| [ribonuclease of bacillus intermedius 7 p. purification by chromatography on phosphocellulose and several characteristics of the homogeneous enzyme]. | a new procedure for isolation of homogenous ribonuclease of bac. intermedius from a commercial source is described. the yields of 140 mg of rnase from 200 g of the enzymic powder were attained. the amino acid composition of the enzyme was determined. the rnase contains neither the sulfhydryl groups nor the disulfide bonds and has only one histidine residue. at the same time the amount of aromatic amino acid residues is relatively high. the enzyme is highly resistant to heat and acid treatment bu ... | 1979 | 35250 |
| primary structure of ribonuclease from bacillus intermedius 7p. | | 1979 | 761620 |
| [the mechanisms of the manifestation of the genotoxic effects of binase]. | the high concentrations of commercial sample of bacillus intermedius 7p ribonuclease (binase) showed a weak mutagenic effect in ames test and ara-test. it has been established that binase induces the sos-functions of microbial cell and prophage induction. the way of exogenic enzyme action through activation of reca protein is proposed. | 1992 | 1302497 |
| [the cloning and expression of the rnase structural gene of bacillus intermedius]. | the structure gene of extracellular alkaline ribonuclease bacillus intermedius (binase) has been cloned in e. coli cells in composition of pmt 316 plasmid carrying the inhibitor gene (barstar of barnase--binase structure homologue. the possibility to use such vector has been proved during the barstar action on binase catalytic activity. using biochemical immunochemical analysis the expression of binase gene in e. coli cells has been confirmed. the recombinant clone e. coli which contains both pl ... | 1992 | 1302502 |
| [the isolation of highly purified bacillary ribonucleases from recombinant strains of escherichia coli]. | active and inactive highly purified extracellular alkaline ribonucleases (barnase) from recombinant escherichia coli have been prepared according to the industrial technology of bacillus intermedius ribonuclease purification. electrophoretic parameters and ultraviolet spectra characterize these preparations as homogeneous proteins. immunochemical test system of identification of inactive rnase for its purification has been worked out. | 1992 | 1302509 |
| [the cytomorphological evaluation of the effect of bacillus intermedius rnase on the organs of experimental animals]. | cytomorphological changes in organs of experimental animals after rnase bacillus intermedius treatment have been studied. it has been found that rnase stimulates elements of lympho- and homopies of thymus and spleen and increases stromal reaction and functional activity of liver. the caused effects don't depend on catalytic enzyme activity. | 1992 | 1302511 |
| [the histological evaluation of the effect of bacillus intermedius rnase on the organs of immunogenesis in laboratory animals]. | histological changes in organs of immunogenesis of experimental animals after rnase bacillus intermedius treatment have been studied. the effect of rnase on organs is revealed in activation of both cell and humoral immunity and factors of nonspecific resistance at early stage of reaction. the inhibition of immunological reactions and factors of nonspecific resistance takes place at later stages of enzyme action. the caused effects don't depend on catalytic enzyme activity. | 1992 | 1302512 |
| [the effect of bacillus intermedius rnase on the multiplication of candida tropicalis yeasts]. | the effect of bacillus intermedius rnase on the reproduction of candida tropicalis and synthesis of the main biopolymers in the yeast cells. it has been found that stimulating action of the enzyme appears at the concentration of 10(-5)-10(-6) mg/ml and does not depend on the physiological state of the sowing culture. the connection between the increase of the ionic penetration and stimulation of the rna and proteins synthesis in the yeast cells subjected to the rnase action is shown. the mechani ... | 1992 | 1302515 |
| [antibiotic-resistant mutants of bacillus intermedius--producers of a number of enzymes]. | new antibiotic-resistant strains bacillus intermedius--producers of phosphohydrolase and protease have been obtained and characterized. strain s 19 is the more active producer of extracellular enzymes. autotrophic mutants obtained on its basis possess reduced activity of phosphohydrolase. | 1992 | 1338865 |
| [genotoxicity of the preparation "binase" in tests on salmonella: ames test and ara-test]. | the present work deals with mutagenicity determination of enzyme sample "binase" (bacillus intermedius ribonuclease) in microbial test-systems: ames test and ara-test. the weak mutagenic effect of "binase" high concentration was established in both tests by induction of forward ara-mutations and histidine-reverse mutations. a metabolic activation is seen to remove this effect. | 1992 | 1502733 |
| [cytomorphologic assessment of the effect of bacillus intermedius rnase on the organs of experimental animals]. | cytomorphological changes in the organs of laboratory animals after their treatment with rnase from bacillus intermedius were investigated. it was shown that the effect of rnase on the thymus and spleen was evident from stimulation of the elements of the lympho and hemopoiesis while its effect on the liver manifested itself in increased stromal response of the liver and higher functional activity of the organ. the observed effects did not depend on catalytic activity of the enzyme. | 1992 | 1530354 |
| cloning of the gene encoding rnase binase from bacillus intermedius 7p. | | 1992 | 1594455 |
| estimating the contribution of engineered surface electrostatic interactions to protein stability by using double-mutant cycles. | coulombic interactions between charges on the surface of proteins contribute to stability. it is difficult, however, to estimate their importance by protein engineering methods because mutation of one residue in an ion pair alters the energetics of many interactions in addition to the coulombic energy between the two components. we have estimated the interaction energy between two charged residues, asp-12 and arg-16, in an alpha-helix on the surface of a barnase mutant by invoking a double-mutan ... | 1990 | 2248951 |
| [isolation and study of polyadenylated rna from bacillus intermedius]. | a poly(a)+rna fraction was isolated from the overall rna of bacillus intermedius using chromatography on poly(u) sepharose and was shown to be electrophoretically heterogeneous. the presence of a polyadenylate segment was confirmed by hybridization with polyuridine. the biological activity of the poly(a)+rna was proved by the translation in xenopus laevis oocytes. the dynamics of poly(a)+rna synthesis was studied in the course of b. intermedius growth and the content of poly(a)+rna was assayed i ... | 1988 | 2460725 |
