| serum bactericidal activity in the horseshoe crab, limulus polyphemus. | serum from the horseshoe crab, limulus polyphemus, was examined for bactericidal activity against five species of bacteria. greatest activity was found against pseudomonas putida and flavobacterium sp.; with the former, serum dilutions as high as 1:20 were capable of reducing viable counts by 50% within 2 h. bactericidal activity of a significantly lesser magnitude was demonstrated against serratia marcesencs and salmonella minnesota. no killing was seen when the lobster pathogen aerococcus viri ... | 1976 | 11188 |
| the activities in vitro of dd-carboxypeptidase and ld-carboxypeptidase of gaffkya homari during biosynthesis of peptidoglycan. | | 1978 | 25767 |
| aldolases of the lactic acid bacteria. demonstration of immunological relationships among eight genera of gram positive bacteria using an anti-pediococcal aldolase serum. | reciprocal qualitative and quantitative immunological experiments employing an anti-pediococcus cerevisiae aldolase serum confirmed many of the interspecific relationships demonstrated previously among lactic acid bacteria with antisera prepared against the streptococcus faecalis fructose diphosphate aldolase. the extent of immunological relatedness observed between the lactobacillus and pediococcus aldolases was markedly gses indicating that the pediococci share closer phylogenetic ties with th ... | 1976 | 65160 |
| growth inhibition of escherichia coli w by d-norvalyl-d-alanine: an analogue of d-alanine in position 4 of the peptide subunit of peptidoglycan. | position 4 analogues of d-alanine in the peptide subunit of uridine 5'-diphosphate-n-acetylmuramyl-ala(1)-dglu(2)- lys(3)-dala(4)-dala(5) have a significant inhibitory effect on penicillin-sensitive peptidoglycan synthesis in gaffkya homari (c. v. carpenter, s. goyer, and f. c. neuhaus, 1976). the specificity profile of this in vitro system has been used as a basis for designing analogues with potential antibacterial activity. to circumvent the specificity determinants exerted by d-alanine:d-ala ... | 1977 | 324397 |
| [bacterial flora of the conjunctival sac of the horse]. | the aa. report the results of taxonomic research conducted on the conjunctival sac of 59 horses for identification of the present bacterial flora. in the controlled animals, it was observed, at the level of the considered niche, a community constituted of normal bacterial populations, but not autochtonous in the significance they attributed from dubos et al., relative to the characterization of the indigenous microbiota of the intestine. the isolated normal bacterial flora seems to be constitute ... | 1976 | 788654 |
| fluorescent substrate for nascent peptidoglycan synthesis. uridine diphosphate-n-acetylmuramyl-(nepsilon-5-dimethylaminonaphthalene-1-sulfonyl)pentapeptide. | the synthesis of udp-murnac-ala-dglu-lys[nepsilon-dimethylaminonaphthalene sulfonyl (dns)]-dala-dala provides a method for the specific introduction of a fluorescent reporter group into the membrane environment of nascent peptidoglycan synthesis. to assess the degree of perturbation of this environment caused by the introduction of the dansyl substituent, this nucleotide was compared with udp-murnac-ala-dglu-lys-dala-dala in the reaction catalyzed by phospho-murnac-pentapeptide translocase (udp- ... | 1977 | 849930 |
| serological grouping of virulent and avirulent strains of the lobster pathogen aerococcus viridans. | virulent strains of aerococcus viridans (formerly gaffkya homari) are the aetiologic agents of gaffkemia, a septicaemic disease of the american lobster (homarus americanus). the virulent and avirulent forms of this bacterium, previously thought to be taxonomically indistinguishable, have been differentiated by serological studies. antisera were produced in rabbits using autoclaved bacteria as antigens. reactions were measured by agglutination tests using microtitre techniques and an antigenic sc ... | 1977 | 874455 |
| [viridicine: a bacteriocine from "aerococcus viridans" (author's transl)]. | viridicine, a new bacteriocine, was extracted from whole cells of aerococcus viridans by repeated washings with tris-hcl 5 x 10(-3) m ph 8,1, nacl 1 m. a crude preparation of the macromolecule was not inactived by heating for one hour at 50 degrees c and was sensitive to the action of protease, lipase and catalase. the molecular weight was about 100,000. a large number of gram-negative and gram-positive bacteria were sensitive to viridicine action. | 1977 | 911106 |
| 4-o-[(s)-1-carboxyethyl]-d-glucose: a component of the extracellular polysaccharide material from aerococcus viridans var. homari. | | 1976 | 1009540 |
| endocarditis and osteomyelitis caused by aerococcus viridans. | | 1976 | 1084468 |
| identification of some uncommon monounsaturated fatty acids of bacteria. | location of the double-bond position of monounsaturated fatty acids of various bacteria was accomplished with combined gas chromatography-mass spectrometry analysis of dimethyl disulfide (dmds) derivatives. the monoenoic fatty acids from whole cells were converted to methyl esters and then to dmds adducts and analyzed by capillary gas chromatography-mass spectrometry. the mass spectra of dmds adducts gave an easily recognizable molecular ion and two major diagnostic ions attributable to fragment ... | 1992 | 1401029 |
| rapid identification of aerococcus viridans using the polymerase chain reaction and an oligonucleotide probe. | a polymerase chain reaction/oligonucleotide probe method was developed for the specific identification of the gram-positive bacterium aerococcus viridans. primers for the enzymatic amplification reaction were designed from specific sequences within the 16s rrna. the method was also highly sensitive and 10 cfu of a. viridans could be detected in 5 h although the reliability of detection was poor in mixed cultures with escherichia coli. | 1992 | 1516808 |
