some properties of a d-alanine carboxypeptidase in envelope fractions of neisseria gonorrhoeae.envelope preparations of neisseria gonorrhoeae strain gc1 (a stable, piliated strain of intermediate colony morphology) and type t1 possess a d-alanine carboxypeptidase which releases the terminal alanine residue from the uridine 5'-diphosphate-n-acetyl muramylpentapeptide substrate (isolated from bacillus cereus t). the d-alanine carboxypeptidase of the gc1 envelopes has a broad ph optimum between ph 8.0 to 10.0. when the molarity of the tris(hydroxymethyl)aminomethane buffer was varied, the ac ...1975332
phospholipase d activity of gram-negative bacteria.a phospholipase hydrolyzing cardiolipin to phosphatidic acid and phosphatidyl glycerol was characterized in gram-negative bacteria but was absent in preparations of gram-positive bacteria, saccharomyces cerevisiae, and rat liver mitochondria. in cell-free extracts of escherichia coli, salmonella typhimurium, proteus vulgaris, and pseudomonase aeruginosa, this cardiolipin-hydrolyzing enzyme had similar ph and mg2+ requirements and displayed a specificity which excluded phosphatidyl glycerol and p ...1975360
regulation of dihydrodipicolinate synthase during growth and sporulation of bacillus cereus.a four- to sixfold increase in specific activity of dihydrodipicolinic acid synthase was observed during sporulation of bacillus cereus. the enzyme from cells harvested before and after the increase in specific activity appeared to be very similar as judged by ph optima, heat denaturation kinetics, apparent michaelis constants, chromatography on diethylaminoethyl-cellulose and sephadex g-200, and polyacrylamide gel electrophoresis. studies with various combinations of amino acids and one of the ...1975367
heat resistance of ileal loop reactive bacillus cereus strains isolated from commercially canned food.sporeformers isolated from a commercially canned food were identified as bacillus cereus, lactose-positive variants. the thermal resistance of spore crops produced from each of two representative cultures was determined in 0.067 m phosphate buffer at ph 7.0. the d121.1 values for one isolate were approximately 0.03 min (z = 9.9c), whereas the d121.1 values for the other isolate were 2.35 min (z = 7.9 c). thermal inactivation results for heat-stressed isolates from each strain showed no significa ...19752108
studies on two isozymes of aconitase from bacillus cereus t. iii. enzymatic properties. 19767257
the occurrence of bacillus cereus in finnish dog food sausages; a microbiological and physiochemical survey.a bacteriological survey of five brands of commercial dog food sausages showed that two of them were heavily contaminated with b. cereus. one of the two brands had a suspected association with food-poisoning incidents in dogs. in addition to b. cereus the other sausage brand was found to contain a high number of sulphite reducing anaerobes. b. cereus was detected in almost every raw material of the two brands of sausages. the pasteurization during manufacturing did not kill all the sporeforming ...19767770
influence of growth temperature on glucose metabolism of a psychotrophic strain of bacillus cereus.the influence of temperature on glucose metabolism of a psychotrophic strain of bacillus cereus was investigated. the ph of the growth medium and spore-forming frequencies of b. cereus varied when grown at 32, 20, or 7 c. radiorespirometric analyses revealed that vegetative cells of b. cereus metabolized glucose by simultaneous operation of the embden-meyerhof-parnas pathway and the pentose phosphate pathway. as the growth temperature decreased, glucose was metabolized with increased participati ...19768003
zn2+ requiring nadp-dependent glutamate dehydrogenase from bacillus cereus t. 19768376
growth of physarum gyrosum on agar plates and in liquid culture.the physical and nutritional requirements of the antibiotic-producing slime mold physarum gyrosum were examined to develop a liquid medium for this myxomycete. liquid culture is desired to expedite a useful scale of production of antibiotic materials for ease of isolation and structure study. culture conditions were selected to favor antibiotic production rather than maximum growth. the medium devised consisted of 0.010 m potassium phosphate buffer (ph 6.0), 2% bakers' yeast, and 0.2% glucose an ...197610830
defined conditions for synthesis of bacillus cereus enterotoxin by fermenter-grown cultures.a strain of bacillus cereus produced high levels of enterotoxin when grown in a semidefined medium in a laboratory scale fermenter. the optimum conditions for enterotoxin synthesis by cultures grown in this medium, which contained casamino acids and yeast extract, were found to be: inoculation of vigorously gorwing culture at the 1% level, addition of glucose at a concentration of 1%, control of culture ph at 8.0, incubation at 32 degrees c, use of a moderate stirring rate, and addition of air a ...197610838
analysis of phospholipase c (bacillus cereus) action toward mixed micelles of phospholipid and surfactant. 197610851