| [isolation of mrna from protoplasts of spore-forming bacteria and study of its translation products]. | total rna was isolated from the protoplasts of bacillus intermedius 7p which actively produced alkaline exocellular rnase. a fraction of polyadenylated rna was isolated from this rna using chromatography on poly(u) sepharose. the total rna and poly(a) +rna were translated in xenopus laevis oocytes. bacterial exocellular rnase was identified among the products of translation by means of electrophoretic and immunological analyses. the mrna of rnase secreted by b. intermedius 7p was concluded to be ... | 1989 | 2479813 |
| [localization of energy domains in bacillus intermedius 7p ribonuclease]. | two independently melting regions (energetic domains) were localized in bacillus intermedius 7p ribonuclease by methods of circular dichroism and high resolution x-ray analysis: the lov-temperature melting domain, containing c-terminal region of the molecule with five strands in antiparallel beta-structure and the high-temperature melting alpha-helical domain in the n-terminal region. the contact between these domains is stabilized mainly by ionic interaction asp-22 - lys+-48. at ph 2.4 and 30.5 ... | 1989 | 2608046 |
| [pharmacokinetic properties of pancreatic and microbial ribonucleases]. | pharmacokinetic properties of pancreatic rnaase (rnaase i), rnaase of bacillus intermedius (rnaase bi) and rnaase of streptomyces rimosus (rnaase sr) were studied on albino rats. rnaase bi was shown to be characterized by a higher rate and level of absorption into the systemic blood flow, higher retention time, lower elimination from the kidneys and tissues of the peripheral chamber (skeletal muscles) and higher distribution in the other animal organs such as the heart, spleen and brain. it was ... | 1989 | 2619406 |
| [the cloning and determination of the nucleotide sequence of the rnase gene in bacillus intermedius]. | | 1989 | 2632211 |
| [in vitro and in vivo studies of rnase of bacillus intermedius]. | cytotoxicity of rnaase from bacillus intermedius was studied in vitro and in vivo. it was shown that the enzyme had slightly pronounced cytotoxicity according to the tests with inhibition of cell proliferation and biosynthesis of cell nucleic acids. the rnaase was also shown to impair the vital staining by neutral red. the efficiency of the impairment much more depended on the enzyme catalytic activity than on the proliferation and biosynthesis of nucleic acids. in vivo toxicity of rnaase from b ... | 1989 | 2751384 |
| [influence of bacillus intermedius rnaase on thymus cell composition and t-lymphocyte differentiation in an experiment]. | it was shown that exposure of mice akr and (cba x c57b1/6)f1 to bacillus intermedius rnaase and its derivative selectively inactivated by the active centre histidine stimulated t-lymphocyte maturation. with using bone marrow and spleen lymphocytes of congenitally thymus-free mice it was revealed that this effect of rnaase was defined by induction of thy-1 antigen of the lymphocyte plasmic membrane and not associated with homology of the enzyme primary structure and the alpha 1-thymosin structure ... | 1989 | 2786396 |
| [stepwise synthesis of oligonucleotides. xxxiv. preparative synthesis of trinucleoside diphosphates and longer oligoribonucleotides using immobilized ribonucleases]. | immobilized guanyl-specific ribonucleases from aspergillus clavatus (c2), a. oryzae (t1), and bacillus intermedius 7p (bi) have been used for preparative synthesis of ten trinucleoside diphosphates, three tetra- and one pentanucleotide having the only guanylic acid residue at the 5'-end. the nucleotide sequence of the oligonucleotide synthesised determined the choice of the ribonuclease. | 1987 | 3118885 |
| [an intracellular inhibitory activity of rnase secreted by bacillus intermedius]. | bacillus intermedius cells producing extracellular rnase were found to contain its inhibitor and an rnase-inhibitor complex. bacillus subtilis and escherichia coli cell lysates did not inhibit the activity of homogeneous extracellular rnase produced by b. intermedius. the inhibitor was shown to be specific for this rnase and did not interact with other rnases. as was demonstrated by biochemical tests and electrophoretic analysis, the inhibitor is released when the protoplasts are disintegrated, ... | 1988 | 3145390 |
| [modeling of the structure of a complex of ribonuclease from bacillus intermedius 7p with guanosine-3'-phosphate]. | | 1988 | 3147884 |
| distribution of charges in bacillus intermedius 7p ribonuclease determines the number of cooperatively melting regions of the globule. | a correlation between the distribution of charged side groups in the globule of bacillus intermedius 7p ribonuclease (binase) and the process of heat denaturation was studied at different ph values in order to estimate a relation between charge distribution in globular proteins and the character of cooperative thermodynamic transitions. as was shown by comparing the results of scanning microcalorimetric analysis of heat denaturation with the three-dimensional structure of binase, at optimal ph t ... | 1987 | 3270531 |
| [comparative study of the antiviral activity of pancreatic and microbial rnase]. | the efficacy of pancreatic rnase with microbial enzymes (rn-ases) of act. rimosus and bacillus intermedius) was studied comparatively in vitro in a transplantable cell culture of the swine embryokidney with respect to the aphthosa virus (av) and the virus of the aujeszky disease (vad). the vad proved to be most sensitive to rnases. rnase of bac. intermedius showed the highest antiviral efficacy. the enzymes were active in vivo, when the albino mice and newborn rabbits were infected with the av, ... | 1981 | 6267996 |
| three-dimensional structure of ribonuclease from bacillus intermedius 7p at 3.2 a resolution. | | 1983 | 6413257 |
| [intracellular and extracellular ribonucleases of bacillus intermedius]. | endocellular and exocellular ribonucleases were studied in bacillus intermedius. two fractions of ribonucleases (rf 0.72 and 0.96) were found to be associated with the cellular surface and seven fractions (rf 0.1, 0.17, 0.33, 0.45, 0.72, 0.82 and 0.96) were detected in the cytoplasm. rnaase with rf 0,096 had the highest activity and was repressed by inorganic orthophosphate. this rnase accumulated in the cell during the stationary growth phase just as the free enzyme form did in the culture medi ... | 1984 | 6482750 |
| [effect of chloramphenicol and actinomycin d on extracellular alkaline rnase biosynthesis by bacillus intermedius]. | by means of chloramphenicol it was found that biosynthesis of alkaline exocellular rnaase was repressed in bacillus intermedius by inorganic phosphate. actinomycin d at a low concentration stimulates rnaase biosynthesis in a medium with a minimal phosphorus concentration in model experiments with washed cells and in the batch culture. as a result, the activity of rnaase rises 2-4 times. the stimulating effect of actinomycin d decreases when phosphorus concentration in the medium is increased the ... | 1984 | 6513819 |
| [isolation and purification of alkaline ribonuclease from bacillus intermedius]. | the paper describes a method for preparing extracellular alkaline ribonuclease from bacillus intermedius (ec 3.1.4.23). the method consists of acid treatment of the culture fluid for selective inactivation of the interfering enzymes, concentration of the enzymic protein by ammonium sulfate precipitation, dialysis against water, chromatography on deae-cellulose and phosphocellulose in the steady state, rechromatography on a phosphocellulose containing column, desalting and freeze-drying of the en ... | 1981 | 7243746 |
