serum bactericidal activity in the horseshoe crab, limulus polyphemus.serum from the horseshoe crab, limulus polyphemus, was examined for bactericidal activity against five species of bacteria. greatest activity was found against pseudomonas putida and flavobacterium sp.; with the former, serum dilutions as high as 1:20 were capable of reducing viable counts by 50% within 2 h. bactericidal activity of a significantly lesser magnitude was demonstrated against serratia marcesencs and salmonella minnesota. no killing was seen when the lobster pathogen aerococcus viri ...197611188
aldolases of the lactic acid bacteria. demonstration of immunological relationships among eight genera of gram positive bacteria using an anti-pediococcal aldolase serum.reciprocal qualitative and quantitative immunological experiments employing an anti-pediococcus cerevisiae aldolase serum confirmed many of the interspecific relationships demonstrated previously among lactic acid bacteria with antisera prepared against the streptococcus faecalis fructose diphosphate aldolase. the extent of immunological relatedness observed between the lactobacillus and pediococcus aldolases was markedly gses indicating that the pediococci share closer phylogenetic ties with th ...197665160
identification of aerococcus viridans by means of co-agglutination. 1978570351
airborne bacteria in an urban environment.samples were taken at random intervals over a 2-year period from urban air and tested for viable bacteria. the number of bacteria in each sample was determined, and each organism isolated was identified by its morphological and biochemical characteristics. the number of bacteria found ranged from 0.013 to 1.88 organisms per liter of air sampled. representatives of 19 different genera were found in 21 samples. the most frequently isolated organisms and their percent of occurence were micrococcus ...1978677875
[bacterial flora of the conjunctival sac of the horse].the aa. report the results of taxonomic research conducted on the conjunctival sac of 59 horses for identification of the present bacterial flora. in the controlled animals, it was observed, at the level of the considered niche, a community constituted of normal bacterial populations, but not autochtonous in the significance they attributed from dubos et al., relative to the characterization of the indigenous microbiota of the intestine. the isolated normal bacterial flora seems to be constitute ...1976788654
[therapeutic effect of lividomycin on infections of the respiratory tract (author's transl)].therapeutic effect of lividomycin was observed in patients with infections of the respiratory tract (infections in association with bronchiectasis, mixed infections of cavities after negative conversion of tubercle bacilli, pneumonia, and so forth). significant improvement of clinical symptoms was observed in 15 cases and no improvement in 4 cases. lividomycin was administered at a dose of 1 g/day for 3 to 15 days. staphylococcus aureus (9 cases), klebsiella pneumoniae (1 case), alpha-hemolytic ...1975807755
serological grouping of virulent and avirulent strains of the lobster pathogen aerococcus viridans.virulent strains of aerococcus viridans (formerly gaffkya homari) are the aetiologic agents of gaffkemia, a septicaemic disease of the american lobster (homarus americanus). the virulent and avirulent forms of this bacterium, previously thought to be taxonomically indistinguishable, have been differentiated by serological studies. antisera were produced in rabbits using autoclaved bacteria as antigens. reactions were measured by agglutination tests using microtitre techniques and an antigenic sc ...1977874455
4-o-[(s)-1-carboxyethyl]-d-glucose: a component of the extracellular polysaccharide material from aerococcus viridans var. homari. 19761009540
endocarditis and osteomyelitis caused by aerococcus viridans. 19761084468
stereoselective epoxidation of phenyl allyl ether by alkene-utilizing bacteria.eighteen newly isolated ethene- and propene-utilizing bacteria were screened for the ability to produce phenyl glycidyl ether, a common precursor for the synthesis of beta blockers, from phenyl allyl ether. these organisms included aerococcus, alcaligenes, micrococcus and staphylococcus spp. and a variety of gram-negative, gram-positive and gram-variable mesophilic rods/coccobacilli not yet identified. the majority of ethene- and propene-grown cultures (14 strains) accumulated phenyl glycidyl et ...19921368499
phylogenetic analysis of some aerococcus-like organisms from urinary tract infections: description of aerococcus urinae sp. nov.partial 16s ribosomal ribonucleic acid sequences of five aerococcus-like organisms originally isolated from patients with urinary tract infections were determined using reverse transcriptase in order to clarify their taxonomic position. analysis of the sequence data revealed that the clinical isolates represent a hitherto unknown line of descent within the genus aerococcus. a new species, aerococcus urinae, is proposed for these isolates. the type strain is ncfb 2893.19921373437
description and evaluation of the semiautomated 4-hour rapid id 32 strep method for identification of streptococci and members of related genera.the rapid id 32 strep system (biomérieux, la balme les grottes, france) is a new system which allows the identification in 4 h of most streptococci and members of related genera encountered in medical and veterinary bacteriology. four hundred thirty-three isolates first identified by conventional methods and belonging to the genera streptococcus, lactococcus, enterococcus, aerococcus, gemella, leuconostoc, erysipelothrix, gardnerella, and listeria were tested. overall, rapid id 32 strep correctl ...19921400965
identification of some uncommon monounsaturated fatty acids of bacteria.location of the double-bond position of monounsaturated fatty acids of various bacteria was accomplished with combined gas chromatography-mass spectrometry analysis of dimethyl disulfide (dmds) derivatives. the monoenoic fatty acids from whole cells were converted to methyl esters and then to dmds adducts and analyzed by capillary gas chromatography-mass spectrometry. the mass spectra of dmds adducts gave an easily recognizable molecular ion and two major diagnostic ions attributable to fragment ...19921401029