studies on phosphatidylinositol phosphodiesterase (phospholipase c type) of bacillus cereus. i. purification, properties and phosphatase-releasing activity.a phosphatidylinositol phosphodiesterase from the culture broth of bacillus cereus, was purified to a homogeneous state as indicated by polyacrylamide gel electrophoresis, by ammonium sulfate precipitation and chromatography with deae-cellulose and cm-sephadex. the enzyme (molecular weight: 29000 +/- 1000) was maximally active at ph 7.2-7.5, and not influenced by edta, ophenanthroline, monoiodoacetate, p-chloromercuribenzoate or reduced glutathione. the enzyme specifically hydrolyzed phosphatidy ...197610986
validation of a simple radiochemical assay measuring hydrolysis of choline-labelled microsomal phosphatidylcholine by phospholipase c. ph-dependence.selective hydrolysis of phosphatidylcholine species, which are selectively radioactively labelled in vivo, does not appear to interfere with a radiochemical assay for hydrolysis of microsomal phosphatidylcholine by c-type phospholipases from bacillus cereus or clostridium perfringens. both phospholipases substantially hydrolysed phosphatidylcholine over the ph range 4.0-10.0.197613785
germination of unactivated spores of bacillus cereus t. effect of preincubation with l-alanine or inosine on the subsequent germination.heat-activated spores of bacillus cereus t germinate rapidly in the presence of l-alanine alone or inosine alone. in contrast, unactivated spores can not germinate in the presence of either germinant alone but rapidly in the presence of both germinants. the highest level of cooperative action of l-alanine and inosine on the germination was observed when they were present in a ratio 1:1. preincubations of unactivated spores with l-alanine or inosine had opposite effects on the subsequent germinat ...197614271
alterations of spore coat processing and protein turnover in a bacillus cereus mutant with a defective postexponential intracellular protease.a mutant with an alteration in the major intracellular serine protease produced by postexponential bacillus cereus was isolated by screening mutants defective in spore germination. the purified enzyme from the mutant is more labile to heat and alkaline ph than the protease from the wild type. protease activity appears at the same time as in the wild type but only reaches 50% of the specific activity and decays more rapidly during sporulation. coincident with the decay is a decrease in the rate o ...197715254
reversibility of the ampicillin-and nitrite-induced inactivation of beta-lactamase i.beta-lactamase i was isolated from bacillus cereus 569/h. treatment with ampicillin in the presence of sodium nitrite at ph 4 or 5 resulted in the inactivation of the enzyme presumably by modification of a carboxyl group in the active site. however, this inactivation was rapidly, reversible at neutral ph and the available evidence points to the participation of a second carboxyl group which is involved in the reactivation process.197715712
phospholipase c from bacillus cereus. action on some artificial lecithins.the hydrolysis by phospholipase c from b. cereus of several lecithins of different fatty acyl chain length was examined. the enzyme showed significant activity towards mono-molecularly dispersed short chain lecithins and the reaction obeyed normal michaelis-menten kinetics. rate vs. substrate concentration curves obtained with dihexanoyl-, diheptanoyl- and dioctanoyllecithins showed marked discontinuities in the region of the known critical micelle concentrations for these substrates and distinc ...197716420
phospholipase of bacillus cereus.phospholipase activity of 10 strains of bacillus cereus was studied. the most active strain of bac. cereus--phospholipase producer was selected. a cultivation mixture of bac. cereus optimal for the phospholipase synthesis was found to include peptone, yeast extract, glucose, nacl and na2hpo4. proper conditions for the synthesis of phospholipase in flasks, 20 l and 250 l fermenters were tested. the maximum increase of the phospholipase activity occurred by the 5-9th hour of microbial growth at ph ...197617109
effect of cadmium on fungi and on interactions between fungi and bacteria in soil: influence of clay minerals and ph.fungi (rhizopus stolonifer, trichoderma viride, fusarium oxysporum f. sp. conglutinans, cunninghamella echinulata, and several species of aspergillus and penicillium) tolerated higher concentrations of cadmium (cd) when grown in soil than when grown on laboratory media, indicating that soil mitigated the toxic effects of cd. in soil amended with clay minerals, montmorillonite provided partial or total protection against fungistatic effects of cd, whereas additions of kaolinite provided little or ...197718085
studies on the thiamine transport system in bacillus cereus (author's transl).the thiamine transport system in bacillus cereus exhibits rhythmical changes of resorption- and excretion-phases lasting 1-2 h. these main phases are subdivided in shorter ones with an average duration of 45 s. the velocity of the thiamine uptake is influenced by ph, temperature, age of cells, energy and substrate supply and thiamine concentration of the medium. the michaelis-menten-kinetic can be used to describe the uptake: km = 1.98 x 10(-8) m; vmax = 1.19 x 10(-6) mol/g dry weight x min. the ...197719001
