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evidence for the synthesis and release of strongly immunosuppressive, noncytotoxic substances by streptococcus intermedius.products secreted by streptococcus intermedius were studied for their effects on the immune response. three different preparations of crude extracellular products from s. intermedius (cep-si) were found to have powerful suppressor activity in vitro as shown by inhibition of human lymphocyte proliferation (uptake of [3h]thymidine) and protein synthesis in response to a wide variety of stimulants, including mitogens and antigens, and suppression of plaque formation by human cells in response to sh ...1979383749
serologic studies of streptococcus intermedius, streptococcus constellatus, and streptococcus morbillorum by crossed immunoelectrophoresis.a reference antigen-antibody system for streptococcus intermedius, streptococcus constellatus, and streptococcus morbillorum was established with crossed immunoelectrophoresis. a comparison of s. intermedius, s. constellatus, and s. morbillorum with crossed immunoelectrophoresis and crossed immunoelectrophoresis with intermediate gel indicated that s. intermedius and s. constellatus are closely related antigenically with as many as six common cytoplasmic antigens. s. morbillorum was antigenicall ...1979453098
survival of anaerobic bacteria in common laboratory diluents.the survival of six species of anaerobic bacteria was studied in simple or commercially available diluents. bacteroides fragilis and fusobacterium nucleatum showed excellent survival in all diluents including distilled water. fusobacterium mortiferum survived well in all diluents except water and water supplemented with 0.1% gelatain. clostridium perfringens survived best in phosphate-buffered saline with gelatin. peptococcus asaccharolyticus required gelatin added to the basic diluent, and stre ...1979479359
differentiation of peptococcus and peptostreptococcus by gas-liquid chromatography of cellular fatty acids and metabolic products.gas-liquid chromatographic (glc) profiles of cellular fatty acids and metabolic products were useful in identifying strains of peptococcus saccharolyticus, peptococcus asaccharolyticus, peptostreptococcus anaerobius, peptostreptococcus micros, and streptococcus intermedius. the glc results supported the recent taxonomic decision to transfer aerotolerant peptostreptococcus species to the genus streptococcus. because inconsistencies in the results prevented our differentiating peptococcus prevotii ...1979528680
inhibitory effects of extracellular products from oral bacteria on human fibroblasts and stimulated lymphocytes.extracellular products of 12 strains of streptococcus mutans and 5 additional species of oral bacteria were analyzed for their ability to inhibit proliferation of fibroblastoid cells (hela and av3) and blast transformation of human peripheral blood lymphocytes obtained from normal individuals. products from s. mutans strains aht and bht, streptococcus intermedius, and actinomyces viscosus inhibited [3h]thymidine uptake by fibroblastoid cells and phytohemagglutinin-stimulated lymphocytes. product ...1978689736
invasion of streptococcus mutans, streptococcus intermedius and propionibacterium acnes into the teeth of gnotobiotic rats.germ-free rats, fed a modified cariogenic diet 2000 with a reduced sucrose content of 25%, were inoculated with a strain of streptococcus mutans, streptococcus intermedius or propionibacterium acnes, respectively. after 100 days on the diet, the rats were sacrificed. microscopic examination of stained decalcified tooth sections revealed marked destruction of the outer zones of the dentin infected with s. mutans. the destruction was closely associated with the formation of glucan-mediated large a ...19921325873
hepatic pathology of chronic granulomatous disease of childhood.we reviewed the hepatic pathology of seven cases of chronic granulomatous disease of childhood. all patients were male, with an age range of 5 to 41 years. hepatic biopsy with drainage or wedge resection was performed in five cases to remove abscesses. autopsy was performed in three cases. presentation was typical of infection (fever, leukocytosis) with an elevated serum alkaline phosphatase level. histologically, the most consistent feature was the presence of foamy macrophages that contained a ...19921370879
protective effect of a t-cell-dependent immunosuppressive, b-cell-mitogenic protein (f3'ep-si, or p90) produced by streptococcus intermedius.the role of a previously described bacterial protein (f3'ep-si), now designated p90, in the survival of streptococcus intermedius in the host was investigated, and the immunosuppressive and b-cell-mitogenic effects of this protein were further characterized. c57bl6 mice treated with p90 were about 50 times more susceptible to infection with this bacterium than untreated mice. one of seven splenocytes of c57bl/6 mice were activated by p90. marked splenomegaly was observed in mice treated with p90 ...19921500165
streptococcus milleri group: renewed interest in an elusive pathogen.the following review examines the bacteriological characteristics, epidemiology, pathogenicity and antimicrobial susceptibility of the "streptococcus milleri group". "streptococcus milleri group" is a term for a large group of streptococci which includes streptococcus intermedius, streptococcus constellatus and streptococcus anginosus. usually considered commensals, these organisms are often associated with various pyogenic infections including cardiac, abdominal, skin and central nervous system ...19921526231
streptococcus intermedius, streptococcus constellatus, and streptococcus anginosus (the streptococcus milleri group): association with different body sites and clinical infections.the associations of streptococcus intermedius, s. constellatus, and s. anginosus (the three species of the s. milleri group) with clinical infections and sites of isolation were investigated by using a simple biochemical scheme to identify a collection of 153 clinical isolates. s. intermedius was associated with abscesses of the brain and liver, while both s. anginosus and s. constellatus were isolated from a wider range of sites and infections. s. anginosus strains predominated in both genitour ...19921734062
thoracic empyema due to streptococcus intermedius.with improved laboratory identification procedures, streptococcus milleri (intermedius) is becoming recognized as an important human pathogen with the potential to form abscesses in a wide range of organs. the occurrence of a rapidly progressive and toxic empyema due to this organism in an otherwise healthy young adult without a predisposing lower respiratory tract infection allows us to examine the possibility that this microbe may be a significant but frequently unrecognized cause of adult emp ...19911748832