| interactions between bacteria and influenza a virus in the development of influenza pneumonia. | different proteases from various microorganisms present in the respiratory tract were capable of enhancing influenza virus infectivity and pathogenicity in mice by proteolytic activation of hemagglutinin (ha). aerococcus viridans, isolated from a patient with pneumonia, secreted a protease that could activate ha directly, similarly to some staphylococcus aureus strains. the protease of pseudomonas aeruginosa could not activate ha directly, but combined application of p. aeruginosa protease and v ... | 1992 | 1527412 |
| biosynthesis of peptidoglycan in gaffkya homari: on the target(s) of benzylpenicillin. | the formation of acceptor for the n epsilon-(d-ala)-acceptor transpeptidase is an essential feature of nascent peptidoglycan processing. in gaffkya homari the synthesis of cross-bridges in peptidoglycan includes a variety of penicillin-sensitive enzymes, e.g., transpeptidase, dd-carboxypeptidase, and ld-carboxypeptidase. to determine the primary target, we grew cultures in the presence of the mics of benzylpenicillin (0.2 microgram/ml), methicillin (10 micrograms/ml), cephalothin (5 micrograms/m ... | 1991 | 1952843 |
| structural studies of the capsular polysaccharide from aerococcus viridans var. homari. | the capsular polysaccharide from aerococcus viridans var. homari has been investigated, using n.m.r. spectroscopy, methylation analysis, and specific degradations as the main methods. the polysaccharide is composed of tetrasaccharide repeating-units having the following structure. (formula; see text) in this structure, d-quin stands for 2-amino-2,6-dideoxy-d-glucose (quinovosamine). two of the three acidic sugars found, namely, l-altruronic acid and 4-o-[(s)-1-carboxyethyl]-d-glucose, have not b ... | 1990 | 2085808 |
| [septic arthritis as an initial manifestation of endocarditis]. | a 60-year-old female patient was admitted to the hospital with high fever and an acute onset of pauciarticular arthritis. clinical examination and ultrasound imaging of the right knee demonstrated a severe synovitis with a large synovial effusion and a baker's cyst. arthrocentesis showed an opaque viscous synovial fluid with a highly elevated white blood cell count. in synovial fluid cultures and in serial blood cultures aerococcus viridans (av) was detected. further examination revealed a mitra ... | 1990 | 2198741 |
| the phylogeny of aerococcus and pediococcus as determined by 16s rrna sequence analysis: description of tetragenococcus gen. nov. | the phylogenetic interrelationships of the genera pediococcus and aerococcus were investigated using reverse transcriptase sequencing of 16s rrna. the genus pediococcus was found to be phylogenetically heterogeneous. the four species p. acidilactici, p. damnosus, p. parvulus and p. pentosaceus formed a phylogenetically distinct group. within this pediococcal cluster, p. acidilactici was closely related to p. pentosaceus whereas p. damnosus showed a specific relationship with p. parvulus. the spe ... | 1990 | 2227360 |
| aerococcus viridans infection. case report and review. | | 1990 | 2242644 |
| phenotypic characterization, cellular fatty acid composition, and dna relatedness of aerococci and comparison to related genera. | aerococci can be misidentified as streptococci, enterococci, pediococci, lactococci, or leuconostocs. to distinguish the genus and determine if another species is needed in the present taxon, we analyzed 37 aerococci for cellular fatty acids and compared them with 377 strains of gram-positive cocci, including the species type strains from each of the related genera. the cellular fatty acid profile of aerococci was distinguishable from other genera. two relatively novel fatty acids found in the a ... | 1990 | 2324269 |
| inhibition of peptidoglycan biosynthesis by ramoplanin. | ramoplanin, a new lipoglycopeptide antibiotic, inhibits cell wall peptidoglycan biosynthesis in gram-positive bacteria. in both staphylococcus aureus and bacillus megaterium, udp-n-acetylmuramyl-pentapeptides (udp-murnac-pentapeptides) accumulated at concentrations of ramoplanin close to the mic, indicating that inhibition of peptidoglycan biosynthesis occurred after formation of cytoplasmic precursors. susceptible bacteria bound or accumulated approximately 5 x 10(4) molecules of ramoplanin per ... | 1990 | 2334153 |
| minimum inhibitory concentrations and disk diffusion zone diameter for selected antibiotics against streptococci isolated from bovine intramammary infections. | the antimicrobial susceptibility to 11 antibiotics was determined of 358 streptococcal isolates representing six species was determined. all isolates were from milk samples obtained from clinical or subclinical cases of bovine mastitis. agar disk diffusion zone diameters and minimum inhibitory concentrations were obtained for all isolates. of the antibiotics tested that are available for mastitis therapy, cephalothin, penicillin, ampicillin, novobiocin, and erythromycin demonstrated acceptable a ... | 1990 | 2365883 |
| [the lactate oxidase activity of aerococcus viridans cultures]. | the oxidase activity of aerococcus viridans is a result of the functioning of aerobic nad-independent lactate dehydrogenase. two fractions of flavin-containing protein which oxidize d- and l-isomers of lactic acid are revealed during the enzymic complex electrophoresis in paag. the enzymic complex and eluates of both fractions possess the antagonistic activity relative to 27 test-cultures of bacteria. | 1989 | 2622398 |
| [the effect of aerococcus viridans--the basis of the new therapeutic-prophylactic preparation m-bacterin--on the biological properties of staphylococcus aureus]. | representatives of the normal microflora from genus aerococcus, in particular strain aerococcus viridans 167 isolated from breast milk are studied for their effect on biological properties of staphylococcus aureus in vitro and in vivo. it is established that the number of viable cells of the staphylococcus cultivated in the presence of antagonists in the beef-extract agar decreases progressively with each following passage, the population dying after the seventh-eight passage. electronograms fix ... | 1989 | 2622400 |