| [optimization of bacillus intermedius cultivation conditions to increase biosynthesis of alkaline extracellular rnaase]. | the effect of changes in the concentrations of peptone and glucose in the growth medium, the level of aeration and the original ph value on the biosynthesis of rnaase by bacillus intermedius 7p was studied by means of a multifactor experiment. the optimal medium for the enzyme accumulation contains from 1.2 to 1.4% of glucose and from 2.8 to 3.1% of peptone, and is characterized by the original ph value of 8.45 to 8.7 and the aeration level of 1 : 7.5. the original ph value of the medium that is ... | 1980 | 7442568 |
| [regulation of biosynthesis of intracellular enzymes in bacillus intermedius 3s-19]. | the addition of microelements (co2+, cu2+, zn2+, and fe2+) to a cultivation medium increased the activity of phosphomonoesterase but not of proteinase and ribonuclease. glucose and inorganic phosphate (pi) were the main factors that affected the direction and intensity of the biosynthesis of extracellular enzymes. | 1995 | 7479635 |
| bacterial ribonuclease: mutagenic effect in microbial test-systems. | pure enzyme samples of ribonuclease from bacillus intermedius 7p (known commercially as 'binase') were investigated for genotoxicity in four microbial tests: the ames plate incorporation method, arar-assay; the prophage induction test; and the dna-repair test. the weak mutagenic effect of binase at high concentrations (0.1 mg/plate, 1 mg/plate) was established by induction of forward arar-mutations and histidine-reverse mutations (both frameshift mutations and base pair substitution). metabolic ... | 1995 | 7666766 |
| [the effect of bacillus intermedius rnaase on the growth and development of bacillus mucilaginosus]. | the effect of bacillus intermedius rnaase on vegetative cells and spores of siliceons bacteria bacillus mucilaginosus was investigated. it is shown that the enzyme stimulates the growth of vegetative cells of b. mucilaginosus at a concentration of 10 mkg/ml and promotes germination of spores at a 1000-fold lesser concentration (0.1 mkg/ml). the spores at stages of activation and initiation appear to be most susceptible to the enzyme action. the effect of rnaase on b. mucilaginosus multiplication ... | 1995 | 7715481 |
| [biosynthesis of extracellular ribonuclease by bacillus pumilus]. | conditions for the biosynthesis of alkaline extracellular ribonuclease by bacillus pumilus were studied and their comparison with that of the biosynthesis of ribonuclease by bacillus intermedius was made. it was estimated that ribonuclease of bacillus pumilus is synthesized post-exponentially, its synthesis is inhibited by inorganic phosphate and is stimulated by actinomycin d, as the synthesis of ribonuclease by bacillus intermedius. at the same time essential differences in dynamic of enzyme f ... | 1994 | 7760766 |
| dissociation constants and thermal stability of complexes of bacillus intermedius rnase and the protein inhibitor of bacillus amyloliquefaciens rnase. | binase, the extracellular ribonuclease of bacillus intermedius, is inhibited by barstar, the natural protein inhibitor of the homologous rnase, barnase, of b. intermedius. the dissociation constants of the binase complexes with barstar and its double cys40,82ala mutant are about 10(-12) m, only 5 to 43 times higher than those of the barnase-barstar complex. as with barnase, the denaturation temperature of binase is raised dramatically in the complex. calorimetric studies of the formation and sta ... | 1995 | 7789535 |
| [secreted serine proteinase from the spore-forming bacteria bacillus intermedius 3-19]. | extracellular serine proteinase has been isolated from the cultural medium of bacillus intermedius 3-19 using cm-cellulose chromatography and affinity chromatography on bacitracin-sepharose. the specificity of the proteinase with a wide range of natural and synthetic substrates has been investigated. the greatest activity was observed with tripeptides containing c-terminal leu or phe. the enzyme was completely inhibited by diisopropylfluorophosphate and partly inhibited by thiol-specific reagent ... | 1994 | 7819414 |
| mutational analysis of the active site of rnase of bacillus intermedius (binase). | to elucidate the functional role of some residues in the active site of binase, the extracellular ribonuclease of bacillus intermedius, we used site-directed mutagenesis. on cleavage of various substrates the catalytic activity of binase mutant his101 glu is 2.0-2.7% of that for the native enzyme. the decrease in activity is determined mainly by the decrease in molecular rate constant kcat, with almost unchanged affinity of the enzyme for the substrate, characterized by km. this is the expected ... | 1994 | 7957945 |
| [isolation of intracellular inhibitors of bacterial rnases on a column with immobilized bacillus intermedius rnase]. | intracellular inhibitors of rnases from bacillus amyloliquefaciens and bac. intermedius were isolated using affinity chromatography on covalently immobilized rnase from bac. intermedius. the inhibitor of rnase from bac. amyloliquefaciens was isolated from cells of e. coli hb 101 and purified to homogeneity. proteins with molecular weights of 70, 36 and 20 kd possessing an inhibitory activity were found in the extract obtained from frozen cells of bac. intermedius. | 1994 | 8047536 |
| preparation of cyclic 2',3'-monophosphates of oligoadenylates (a2'p)na > p and a3'p(a2'p)n-1a > p. | the action of the guanylyl-preferring rnase from bacillus intermedius (binase) on a mixture of oligoadenylates with randomly distributed 2'-5' and 3'-5' internucleotide bonds [(a2'/3'p)n] under conditions sufficient for complete hydrolysis of poly(a) results in a mixture containing a single circular oligoadenylate and two series of linear oligoadenylates ending in cyclic 2',3'-phosphate. individual compounds formed upon digestion of (a2'/3'p)n with binase have been isolated. their structure was ... | 1994 | 8119303 |
| [interaction of bacillus intermedius 7p ribonuclease with ligand-free serum albumin]. | the efficiency of the binding of rnase 7p molecules to albumin on cocondensation with the aim of producing the prolonged action forms of the enzyme can be increased by using ligand-free human serum albumin (lfhsa). the cd method showed that lfhsa underwent changes of the cooperative character under the action of acid and urea. on potentiometric titration the number of titrated groups of lfhsa decreased with time. the gpc method demonstrated the rnase bound more efficiently to freshly dissolved l ... | 1994 | 8146114 |
| [superproduction of bacillus intermedius 7p ribonuclease (binase) in escherichia coli]. | we have reported previously about the cloning of the binase gene in e. coli. in this work, using an original approach named "homolog gene recombination" method (hgr), vectors for binase expression in e. coli have been constructed. transcription of the binase gene have been directed through either tac-promoter or pr-promoter of bacteriophage lambda under the control of temperature-sensitive ci857 repressor. the last promoter gave the maximum yield of binase, up to 100 mg of protein per litre of h ... | 1994 | 8183278 |