[studies on clinical significance of "streptococcus milleri group" in respiratory infections]."streptococcus milleri group" are the part of the indigenous oral flora, and they are proposed to contain three distinct species: streptococcus anginosus, streptococcus constellatus, and streptococcus intermedius. though not included in the approved lists of bacterial names, "s. milleri group" are regarded as the causative organisms of suppurative infections, such as oral abscess, brain abscess, lung abscess and empyema. i have studied the clinical significance of the "s. milleri group" in respi ...19911791343
[purulent meningitis caused by streptococcus intermedius (s. milleri) related to an otogenic focus]. 19911932257
emended descriptions and recognition of streptococcus constellatus, streptococcus intermedius, and streptococcus anginosus as distinct species.strains currently classified as streptococcus anginosus include strains previously identified as streptococcus constellatus (prevot 1924) holdeman and moore 1974, streptococcus intermedius (prevot 1925), and "streptococcus milleri" (guthof 1956) because these specific epithets were argued to be later synonyms of streptococcus anginosus (andrewes and horder 1906) smith and sherman 1938 by coykendall et al. (int. j. syst. bacteriol. 37:222-228, 1987). however, recent data from dna-dna hybridizatio ...19911995029
clinical, microbiological and immunological characteristics of subjects with "refractory" periodontal disease.the aim of the present study was to analyze the relationship between attachment loss and clinical, microbiological, and immunological parameters in a group of 21 human subjects exhibiting poor response to previous periodontal therapy. all had been treated with periodontal surgery, tetracycline, and subsequent maintenance recalls to periodontists who, upon detection of disease progression, referred the subjects to our clinic. in our clinic, each subject received oral hygiene instruction and a tho ...19912066442
[anaerobic bacteria detected in inflammatory conditions of the respiratory tract].in this study a participation of anaerobic bacteria in respiratory tract diseases is presented. bronchial washings collected by ++fibrobronchoscope constituted material for the study. immediately after collection the material was plated onto two media for aerobic bacteria (hemomedium) and anaerobic bacteria (anaeromedium). then, the samples were centrifuged and a sediment was plated on solid media suitable for aerobic and anaerobic bacteria. bacterial anaerobic isolates were identified by using ...19902084446
serogrouping of oral streptococcus intermedius.employing twenty fresh oral isolates of streptococcus intermedius, studies were carried out to characterize serological relations among the isolates and also between the isolates and the strains of bacterial species closely related to s. intermedius. the rantz-randall extracts from the cells were used as antigens. the anti-rabbit serum raised against s. intermedius atcc 27335t reacted with the cell extracts from only three strains of the isolates, which were designated serogroup i strains. the o ...19902111871
genetic and physiological characteristics of oral streptococcus intermedius.the taxonomic relationships among strains of oral streptococcus intermedius and the related streptococci were determined by g + c mol%, dna-dna hydridization, phenotypical and serological tests. as to the results, group 1 strains of s. intermedius including the type strain atcc 27335 and five isolates behaved distinctively with respect to genetic, phenotypic and serological characteristics compared with group 2 strains of s. intermedius including atcc 31412 and two isolates. therefore, nine stra ...19902130861
a rapid diagnosis of anaerobic infection in the oro-maxillary region by gas-liquid chromatography.the relationship between volatile fatty acids (vfas) in pus and infecting bacterial species was examined in order to establish a rapid identification system for anaerobic microorganisms in purulent inflammation in the oro-maxillary region. vfas were detected by the direct injection of pus into gas-liquid chromatography (glc). bacterial examination was carried out by anaerobic culture using blood agar plates. the bacterial identification was carried out mainly according to the vpi manual. analysi ...19902131168
[eikenella corrodens as part of the polymicrobial flora in infectious endocarditis].the authors describe a case of polymicrobial endocarditis in a 24-year-old patient with immunodeficiency and a damaged heart (mitral valve affection, septal defect). among the mixed flora, comprising streptococcus intermedius and two types of the genus bacteroides, in the haemoculture also a microaerophil potentially pathogenic type eikenella corrodens was cultivated. the authors describe the properties of eikenella corrodens, its occurrence in man and its ability to produce an infectious proces ...19902150617
eikenella corrodens infection of the oral cavity as a cause of bacterial endocarditis.the authors describe the case of a 24-year-old woman with valve disease. after a bout of respiratory tract infection, she was diagnosed to have bacterial endocarditis associated with mitral valve disease. the causative agents isolated included eikenella corrodens, streptococcus intermedius, bacteroides oralis and bacteroides bivius. at the same time, the patient was found to have developed iga immunodeficiency. a complication accompanying the cardiac disease was spontaneous pneumothorax. since a ...19902190761
recolonization of the subgingival microflora after scaling and root planing in human periodontitis.the purpose of this study was to evaluate the recolonization patterns of the subgingival microflora of adult periodontitis patients after a single session of scaling and root planing. in each of eight patients, three clinically diseased sites were investigated microbiologically by darkfield microscopy and cultural analysis. after initial clinical and microbiological parameters were determined, each subject received a single session of scaling and root planing but no oral hygiene instructions. cl ...19902213468
species-specific oligodeoxynucleotide probes for the identification of periodontal bacteria.oligodeoxynucleotide probes were developed for identification of the periodontal bacteria actinobacillus actinomycetemcomitans, bacteroides gingivalis, b. intermedius types i and ii, b. forsythus, eikenella corrodens, fusobacterium nucleatum, haemophilus aphrophilus, streptococcus intermedius, and wolinella recta. probes were designed by sequencing the 16s rrna for each bacterium, identifying hypervariable regions, and chemically synthesizing species-specific probes. these probes were specific w ...19902312676