purification of phospholipase c from bacillus cereus by chromatography on aminoalkylpolysaccharide adsorbents.purification of phospholipase c from bac. cereus by chromatography on aminoalkylpolysaccharide adsorbents is described. the dependence of the degree of enzyme purification on the amount of ligant and effect of ph and buffer systems on the adsorption-desorption of phospholipase have been studied. at a ph below 9.0 phospholipase c is not retained by the adsorbents and is purified 4-5-fold and up to 23-fold, when aminoalkyl-sepharose and hexamethylenediamine sephadex are used respectively. with an ...197719100
an invitro system for the biosynthesis of spore cortex peptidoglycan. 197719145
dihydrodipicolinate reductases from bacillus cereus and bacillus megaterium.dyhydrodipicolinate reductases were purified 100-fold from crude extracts of b. cereus and b. megaterium and their properties were compared with those of the reductase from b. subtilis. the molecular weights of the reductases of b. cereus and b. megaterium were fount to be 155,000 and 150,000, respectively. these reductases were shown to be free of flavin, unlike the b. subtilis enzyme, which contains flavin. both nadph and nadh acted as coenzymes for these two reductases. nadph being three or f ...197719431
two omega-amino acid transaminases from bacillus cereus.bacillus cereus strain k-22 produced two distinct omega-amino acid transaminases, one catalyzing the transamination between beta-alanine and pyruvic acid and the other that between gamma-aminobutyric acid and alpha-ketoglutaric aic. the two enzymes were partially purified and separated from each other by various chromatographies. beta-alanine:pyruvic acid transaminase and gamma-aminobutyric acid:alpha-ketoglutaric acid transaminase were induced by the addition of beta-alanine and gamma-aminobuty ...197719432
spectrophotometric determination of ribose-1-phosphate. 197720811
n-(2-furyl) acryloyl penicillin: a novel compound for the spectrophotometric assay of beta-lactamase i. 197722529
studies on sphingomyelinase of bacillus cereus. i. purification and properties.a sphingomyelinase was purified 980-fold with recovery of 25.6% from the culture broth of bacillus cereus, by (nh4)2so4 precipitation and chromatography on cm-sephadex, deae-cellulose and sephadex g-75. the purified preparation was free of lipase, protease and other phospholipases. the enzyme specifically hydrolyzed sphingomyelin to ceramide and phosphorylcholine. lysophosphatidylcholine was also attacked by the enzyme. the enzyme (mr = 24 000) was maximally active at ph 6-7. other properties of ...197823854
effect of ph on the antimicrobial activity of some triphenylmethane dyes.four common dyes were tested as inhibitors of four types of bacteria over the ph range 5.0-9.0. inhibition of the gram-negative types, salmonella anatum and enterobacter aerogenes, was markedly affected by the ph of the medium. these organisms tolerated concentrations of crystal violet and ethyl violet about 100-fold higher at ph 5.0 than at ph 9.0. above ph 7.0 brilliant green (bg) and malachite green (mg) were precipitated as their respective carbinols and lost their inhibitory properties with ...197827297
interactions between macromolecular adjuvants and drugs. 11. the influence of macromolecules on the microbiologic activity of tetracycline antibiotics. 197827822
tissue sterility in uneviscerated carcasses.sheep muscle tissue removed aseptically from control carcasses, from uneviscerated carcasses held at 20 degrees c for 24 h, and from carcasses of sheep subjected to stress before slaughter was examined for the presence of bacteria. all samples from a total of 68 carcasses were sterile. whole-body autoradiography of mouse carcasses showed that 14c-labeled fixed bacteria injected after death remained in the lumen of the intestine. live bacteria did not penetrate the mucosal surface until the tissu ...197829564
the influence of high concentrations of carbon dioxide on the germination of bacterial spores. 197831348
effects of glucose, ph, and dissolved-oxygen tension on bacillus cereus growth and permeability factor production in batch culture.the production of a bacillus cereus enterotoxin, measured as rabbit skin permeability factor (pf), in response to differences in glucose availability, ph, and dissolved oxygen tension was studied in a 1-liter batch fermentor system. glucose had to be present for toxigenesis to occur. in uncontrolled fermentation an increasing inhibition of pf production and growth occurred as ph dropped occurred below 6.5. optimum ph for toxigenesis was 7.0 to 7.5, and fermentations maintained at this level yiel ...197932838
histidine residues of zinc ligands in beta-lactamase ii.1. the zn(ii)-requiring beta-lactamase from bacillus cereus 569/h/9, which has two zinc-binding sites, was examined by 270 mhz 1h n.m.r. spectroscopy. resonances were assigned to five histidine residues. 2. resonances attributed to three of the histidine residues in the apoenzyme shift on the addition of one equivalent of zn(ii). 3. although these three histidine residues are free to titrate in the apoenzyme, none of them titrates over the ph range 6.0--9.0 in the mono-zinc enzyme. 4. the abilit ...197833655
