flocculation and adsorption of enzymes during growth of a moderate halophile, micrococcus varians var. halophilus.flocculation of a moderate halophile, micrococcus varians atcc 2197, occurred during growth in complex medium containing 3 m nacl and a concentration of mgso4 and kh2po4 greater than 40 and 14 mm, respectively. extracellular nuclease activity was absent in the flocculated cultures. repeated washing of flocs by mg2+-free tris buffer containing 3 m nacl, lowering of ph value of floc suspension below 6.3, or addition of ethylenediaminetetraacetic acid resulted in complete dissociation of the flocs ...1978307427
the effect of varying sea salt concentration in the growth medium on the chemical composition of a purified membrane fraction from planococcus citreus migula.membrane preparations were prepared from cells of planococcus citreus grown in the presence of three final concentrations of sea salt in a basic growth medium. the concentration of salt in the medium affects the amount of membrane in the cell. the three preparations were subjected to chemical analysis and no significant changes in chemical composition were seen as the salt concentration in the medium was increased. values for the various components generally were within normal ranges and were si ...1977869517
the membrane lipids of planococcus citreus migula from cells grown in the presence of three different concentrations of sea salt added to a basic medium. 1977869518
simple extraction and bioassay of the female sex pheromone of the citrus mealybug, planococcus citri. 19761252040
phylogenetic analysis of the genera planococcus, marinococcus and sporosarcina and their relationships to members of the genus bacillus.a phylogenetic analysis based on 16s rrna was performed on the genera planococcus, marinococcus, sporosarcina and endospore-forming rods. in agreement with earlier 16s rrna cataloguing data, planococcus citreus and sporosarcina ureae clustered with bacillus pasteurii and other bacilli containing lysine in their cell walls. sporosarcina halophila was shown to be genetically distinct from s. ureae and formed a loose association with the main bacillus subtilis grouping. marinococcus halophilus (for ...19921505740
cloning and expression of drosophila hp1 homologs from a mealybug, planococcus citri.the mealybug chromosome cycle is one of the most dramatic examples of genomic imprinting known. in embryos that are to become male the entire paternal chromosome set becomes heterochromatic and inactive at the blastoderm stage, while the maternal set remains active and euchromatic. hp1 is a protein from drosophila melanogaster, which binds preferentially to heterochromatin on polytene chromosomes and is likely to be a modifier of position effect variegation. this paper describes the isolation an ...19921629256
role of various microorganisms on tc behavior in sediments.marine bacteria (moraxella sp., planococcus sp. and a mixed population of anaerobes) from a coastal sediment were found to concentrate tc. maximum concentration of this element occurred during the stationary phase of growth of the bacteria, at low redox potential. a metabolic process seems responsible for tc concentration by bacteria, in which it binds to high molecular weight cellular constituents. polysaccharidic polymers, which were visualized around the bacterial cells with the scanning elec ...19892592213
nucleotide sequences of 5s ribosomal rnas of planococcus citreus, planococcus kocurii and sporosarcina ureae. 19883194204
rapid lysostaphin test to differentiate staphylococcus and micrococcus species.a rapid, simple lysostaphin lysis susceptibility test to differentiate the genera staphylococcus and micrococcus was evaluated. of 181 strains from culture collections, 95 of 95 staphylococcus strains were lysed, and 79 of 79 micrococcus strains were not lysed. the seven planococcus strains were resistant. clinical isolates (890) were tested with lysostaphin and for the ability to produce acid from glycerol in the presence of erythromycin. overall agreement between the methods was 99.2%. all cli ...19863519667
distribution and types of bacteria isolated from an inland saltern.the physico-chemical characteristics and bacterial populations of an inland saltern were studied. the well water leading into the ponds of the saltern differed from sea water in its total salinity (18% (w/v)) and had lower mg2+ and so4(2-) contents and much higher contents of cl-, ca2+- and k+. a high proportion of halotolerant microorganisms were isolated, although marine bacteria were not found. most of the halophilic strains isolated appeared to be typical moderate halophiles, and included si ...19873606874
effects of temperature and sodium chloride concentration on the phospholipid and fatty acid compositions of a halotolerant planococcus sp.the phospholipid headgroup composition and fatty acid composition of a gram-positive halotolerant planococcus sp. (strain a4a) were examined as a function of growth temperature (5 to 35 degrees c) and nacl content (0 to 1.5 m) of the growth medium. when the growth temperature was decreased, the relative amount of mono-unsaturated branched-chain fatty acids increased. when planococcus sp. strain a4a was grown in media containing high nacl concentrations, the relative amount of the major fatty aci ...19853980436
differential dna synthesis in heterochromatic and euchromatic chromosome sets of planococcus citri. 19704104058
purification and characterization of serologically active cell wall substances from planococcus strains. 19734134798
amino acid sequence of the murein of planococcus and other micrococcaceae.the amino acid composition and amino acid sequence of the murein (peptidoglycan) of 10 strains of planococci were studied. it is shown that the peptide subunit consists of muramyl-l-alanyl-gamma-d-glutamyl-l-lysyl-d -alanine. the cross-linking of two adjacent peptide subunits is mediated by d-glutamic acid which is bound to the epsilon-amino group of lysine by its gamma-carboxyl group and to the carboxyl group of d-alanine of an adjacent peptide subunit by its amino group. about 20 to 25% of the ...19704247024
[studies on the filter-chamber of planococcus citri risso (insecta, homoptera). histochemistry and ultrastructure (author's transl)]. 19734359268
endomitosis in the mealy bug, planococcus citri (homoptera: coccoidea). 19685722739
[planococcus isolated from the water of the mediterranean]. 19655865412
