Publications

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relationship between serum antibiotic concentration and inhibiting potency of the serum. application to ampicillin and amoxicillin. 1975654
inhibition of bacterial wall lysins by lipoteichoic acids and related compounds. 19751027
microbiological study of gentamiycin.gentamycin prepared at the all-union research institute of antibiotics did not differ by its antibacterial spectrum and the activity level from gentamycin samples from other countries. by its activity against clinical strains of ps. aeruginosa gentamycin was somewhat inferior than polymyxin but much more superior than carbenicillin. an agar-diffusion method using bac. pumilus ntcc 8241 as the test microbe was developed for determination of gentamycin activity. the gentamycin sulfate complex and ...19752095
the interrelationship between the inhibitory activity of milk with different types of beta-lactoglobulins and the resistance of cattle to mastitis.in the course of the investigation of piebald (black-white) cattle it is found that 17,62% animals produce the aa type beta-lactoglobulin, 49,52%--the ab type and 32,86%--the bb type. the higher inhibitory activity of milk with the bb type beta-lactoglobulin was found which retained in dilution 1 : 32. the total flora of teat, cyst and parenchyma milk of animals with the bb type beta-lactoglobulin as well as enterococcus bacteria were much lower than in milk of cows with the aa type homogenous f ...19755340
gentamicin-susceptibility of various pathogens isolated from clinical materials.we studied on the antibacterial activity of gentamicin against various pathogens isolated from clinical materials mainly isolated during 1974 and 1975, comparing with other antibiotics. beta hemolytic streptococci, pneumococci and enterococci are less susceptible to gentamicin than staphylococci. staph, aureus and staph. epidermidis resistant to various antibiotics are very susceptible to gentamicin, and no resistant strain to this drug was found. haemophilus influenzae, h. parainfluenzae and h. ...19765619
bactericidal mechanisms in rabbit alveolar macrophages: evidence against peroxidase and hydrogen peroxide bactericidal mechanisms.the role of peroxidase-mediated bacterial killing by rabbit alveolar macrophages was examined. during 3 h of incubation in vitro, alveolar macrophages ingested and killed greater than 88% of the streptococcus faecalis, proteus mirabilis, or streptococcus pneumoniae present in the incubation mixture. preincubation of alveolar macrophages with inhibitors of catalase, 3-amino-1,2,4-triazole or sodium nitrite, did not alter their bactericidal potential. iodination of ingested zymosan particles, a pe ...19767533
approach to a practical method for screening and identifying microorganism genera from urine (author's transl).in this study the author reported upon a practical new system for screening and identifying the microbial agents causing urinary tract infections. this system is composed of a combination of 3 screening procedures (ph-value + nitrite-test + catalase-test) and 8 selective culture media for the purpose of genus identification within 24 hours (uripret-g). a total of 130 cultures was investigated. the employed microorganisms were mainly recovered from urine samples. they included the following speci ...197611179
the role of microflora in development of graft-versus-host disease. 197611583
resistance mechanism of chloramphenicol in streptococcus haemolyticus, streptococcus pneumoniae and streptococcus faecalis.the chloramphenicol resistance of streptococcus haemolyticus, streptococcus pneumoniae and streptococcus faecalis isolated from clinical materials was proved to be due to an inactivating enzyme produced by these bacteria. the inactivated products of chloramphenicol were identified as 1-acetoxy, 3-acetoxy and 1,3-diacetoxy derivatives by thin-layer chromatography and infrared spectroscopy. the responsible enzyme was thus confirmed to be chloramphenicol acetyltransferase. the enzyme was inducible. ...197716197
the exchange reaction of peptides r-d-alanyl-d-alanine with d-14calanine to r-d-alanyl-d-14calanine and d-alanine, catalysed by the membranes of streptococcus faecalis atcc 9790.under alkaline conditions, the membrane-bound dd-carboxypeptidase of streptococcus faecalis atcc 9790 catalyses exchange reactions in which the x-l-r3-d-ala moiety of peptides of the type x-l-r3-d-ala-d-ala is transferred to simple amino compounds such as d-alanine, glycine and glycyl-glycine. the enzyme system is unable, however, to catalyse complex reactions that would simulate the natural transpeptidation reaction.197716752
kinetics and equilibrium of the inactivation of ornithine transcarbamylases by pyridoxal 5'-phosphate. 197716927
bacterial endocarditis: clinical and bacteriological aspects and prognostic factors.the microbiological, clinical and therapeutic aspects of all (71) cases of bacterial endocarditis admitted to the hôpital cantonal, geneva, between august 1970 and october 1974 were reviewed: there was a definite trend towards higher prevalence of acute cases compared to subacute cases. the pathogenic role of s. epidermidis, mostly in cases of prosthetic valve endocarditis, could be demonstrated in several cases. when defined initially by microbiological criteria only, acute endocarditis were ch ...197717156