production of l-glutamic acid by a bacillus sp.a strain of bacillus cereus var. mycoides isolated from burdwan soil produces l-glutamate in the medium. the strain is able to grow and produce in a synthetic medium but supplementation with casamino acid or yeast extract improves the yield. maintenance of ph of the fermentation medium near neutrality prolongs the active growth period and improves the yield. glucose and ammonium nitrate were found to be most suitable carbon and nitrogen sources, respectively. cane sugar molasses (as a substitute ...197833882
effect of purified phospholipases on the binding of tetrodotoxin to axon plasma membrane.the role of phospholipids in the binding of [3h]tetrodotoxin to garfish olfactory nerve axon plasma membrane was studied by the use of purified phospholipases. treatment of the membranes with low concentrations of either phospholipase a2 (crotalus adamanteus and naja naja) or phospholipase c (bacillus cereus and clostridium perfringens) resulted in a marked reduction in tetrodotoxin binding activity. a 90% reduction in the activity occurred with about 45% hydrolysis of membrane phospholipids by ...197939172
effect of trichloroacetic acid treatment on certain properties of spores of bacillus cereus t.spores of bacillus cereus t treated with trichloroacetic acid (6.1--61.2 mm) were compared with untreated spores, and as the concentration of the chemical increased, the following alterations in spore properties were found: (1) the extent of germination decreased irrespective of the germination medium used; (2) the spores became sensitive to sodium hydroxide (1 n) and hydrochloric acid (0.27 n), but not to lysozyme (200 micrograms/ml); (3) loss of dipicolinate increased on subsequent heating; an ...197941162
an adenosine triphosphate dependent deoxyribonuclease with adenosine triphosphatase, activity from bacillus adenosine triphosphate-stimulated deoxyribonuclease was purified to about 4200 fold from bacillus cereus. the enzyme activity of the crude extract increased by a factor of about 5 after dialysis. one of the low molecular weight inhibitors of the crude extract was found to be inorganic phosphate. during enzyme purification two nucleases were identified. one of them was specific to denatured dna and the other one degraded both denatured dna and native dna. the activity towards native dna could ...197942271
protein synthesizing systems from spores and vegetative cells of bacillus cereus.a system of polyphenylalanine synthesis was optimized for a comparison of the polymerizing activities of ribosomes from spores and vegetative cells of bacillus cereus t. ribosomes of both types react similarly, showing a magnesium optimum of about 6mm and spermidine optima of about 5mm and 4mm for vegative and spore ribosomes, respectively. these lead to optimum mono- to multivalent cation rations of 9 and 10 respectively at 100 mm ammonium ion. a comparison of the response of these ribosomes to ...197942369
[studies of the purification of the exotoxin of bacillus cereus (author's transl)].the exotoxins of bacillus cereus show haemolytic, lethal, phospholipase-c- and enterotoxigenic activities. the enterotoxigenic activity is regarded to be the factor which causes food poisoning in man. efforts to purify the b. cereus exotoxins by precipitation with ammonium sulphate and subsequent chromatography on sephadex-g-75 and biogel-p-60 columns were partially successfull. haemolysin and phospholipase-c could be separated by gel-chromatography, they demonstrated partial identity on gel-dif ...197944788
affinity of cellular constituents of two bacteria for fluorescent brighteners.two fluorescent brighteners were used to stain an isolate of bacillus cereus var. mycoides and soil pseudomonad. the stained organisms were fractionated by two procedures to determine which cellular constituents were reacting with the brighteners. both fractionation procedures provided evidence that the brighteners were adsorbed to proteins within the cells. microscopy examination of ghost cells of the bacillus showed that cell walls were not being stained. spheroplasts of the bacillus and t ...197546739
[bacteriological diagnosis of food poisoning caused by bacillus cereus]. 197548597
the location of bacterial antigens on sections of bacillus cereus by use of the soluble peroxidase--anti-peroxidase complex and unlabelled antibody.the location of antigens on sections of bacteria using the soluble peroxidase-anti-peroxidase complex in conjunction with unlabelled antibody is described. using this technique, spore antigens have been detected in the cytoplasm of vegetative cells during forespore septum formation and subsequent stages of sporulation. antigenic sites were first associated with poly-beta-hydroxybutyric acid granules and subsequently were found in increasing quantities in the cytoplasm of the sporangium. vegetati ...197550408
photobiological behaviour of bacteria and phages supplemented with aza-analogoues of nucleic acid bases.the photochemical stability of anomalous nucleic acid bases of the azatype, 5-azacytosine (i), 5-azacytidine (ii), 6-azacytosine (iii), 6-azacytidine (iv), 6-azathymine (v), 6-azauracil (vi), and 8-aza-adenine (vii) to u. v. light of the wavelength 254 nm differs from the u. v. stability of the normal constituents. changes of the u.v. inactivation of escherichia coli k12 c600, e. coli b, bacillus cereus, as well as e. coli phages gamma cb2 and gamma b2b5 supplemented with azaderivatives prior to ...197552947