multiple periodontal abscesses after systemic antibiotic therapy.multiple periodontal abscesses were reported in medically compromised patients. we examined patients with a non-contributory medical history referred for the treatment of numerous periodontal abscesses. all patients had taken oral broad spectrum antibiotics 1 to 3 weeks prior to the outburst of the abscesses (8 patients: penicillin, 2 patients: tetracycline). the patients suffered from advanced periodontal disease, 82% of the examined sites showed probing depths greater than 3 mm, 56% attachment ...19902347952
phenotypic differentiation of streptococcus intermedius, streptococcus constellatus, and streptococcus anginosus strains within the "streptococcus milleri group".a biochemical scheme was developed by which strains of streptococcus constellatus, streptococcus intermedius, and streptococcus anginosus can reliably be distinguished from within the "streptococcus milleri group." strains identified as s. intermedius were differentiated by the ability to produce detectable levels of alpha-glucosidase, beta-galactosidase, beta-d-fucosidase, beta-n-acetylgalactosaminidase, beta-n-acetylglucosaminidase, and sialidase with 4-methylumbelliferyl-linked fluorogenic su ...19902380375
association of viridans group streptococci from pregnant women with bacterial vaginosis and upper genital tract infection.the prevalence and role of viridans group streptococci in the female genital tract have not been well described. in this study of 482 pregnant women, 147 (30%) were culture positive for viridans group streptococci. of 392 women with predominant lactobacillus morphotypes by gram stain (normal), 110 (28%) were colonized with viridans group streptococci, compared with 37 (41%) of 90 women with bacterial vaginosis (bv) (p = 0.02). to determine whether any species were associated with bv, 177 consecu ...19882454943
[anaerobic bacteria in intracranial suppuration].in four cases of intracranial suppurative disease, studied in the laboratory of anaerobic bacteria of institute pasteur of algiers, three cases are caused by anaerobic bacteria and one remain sterile. two of them are empyema with unknown origin and the others are brain abscess with bilateral chronic ear infection to one. in the three cases, gram negative anaerobic bacilli were grown in culture: bacteroides asaccharolyticus, bacteroides vulgatus and bacteroides ruminicola brevis. in two cases, th ...19892489404
chondroitinase c activity of streptococcus intermedius.chondroitin 6-sulfate depolymerizing activity was examined in the culture supernatant of streptococcus intermedius atcc 27335. 2-acetamido-2-deoxy-3-o-(beta-d-gluco-4-enepyranosyluronic acid)-6-o-sulfo-d-galactose was split from the substrate. the enzyme(s) was not active upon chondroitin 4-sulfate or dermatan sulfate, which indicated that the enzyme responsible for the depolymerization is chondroitinase c.19892498150
a qualitative and quantitative study of the cellular fatty acids of 'streptococcus milleri' with capillary gas chromatography.fatty acid analysis was done with gc and gc-ms on 21 strains of 'streptococcus milleri', representative of the various proposed species. although no qualitative differences were found in the fatty acid profiles, discriminant analysis of the quantitative data revealed three groups. streptococcus anginosus and streptococcus constellatus were indistinguishable but separated from the other two groups which comprised streptococcus intermedius, with a wide fermentation pattern and streptococcus interm ...19892600587
[taxonomic studies of the streptococcus intermedius strains isolated from human oral cavities].human oral isolates of streptococcus intermedius, which were biochemically identified, have been classified into 5-serogroups (i-v). selecting some representive stock cultures together with the type strain atcc 27335, comparisons were carried out between them in terms of cell extractable proteins, g + c mol% values, dna-dna homology and dna restriction fragments. since the taxon of s. intermedius has not been clarified, comparison was also made between the above strains and the type strains of t ...19892610299
dna-dna hybridization studies and phenotypic characteristics of strains within the 'streptococcus milleri group'.twenty-five strains resembling 'streptococcus milleri' were compared by dna-dna hybridization, by whole-cell-derived polypeptide patterns on sds-page, and by biochemical tests. four homology groups were revealed by dna-dna hybridization. dna homology groups 1, 2 and 3 were closely related and contained the type strains ncdo 2226 (streptococcus constellatus), ncdo 2227 (streptococcus intermedius) and nctc 10713 (streptococcus anginosus), respectively. dna homology group 4 consisted of four strain ...19892632666
[cariogenicity of propionibacterium acnes, streptococcus intermedius and streptococcus mutans in germ-free rats].a comparative study has been made on the cariogenic potentials of propionibacterium acnes atcc 11828, streptococcus intermedius oe-1 and streptococcus mutans oz-1. the strains of p. acnes and s. intermedius have chondroitinase activity. as metabolic end-products, the p. acnes strain produces mainly a weak acid, propionic acid, whereas strong lactic acid is the main product of the strains of s. intermedius and s. mutans. five germ-free rats were used for infection with each bacterial strain. the ...19892637631
[studies on anaerobic infection in oro-maxillary region--rapid diagnosis by gas-liquid chromatography and antibiotic susceptibilities of anaerobic bacteria].subject material for this study was pus collected from patients with purulent inflammation in the oro-maxillary region. direct gas-liquid chromatography (glc) analysis was made, bacterial isolation and identification were carried out, and comparisons were made with results from glc analysis and anaerobic isolates in a pyg medium. in addition, antibiotic susceptibilities of anaerobic bacteria were examined. results 1. anaerobic bacteria were isolated from 85 of 100 cases of obstructive abscesses. ...19892641196
ischaemic mouse thigh model for evaluation of pathogenicity of non-clostridial anaerobes.a mouse thigh model has been devised in which the growing culture of non-clostridial anaerobe in the ischaemic tissue produces inflammatory swelling and death. the swelling of the right thigh served as an index of pathogenicity of the test strain in comparison to the negatively reacting left thigh which received injection of the control strain of bifidobacterium infantis. actinomyces naeslundii exceptionally caused death in all animals within 24 h. mortality and thigh swelling were pronounced (g ...19892914729