microbiological measurement of folic coenzymes. ii. effect of the ph of ascorbic acid. 197723813
effects of temperature on the autolytic enzyme system of streptococcus faecalis.the cellular autolytic reaction system in streptococcus faecalis atcc 9790 was analyzed for relative increases in reaction rates with increasing temperature by determination of arrhenius activation energies (e). the systems examined were: (i) an isolated wall-enzyme complex in 0.01 m sodium phosphate, ph 6.9; (ii) exponential-phase cells suspended in 0.01 or o.3 m sodium phosphate ph 6.8, or in 0.04 m ammonium acetate, ph 6.8, (iii) growing cultures deprived of glucose or lysine; and (iv) cultur ...197830752
[changes in the number of escherichia coli, streptococcus faecalis and streptococcus faecium stored in physiological solution at a temperature of 20 degrees c]. 197731606
[enterocins and their properties]. 197934298
attachment of bacteria to exfoliated cells from the urogenital tract.to establish urogenital infections, organisms must adhere to the mucosal lining. a differential adherence capacity among various bacterial species was observed when exfoliated urethral and urothelial cells were tested in an in vitro system. no difference in the adherence capacity of a particular species was observed when tested with exfoliated cells obtained from voided urine from different healthy individuals of the same sex. escherichia coli harvested directly from urine specimens of patients ...197934577
effect of storage at 1 degree and 4 degrees c on viability and injury of staphylococcus aureus, escherichia coli and streptococcus faecalis. 197935509
magnitude of pollution indicator organisms in rural potable water.a total of 460 water samples were randomly drawn from the potable water supply sources of rural communities in three counties of south carolina. about 10% of the population, not incorporated in municipalities, was sampled. the samples were tested for total coliforms, escherichia coli, and fecal streptococci. significant levels of these pollution indicator organisms were detected in almost all the water supplies. total coliforms were the most common, and only 7.5% of the water supplies were uncon ...197936849
organization and transfer of heterologous chloramphenicol and tetracycline resistance genes in pneumococcus.the cat and tet genes of chloramphenicol- and tetracycline-resistant clinical isolates of streptococcus pneumoniae from paris and japan were shown to be contained in adjacent heterologous insertions into the chromosome. the two insertions transformed laboratory strains at frequencies that were low, unequal, and, for tet, very sensitive to the length of the donor deoxyribonucleic acid strand. in contrast, the transforming activity of cat was relatively stable. there was an unusual asymmetric cotr ...197937238
reduction of fecal indicator bacteria in sewage effluent when pumping for crop irrigation.when sewage effluent was pumped from a lagoon for spray irrigation the pumping process reduced the fecal coliforms (fc) by 66% and the fecal streptococci (fs) by 91%. bactericidal effects associated with the pumping of effluent were investigated in the laboratory by seeding sterilized effluent with fecal bacteria and repeatedly passing the liquid through a centrifugal impeller type pump at 50 psi pressure. the pumping effect was cumulative, 49 and 90% of fc being killed after two and four passag ...197938272
does penicillin kill bacteria?.the thesis is presented that the bactericidal action of penicillin and of other inhibitors of cell wall peptidoglycan synthesis, such as vancomycin and cycloserine, is secondary or tertiary to their ability inhibit specific reactions in the assembly of an osmotically protective cell wall. examples are given of the inhibition of these reactions, which results in inhibition of cell growth (bacteriostatic action) in the absence of either cellular lysis or rapid loss of viability. thus, in some inst ...197944383
an electron microscopic study of the location of teichoic acid and its contribution to staining reactions in walls of streptococcus faecalis 8191.the location of the glucosylated teichoic acid in whole cells and isolated walls of streptococcus faecalis 8191 has been investigated using ruthenium red, gold-labelled concanavalin a and concanavalin a-peroxidase-diaminobenzidine. dense laminae were revealed in sections of osmium-fixed walls stained with ruthenium red which corresponded to similar regions stained by uranyl and lead. such regions were not seen after teichoic acid had been extracted, suggesting that the uptake of stain was by tei ...197550406
photobiological behaviour of bacteria and phages supplemented with aza-analogoues of nucleic acid bases.the photochemical stability of anomalous nucleic acid bases of the azatype, 5-azacytosine (i), 5-azacytidine (ii), 6-azacytosine (iii), 6-azacytidine (iv), 6-azathymine (v), 6-azauracil (vi), and 8-aza-adenine (vii) to u. v. light of the wavelength 254 nm differs from the u. v. stability of the normal constituents. changes of the u.v. inactivation of escherichia coli k12 c600, e. coli b, bacillus cereus, as well as e. coli phages gamma cb2 and gamma b2b5 supplemented with azaderivatives prior to ...197552947