correlation between phosphorylated h1 histone and condensed chromatin in planococcus citri.histones of the mealybug planococcus citri have been isolated and characterised. although no major differences were observed between the core histones of male and female insects, the pattern of h1 histones was significantly different between the sexes. a specific hyperphosphorylated h1 was found to be present only in male mealybugs, where the paternal chromosomes are condensed and transcriptionally inactive.19836628682
numerical taxonomy of staphylococci.over two hundred staphylococci from human and animal sources and representatives of established species of staphylococcus, micrococcus and planococcus were compared in a numerical phenetic survey using 115 unit characters. data were analyzed using the jaccard coefficient and the unweighted pair group method with averages algorithm. cluster composition was not markedly affected by test error, estimated as 3.49%. the staphylococci were assigned to eighteen clusters containing four or more strains ...19836659744
variation in pigment production by planococcus citreus migula with cultural age and with sea salt concentration in the medium.the biosynthesis of carotenoids and the type of carotenoids produced by planococcus citreus migula appear to be influenced by the concentration of sea salt in the medium and by the age of culture. quantitatively, pigmentation peaks at a time corresponding to the presence of maximum cell numbers and then undergoes a rapid decline.19807396481
possible involvement of planococcus halophilus in an outbreak of necrotic hepatitis in chickens. 19957725615
phylogenetic interrelationships of round-spore-forming bacilli containing cell walls based on lysine and the non-spore-forming genera caryophanon, exiguobacterium, kurthia, and planococcus.the 16s rrna gene sequences of "bacillus aminovorans" and several species considered to be phylogenetically related to the group 2 bacilli of ash et al. (c. ash, j. a. e. farrow, s. wallbanks, and m. d. collins, lett. appl. microbiol. 13:202-206, 1991) were determined. a comparative analysis of the sequence data revealed that the round-spore-forming group 2 bacilli, together with some asporogenous taxa (the genera caryophanon, exiguobacterium, kurthia, planococcus), form a phylogenetically disti ...19948123563
a male-specific nuclease-resistant chromatin fraction in the mealybug planococcus mealybugs, chromatin condensation is related to both genomic imprinting and sex determination. the paternal chromosomal complement is condensed and genetically inactive in sons but not in daughters. during a study of chromatin organization in planococcus lilacinus, digestion with micrococcal nuclease showed that 3% to 5% of the male genome is resistant to the enzyme. this nuclease resistant chromatin (nrc) apparently has a nucleosomal organization. southern hybridization of genomic dna sugges ...19968575251
characterization of a salt-tolerant family 42 beta-galactosidase from a psychrophilic antarctic planococcus isolate.we isolated a gram-positive, halotolerant psychrophile from a hypersaline pond located on the mcmurdo ice shelf in antarctica. a phylogenetic analysis of the 16s rrna gene sequence of this organism showed that it is a member of the genus planococcus. this assignment is consistent with the morphology and physiological characteristics of the organism. a gene encoding a beta-galactosidase in this isolate was cloned in an escherichia coli host. sequence analysis of this gene placed it in glycosidase ...200010831422
[the use of batumin-containing disks for the rapid identification of staphylococci].the study of 467 microbial strains obtained from collections and from clinical sources revealed that microorganisms of the genus staphylococcus were highly sensitive to batumin, a new antibiotic obtained from bacteria of the genus pseudomonas. 378 strains of 15 staphylococcus species proved to be highly sensitive to the diagnostic preparation "diastaph", developed on the basis of batumin (antibiotic-impregnated discs); after 18-hour incubation the diameter of the growth inhibition zones on agar- ...199910852028
isolation and characterization of a novel hydrocarbon-degrading, gram-positive bacterium, isolated from intertidal beach sediment, and description of planococcus alkanoclasticus sp. nov.characterization of a bacterial isolate (strain mae2) from intertidal beach sediment capable of degrading linear and branched alkanes.200111168727
involvement of histone h4 acetylation in the epigenetic inheritance of different activity states of maternally and paternally derived genomes in the mealybug planococcus citri.modification of histones by acetylation is a well-known mechanism for the establishment and maintenance of specific chromatin structures with different activity states. in planococcus citri males the paternal genome, early in development, becomes mostly inactive and heterochromatic. as we had not found methylation in the genome of p. citri, we analyzed the acetylation state of histone h4. we report here that, in males, differences in the level of histone h4 acetylation are indeed present in the ...200111453559
planomicrobium koreense gen. nov., sp. nov., a bacterium isolated from the korean traditional fermented seafood jeotgal, and transfer of planococcus okeanokoites (nakagawa et al. 1996) and planococcus mcmeekinii (junge et al. 1998) to the genus planomicrobium.a bacterial strain, jg07t, isolated from the korean traditional fermented seafood jeotgal, was subjected to a polyphasic taxonomic study. cells of strain jg07t are cocci or short rods in the early growth phase but change to rods as the cultures age. the peptidoglycan type is a4alpha, based on l-lys-d-glu. the menaquinone profile is characterized by the predominance of mk-8 followed by mk-7 and mk-6. the cellular fatty acid profile contains major amounts of saturated, unsaturated and branched fat ...200111491353
molecular microbial diversity of a spacecraft assembly ongoing investigations to map and archive the microbial footprints in various components of the spacecraft and its accessories, we have examined the microbial populations of the jet propulsion laboratory's spacecraft assembly facility (jpl-saf). witness plates made up of spacecraft materials, some painted with spacecraft qualified paints, were exposed for approximately 7 to 9 months at jpl-saf and examined the particulate materials collected for the incidence of total cultivable aerobic heter ...200111518337
genetic characterization of a putative densovirus from the mealybug planococcus genomic dna preparations from the citrus mealybug, planococcus citri, contained a dna band corresponding to 5.5 kilobases. this dna was a linear molecule and was cloned into puc18. nucleotide sequence determination indicated that it was the replicative form of a densovirus, most closely related to the virus from periplaneta fuliginosa (smokybrown cockroach).200111685517