culture media for non-sporulating gram-positive food spoilage bacteria.the spoilage association especially of protein-rich foods can be dominated by gram-positive bacteria, notably lactic acid bacteria (lab) which affect vacuum packaged refrigerated processed meats and some dairy products. new food ecosystems are being created by novel packaging and processing technologies, resulting in spoilage associations differing from those previously reported. in addition, improvement in identification methods, allow the detection and isolation of 'novel' bacterial groups, e. ...19921486021
rapid identification of aerococcus viridans using the polymerase chain reaction and an oligonucleotide probe.a polymerase chain reaction/oligonucleotide probe method was developed for the specific identification of the gram-positive bacterium aerococcus viridans. primers for the enzymatic amplification reaction were designed from specific sequences within the 16s rrna. the method was also highly sensitive and 10 cfu of a. viridans could be detected in 5 h although the reliability of detection was poor in mixed cultures with escherichia coli.19921516808
[the adhesive and colonizing properties of aerococcus viridans].on the basis of experiments carried out in the course of this study the conclusion can be made that one of the mechanisms of the preventive effect of aerococci, the representatives of the indigenous microflora of the gastrointestinal tract, lies in their capacity for adhesion to and colonization of the mucous membrane, this enhancing the resistance of the enteric tract to infections. these properties of anterococci substantiate good prospects of using m-bacterin (live lyophilized aerococcal cult ...19911759533
urosepticemia and fatal endocarditis caused by aerococcus-like organisms.two cases of invasive infections with aerococcus-like organisms (alo) are presented: an 81-year-old man with fatal endocarditis and a 63-year-old man with urosepticemia. no antigenic relationship was found between alo and aerococcus viridans (nctc 8251) in crossed immunoelectrophoretic assay.19911815334
aerococcus-like organism, a newly recognized potential urinary tract pathogen.during the last 10 to 15 years, aerococcus-like organisms have been isolated from urinary tract specimens from hospitalized patients at bispebjerg hospital, copenhagen, denmark, with signs of urinary tract infection, and a single strain has been deposited at the national collection of type cultures (nctc 12142). an additional 63 isolates from the urine specimens of as many patients with suspected urinary tract infection have been identified from seven departments of clinical microbiology in denm ...19912056040
structural studies of the capsular polysaccharide from aerococcus viridans var. homari.the capsular polysaccharide from aerococcus viridans var. homari has been investigated, using n.m.r. spectroscopy, methylation analysis, and specific degradations as the main methods. the polysaccharide is composed of tetrasaccharide repeating-units having the following structure. (formula; see text) in this structure, d-quin stands for 2-amino-2,6-dideoxy-d-glucose (quinovosamine). two of the three acidic sugars found, namely, l-altruronic acid and 4-o-[(s)-1-carboxyethyl]-d-glucose, have not b ...19902085808
the phylogeny of aerococcus and pediococcus as determined by 16s rrna sequence analysis: description of tetragenococcus gen. nov.the phylogenetic interrelationships of the genera pediococcus and aerococcus were investigated using reverse transcriptase sequencing of 16s rrna. the genus pediococcus was found to be phylogenetically heterogeneous. the four species p. acidilactici, p. damnosus, p. parvulus and p. pentosaceus formed a phylogenetically distinct group. within this pediococcal cluster, p. acidilactici was closely related to p. pentosaceus whereas p. damnosus showed a specific relationship with p. parvulus. the spe ...19902227360
phenotypic characterization, cellular fatty acid composition, and dna relatedness of aerococci and comparison to related genera.aerococci can be misidentified as streptococci, enterococci, pediococci, lactococci, or leuconostocs. to distinguish the genus and determine if another species is needed in the present taxon, we analyzed 37 aerococci for cellular fatty acids and compared them with 377 strains of gram-positive cocci, including the species type strains from each of the related genera. the cellular fatty acid profile of aerococci was distinguishable from other genera. two relatively novel fatty acids found in the a ...19902324269
minimum inhibitory concentrations and disk diffusion zone diameter for selected antibiotics against streptococci isolated from bovine intramammary infections.the antimicrobial susceptibility to 11 antibiotics was determined of 358 streptococcal isolates representing six species was determined. all isolates were from milk samples obtained from clinical or subclinical cases of bovine mastitis. agar disk diffusion zone diameters and minimum inhibitory concentrations were obtained for all isolates. of the antibiotics tested that are available for mastitis therapy, cephalothin, penicillin, ampicillin, novobiocin, and erythromycin demonstrated acceptable a ...19902365883
[the lactate oxidase activity of aerococcus viridans cultures].the oxidase activity of aerococcus viridans is a result of the functioning of aerobic nad-independent lactate dehydrogenase. two fractions of flavin-containing protein which oxidize d- and l-isomers of lactic acid are revealed during the enzymic complex electrophoresis in paag. the enzymic complex and eluates of both fractions possess the antagonistic activity relative to 27 test-cultures of bacteria.19892622398
[the effect of aerococcus viridans--the basis of the new therapeutic-prophylactic preparation m-bacterin--on the biological properties of staphylococcus aureus].representatives of the normal microflora from genus aerococcus, in particular strain aerococcus viridans 167 isolated from breast milk are studied for their effect on biological properties of staphylococcus aureus in vitro and in vivo. it is established that the number of viable cells of the staphylococcus cultivated in the presence of antagonists in the beef-extract agar decreases progressively with each following passage, the population dying after the seventh-eight passage. electronograms fix ...19892622400