effects of phenethyl alcohol on bacillus and streptococcus.the activity of phenethyl alcohol (pea) on bacillus cereus, b. megaterium, and streptococcus faecalis was studied by electron microscopy of thin sections and by the assay of intracellular k+ leakage. s. faecalis was unaffected by pea at concentrations up to 0.5%, b. cereus was severely damaged by 0.5% pea, and b. megaterium behaved intermediately. important membrane ultrastructural alterations were observed in b. cereus cells treated with 0.5% pea, namely the change in the geometry of the membra ...197660333
aldolases of the lactic acid bacteria. demonstration of immunological relationships among eight genera of gram positive bacteria using an anti-pediococcal aldolase serum.reciprocal qualitative and quantitative immunological experiments employing an anti-pediococcus cerevisiae aldolase serum confirmed many of the interspecific relationships demonstrated previously among lactic acid bacteria with antisera prepared against the streptococcus faecalis fructose diphosphate aldolase. the extent of immunological relatedness observed between the lactobacillus and pediococcus aldolases was markedly gses indicating that the pediococci share closer phylogenetic ties with th ...197665160
identification of the presence and type of biliary microflora by immediate gram stains.immediate gram stains were performed on gallbladder bile aspirated at the start of an operation for biliary disease in 191 consecutive patients undergoing elective biliary surgery. the results of the gram stains were telephoned to the operating theater within 20 minutes of collection. the over-all accuracy rate of the telephone gram stain reports compared with the subsequent bile cultures was 77 percent. the incidence of false-positive results was 12 percent, and false-negative results were reco ...197766763
chemical modification of peptide antibiotics: part iv--biologically active synthetic peptides & derivatives. 197667080
prophylactic systemic antibiotics in colorectal surgery.the prophylactic value of gentamicin combined with either lincomycin or metronidazole in 52 patients undergoing colorectal surgery was investigated. the results confirmed the value of this practice. in a control group, the sepsis-rate was 48% with 1 death attributable to sepsis, compared with a sepsis-rate of 4% in the treated group. the combination of gentamicin and lincomycin was effective against sepsis but pseudomembranous colitis developed in 2 of the 14 patients treated with this combinati ...197769126
[modification of the method of bacteriological diagnosis of intestinal dysbacteriosis]. 197771381
chemical modification peptide antibiotics: part vii--synthetic peptides with sequences from gramicidin b & c. 197772723
phagocytosis in patients and carriers of chronic granulomatous disease.the phagocytic capacity of leucocytes from four patients and two carriers of chronic granulomatous disease (c.g.d.) was compared to that of normal leucocytes. leucocytes from c.g.d. patients phagocytised more staphylococcus aureus than did normal leucocytes after 5, 10, and 20 minutes of incubation. phagocytosis by leucocytes from two carriers of c.g.d. was intermediate between that in the patients and in controls. in contrast, phagocytosis of streptococcus faecalis, an organism readily killed b ...197548723
[characteristics of enterococci isolated from the pharynx, urine and the genital organs of pregnant women]. 197875300
transferable drug resistance to group a and group b streptococci. 197876183
immunohistochemical localization of a choriogonadotropin-like protein in bacteria isolated from cancer patients.by the use of specific antibody to human chorionic gonadotropin (cg) as well as to its beta-subunit, and the application of the indirect fluorescein-labeled and peroxidase-labeled antibody techniques, we have demonstrated the presence of a membrane (wall)-associated cg-similar immunoreactive protein in 15 strains of bacteria isolated from tissues of patients bearing malignant neoplasms. these microorganisms were classified as s. epidermidis, (12) e. coli (2), and a single strain of p. maltophili ...197876504
accumulation of arsenate, phosphate, and aspartate by sreptococcus faecalis.uptake of arsenate and phosphate by streptococcus faecalis 9790 is strictly dependent on concurrent energy metabolism and essentially unidirectional. targinine supports uptake only in presence of glycerol or related substances; glycerol is not directly involved in transport but depletes the cellular orthophosphate pool and thus relieves feedback inhibition of transport. uptake of phosphate and arsenate is stimulated by k+ and by other permeant cations. the results suggest that electroneutrality ...197547322
catheter-associated urinary-tract infections. 197882043