effects of phenethyl alcohol on bacillus and streptococcus.the activity of phenethyl alcohol (pea) on bacillus cereus, b. megaterium, and streptococcus faecalis was studied by electron microscopy of thin sections and by the assay of intracellular k+ leakage. s. faecalis was unaffected by pea at concentrations up to 0.5%, b. cereus was severely damaged by 0.5% pea, and b. megaterium behaved intermediately. important membrane ultrastructural alterations were observed in b. cereus cells treated with 0.5% pea, namely the change in the geometry of the membra ...197660333
rational choice of penicillins and cephalosporins based on parallel in-vitro and in-vivo tests.because of the unavailability of strictly comparable data, seven representative penicillins and the five cephalosporins currently used in britain were evaluated in parallel, both in vitro and in vivo. penicillin sensitive and resistant strains of staphylococcus aureus and proteus mirabilis were the main test organisms. minimum bacteriocidal concentrations of cloxacillin, flucloxacillin, cephalothin, and cephazolin in serum were much higher than conventional minimum inhibitory concentrations in t ...197661353
an unusual outbreak of food-poisoning associated with outbreak of food-poisoning after a meals-on-wheels lunch affected 49 persons, 1 of whom died. bacillus cereus serotype 10 and bacillus licheniformis were isolated in large numbers from many of the patients including the deceased and from the remains of the meal. clostridium perfringens (c. welchii) serotype 68, which was isolated from many of the patients and the deceased but not from the food, may also have been responsible. food kept warm during distribution can produce an ideal environment ...197769207
some characteristics of phospholipase c from bacillus cereus. 197772664
[serological properties of species of the genus bacillus]. 197882901
specific staining of bacilli by means of immuno-indian-ink method and immunofluorescent method.the possibilities for identification of b. anthracis are investigated by means of the immuno-indian-ink method (iiim) and the immunofluorescent method (ifm) in their direct and indirect modifications. the specifity of sera against noncapsulated cells of b. anthrasis increases by their adsorption with antigenically related strains of b. cereus as their vegetative cells and spores are killed beforehand with performic acid. repeated uses of the bacillus suspension for adsorption is possible by trea ...197987066
shielding of phospholipid vesicles from phospholipase c hydrolysis by alpha-lactalbumin adsorption [proceedings]. 197992261
binding of exogenous dna by human lymphocytes and by their isolated plasma membranes.the binding of labeled bacterial dna to human tonsil lymphocytes and to plasma membranes isolated from these cells involved macromolecules mainly located at the cell surface, since plasma membrane preparations showed properties in common with those of viable cells. dna binding to lymphocytes was a dissociable, saturable, time, temperature, ph and concentration dependent process. a double reciprocal plot of the data obtained from dna saturation curves gave an apparent dissociation constant of abo ...197994969
hybridization analysis of restriction endonuclease dna fragments of bacillus cereus transcribed during spore outgrowth.transcribing bacillus cereus dna was visualized by means of autoradiography of electrophoretically separated ecori restriction endonuclease dna fragments hybridizing 32p-labeled rna. hybridization of rna of dormant spores, vegetative cells, and outgrowing spores indicates the following. (i) a large fraction of the nonribosomal rna in dormant spores is transcribed at a limited number of regions on the bacterial chromosome. (ii) after induction of spore germination, transcription activity is not l ...197896096
properties of bacillus cereus temperature-sensitive mutants altered in spore coat formation.three conditional bacillus cereus mutants altered in the assembly or formation of spore coat layers were analyzed. they all grew as well as the wild type in an enriched or minimal medium but produced lysozyme and octanol-sensitive spores at the nonpermissive temperature (35 to 38 degrees c). the spores also germinated slowly when produced at 35 degrees c. temperature-shift experiments indicated that the defective protein or regulatory signal is expressed at the time of formation of the outer spo ...197896097
[repeated microcycle in bacillus cereus]. 197896318
the effect of nalidixic acid, rifampicin and chloramphenicol on the synthesis of phospholipase c in bacillus cereus.the effect of nalidixic acid, rifampicin and chloramphenicol on the synthesis of phospholipase c (ec has been studied in washed bacillus cereus cells resuspended in nutrient broth. in the absence of inhibitors, the synthesis showed a biphasic pattern. no synthesis of release of enzyme was found in the presence of chloramphenicol. when rifampicin was added, phospholipase c synthesis continued for 10-15 min. nalidixic acid, at concentrations which inhibited dna synthesis completely, permi ...197896661
bacillus cereus-induced malabsorption in young mice.following a single, oral dose of bacillus cereus (2 x 10(8) bacteria) in vitro intestinal absorption of d-glucose, d-galactose, l-arginine, l-histidine, l-ornithine and l-proline in young mice (aged 2--3 1/2 months) decreased. malabsorption of d-glucose was dose- and time-dependent. impaired absorption of d-glucose occurred throughtout the length of the small intestine, particularly distally. following hydrolysis of d-maltose at the brush border, d-glucose absorption in infected mice and that of ...197897113