| genetic basis of antibiotic resistance in aerococcus viridans. | resistance to at least one of the following antibiotics was found in eight wild-type strains of aerococcus viridans: erythromycin (six strains), tetracycline and minocycline (five strains), chloramphenicol (one strain), and high levels of streptomycin (one strain). none of the strains transferred any of their antibiotic resistance markers into streptococcal, enterococcal, or a. viridans recipients by conjugation. by dna-dna hybridization experiments, the ermb gene of transposon tn917, of enteroc ... | 1989 | 2729946 |
| aerococcus-like organism--an unnoticed urinary tract pathogen. | aerococci are only occasionally isolated from urinary tract specimens. a 4-month' survey revealed 29 patients suspected of urinary tract infection (uti), from whom an aerococcus-like organism (alo) was isolated in significant numbers from urinary tract specimens and singly in 11 cases. approximately 0.8% of all urinary tract specimens examined during the period yielded growth of alo. the median age of patients was 75 years. half of the patients were found to have conditions predisposing to uti. ... | 1989 | 2736106 |
| intramembranal events in the biosynthesis of peptidoglycan in gaffkya homari. | in order to monitor the intermediates involved in nascent peptidoglycan (pg) assembly in gaffkya homari, a pulse/chase assay utilizing udp-murnac-ala-dglu-lys(n epsilon-dns)-dala-dala [dns (dansyl) = 5-(dimethylamino)naphthalene-1-sulfonyl] was devised. the perturbation introduced by the dansyl group provided a means for separating the synthesis of nascent pg into discrete stages. together with paramagnetic quenching of the fluorophore by n-doxylstearic acids (n = 5, 7, 12, 16; doxyl = n-oxy-4', ... | 1987 | 3103675 |
| [the action of hydrogen peroxide produced by aerococcus viridans on escherichia coli and bacillus subtilis]. | | 1987 | 3150491 |
| teat skin normal flora and colonization with mastitis pathogen inhibitors. | isolates of bacteria from normal teats were used to attempt colonization of teats of dry cows or neonatal calves. isolates for inoculation were chosen on the basis of ability to inhibit mastitis pathogens in vitro, with the ultimate goal of in vivo inhibition of mastitis pathogens at the teat surface. three bacterial normal flora isolates (corynebacterium xerosis, bacillus sp. and aerococcus viridans) persisted less than 10 days on the teats of dry cows. the fourth isolate, staphylococcus homini ... | 1988 | 3188374 |
| ofloxacin for prevention of bacterial infections in granulocytopenic patients. | we studied the potential value of oral ofloxacin (200 mg twice daily) for selective decontamination and infection prevention in 40 granulocytopenic patients with acute leukemia, blast crisis of chronic myelogenous leukemia, hairy cell leukemia or severe aplastic anemia. the quality of selective decontamination was acceptable with rapid elimination of enterobacteriaceae from the alimentary tract, only a slight decrease in concentrations of anaerobes in faeces, and a small number of newly acquired ... | 1987 | 3481358 |
| effects of metals on the chemiluminescent response of rainbow trout (salmo gairdneri) phagocytes. | quantification of an induced chemiluminescent (cl) response in phagocytes is currently being evaluated as an indicator system for determining those environmental pollutants that may predispose fish to disease. a cl assay was developed using phagocytes from the pronephros of rainbow trout (salmo gairdneri). the cl response of phagocytes to phorbol myristate acetate, a chemical inducer of cl, was shown to be dose-dependent. the response to five species of bacteria was also evaluated. staphylococcu ... | 1986 | 3765345 |
| cross-reactions between alpha-streptococci and omniserum, a polyvalent pneumococcal serum, demonstrated by direct immunofluorescence, immunoelectroosmophoresis, and latex agglutination. | in recent years several groups have used serological methods to demonstrate pneumococcal capsular antigens in sputum. in the present study 123 strains of alpha-hemolytic streptococci (including 97 strains from sputum or pharyngeal specimens) were tested for cross-reactions with a polyvalent antipneumococcal serum (omniserum). representatives of the following species were included: streptococcus bovis, s. equinus, s. intermedius, s. lactis, s. milleri, s. mitis, s. mutans, s. sobrinus, s. salivar ... | 1985 | 3889046 |
| [stomatococcus mucilaginosus: cultural and biochemical properties of 100 strains isolated from the orl area]. | a study of the growth characteristics and 80 biochemical tests using api system micromethods (api-50ch, api-20e, api-zym) was carried out on 100 strains of stomatococcus mucilaginosus isolated from the human oral cavity. seventy-seven other enzymatic tests from the api system (ap-arylamidases, osidases and esterases) were also performed on 10 of these strains. the results of these tests were compared to those of 24 type strains of micrococcaceae and aerococcus viridans. a simple identification s ... | 1985 | 3890697 |
| wall peptidoglycan in aerococcus viridans strains 201 evans and atcc 11563 and in gaffkya homari strain atcc 10400. | | 1971 | 4254570 |
| biosynthesis of peptidoglycan in gaffkya homari: role of the peptide subunit of uridine diphosphate-n-acetylmuramyl-pentapeptide. | the incorporation of n-acetylmuramyl (murnac)-peptides from nucleotide-activated precursors (reference: uridine diphosphate [udp]murnac-ala(1)-dglu(2)-lys(3)- dala(4)-dala(5)) with incomplete or modified peptide subunits into peptidoglycan was studied with membrane preparations from gaffkya homari. the effectiveness of their utilization at low and high concentrations was compared on the basis of the values of v(max)/k(m) and v(max), respectively. at low concentration, replacement of alanine by g ... | 1974 | 4425467 |