association between streptococcus faecalis urinary infections and graft rejection in kidney transplantation.in the first month after transplantation 50% of 193 consecutive renal transplant recipients had bacteriuria. the most common organisms isolated were streptococcus faecalis (34), escherichia coli (28), pseudomonas spp. (11), and staphylococcus (9). there was a significant correlation between infection with str. faecalis and graft failure at one, three, and twelve months. this observation suggests that urinary infection with str. faecalis may be associated with graft failure which is probably the ...197882139
teichoic acids of group d streptococci with special reference to strains from pig meningitis (streptococcus suis).immunoelectrophoresis revealed in phenol extracts from s. faecalis and s. faecium a mixture of free and lipid-bound teichoic acids, both reactive with group d antisera. in phenol extracts from s. suis only lipid-bound teichoic acid, also reactive with group d antiserum, was seen. this difference probably accounts for the low yield of group d antigen from s. suis as compared with s. faecalis and s. faecium when heating at ph 2 is used for extraction. when phenol is used good yields are obtained f ...197745589
[quaternary structure and mechanism of action of putrescine carbamoyltransferase (proceedings)]. 197884627
streptococcus faecalis urinary-tract infection and renal-allograft rejection. 197986016
interaction of a bivalent ligand with igm anti-lactose antibody. 197987219
a resonance raman and electronic absorption probe of membrane energization. quinaldine red in cells of streptococcus faecalis.resonance raman and electronic absorption spectra were used to show that the state of an amphiphilic cation, relative to dilute aqueous solution, changes when it is accumulated by cells of streptococcus faecalis when they are energized. the general characteristics of the cation employed, quinaldine red, closely paralleled those of other amphiphilic cations which have been used to measure membrane potential. a major aspect of the change is that in sodium-loaded cells, essentially all of the quina ...197939936
effect of leukocyte hydrolases on bacteria. xiv. bacteriolytic effects of human sera, synovial fluids, and purulent exudates on staphylococcus aureus and streptococcus faecalis: modulation by cohn's fraction ii and by polyelectrolytes.normal sera and plasma, derived from humans, calves, rats, rabbits, horses, human synovial fluids, inflammatory exudates, and leukocyte extracts, when sufficiently diluted are highly bacteriolytic for staph, aureus, strep. faecalis, b. sutilis and to a variety of gram-negative rods. on the other hand, concentrated serum or the other body fluids are usually not bacteriolytic for these bacterial species. while the lysis of staph, aureus and b. subtilis by diluted serum is not lysozyme dependent, l ...197992458
a study of the surface-active properties of the mg2+-activated atpase from cytoplasmic membranes of streptococcus faecalis.the surface activity and enzymic properties of the factor f1, the catalytic moiety of streptococcus faecalis h+-atpase, has been studied at the air-water and phospholipid-water interfaces. f1 does not interact with the monolayer phospholipids, hence its adsorption on a biological membrane must be due mainly to its recognition of proteins of the hydrophobic complex. the dimensions of the f1 molecule at the air-water interface have been estimated. in the presence of mg2+, base area is s = 1.8 . 10 ...197939596
stereospecificity of sodium borohydride reduction of tyrosine decarboxylase from streptococcus faecalis.sodium boro[3h]hydride reduction of tyrosine decarboxylase from streptococcus faecalis followed by complete hydrolysis of the enzyme produces epsilon-[3h]pyridoxyllysine. degradation of this material to [4'-3h]pyridoxamine and stereochemical analysis with apoaspartate aminotransferase shows that the re side at c-4' of the cofactor is exposed to solvent at ph 5.5 and 7.0. after binding of l-tyrosine at ph 5.5 or tyramine at ph 7.0 to the holoenzyme, sodium boro[3h]hydride reduction proceeds from ...197936380
effect of ionizing radiation on transmembrane potential of streptococcus. 197935814
[differential diagnosis of enterococci].the most stable differential signs of enterococci are: growth in the medium at ph 10.2, growth in broth containing 40% bile, citrate utilization, resistance to 0,05% potassium tellurite, 2, 3, 5-triphenyltetrazolium chloride (ttc) reduction, the staining of colonies (plaques) on a medium with manganese, iron and zinc salts, glycerine fermentation under anaerobic conditions, mannite fermentation, the presence of hemolysin, of the proteolytic enzyme, and mobility. combined differential-diagnostic ...197834304
the role of phosphatidylglycerol as a donor of phosphatidyl and of sn-glycerol-1-phosphate groups in biosynthetic reactions. 197896663
the antimicrobial activity of urine of paraplegic patients receiving methenamine mandelate.the antimicrobial activity of urine collected from adult male paraplegics ingesting methenamine mandelate (mm) was evaluated. the in vitro bacterial growth in urine from these patients was inhibited when the free formaldehyde (hcho) concentration was 10 to 22 microgram per ml. when the hcho concentration was in the region of 28 microgram per ml or greater, bactericidal effect became apparent. urine containing 1323 microgram of mm per ml with a ph of 5.9 when freshly voided had sufficient hcho to ...197830734
