constants of the enzyme-dye c ... | 1999 | 10643641 |
| high catalytic activity of alanine racemase from psychrophilic bacillus psychrosaccharolyticus at high temperatures in the presence of pyridoxal 5'-phosphate. | we examined the effect of the pyridoxal 5'-phosphate (plp) cofactor on the activity and stability of the psychrophilic alanine racemase, having a high catalytic activity at low temperature, from bacillus psychrosaccharolyticus at high temperatures. the decrease in the enzyme activity at incubation temperatures over 40 degrees c was consistent with the decrease in the amount of bound plp. unfolding of the enzyme at temperatures above 40 degrees c was suppressed in the presence of plp. in the pres ... | 2000 | 11064190 |
| subunit interaction of monomeric alanine racemases from four shigella species in catalytic reaction. | bacterial alanine racemases are classified into two types of subunit structure (monomer and homodimer). to clarify the catalytic unit of monomeric alanine racemases, we examined the apparent molecular mass of the monomeric alanine racemases from shigella dysenteriae, shigella boydii, shigella flexneri, and shigella sonnei by gel filtration in the presence of the substrate and inhibitor. the enzymes were eluted on gel filtration as a monomer of about 39,000 da at low protein concentration and in ... | 2003 | 12725937 |
| phylogenetic relationships between bacillus species and related genera inferred from comparison of 3' end 16s rdna and 5' end 16s-23s its nucleotide sequences. | the nucleotide sequences of the 3' end of the 16s rdna and the 16s-23s internal transcribed spacer (its) of 40 bacillaceae species were determined. these included 21 bacillus, 9 paenibacillus, 6 brevibacillus, 2 geobacillus, 1 marinibacillus and 1 virgibacillus species. comparative sequence analysis of a 220 bp region covering a highly conserved 150 bp sequence located at the 3' end of the 16s rrna coding region and a conserved 70 bp sequence located at the 5' end of the 16s-23s its of the 40 sp ... | 2003 | 12807189 |
| psychrophilicity of bacillus psychrosaccharolyticus: a proteomic study. | psychrophilicity of gram-positive bacterium, bacillus psychrosaccharolyticus was investigated in a proteomic approach. one hundred and thirty-one protein spots were analyzed by electrospray ionization-quadrupole-time of flight-tandem mass spectrometry and identified using an unpublished translated contig database as well as a nonredundant gram-positive bacteria protein database from ncbi because of the lack of a genome sequence of this organism. results focused on proteomic behavior of cold-resp ... | 2004 | 15529406 |
| bacillus asahii sp. nov., a novel bacterium isolated from soil with the ability to deodorize the bad smell generated from short-chain fatty acids. | in a screening campaign to isolate strains with the ability to remove the bad smell associated with animal faeces, strain ma001(t) was isolated from a soil sample obtained from shizuoka prefecture, japan. the isolate grew at ph 6-9 but not at ph 10. cells were gram-positive, straight rods with peritrichous flagella and produced ellipsoidal spores. the isolate was positive for catalase and oxidase tests but negative for indole production, deamination of phenylalanine and h(2)s production. the iso ... | 2004 | 15545424 |
| effect of ethyl alcohol on growth and intracellular alanine racemase of psychrotrophs. | the psychrotrophic alanine racemase from pseudomonas fluorescens, a typical psychrotroph, is less resistant to organic solvents than the enzymes from thermophilic and mesophilic bacteria (okubo et al., j. home econ. jpn., 46: 1135-1140, 1995). to further elucidate this difference, we examined the effect of ethyl alcohol on the growth and intracellular alanine racemase activity of three typical psychrotrophs-p. fluorescens, bacillus psychrosaccharolyticus and b. psychrophilus-in comparison with t ... | 1999 | 16232459 |