an improved electrophoretic method for identifying antibiotics with special reference to animal tissues and animal feeding stuffs. 197897257
preliminary x-ray crystallographic data on phospholipase c from bacillus cereus. 197897390
inhibition by ammonium ion of germination of unactivated spores of bacillus cereus t induced by l-alanine and inosine.studies were carried out on the inhibitory effect of nh4+ on germination of spores of bacillus cereus t induced by l-alanine and inosine. kinetic analysis showed that nh4+ inhibited the germination competitively. its inhibitory effect was greater when the unactivated spores had been preincubated with l-alanine. nh4+ did not inhibit the response of unactivated spores to l-alanine during preincubation. these results suggest that l-alanine sensitizes the spores to the inhibitory effect of nh4+.197897499
bacillus cereus endocarditis. a case report.bacillus cereus may cause infective problems in compromised patients. no previous record of infective endocarditis due to this organism could be found. a 51-year-old white woman with b. cereus endocarditis after prosthetic mitral valve replacement is described. the problems of interpreting the significance of b. cereus bacteraemia, delayed diagnosis, and the inherent resistance of the organism are discussed.197897794
[study of lysogeny in bacillus thuringiensis and b. cereus].forty-eight strains of bacillus thuringiensis and 12 strains of b. cereus were treated with ultraviolet light and mitomycin c. the former agent was the more effective inducer. bacillus thuringiensis produces at least seven different phage particles with long, non-contractile tails. the frequencies of lysogeny and polylysogeny are 83 and 25% respectively. morphologically defective phages occur in 25% of strains, whereas five of them produce low molecular-weight bacteriocins. one strain of b. cere ...197898223
effects of phenylglyoxal on growth & sporulation of bacillus cereus t. 197898431
formation and function of n-acetyloglucosamine-linked phosphoryl- and pyrophosphorylundecaprenols in membranes from bacillus cereus.membranes from bacillus cereus ahu 1356 incorporated radioactivity from udp-n-acetyl[14c]glucosamine into three alkaline-stable and acid-labile lipids which were extracted into chloroform:methanol (2:1) and separated from each other by thin layer chromatography on silica gel plates. the major labeled lipid (lipid 1) and a minor one (lipid 2) were identified as n-actetylglucosaminyl phosphorylundecaprenol from several analytical criteria involving mass spectral data and from reversal of their for ...197898528
two purine nucleoside phosphorylases in bacillus subtilis. purification and some properties of the adenosine-specific phosphorylase.two purine nucleoside phosphorylases (purine-nucleoside:orthophosphate ribosyltransferase, ec were purified from vegetative bacillus subtilis cells. one enzyme, inosine-guanosine phosphorylase, showed great similarity to the homologous enzyme of bacillus cereus. it appeared to be a tetramer of molecular weight 95 000. the other enzyme, adenosine phosphorylase, was specific for adenosine and deoxyadenosine. the molecular weight of the native enzyme was 153 000 +/- 10% and the molecular w ...197899174
[some ultrastructural characteristics of the sporal envelops of bacillus cereus]. 197799733
[microbiological analysis of fruit-flavored toothpastes for children]. 197899797
bacillus cereus as a pathogen. 197899823
understanding the actions of carcinostatic drugs to improve chemotherapy: 5-fluorouracil. 197799991
[method of determining the antimicrobial activity of alcohol extracts of propolis].it is proposed to determine the antimicrobial activity of propolys alcohol extracts by the method of subsequent dilutions in solid nutrient media. dilution of the extracts immediately in hot agar eliminated the inhibiting effect of the extragent on the microbial growth. opalesence appearing in the agar did not prevent estimation of the results in contrast to the method of subsequent dilutions in liquid nutrient media.1978100047
delay in growth induced by glass filtered solar radiation in bacillus cereus. 1978100420
utilization of exogenous purine compounds in bacillus cereus. translocation of the ribose moiety of inosine.intact cells of bacillus cereus catalyze the breakdown of exogenous amp to hypoxanthine and ribose 1-phosphate through the successive action of 5'-nucleotidase, adenosine deaminase, and inosine phosphorylase. inosine hydrolase was not detectable, even in crude extracts. inosine phosphorylase causes a "translocation" of the ribose moiety (as ribose 1-phosphate) inside the cell, while hypoxanthine remains external. even though the equilibrium of the phosphorolytic reaction favors nucleoside synthe ...1978100497