protein dynamics from chemical shift and dipolar rotational spin-echo 15n nmr.the partial collapse of dipolar and chemical shift tensors for peptide nh and for the amide nh at cross-link sites in cell wall peptidoglycan, of intact lyophilized cells of aerococcus viridans, indicates nh vector root-mean-square fluctuations of 23 degrees. this result is consistent with the local mobility calculated in typical picosecond regime computer simulations of protein dynamics in the solid state. the experimental root-mean-square angular fluctuations for both types of nh vectors incre ...19892713369
genetic basis of antibiotic resistance in aerococcus viridans.resistance to at least one of the following antibiotics was found in eight wild-type strains of aerococcus viridans: erythromycin (six strains), tetracycline and minocycline (five strains), chloramphenicol (one strain), and high levels of streptomycin (one strain). none of the strains transferred any of their antibiotic resistance markers into streptococcal, enterococcal, or a. viridans recipients by conjugation. by dna-dna hybridization experiments, the ermb gene of transposon tn917, of enteroc ...19892729946
aerococcus-like organism--an unnoticed urinary tract pathogen.aerococci are only occasionally isolated from urinary tract specimens. a 4-month' survey revealed 29 patients suspected of urinary tract infection (uti), from whom an aerococcus-like organism (alo) was isolated in significant numbers from urinary tract specimens and singly in 11 cases. approximately 0.8% of all urinary tract specimens examined during the period yielded growth of alo. the median age of patients was 75 years. half of the patients were found to have conditions predisposing to uti. ...19892736106
identification of monounsaturated fatty acids of aerococcus viridans with dimethyl disulfide derivatives and combined gas chromatography-mass spectrometry.location of the double-bond position of monounsaturated fatty acids of aerococcus viridans was accomplished by combined gas chromatography (gc)-mass spectrometry analysis of dimethyl disulfide (dmds) derivatives. the monoenoic fatty acids from whole bacterial cells were converted to methyl esters and then to dmds adducts and analyzed by capillary gc-mass spectrometry. the mass spectra of dmds adducts gave an easily recognizable molecular ion (m+) and two major diagnostic ions attributable to fra ...19892778078
purification and properties of aerococcus viridans lactate oxidase.lactate oxidase was purified from cells of aerococcus viridans by a procedure which utilized ammonium sulfate fractionation, deae sepharose cl-6b chromatography, and sephadex g-100 chromatography. the final preparation was homogeneous by sds-polyacrylamide gel electrophoresis. the enzyme appears to be a tetramer with a subunit molecular weight of 44,000 and utilizes fmn as a cofactor. the enzyme was highly specific for l-lactate. d-lactate, glycolate, and d,l-2-hydroxybutyrate were not oxidized ...19892818595
comparison of physiologic tests used to identify non-beta-hemolytic aerococci, enterococci, and streptococci.twenty-one reference strains and 88 clinical isolates of aerococcus, enterococcus, and streptococcus species were tested for reactions in the rapid strep (rs) and modified conventional tests. we conclude that some but not all of the tests in the rs system could be used to substitute for conventional tests. rs tests for hydrolysis of arginine, esculin, l-pyrrolidonyl-naphthylamide, production of acetyl methyl carbinol (voges-proskauer), and fermentation of arabinose, lactose, mannitol, raffinose, ...19873117840
[the action of hydrogen peroxide produced by aerococcus viridans on escherichia coli and bacillus subtilis]. 19873150491
relationship between in vitro susceptibility test results for chloramphenicol and production of chloramphenicol acetyltransferase by haemophilus influenzae, streptococcus pneumoniae, and aerococcus species.haemophilus influenzae, streptococcus pneumoniae, and aerococcus species were tested for susceptibility to chloramphenicol by standard broth microdilution and disk-diffusion methods. mics and zone diameter breakpoints were correlated with production of chloramphenicol acetyltransferase (cat). a comparison of mics and zone diameters indicated that the interpretative criteria for h. influenzae and s. pneumoniae should be an mic of less than or equal to 4 micrograms/ml or a zone diameter greater th ...19883266221
ofloxacin for prevention of bacterial infections in granulocytopenic patients.we studied the potential value of oral ofloxacin (200 mg twice daily) for selective decontamination and infection prevention in 40 granulocytopenic patients with acute leukemia, blast crisis of chronic myelogenous leukemia, hairy cell leukemia or severe aplastic anemia. the quality of selective decontamination was acceptable with rapid elimination of enterobacteriaceae from the alimentary tract, only a slight decrease in concentrations of anaerobes in faeces, and a small number of newly acquired ...19873481358
characterization of peptidoglycan stem lengths by solid-state 13c and 15n nmr.lyophilized whole cells of aerococcus viridans (gaffkya homari) grown on a synthetic medium containing d-[2-13c, 15n]ala, or containing both l-[1-13c]lys and d-[15n]ala, have been examined by double cross-polarization magic-angle spinning 13c and 15n nuclear magnetic resonance. results from the double-labeled alanine experiment confirm the absence of metabolic scrambling of alanine by a. viridans. results from the combined single-label experiment can be used to count directly the number of adjac ...19863729935
new test system for identification of aerococcus, enterococcus, and streptococcus species.a total of 244 strains of aerococcus, enterococcus, and streptococcus species were tested by the rapid str system (innovative diagnostic systems inc., atlanta, ga.) for identification. strains were identified without additional tests or with additional conventional tests suggested by the ids compendium manual. our data indicate that the rapid str system identifies 89% of the beta-hemolytic streptococcus species if serological procedures are used in conjunction with the rapid physiological proced ...19863771750
cross-reactions between alpha-streptococci and omniserum, a polyvalent pneumococcal serum, demonstrated by direct immunofluorescence, immunoelectroosmophoresis, and latex recent years several groups have used serological methods to demonstrate pneumococcal capsular antigens in sputum. in the present study 123 strains of alpha-hemolytic streptococci (including 97 strains from sputum or pharyngeal specimens) were tested for cross-reactions with a polyvalent antipneumococcal serum (omniserum). representatives of the following species were included: streptococcus bovis, s. equinus, s. intermedius, s. lactis, s. milleri, s. mitis, s. mutans, s. sobrinus, s. salivar ...19853889046