characterization of a halotolerant-psychroloterant bacterium from dry valley antarctic soil.the saline soils of the ice free dry valleys of victoria land, antarctica may provide the closest analog on earth to martian conditions. we have initiated a study aimed at examining microbial adaptations to the harsh environment of these dry valley soils. in this report we describe the characterization of one bacterium, strain a4a, isolated from taylor valley soil. strain a4a was an obligately aerobic, orange-pigmented, gram-positive coccus that grew over wide ranges of both temperature (0 degre ...198311542754
influence of egg load and oviposition time interval on the host discrimination and offspring survival of anagyrus pseudococci (hymenoptera: encyrtidae), a solitary endoparasitoid of citrus mealybug, ++planococcus citri (hemiptera: pseudococcidae).oviposition and host discrimination behaviour of unmated anagyrus pseudococci (girault), an endoparasitoid of the citrus mealybug planococcus citri (risso), were investigated in the laboratory. female parasitoids were able to discriminate between parasitized hosts and healthy ones. the mean number of ovipositions was significantly higher in unparasitized than in parasitized hosts. conspecific-superparasitism occurred more often than self-superparasitism. changes in consecutive ovipositions over ...200010948366
hot water treatment and insecticidal coatings for disinfesting limes of mealybugs (homoptera: pseudococcidae).hot water immersion and insecticidal coatings were tested to determine if they could be used to disinfest persian limes, citrus latifolia tanaka, of the mealybug pests planococcus citri risso and pseudococcus odermatti miller & williams. a 20-min 49 degrees c hot water immersion treatment is effective in killing mealybugs and all other arthropods tested found externally on limes, or under the calyx. no insects or mites were found to survive after the 20-min hot water treatment. in this test, 7,2 ...200010902365
sex-specific organisation of middle repetitive dna sequences in the mealybug planococcus lilacinus.differential organisation of homologous chromosomes is related to both sex determination and genomic imprinting in coccid insects, the mealybugs. we report here the identification of two middle repetitive sequences that are differentially organised between the two sexes and also within the same diploid nucleus. these two sequences form a part of the male-specific nuclease-resistant chromatin (nrc) fraction of a mealybug planococcus lilacinus. to understand the phenomenon of differential organisa ...199910471745
pathogenic effects of bacteria isolated from larvae of hylesia metabus crammer (lepidoptera: saturniidae).hylesia metabus larvae are susceptible to several pathogens indigenous to the area in which they are found. some larvae show symptoms characteristic of bacterial infection; they become flaccid and lethargic, and show a marked loss of appetite. we isolated and identified 29 bacterial strains from live, dead and experimentally infected h. metabus larvae, and evaluated their pathogenic activity. the bacteria which caused mortality in the larvae were: pseudomonas aeruginosa (60-93.3%), proteus vulga ...200212234536
natural enemies of three mealybug species (hemiptera: pseudococcidae) found on citrus and effects of some insecticides on the mealybug parasitoid coccidoxenoides peregrinus (hymenoptera: encyrtidae) in south africa.the population density of mealybug species in some south african citrus orchards has increased to pest status in recent years. the characterization of the natural enemy complex and quantification of their contribution to the control of planococcus citri (risso), pseudococcus longispinus (targioni-tozzetti) and pseudococcus calceolariae (maskell) on citrus limon (l.) and citrus reticulata (blanco) was investigated through intensive sampling. eight primary and four secondary parasitoids, and two p ...200312762866
cytosine methylation is not involved in the heterochromatization of the paternal genome of mealybug planococcus citri. 199910219735
triple coitus in the mealy bug, planococcus citri (risso). 195913632773
a study of sex predetermination in the mealy bug planococcus citri (risso). 196013728321
the relationship between dna methylation and chromosome imprinting in the coccid planococcus citri.the phenomenon of chromosome, or genomic, imprinting indicates the relevance of parental origin in determining functional differences between alleles, homologous chromosomes, or haploid sets. in mealybug males (homoptera, coccoidea), the haploid set of paternal origin undergoes heterochromatization at midcleavage and remains so in most of the tissues. this different behavior of the two haploid sets, which depends on their parental origin, represents one of the most striking examples of chromosom ...199910101170
the effects of radiation damaged heterochromatic chromosomes on male fertility in the mealy bug, planococcus citri (risso). 196214478958
novel polar lipids of halophilic eubacterium planococcus h8 and archaeon haloferax part of a study to identify novel lipids with immune adjuvant activity, a structural comparison was made between the polar lipids from two halophiles, an archaeon haloferax volcanii and a eubacterium planococcus h8. h. volcanii polar lipid extracts consisted of 44% archaetidylglycerol methylphosphate, 35% archaetidylglycerol, 4.7% of archaeal cardiolipin, 2.5% archaetidic acid, and 14% sulfated glycolipids 1 and 2. nuclear magnetic resonance (nmr) and fast atom bombardment mass spectrometry ( ...200314499737
the enzymatic activity of phosphoglycerate mutase from gram-positive endospore-forming bacteria requires mn2+ and is ph sensitive.the enzymatic activity of phosphoglycerate mutase (pgm) from three gram-positive endospore-forming bacteria (bacillus subtilis, clostridium perfringens, and sporosarcina ureae) requires mn2+ and is very sensitive to ph; at low concentrations of mn2+, a ph change from 8 to 6 resulted in greater than 30- to 200-fold decreases in the activity of these pgms. however, pgm deactivation at ph 6 was reversed by shifting the enzyme to ph 7 or 8. free mn2+ was not directly involved in pgm catalysis, altho ...19989830105