effect of hydrostatic tensile stress on the growth of escherichia coli and bacillus cereus.the specific growth rates of escherichia coli and bacillus cereus were measured for growth media in a flask, a lens-plate arrangement simulating an isolated capillary space, and a lens-plate arrangement under hydrostatic tensile stress. the specific growth rates of the bacteria were the same for the flask and lens-plate arrangement without hydrostatic tensile stress, but were enhanced when the growth media were subjected to hydrostatic tensile stress. the enhanced specific growth rates reached s ...1978101141
studies on phosphatidylinositol phosphodiesterase (phospholipase c type) of bacillus cereus. ii. in vivo and immunochemical studies of phosphatase-releasing activity. 1978101146
isolation of stable ribosomal subunits from spores of bacillus cereus.analyses of ribosomes extracted from spores of bacillus cereus t by a dryspore disruption technique indicated that previously reported defects in ribosomes from spores may arise during the ribosome extraction process. the population of ribosomes from spores is shown to cotain a variable quantity of free 50s subunits which are unstable, giving rise to slowly sedimenting particles in low-mg2+ sucrose gradients and showing extremely low activity in in vitro protein synthesis. the majority of the ri ...1978101512
anti-riboflavin activity of 8n-alkyl analogues of roseoflavin in some gram-positive bacteria.three new 8n-alkyl analogues of roseoflavin (mm), i.e., 8-ethylamino- (eh), 8-methylethylamino- (me), 8-diethylamino-8-demethyl-d-riboflavin (ee), their tetraacetates, and 8-amino-8-demethyl-d-riboflavin (hh) tetraacetate, were synthesized. a relation between the anti-riboflavin activity and the chemical structure of 8n-alkyl analogues (8n-methyl, ethyl) was studied by a restoration by riboflavin (rf) of inhibitory effect of the analogues on a growth of gram-positive bacteria, i.e., sarcina lute ...1978101644
endogenous endophthalmitis associated with bacillus cereus bacteremia in a cocaine addict.a 22-year-old black female intravenous cocaine addict presented with an endophthalmitis of the right eye. diagnostic evaluation included an immediate anterior chamber paracentesis and a delayed vitreous aspiration. although cultures from the involved eye were negative, all 7 blood cultures grew bacillus cereus suggesting that this organism was the responsible agent of an endogenous endophthalmitis. the patient was treated with appropriate systemic and local antibiotics with resolution of the acu ...1978102235
role of cholesterol in the action of cereolysin on membranes. 1978102256
semi-automated detection of emesis in the rhesus monkey.the use of a video recorder and the inclusion of a marker dye in material fed to rhesus monkeys has eliminated the need for the constant presence of an observer to detect an emetic response.1978102332
accessibility of phospholipids in the chromaffin granule membrane.1. the accessibility of phospholipids in the membrane of the adrenomedullary storage vesicles (chromaffin granules) has been studied. 2. the reaction of 2,4,6-trinitrobenzenesulphonic acid with both intact granules and their ghosts, results in the labelling of 70% of the phosphatidylethanolamine. 3. the action of phospholipase a2 (from bee venom), phospholipase c (from bacillus cereus) and sphingomyelinase c (from staphylococcus aureus) on granules and their ghosts was followed as a function of ...1978102348
clavulanic acid inhibition of beta-lactamase i from bacillus cereus 569/h.inactivation of beta-lactamase i by clavulanic acid was investigated. clavulanic acid induced inhibition of the enzyme was found to be progressive with time. benzylpenicillin provided protection against the adverse effects of the inhibitor initially, however, the enzyme was irreversibly inhibited in a progressive manner even in the presence of substrate. reaction of beta-lactamase i with clavulanic acid, in the presence of ampicillin, led to a very rapid inactivation of the enzyme.1978102630
inhibition by potassium ion of the pregerminative response to l-alanine of unactivated spores of bacillus cereus t.the effect of potassium ion on l-alanine-inosine-induced germination of unactivated spores of bacillus cereus t was studied. unactivated spores germinated in 0.1 m sodium phosphate buffer (napb), but not 0.1 m potassium phosphate buffer (kpb), at ph 8.0 and at 30 c. inhibition of germination was also observed on incubation of unactivated spores in napb containing potassium chloride. previously it was demonstrated that germination of unactivated spores involves at least two steps, one induced by ...1978102904
[supplementary approach to the differentiation of the spore bacteria, bacillus subtilis and bacillus cereus]. 1978102905
case report: bacillus cereus food poisoning. 1978103816
relationship of bacillus subtilis dna polymerase iii to bacteriophage pbs2-induced dna polymerase and to the replication of uracil-containing vivo studies of pbs2 phage replication in a temperature-sensitive bacillus subtilis dna polymerase iii (pol iii) mutant and a temperature-resistant revertant of this mutant have suggested the possible involvement of pol iii in pbs2 dna synthesis. previous results with 6-(p-hydroxyphenylazo)-uracil (hpura), a specific inhibitor of pol iii and dna replication in uninfected cells, suggest that pol iii is not involved in phage dna replication, due to its resistance to this drug. experiments were ...1978104052
[enteropathogenicity of bacillus cereus strains]. 1978104365