solid-state 15n nmr studies of the effects of penicillin on cell-wall metabolism of aerococcus viridans (gaffkya homari).lyophilized whole cells and isolated cell walls from aerococcus viridans (gaffkya homari) grown on a synthetic medium containing benzylpenicillin, and either l-[epsilon-15n]lysine or 15n-ammonium ion as the only source of label, have been studied using cross-polarization magic-angle spinning 15n nuclear magnetic resonance. the lysine is incorporated directly into protein and cell-wall peptidoglycan and was used to measure cell-wall cross-links. the ammonium ion acts as a non-specific label monit ...19853977898
septic arthritis due to aerococcus viridans.a 20-year-old woman was found to have septic arthritis of the hip due to aerococcus viridans. this organism closely resembles streptococcus viridans, but forms gram positive tetrads rather than chains in broth media. the organism has been reported rarely to cause endocarditis and one case of osteomyelitis has been observed. to our knowledge septic arthritis due to aerococcus viridans has not been previously reported.19854087248
wall peptidoglycan in aerococcus viridans strains 201 evans and atcc 11563 and in gaffkya homari strain atcc 10400. 19714254570
deoxyribonucleic acid homology among strains of the lobster pathogen 'gaffkya homari' and aerococcus viridans. 19744822121
recognition of aerococcus viridans by the clinical microbiologist. 19705507757
aerococcus-like organisms isolated from human infections.ten strains of gram-positive cocci isolated from patients with endocarditis and other infections, though resembling enterococci, proved to belong to the aerococcus-pediococcus-gaffkya group of bacteria.19675602566
[greening of cooked ham by aerococcus viridans and study of its thermic resistance]. 19685731455
[cefotiam passage into the maxillary sinus tissues].clinical study was made on cefotiam (ctm) and the following results were obtained. tissue concentrations of ctm were determined 1 hour after intravenous injection (ctm 1 g) in chronic sinusitis and maxillary cyst. concentrations of ctm were 16.53 micrograms/g, 13.26 micrograms/g in mucosa of the maxillary sinus, maxillary cyst, respectively. antibacterial activity of ctm was measured on escherichia coli, staphylococcus epidermidis, aerococcus, acidaminococcus fermentans, peptostreptococcus anaer ...19836325743
acute meningitis in early childhood caused by aerococcus viridans. 19836404411
numerical taxonomy of streptococcus.a numerical taxonomic study of strains of streptococcus, together with representatives of allied genera, showed 28 reasonably distinct phenons. the major areas, with their phenons, were: (a) enterococcal species group (s. faecalis, s. faecium, 's. avium' and a proposed new species 's. gallinarum'); (b) paraviridans species group (s. bovis, s. equinus, s. salivarius, 's. casseliflavus', s. mutans, s. raffinolactis and an unidentified oral group i); (c) lactic species group (s. lactis including s. ...19836409982
evaluation of the rapid strep system for the identification of clinical isolates of streptococcus species.a total of 247 strains of streptococci isolated from humans were tested for identification in the rapid strep system. the identification rates and identification levels were different for each streptococcus species. our data indicate that the rapid strep system will identify nearly all the beta-hemolytic streptococcus species if serological procedures are used in conjunction with the rapid physiological procedures. of the group d streptococci, 98% of the enterococci and 95% of the non-enterococc ...19846511872
aerococcus viridans in the hospital environment.aerococcus viridans has been described as an airborne organism prevalent in occupied rooms. it has also been described as an organism having many characteristics that might cause it to be confused with streptococci or staphylococci, and this may account for the fact that the presence of a. viridans has not been reported in the hospital environment or in clinical specimens. swab specimens were taken from 47 objects in 11 different areas in a local hospital, cultured overnight in trypticase soy br ...19685649865
direct measurement of peptidoglycan cross-linking in bacteria by 15n nuclear magnetic resonance.cross-polarization magic angle spinning 9.12-mhz 15n nuclear magnetic resonance was used to measure the extent of peptidoglycan cross-linking within intact bacterial cells and isolated cell walls of aerococcus viridans atcc 10400 grown in the presence of l-[epsilon-15n]lysine. a value of 49% for the cross-linking index was found for normal cells, while for those grown in the presence of low levels of penicillin g (0.1 micrograms/ml), the cross-linking index was reduced to 35%.19836885802
classification of bacteria from commercial egg washers and washed and unwashed eggs.a total of 432 bacterial isolates from washed and unwashed eggs, egg-washer surfaces, and washwaters from five egg-grading plants in maryland and southeastern pennsylvania were classified. counts on equipment surfaces showed considerable variation from plant to plant, reflecting care used in cleaning. unwashed eggs had a higher percentage of gram-positive cocci (71%), and isolates included streptococcus faecalis, aerococcus, and escherichia coli, which were not isolated from equipment surfaces a ...19806893532
nutritional requirements for growth of aerococcus viridans. 19705489060
[microflora in swine slurry as a parameter in determining the efficiency of deodorizing treatment. i. continuous and discontinuous aeration treatment].liquid manure from the pig, fresh and stored, was treated by continuous and discontinuous aeration. the purpose was to achieve a biologically stabilized and deodorized organic material. the samples which were aerated intermittently had good decomposition rates for nitrogen compounds and even so good deodorization effects. in spite of a minimum of dissolved oxygen of appr. 0.2 mg/l, good deodorization could be achieved by intermittent aeration for 23 days. simultaneous processes of nitrification ...19854050182