| on the mechanism of action of vancomycin: inhibition of peptidoglycan synthesis in gaffkya homari. | vancomycin inhibits the synthesis of peptidoglycan in membrane preparations from gaffkya homari with uridine diphosphate-n-acetylmuramyl (udp-mur-nac)-pentapeptide as substrate, but not with either udp-murnac-tetrapeptide or udp-murnac-tripeptide. these results are correlated with the specificity studies described by perkins and nieto for complex formation between the antibiotic and the peptide subunit. it is concluded that the formation of a complex between vancomycin and a postulated cell wall ... | 1974 | 4451345 |
| depletion of glycogen and adenosine triphosphate as major factors in the death of lobsters (homarus americanus) infected with gaffkya homari. | | 1973 | 4754747 |
| deoxyribonucleic acid homology among strains of the lobster pathogen 'gaffkya homari' and aerococcus viridans. | | 1974 | 4822121 |
| effects of gaffkya homari on glucose, total carbohydrates, and lactic acid of the hemolymph of the lobster (homarus americanus). | | 1972 | 5071236 |
| gaffkemia, a bacterial disease of the lobster, homarus americanus: effects of the pathogen, gaffkya homari, on the physiology of the host. | | 1969 | 5344746 |
| nutritional requirements for growth of aerococcus viridans. | | 1970 | 5489060 |
| recognition of aerococcus viridans by the clinical microbiologist. | | 1970 | 5507757 |
| aerococcus viridans in the hospital environment. | aerococcus viridans has been described as an airborne organism prevalent in occupied rooms. it has also been described as an organism having many characteristics that might cause it to be confused with streptococci or staphylococci, and this may account for the fact that the presence of a. viridans has not been reported in the hospital environment or in clinical specimens. swab specimens were taken from 47 objects in 11 different areas in a local hospital, cultured overnight in trypticase soy br ... | 1968 | 5649865 |
| [greening of cooked ham by aerococcus viridans and study of its thermic resistance]. | | 1968 | 5731455 |
| acute meningitis in early childhood caused by aerococcus viridans. | | 1983 | 6404411 |
| biosynthesis of peptidoglycan in gaffkya homari: processing of nascent glycan by reactivated membranes. | membranes from gaffkya homari reactivated by freezing and thawing were used to study the processing events involved in the assembly of both sodium dodecyl sulfate (sds)-insoluble peptidoglycan (pg) and sds-soluble pg. the ability to reactivate membranes for the synthesis of these polymers provided an opportunity to monitor those events that are not influenced by wall-linked pg. in g. homari, processing for the formation of cross-links requires the selective actions of dd-carboxypeptidase, ld-car ... | 1984 | 6693347 |
| aerococcus viridans endocarditis. | aerococcus viridans organisms are gram-positive cocci with a strong tendency to form tetrads. these bacteria have infrequently been encountered as a human pathogen, particularly in bacterial endocarditis. a review of the literature suggests that treatment of a. viridans endocarditis should be similar to that for endocarditis caused by penicillin-susceptible streptococci. | 1984 | 6694434 |
| two epidemics of pseudobacteremia due to staphylococcus aureus and aerococcus viridans. | two epidemics of pseudobacteremia are reported. the first, due to staphyloccus aureus, was caused by a physician who had active staphylococcal skin infection and nasal colonization. because the blood culture system in use at the time was open, and used screw cap bottles, we assume that the physician contaminated the bottles at the time of inoculation. the second outbreak, caused by aerococcus viridans, was traced to contamination of the blood culture bottle tops as they were received from the ma ... | 1980 | 6904394 |
| aerococcus viridans as a causative agent of infectious endocarditis. | identification and properties of aerococcus viridans isolated from the blood of a patient with infectious endocarditis are described. the role of this agent in the pathogenesis of this disease was demonstrated by repeated cultivations as well as by high titres of specific antibodies in the patient's serum. pathogenicity of this strain for experimental animals was not demonstrated. | 1980 | 7190591 |
| purification of the viridicin produced by aerococcus viridans. | a new bacteriocin produced by aerococcus viridans was purified. bacteria grown on liquid medium synthesized a bacteriocin, viridicin, which can be extracted from the cells by treatment with 0.86 m nacl solution. viridicin production was not induced by ultraviolet irradiation or by treatment with mitomycin c. the bacteriocin was purified by ultrafiltration, gel filtration, and preparative polyacrylamide disc gel electrophoresis. the molecular weight was between 100,000 and 120,000. the purified v ... | 1980 | 7396467 |
| the direct application of the polymerase chain reaction to dna extracted from foods. | two methods for the successful extraction of dna from foods are described. the rapid lysis method uses a proteinase k buffer system to lyse cells and solubilize food samples. dna is then precipitated using isopropanol. the second method achieves cell lysis using toluene and mutanolysin, and solubilization using guanidium thiocyanate. following protein removal with organic solvents dna is precipitated with isopropanol. both methods enabled the polymerase chain reaction to be applied directly to d ... | 1995 | 7766115 |
| growth of enterococcus, lactococcus and streptococcus strains and environmental isolates in liquid media and their reactions on beea. | growth of known species of enterococcus, lactococcus and streptococcus and aerococcus viridans in selective and nonselective liquid media routinely used to enumerate faecal streptococci was measured optically at different temperatures. growth of environmental isolates was measured in some of these media. growth of the reference strains on bile esculin azide agar at elevated incubation temperatures was tested. the results revealed only minor differences between media but strong influence of incub ... | 1994 | 7811573 |
| aerococcus viridans endocarditis. case report. | | 1994 | 7875897 |