| [cloning of the gene for extracellular bacillus circulans rnaase]. | the gene for extracellular low molecular weight ribonuclease of bacillus circulans bcf 247 was cloned. the strain was isolated from permafrost deposits of the kolyma lowland. the gene for the ribonuclease from bacillus intermedius (binase) was used as a specific probe. the cloning succeeded only in the e. coli strain producing the inhibitor of ribonuclease form bacillus amyloliquefaciens. selected clones secreted the active ribonuclease into the growth media. deletion derivatives of the parental ... | 1994 | 8183279 |
| [alkaline extracellular proteinase from bacillus intermedius. isolation, purification, and some properties of the enzyme]. | extracellular alkaline proteinase was isolated from the bacillus intermedius culture medium. the enzyme was purified by ion-exchange chromatography 200-fold to apparent homogeneity and has a specific activity of 950 u./mg. the proteinase was maximally active at ph 10, 50 degrees c and is stable at ph 6.3-11.0. edta, o-phenanthroline or p-chloromercuribenzoate did not affect the enzyme activity, while phenylmethylsulfonyl fluoride inhibited it by 95-97%. it was concluded that the enzyme is subtil ... | 1993 | 8292652 |
| increase of specificity of rnase from bacillus amyloliquefaciens (barnase) by substitution of glu for ser57 using site-directed mutagenesis. | bacterial ribonucleases from bacillus amyloliquefaciens and bacillus intermedius show the specificity towards the nature of a nucleoside at the o3' end of the phosphodiester bond to be split in the preference order g > a >> u > c in the cleavage reactions of polynucleotides. it follows from the x-ray data that the substrate guanosine base is bound at the active site of these rnases in the same manner as for high-specificity guanylic rnases. we supposed that the difference in specificity for the ... | 1993 | 8344276 |
| [ionic pairs in bacillus intermedius 7p ribonuclease]. | on the basis of x-ray determined coordinates specified with computer graphics and new data on amino acids sequence in the structure of bacillus intermedius 7p ribonuclease there were analysed potentially charged residues and found ionic pairs binding independently denaturing regions-energetic domains. five clusters of charged amino acids were found in the protein structure and several ionic bonds between the energetic domains were discovered: asp7, asp11-arg109 and lys26-asp53,glu72. breaking of ... | 1993 | 8471643 |
| [comparison of the heat stability and structure close homologs--bacillus amyloliquefaciens ribonuclease and bacillus intermedius 7p ribonuclease]. | parameters of heat denaturation and intrinsic fluorescence of barnase and its close homologue, binase, in the ph region 2-6 have been determined. barnase heat denaturation (ph 2.8-5.5) proceeds according to the "all-or-none" principle. barnase denaturation temperature is lower than that of binase and this difference increases from 2.5 degrees c at ph 5 to 7 degrees c at ph 3. enthalpy values of barnase and binase denaturation coincide only at ph 4.5-5.5, but as the ph decreases the barnase denat ... | 1993 | 8487771 |
| [the mechanism of the antitumor action of bacillus intermedius ribonuclease]. | in a concentration providing the maximum in vivo antitumor action, "bacillus intermedius" rnaase had no in vitro cytotoxic effect on the cells of lympholeukosis nk/ly. the enzyme did not inhibit the involvement of 3h-thymidine in the dna biosynthesis in the tumor cells cultivated in vitro. a preliminary in vitro exposure of the cells to the enzyme did not influence their inoculation capacity. it was concluded that the mechanism of the enzyme antitumor action was not associated with the inhibitio ... | 1995 | 8534172 |
| [the modification of bacillus intermedius ribonuclease by urea derivatives]. | the results of the "bacillus intermedius" rnaase modification by urea derivatives are presented. the modifiers synthesized for that purpose were the following: n-(4-chlorbenzoyl)-n'-benzolsulfonyl urea (m1) and n-(4-chlorbenzoyl)carbomoyl-epsilon-aminocapronic acid (m2). it was shown that rnaase modified by m2 stimulated the cell general metabolism by the test of the cell absorption of neutral red and had a more marked ability to disrupt rna of the cell plasmatic membranes in comparison to that ... | 1995 | 8534173 |
| [effect of membranotropic physiologically-active substances on the growth of saccharomyces cerevisiae cultures]. | the effect of bacillus intermedius rnase and yeast autoregulatory d2 factor on growth of saccharomyces cerevisiae is reported. it is shown that 0.01 microgram/ml of rnase stimulates the growth of the yeast on malt wort and molasses, the peak of the effect being observed for the enzyme added in the middle exponential phase. certain concentrations of the d2 factor can also potentiate yeast growth. the effect is more pronounced if the d2 factor and rnase are added in combination rather than singly. ... | 1995 | 8538510 |
| [biosynthetic regulation of extracellular ribonucleases in native strains of bacilli and in recombinant strains of escherichia coli]. | regulation of the biosynthesis of extracellular ribonucleases of bacillus amyloliquefaciens h2 (barnase), bacillus intermedius 7p (binase), and bacillus pumilus kmm 62 (rnase bp) was studied in their native strains and recombinant escherichia coli strains. recombinant plasmids were obtained that contained genes encoding barnase, binase, and rnase bp under the control of their own regulatory sequences (plasmids pmt415, pml5, and pml61), genes encoding barnase and binase under the control of the t ... | 1995 | 8538511 |
| [biosynthesis of alkaline extracellular proteinases in bacillus intermedius]. | the conditions for growth and serine proteinase biosynthesis were studied in the batch culture of bacillus intermedius. the synthesis of the enzyme was found to be inhibited by glucose and by the amino acid mixture and activated by the addition of organic compounds-casein and gelatin-to the medium. inorganic phosphate in the medium increased the enzyme yield. the enzyme activity was shown to be twofold higher upon the addition of corn extract (1.8%) in the medium than in the medium with meat pep ... | 1995 | 8538512 |
| site-directed mutagenesis of the base recognition loop of ribonuclease from bacillus intermedius (binase). | members of the microbial guanyl-specific ribonuclease family show a high level of structural homology. the structural basis for guanyl base binding by microbial ribonucleases has been established for all members of the family and the existence of a guanine recognition loop was shown. however, bacillar rnases such as binase and barnase show far less specificity towards the guanyl base in hydrolysing oligonucleotides composed of more than 4 or 5 nucleotides. using site-directed mutagenesis we intr ... | 1996 | 8612811 |
