| comparative sequence analyses of the 16s rrna genes of lactobacillus minutus, lactobacillus rimae and streptococcus parvulus: proposal for the creation of a new genus atopobium. | 16s rrna gene sequencing was performed on the species lactobacillus minutus, lactobacillus rimae and streptococcus parvulus in order to clarify their taxonomic position. based on comparative sequence analyses these organisms represent a hitherto unknown line of descent within the lactic acid group of bacteria for which a new genus, atopobium gen. nov., is proposed. | 1992 | 1382033 |
| lactobacillus uli sp. nov. and lactobacillus rimae sp. nov. from the human gingival crevice and emended descriptions of lactobacillus minutus and streptococcus parvulus. | lactobacillus uli sp. nov. and lactobacillus rimae sp. nov. are described. these organisms are short, gram-positive, strictly anaerobic, rod-shaped bacteria that have dna g+c contents of 53 and 45 mol%, respectively, produce major amounts of lactic acid, and have been isolated from human gingival crevices and periodontal pockets. the major cellular fatty acid derivatives for both species are c18:1 cis-9 fatty acid methyl ester and c18:1 cis-9 dimethylacetyl. the type strain of l. uli is strain v ... | 1991 | 1854640 |
| recent taxonomic changes for anaerobic gram-positive and selected gram-negative organisms. | the strains formerly classified as streptococcus anginosus or "streptococcus milleri" have now been recognized as three distinct species, streptococcus anginosus, streptococcus constellatus, and streptococcus intermedius. streptococcus morbillorum has been transferred into the genus gemella. four new species within the genus peptostreptococcus were recently named. a recent addition to the genus clostridium is clostridium argentinense, which includes clostridium botulinum type g. two new species ... | 1993 | 8324113 |
| characterization of novel human oral isolates and cloned 16s rdna sequences that fall in the family coriobacteriaceae: description of olsenella gen. nov., reclassification of lactobacillus uli as olsenella uli comb. nov. and description of olsenella profusa sp. nov. | the diversity of organisms present in the subgingival pockets of patients with periodontitis and acute necrotizing ulcerative gingivitis (anug) were examined previously. the 16s rrna genes of subgingival plaque bacteria were amplified using pcr with a universal forward primer and a spirochaete-selective reverse primer. the amplified dna was cloned into escherichia coli. in one subject with anug, 70 clones were sequenced. seventy-five per cent of the clones were spirochaetal, as expected. twelve ... | 2001 | 11594611 |
| new bacterial species associated with chronic periodontitis. | recent investigations of the human subgingival oral flora based on ribosomal 16s cloning and sequencing have shown many of the bacterial species present to be novel species or phylotypes. the purpose of the present investigation was to identify potential periodontal pathogens among these newly identified species and phylotypes. species-specific ribosomal 16s primers for pcr amplification were developed for detection of new species. associations with chronic periodontitis were observed for severa ... | 2003 | 12709498 |
| usefulness of the microseq 500 16s ribosomal dna-based bacterial identification system for identification of clinically significant bacterial isolates with ambiguous biochemical profiles. | due to the inadequate automation in the amplification and sequencing procedures, the use of 16s rrna gene sequence-based methods in clinical microbiology laboratories is largely limited to identification of strains that are difficult to identify by phenotypic methods. in this study, using conventional full-sequence 16s rrna gene sequencing as the "gold standard," we evaluated the usefulness of the microseq 500 16s ribosomal dna (rdna)-based bacterial identification system, which involves amplifi ... | 2003 | 12734240 |
| [a case of bacteremia by atopobium rimae in a patient with liver cirrhosis]. | atopobium rimae, previously lactobacillus rimae, is a strictly anaerobic, non-spore forming grampositive rod which was frequently isolated from odontogenic infection. we report a case of a. rimae bacteremia. a 47-yr-old man with liver cirrhosis was admitted to the hospital via emergency room due to fever and chill. his abdominal and pelvic computed tomography revealed a small abscess near the left adrenal gland. three sets of blood cultures were taken and non-spore forming, grampositive rods wer ... | 2007 | 18094600 |