| [the stimulating effect of aerococcus viridans on phagocytosis and antibody formation]. | | 1994 | 7879500 |
| gram-positive cocci isolated from slaughtered poultry. | gram-positive cocci were found in all meat samples of poultry slaughtered and processed for retail sale, at incidence rates ranging from 10(2) cfu/ml to 1.35 x 10(6) cfu/ml and a mode between 8 x 10(5) and 9 x 10(5) cfu/ml for 75% of the samples. the 93 isolated strains were identified as belonging to the following species: enterococcus faecalis (48 strains), e. faecium (16), e. avium (7), e. durans (4), aerococcus viridans (10), streptococcus morbillorum (2), s. salivarius (1), s. sanguis (1), ... | 1994 | 7921897 |
| in vitro inhibition of some gram-positive bacteria by staphylococci and aerococcus viridans of porcine origin. | nine coagulase-negative staphylococci and aerococcus viridans were tested for bacterial antagonism. seven of the nine of the coagulase-negative staphylococci and aerococcus viridans inhibited the growth of four staph. hyicus, three staph. aureus strains and some other gram-positive bacteria, including streptococcus group a and d, corynebacteria, but none of the gram-negative and yeast was inhibited on agar media. there was also production of inhibitory substances in different types of liquid med ... | 1993 | 8004048 |
| septic arthritis due to aerococcus viridans. | a 20-year-old woman was found to have septic arthritis of the hip due to aerococcus viridans. this organism closely resembles streptococcus viridans, but forms gram positive tetrads rather than chains in broth media. the organism has been reported rarely to cause endocarditis and one case of osteomyelitis has been observed. to our knowledge septic arthritis due to aerococcus viridans has not been previously reported. | 1985 | 4087248 |
| atmospheric growth requirements for alloiococcus species and related gram-positive cocci. | the growth of alloiococcus otitis under different atmospheres and nutritional conditions was studied. the growth rates of 25 strains of gram-positive cocci representing five genera on heart infusion agar plates containing 5% rabbit blood and on brucella agar plates with and without sheep blood under aerobic, increased co2, and anaerobic atmospheres were compared. eight strains of alloiococci plated on heart infusion agar with rabbit blood and on brucella sheep blood agar grew under aerobic and c ... | 1996 | 8815077 |
| penicillin-resistant aerococcus viridans bacteremia in a child receiving prophylaxis for sickle-cell disease. | | 1996 | 8838213 |
| solid-state 15n nmr studies of the effects of penicillin on cell-wall metabolism of aerococcus viridans (gaffkya homari). | lyophilized whole cells and isolated cell walls from aerococcus viridans (gaffkya homari) grown on a synthetic medium containing benzylpenicillin, and either l-[epsilon-15n]lysine or 15n-ammonium ion as the only source of label, have been studied using cross-polarization magic-angle spinning 15n nuclear magnetic resonance. the lysine is incorporated directly into protein and cell-wall peptidoglycan and was used to measure cell-wall cross-links. the ammonium ion acts as a non-specific label monit ... | 1985 | 3977898 |
| inhibition of peptidoglycan biosynthesis in vancomycin-susceptible and -resistant bacteria by a semisynthetic glycopeptide antibiotic. | ly191145 is a p-chlorobenzyl derivative of ly264826 (a82846b) with activity against both vancomycin-susceptible and -resistant enterococci. incorporation of l-[14c]lysine into peptidoglycan of intact vancomycin-susceptible and -resistant enterococcus faecium was inhibited by ly191145 (50% inhibitory concentrations of 1 and 5 microgram/ml, respectively). inhibition was accompanied by accumulation of udp-muramyl-peptide precursors in the cytoplasm. this agent inhibited late-stage steps in peptidog ... | 1996 | 8891144 |
| solid-state 13c and 15n nuclear magnetic resonance studies of alanine metabolism in aerococcus viridans (gaffkya homari). | transport and metabolism of d- and l-alanine by aerococcus viridans (gaffkya homari) have been studied using cross-polarization magic-angle spinning 13c and 15n nmr spectroscopy of lyophilized whole cells, isolated cell walls, and crude extracts. for equimolar concentrations in the growth medium, about 10 times more d-alanine than l-alanine is transported into the cells. examination of cells labeled with d-[13c]alanine and l-[epsilon-15n]lysine by double cross-polarization magic-angle spinning 1 ... | 1985 | 3972803 |
| characterization of peptidoglycan stem lengths by solid-state 13c and 15n nmr. | lyophilized whole cells of aerococcus viridans (gaffkya homari) grown on a synthetic medium containing d-[2-13c, 15n]ala, or containing both l-[1-13c]lys and d-[15n]ala, have been examined by double cross-polarization magic-angle spinning 13c and 15n nuclear magnetic resonance. results from the double-labeled alanine experiment confirm the absence of metabolic scrambling of alanine by a. viridans. results from the combined single-label experiment can be used to count directly the number of adjac ... | 1986 | 3729935 |
| in vivo target of benzylpenicillin in gaffkya homari. | it has been established that the dd-carboxypeptidase is the primary in vitro target of benzylpenicillin in gaffkya homari (w. p. hammes, eur. j. biochem. 70:107-113, 1976). to determine whether this enzyme is also the primary target of benzylpenicillin in vivo, we compared the effects of this beta-lactam, cefmenoxime, cephalothin, and cefoxitin on growth with their acylation of penicillin-binding protein (pbp) 9, the dd-carboxypeptidase. results of three types of experiments with membrane-walls ... | 1986 | 3717943 |
| [a biological model study of the effect of aerococcus viridans on pathogenic bacteria]. | morphological researches studies showed that the treatment of infected skin wounds with aerobact quickly eliminated inflammation, promoted regeneration of damaged tissues, that actively stimulated formation of granulations. a decrease of s. aureus virulence which completely or partially loose lethal and necrotic activity is one of the reasons of the observed phenomenon. on the model of s. typhimurium infection the expressed protective action of bacteria of aerococcus genus was shown; this gives ... | 1998 | 9785800 |