[clinical infections due to streptococcus intermedius]. 19883212249
[localization of streptococcus intermedius in the sound teeth and gingival sulci]. 19883269197
does an intracervical infection influence the fibrinolytic activity and the collagen content of the fetal membranes? a study of ascending infections in pregnant ewes.apart from solely mechanical explanations, premature rupture of the membranes (prom) has been suggested to be caused by an ascending infection. in order to investigate the role of infection in the mechanism of prom, pregnant ewes were experimentally inoculated endocervically with either bacteroides fragilis, streptococcus intermedius or group b streptococci. these microorganisms were previously reported to be implicated in prom in humans. the present investigation concerns the possible effect of ...19873301451
serious infections due to penicillin-resistant strains of viridans streptococci with altered penicillin-binding proteins.we investigated the mechanism of resistance to penicillin in two penicillin-resistant clinical isolates of viridans streptococci that caused life-threatening infections in two patients not receiving chronic penicillin therapy. the first was a strain of streptococcus intermedius that was isolated from the cerebrospinal fluid of a patient with post-neurosurgical meningitis. the second was a strain of streptococcus mitis recovered from the bloodstream of a leukemic patient with neutropenia. both pa ...19883346567
the synergistic effect of cefotaxime and desacetylcefotaxime against clinical isolates of anaerobic bacteria.the synergistic interaction of cefotaxime and desacetylcefotaxime against 187 clinically significant anaerobic organisms was investigated. fusobacterium nucleatum, actinomyces odontolyticus, propionibacteria, lactobacilli, peptostreptococci, streptococcus intermedius and veillonella were sensitive to cefotaxime. both eubacterium lentum and streptococcus morbillorum were resistant. the susceptibility of the clostridia varied from 0.125 to greater than 256 mg/l; only 20% of species demonstrated sy ...19883396488
[effects of ceftriaxone on infections during the perinatal period].ceftriaxone (ctrx) was studied for its efficacy and safety in 8 cases of infection during the perinatal period; 6 before, and 2 after delivery. the results obtained are recognized as follows: 1. ctrx was administered by intravenous drip infusion at a daily dose between 2 and 4 g for 2 to 10 days (a total dose: 4 to 20 g) each of 8 cases of infections during the perinatal period; 3 of amniotic fluid infection and 1 each of intrauterine infection, puerperal fever, puerperal wound infection, append ...19883404648
chondroitin sulfate-depolymerizing activity in streptococcus intermedius and other streptococci.the type strain (atcc 27335) and 18 human oral isolates of streptococcus intermedius and some other related streptococcal species were tested for chondroitin sulfate c-depolymerizing activity employing a modified screening plate method of smith and willett. as the results, s. intermedius strains except for atcc 31412 strain were found to possess this activity. propionibacterium acnes atcc 11828 used as a positive control strain demonstrated strong activity, whereas s. intermedius strains showed ...19873444428
frequency distributions of periodontal attachment loss. clinical and microbiological features.the present investigation attempted to determine if the pattern of past periodontal destruction could be concisely summarized, and related to other clinical and microbiological parameters. 61 subjects between the ages of 12 and 61 years with destructive periodontal disease were evaluated at 6 sites per tooth for redness, plaque, suppuration, bleeding on probing, pocket depth, and attachment level. the frequency distribution of baseline attachment level measurements was computed for each individu ...19863462210
[grouping of oral streptococcus intermedius]. 19863466553
[cariogenicity of oral streptococcus intermedius in germ-free rats]. 19863484198
strong mitogenic effect for murine b lymphocytes of an immunosuppressor substance released by streptococcus intermedius.a noncytotoxic protein substance, produced by streptococcus intermedius, with very potent immunosuppressive properties (f3'ep-si) was tested for lymphocyte mitogenic activity. although devoid of t-cell mitogenicity, f3'ep-si stimulated proliferation and led to high numbers of plaque-forming cells in cultures of normal or t-cell-depleted, small or large splenic b cells from both lipopolysaccharide-responding and -nonresponding mice. the b-cell mitogenic activity of f3'ep-si was quantitatively com ...19863490441
mechanisms of successful amoxicillin prophylaxis of experimental endocarditis due to streptococcus intermedius.prophylaxis with amoxicillin (40 mg/kg) was studied in rats with aortic valve vegetations. bacteria on the valves were quantitated early (10 min to 6 hr) and late (three days) after intravenous challenge with tolerant streptococcus intermedius. amoxicillin reduced by 40% the number of bacteria per valve 10 min after intravenous challenge with 10(5) s. intermedius (p less than .05) and by 74% the incidence of endocarditis three days thereafter (p less than .0001). bacterial multiplication started ...19863490521
attachment level changes in destructive periodontal diseases.the present communication attempts to summarize some of the features of attachment loss which are of interest to the clinician and the statistician analyzing data from clinical trials. these include the measurements employed to detect changes in attachment level, the nature of the destructive disease process and the effects of therapy on the attachment level measurements. although there are several difficulties associated with the attachment level measurement, at the present time it appears to b ...19863522651
viridans streptococcal endocarditis: clinical, microbiological, and echocardiographic correlations.infections caused by species within the viridans streptococci have been associated with different clinical characteristics. we studied 36 patients with viridans streptococcal endocarditis. complications were seen in 10 (32%) of 31 patients with native valve endocarditis and four (80%) of five with prosthetic valve endocarditis and included death in two, valve replacement in six, persistent infection in three, emboli in two, and congestive heart failure in nine. two-dimensional echocardiograms de ...19863745973