solid-state 13c and 15n nuclear magnetic resonance studies of alanine metabolism in aerococcus viridans (gaffkya homari).transport and metabolism of d- and l-alanine by aerococcus viridans (gaffkya homari) have been studied using cross-polarization magic-angle spinning 13c and 15n nmr spectroscopy of lyophilized whole cells, isolated cell walls, and crude extracts. for equimolar concentrations in the growth medium, about 10 times more d-alanine than l-alanine is transported into the cells. examination of cells labeled with d-[13c]alanine and l-[epsilon-15n]lysine by double cross-polarization magic-angle spinning 1 ...19853972803
[stomatococcus mucilaginosus: cultural and biochemical properties of 100 strains isolated from the orl area].a study of the growth characteristics and 80 biochemical tests using api system micromethods (api-50ch, api-20e, api-zym) was carried out on 100 strains of stomatococcus mucilaginosus isolated from the human oral cavity. seventy-seven other enzymatic tests from the api system (ap-arylamidases, osidases and esterases) were also performed on 10 of these strains. the results of these tests were compared to those of 24 type strains of micrococcaceae and aerococcus viridans. a simple identification s ...19853890697
comparative evaluation of the api 20s and automicrobic gram-positive identification systems for non-beta-hemolytic streptococci and aerococci.the api 20s system (analytab products, plainview, n.y.) and the automicrobic gram-positive identification system (gpi; vitek systems, hazelwood, mo.) were evaluated for their capacity to identify the non-beta-hemolytic streptococci and aerococci to the species level. the 20s system identified 86% (six of seven strains) of nonhemolytic group b streptococci, whereas 100% of the same group b streptococcal strains were correctly identified by the gpi system. with both systems 99% (134 of 135 strains ...19853886690
effects of metals on the chemiluminescent response of rainbow trout (salmo gairdneri) phagocytes.quantification of an induced chemiluminescent (cl) response in phagocytes is currently being evaluated as an indicator system for determining those environmental pollutants that may predispose fish to disease. a cl assay was developed using phagocytes from the pronephros of rainbow trout (salmo gairdneri). the cl response of phagocytes to phorbol myristate acetate, a chemical inducer of cl, was shown to be dose-dependent. the response to five species of bacteria was also evaluated. staphylococcu ...19863765345
effect of cultivation conditions on glycerophosphate oxidase production by a mutant strain of aerococcus viridans.the effect of different concentrations of carbon source (lactose) and inducer (glycerol) on biomass and glycerophosphate oxidase (gpo) production by mutant strain of aerococcus viridans 1509 was tested. the best combination of lactose and glycerol concentrations for good enzyme productivity was 0.5% lactose and 2.5% glycerol. further improvement of gpo levels was achieved after scaling-up of the bioprocess and cultivation of the cells in a 3 liter laboratory bioreactor. using 4.5 x 10(-5) m3 s-1 ...19957576349
[analysis of 1116 strains of pathogens isolated from infected burn wounds].we report the analysis of 1,116 strains of pathogens isolated from infected burn wounds of 536 patients hospitalized from 1989 to 1991. from the 1,116 strains of pathogens, 39 species of aerobes and fungi were found, including 217 strains of staphylococcus aureus, 208 strains of pseudomonas aeruginosa and 119 strains of acinetobacter calcoaceticus. the positive rates of the above three bacteria were 19.4%, 18.6% and 10.6% respectively. some opportunistic pathogens, such as bacillus cercus, aeroc ...19957600434
development of a polymerase chain reaction test for specific identification of the urinary tract pathogen aerococcus urinae.a polymerase chain reaction test was developed for identification of the gram-positive urinary tract pathogen aerococcus urinae. oligonucleotide primers were based on highly specific sequences within the small-subunit rrna gene. a confirmatory test based on hybridization of the amplified products to a highly specific internal probe was also developed.19937684752
aerococcus bacteremia associated with granulocytopenia.two cases of bacteremic infection due to aerococci in granulocytopenic patients with acute leukemia and oral mucositis are described. strains isolated from blood cultures of both patients were resistant to the antibiotics given orally for prophylaxis. one patient died in septic shock; surveillance cultures from oral washings in the other repeatedly showed high concentrations of aerococci. aerococci were also isolated from surveillance cultures taken from 5 of 17 other patients with acute leukemi ...19873481576
septicaemia due to aerococcus a case of septicaemia, aerococcus urinae was isolated from the blood. the patient had undergone operation on the prostate gland. the urine was sterile.19947747103
a survey of aerobic bacteria and fungi in the feces of healthy psittacine birds.fecal samples from 61 clinically healthy psittacine birds of a wide variety of species were cultured for bacteria and fungi. the most common bacterial isolates were gram-positive bacilli, which were recovered from 60 of the 61 birds. these organisms included lactobacillus, bacillus, corynebacterium, and streptomyces. gram-positive cocci, cultured from the feces of 21 of the birds, included staphylococcus epidermidis, streptococcus spp., aerococcus spp., and micrococcus spp. only 6 of the 61 psit ...19883382379
growth of enterococcus, lactococcus and streptococcus strains and environmental isolates in liquid media and their reactions on beea.growth of known species of enterococcus, lactococcus and streptococcus and aerococcus viridans in selective and nonselective liquid media routinely used to enumerate faecal streptococci was measured optically at different temperatures. growth of environmental isolates was measured in some of these media. growth of the reference strains on bile esculin azide agar at elevated incubation temperatures was tested. the results revealed only minor differences between media but strong influence of incub ...19947811573
aerococcus viridans endocarditis. case report. 19947875897
[the stimulating effect of aerococcus viridans on phagocytosis and antibody formation]. 19947879500