| airways microbial flora in copd patients in stable clinical conditions and during exacerbations: a bronchoscopic investigation. | patients affected with chronic obstructive pulmonary disease (copd) undergo frequent exacerbations of their illness characterized by increased cough and expectoration. the precise aetiology of these episodes often remains unknown. in the absence of clinical or radiographic signs of pneumonia, bacterial or viral cultures of sputum usually provide little useful information. thus, we performed fibreoptic bronchoscopy using a protected specimen brush (psb) to obtain uncontaminated secretions for cul ... | 1998 | 9785808 |
| [indications and results of surgery in native valve infectious endocarditis. apropos of 104 surgically-treated cases]. | from 1972 to 1984, 104 cases of aortic valve infectious endocarditis were treated surgically. the average age of the patients was 40 years and the majority were men (69/104). forty patients had no previous cardiac disease; 44 patients had documented valvular heart disease, which was unlikely in the remaining 20 patients. there were 16 mitral valve, 55 aortic valve, 1 tricuspid, 30 mitro-aortic, 1 mitro-tricuspid and 1 mitro-aorto-tricuspid valve infections. aerococcus viridans was isolated in on ... | 1986 | 3085609 |
| purification and properties of aerococcus viridans lactate oxidase. | lactate oxidase was purified from cells of aerococcus viridans by a procedure which utilized ammonium sulfate fractionation, deae sepharose cl-6b chromatography, and sephadex g-100 chromatography. the final preparation was homogeneous by sds-polyacrylamide gel electrophoresis. the enzyme appears to be a tetramer with a subunit molecular weight of 44,000 and utilizes fmn as a cofactor. the enzyme was highly specific for l-lactate. d-lactate, glycolate, and d,l-2-hydroxybutyrate were not oxidized ... | 1989 | 2818595 |
| [the effect of aerococcus viridans on the properties of salmonellae and staphylococci in vitro]. | according to electron microscopy the repeated contacts of aerococcus viridans 167 (industrial strain for the probiotic aerobact production) with salmonella typhimurium and staphylococcus aureus caused the deep changes of cellular structure, down to bacteriolysis. remaining viable pathogenic cells gave posterity with changed biochemical properties and virulence. s. typhimurium completely lost typical biochemical properties, its mobility and ability to be agglutinated by o-specific salmonella seru ... | 1999 | 10483228 |
| cloning and analysis of the l-lactate utilization genes from streptococcus iniae. | the presence of lactate oxidase was examined in eight streptococcus species and some related species of bacteria. a clone (pgr002) was isolated from a genomic library of streptococcus iniae generated in escherichia coli, containing a dna fragment spanning two genes designated lcto and lctp. we show that these genes are likely to be involved in the l-lactic acid aerobic metabolism of this organism. this dna fragment has been sequenced and characterized. a comparison of the deduced amino acid sequ ... | 1999 | 10508058 |
| molecular and physiological characterization of dominant bacterial populations in traditional mozzarella cheese processing | the development of the dominant bacterial populations during traditional mozzarella cheese production was investigated using physiological analyses and molecular techniques for strain typing and taxonomic identification. analysis of rapd fingerprints revealed that the dominant bacterial community was composed of 25 different biotypes, and the sequence analysis of 16s rdna demonstrated that the isolated strains belonged to leuconostoc mesenteroides subsp. mesenteroides, leuc. lactis, streptococcu ... | 1999 | 10583686 |
| identification of aesculin-hydrolyzing streptococci, lactococci, aerococci and enterococci from subclinical intramammary infections in dairy cows. | aesculin-hydrolyzing, catalase-negative, gram-positive cocci isolated from subclinical intramammary infections in dairy cows were identified to species level using growth characteristics and biochemical activity. the results indicated that the aesculin-hydrolyzing cocci associated with this type of infection are a very heterogenic group. s. uberis strains, including inulin- or beta-glucuronidase-negative isolates, accounted for only about one-third of the collection, and enterococcus faecalis st ... | 1999 | 10591500 |
| caries development and aerococcus viridans. | | 1999 | 10613146 |
| identification of monounsaturated fatty acids of aerococcus viridans with dimethyl disulfide derivatives and combined gas chromatography-mass spectrometry. | location of the double-bond position of monounsaturated fatty acids of aerococcus viridans was accomplished by combined gas chromatography (gc)-mass spectrometry analysis of dimethyl disulfide (dmds) derivatives. the monoenoic fatty acids from whole bacterial cells were converted to methyl esters and then to dmds adducts and analyzed by capillary gc-mass spectrometry. the mass spectra of dmds adducts gave an easily recognizable molecular ion (m+) and two major diagnostic ions attributable to fra ... | 1989 | 2778078 |
| properties and stability of glycerophosphate oxidase isolated from a mutant strain of aerococcus viridans. | the properties of microbial l-alpha-glycerophosphate oxidase (gpo) isolated from a mutant strain of aerococcus viridans dbm 1509 were estimated. the stability at different temperatures and ph were detected. at 4 degrees c the enzyme lost activity during 15 d, at 20 degrees c and 30 degrees c gpo activity decreased during 30 and 25 h, respectively. the highest stability was measured at - 20 degrees c and ph 9. at 4 degrees c the stability was enhanced by the addition of 0.1 m edta or by lyophiliz ... | 2000 | 10747248 |
