| cela from bacillus lautus pl236 encodes a novel cellulose-binding endo-beta-1,4-glucanase. | cela from the cellulolytic bacterium bacillus lautus pl236 encodes eg-a, an endo-beta-1,4-glucanase. an open reading frame of 2,100 bp preceded by a ribosome-binding site encodes a protein with a molecular mass of 76,863 da with a typical signal sequence. the nh2-terminal active domain of eg-a is not homologous to any reported cellulase or xylanase and may represent a new family of such enzymes. a 150-amino-acid cooh-terminal peptide is homologous to noncatalytic domains in several other cellula ... | 1992 | 1592807 |
| multiple endo-beta-1,4-glucanase-encoding genes from bacillus lautus pl236 and characterization of the celb gene. | a bacillus lautus strain was isolated from compost by its ability to degrade microcrystalline avicel cellulose and acid-swollen cellulose. three dna fragments cloned in escherichia coli encoded at least four endo-beta-1,4-glucanases (eg), of which at least two were contained on one dna fragment. another fragment, of 2.5 kb and carrying celb, was cloned in the shuttle-vector plasmid, pjkk3-1, and expressed in e. coli and bacillus subtilis. the fragment was sequenced and shown to encode a 62-kda p ... | 1990 | 2227426 |
| stereochemistry, specificity and kinetics of the hydrolysis of reduced cellodextrins by nine cellulases. | the catalytic activity of nine enzymes (endoglucanases i-iii, v, vi and cellobiohydrolases i and ii from humicola insolens; endoglucanases a and c from bacillus lautus), representative of cellulase families a-c, h, j and k, has been investigated using a series of reduced cellooligosaccharides (cellotriitol to cellohexaitol) as substrates. for each enzyme, the specificity of cleavage was determined by analytical hplc while the kinetic constants were obtained from a kinetic assay involving a cello ... | 1993 | 8223652 |
| a polyphasic reassessment of the genus paenibacillus, reclassification of bacillus lautus (nakamura 1984) as paenibacillus lautus comb. nov. and of bacillus peoriae (montefusco et al. 1993) as paenibacillus peoriae comb. nov., and emended descriptions of p. lautus and of p. peoriae. | seventy-seven strains representing 10 species in the paenibacillus polymyxa 16s rrna group and 3 other species that exhibit phenetic relatedness to members of this group, bacillus lautus, "bacillus longisporus," and bacillus peoriae, were characterized genotypically and phenotypically by performing an amplified ribosomal dna restriction analysis, a randomly amplified polymorphic dna analysis, a fatty acid methyl ester analysis, sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-c ... | 1996 | 8863428 |
| emended description of paenibacillus amylolyticus and description of paenibacillus illinoisensis sp. nov. and paenibacillus chibensis sp. nov. | the taxonomic position of unidentified group 6 of bacillus circulans as described by nakamura and swezey (l.k. nakamura and j. swezey, int. j. syst. bacteriol. 33:46-52, 1983) was determined, and the taxonomy of paenibacillus amylolyticus was reexamined. the results of pcr amplification of a 16s rrna gene fragment with a specific primer and comparative analysis of 16s rrna gene sequences warranted placing the two taxa in the genus paenibacillus. the levels of dna reassociation among the strains ... | 1997 | 9103613 |
| paenibacillus lactis sp. nov., isolated from raw and heat-treated milk. | endospore-forming bacteria were recovered from individual packages from different processing lines in a dairy plant during a tenacious periodical contamination of their uht-milk production. two colony types were seen, one of which was identified as bacillus sporothermodurans. analysis of the 16s rrna gene of the second colony type placed these isolates within the genus paenibacillus, with paenibacillus lautus as the closest known relative. moreover, over 99 % similarity was observed to the 16s r ... | 2004 | 15143040 |
| characterization of a diesel sludge microbial consortia for bioremediation. | the organisms found growing in a mixed diesel sludge-chromium metal waste were characterized directly using environmental scanning electron microscopy (esem) and traditional fixation techniques with scanning electron microscopy (sem). an attempt to identify organisms genetically directly from the sludge failed because of interference from chemicals in the waste with polymerase chain reaction (pcr). the organisms were isolated using plate culture techniques and isolates were characterized by sem ... | 2005 | 15712752 |
| endoglucanases from paenibacillus spp. form a new clan in glycoside hydrolase family 5. | endoglucanase (egl)-producing bacteria from soil samples were screened using insoluble cellulosic substrates as sole carbon sources at alkaline ph (ph 9-10). four egls with avicelase activity at alkaline ph were found in the culture broth of each isolate. the egl genes of the isolates (all paenibacillus spp.) were shotgun cloned and sequenced-all had a 1752bp open reading frame (584 amino acids) with a putative signal sequence (33 amino acids), and encoded mature enzymes of 551 amino acids (58,3 ... | 2007 | 17368606 |
| complete genome sequence of paenibacillus strain y4.12mc10, a novel paenibacillus lautus strain isolated from obsidian hot spring in yellowstone national park. | paenibacillus sp.y412mc10 was one of a number of organisms isolated from obsidian hot spring, yellowstone national park, montana, usa under permit from the national park service. the isolate was initially classified as a geobacillus sp. y412mc10 based on its isolation conditions and similarity to other organisms isolated from hot springs at yellowstone national park. comparison of 16 s rrna sequences within the bacillales indicated that geobacillus sp.y412mc10 clustered with paenibacillus specie ... | 2012 | 23408395 |