| [ribonuclease stimulation of nonspecific factors of protection in experimental animals]. | it was shown that a single intraperitoneal administration of bacillus intermedius rnase to rats stimulated the activity of lysozyme and blood serum complement. a single intraperitoneal administration of pancreatic rnase, bacillus intermedius rnase and its derivative selectively inactivated by the histidine active centre stimulated the metabolic activation of neutrophils as was shown by their ability to reduce tetrazolium nitroblue to diphormazone. the efficiency of the neutrophil stimulation by ... | 1995 | 8645057 |
| [preparation and antiviral properties of bacillus intermedius ribonuclease modified by chloranhydride of adamantanecarboxylic acid]. | bacillus intermedius ribonuclease modified by the residue of adamantane carboxylic acid was prepared. when the cells of chick embryo fibroblasts infected by the fowl plague virus were exposed to the modified ribonuclease, the antiviral activity proved to be higher by comparison to that of the native enzyme. the chemotherapeutic index of the rnase after the modification increased 4 times. | 1995 | 8651823 |
| [effect of bacillus intermedius rnaase on growth of a saccharomyces cerevisiae culture]. | the effects of rnase from bacillus intermedius on proliferation of saccharomyces cerevisiae were studied. the enzyme (0.01 microgram/ml) stimulated the yeast cell budding. this effect was dose-dependent and required an appropriate physiological stage of the growing culture cells. rnase produced maximal effects when added to exponentially growing cultures. analysis of the age structure of the population showed that exogenous rnase stimulated the cell cycle at a stage preceding the initiation of d ... | 1996 | 8725448 |
| a novel bacillus intermedius extracellular alkaline phosphatase: isolation, physico-chemical and catalytic characteristics. | a new alkaline phosphatase was obtained as homogeneous preparation from culture filtrate of the spore-forming bacillus intermedius. b. intermedius phosphatase was shown to be monomer with molecular weight of 47 kda. the enzyme possesses phosphomonoesterase and phosphodiesterase activities and exhibits a broad specificity towards a wide variety of substrates. the purified phosphatase had an optimum temperature of 50 degrees c, optimum ph of 9.5 and was stable until 60 degrees c at ph 8-10. the ef ... | 1996 | 8728105 |
| [an immunocytochemical study of bacterial ribonuclease penetration into candida utilis yeast cells and the effect of the enzyme on yeast multiplication]. | permeability of candida utilis cells for exogenic bacillus intermedius rnase was studied cytochemically. the enzyme was shown to penetrate through the outer membrane of c. utilis after a 20 min incubation with cells being in exponential phase, and to be connected with the yeast cytoplasmic membrane. | 1996 | 8768551 |
| [modification of bacillus intermedius ribonuclease by dialdehyde dextran]. | conditions for modification of bacillus intermedius ribonuclease with dialdehyde dextran was optimized by box and wilson's method to achieve the enzyme with higher activity. the generalized parameter including the yield of enzyme protein and enzyme stability during modification was used. the yield of activity and preparation characteristics depended on a number of enzyme-carrier bonds. | 1996 | 8984473 |
| [chemical modification of rnase from bacillus intermedius. effect on the cytotoxic properties of the enzyme]. | modified derivatives of rnase from bacillus intermedius differing in the aggregate enzymatic, physico-chemical and physical properties were prepared. the cytotoxic properties of the compounds were tested by the intravital staining of fl cells with neutral red. it was shown that the native enzyme cytotoxicity correlated with the catalytic activity. however, it was found possible to lower the cytotoxicity of the catalytically active compounds by changing the enzyme aggregate properties. | 1996 | 8999766 |
| [changes in the size and morphology of cells under the influence of bacterial ribonuclease]. | it has been found that a single interperitoneal injection of mice with nkly ascite lympholeukosis cells and bacillus intermedius rnase or its derivative, obtained in result of activation of the active centre, on histidine induces an increase in size of cells cultured in vivo, the number of morphological changes in the cell cytoplasm, cell nucleus and, to a large extend, in the cell surface. a share of 2-3 nucleated cells increases as a result of both an incomplete mitosis and cell fusion. these ... | 1996 | 9005643 |
| [influence of bacillus intermedius rnase on growth-stimulating and antagonistic characteristics of trichoderma harzianum]. | bacillus intermedius rnaase (with specific activity of 1,000,000 units per one mg of protein) at concentration of 1 x 10(-3) mg/ml was shown to increase antagonistic and growth-stimulating properties of trichoderma harzianum. an application of trichodermin which was treated with an enzyme enhanced cucumber crop capacity by 15-18% in industrial conditions. | 1996 | 9011860 |
| [the stimulating action of exogenous rnase on eubiotic strains of lactobacillus, bifidobacterium and escherichia coli]. | the influence of bacillus intermedius rnaase on the multiplication on b.b.bifidum, l.fermentum and e. coli was studied. the study revealed that the stimulating action depended on the dose of the enzyme, the microbial species and the growth phase of the inoculate. rnaase, added to the nutrient medium, was shown to induce the acceleration of the synthesis of dna, rna, protein, as well as mitosis. at the same time the stimulating action of rnaase was accompanied not only by the reduction of the dur ... | 1996 | 9027166 |
| [change in various biological properties of lactobacillus as affected by ribonuclease]. | the influence of rnase from bacillus intermedius on the growth of the industrial strain lactobacillus plantarum 8r-a3 was studied. it was shown that the stimulating effect of the enzyme depended on its dose and manifested itself in decreasing the growth lag phase. at the same time the growth stimulating dose of rnase increased the lactobacillus adhesion to the epithelial cells and promoted secretion of proteinases from lactobacillus to the culture medium. the possible use of rnase as a stimulant ... | 1996 | 9027286 |
| [effect of culture medium components on the accumulation of extracellular bacillary ribonucleases in culture fluid of recombinant strains of escherichia coli]. | the effect of the concentrations of peptone, yeast extract, and inorganic phosphate on the expression of genes of extracellular ribonucleases from bacillus intermedius 7p (binase) and bacillus amyloliquefaciens h2 (barnase) was studied in escherichia coli cells transformed with plasmids containing the structural genes of binase or barnase under the control of their own or synthetic regulatory region or the structural binase gene under the control of the regulatory regions of the genes of barnase ... | 1996 | 9102546 |
| enzymatic properties of thiol-dependent serine proteinase of bacillus intermedius 3-19. | effects of a thiol-dependent serine proteinase of bacillus intermedius on peptide substrates and insulin b-chain were studied. the enzyme preferably splits peptide bonds formed by carboxyl groups of hydrophobic amino acids. ca2+ increases the thermal stability of the proteinase significantly. the kinetic characteristics of hydrolysis of z-ala-ala-leu-pna by this enzyme was determined as k(m) = 1.25 mm and kcat = 0.15 sec-1. the enzyme has high stability to dmfa and isopropanol, and is able to ca ... | 1997 | 9113729 |