gram-positive cocci isolated from slaughtered poultry.gram-positive cocci were found in all meat samples of poultry slaughtered and processed for retail sale, at incidence rates ranging from 10(2) cfu/ml to 1.35 x 10(6) cfu/ml and a mode between 8 x 10(5) and 9 x 10(5) cfu/ml for 75% of the samples. the 93 isolated strains were identified as belonging to the following species: enterococcus faecalis (48 strains), e. faecium (16), e. avium (7), e. durans (4), aerococcus viridans (10), streptococcus morbillorum (2), s. salivarius (1), s. sanguis (1), ...19947921897
serous otitis media (s.o.m.). a bacteriological study of the ear canal and the middle ear.a bacteriological study of the middle-ear effusions and the ear canals in children with chronic serous otitis media (s.o.m.) was performed. sixty-eight children (127 ears) were investigated. from this study it appeared that cleansing of the ear canal with 0.5% chlorhexidine in 70% ethanol for 30 s is partially effective; micro-organisms (diptheroids, staphylococcus epidermidis) could still be isolated in 29%. cleansing of the ear canal decreases the incidence of middle-ear fluid contamination by ...19883209359
phylogenetic analysis of some aerococcus-like organisms from clinical sources: description of helcococcus kunzii gen. nov., sp. nov.16s rrna gene sequencing studies were performed on some unusual gram-positive catalase-negative cocci of unknown taxonomic position isolated from human clinical sources. comparative analysis of the sequence data demonstrated that the clinical isolates represent a hitherto-unknown line of descent within the low-g+c-content gram-positive bacteria. on the basis of the phylogenetic findings and the phenotypic distinctiveness of the organisms, it is proposed that they be classified in a new genus, he ...19938347503
presumptive fecal streptococci in environmental samples characterized by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis.the use of fecal streptococci as fecal indicators requires better knowledge of the ecology of these bacteria. we isolated 371 presumptive fecal streptococci from environmental samples--domestic wastewater, forest industry wastewater, contaminated surface and seawater, well water, cow dung, bird droppings, and pristine waters--and clustered them according to their protein profiles in one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis. some clusters could be tentati ...19938357252
l-lactate oxidase and l-lactate monooxygenase: mechanistic variations on a common structural of l-lactate oxidase from aerococcus viridans are described. the gene encoding the enzyme has been isolated. from its cdna sequence the amino acid sequence has been derived and shown to have high similarity with those of other enzymes catalyzing oxidation of l-alpha-hydroxy acids, including flavocytochrome b2, lactate monooxygenase, glycolate oxidase, mandelate dehydrogenases and a long chain alpha-hydroxy acid oxidase. the enzyme is expressed in escherichia coli, and is a flavoprotei ...19958589073
bacteremia/septicemia due to aerococcus-like organisms: report of seventeen cases. danish alo study group.some aerococcus-like organisms (alos) have recently been described in denmark. the bacteria were originally isolated from the urine of elderly patients with urinary tract infections. since 1987, we have identified 17 cases of bacteremia/septicemia in which alos have been isolated in pure culture of blood; we report the data from these cases. six of the patients presented with endocarditis, eight presented with urosepticemia, and three presented with septicemia. in all but one of the reports, the ...19958645844
atmospheric growth requirements for alloiococcus species and related gram-positive cocci.the growth of alloiococcus otitis under different atmospheres and nutritional conditions was studied. the growth rates of 25 strains of gram-positive cocci representing five genera on heart infusion agar plates containing 5% rabbit blood and on brucella agar plates with and without sheep blood under aerobic, increased co2, and anaerobic atmospheres were compared. eight strains of alloiococci plated on heart infusion agar with rabbit blood and on brucella sheep blood agar grew under aerobic and c ...19968815077
penicillin-resistant aerococcus viridans bacteremia in a child receiving prophylaxis for sickle-cell disease. 19968838213
aerococcus-like organisms: use of antibiograms for diagnostic and taxonomic purposes.recently, some aerococcus-like organisms (alos), isolated from urine and blood of elderly patients with urinary tract infection, have been described. in this study alos and related taxons were tested for susceptibility by agar diffusion and agar dilution methods to 15 selected antimicrobial agents for diagnostic and taxonomic considerations. alos were susceptible to a wide range of antimicrobials including beta-lactams, but resistant to aminoglycosides, sulphonamides, trimethoprim and nalidixic ...19968877539
teat skin normal flora and colonization with mastitis pathogen inhibitors.isolates of bacteria from normal teats were used to attempt colonization of teats of dry cows or neonatal calves. isolates for inoculation were chosen on the basis of ability to inhibit mastitis pathogens in vitro, with the ultimate goal of in vivo inhibition of mastitis pathogens at the teat surface. three bacterial normal flora isolates (corynebacterium xerosis, bacillus sp. and aerococcus viridans) persisted less than 10 days on the teats of dry cows. the fourth isolate, staphylococcus homini ...19883188374
conversion of l-lactate oxidase to a long chain alpha-hydroxyacid oxidase by site-directed mutagenesis of alanine 95 to glycine.a mutant form of l-lactate oxidase (lox) from aerococcus viridans in which alanine 95 was replaced by glycine was constructed as a mimic of l-lactate monooxygenase but proved instead to be a mimic of the long chain alpha-hydroxyacid oxidase from rat kidney. a95g-lox keeps oxidase activity with l-lactate at the same level as wild type lox but has much enhanced oxidase activity with longer chain l-alpha-hydroxyacids, alpha-hydroxy-n-butyric acid, alpha-hydroxy-n-valeric acid, etc., and also the ar ...19968910450
[gram-positive, catalase negative cocci isolated from clinical material--identification and drug sensitivity of strains].4608 clinical specimens (including 1641 samples of blood) for bacteriological examination were obtained from patients hospitalized in different wards of national clinical hospital no 1 in warsaw. strains were cultured, identified and their sensitivity to antibacterial drugs was determined during 14 months with the use of semi-automated api (atb-plus) system (biomérieux, france). strains of gram-positive, catalase-negative cocci were separated from all cultured strains. they belonged to the gener ...19968927737