arthrobacter, brachybacterium and planococcus isolates identified from antarctic sea ice brine. description of planococcus mcmeekinii, sp. nov.three facultative psychrophilic gram-positive bacterial strains were isolated from brine samples from the sea ice community in antarctica. all strains were coccoid to rod-shaped and exhibited broad salinity and temperature ranges for growth. the three strains were subjected to 16s rdna sequencing and subsequent phylogenetic analysis. all possess unique 16s rdna sequences indicating they are new, previously unreported organisms. phylogenetic analyses coupled with phenotypic characterization indic ...19989704115
psychrophilic planococcus maitriensis sp.nov. from orange pigmented bacterium, s1, was isolated from a cyanobacterial mat sample collected in the vicinity of schirmacher oasis, maitri, the indian station, in antarctica. the bacterium is gram-positive and possesses all the characteristics of the genus planococcus. it is non-sporulating, motile and has a4alpha type peptidoglycan, mk-7 and mk-8 as the major menaquinones and anteiso-c(15:0) as the major fatty acid. based on the phylogenetic characteristics, the bacterium s1 is identified as a clo ...200314666977
partial purification of antibacterial proteinaceous factors from erythrocytes of oncorhynchus mykiss.antimicrobial peptides are natural antibiotics known to be present in both myeloid cells and epithelial surfaces of vertebrates. nevertheless, the reports of antimicrobial peptides isolated from blood cells of teleosts are scarce. in this paper we show that acid-soluble erythrocyte extracts from rainbow trout, oncorhynchus mykiss, display antibacterial activity against planococcus citreus on a radial diffusion assay. following tc18 solid phase extraction, cationic exchange chromatography and c18 ...200414675829
identification of a sex pheromone component of pseudococcus cryptus.a sex pheromone component of pseudococcus cryptus has been isolated and identified. the crude pheromone extract obtained by airborne collection was fractionated by liquid chromatography (lc) on florisil, and further purified by high performance liquid chromatography and preparative gas chromatography (gc). the pheromone component was shown to be an ester, the alcohol part of which was identical to the known alcohol moiety of the pheromone of planococcus citri. the chemical structure was determin ...200314682507
styelins, broad-spectrum antimicrobial peptides from the solitary tunicate, styela clava.two novel phenylalanine-rich antimicrobial peptides, styelin a and styelin b, were purified from the hemocytes of styela clava. the peptides had very similar masses (styelin a, 3685.8; styelin b, 3700.6) and amino acid compositions, and at least 17 of their first 20 n-terminal residues were identical. both styelins were effective against a panel of gram negative and gram positive bacterial pathogens of humans, usually acting with minimal inhibitory concentrations < 1.5 microgram/ml (< 0.5 microm ...19979467865
[sensitization and allergic pathology in a group of workers employed in insect breeding for biological pest control].on the basis of the results of a previous study and immuno-allergological tests, a group of workers employed in breeding insects for biological pest control were studied to ascertain whether any new sensitizations to arthropods recently included in the work cycle occurred and whether any new allergic diseases were observed. the results confirmed a high sensitization to ephestia kuehniella and to orius, the latter probably due to contamination; sensitization to tetranicus urticae and to planococc ...19979379990
identification and nh2-terminal amino acid sequences of dnak and groel homologues in moderate eubacterial halophiles.we have identified 2 dnak and 3 groel homologues from moderately halophilic acinetobacter, pseudomonas, and planococcus species by partial purification using an atp-agarose column and by the analysis and similarity search of these nh2-terminal amino acid sequences. although these bacteria required 1 to 2m nacl for growth, these dnak and groel homologues did not require high salt to bind to the atp column, thus suggesting that these chaperones did not require high salts for their biochemically ac ...19979301123
coffea arabica l., a new host plant for acetobacter diazotrophicus, and isolation of other nitrogen-fixing acetobacteria.acetobacter diazotrophicus was isolated from coffee plant tissues and from rhizosphere soils. isolation frequencies ranged from 15 to 40% and were dependent on soil ph. attempts to isolate this bacterial species from coffee fruit, from inside vesicular-arbuscular mycorrhizal fungi spores, or from mealybugs (planococcus citri) associated with coffee plants were not successful. other acid-producing diazotrophic bacteria were recovered with frequencies of 20% from the coffee rhizosphere. these n2-f ...19979293018
diversity and association of psychrophilic bacteria in antarctic sea ice.the bacterial populations associated with sea ice sampled from antarctic coastal areas were investigated by use of a phenotypic approach and a phylogenetic approach based on genes encoding 16s rrna (16s rdna). the diversity of bacteria associated with sea ice was also compared with the bacterial diversity of seawater underlying sea ice. psychrophilic (optimal growth temperature, < or = 15 degrees c; no growth occurring at 20 degrees c) bacterial diversity was found to be significantly enriched i ...19979251193
developmental analysis of a female-specific 16s rrna gene from mycetome-associated endosymbionts of a mealybug, planococcus lilacinus.a clone showing female-specific expression was identified from an embryonic cdna library of a mealybug, planococcus lilacinus. in southern blots this clone (p7) showed hybridization to genomic dna of females, but not to that of males. however, p7 showed no hybridization to nuclei of either sex, raising the possibility that it was extrachromosomal in origin. in sectioned adult females p7 hybridized to an abdominal organ called the mycetome. the mycetome is formed by mycetocytes, which are polyplo ...19969035384
emendation of the genus planococcus and transfer of flavobacterium okeanokoites zobell and upham 1944 to the genus planococcus as planococcus okeanokoites comb. nov.the taxonomic position of flavobacterium okeanokoites ifo 12536t (t = type strain) was determined by 16s rrna gene sequencing and chemotaxonomic methods. phylogenetic evidence derived from a 16s rrna sequence analysis indicated that f. okeanokoites, which forms rod-shaped cells, belongs to the genus planococcus, which forms spherical cells. a phylogenetically close relationship was supported by chemotaxonomic characteristics, such as the presence of menaquinone 7 and menaquinone 8 as major isopr ...19968863410