properties and production characteristics of vomiting, diarrheal, and necrotizing toxins of bacillus cereus.evidence is provided that the enterotoxin of bacillus cereus variously described in the literature as diarrheagenic toxin, diarrheal agent, fluid accumulation factor, vascular permeability factor, dermonecrotic toxin, and intestinonecrotic toxin is a single relatively unstable protein of molecular weight approximately 50,000 and isoelectric point of the order of 4.9. it is presumed to be the enterotoxin responsible for the diarrheal-type b. cereus food poisoning syndrome and it may also be the p ...1979104614
a novel glycosidase, an endo-glucosaminidase active on the cell wall peptidoglycan with n-unsubstituted glucosamine residues. 1979104886
effects of local anesthetics on bacterial cells.the membrane effects of chlorpromazine, nupercain, tetracain, and procain were studied using bacillus cereus, b. megaterium, b. subtilis, and streptococcus faecalis, protoplasts from s. faecalis, and isolated membranes from b. subtilis. chlorpromazin, nupercain, and tetracain produced characteristic micromorphological alterations after treatment for 5 to 30 min at ph 7.0 and 20 degrees c; the membrane staining pattern changed from asymmetric to symmetric, complex mesosome-like structures appeare ...1979104970
bacillus cereus endocarditis. 1979105070
serious infections from bacillus sp.serious infections caused by organisms of the genus bacillus developed in seven patients. five drug abusers had either endocarditis or osteomyelitis, one leukemic patient had necrotizing fasciitis, and one patient had a ventriculoatrial shunt infection with recurrent bacteremia. all patients recovered. experience with these cases reemphasizes the importance of not dismissing bacillus organisms as culture contaminants, especially when isolated from blood, body fluids, or closed-space infections.1979105158
the isolation and cultivation of a single spore using a sterile disposable petri dish and a micromanipulator. 1978105218
deoxyribonucleic acid relatedness between bacillus anthracis, bacillus cereus and bacillus thuringiensis. 1978105229
[information on phase contrast and differential interference contrast figures--a comparative study (author's transl)]. 1978105312
changes in ultraviolet resistance and photoproduct formation as early events in spore germination of bacillus cereus t. 1978105364
[b. cereus count in meat and dairy food products].studies were carried out to establish the contamination of some meat and dairy food products with b. cereus. a total of 48 heat-treated sausages (32 perishable and 16 durable) and 64 batches of pasteurized milk were sampled. it was found that 25 per cent of investigated sausage samples contained b. cereus. perishables proved to a considerable extent more frequently contaminated (33.3 per cent). the count of b. cereus in such products ranged from 10(1) to 10(3) per g. however, pasteurized milk wa ...1978105462
bacillus cereus endogenous panophthalmitis.a case of severe suppurative endogenous panophthalmitis caused by bacillus cereus resulted from intravenously administered medications. this is the first, to our knowledge, well-documented case of endogenous endophthalmitis associated with this organism. it is recommended that if on gram's stain of the anterior chamber fluid, gram-positive rods are seen, chloramphenicol should be administered in addition to penicillin because of the possibility of b cereus infection.1979105693
the inhibition of clostridium chauvoei by bacillus cereus metabolites. 1978105701
conformational studies on phospholipase c from bacillus cereus. the effect of urea on the enzyme.1. when heated in 8 m-urea, phospholipase c(ec from bacillus cereus undergoes conformational transitions depending on the temperatures used. these transitions were studied by examining protein fluorescence, iodide quenching of protein fluorescence, u.v. difference spectroscopy, chemical availability of histidine residues in the enzyme, circular dichroism and catalytic activity. 2. unless simultaneously exposed to elevated temperatures the enzyme appears to be unaffected by 8 m-urea. rem ...1978105729
radiosensitivity of bacteriophages of aerobic spore-forming microorganisms.under conditions of the direct action of radiation on seven original phages of aerobic spore-forming bacteria and four standard phages of e. coli, it was established that they possessed different sensitivity to gamma radiation and were distributed into four groups. the phages of e. coli of the t series, especially t2 phage, proved the most sensitive. the phages of aerobic spore-forming bacilli were characterized by greater resistance to the action of gamma radiation, which is evidently associate ...1978105763
production and characterization of two hemolysins of bacillus cereus.bacillus cereus strain b-48 produced two hemolysins with molecular weights of 52,000 (h-i) and 31,000 (h-ii). a mutant was isolated that produced only h-ii but was identical with the wild type in all other respects. we exploited this mutant to produce h-ii for study that was free of contamination by h-i. by manipulation of media composition, we produced h-i in the absence of h-ii. the hemolysins were precipitated differently by ammonium sulfate, and both exhibited the arrhenius effect when heate ...1978105792
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