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enterococcus columbae, a species from pigeon intestines.gram-positive cocci which dominate in the intestinal flora of domestic pigeons were found to constitute a new species of the genus enterococcus. the strains were most closely related to enterococcus cecorum, originally described as streptococcus cecorum, a carboxyphilic species from chicken intestines, and to enterococcus avium. the pigeon strains resemble e. cecorum and also many e. avium strains in their lack of group d antigen and in being more sensitive to nacl than other enterococci. the ty ...19902272492
sequencing the gene encoding manganese-dependent superoxide dismutase for rapid species identification of enterococci.simple pcr and sequencing assays that utilize a single pair of degenerate primers were used to characterize a 438-bp-long dna fragment internal (soda(int)) to the soda gene encoding the manganese-dependent superoxide dismutase in 19 enterococcal type strains (enterococcus avium, enterococcus casseliflavus, enterococcus cecorum, enterococcus columbae, enterococcus dispar, enterococcus durans, enterococcus faecalis, enterococcus faecium, enterococcus flavescens, enterococcus gallinarum, enterococc ...200010618129
evidence for horizontal gene transfer in evolution of elongation factor tu in enterococci.the elongation factor tu, encoded by tuf genes, is a gtp binding protein that plays a central role in protein synthesis. one to three tuf genes per genome are present, depending on the bacterial species. most low-g+c-content gram-positive bacteria carry only one tuf gene. we have designed degenerate pcr primers derived from consensus sequences of the tuf gene to amplify partial tuf sequences from 17 enterococcal species and other phylogenetically related species. the amplified dna fragments were ...200011092850
composition of enterococcal and streptococcal flora from pigeon intestines.to identify the streptococcal and enterococcal components of the microflora from pigeon intestines to species level by tdna-intergenic polymerase chain reaction.200211849364
comparison of susceptibility to antimicrobials of the enterococcal species isolated from pigeons (columba livia).antimicrobial resistance profiles were assessed in enterococcus faecium, enterococcus faecalis, and enterococcus columbae strains isolated from racing pigeons (columba livia). e. columbae is the most prevalent enterococcal species in the intestines of pigeons. acquired resistance against the antimicrobials bambermycin, vancomycin, monensin, narasin, virginiamycin (cross-resistant with quinupristin/dalfopristin), avilamycin (cross-resistant with evernimicin), and ampicillin was not seen. only for ...200212363011
genes encoding bacteriocins and their expression and potential virulence factors of enterococci isolated from wood pigeons (columba palumbus).samples of the intestinal content and carcasses of wood pigeons (columba palumbus) were evaluated for enterococci with antimicrobial activity. enterococcus faecium comprised the largest enterococcal species with antagonistic activity, followed by enterococcusfaecalis and enterococcus columbae. pcr amplification of genes coding bacteriocins and determination of their nucleotide sequence, and the use of specific antipeptide bacteriocin antibodies and a noncompetitive indirect enzyme-linked immunos ...200616541681
macedovicin, the second food-grade lantibiotic produced by streptococcus macedonicus aca-dc 198.streptococcus macedonicus aca-dc 198 was found to produce a second lantibiotic named macedovicin in addition to macedocin. macedovicin was purified to homogeneity and mass spectrometric analysis identified a peptide of approximately 3.4 kda. partial n-terminal sequence analysis and tandem mass spectrometry revealed that macedovicin was identical to bovicin hj50 and thermophilin 1277 produced by streptococcus bovis and streptococcus thermophilus, respectively. macedovicin inhibits a broad spectru ...201323122510
presence and mechanism of macrolide-lincosamide resistance in enterococcus columbae strains belonging to the intestinal flora of pigeons.faecal samples from 50 pigeons all originating from different lofts were screened for the presence of macrolide and lincosamide (ml)-resistant isolates of streptococcus gallolyticus and enterococcus columbae by plating the samples onto selective media. sixty-eight ml-resistant e. columbae strains were recovered from the faecal samples of 29 animals. two of these samples also harboured ml-resistant s. gallolyticus strains. the erm(b) gene was detected in 58 e. columbae and in five s. gallolyticus ...200415223559
identification of enterococci at the species level by sequencing of the genes for d-alanine:d-alanine ligases.a pcr assay based on the use of degenerate oligodeoxyribonucleotides allowed characterization of a fragment internal to the ddl genes encoding d-alanine:d-alanine ligases in enterococcus columbae, e. durans, e. malodoratus, e. mundtii, e. raffinosus, e. seriolicida, e. solitarius, and e. sulfureus. phylogenetic analysis of the sequence of the amplification products and of those already obtained from e. aviuni, e. casseliflavus, e. cecorum, e. dispar, e. faecalis, e. faecium, e. flavescens, e. ga ...200010930075
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