| human case of atopobium rimae bacteremia. | | 2009 | 19193298 |
| molecular analysis of persistent periradicular lesions and root ends reveals a diverse microbial profile. | the microbial etiology of periradicular lesions is recognized; however, the bacterial profile of these lesions is not well elucidated. the purpose of this study was to determine the frequency of bacterial colonization in these lesions and to characterize the bacterial community present in root ends and periradicular lesions. | 2009 | 19567313 |
| exploring bacterial diversity of endodontic microbiota by cloning and sequencing 16s rrna. | the characterization of microbial communities infecting the endodontic system in each clinical condition may help on the establishment of a correct prognosis and distinct strategies of treatment. the purpose of this study was to determine the bacterial diversity in primary endodontic infections by 16s ribosomal-rna (rrna) sequence analysis. | 2011 | 21689545 |
| photoinactivation using visible light plus water-filtered infrared-a (vis+wira) and chlorine e6 (ce6) eradicates planktonic periodontal pathogens and subgingival biofilms. | alternative treatment methods for pathogens and microbial biofilms are required due to the widespread rise in antibiotic resistance. antimicrobial photodynamic therapy (apdt) has recently gained attention as a novel method to eradicate pathogens. the aim of this study was to evaluate the antimicrobial effects of a novel apdt method using visible light (vis) and water infiltrated infrared a (wira) in combination with chlorine e6 (ce6) against different periodontal pathogens in planktonic form and ... | 2016 | 27965635 |
| oral microbiota species in acute apical endodontic abscesses. | acute apical abscesses are serious endodontic diseases resulting from pulpal infection with opportunistic oral microorganisms. the objective of this study was to identify and compare the oral microbiota in patients (n=18) exhibiting acute apical abscesses, originating from the demographic region in portland, oregon. the study hypothesis is that abscesses obtained from this demographic region may contain unique microorganisms not identified in specimens from other regions. | 2016 | 26983837 |
| new bacterial composition in primary and persistent/secondary endodontic infections with respect to clinical and radiographic findings. | the aim of the present study was to analyze the microbiota of primary and secondary/persistent endodontic infections of patients undergoing endodontic treatment with respect to clinical and radiographic findings. | 2014 | 24767562 |
| atopobium rimae bacteremia complicated by an infection-related glomerulonephritis in a cardiac transplanted patient. | | 2016 | 27145541 |
| randomized in vivo evaluation of photodynamic antimicrobial chemotherapy on deciduous carious dentin. | the aim of this randomized in vivo study was to compare antimicrobial chemotherapies in primary carious dentin. thirty-two participants ages 5 to 7 years underwent partial caries removal from deep carious dentin lesions in primary molars and were subsequently divided into three groups: control [chlorhexidine and resin-modified glass ionomer cement (rmgic)], ledtb [photodynamic antimicrobial chemotherapy (pact) with light-emitting diode associated with toluidine blue solution and rmgic], and lmb ... | 2015 | 26502235 |
| development of quantitative real-time pcr primers for detecting 42 oral bacterial species. | in this study, we introduced species-specific quantitative real-time pcr (qpcr) primers designed based on a dna-dependent rna polymerase beta-subunit gene (rpob) for detecting 42 oral bacterial species. the specificity of the qpcr primers was confirmed by conventional pcr with the genomic dnas of 73-79 strains regarding 73-75 bacterial species including the type strain for the target species. the standard curves revealed the lower detection limits of 42 bacterial species-specific qpcr primers ra ... | 2013 | 23689247 |
| microbial transformation from normal oral microbiota to acute endodontic infections. | endodontic infections are a leading cause of oro-facial pain and tooth loss in western countries, and may lead to severe life-threatening infections. these infections are polymicrobial with high bacterial diversity. understanding the spatial transition of microbiota from normal oral cavities through the infected root canal to the acute periapical abscess can improve our knowledge of the pathogenesis of endodontic infections and lead to more effective treatment. we obtained samples from the oral ... | 2012 | 22839737 |