| chimeric streptococcal plasmids and their use as molecular cloning vehicles in streptococcus sanguis (challis). | chimeric plasmids, which were useful as cloning vehicles in a streptococcus sanguis (challis) host vector system, have been constructed. by using three different strategies of restriction endonuclease digestion and ligation, a deoxyribonucleic acid (dna) fragment bearing an erythromycin resistance determinant was ligated in vitro to a phenotypially cryptic plasmid from streptococcus ferus. recombinant plasmids could be recovered after transformation of s. sanguis (challis) with these preparation ... | 1980 | 6251030 |
| physiology of "mutans-like" streptococcus ferus from wild rats. | strains of streptococcus ferus isolated from the oral cavities of wild rodents inhabiting sucrose-rich and sucrose-poor environments have many traits in common with the "mutans" streptococci. thus, s. ferus hd3 and 8s1, like cariogenic s. sobrinus 6715-13, from adherent, alpha (1 leads to 3) glucopyranosyl-glucose linkage-rich, plaquelike deposits in vitro and in vivo through the action of constitutive glucosyltransferase(s) enzymes on sucrose, produce and degrade intracellular polysaccharide, p ... | 1982 | 6276304 |
| penicillin-induced lysis of streptococcus mutans. | treatment of streptococcus mutans gs-5 cells with concentrations of penicillin g within a relatively narrow range resulted in substantial lysis. this penicillin-induced lysis was dependent upon cell density and ph of the lysis medium. other oral streptococci (streptococcus sobrinus, streptococcus rattus, and streptococcus cricetus) also demonstrated substantial levels of penicillin-induced lysis under appropriate conditions. lesser degrees of lysis were seen in a related organism, streptococcus ... | 1984 | 6500698 |
| glycerol incorporation in certain oral streptococci. | cells of 30 different strains of oral streptococci were grown in a chemically defined medium supplemented with [14c]glycerol to determine their ability to incorporate the labeled glycerol. of the five species tested, only two, the rat-type strains (streptococcus rattus) and strains isolated from wild rats (streptococcus ferus), were able to incorporate the nonfermentable substrate, glycerol. for those strains capable of incorporating glycerol, the amount incorporated ranged from 0.15 to 0.43% of ... | 1980 | 7014448 |
| colonization and cariogenicity of streptococcus ferus in rats. | streptococcus ferus, which is indigenous to wild rats, is a member of the mutans group of streptococci. we tested its ability to colonize and to cause caries in laboratory rats by comparing two strains of s. ferus with the very cariogenic streptococcus sobrinus strain 6715. groups of rats were fed either finely ground mouse chow or a 56% sucrose diet, or they were switched from chow to the sucrose diet. all three strains colonized the mouths of rats regardless of diet. however, the infectants re ... | 1981 | 7216496 |
| genetic transformation of streptococcus sanguis (challis) with cryptic plasmids from streptococcus ferus. | by using the basic methodology initially published by kretschmer et al. (j. bacteriol. 124:225-231, 1975), we have been able to introduce phenotypically cryptic plasmids from streptococcus ferus (formerly streptococcus mutans subsp. ferus) into streptococcus sanguis by genetic transformation. in this system, the entry of the cryptic plasmids is selected indirectly. this is effected with transforming deoxyribonucleic acid mixtures in which the cryptic plasmid deoxyribonucleic acid is present in a ... | 1980 | 7399689 |
| mobilisation of the streptococcal plasmid pmv158: interactions of mobm protein with its cognate orit dna region. | the streptococcal plasmid pmv158 encodes the relaxase protein, mobm, involved in its mobilisation. purified mobm protein specifically cleaved supercoiled or single-stranded dna containing the plasmid origin of transfer, orit. gel retardation and dnase i footprinting assays performed with dna fragments containing the plasmid orit provided evidence for specific binding of mobm by orit dna. dissection of the mobm-binding sequence revealed that the orit region protected by mobm spanned 28 nucleotide ... | 1999 | 10394908 |
| re-evaluation of the taxonomic position of streptococcus ferus. | streptococcus ferus was originally isolated from rats and, although it is often considered a member of the 'mutans group' streptococci on the basis of phenotypic criteria, its phylogenetic position has not been investigated. here, the relationship of this organism to other streptococcal groups has been determined by dna-dna hybridization and by partial sequencing of 16s rdna and the soda gene. s. ferus was found to be distantly related to all currently described streptococci, with no strong evid ... | 2002 | 12361287 |
| emended description of streptococcus ferus isolated from pigs and rats. | streptococcus ferus is a mutans-like streptococcus originally isolated from wild rats fed with sugar cane. taxonomically, this species has not been studied extensively. ten gram-positive coccal strains, isolated from the tonsils and nasal conchae of piglets, were identified as s. ferus by 16s rdna sequencing, trna-intergenic spacer length polymorphism analysis (tdna-pcr), whole-cell protein profiling using sds-page, g+c content determination and extensive biochemical testing. in all these tests, ... | 2003 | 12656166 |
| identification of mutans streptococci by restriction fragment length polymorphism analysis of polymerase chain reaction-amplified 16s ribosomal rna genes. | mutans streptococci are frequently isolated from dental plaque and carious lesions. these bacteria have been identified by conventional methods such as biochemical and serologic tests followed by the isolation of colonies on the mitis-salivarius agar, which are sometimes inconsistent. recently, species-specific polymerase chain reaction (pcr) has been reported to rapidly identify streptococcus mutans and streptococcus sobrinus. however, in the case of identification and classification into sever ... | 2003 | 12930526 |
| streptococcus devriesei sp. nov., from equine teeth. | phenotypic and phylogenetic studies were performed on four unidentified gram-positive staining, catalase-negative, alpha-hemolytic streptococcus-like organisms recovered from the teeth of horses. sds page analysis of whole-cell proteins and comparative 16s rrna gene sequencing demonstrated the four strains were highly related to each other but that they did not correspond to any recognised species of the genus streptococcus. phylogenetic analysis based on 16s rrna gene sequences showed the unide ... | 2004 | 15046302 |
| streptococcus massiliensis sp. nov., isolated from a patient blood culture. | an unidentified strain of the viridans group of streptococci was isolated from a human blood sample. it was distinguished from all other recognized species of the streptococcus sanguinis group by several biochemical characteristics. phylogenetic analysis based on 16s rrna gene sequence comparisons clustered this strain with streptococcus ferus (mutans group) but phylogenetic analysis based on rpob and soda gene sequence comparisons included it in the s. sanguinis group. the isolate showed 95.4 a ... | 2006 | 16627666 |
| comparison of culture media and chairside assays for enumerating mutans streptococci. | this study compared several traditional culture-based media and chairside cultural assays for ability to recover mutans streptococci (ms) from pure cultures and from saliva samples. | 2006 | 16696682 |
| bioactive steroidal alkaloids from sarcococca hookeriana. | four new 5 alpha-pregnane-type steroidal alkaloids, hookerianamides l(1), m(2), n(3), and o(4), and a known n-formylchonemorphine (5) have been isolated by acid-base extraction of the dichloromethane extract of sarcococca hookeriana. the structures of all compounds were determined with spectroscopic techniques and by comparison with literature data. all compounds displayed antileishmanial and antibacterial properties. compounds 1, 4, and 5 were found to be more potent than standard pentamidine ( ... | 2010 | 20186658 |