solubilization and isolation of the membrane-bound dd-carboxypeptidase of streptococcus faecalis atcc9790. properties of the purified enzyme.streptococcus faecalis atcc 9790 possesses six membrane-bound, penicillin-binding proteins. that numbered 6 (mr 43000) is the most abundant one and is the dd-carboxypeptidase studied previously. the enzyme has been solubilized and purified to the stage where one single protein band can be detected by gel electrophoresis. the purification procedure does not alter the properties that the enzyme exhibits when it is membrane-bound. the dd-carboxypeptidase itself may be a killing target for penicilli ...197897082
cellular lysis of streptococcus faecalis induced with triton x-100.lysis of exponential-phase cultures of streptococcus faecalis atcc 9790 was induced by exposure to both anionic (sodium dodecyl sulfate) and nonionic (triton x-100) surfactants. lysis in response to sodium dodecyl sulfate was effective only over a limited range of concentrations, whereas triton x-100-induced lysis occurred over a broad range of surfactant concentrations. the data presented indicate that the bacteriolytic response of growing cells to triton x-100: (i) was related to the ratio of ...197897265
use of furagin in treatment of urinary tract infections. 197825493
acid production from lycasin, maltitol, sorbitol and xylitol by oral streptococci and lactobacilli.the acid production from maltitol was compared with the acid production from hydrogenated starch hydrolysate (lycasin), sorbitol and xylitol by a number of oral strains and reference strains of streptococcus mutans, s. sanguis, s. salivarius, s. mitior, s. milleri, s. faecalis, s. faecium, s. avium, lactobacillus casei and l. salivarius. the polyols were added to a final concentration of 1.0% to two different basal media. incubation was performed at 37 degrees c for 7 days after which the ph was ...197721508
antimicrobial activity of human ascitic fluid. 197719339
plaque formation by streptococci in an artificial mouth and factors influencing colonization. 197718435
serological grouping of streptococci by a slide coagglutination method.a new method for the serological grouping of streptococci by coagglutination with specific antibodies absorbed to protein a-containing staphylococci has been assessed. a total of 242 strains of streptococci, including beta-haemolytic streptococci of groups a, b, c, f, and g, streptococcus pneumoniae and strep. faecalis were studied. all streptococci of groups a, b, c, and g, groupable by standard methods, were correctly grouped by coagglutination, although 7-3% showed varying degrees of cross-a ...197714975
[index of contamination of fecal origin in mussels (mytilus edulis) without purification of the ria de arosa]. 197797772
interactions of helium, oxygen, and nitrous oxide affecting bacterial growth.helium at pressures of 20 to 70 atm in the presence of air found to stimulate growth of streptococcus faecalis, escherichia coli, and staphylococcus aureus, mainly by increasing the rate of exponential growth. however, at these same pressures, helium potentiated the growth-inhibitory actions of oxygen and nitrous oxide (n2o). oxygen was found to act essentially as an anesthetic gas in inhibiting growth of s. faecalis; its potency was approximately the same as that of n2o, and it acted additively ...197897828
transport and metabolism of vitamin b6 in lactic acid bacteria.streptococcus faecalis 8043 concentrates extracellular 3hpyridoxal or 3hpyridoxamine primarily as the corresponding 5'-phosphates. accumulation of pyridoxamine requires an exogenous energy source and is inhibited by glycolysis inhibitors. a membrane potential is not required for transport of pyridoxamine, and an artificially generated potential does not drive uptake in this organism. based on this and other evidence, it is concluded that s. faecalis accumulates pyridoxamine by facilitated diffus ...197714135
ultrastructural, physiological, and cytochemical characterization of cores in group d streptococci.cores are large, rod-shaped structures that have been found almost exclusively in group d streptococci, measure 0.1 to 0.16 mum in diameter, and extend the width or length of cells. this study has shown that cores are produced in the cells at a reproducible point in early stationary growth after extensive mesosomal formation and after the ph has dropped below 6.5. when cells containing cores were introduced into a fresh medium with a ph above 6.5, the structures disappeared within 5 min. the str ...197712140
an improved method for determination of vitamin k. 19769016
the cleaning and disinfection by heat of bedpans in automatic and semi-automatic machines.this work is concerned with the cleaning and disinfection by heat of stainless-steel and polypropylene bedpans, which had been soiled with either a biological contaminant, human serum albumin (hsa) labelled with technetium-99m 99m(tc), or a bacteriological contaminant, streptococcus faecalis mixed with tc-labelled hsa. results of cleaning and disinfection achieved with a test machine and those achieved by procedures adopted in eight different wards of a general hospital are reported. bedpan wash ...19766591