effects of insect growth regulators on citrus mealybug parasitoid leptomastix dactylopii (hymenoptera: encyrtidae).in this study, we measured the effects of three commonly used insecticides classified as insect growth regulators, on the encyrtid parasitoid leptomastix dactylopii (howard) when parasitizing citrus mealybug, planococcus citri (risso). kinoprene, pyriproxyfen, and azadirachtin were evaluated in both petri dish and a cage experiment at label-recommended rates to measure their effects on the mortality, parasitization rate, and sex ratio of l. dactylopii. insecticides were applied to petri dishes a ...200415384332
comparison of antibacterial activity in the hemocytes of different crustacean species.antibacterial activity in hemocytes of the squat lobster, galathea strigosa, the norway lobster, nephrops norvegicus, the common shrimp, crangon crangon, and the giant antarctic isopod, glyptonotus antarcticus, was investigated in vitro. for all species, the marine bacterium, psychrobacter immobilis, was used as the test organism, although with g. antarcticus, the gram positive bacteria, planococcus citreus and bs 68 (an isolate from antarctic waters), were also used. hemocyte lysate supernatant ...19957866773
effects of monovalent and divalent salts on the phospholipid and fatty acid compositions of a halotolerant planococcus sp.the phospholipid headgroup and fatty acid compositions of a halotolerant planococcus sp. (strain a4a) were examined when cells were grown in the presence of high concentrations of a variety of salts. the fatty acid composition of planococcus sp. strain a4a was altered primarily as a function of the osmolality of the growth medium. the phospholipid headgroup composition was influenced by both the osmolality of the growth medium and the nature of the cation species present. an increase in the card ...19863801094
planomicrobium chinense sp. nov., isolated from coastal sediment, and transfer of planococcus psychrophilus and planococcus alkanoclasticus to planomicrobium as planomicrobium psychrophilum comb. nov. and planomicrobium alkanoclasticum comb. aerobic, motile, gram-positive strain, dx3-12(t), was isolated from coastal sediment of the eastern china sea in fujian province, china, and was identified by means of polyphasic taxonomy. on the basis of 16s rrna gene sequence similarity, strain dx3-12(t) was closely related to members of the genera planomicrobium and planococcus. 16s rrna gene sequence similarities between strain dx3-12(t) and planomicrobium mcmeekinii, planomicrobium koreense, planomicrobium okeanokoites, planococcus psych ...200515774646
[demonstration of glycocalyx formation in vitro in citrobacter freundii, proteus mirabilis and planococcus sp.: the effect of polyosidases].three bacterial species of different origin, citrobacter freundii, proteus mirabilis and planococcus sp., formed glycocalyx in vitro and thereby showed that the phenomena does not only occur in vivo contrary to the opinion of many authors. the glycocalyx was produced in the attachment processes used or both extra- and intercellular attachment. the aggregates formed caused large errors in bacterial counts and led to questioning of the very principle of the counting procedures. the glycocalyx was ...19873135828
comparative 16s rrna oligonucleotide analyses and murein types of round-spore-forming bacilli and non-spore-forming relatives.the phylogenetic incoherency of the genus bacillus as presently described is demonstrated by analysis of both published and new data from comparative 16s rrna cataloguing of nine bacillus species and a number of related non-bacillus taxa, i.e. caryophanon latum, filibacter limicola and planococcus citreus. while the ellipsoidal-spore-forming bacilli, e.g. b. subtilis and allied species, formed a coherent cluster, the round-spore-forming bacilli showed a higher degree of relationship to the non-s ...19872452227
16s ribosomal rna analysis of filibacter limicola indicates a close relationship to the genus bacillus.the phylogenetic relationship of the gram-negative, filamentous gliding bacterium filibacter limicola was analysed by 16s rrna oligonucleotide cataloguing. in contrast to the proposed membership of this asporogenous species in the flexibacteriaceae, filibacter limicola clusters phylogenetically with the gram-positive eubacteria bacillus pasteurii, sporosarcina ureae and the asporogenous species planococcus citreus. the genetic relationship is supported by several common phenotypic properties.19852415672
[characterization of communities of heterotrophic bacteria associated with healthy and diseased corals in nha trang bay (vietnam)].a comparative investigation of the heterotrophic microflora of 11 species of healthy corals and of white-band-diseased and yellow-band-diseased corals inhabiting the reefs of nha trang bay (vietnam), which has been exposed to anthropogenic impact, was performed. fifty-nine strains of heterotrophic bacteria isolated on y/k and endo media were investigated and characterized. all the isolates were identified at the genus level by consideration of the results of analysis of their phenotypic properti ...200516315986
effects of monovalent and divalent salts on the phospholipid and fatty acid compositions of a halotolerant planococcus sp.[this corrects the article on p. 581 in vol. 52.].198616347252
detection of a cpa methylase in an insect system: characterization and substrate specificity.a cytosine-specific dna methyltransferase (ec has been purified to near homogeneity from a mealybug (planococcus lilacinus). the enzyme can methylate cytosine residues in cpg sequences as well as cpa sequences. the apparent molecular weight of the enzyme was estimated as 135,000 daltons by fplc. the enzyme exhibits a processive mode of action and a salt dependence similar to mammalian methylases. mealybug methylase exhibits a preference for denatured dna substrates.19921374836
numerical taxonomy and ecology of oligotrophic bacteria isolated from the estuarine environment.slow-growing bacteria, isolated on nutrient-rich and nutrient-limited media, from chesapeake bay water and sediment samples, were examined for 119 biochemical, cultural, morphological, nutritional, and physiological characters. those bacteria which grow on low nutrient media, termed oligotrophs, a total of 162 strains, were subjected to taxonomic analysis, as a preliminary step in determining their ecological significance. the data for all strains included in the study were examined by computer ...1977871972
the relevance of bacterial lytic activity in the taxonomy of the micrococcaceae: failure of its production by micrococcus and planococcus as opposed to staphylococcus. 1978745006