| glutamyl endopeptidase of bacillus intermedius, strain 3-19. | a homogeneous glutamyl endopeptidase splitting peptide bonds of glutamic, rarely of aspartic acid residues in peptides and proteins, was isolated from bacillus intermedius 3-19 culture filtrate using chromatography on cm cellulose and mono s. the enzyme molecular mass is equal to 29 kda, pi 8.4. the protease is inhibited by diisopropylfluorophosphate. the enzyme, like other glutamyl endopeptidases, reveals two ph optima at ph 7.5 and 9.0 for casein and one at ph 8.0 for z-glu-pna hydrolysis. a 6 ... | 1997 | 9119071 |
| rna cleavage without hydrolysis. splitting the catalytic activities of binase with asn101 and thr101 mutations. | members of the microbial guanyl-specific ribonuclease family catalyse the endonucleolytic cleavage of single-stranded rna in a two-step reaction involving transesterification to form a 2',3'-cyclic phosphate and its subsequent hydrolysis to yield the respective 3'-phosphate. the extracellular ribonuclease from bacillus intermedius (binase, rnase bi) shares a common mechanism for rna hydrolysis with mammalian rnases. two catalytic residues in the active site of binase, glu72 and his101, are thoug ... | 1997 | 9153077 |
| nephrotoxic effects of bacterial ribonucleases in the isolated perfused rat kidney. | alterations of the renal function in the isolated perfused rat kidney system after application of two bacterial rnases, bacillus intermedius rnase (binase) and ribonuclease produced by bacillus amyloliquefaciens (barnase), were investigated with two different treatment regimens in comparison with catalytically inactive derivates of the enzymes, photooxidated at the active site his101 binase and inactive mutant his102gln barnase. for the in vitro approach the test enzymes were dissolved in the pe ... | 1997 | 9160109 |
| [cytotoxic properties of the complex of the antineoplastic antibiotic bleomycetin and bacillus intermedius ribonuclease]. | it was shown possible to change the cytotoxic properties of the antitumor antibiotic bleomycetin by its binding to bacillus intermedius rnase. the complexing lowered the antibiotic effect on dna in the cells of the human amnion. at the same time the experiments with human red blood cells indicated that rnase of b. intermedius in complex with bleomycetin-fe(ii) increased the antibiotic capacity for the cell membrane break down. | 1997 | 9182501 |
| glutamyl endopeptidase of bacillus intermedius strain 3-19. purification, properties, and crystallization. | a homogeneous glutamyl endopeptidase splitting peptide bonds of glutamic and, rarely, of aspartic acid residues in peptides and proteins was isolated from bacillus intermedius 3-19 culture filtrate using chromatography on cm-cellulose and mono s. the enzyme molecular mass is 29 kd and the pi is 8.4. the proteinase is inhibited by dfp. the enzyme, like other glutamyl endopeptidases, reveals two ph optima (ph 7.5 and 9.0) for casein and one (ph 8.0) for z-glu-pna hydrolysis. the k(m) for the hydro ... | 1997 | 9360302 |
| [induction of the sos-response with bacillus intermedius rnaase]. | this paper deals with the induction of the sos-functions of a bacterial cell by the exogenous ribonuclease of bacillus intermedius. two test systems were employed, which made it possible to quantitatively estimate sos-response induction activity in gram-positive and gram-negative bacteria. it was established that the catalytically active enzyme elicits the sos-response in the cells of escherichia coli pq 37 and causes prophage induction in the lysogenic strain bacillus subtilis 168 phi 105 wt (a ... | 1997 | 9379968 |
| [actinomycin d influence on biosynthesis of extracellular ribonucleases by sporulating bacteria]. | the influence of actinomycin d on the synthesis of extracellular ribonucleases by "bacillus intermedius" (binase), b.pumilus (rnase bp) and b.amyloliquefaciens (barnase) was studied comparatively. when added during the active synthesis of the enzymes actinomycin d stimulated the biosynthesis of binase and rnase bp and had no influence on the barnase biosynthesis. the response of the bacillary rnase biosynthesis to the added actinomycin d correlated with the differences in the nucleotide sequence ... | 1997 | 9463296 |
| [secondary structure of binase in solution by 1h nmr]. | nearly all resonances were assigned in the two-dimensional 1h nmr spectra of binase, guanylospecific ribonuclease from bacillus intermedius containing 109 amino acid residues. the exchange rates of amide protons with the solvent deuterium were measured in 2h2o at ph 6.7 and 30 degrees c. coupling constants 3j of h-nc alpha-h, noe contacts, solvent exchange rates of amide protons, and indices of c alpha h chemical shifts were measured, and the binase secondary structure was deduced from these dat ... | 1997 | 9490613 |
| contribution of arginine-82 and arginine-86 to catalysis of rnases from bacillus intermedius (binase). | to elucidate the functional role of arg82 and arg86 in the enzyme activity of binase, the extracellular ribonuclease of bacillus intermedius, we used site-directed mutagenesis. on cleavage of various substrates the catalytic activity of binase mutant arg86 ala is 2.7 x 10(3) - 7.7 x 10(3) times less than that of the native enzyme. the decrease in activity is determined preferentially by the decrease in the molecular rate constant kcat with a relatively small change of enzyme-substrate affinity, ... | 1998 | 9645474 |
| [effect of bacillus intermedius rnaases with various catalytic activity on multiplication of escherichia coli and bacillus subtilis]. | the effect of the bacillus intermedius ribonuclease and its mutant forms derived by site-specific mutagenesis on the growth of gram-positive and gram-negative bacteria was studied. both catalytically active and catalytically inactive (mutant) ribonucleases stimulated bacterial growth, the extent of stimulation correlating with the catalytic activity of the enzymes. it was suggested that the biological activity of exogenous ribonucleases is mainly due to their catalytic activity. | 1998 | 9662695 |
| high-resolution detection of five frequencies in a single 3d spectrum: hnhcaco--a bidirectional coherence transfer experiment. | a new triple-resonance pulse sequence, 3d hnhcaco, is introduced and discussed, which identifies sequential correlations of the backbone nuclei (h alpha (i-1), c alpha (i-1), c(i-1), nh(i), n(i)) of doubly labeled proteins in h2o. the three-dimensional (3d) method utilizes a recording of 15n and 13c resonances in a single indirect time domain, the 13c' resonance in another indirect time domain, and detects both nh and h alpha protons. a bidirectional coherence transfer (nh(i) <--> n(i) <--> c(i- ... | 1998 | 9679293 |