microbial interactions in foods: meats, poultry and dairy products. 19765780
enterococcal survival in forcemeat preserved in polymer films and in cutlets made from it.the available data suggest that at 2 degrees and 5 degrees with a 12-day long storage in unpacked and in polymeric film packed forcemeat there occurs no propagation of the enterococci. at 22-24 degrees the multiplication of str. faecalis var. liquefaciens proceeded similarly both in packed and unpacked forcemeat. when the temperature in the central part of the cutlets prepared from the enterococci-contaminated forcemeat reaches 7-80 degrees the bulk of the str. faecalis var liquefaciens cells pe ...19751906
accumulation of thallous ions (tl+) as a measure of the electrical potential difference across the cytoplasmic membrane of bacteria.the accumulation of thallous ions (204tl+) by intact bacteria was investigated. i conclude that tl+ is a permeant cation, and that it therefore accumulates in response to the electrical potential difference (delta psi) across the cytoplasmic membrane (interior negative). a comparison with other methods shows that the distribution ratio of 204tl+ serves as a reasonably satisfactory method for measuring the membrane potential of streptococcus faecalis. glycolyzing cells of this organism develop me ...197899163
culture-negative endocarditis. 197874643
antagonistic interaction of rifampicin and trimethoprim. 197899420
casein degradation and amino acid liberation in milk by two highly proteolytic strains of lactic acid bacteria.amongst 100 isolates of lactic acid bacteria, lactobacillus bulgaricus and streptococcus feacalis proved to be highly proteolytic as demonstrated by starch gel electrophoresis and liberation of various amino acids. both these organisms hydrolysed casein differently to produce different concentrations of amino acids.197770973
circulation of h+ and k+ across the plasma membrane is not obligatory for bacterial growth.streptococcus faecalis grows normally in the presence of gramicidin and other ionophores under conditions such that there is no gradient of ph or of electrical potential across the plasma membrane and that currents of h+, k+, and na+ are short-circuited. growth requires a rich medium, a slightly alkaline ph, and a high concentration of external k+. the proton circulation maintains the cytoplasmic ph and pools of ions and other metabolites but is not obligatory for biosynthetic functions includin ...197769317
aspartate carbamyltransferase (streptococcus faecalis). 197899635
rapid identification and antibiotic sensitivity testing of bacteria isolated from clinical infections.a total of 834 bacterial strains isolated from urine were subjected to rapid biochemical and serological identification and rapid antimicrobial sensitivity testing using autobac 1. for enterobacteria (742 strains) six tests (acetoin-, beta-galactosidase-, hydrogensulphide-, indole-, ornithin-decarboxylase-and urease-production) correctly identified to genus or species level more than 99% of the strains within four hours. staphylococci and streptococci (92 strains) were identified with full accur ...197769255
effect of combinations of penicillin and aminoglycosides on streptococcus faecalis: a comparative study of seven aminoglycoside antibiotics.the 50% inhibitory concentrations (ic50) of benzylpenicillin, streptomycin, sisomicin, gentamicin, tobramycin, kanamycin, amikacin, and butirosin were determined for 58 clinical isolates of streptococcus faecalis, 28 of which were recovered from cultures of blood samples from patients with endocarditis. the ic50 of streptomycin was less than 100 microng/ml for 42 strains, 192-10,000 microng/ml for eight, and larger than or equal to 10,000 micron/ml for eight. one isolate that was highly resistan ...197767159
[the antibacterial activity of diphenhydramine (author's transl)].diphenhydramine (n,n-dimethyl-2-diphenyl-methoxy-ethylamine) [n,n-dimethyl-2-(diphenylmethoxy)-ethylamin] (dph) is a well-known local anaesthetic and an antagonist of both acetylcholine and histamine. we tested its antibacterial efficacy by means of three different investigation methods, i.e. microcalorimetry, continuous density measurement and the microdilution test to find out the mic of this compound against various bacteria. dph inhibits the growth of e. coli and klebsiella strains at a conc ...197899881
assessment of wound contamination by wound irrigation. experimental investigations on quantitative recovery of anaerobic and aerobic bacteria.the quantitative recovery of e. coli, s. faecalis and b. fragilis from operative abdominal wounds was investigated in pigs in an experimental model suitable for statistical calculations. wounds were contaminated in groups of ten with different numbers of either a single bacterial species or a mixture of two species. the wound was irrigated with saline 20 minutes after contamination. significant differences in recovery were found between the bacterial species investigated. expressed as percentage ...197899976