planococcus antarcticus and planococcus psychrophilus spp. nov. isolated from cyanobacterial mat samples collected from ponds in antarctica.thirteen orange-pigmented bacteria associated with cyanobacterial mat samples collected from four different lakes in mcmurdo, antarctica, were isolated. twelve of the isolates, which were coccoid in shape, were very similar and possessed all the characteristics of the genus planococcus and represented a new species, which was assigned the name planococcus antarcticus sp. nov. (cms 26or(t)). apart from the phenotypic differences, p. antarcticus differed from all reported species of planococcus by ...200212072961
facultative heterochromatization in parahaploid male mealybugs: involvement of a heterochromatin-associated protein.the behavior of chromosomes during development of the mealybug planococcus citri provides one of the most dramatic examples of facultative heterochromatization. in male embryos, the entire haploid paternal chromosome set becomes heterochromatic at mid-cleavage. male mealybugs are thus functionally haploid, owing to heterochromatization (parahaploidy). to understand the mechanisms underlying facultative heterochromatization in male mealybugs, we have investigated the possible involvement of an hp ...200111585806
characterization of the genome of the mealybug planococcus lilacinus, a model organism for studying whole-chromosome imprinting and inactivation.the co-occurrence of three chromosome-wide phenomena--imprinting, facultative heterochromatization and diffuse centromere--in the mealybug planococcus lilacinus makes investigation of the genomics of this species an attractive prospect. in order to estimate the complexity of the genome of this species, 300 random stretches of its dna, constituting approximately 0.1% of the genome, were sequenced. coding sequences appear to constitute approximately 53.5%, repeat sequences approximately 44.5% and ...200212073549
a novel antimicrobial function for a ribosomal peptide from rainbow trout antimicrobial peptide was purified from skin secretions and epithelial cells of rainbow trout by cation exchange and reversed phase chromatography. partial n-terminal amino acid sequence of the purified peptide revealed 100% identity with the first 11 residues of a 40s ribosomal peptide from medaka fish. its molecular mass, determined by matrix-associated laser desorption/ionisation mass spectrometry, was found to be 6676.6da. these results indicate that this antimicrobial peptide is likely t ...200212147245
development and optimization of methods for using sex pheromone for monitoring the mealybug planococcus ficus (homoptera: pseudococcidae) in california vineyards.the sex pheromone of the vine mealybug planococcus ficus signoret has been identified as a single component, lavandulyl senecioate. racemic lavandulyl senecioate was as attractive to male mealybugs as the insect-produced (s)-enantiomer, indicating that the unnatural enantiomer is not inhibitory. lavandulol, which also was found in extracts from virgin females, antagonized attraction of males at higher doses. rubber septum lures loaded with 10- to 1,000-microg doses of the pheromone were equally ...200212216810
sex pheromone of the vine mealybug, planococcus ficus in israel: occurrence of a second component in a mass-reared population.two pheromonal components were detected in airborne collections from the vine mealybug planococcus ficus (signoret) (hemiptera: pseudococcidae) mass-reared on potato sprouts. the compounds were identified as (s)-lavandulyl senecioate (i) and (s)-lavandulyl isovalerate (ii) by gc and gc-ms by comparison with synthetic standards. chiral gc analysis on a cyclodextrin column established their chirality. compound i was identified recently as the sex pheromone of p. ficus in california. the attraction ...200312775156
inverse meiosis in triploid females of the mealy bug, planococcus citri. 196214020020
planococcus rifietensis sp. nov, isolated from algal mat collected from a sulfurous spring in campania (italy).the taxomony of strain m8, isolated from algal mat formed at the origin of a sulfurous spring in rifieto (savignano irpino, campania, italy), was investigated in a polyphasic approach. the morphological, physiological and genetic characteristics were compared with of planococcus and planomicrobium species. the isolate grew optimally at ph 9.0, 1.8 m nacl at 37 degrees c. the cells were gram-positive cocci that form pairs, tetrads and aggregates of several cells. the isolate was aerobic/microaero ...200314529178
molecular cloning and characterization of the gene encoding cold-active beta-galactosidase from a psychrotrophic and halotolerant planococcus sp. l4.the gene bgap encoding cold-active beta-galactosidase from a psychrotrophic and halotolerant planococcus sp. l4 was cloned into escherichia coli and sequenced. a sequence analysis of the dna responsible for the bgap gene revealed an open reading frame of 2031 bp encoding for a protein of 677 amino acid residues. the bgap was heterologously expressed in e. coli and purified followed by ni2+ affinity chromatography. the molecular mass of the native enzyme was approximately 156 kda as determined by ...200717326654
planococcus maritimus sp. nov., isolated from sea water of a tidal flat in korea.two gram-positive, motile cocci, strains tf-9(t) and tf-16, were isolated from sea water of a tidal flat in korea. phylogenetic analysis based on 16s rdna sequences showed that the strains fall within the radiation of the cluster comprising planococcus citreus and planococcus kocurii, and this cluster joined the clade comprising planomicrobium species and planococcus alkanoclasticus and planococcus psychrophilus with a high bootstrap resampling value. strains tf-9(t) and tf-16 contained mk-8, mk ...200314657138
dna methylation in insects.cytosine dna methylation has been demonstrated in numerous eukaryotic organisms and has been shown to play an important role in human disease. the function of dna methylation has been studied extensively in vertebrates, but establishing its primary role has proved difficult and controversial. analysing methylation in insects has indicated an apparent functional diversity that seems to argue against a strict functional conservation. to investigate this hypothesis, we here assess the data reported ...200415056357
isolation and characterisation of new planococcus sp. strain able for aromatic hydrocarbons planococcus sp. strain s5 able to grow on salicylate or benzoate as sole carbon source was isolated from activated sludge adapted to sodium salicylate degradation. s5 was determined to be a strictly aerobic, gram-positive, catalase positive, oxidase negative, non-motile, non-spore forming coccus. the strain harboured a plasmid, named pls5. the s5 strain when grown on salicylate expressed both catechol 1,2-dioxygenase and catechol 2,3-dioxygenase activities and degraded this substrate by both ...200315095927