| interaction of two arginine residues in lactate oxidase with the enzyme flavin: conversion of fmn to 8-formyl-fmn. | two arginine residues, arg-181 and arg-268, are conserved throughout the known family of fmn-containing enzymes that catalyze the oxidation of alpha-hydroxyacids. in the lactate oxidase from aerococcus viridans, these residues have been changed to lysine in two single mutations and in a double mutant form. in addition, arg-181 has been replaced by methionine to determine the effect of removing the positive charge on the residue. the effects of these replacements on the kinetic and thermodynamic ... | 2000 | 11078532 |
| detection of serum thermolabile beta-2 macroglycoprotein (hakata antigen) by enzyme-linked immunosorbent assay using polysaccharide produced by aerococcus viridans. | although a serum thermolabile beta-2 macroglycoprotein (tmg) may play a role in host defense as a lectin, little is known of its related physiological functions, mainly due to a lack of appropriate methods for tracing the functions of tmg. we identified a polysaccharide from aerococcus viridans, psa, which reacts with tmg, and based on this finding, we developed an enzyme-linked immunosorbent assay to trace the functions of tmg. using ethanol precipitation and deae-sepharose and sephacryl s-400 ... | 2001 | 11238239 |
| protein dynamics from chemical shift and dipolar rotational spin-echo 15n nmr. | the partial collapse of dipolar and chemical shift tensors for peptide nh and for the amide nh at cross-link sites in cell wall peptidoglycan, of intact lyophilized cells of aerococcus viridans, indicates nh vector root-mean-square fluctuations of 23 degrees. this result is consistent with the local mobility calculated in typical picosecond regime computer simulations of protein dynamics in the solid state. the experimental root-mean-square angular fluctuations for both types of nh vectors incre ... | 1989 | 2713369 |
| aerococcus urinaehominis sp. nov., isolated from human urine. | phenotypic and phylogenetic studies were performed on an unknown gram-positive catalase-negative coccus isolated from human urine. comparative 16s rrna gene sequencing demonstrated that the organism represents a new subline within the genus aerococcus. the unknown bacterium was readily distinguished from the three currently recognized aerococcus species, aerococcus christensenii, aerococcus urinae and aerococcus viridans, by biochemical tests and electrophoretic analysis of whole-cell proteins. ... | 2001 | 11321115 |
| dna relatedness, phenotypic characteristics, and antimicrobial susceptibilities of globicatella sanguinis strains. | dna-dna reassociation was performed on 15 strains of globicatella sanguinis to compare their taxonomic status with phenotypic characterization. all 15 strains selected for dna-dna reassociation readily met the criteria for species relatedness. the relative binding ratio was 81% or greater at the optimal temperature and 76% or greater at the stringent temperature, and the divergence was less than 3% for all strains hybridized with the type strain. these strains included nine strains from the cent ... | 2001 | 11682529 |
| studies on antagonism between porcine skin bacteria. | strains of staphylococcus warneri, staph. epidermidis and aerococcus viridans isolated from porcine skin were shown to produce substances that inhibited the growth of staph. hyicus. studies of interactions between the inhibitor-producing bacteria and staph. hyicus on cellulose acetate membranes demonstrated a significant reduction (p less than 0.001) of the staph. hyicus population at various stages of its growth cycle, depending on the inhibitor used. inhibitor production profiles in batch cult ... | 1989 | 2666380 |
| urosepticemia and fatal endocarditis caused by aerococcus-like organisms. | two cases of invasive infections with aerococcus-like organisms (alo) are presented: an 81-year-old man with fatal endocarditis and a 63-year-old man with urosepticemia. no antigenic relationship was found between alo and aerococcus viridans (nctc 8251) in crossed immunoelectrophoretic assay. | 1991 | 1815334 |
| [the adhesive and colonizing properties of aerococcus viridans]. | on the basis of experiments carried out in the course of this study the conclusion can be made that one of the mechanisms of the preventive effect of aerococci, the representatives of the indigenous microflora of the gastrointestinal tract, lies in their capacity for adhesion to and colonization of the mucous membrane, this enhancing the resistance of the enteric tract to infections. these properties of anterococci substantiate good prospects of using m-bacterin (live lyophilized aerococcal cult ... | 1991 | 1759533 |
| esophageal diverticulum associated with aerococcus viridans infection in a loggerhead sea turtle (caretta caretta). | a juvenile loggerhead sea turtle (caretta caretta) stranded in gran canaria, spain was necropsied. the turtle was underweight, had sunken eyes, and small amounts of crude oil were in the oral cavity. the most significant lesion was a large esophageal diverticulum at the junction of the esophagus and stomach. the diverticulum was full of gas and green mucoid fluid and a diffuse thick yellow fibrinonecrotic membrane covered the mucosa. the lumen of the diverticulum also contained moderate numbers ... | 2002 | 11838222 |
| penicillin-resistant aerococcus viridans bacteremia associated with granulocytopenia. | aerococcus viridans, a catalase-negative gram-positive coccus rarely causing bacteremia, was isolated from blood cultures of a 52-yr-old man under the granulocytopenic condition. the isolate showed the typical characteristics of a. viridans, i.e., tetrad arrangements in gram stain, positive pyrrolidonyl aminopeptidase (pyr) and negative leucine aminopeptidase (lap) reactions, and no growth at 45 degrees c. the isolate was revealed to be highly resistant to penicillin, erythromycin, clindamycin, ... | 2002 | 11850599 |