[clinical studies on aztreonam in various infections in obstetrics and gynecology].aztreonam (azt) was administered to 10 patients with obstetric and gynecologic infections to evaluate its clinical effect. two-four grams of azt per day (b.i.d.) were administered for the treatment for 4-15 days by intravenous drip infusion or intravenous injection. azt was effective for 3 cases of pelvic peritonitis, 1 case of pyoovarium and 2 cases of bartholin's abscess. for 2 cases of intrauterine infection, azt was effective for 1 case, and the other case could not be judged. for 2 cases of ...19853834137
survival analysis of periodontal sites before and after periodontal therapy.periodontal diseases appear to progress with bursts of destructive activity at individual sites. one effect of treatment might be to diminish the frequency of such bursts. survival analysis was employed to seek such effects on the periodontal sites of 16 individuals with prior evidence of destructive periodontal disease. the subjects were monitored at bi-monthly intervals and actively breaking down sites were detected using attachment level measurements and the tolerance method of analysis. when ...19853860518
[in vitro cariogenicity of streptococcus intermedius and bifidobacterium]. 19853867293
[cariogenicity of streptococcus intermedius atcc 27335 in conventional hamsters and rats]. 19853867294
the relationships between streptococcal species and periodontopathic bacteria in human dental plaque.the existence of antagonistic and commensal relationships between microorganisms was investigated. the predominant cultivable flora in 172 plaque samples from active and non-active sites in 32 human subjects with destructive periodontitis was determined. the presence of putative periodontopathic organisms (bacteroides gingivalis, bacteroides intermedius, bacteroides forsythus, wolinella recta, actinobacillus actinomycetemcomitans, and eikenella corrodens) in a site was correlated with the absenc ...19853868968
agar medium for gas-liquid chromatography of anaerobes.this study evaluates a method of performing gas-liquid chromatography (glc) by direct extraction of fatty acids from agar for identification of clinically significant anaerobic bacteria. the potential use of agar cultures for glc was studied by comparing chromatograms of 117 clinically isolated anaerobes grown in peptone yeast glucose broth and chopped meat carbohydrate broth, and on enriched brucella blood agar. for 98 of 117 anaerobes, fatty acid patterns from agar cultures were similar to tho ...19863940426
successful prophylaxis of experimental streptococcal endocarditis with single doses of sublethal concentrations of penicillin.penicillin prophylaxis against experimental endocarditis due to a strain of streptococcus intermedius isolated from a patient with endocarditis was studied in rats. the minimum bactericidal concentration of penicillin for this strain was more than 64 mg/l and was higher than the peak penicillin serum level obtained in rats 30 min after the iv injection of 60 mg/kg, and in man after an oral dose of 2 g of phenoxymethyl penicillin. moreover timed kill curves performed in the presence of 64 mg/l of ...19853980332
does bacteremia occur during flexible sigmoidoscopy?up to 10% of patients may have bacteremia after rigid sigmoidoscopy. the aim of our study was to determine the frequency of bacteremia accompanying flexible sigmoidoscopy. blood samples for aerobic and anaerobic cultures were obtained before, during, and after flexible sigmoidoscopy in 100 patients who were examined a mean distance of 49.5 cm, range 15-60 cm, after a bowel preparation of two fleet enemas. in one patient, a transient bacteremia with streptococcus intermedius was documented and wa ...19854025278
fatty acid composition of streptococcus milleri.the cellular fatty acids of 31 strains belonging to the streptococcus milleri group were analysed by capillary gas-liquid chromatography. results were compared with findings from biochemical differentiation of the strains into streptococcus constellatus (two strains), streptococcus anginosus (16 strains) and streptococcus intermedius (13 strains). eight strains of various other streptococci were included as internal references, including three strains of streptococcus morbillorum, three strains ...19854043056
evidence for the generation of specific t suppressor lymphocytes by streptococcus intermedius.crude extracellular products of the streptococcus intermedius "cep-si" were able to strongly decrease the in vitro proliferation of stimulated human peripheral blood mononuclear cells (hpbmc) when evaluated by (3h)-thymidine and (3h)-leucine uptake. on the other hand, cep-si only slightly decreased this proliferation when hpbmc were either not stimulated or cultured in poor conditions or immature cells, i.e. thymocytes were used as target instead. also in vivo cep-si was ineffective when target ...19836191807
bacteroides fragilis, streptococcus intermedius and group b streptococci in ascending infection of pregnancy. an animal experimental study.by means of bacteriological, histological and immunofluorescence methods, the ascending spread of endocervically inoculated bacteria was studied in 14 pregnant sheep. 8 ewes near term were exposed to group b streptococci, bacteroides fragilis or streptococcus intermedius for 2-4 days. 6 other animals in mid pregnancy were exposed to one of the bacteria mentioned for 30 days. before inoculation, aerobic and anaerobic culture specimens from cervix and amniotic fluid were obtained. at delivery by c ...19836341180
comparison of three methods for anaerobe identification.in this study we evaluated the ability of three commercial methods, api 20a (analytab products, plainview, n.y.), minitek (bbl microbiology systems, cockeysville, md.), and anaerobe-tek (flow laboratories, inc., mclean, va.), to accurately identify 165 recent clinical and 38 stock isolates of anaerobic bacteria without supplemental tests or gas-liquid chromatography. strains included 89 bacteroides spp., 12 fusobacteria, 10 gram-positive, nonsporing rods, 43 clostridium spp., 15 streptococcus in ...19836355150
fractionation and characterization of the immunosuppressive substance in crude extracellular products released by streptococcus intermedius.the noncytotoxic immunosuppressive substance detected in crude extracellular products of streptococcus intermedius (cep-si) was fractionated by two steps of preparative isoelectric focusing in sucrose gradients using ampholytes of ph range from 3.5 to 6 and 4 to 5, respectively. the in vitro and in vivo suppressor effects of the most highly purified fraction of cep-si, designated fraction 3' (f3'ep-si), corresponded well with those of the original cep-si. f3'ep-si was sensitive to the effects o ...19816454698