| effect of ribonuclease from bacillus intermedius on human blood lymphocytes. | by using a rosette formation test the effect of ribonuclease bacillus intermedius (rnase bi) on t- and b-lymphocytes in human peripheral blood has been studied in vitro. the rnase effect on t-lymphocytes depends on its concentration: low concentrations (10(-6)-10(-2) microg ml(-1)) stimulate e-rosette formation whereas high concentrations (10 microg ml(-1)) suppress it. the amount of b-lymphocytes decreases under rnase bi influence in all concentrations tested. rnase bi like thymus hormones infl ... | 1998 | 9685000 |
| [thrombolytic and anticoagulant properties of thiol-dependent proteinase from bacillus intermedius 3-19]. | | 1998 | 9703630 |
| isolation and properties of extracellular alkaline phosphatase from bacillus intermedius. | alkaline phosphatase (apase) was isolated from the culture liquid of the streptomycin-resistant strain of bacillus intermedius s3-19 and purified as a homogeneous preparation by ion-exchange chromatography and fplc. electrophoresis and gel-filtration revealed that the active enzyme is a monomer with molecular weight of 46-47 kd. the enzyme possessed phosphomonoesterase and phosphodiesterase activities with maximal levels at ph 9.5 and 55 degreesc and was stable until 60 degreesc at ph 8.0-10.0. ... | 1998 | 9864452 |
| bacillus intermedius glutamyl endopeptidase. molecular cloning and nucleotide sequence of the structural gene. | the glutamyl endopeptidase gene of bacillus intermedius was cloned from a genomic library expressed in bacillus subtilis and sequenced (embl accession number y15136). the encoded preproenzyme contains 303 amino acid residues; the mature 23-kda enzyme consists of 215 residues. the mature enzyme reveals 38% of identical residues when aligned with the glutamyl endopeptidase from bacillus licheniformis, whereas only five invariant residues were found among all known glutamyl endopeptidases. the amin ... | 1999 | 10071925 |
| [expression of secreted guanyl-specific ribonuclease genes from bacillus intermedius and bacillus pumilus in bacillus subtilis cells]. | plasmids with whole genes for ribonucleases from b. intermedius (binase) and b. pumilis (rnase bp) assembled with the whole gene of barstar, a specific intracellular inhibitor, are constructed. the resultant plasmids pmz55 and pmz56 effectively express binase and rnase bp genes in b. subtilis cells. a medium for maximum expression of rnase genes by recombinant strains is developed. the expression of binase and rnase bp genes in b. subtilis cells is negatively regulated by exogenic inorganic phos ... | 1999 | 10190104 |
| expression of the genes for guanyl-specific ribonucleases from bacillus intermedius and bacillus pumilus is regulated by the two component signal transduction system phop-phor in b. subtilis. | promoters of the genes for guanyl-specific ribonucleases, secreted by b. intermedius (binase) and b. pumilus (rnase bp) in phosphate deficient conditions, contain regions similar to appropriate consensus sequences in promoters of the pho regulated genes of b. subtilis. a number of genes expressed in response to phosphate starvation in b. subtilis are regulated by the two component signal transduction system phop-phor. expression of recombinant genes for binase and rnase bp in b. subtilis strains ... | 1999 | 10220898 |
| [effect of the rnaase from bacillus intermedius on growth and physiological characteristics of escherichia coli]. | the effect of the rnase from bacillus intermedius on the growth of escherichia coli was investigated. rnase added to growth medium enhanced the synthesis of dna, rna, and protein and stimulated cell division; the degree of stimulation depended on the enzyme concentration. a necessary condition for stimulation was the adsorption of the enzyme on the cell surface and its interaction with the cytoplasmic membrane, as demonstrated immunocytochemically. the adsorption of the enzyme was accompanied by ... | 1999 | 10368802 |
| [changes in the trophic organization of mitotic cycle of candida utilis after exposure to rnase from bacillus intermedius]. | the effect of the rnase from bacillus intermedius on the growth and trophic cycle of candida utilis was studied. the rnase at concentrations of 0.001-0.01 microgram/ml stimulated yeast growth by 30-40% as compared to the control, reduced the mitotic cycle of the yeast by shortening its g1 phase, and decreased the number of exotrophic cells in the g1 phase to a minimum. it was suggested that rnase is involved in the regulation of the transition of cells from the exo- to endotrophic state. | 1999 | 10420396 |
| [expression of genes for guanyl-specific ribonucleases in bacillus intermedius and bacillus pumilus is regulated by the phop-phor two-component signal transduction system of pho regulon of bacillus subtilis]. | promoters of the genes of guanyl-specific ribonucleases of bacillus intermedius (binase) and bacillus pumilus (rnase bp) were found to contain sequences homologous to those recognizable by the regulatory protein phop in the promoters of the pho regulon of b. subtilis, as well as regions partially homologous to the binding sites of another regulatory protein, phob, in the promoters of the pho regulon of escherichia coli. the role of the two-component regulatory systems phop-phor and phob-phor in ... | 1999 | 10495972 |
| preparation of spin-labeled bacterial ribonuclease from bacillus intermedius 7p for epr studies of protein dynamics. | the optimal conditions for labeling of binase (a bacterial ribonuclease isolated from bacillus intermedius 7p) with a bromoacetamide spin label have been determined. the label is suitable for probing the dynamics of the protein by electron paramagnetic resonance (epr). binase samples specifically labeled at residue his-101 of the active center were prepared by incubation for 48 h at 20 degrees c in potassium phosphate buffer (ph 5.5) containing the bromoacetamide (1:3 protein/label molar ratio). ... | 1999 | 10498815 |
| biosynthesis and localization of glutamylendopeptidase from bacillus intermedius strain 3-19. | the biosynthesis of glutamylendopeptidase from bacillus intermedius strain 3-19 and localization of the enzyme in the bacterial cells was studied. the synthesis of the enzyme was suppressed by easily metabolizable carbon sources. inorganic phosphate and nh4+ ions stimulated the production of glutamylendopeptidase. complicated organic substrates such as casein, gelatine, and haemoglobin did not affect the biosynthesis of the enzyme. the divalent metallic ions ca2+, mg2+, co2+ increased the produc ... | 1999 | 10581732 |
| [the effect of carbon sources and mononucleotides on the production of extracellular alkaline phosphatase by bacillus intermedius]. | the biosynthesis of extracellular alkaline phosphatase in the streptomycin-resistant strains bacillus intermedius s3-19 and s7 in the presence in the medium of 5'-nucleoside monophosphates and different sources of carbon--glucose, sodium pyruvate, sodium lactate, or glycerol--was studied. it was established that, in the presence of mononucleotides, the content of extracellular alkaline phosphatase in both strains increased; the maximal effect was caused by 5'-amp at a concentration of 20 microgr ... | 2000 | 10776617 |
