| phylogenetic analysis of the genera planococcus, marinococcus and sporosarcina and their relationships to members of the genus bacillus. | a phylogenetic analysis based on 16s rrna was performed on the genera planococcus, marinococcus, sporosarcina and endospore-forming rods. in agreement with earlier 16s rrna cataloguing data, planococcus citreus and sporosarcina ureae clustered with bacillus pasteurii and other bacilli containing lysine in their cell walls. sporosarcina halophila was shown to be genetically distinct from s. ureae and formed a loose association with the main bacillus subtilis grouping. marinococcus halophilus (for ... | 1992 | 1505740 |
| survey of metal tolerance in moderately halophilic eubacteria. | the tolerance patterns, expressed as mics, for 250 moderately halophilic eubacteria to 10 heavy metals were surveyed by using an agar dilution method. the moderate halophiles tested included 12 culture collection strains and fresh isolates representative of deleya halophila (37 strains), acinetobacter sp. (24 strains), flavobacterium sp. (28 strains), and 149 moderately halophilic gram-positive cocci included in the genera marinococcus, sporosarcina, micrococcus, and staphylococcus. on the basis ... | 1989 | 2802612 |
| phylogenetic characterization of a novel salt-tolerant bacillus species: description of bacillus dipsosauri sp. nov. | the taxonomic position of a novel halophilic endospore-forming bacterium previously isolated from a desert iguana was investigated by 16s rrna gene sequencing. comparative sequence analyses showed the unidentified bacterium to be phylogenetically loosely associated with some other spore-forming (bacillus pantothenticus, sporosarcina halophila) and non-spore-forming (marinococcus albus) halotolerant bacteria. based on the phenotypic and phylogenetic distinctiveness of the unidentified bacterium, ... | 1996 | 8675481 |
| characterization of genes for the biosynthesis of the compatible solute ectoine from marinococcus halophilus and osmoregulated expression in escherichia coli. | the genes of the biosynthetic pathway of ectoine (1,4,5,6-tetrahydro-2-methyl-4-pyrimidinecarboxylic acid) from the gram-positive moderate halophile marinococcus halophilus were cloned by functional expression in escherichia coli. these genes were not only expressed, but also osmoregulated in e. coli, as demonstrated by increasing cytoplasmic ectoine concentration in response to medium salinity. sequencing of a 4.4 kb fragment revealed four major orfs, which were designated ecta, ectb, ectc and ... | 1997 | 9141677 |
| identification of plasmids in the genus marinococcus and complete nucleotide sequence of plasmid ppl1 from marinococcus halophilus. | several plasmids were detected in the gram-positive halophilic eubacterium marinococcus halophilus and in the related strain m52. the complete nucleotide sequence (3874 bp) of one of these plasmids, ppl1, was determined. four major open reading frames were identified. whereas orf3 and orf4 showed no sequence similarities to known proteins, rep displayed a high sequence similarity to replication proteins of rolling circle plasmids. upstream of this orf, a sequence resembling the double-strand ori ... | 1997 | 9339468 |
| construction and characterization of an nacl-sensitive mutant of halomonas elongata impaired in ectoine biosynthesis. | using transposon mutagenesis we generated a salt-sensitive mutant of the halophilic eubacterium halomonas elongata impaired in the biosynthesis of the compatible solute ectoine. hplc determinations of the cytoplasmic solute content showed the accumulation of a biosynthetic precursor of ectoine, l-2,4-diaminobutyric acid. ectoine and hydroxyectoine were not detectable. this mutant failed to grow in minimal medium with nacl concentrations exceeding 4%. however, when supplemented with organic osmol ... | 1998 | 9570121 |
| gracilibacillus gen. nov., with description of gracilibacillus halotolerans gen. nov., sp. nov.; transfer of bacillus dipsosauri to gracilibacillus dipsosauri comb. nov., and bacillus salexigens to the genus salibacillus gen. nov., as salibacillus salexigens comb. nov. | a gram-positive, extremely halotolerant bacterium was isolated from the great salt lake, utah, usa. the strain, designated nnt (= dsm 11805t), was strictly aerobic, rod-shaped, motile by peritrichous flagella and spore-forming. strain nnt grew at salinities of 0-20% (w/v) nacl. a distinctive feature of strain nnt was its optimal growth in salt-free medium. the polar lipid pattern of strain nnt consisted of phosphatidyl glycerol, diphosphatidyl glycerol and two phospholipids of unknown structure. ... | 1999 | 10319508 |