| a novel carboxyl-terminal protease derived from paenibacillus lautus chn26 exhibiting high activities at multiple sites of substrates. | carboxyl-terminal protease (ctpa) plays essential functions in posttranslational protein processing in prokaryotic and eukaryotic cells. to date, only a few bacterial ctpa genes have been characterized. here we cloned and characterized a novel ctpa. the encoding gene, ctpap (ctpa of paenibacillus lautus), was derived from p. lautus chn26, a gram-positive bacterium isolated by functional screening. recombinant protein was obtained from protein over-expression in escherichia coli and the biochemic ... | 2013 | 24161150 |
| biochemical properties of ectoine hydroxylases from extremophiles and their wider taxonomic distribution among microorganisms. | ectoine and hydroxyectoine are well-recognized members of the compatible solutes and are widely employed by microorganisms as osmostress protectants. the ectabc enzymes catalyze the synthesis of ectoine from the precursor l-aspartate-β-semialdehyde. a subgroup of the ectoine producers can convert ectoine into 5-hydroxyectoine through a region-selective and stereospecific hydroxylation reaction. this compatible solute possesses stress-protective and function-preserving properties different from t ... | 2014 | 24714029 |
| identification and pathogenic potential of clinical bacillus and paenibacillus isolates. | the soil-related bacillus and paenibacillus species have increasingly been implicated in various human diseases. nevertheless, their identification still poses problems in the clinical microbiology laboratory and, with the exception of bacillus anthracis and bacillus cereus, little is known on their pathogenicity for humans. in this study, we evaluated the use of matrix-assisted laser desorption-ionization time of flight mass spectrometry (maldi-tof ms) in the identification of clinical isolates ... | 2016 | 27031639 |
| isolation and characterization of chitinase-producing bacillus and paenibacillus strains from salted and fermented shrimp, acetes japonicus. | chitinases catalyze the conversion of chitin and are produced by a wide range of bacteria. the biological applications of these enzymes have been exploited in food and pharmaceutical industries. we isolated 2 halophilic chitinase-producing novel strains of bacteria-sch-1 and sch-2 from saeu-jeot, a traditional korean salted and fermented food made with shrimp (acetes japonicus). the isolated strains- sch-1 and sch-2 were gram-positive, rod-shaped, endospore-forming facultative anaerobes, with st ... | 2014 | 24611959 |
| experimental investigation of microbiologically influenced corrosion of selected steels in sugarcane juice environment. | in the current study, ferritic stainless grades aisi 439 and aisi 444 were investigated as possible construction materials for machinery and equipment in the cane-sugar industry. their performance in corrosive cane-sugar juice environment was compared with the presently used low carbon steel aisi 1010 and austenitic stainless steel aisi 304. the tafel plot electrochemical technique was used to evaluate general corrosion performance. microbiologically influenced corrosion (mic) behaviour in sugar ... | 2013 | 23764955 |
| paenibacillus solani sp. nov., isolated from potato rhizosphere soil. | a novel gram-stain-positive, endospore-forming bacterium, designated fjat-22460t, was isolated from a soil sample of a potato field in xinjiang autonomous region, china. cells were rods that were catalase-positive and motile by peritrichous flagella. the strain was found to grow at temperatures ranging from 10 to 40 °c (optimum 30 °c) and at ph 5.0-12.0 (optimum ph 7) with 0-5 % (w/v) nacl (optimum 0 % nacl). phylogenetic analysis based on 16s rrna gene sequences indicated that strain fjat-22460 ... | 2016 | 27498790 |
| paenibacillus zeae sp. nov., isolated from maize (zea mays l.) seeds. | four gram-stain-positive bacterial strains, designated 6r2t, 6r18, 3t2 and 3t10, isolated from seeds of hybrid maize (zea mays l., jingke 968) were investigated using a polyphasic taxonomic approach. cells were aerobic, motile, spore-forming and rod-shaped. phylogenetic analysis based on 16s rrna gene sequences indicated that the isolates may represent a novel species of the genus paenibacillus, the four closest neighbours being paenibacillus lautus nrrl nrs-666t (97.1 % similarity), paenibacill ... | 2015 | 26373452 |
| paenibacillus qingshengii sp. nov., isolated from a lead-zinc tailing. | a novel bacterial strain, s1-9(t), was isolated from a lead-zinc tailing in nanjing, jiangsu province, china. cells of strain s1-9(t) were gram-stain-negative, ellipsoidal endospore-forming, aerobic rods and motile by means of peritrichous flagella. on the basis of 16s rrna gene sequence analysis, strain s1-9(t) was shown to belong to the genus paenibacillus and the closest phylogenetic relatives were paenibacillus glucanolyticus dsm 5162(t) (96.8% similarity), paenibacillus lautus nrrl nrs-666( ... | 2015 | 25858246 |
| paenibacillus lemnae sp. nov., an endophytic bacterium of duckweed (lemna aequinoctialis). | a gram-stain-variable, rod-shaped and endospore-forming bacterium, designated strain l7-75, was isolated from duckweed (lemna aequinoctialis). cells were motile with a monopolar flagellum. phylogenetic analysis of the 16s rrna gene sequence indicated that strain l7-75(t) belonged to the genus paenibacillus, and the closest phylogenetically related species were paenibacillus uliginis n3/975(t) (98.5% 16s rrna gene sequence similarity), paenibacillus purispatii es_m17(t) (98.5%), paenibacillus lac ... | 2015 | 25288280 |
| phenotypic switching in biofilm-forming marine bacterium paenibacillus lautus ne3b01. | biofilm-forming marine bacterium paenibacillus lautus ne3b01 was isolated from a mangrove ecosystem, odisha, india. this isolate formed a swarming type of colony pattern on the solid culture medium with 0.5-2 % agar. phase contrast microscopy study of a growing colony of p. lautus on solid media and swarming pattern revealed the existence of two phenotypically distinct cells (i.e. cocci and rods) across the colonies. however, in actively growing planktonic culture, only rod-shaped cells were obs ... | 2014 | 24452426 |