[indications and results of surgery in native valve infectious endocarditis. apropos of 104 surgically-treated cases].from 1972 to 1984, 104 cases of aortic valve infectious endocarditis were treated surgically. the average age of the patients was 40 years and the majority were men (69/104). forty patients had no previous cardiac disease; 44 patients had documented valvular heart disease, which was unlikely in the remaining 20 patients. there were 16 mitral valve, 55 aortic valve, 1 tricuspid, 30 mitro-aortic, 1 mitro-tricuspid and 1 mitro-aorto-tricuspid valve infections. aerococcus viridans was isolated in on ...19863085609
[the biological properties of aerococcus antagonists, representatives of human microbiocenoses].the paper deals with the data on biology of aerococcus, a slightly studied group of microorganisms. physiological-biochemical properties of aerococcus are described, data of their distribution in nature are given. peculiar attention is paid to the estimate of the role of aerococcus in human microbiocenoses. as a result of the profound and all-round study of this group of microorganisms the authors have developed new bacterial drug "a-bakterin" based on the aerococcus strain. data presented about ...19949181979
teicoplanin for therapy of gram-positive infections in neutropenic patients.teicoplanin was evaluated in 20 febrile neutropenic patients as additional treatment for suspected gram-positive infections after inadequate response to initial empiric ceftazidime monotherapy. five patients with primary septicaemia, four with secondary septicaemia, 12 with localized infections and three with pyrexia of unknown origin were treated with teicoplanin (200 mg bolus intravenously, once daily after a 400 mg loading dose), whilst ceftazidime (2 g, 8-hourly, 30 min infusions) was contin ...19872964418
a comparison of the identification of group a streptococci and enterococci by two rapid pyrrolidonyl aminopeptidase a streptococci and enterococci can be differentiated from other streptococci by their ability to cleave l-pyrrolidonyl-beta-napthylamide (pyr). the authors evaluated two pyrrolidonyl aminopeptidase (pyrase) systems--minitek (bbl microbiology systems, cockeysville, md) and identicult-ae (scott laboratories, inc., fiskeville, ri)--for the presumptive identification of group a streptococci and enterococci. eighty-three group a streptococci, 77 beta-hemolytic non-group a streptococci, 74 enter ...19882899393
enzyme characteristics of beta-d-galactosidase- and beta-d-glucuronidase-positive bacteria and their interference in rapid methods for detection of waterborne coliforms and escherichia coli.bacteria which were beta-d-galactosidase and beta-d-glucuronidase positive or expressed only one of these enzymes were isolated from environmental water samples. the enzymatic activity of these bacteria was measured in 25-min assays by using the fluorogenic substrates 4-methylumbelliferyl-beta-d-galactoside and 4-methylumbelliferyl-beta-d-glucuronide. the enzyme activity, enzyme induction, and enzyme temperature characteristics of target and nontarget bacteria in assays aimed at detecting colifo ...19989501441
l-lactate oxidase from aerococcus viridans crystallized as an octamer. preliminary x-ray studies.crystals of flavoenzyme l-lactate oxidase from aerococcus viridans (lox) have been obtained that diffract to 3.0 a resolution (p2(1)2(1)2(1), a = 118.4 a, b = 138.4 a, c = 194.6 a). crystallographic studies suggest that the enzyme may exist as an octameric form with non-crystallographic two- and four-fold axes in the center of the octamer. the four-fold axis makes the tetramer tight, and the tetramers lie upon one another by the two-fold axis.19989672750
[a biological model study of the effect of aerococcus viridans on pathogenic bacteria].morphological researches studies showed that the treatment of infected skin wounds with aerobact quickly eliminated inflammation, promoted regeneration of damaged tissues, that actively stimulated formation of granulations. a decrease of s. aureus virulence which completely or partially loose lethal and necrotic activity is one of the reasons of the observed phenomenon. on the model of s. typhimurium infection the expressed protective action of bacteria of aerococcus genus was shown; this gives ...19989785800
airways microbial flora in copd patients in stable clinical conditions and during exacerbations: a bronchoscopic investigation.patients affected with chronic obstructive pulmonary disease (copd) undergo frequent exacerbations of their illness characterized by increased cough and expectoration. the precise aetiology of these episodes often remains unknown. in the absence of clinical or radiographic signs of pneumonia, bacterial or viral cultures of sputum usually provide little useful information. thus, we performed fibreoptic bronchoscopy using a protected specimen brush (psb) to obtain uncontaminated secretions for cul ...19989785808
[database on nucleotide sequences used as primers of microorganisms].the data base (db) "primers of microorganisms" for the accumulation and systematization of information on oligonucleotide sequences, used as primers in polymerase chain reaction, has been created. this db includes data on primers for the laboratory diagnostics of 20 bacterial genera (aerococcus, aeromonas, bartonella, borrelia, burkholderia, chlamydia, clostridium, corynebacterium, escherichia, francisella, helicobacter, legionella, listeria, mycobacterium, mycoplasma, salmonella, shigella, stap ...19989825498
root canal microflora: qualitative changes after endodontic instrumentation.the changes in the pattern of bacteria in root canal after repeated washing with naocl and endodontic instrumentation were evaluated. samples were obtained from the root canals of patients before and after endodontic instrumentation, and analyzed according to the usual microbiological techniques. washing with naocl and endodontic instrumentation act against bacteria such as peptostreptococci, streptococcus sanguis, actinomyces israelii, proteus mirabilis, bacteroides, whereas streptococcus lacti ...19892732777