| biomineralization of carbonates by marinococcus albus and marinococcus halophilus isolated from the salar de atacama (chile). | we studied the precipitation of carbonates in 17 strains of moderately halophilic, gram-positive cocci belonging to two species: marinococcus halophilus and marinococcus albus, isolated from the salar de atacama (chile). they were cultivated in solid and liquid laboratory media for 42 days at salt concentrations (wt/vol) of 3%, 7.5%, 15%, and 20%. the bioliths precipitated were studied by x-ray diffraction and scanning electron microscopy. m. halophilus formed crystals at each of the salt concen ... | 1999 | 10387118 |
| extrinsic protein stabilization by the naturally occurring osmolytes beta-hydroxyectoine and betaine. | thermodynamic aspects of protein stabilization by two widespread naturally occurring osmolytes, beta-hydroxyectoine and betaine, were studied using differential scanning calorimetry (dsc) and bovine ribonuclease a (rnase a) as a model protein. the osmolyte beta-hydroxyectoine purified from marinococcus was found to be a very efficient stabilizer. at a concentration of 3m it increased the melting temperature of rnase a (tm) by more than 12k and gave rise to a stability increase of 10.6kj/mol at r ... | 1999 | 10484175 |
| filobacillus milensis gen. nov., sp. nov., a new halophilic spore-forming bacterium with orn-d-glu-type peptidoglycan. | a spore-forming, halophilic bacterium was isolated from surface sediment located on the beach of palaeochori bay near to a shallow water hydrothermal vent area, milos, greece. the bacterium, designated sh 714t, consisted of motile, strictly aerobic rods which contained an orn-d-glu type murein and a g+c content of 35 mol%. thin sections showed a cell wall typical for gram-positive bacteria; the peptidoglycan layer, however, was very thin. the gram-reaction of the organism was negative. comparati ... | 2001 | 11324591 |
| investigation into a stress-inducible promoter region from marinococcus halophilus using green fluorescent protein. | a 480-bp marinococcus halophilus dna-fragment upstream of the ectoine genes ectabc was linked to the reporter gene gfp(uv) in the stress probe plasmid pbrgfp(uv). on the basis of green fluorescence emitted after application of different types of stress to recombinant escherichia coli, the dna sequence was characterized as a stress-responding promoter region. in e. coli, osmotic stress resulted in an increasing fluorescence intensity, whereas the effects of heat or cold were insignificant, sugges ... | 2002 | 11878557 |
| diversity of moderately halophilic bacteria producing extracellular hydrolytic enzymes. | the aim of this study was to determine the diversity of moderately halophilic bacteria with hydrolase activities. | 2003 | 12534822 |
| marinococcus halophilus dsm 20408t encodes two transporters for compatible solutes belonging to the betaine-carnitine-choline transporter family: identification and characterization of ectoine transporter ectm and glycine betaine transporter betm. | in response to osmotic stress, the halophilic, gram-positive bacterium marinococcus halophilus accumulates compatible solutes either by de novo synthesis or by uptake from the medium. to characterize transport systems responsible for the uptake of compatible solutes, a plasmid-encoded gene bank of m. halophilus was transferred into the transport-deficient strain escherichia coli mkh13, and two genes were cloned by functional complementation required for ectoine and glycine betaine transport. the ... | 2004 | 14872322 |
| rapid screening and dereplication of bacterial isolates from marine sponges of the sula ridge by intact-cell-maldi-tof mass spectrometry (icm-ms). | rapid grouping of bacterial isolates is critical in comprehensive microbial studies of environmental samples or screening programmes e.g. in unknown marine environments where large numbers of strains have to be isolated on different growth media. sets of bacteria have been cultured from the marine sponges isops phlegraei, haliclona sp. 1, phakellia ventilabrum and plakortis sp. growing at a depth of about 300 m on the sula ridge close to the norwegian coast. we employed intact-cell maldi-tof (ic ... | 2004 | 15614563 |