experimental endocarditis in rabbits. 2. course of untreated streptococcus faecalis infection.insertion of a polyethylene catheter in the left side of the heart was used to induce sterile endocarditis in 34 rabbits. bacterial endocarditis was established by injection of approximately 10(8) streptococcus faecalis into the blood stream simultaneous with the removal of the catheter which had been in place for 3 days. the course of the bacterial endocarditis was examined by autopsy of rabbits sacrificed at regular intervals after the infection. the results showed that the presence of the cat ...197899977
group g streptococcal endocarditis.the group g streptococcus may be a more common human pathogen than previously recognized. a case of group g streptococcal endocarditis is reported and the 11 cases reported previously are reviewed. group g endocarditis may have significant clinical and prognostic differences from endocarditis caused by the more commonly identified viridans or group d streptococci. routine serologic grouping of beta-hemolytic streptococcal isolates from serious infections is warranted.1978100003
effects of dispersed recreational activities on the microbiological quality of forest surface water.the microbiological quality of forest surface waters in the greenwater river watershed was examined to investigate the influence of heavy motorized camping in an area with no sanitary facilities. indicator densities increased during weekend human-use periods when compared to weekdays. increases in indicator densities were also noted downstream from heavily used camping areas when compared to upstream sites. seasonal, weekly, and diurnal fluctuations in indicator densities were observed. this stu ...1978100055
[immunoluminescent method for detecting enterococci in dairy products]. 1978100374
stomach as source of bacteria colonising respiratory tract during artificial ventilation.10 adults had intermittent positive-pressure ventilation for 3--34 days. all 10 had paralytic ileus. microbial overgrowth in the stomach was found in 9 patients (bacterial in 7 and fungal in 2); gram-negative bacteria predominated. the trachea invariably became colonised by bacteria, mainly gram-negative organisms. in 3 instances the gram-negative bacteria were found in the stomach before they appeared in the tracheal aspirate, and in 1 case the pathogen originated in the faeces.197881991
a new hydrolase specific for taurine-conjugates of bile acids.through the investigation of the bile acid-deconjugation activities of human intestinal anaerobes, a new enzyme was discovered in peptostreptococcus intermedius which hydrolyzed specifically the taurine-conjugates, but not the glycine-conjugates of bile acids. however, the enzymes in streptococcus faecalis and lactobacillus brevis hydrolyzed chiefly the glycine-conjugates.1978100491
[method of bacteriocin typing]. 197882670
laser differential light scattering bioassays for selected antitumor agents.bioassay methods for actinomycin d, 6-thioguanine, and adriamycin which utilize laser light scattering from suspensions of drug-sensitive bacteria have been developed. in 3 hours, serum and urine levels of the 3 drugs can be reproducibily measured. a sample volume of 0.1 ml may be assayed with a precision of +/- 10%. the linear response range for actinomycin d was 0.03 to 3.0 microgram/ml. for 6-thioguanine and adriamycin the linear responses range was 0.03 to 10.0 and 0.25 to 10.0 microgram/ml, ...1978100836
a rapid bioassay method for gramicidin by measuring rubidium ion leakage from streptococcus faecalis. 197991602
enterococcal endocarditis: duration and mode of treatment.this report summarizes data on sixteen patients with enterococcal endocarditis treated with penicillin and streptomycin. the experience reported suggests that a four week period is adequate for routine therapy in these patients, as in other forms of streptococcal endocarditis. it provides an additional group of patients successfully treated with penicillin and streptomycin. two relapses were encountered. one of these received inadequate daily doses of penicillin. the other patient was clearly a ...1978100924
relationship between cellular autolytic activity, peptidoglycan synthesis, septation, and the cell cycle in synchronized populations of streptococcus faecium.synchronized, slowly growing (td = 70 to 80 min) cultures were used to study several wall-associated parameters during the cell cycle: rate of peptidoglycan synthesis, septation, and cellular autolytic activity. the rate of peptidoglycan synthesis per cell declined during most of the period of chromosome replication (c), but increased during the latter part of c and into the period between chromosome termination and cell division (d). an increase in cellular septation was correlated with the inc ...197896095
[urobilinogen excretion in the urine and bacterial overgrowth in the jejunum of patients with jejuno-or ileo-transversostomy in man (author's transl)]. 1978101703
[prevention of bacterial endocarditis]. 1978101706
experimental renal infection: acute and chronic studies of histology and function.experimental renal infections have resulted in histologic lesions of acute and chronic pyelonephritis, but in most studies the urinary tract has been "traumatized" to induce persistent infection. sequential functional alterations have been measured infrequently. the present studies were designed to separate the functional and histological changes caused by high pressure sterile reflux from reflux with pathogenic organisms. reproducible disruption of the pelvic epithelium with extravasation of so ...1978101708