development of bacterial contamination during production of yeast extracts.baker's yeast suspensions having bacterial populations of 10(6) and 10(8) cfu/ml were subjected to autolysis processes designed to obtain yeast extracts (ye). the bacterial contaminants added to the yeast cell suspensions were produced with spent broths obtained from a commercial yeast production plant and contained 59% cocci (leuconostoc, aerococcus, lactococcus) as well as 41% bacilli (bacillus). autolyses were conducted at four different ph levels (4.0, 5.5, 7.0, and 8.5) and with two autolys ...199910388734
septicaemia and endomyocarditis caused by aerococcus urinae.aerococcus urinae, an uncommon urinary tract pathogen, was recently shown to cause septicaemia and endocarditis in a few patients in denmark and the netherlands. in austria this is the first report of a fatal course of endomyocarditis by aerococcus urinae, associated with multiple septic infarcts.199910420497
three cases of serious infection caused by aerococcus urinae.three cases of serious infection caused by aerococcus urinae are presented: a patient with endocarditis and two patients with soft-tissue infection (phlegmon and balanitis respectively). the literature on aerococcus urinae infections is reviewed and the antibiotic therapy discussed. aerococcus urinae is a pathogen isolated primarily from urine specimens of elderly patients with local or systemic predisposing conditions. most infections are mild, but serious infections such as endocarditis and se ...199910421047
aerococcus christensenii sp. nov., from the human vagina.phenotypic and phylogenetic studies were performed on two strains of a hitherto undescribed aerococcus-like organism isolated from the human vagina. comparative 16s rrna gene sequencing studies demonstrated that the unknown strains constitute a new subline within the genus aerococcus. the unknown bacterium was readily distinguished from the two currently recognized aerococcus species, aerococcus viridans and aerococcus urinae, by biochemical tests and electrophoretic analysis of whole-cell prote ...199910425770
[the effect of aerococcus viridans on the properties of salmonellae and staphylococci in vitro].according to electron microscopy the repeated contacts of aerococcus viridans 167 (industrial strain for the probiotic aerobact production) with salmonella typhimurium and staphylococcus aureus caused the deep changes of cellular structure, down to bacteriolysis. remaining viable pathogenic cells gave posterity with changed biochemical properties and virulence. s. typhimurium completely lost typical biochemical properties, its mobility and ability to be agglutinated by o-specific salmonella seru ...199910483228
cloning and analysis of the l-lactate utilization genes from streptococcus iniae.the presence of lactate oxidase was examined in eight streptococcus species and some related species of bacteria. a clone (pgr002) was isolated from a genomic library of streptococcus iniae generated in escherichia coli, containing a dna fragment spanning two genes designated lcto and lctp. we show that these genes are likely to be involved in the l-lactic acid aerobic metabolism of this organism. this dna fragment has been sequenced and characterized. a comparison of the deduced amino acid sequ ...199910508058
studies on antagonism between porcine skin bacteria.strains of staphylococcus warneri, staph. epidermidis and aerococcus viridans isolated from porcine skin were shown to produce substances that inhibited the growth of staph. hyicus. studies of interactions between the inhibitor-producing bacteria and staph. hyicus on cellulose acetate membranes demonstrated a significant reduction (p less than 0.001) of the staph. hyicus population at various stages of its growth cycle, depending on the inhibitor used. inhibitor production profiles in batch cult ...19892666380
identification of aesculin-hydrolyzing streptococci, lactococci, aerococci and enterococci from subclinical intramammary infections in dairy cows.aesculin-hydrolyzing, catalase-negative, gram-positive cocci isolated from subclinical intramammary infections in dairy cows were identified to species level using growth characteristics and biochemical activity. the results indicated that the aesculin-hydrolyzing cocci associated with this type of infection are a very heterogenic group. s. uberis strains, including inulin- or beta-glucuronidase-negative isolates, accounted for only about one-third of the collection, and enterococcus faecalis st ...199910591500
aerococcus viridans infection. case report and review. 19902242644
caries development and aerococcus viridans. 199910613146
on the interpretation of quantitative structure-function activity relationship data for lactate oxidase.the native flavin, fmn, has been removed from the l-lactate oxidase of aerococcus viridans, and the apoprotein reconstituted with 12 fmn derivatives with various substituents at the flavin 6- and 8-positions. impressive linear relationships are exhibited between the sum of the hammett final sigma(para) and final sigma(ortho) parameters and the redox potentials of the free flavins, and between the redox potentials of the free and enzyme-bound flavins. rapid reaction kinetics studies of the recons ...200010706608
[septic arthritis as an initial manifestation of endocarditis].a 60-year-old female patient was admitted to the hospital with high fever and an acute onset of pauciarticular arthritis. clinical examination and ultrasound imaging of the right knee demonstrated a severe synovitis with a large synovial effusion and a baker's cyst. arthrocentesis showed an opaque viscous synovial fluid with a highly elevated white blood cell count. in synovial fluid cultures and in serial blood cultures aerococcus viridans (av) was detected. further examination revealed a mitra ...19902198741
properties and stability of glycerophosphate oxidase isolated from a mutant strain of aerococcus viridans.the properties of microbial l-alpha-glycerophosphate oxidase (gpo) isolated from a mutant strain of aerococcus viridans dbm 1509 were estimated. the stability at different temperatures and ph were detected. at 4 degrees c the enzyme lost activity during 15 d, at 20 degrees c and 30 degrees c gpo activity decreased during 30 and 25 h, respectively. the highest stability was measured at - 20 degrees c and ph 9. at 4 degrees c the stability was enhanced by the addition of 0.1 m edta or by lyophiliz ...200010747248
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