| [localization of alkaline phosphatase in cells of bacillus intermedius]. | alkaline phosphatase, an enzyme secreted by bacillus intermedius s3-19 cells to the medium, was also detected in the cell wall, membrane, and cytoplasm. the relative content of alkaline phosphatase in these cell compartments depended on the culture age and cultivation medium. the vegetative growth of b. intermedius on 0.3% lactate was characterized by increased activity of extracellular and membrane-bound phosphatases. the increase in lactate concentration to 3% did not affect the activity of me ... | 2000 | 10776618 |
| [stabilization of enzymes by anabiosis autoinducers as a possible mechanism of resistance of resting microbial forms]. | alkyl-substituted hydroxybenzenes (ahbs), auto-inducers of microbial dormancy (or d1 factors), were found to stabilize the structure of protein macromolecules, making them metabolically less active and more resistant to stresses. in vitro experiments with the bacillus intermedius ribonuclease and chymotrypsin showed that the degree of the physical and chemical stability of these enzymes treated with ahbs depends on their concentration and incubation time. experiments with rnase, which is capable ... | 2000 | 10776622 |
| [effect of culture media components on accumulation of glutaminyl endopeptidase in culture liquid of bacillus intermedius 3-19]. | the effect of nutrients and growth conditions on the accumulation of glutamyl endopeptidase in the culture liquid of bacillus intermedius 3-19 was studied. glucose and other readily metabolizable carbon sources were found to suppress the production of the enzyme, while inorganic phosphate and ammonium cations enhanced it. protein substrates, such as casein, gelatin, and hemoglobin, did not affect enzyme production. some bivalent cations (ca2+, mg2+, co2+) increased the production of glutamyl end ... | 2000 | 10808485 |
| a novel secreted ribonuclease from bacillus intermedius: gene structure and regulatory control. | a second secreted ribonuclease, designated binase ii, has been detected in bacillus intermedius 7p, and its structural gene was cloned and sequenced. unlike the well-known binase i, a 109-amino acid guanyl-specific enzyme, the 292-residue binase ii is closely related to the b. subtilis nuclease bsn, in structure and in its enzymatic properties. binase ii is also insensitive to inactivation by barstar, an inhibitor protein that is specific for guanyl-specific ribonucleases. while both b. intermed ... | 2000 | 10852477 |
| [effect of bacillus intermedius ribonuclease on properties of the yeast saccharomyces cerevisiae]. | effects of bacillus intermedius ribonuclease on physiological, biochemical, and consumer properties of baker's yeast saccharomyces cerevisiae were studied. this enzyme improved the yeast raising strength and increased the cell tolerance to various adverse factors. the antistress effect of rnase correlated with an earlier start of the stationary growth phase and increased trehalose pool. | 2000 | 10994200 |
| [dependence of the effect of rnaase from bacillus intermedius on the growth of baker's yeast on the concentration of the exogenous enzyme]. | bacillus intermedius rnase added at a low concentration (0.001 microgram/ml) stimulated yeast growth, while a high rnase concentration (1500 micrograms/ml) was inhibitory to yeast growth. the inhibitory effect of rnase was transient and correlated with the increase in the trehalose pool of yeast cells. the number of unbudded cells in the yeast population tended to decrease under the action of low concentrations of bacillar rnase and to increase under the action of high concentrations of this enz ... | 2000 | 11008682 |
| bacillus intermedius ribonuclease as inhibitor of cell proliferation and membrane current. | the antiproliferative action of the guanine-specific ribonuclease secreted by bacillus intermedius (binase) was studied in different chicken and mouse cell lines. the proliferation rate of chicken embryo fibroblasts, either normal or rous sarcoma virus-transformed, was significantly reduced by binase treatment. among mouse fibroblasts, v-ras-transformed nih3t3 cells were sensitive to binase, whereas the growth of non-transformed, v-src-transformed or v-fms-transformed nih3t3 cells was not affect ... | 2001 | 11164612 |
| factors influencing the cellular location of proteolytic enzymes of bacillus intermedius. | thiol-dependent serine proteinase and glutamylendopeptidase of bacillus intermedius 3-19 being prevailing enzymes in the total pool of extracellular proteinases (95%) of this microorganism in catalytic active form were detected on the membrane of the cells. production of these enzymes was maximum on the medium containing inorganic phosphate and gelatin and decreased 2-4-fold on the medium with glucose and lactate. the level of the activity of extracellular enzymes correlated with that of corresp ... | 2000 | 11208276 |
| [medium for the production of bacillus intermedius glutamyl endopeptidase by the recombinant bacillus subtilis]. | a nutrient medium was elaborated for the efficient production of glutamyl endopeptidase by the recombinant bacillus subtilis strain aj73 bearing the bacillus intermedius 3-19 glutamyl endopeptidase gene within a multicopy plasmid. optimal concentrations of the main nutrients, peptone and inorganic phosphate, were found using a multifactor approach. to provide for active growth and efficient glutamyl endopeptidase production, the cultivation medium of the recombinant strain should be enriched in ... | 2000 | 11314652 |
| [localization of proteolytic enzymes in cells of bacillus intermedius]. | the activities of proteinases in the culture fluid and cellular fractions of bacillus intermedius 3-19 grown under various conditions were studied. thiol-dependent serine proteinase was the prevalent enzyme in the total pool of extracellular proteinases (70%); its catalytically active form was also detected in the cell membrane and, during active enzyme production, in the cell wall. another enzyme, glutamyl endopeptidase (10% of the total pool), was detected in the cell membrane; it was also fou ... | 2000 | 11314653 |
| [effect of thermal processing of bacillus intermedius ribonuclease on its catalytic activity and biological properties]. | bacillus intermedius ribonuclease (binase), which is known to exert a growth-stimulating effect at low concentrations and a genotoxic effect at high concentrations, loses these abilities completely after exposure to 100 degrees c for 10 min, but retains approximately 96% of its catalytic activity and structural integrity. other types of modification, such as photoinactivation and site-specific mutagenesis, gave rise to enzyme forms with unaltered structure but reduced (sometimes to trace amounts ... | 2001 | 11338831 |
| enzymatic synthesis of chromogenic substrates for glu,asp-specific proteinases. | glu,asp-specific endopeptidases represent a new subfamily of chymotrypsin-like proteolytic enzymes. these enzymes prefer glu or asp residues in the p1 position of the substrates. p-nitroanilides of n-acylated di-, tri- and tetrapeptides with c-terminal glutamic or aspartic acid residues have been obtained. acyl peptide p-nitroanilides were synthesized via acylation of glutamic or aspartic acid p-nitroanilides using methyl esters of the respective n-acylated peptides, generally with good yields. ... | 2001 | 11454165 |