studies on virus-inhibiting substances of bacterial origin. iv. antiviral substances extractable from streptococcus faecalis: its in vivo effect. 1978101714
studies on virus-inhibiting substances of bacterial origin. i. introduction. 1978101715
studies on virus-inhibiting substances of bacterial origin. ii. inhibition of animal virus plaque formation by culture filtrates of lactic acid bacteria. 1978101716
approximation of the cell cycle in synchronized populations of streptococcus faecium.slowly growing populations (td = 70 to 80 min) of streptococcus faecium (s. faecalis atcc 9790) were synchronized by selection after sucrose gradient fractionation. the cell cycle was approximated by correlating the patterns of dna accumulation and cell division. more specifically, the beginning of cell cycle was equated with the beginning of a rapid linear increase in dna accumulation. the dna content of the culture approximately doubled during the period of accumulation, which lasted about 51 ...197896099
characterization of bacterial l-(-)-tyrosine decarboxylase by isoelectric focusing and gel chromatography.the purification of l-(-)-tyrosine apodecarboxylase (tad) (e.c. 4.1.1.25), obtained from extracts of cells of streptococcus faecalis, has been investigated by means of preparative isoelectric focusing, molecular sieve chromatography and hydrophobic interaction chromatography. isoelectric focusing demonstrated two separate fractions possessing enzyme activity that had pi values of 4.5 and ca. 3.2. in the chromatographic methods, however, the activity was obtained in a single peak. it was found th ...197896122
[the etiology of foul brood].five hundred and thirty-six samples of honeycombs were examined in a laboratory in the years 1971-1974. in all the samples clinically determined as the foul brood, b. alvei was isolated as a pure culture, and enterococci, or both microorganisms were isolated in mixed form. twenty-five strains of the isolated streptococci were analyzed microbiologically and biochemically; on the basis of their culture and biochemical characteristics five strains were designated as streptococcus faecalis, 14 strai ...1978102071
multivalvular vegetations in a patient with enterococcal endocarditis diagnosed by echocardiography.a patient with enterococcal endocarditis of 11 months' duration is presented, and the role of surgery and echocardiography is reviewed. echocardiography revealed vegetations of the aortic and mitral valves. after appropriate antibiotic therapy the patient had successful aortic and mitral valve replacement with porcine heterografts. enterococcal endocarditis is increasing in frequency and is likely to infect young women of childbearing age, elderly men who have had genitourinary tract manipulatio ...197896535
plasmid-mediated mechanisms of resistance to aminoglycoside-aminocyclitol antibiotics and to chloramphenicol in group d streptococci.genes conferring resistance to aminoglycoside-aminocyclitol antibiotics in three group d streptococcal strains, streptococcus faecalis jh1 and jh6 and s. faecium jh7, and to chloramphenicol in jh6 are carried by plasmids that can transfer to other s. faecalis cells. the aminoglycoside resistance is mediated by constitutively synthesized phosphotransferase enzymes that have substrate profiles very similar to those of aminoglycoside phosphotransferases found in gram-negative bacteria. phosphorylat ...197896732
folate antagonists. 13. 2,4-diamino-6-](alpha,alpha,alpha-trifluoro-m-tolyl)thio]quinazoline and related 2,4-diamino-6-[(phenyl- and naphthyl)thio]quinazolines, a unique class of antimetabolites with extraordinary antimalarial and antibacterial effects.an array of nonclassical thioquinazoline analogues (viii) of methotrexate was prepared by cyclization of the requisite 2-amino-5-(arylthio)benzonitrile with chloroformamidine hydrochloride (28--79%). the aminonitrile precursors were obtained by sncl2-hcl reduction (28--99%) of the corresponding 2-nitro-5-(arylthio)benzonitriles, which were synthesized by the condensation of the appropriate 5-chloro-2-nitrobenzonitriles with various arylthiols (36--83%). many of the thioquinazolines (viii) showed ...1978102792
aminoglycoside-inactivating enzymes in clinical isolates of streptococcus faecalis. an explanation for resistance to antibiotic synergism.clinical isolates of enterococci (streptococcus faecalis) with high-level resistance to both streptomycin and kanamycin (minimal inhibitory concentration >2,000 mug/ml), and resistant to synergism with penicillin and streptomycin or kanamycin were examined for aminoglycoside-inactivating enzymes. all of the 10 strains studied had streptomycin adenylyltransferase and neomycin phosphotransferase activities; the latter enzyme phosphorylated amikacin as well as its normal substrates, such as kanamyc ...197897312
damage and recovery in streptococci. 1978103202
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