synthesis of the female sex pheromone of the citrus mealybug, planococcus citri.the citrus mealybug, planococcus citri (risso) is a common pest in the southern u. s. and the mediterranean. two alternative syntheses of the female sex pheromone, (1r)-(+)-cis-2,2-dimethyl-3-isopropenyl-cyclobutane methanol acetate, have been developed. key transformations include an allylic oxidation of (1r)-(+)-alpha-pinene to (+)-r-verbenone, oxidative decarboxylation using rucl(3)-naio(4), and methylenation with zn/ch(2)br(2)/ticl(4).200415137832
two novel muramidases from skin mucosa of rainbow trout (oncorhynchus mykiss).two novel antibacterial muramidases were purified to homogeneity from skin exudates of rainbow trout (oncorhynchus mykiss). unusually, one has an acidic isoelectric point and it is the first anionic muramidase to be reported for fish. its molecular mass is 14,268 da, as determined by mass spectrometry. the other muramidase is cationic with a mass of 14,252 da. partial n-terminal amino acid sequencing and peptide mapping strongly point to it being a c-type lysozyme, the first to be purified and c ...200415142536
liposome adjuvants prepared from the total polar lipids of haloferax volcanii, planococcus spp. and bacillus firmus differ in ability to elicit and sustain immune responses.immune stimulating activity was compared for lipid vesicles consisting of the total polar lipids of an archaeon haloferax volcanii, and the eubacteria planococcus spp. and bacillus firmus. each total polar lipid extract readily formed liposomes of similar size, within which the protein antigen ovalbumin was entrapped, with comparable loading and internalization. subcutaneous immunization of mice resulted in anti-ovalbumin antibody titers for all adjuvants, with memory recall responses that were ...200415149772
sex pheromone of the citrus mealybug planococcus citri: synthesis and optimization of trap parameters.a simple synthesis of the pheromone of the citrus mealybug, planococcus citri (risso) (hemiptera: pseudococcidae), has been developed. various factors affecting capture of males have been assessed to optimize the trap design and to develop a lure with high efficacy and longevity. male capture was the same with the racemic and chiral pheromone; technical pheromone (85% purity) was statistically as attractive as pure pheromone (97%). a special formulation was used to determine the actual release r ...200415154456
apolipoproteins a-i and a-ii are potentially important effectors of innate immunity in the teleost fish cyprinus carpio.we have previously shown that high density lipoprotein is the most abundant protein in the carp plasma and displays bactericidal activity in vitro. therefore the aim of this study was to analyze the contribution of its principal apolipoproteins, apoa-i and apoa-ii, in defense. both apolipoproteins were isolated by a two step procedure involving affinity and gel filtration chromatography and were shown to display bactericidal and/or bacteriostatic activity in the micromolar range against gram-pos ...200415233794
germline cyst development and imprinting in male mealybug planococcus the epigenetic modifications involved in the phenomenon of imprinting, which is thought to take place during gametogenesis, one of the primary roles is exerted by histone tail modifications acting on chromatin structure. what is more, in insects like mealybugs, with a lecanoid chromosome system, imprinting is strictly related to sex determination. in many diverse species gametes originate in specific, highly evolutionarily conserved structures called germline cysts. the use of staining techni ...200415503092
planococcus stackebrandtii sp. nov., isolated from a cold desert of the himalayas, india.the taxonomic position of a bacterium isolated from a cold desert of the himalayas, india, was analysed by using a polyphasic approach. the isolated strain, designated k22-03t, had phenotypic characteristics that matched those of the genus planococcus and it represents a novel species. the almost-complete 16s rrna gene sequence (1464 bases) of the novel strain was compared with those of previously studied planococcus type strains and confirmed that the strain belongs to the genus planococcus. 16 ...200515653859
characterisation of banana streak mysore virus and evidence that its dna is integrated in the b genome of cultivated musa.we have sequenced the complete genome of an isolate of banana streak virus from banana cv. 'mysore' and show that it is sufficiently different from a previously characterised isolate from cv. 'obino l'ewai' to warrant recognition as a distinct species, for which the name banana streak mysore virus (bsmysv) is proposed. the structure of the bsmysv genome was typical of badnaviruses in general, although orf i had a non-conventional start codon. evidence that at least part of the bsmysv genome is i ...200515785970
diversity of reed (phragmites australis) stem biofilm bacterial communities in two hungarian soda lakes.from reed biofilm samples of kelemen-szék (kiskunság national park, knp) and nagy-vadas (hortobágy national park, hnp) altogether 260 bacterial isolates were gained after serial dilutions and plating onto different media. following a primary selection 164 strains were investigated by "traditional" phenotypic tests and clustered by numerical analysis. fifty-six representative strains were selected to ardra and 16s rdna sequence analysis for identification. strains were identified as members of ge ...200718088008
comparison of mealybug (planococcus lilacinus) and fruit fly genomes: isolation and analysis of conserved sequences and their utility in studying synteny in the using ligation-mediated pcr products from mealybug dna as tester and biotinylated fly dna as driver, we recovered a fraction of the tester that remains hybridized to driver following high-stringency washing conditions. this fraction is expected to contain mealybug sequences conserved in the fly (mcf). reciprocal experiments enabled the isolation of fly sequences conserved in the mealybug (fcm). coding sequences among mcf show amino acid identities >40% with fly proteins, allowing a reliable i ...200818253039