nutritionally variant streptococci from corneal ulcers in horses.of 24 isolates of nutritionally variant streptococci recovered from equine corneal ulcers, 22 were tested for growth requirements, physiological and biochemical reactions, and susceptibility to different antimicrobial agents. satisfactory growth was obtained by supplementing blood agar and todd-hewitt broth with pyridoxal hydrochloride, and all of the media for the culture and the biochemical testing were supplemented with 0.002% of this substance. biochemical patterns of 12 of the isolates rese ...19846520222
[cariogenicity of streptococcus intermedius atcc 27335]. 19836581740
bacteriology of dental abscesses of endodontic origin.aspirates have been cultured from 10 dental abscesses of endodontic origin, all of which had penetrated beyond the bony alveolus to produce fluctuant swelling. sampling was by syringe aspiration. strict anaerobic techniques, including the use of an anaerobic chamber, were used for serial dilution and plating. randomly selected colonies (100) from each culture were purified, characterized, and identified. seventy percent of the bacterial isolates were either strict anaerobes or microaerophilic. o ...19836630460
antigenic relationships and rapid identification of peptostreptococcus species.antisera against whole cells of each peptostreptococcus species (p. anaerobius, p. micros, p. parvulus, and p. productus) were produced in rabbits. when these antisera were reacted against sonically disrupted cells and culture supernatant fluids in ouchterlony tests, lines of identity were obtained among the antigens from all the species and uninoculated culture medium. when the antisera were subsequently absorbed with the dehydrated culture medium used to grow the peptostreptococci, all cross-r ...19806769956
anaerobic bacteria in dentoalveolar infections.the bacteriology of 57 dentoalveolar infections was studied using optimal techniques to collect, transport and process specimens. there was an average of 4 bacterial species per specimen, and only 1/3 of the specimens held aerobes. among the aerobic bacteria, streptococci dominated and among the anaerobes the gram-negative rods, bacteroides ruminicola and fusobacterium nucleatum, were most frequently isolated followed by gram-positive cocci, in particular streptococcus intermedius. all aerobic i ...19816807917
trichosporon beigelii fungemia and cutaneous dissemination.trichosporon beigelii fungemia and multiple, purpuric, papular skin lesions developed on the chest wall and extremities of a 22-year-old man with acute granulocytic leukemia. histologically, the skin lesions demonstrated dermal budding yeasts, which were identified as t beigelii in culture. unexplained biventricular, congestive heart failure and sepsis wit streptococcus intermedius developed, and the patient died 28 days after his admission to the hospital.19826952820
experimental otitis media in gerbils and chinchillas with streptococcus pneumoniae, haemophilus influenzae, and other aerobic and anaerobic bacteria.to ascertain the usefulness of mongolian gerbils as an inbred model for otitis media, 52 mongolian gerbils (meriones unguiculatus, strain mont/tum) were compared with 26 chinchillas (chinchilla laniger) for susceptibility to streptococcus pneumoniae type 3. haemophilus influenzae type b, and a polymicrobic culture including anaerobes (streptococcus intermedius, propionibacterium acnes, staphylococcus epidermidis, and corynebacterium sp.). organisms were inoculated percutaneously into the superio ...19826979517
measurements of extracellular immunoglobulins as an index of b cell function.we have developed a method for measuring the activation or suppression of b cells in culture by determination of immunoglobulins released into the extracellular fluid, using a conventional, nonisotopic assay. to avoid the interference of human serum proteins in the assays, cultures were established in medium supplemented with fetal calf serum. this caused some b cell activation, as reflected by the release of small amounts of immunoglobulins (1.5 to 0.7 microgram/ml igg, 0.8 to 1.4 microgram/ml ...19807019022
natural history of aortic valve endocarditis in rats.sterile aortic vegetations were produced in rats by introducing a polyethylene catheter through the right carotid artery. the catheter was either left in place throughout the experiments or removed before bacterial challenge. bacterial endocarditis was uniformly produced by intravenous injection of 10(7) colony-forming units of staphylococcus aureus or streptococcus intermedius, whether the catheter was left in place or removed. however, in rats with the catheter left in place, bacterial multipl ...19827049946
effect of nitrogen mustard on natural history of right-sided streptococcal endocarditis in rabbits: role for cellular host defenses.cellular host defenses are considered to be ineffective in bacterial endocarditis; the microorganisms in infected vegetations are protected from phagocytic cells by dense layers of fibrin. to test this hypothesis, nitrogen mustard-induced agranulocytosis and leukopenia were produced in rabbits with right-sided streptococcal endocarditis. the spontaneous sterilization of tricuspid infection observed in the control animals was not present in the granulocytopenic, leukopenic animals. since the bact ...19827054124
successful prophylaxis against experimental streptococcal endocarditis with bacteriostatic antibiotics.because bacteriostatic concentrations of vancomycin are effective in prophylaxis against endocarditis due to streptococcus sanguis in rats, the efficacy of three other bacteriostatic antibiotics was investigated against three different streptococcal species that cause subacute endocarditis in humans: streptococcus intermedius. s. sanguis, and streptococcus mitior. rats were challenged by intravenous injection of 2 x 10(5) colony-forming units of streptococci 24 hr after intracardiac insertion of ...19827142750
production of deoxyribonuclease, ribonuclease, coagulase, and hemolysins by anaerobic gram-positive cocci.clinical isolates of peptococcus and peptostreptococcus species and streptococcus intermedius strains were obtained from local hospitals. after confirmed identification, each isolate was tested for the in vitro production of deoxyribonuclease, ribonuclease, coagulase, and hemolysins. of the 60 strains studied, 18 had enzymatic activity. the variability of enzyme production suggests that such assays are not suitable as an aid to identification of these organisms.19817229018