| marinococcus halotolerans sp. nov., isolated from qinghai, north-west china. | an aerobic bacterium was isolated from saline soil located in qinghai, north-west china. the bacterium, designated yim 70157(t), was investigated using a polyphasic taxonomic approach. the gram reaction of the organism was positive. comparative 16s rrna gene sequence analysis demonstrated the isolate to be a member of the genus marinococcus, the closest phylogenetic neighbour of the unknown bacterium being marinococcus halophilus dsm 20408(t) with a similarity of 99.4%. the peptidoglycan type of ... | 2005 | 16166669 |
| identification of sulfoquinovosyl diacylglycerol as a major polar lipid in marinococcus halophilus and salinicoccus hispanicus and substitution with phosphatidylglycerol. | the sulfonolipid sulfoquinovosyl diacylglycerol normally associated with photosynthetic membranes was identified as a major lipid in marinococcus halophilus, salinicoccus hispanicus ("marinococcus hispanicus"), and marinococcus sp. h8 (planococcus sp. h8). phosphatidylglycerol and 0%-10% cardiolipin accounted for the remaining polar lipids in these moderately halophilic, gram-positive bacteria. negative-ion fast atom bombardment mass spectrometry was used to quantify these three polar lipids fro ... | 2006 | 16604117 |
| sinococcus qinghaiensis gen. nov., sp. nov., a novel member of the order bacillales from a saline soil in china. | a gram-positive, non-spore-forming isolate, designated yim 70212(t), was isolated from a hypersaline soil sample collected from qinghai, north-west china. cells of the isolate were orange-pigmented, motile cocci with multiple flagella. a polyphasic taxonomic investigation was carried out on the isolate. the organism grew at 10-45 degrees c and ph 7.5-11.0, with optimum growth at 28 degrees c and ph 8.0-9.5. strain yim 70212(t) grew optimally in the presence of 10 % nacl, kcl or mgcl(2).6h(2)o an ... | 2006 | 16738089 |
| high-yield cultivation of marinococcus m52 for production and recovery of hydroxyectoine. | ectoine and hydroxyectoine, produced by halomonas ssp. and marinococcus ssp., have been extensively characterized and proposed for biotechnological applications. large-scale production of ectoine was achieved by implementing the so-called "milking process" by bitop (witten, germany) and commercializing compatible solutes as stabilizers for biological systems. however, there is a continuing interest in optimization of the production process for hydroxyectoine alone, as the latter was shown to hav ... | 2006 | 16815686 |
| cloning and characterization of the genes for biosynthesis of the compatible solute ectoine in the moderately halophilic bacterium halobacillus dabanensis d-8(t). | a 11.2-kb fragment containing the ectabc genes of the biosynthetic pathway of ectoine from the gram-positive, moderately halophilic bacterium halobacillus dabanensis d-8(t) was obtained by inverse polymerase chain reaction. subsequently, the entire ectabc cluster was cloned and analyzed. it revealed that the intergenic regions of the ectabc genes from h. dabanensis d-8(t) are more tightly spaced than those of chromohalobacter salexigens, halomonas elongata, marinococcus halophilus, and salibacil ... | 2006 | 16874550 |
| [taxonomic composition of bacteria associated with cultivated mollusks crassostrea lugubris and perna viridis and with the water of the gulf of nha trang lagoon, vietnam]. | one hundred and four strains of heterotrophic bacteria have been isolated and characterized from two species of bivalve mollusks cultivated in the gulf of nha trang (vietnam) and from the water of a mariculture farm. the isolates have been identified on the basis of morphological, physiological, biochemical, and chemotaxonomic properties, as well as by the content of g+c bases in dna. in the microflora of mollusks, vibrio alginolyticus was predominant; the pathogenic species v. harveyi and v. sp ... | 2007 | 17583223 |