| restriction fragment length polymorphism analysis of 16s ribosomal dna of streptococcus and enterococcus species of bovine origin. | twelve bacterial species including streptococcus uberis, s. parauberis, s. agalactiae, s. dysgalactiae, s. bovis, s. mitis, s. salivarius, s. saccharolyticus, enterococcus faecium, e. faecalis, e. avium, and aerococcus viridans were examined for their 16s ribosomal dna fingerprint patterns. oligonucleotide primers complementary to 16s rrna genes were used to amplify by the polymerase chain reaction 16s ribosomal gene fragments from genomic dnas. the molecular sizes of the amplified 16s ribosomal ... | 1992 | 1357000 |
| non-linear slow-binding inhibition of aerococcus viridans lactate oxidase by cibacron blue 3ga. | lactate oxidase (lod) was purified from cells of aerococcus viridans by phase partitioning in triton x-114 (tx-114), ammonium sulphate fractionation and fplc ion exchange chromatography. the purification achieved from a crude extract of a. viridans was 32-fold with a 60% recovery of activity. the isolated enzyme was a true fmn-containing lod in tetrameric form with a subunit molecular weight of 48,000. the km for l-lactate was 175 microm, a 6-fold less value than described in the literature. amo ... | 2001 | 11916135 |
| the ld-carboxypeptidase activity in gaffkya homari. the target of the action of certain beta-lactam antibiotics on the formation of wall-bound peptidoglycan. | | 1978 | 729580 |
| the ld-carboxypeptidase activity in gaffkya homari. the target of the action of d-amino acids or glycine on the formation of wall-bound peptidoglycan. | the effects in vitro of d-amino acids or glycine on the formation of wall-bound peptidoglycan were studied with wall membrane enzyme preparations from gaffkya homari. these amino acids inhibited the incorporation of nascent peptidoglycan into the preformed polymer (e.g. id50 values for d-alanine, d-leucine, and glycine = 5.6 mmol/l, 1.3 mmol/l, and 11 mmol/l, respectively). the inhibition was accompanied by an incorporation of the inhibitor into position 4 of the peptide subunit ala1-dglu2(lys3- ... | 1978 | 729579 |
| serum concentration of hakata antigen, a member of the ficolins, is linked with inhibition of aerococcus viridans growth. | hakata antigen (hakata) is a novel serum glycoprotein that consists of collagen- and fibrinogen-like domains, similar to ficolin/p35. our research suggested that serum hakata may be a target of a polysaccharide (psa) produced by aerococcus viridans. | 2002 | 12367778 |
| identification of aerococcus viridans by means of co-agglutination. | | 1978 | 570351 |
| identification of lactic acid bacteria from spontaneous fermentation of 'almagro' eggplants by sds-page whole cell protein fingerprinting. | in order to complete the previously performed phenotypic identification [sánchez et al., 2000. int. j. food microbiol. 59 (2000) 9], whole cell protein patterns obtained using sodium dodecyl sulphate polyacrylamide gel electrophoresis (sds-page) of 149 lactic acid bacteria (lab) strains, isolated from 'almagro' eggplants spontaneous fermentation, were analysed. phenotypic identification of those strains had revealed the presence of the species lactobacillus (lb) plantarum, lb. pentosus, lb. brev ... | 2003 | 12568758 |
| high-level gentamicin-resistant isolates of oral streptococci and aerococcus from blood specimens. | seventy-nine isolates of oral streptococci and aerococcus obtained from blood specimens between december 1997 and january 2000 were tested for high-level gentamicin resistance. three isolates with minimum inhibitory concentrations (mics) of > or =1024 microg/ml were selected as potential high-level gentamicin-resistant isolates. the dna sequence of the structural gene encoding 6'-acetyltransferase-2"-phosphotransferase in the three high-level gentamicin-resistant isolates was investigated accord ... | 2003 | 12673402 |
| biosynthesis of spin-labeled peptidoglycan: spin-spin interactions. | membrane preparations from gaffkya homari catalyzed the in vitro biosynthesis of soluble uncross-linked spin-labeled peptidoglycan, a uniformly labeled polynitroxide, from the spin-labeled nucleotide udp-murnac-ala-dglu-lys(nepsilon-2,2,5,5-tetramethyl-1-pyrrolin-1-oxyl-3-carbonyl)-dala-dala (i) and udp-glcnac. soluble spin-labeled peptidoglycan was separated from membrane fragments and its spin-labeled precursor by centrifugation and gel filtration. the molecular weight distribution of the poly ... | 1977 | 14677 |
| thermal resistances and lactate and diacetate sensitivities of bacteria causing bologna discolouration. | this report describes the effects of heat, sodium lactate, and sodium diacetate on the viabilities of weissella viridescens atcc 12706, aerococcus viridans mpl-1 and mpl-b, and carnobacterium viridans atcc baa 336. the latter three organisms were isolated from commercial product and have previously been shown to produce green discolourations in cooked cured bologna. w. viridescens was heat resistant in beef bologna (d(60 degrees c)=14.7 min) but not in apt broth. a. viridans and c. viridans were ... | 2003 | 12915033 |
| a biochemical-taxonomic study of a marine micrococcus, gaffkya homari, and a terrestrial counterpart. | | 1956 | 13385431 |
| utilization of purines and their derivatives by gaffkya homari. | | 1957 | 13502309 |
| relationship between purines and folic acid-vitamins in the micrococcus, gaffkya homari. | | 1958 | 13549369 |
| mode of action of azaserine on gaffkya homari. | | 1959 | 13654215 |
| comparative study of gaffkya homari, aerococcus viridans, tetrad-forming cocci from meat curing brines, and the genus pediococcus. | | 1960 | 13815447 |
| interrelationship between temperature and sodium chloride on growth of lactic acid bacteria isolated from meat-curing brines. | goldman, manuel (american meat institute foundation, chicago, ill.), r. h. deibel, and c. f. niven, jr. interrelationship between temperature and sodium chloride on growth of lactic acid bacteria isolated from meat-curing brines. j. bacteriol. 85:1017-1021. 1963.-an elevation of the temperature limit for growth of some pediococcus homari (gaffkya homari) and motile lactobacillus strains could be effected by the addition of sodium chloride to the growth medium. at the optimal temperature for grow ... | 1963 | 14043988 |