heterogeneity among 16s-23s rrna intergenic spacers of species within the 'streptococcus milleri group'.the 16s-23s rrna intergenic spacer has been suggested as a suitable region of the bacterial genome from which to derive useful taxonomic information, particularly with regard to identification at the species level. to investigate this approach as an aid to the identification of the three species comprising the 'streptococcus milleri group' (smg), the spacers of isolates of streptococcus intermedius, streptococcus anginosus and streptococcus constellatus were amplified by pcr and length polymorph ...19957545512
streptococcus anginosus, streptococcus constellatus and streptococcus intermedius. clinical relevance, hemolytic and serologic characteristics.a collection of 518 "streptococcus milleri" isolates recovered from clinical specimens was identified to the species level according to recently established criteria. streptococcus anginosus was the most frequently isolated species (59.3%), followed by s constellatus (30.3%) and s intermedius (10.4%). one third (34%) of all isolates were beta-hemolytic. the majority of s anginosus isolates were nonhemolytic, carried lancefield group f, or were nongroupable. most of the s constellatus isolates we ...19957572815
[antibacterial activities of cefmenoxime against recent fresh clinical isolates from patients in sinusitis].in order to evaluate antimicrobial activity of cefmenoxime (cmx), minimum inhibitory concentrations (mics) of cmx and control drugs were determined against clinical isolates from patients of sinusitis that were obtained in our laboratory from october of 1993 to march of 1994. the results are summarized as follows; 1. cmx showed strong antimicrobial activities against streptococcus pneumoniae, haemophilus influenzae and moraxella subgenus branhamella catarrhalis that were 3 major aerobic bacteria ...19957637194
bacteremia in conjunction with endodontic therapy.this study characterizes oral microorganisms believed to have spread from the root canal into the blood stream during and after endodontic therapy of teeth with asymptomatic apical periodontitis. microbiological samples were taken under aseptic conditions from the root canal of 26 single-rooted teeth in 26 patients. in the endodontic treatment of 13 of the patients (group 1), the first 3 reamers, sizes 15, 20 and 25, were used to a level 2 mm beyond the apical foramen. in the other 13 patients ( ...19957641631
detection of tet(m) and tet(o) using the polymerase chain reaction in bacteria isolated from patients with periodontal disease.the polymerase chain reaction was used to examine 114 tetracycline-resistant anaerobic and facultative anaerobic bacterial isolates from patients with periodontal disease for the tet(m) and tet(o) genes. a 740-base-pair fragment of the tet(m) gene was amplified from 84 of 114 isolates, and a 519-base-pair fragment of the tet(o) gene was amplified from 13 streptococcal isolates. six of 7 tetracycline-resistant isolates of veillonella spp. and tetracycline-resistant isolates of eubacterium spp. (n ...19957675524
peripheral lymphoid hyperplasia and central lymphoid depletion in mice treated with a bacterial b-cell mitogen (f3'ep-si/p90).in order to further understand the mechanism mediating the mitogenic and immunosuppressor effects of p90, a protein produced by streptococcus intermedius, flow cytometric studies were performed on peripheral and central lymphoid organs of mice treated with this protein. p90 induced a strong blastogenic b-cell response in the spleen and lymph nodes, followed by a slight but significant polyclonal t-cell activation. b-cell repertoire analysis indicated that polyclonal b-cell responses affected sim ...19937683441
degradative enzymes of oral streptococci.members of the streptococcus sanguis group (ssg) and streptococcus milleri group (smg) were screened for their ability to produce glycosidase, arylamidase (peptidase), protease, dextranase and glycosyltransferase activities. species within each group produced unique patterns of activity. the most commonly produced glycosidases were beta-d-glucosidase, beta-d-galactosidase, n-acetyl-beta-d-glucosaminidase and n-acetyl-beta-d-galactosaminidase and the least commonly produced glycosidase activity w ...19957786231
streptococcus intermedius spondylodiskitis. 19947843188
microbiota of rapidly progressive periodontitis lesions in association with clinical parameters.the microbial population in 73 rapidly progressive periodontitis (rpp) lesions in 10 young adults aged 25 to 35 years (5 males, 5 females) was studied in relation to the clinical parameters probing depth, bleeding on probing, and suppuration, which were recorded at the sampled sites. porphyromonas gingivalis was found to predominate (26.7%) in 73 periodontal lesions with clinical probing depth > 6 mm, followed by bacteroides forsythus (23.6%), and prevotella intermedia (15.7%). the prevalence of ...19947853132
degradation of immunoglobulin g by periodontal bacteria.several subgingival microorganisms were tested for their ability to utilize human immunoglobulin g (igg) as a substrate for growth. this was done using a protein-free chemically defined medium, supplemented with igg. stimulation of growth was observed for capnocytophaga ochracea, porphyromonas asaccharolytica, porphyromonas endodontalis, porphyromonas gingivalis, prevotella intermedia, prevotella oralis, lactobacillus catenaforme and streptococcus intermedius. immunoelectrophoresis, sodium dodec ...19947870469
two cases of lateral sinus thrombosis presenting with extracranial head and neck abscesses.two cases of lateral sinus thrombosis in fit adults with no previous otological history are presented. one case occurred in association with bezold's abscess and followed mastoiditis which was masked by previous antibiotic treatment. the other case occurred in association with an occipitoparietal scalp abscess and there was no obvious preceding middle ear infection. the causative bacteria were streptococcus milleri and a variant streptococcus intermedius. lateral sinus thrombosis is discussed.19947964144
degradation of hyaluronate by streptococcus intermedius strain uns 35.streptococcus intermedius strain uns 35, a brain abscess isolate, produced extracellular hyaluronidase when grown in brain heart infusion broth. chemical assays with this enzyme indicated that hyaluronate depolymerisation resulted in the formation of carbohydrate moieties with n-acetylglucosamine at the reducing terminal and containing an unsaturated carbon-carbon double bond. the nature of the products of this hyaluronidase were investigated further by high-field (400 mhz) proton (1h) nmr spect ...19947966219