| salsuginibacillus kocurii gen. nov., sp. nov., a moderately halophilic bacterium from soda-lake sediment. | a gram-positive, endospore-forming, alkali-tolerant, moderately halophilic bacterium, designated strain ch9d(t), was isolated from the sediment of lake chagannor in the inner mongolia autonomous region, china. the cells were rod-shaped and motile. isolate ch9d(t) grew at ph 5.8-10.0 (optimally at ph 8.5), at salinities of 3-20 % (w/v) marine salts (optimally at 10.0 %, w/v) and between 20 and 50 degrees c (optimally at 37 degrees c). the cell wall contained meso-diaminopimelic acid and the major ... | 2007 | 17911315 |
| reclassification of marinococcus albus hao et al. 1985 as salimicrobium album gen. nov., comb. nov. and bacillus halophilus ventosa et al. 1990 as salimicrobium halophilum comb. nov., and description of salimicrobium luteum sp. nov. | a gram-positive, non-motile, coccoid-shaped, non-spore-forming halophilic bacterial strain, by-5(t), was isolated from a marine solar saltern in korea and its taxonomic position was investigated by using a polyphasic approach. the novel strain grew optimally at 37 degrees c and in the presence of 10 % (w/v) nacl. strain by-5(t) had meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan, mk-7 as the predominant menaquinone and anteiso-c(15 : 0), iso-c(15 : 0), ante ... | 2007 | 17911318 |
| heterologous ectoine production in escherichia coli: by-passing the metabolic bottle-neck. | transcription of the ectoine biosynthesis genes ecta, ectb and ectc from marinococcus halophilus in recombinant escherichia coli dh5alpha is probably initiated from three individual sigma70/sigmaa-dependent promoter sequences, upstream of each gene. consequently, mrna-fragments containing the single genes and combinations of the genes ecta and ectb or ectb and ectc, respectively, could be detected by northern blot analysis. under the control of its own regulatory promoter region (ectup) a seemin ... | 2008 | 18759971 |
| a novel peptidoglycan recognition protein containing a goose-type lysozyme domain from the pacific oyster, crassostrea gigas. | peptidoglycan recognition protein (pgrp) is considered an essential molecule for effective immunity in invertebrates by its detection and clarification of invading bacteria. bivalve mollusks also possess pgrp systems for self-defense, however, their functions in bivalves remain to be understood. in the present study, cdna of a novel pgrp was identified from the pacific oyster, crassostrea gigas, using est-based race pcr. this novel pgrp is homologous to short pgrps and the presence of a signal p ... | 2009 | 19246096 |
| marinococcus luteus sp. nov., a halotolerant bacterium isolated from a salt lake, and emended description of the genus marinococcus. | a gram-positive, aerobic, motile, coccoid, orange-pigmented bacterium, designated strain yim 91094(t), was isolated from a salt lake sample collected from barkol lake in xinjiang province, north-west china. the strain was able to grow at ph 6.0-8.0 (optimal growth at ph 7.0), at 10-37 degrees c (optimal growth at 28 degrees c) and in the presence of 0-25 % (w/v) nacl [optimal growth in the presence of 10-15 % (w/v) nacl]. phylogenetic analyses based on 16s rrna gene sequences showed that strain ... | 2009 | 19628611 |
| studies on the biodiversity of halophilic microorganisms isolated from el-djerid salt lake (tunisia) under aerobic conditions. | bacterial and archaeal aerobic communities were recovered from sediments from the shallow el-djerid salt lake in tunisia, and their salinity gradient distribution was established. six samples for physicochemical and microbiological analyses were obtained from 6 saline sites in the lake for physico-chemical and microbiological analyses. all samples studied were considered hypersaline with nacl concentration ranging from 150 to 260 g/l. a specific halophilic microbial community was recovered from ... | 2009 | 20066169 |
| production and characterization of ectoine by marinococcus sp. ect1 isolated from a high-salinity environment. | a halophilic bacterium isolated from a salt environment in southern taiwan was identified as a marinococcus sp. ect1. this bacterium could synthesize and accumulate intracellular ectoine as a compatible solute capable of resisting osmotic stress in a hyper-osmotic environment. this study also developed a semi-synthesized medium (yams medium), capable of facilitating the growth of this marinococcus sp. ect1 with 600 mg/l crude ectoine production. moreover, marinococcus sp. ect1 was grown on yams ... | 2010 | 21159550 |