hot-water treatments for control of planococcus ficus (homoptera: pseudococcidae) on dormant grape immersions were tested for control of mealybug planococcus ficus (signoret), on dormant grape cuttings used for nursery stock. a range of hot-water temperatures (47-58 degrees c) were evaluated at immersion periods of 2, 5, 10, or 20 min, by using a total of 353,720 mealybugs across all treatments. a 5-min immersion at 51 degrees c is effective in killing > 99% of p. ficus. at or above this immersion period and temperature, there was no difference in mealybug stage mortality. we evalua ...200516156560
effects of temperature and molecular oxygen on the use of atmospheric pressure plasma as a novel method for insect control.helium atmospheric pressure plasma discharge (appd) was previously shown to have insecticidal activity with a possible site of action on the insect nervous, neuromuscular system, or both. in the current study, methods to increase the insecticidal activity of plasma by using increased appd temperature and the introduction of molecular oxygen were investigated for the first time. an increase in the helium plasma temperature from 37 to 50 degrees c increased the insecticidal activity of plasma for ...200818459392
mode of action of a novel nonchemical method of insect control: atmospheric pressure plasma discharge.atmospheric pressure plasma discharge (appd) has been applied to a number of industrial applications, including the bacterial sterilization of medical equipment of bacteria. appd may also have applications in insect control. a positive correlation was found between exposure time to appd and mortality of western flower thrips, frankliniella occidentalis (pergande); tobacco thrips, frankliniella fusca (hinds); asian tiger mosquito, aedes albopictus (skuse); twospotted spider mite, tetranychus urti ...200616573321
biodegradation of benzene and its derivatives by a psychrotolerant and moderately haloalkaliphilic planococcus sp. strain zd22.the potential for biodegradation of aromatic hydrocarbons simultaneously at low temperatures and under saline and alkaline conditions is not well understood, but such biodegradation would be useful for remediation of polluted sites. a psychrotolerant, moderately haloalkaliphilic pure culture using benzene as a sole source of carbon and energy was isolated by selective enrichment from alkaline and saline soils in the vicinity of the daqing oil field in china. an analysis of the 16s rdna gene sequ ...200616815683
[toxicity screening and identification of bacteria isolated from snails nassarius semiplicatus and their habitat].tetrodotoxin and its analogues (ttxs) were responsible for the poisoning incidents associated with snail nassarius spp. we studied bacteria isolated from toxic snails as well as their habitat to probe into the relationship between bacteria and toxicity of nassariid gastropod.200818837369
transmission of grapevine leafroll-associated virus 3 by the vine mealybug (planococcus ficus).grapevine leafroll disease is caused by grapevine leafroll-associated viruses (glravs). within this virus complex, glrav-3 is the predominant species in the world. several glravs have been shown to be transmitted from vine to vine by mealybugs although a detailed characterization of transmission biology is lacking. the introduction of the vine mealybug (planococcus ficus) in california and other regions of the world may result in increasing disease incidence of established glravs. we studied the ...200818943455
inhibition of map kinases and down regulation of tnf-alpha, il-beta and cox-2 genes by the crude extracts from marine bacteria.crude ethyl acetate extracts from marine bacterial isolates staphylococcus arlettae kp2 (genbank accession no. eu594442) and planococcus maritimus kp8 (genbank accession no. eu594443) isolated from andaman seas were studied for their anti-inflammatory effect by lymphocyte proliferation assay (lpa) employing peripheral blood mononuclear cells (pbmcs). the crude extracts from both the bacteria down regulated the synthesis of inflammatory mediators such as tumor necrosis factor-alpha (tnf-alpha), i ...200918996678
biological control of grey mould in strawberry fruits by halophilic bacteria.grey mould caused by botrytis cinerea is an economically important disease of strawberries in tunisia and worldwide. the aim of this study was to select effective halophilic bacteria from hypersaline ecosystems and evaluate the abilities of antifungal bacteria to secrete extracellular hydrolytic enzymes, anti-botrytis metabolites and volatiles.200919191973
[bacterial communities of brown and red algae from peter the great bay, the sea of japan].the structure of microbial communities of brown algae, red algae, and of the red alga gracilaria verrucosa, healthy and affected with rotten thallus, were comparatively investigated; 61 strains of heterotrophic bacteria were isolated and characterized. most of them were identified to the genus level, some vibrio spp., to the species level according to their phenotypic properties and the fatty acid composition of cellular lipids. the composition of the microflora of two species of brown algae was ...200616871810
pheromone-based mating disruption of planococcus ficus (hemiptera: pseudococcidae) in california vineyards.experiments were conducted to test a mating disruption program for the mealybug planococcus ficus (signoret) (hemiptera: pseudococcidae) in california vineyards. the sprayable, microencapsulated formulation of the racemic sex pheromone lavandulyl senecioate was applied with an air-blast sprayer, using three and four applications in 2003 and 2004, respectively. mating disruption was combined with an application of buprofezin (2004) in june. compared with a no-pheromone control, there were signifi ...200616937683
effect of insecticides on mealybug destroyer (coleoptera: coccinellidae) and parasitoid leptomastix dactylopii (hymenoptera: encyrtidae), natural enemies of citrus mealybug (homoptera: pseudococcidae).in this study, we measured, under laboratory conditions, the direct and indirect effects of insecticides on mealybug destroyer, cryptolaemus montrouzieri mulsant (coleoptera: coccinellidae), and parasitoid leptomastix dactylopii howard (hymenoptera: encyrtidae), natural enemies of citrus mealybug, planococcus citri (risso) (homoptera: pseudococcidae). the adult stages of both natural enemies were exposed to sprays of the insecticides buprofezin, pyriproxyfen, flonicamid, acetamiprid, dinotefuran ...200617066788
[bacterial communities in the phase ii of agaricus bisporus compost by denaturing gradient gel electrophoresis].we studied the bacterial community during agaricus bisporus composting phase ii, to develop a quick and accurate method by modern molecular ecology technique for dynamic inspection on bacterial community during a. bisporus composting.200919445179
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