| functional characterization of hsp33 protein from bacillus psychrosaccharolyticus; additional function of hsp33 on resistance to solvent stress. | psychrophiles have been known as efficient organism to degrade organic solvent. to investigate the mechanism of solvent stress and identify the factors that affect the solvent stress in psychrophiles, we selected bacillus psychrosaccharolyticus one of the psychrophiles and two-dimensional gel electrophoresis was performed. among the protein spots analyzed by 2-de, five spots induced in 3% ipa stress conditions were identified by ms/ms, and one of these spots was identified as a hsp33 family. the ... | 2007 | 17512907 |
| low-temperature synthesis of 2'-deoxyadenosine using immobilized psychrotrophic microorganisms. | selective biocatalyzed synthesis of 2'-deoxyadenosine from 2'-deoxypyrimidine nucleosides was carried out using free or immobilized whole cells. the reaction was performed at 57 degrees c without secondary reactions. two psychrotrophic microorganisms, bacillus psychrosaccharolyticus and psychrobacter immobilis, are described for the first time as active and specific strains for the synthesis of 2'-deoxyadenosine. adenosine deaminase activity was not detected. whole cells were immobilized in diff ... | 2008 | 18098315 |
| bacillus huizhouensis sp. nov., isolated from a paddy field soil. | a gram-stain positive, facultative aerobic bacterium, designated as strain gss03(t), was isolated from a paddy field soil. the cells were observed to be endospore forming, rod-shaped and motile with flagella. the organism was found to grow optimally at 35 °c at ph 7.0 and in the presence of 1 % nacl. the strain was classified as a novel taxon within the genus bacillus on the basis of phenotypic and phylogenetic analyses. the closest phylogenetic relatives were identified as bacillus psychrosacch ... | 2014 | 24903955 |
| bacillus gossypii sp. nov., isolated from the stem of gossypium hirsutum. | a gram-stain-positive, facultatively anaerobic, endospore-forming organism, isolated from the stem of gossypium hirsutum, was studied to determine its taxonomic position. on the basis of 16s rrna gene sequence similarity comparisons, strain jm-267t was grouped in the genus bacillus, related most closely to the type strains of bacillus simplex and bacillus huizhouensis (both 97.8%), bacillus muralis (97.7%), bacillus butanolivorans and bacillus psychrosaccharolyticus (both 97.3%). 16s rrna gene s ... | 2015 | 26303844 |
| genome of the psychrophilic bacterium bacillus psychrosaccharolyticus, a potential source of 2'-deoxyribosyltransferase for industrial nucleoside synthesis. | here we report the draft genome sequence of bacillus psychrosaccharolyticus, a cold-adapted bacterium with biotechnological interest. the genome contains genes related to the ability of this microorganism to grow at low temperatures and includes a nucleoside 2'-deoxyribosyltransferase, which can be used in the industrial synthesis of modified nucleosides with therapeutic activity. | 2013 | 23723405 |
| engineering clostridium acetobutylicum for production of kerosene and diesel blendstock precursors. | processes for the biotechnological production of kerosene and diesel blendstocks are often economically unattractive due to low yields and product titers. recently, clostridium acetobutylicum fermentation products acetone, butanol, and ethanol (abe) were shown to serve as precursors for catalytic upgrading to higher chain-length molecules that can be used as fuel substitutes. to produce suitable kerosene and diesel blendstocks, the butanol:acetone ratio of fermentation products needs to be incre ... | 2014 | 25046159 |
| nucleoside 2'-deoxyribosyltransferase from psychrophilic bacterium bacillus psychrosaccharolyticus--preparation of an immobilized biocatalyst for the enzymatic synthesis of therapeutic nucleosides. | nucleoside 2'-deoxyribosyltransferase (ndt) from the psychrophilic bacterium bacillus psychrosaccharolyticus cect 4074 has been cloned and produced for the first time. a preliminary characterization of the recombinant protein indicates that the enzyme is an ndt type ii since it catalyzes the transfer of 2'-deoxyribose between purines and pyrimidines. the enzyme (bpndt) displays a high activity and stability in a broad range of ph and temperature. in addition, different approaches for the immobil ... | 2014 | 25090115 |