microaerophilic streptococci as a significant pathogen: a twelve-year review.one hundred twenty three microaerophilic streptococci (ms) were isolated from 4,603 specimens submitted for the identification of anaerobic bacteria over the course of 12 years. the ms included 16 streptococcus constellatus, 43 streptococcus intermedius and 21 gemella morbillorum. a total of 81 ms isolates (66%) identified from 75 patients were considered to cause infection. predisposing or underlying conditions were noted in 48 (64%) patients. the most common conditions were previous surgery (1 ...19947996059
production of specific glycosidase activities by streptococcus intermedius strain uns35 grown in the presence of mucin.an isolate of streptococcus intermedius from a brain abscess showed neuraminidase (sialidase), beta-d-galactosidase, n-acetyl-beta-d-glucosaminidase and n-acetyl-beta-d-galactosaminidase activities. the optimal ph values of these enzymes were 5.5-6.0, 5.5-6.0, 5.0-5.5 and 5.0-5.5, respectively. the km of the enzymes varied according to whether the type of substrate was chromogenic or fluorogenic; sialidase was most active at the lowest substrate concentrations, with a km of 0.01 mm. in semi-defi ...19948064838
microaerophilic streptococcal infection in children.a total of 148 isolates of microaerophilic streptococci (ms) (47 streptococcus constellatus, 43 streptococcus intermedius and five gemella morbillorum) were cultured from 123 children. there were predisposing conditions in 47 (38%) patients of which most common were previous surgery (14), trauma (11), malignancy (9) diabetes (6) and immunodeficiency (5). ms were the only bacteria isolated from 12 (10%) patients and mixed infections were encountered in 111, when the number of isolates varied betw ...19948089512
[evaluation of phenotype characteristics of streptococci in the identification of the streptococcus anginosus (streptococcus milleri) group].microorganisms referred to as streptococcus anginosus (streptococcus milleri) group which consist of s. anginosus, s. intermedius and s. constellatus is very difficult to identify to the species level, because of their diversity of biochemical, hemolytic and serological characteristics and because of confusion surrounding their taxonomy. identification procedures on three isolates from primary sterile sites of three different patients, using api 20a system are described. streptococcus intermediu ...19938170272
evaluation of a one-step surgical preparation technique in dogs.the efficacy of a 1-step surgical preparation technique for skin of dogs prior to elective ovariohysterectomy was evaluated. dogs randomly assigned to group 1 (n = 30) had their skin prepared for surgery by use of a 2-step method, whereas the skin of dogs in group 2 (n = 30) was prepared for surgery by use of a commercially available product for a 1-step technique. culture plates for quantitative bacterial counts were applied to the proposed incision site on dogs under general anesthesia after h ...19938226216
septic pulmonary embolism due to periodontal disease.three weeks following a toothache, a 56-year-old man developed cough, sputum, fever, and pleuritic chest pain. he had mild periodontal disease and his chest radiographs and chest computed tomographic (ct) scans showed multiple pulmonary nodules. the ct scan strongly suggested septic pulmonary embolism. aspirated pus from one of the nodules yielded pure growth of streptococcus intermedius. lesions resolved with antimicrobial therapy. the usual predisposing factors for septic pulmonary embolism we ...19938252994
albumin-binding proteins on the surface of the streptococcus milleri group and characterization of the albumin receptor of streptococcus intermedius c5.members of the streptococcus milleri group (smg) that react with lancefield group c antisera were shown to bind large amounts of albumin although there was no direct relation between these two properties as polyclonal antisera to lancefield group c antigen did not prevent the binding of albumin. there was a specificity for albumin binding, with albumin from man, monkeys, cat, dog and mouse being bound to a greater degree than albumin from cow, horse, goat or rabbit. gold-labelled albumin was sho ...19938254315
haemagglutinating activities of oral strains of streptococcus milleri group.of a total 148 strains of streptococcus milleri group, 66 agglutinated sheep erythrocytes. the haemagglutinating strains were confined to serotypes g, h, i, j or were untypeable, and had no lancefield group antigens (a to g and k), all of which have been shown to belong to streptococcus intermedius. cell surface hydrophobicity did not significantly differ even between the agglutinating and non-agglutinating s. intermedius strains. the haemagglutinating activity of strain 0813-1 (serotype i) was ...19938255256
bactericidal effects of povidone-iodine solution to oral pathogenic bacteria in vitro.seven species of periodontal pathogenic bacteria (bacteroides, gingivalis, bacteroides intermedius, bacteroides melaninogenicus, fusobacterium nucleatum, actinobacillus actinomycetemcomitans, capnocytophaga spp., eikenella corrodens) and two control species (streptococcus intermedius, pseudomonas aeruginosa) were selected in order to study the bactericidal effects of 10% povidone-iodine (pvp-i) aqueous solution in vitro. the pvp-i solution was diluted to 10, 20, 50, 100, 200, 400, 800, 1600, 320 ...19938290445
enzymatic differentiation and biochemical and serological characteristics of the clinical isolates of streptococcus angiosus, s. intermedius and s. constellatus.of the 29 'streptococcus milleri' strains tested, all thirteen streptococcus intermedius (dna homology group 2) strains but none of the thirteen streptococcus anginosus (group 1) strains produced beta-n-acetylglucosaminidase, beta-n-acetylgalactosaminidase, alpha-n-acetylneuraminidase, beta-galactosidase, alpha-glucosidase, and hyaluronidase. the three streptococcus constellatus (group 3) strains produced only the latter two. glycosidase production divided 274 clinical isolates into 103 s. angin ...19938295553
chondroitin sulfate depolymerase and hyaluronidase activities of viridans streptococci determined by a sensitive spectrophotometric assay.sensitive spectrophotometric assays for the detection of bacterial chondroitin sulfate depolymerase and hyaluronidase activities were developed by using stains-all (1-ethyl-2-[3-(1-ethylnaphtho-[1,2-d]thiazolin-2-ylidene)-2- methylpropenyl]naphtho-[1,2-d]thiazolium bromide). stains-all interacts with hyaluronic acid to produce a shift in the absorption spectrum with a distinct absorption peak between 620 and 660 nm, while chondroitin sulfate interacts to form a distinct shoulder between 440 and ...19938315011
recent taxonomic changes for anaerobic gram-positive and selected gram-negative organisms.the strains formerly classified as streptococcus anginosus or "streptococcus milleri" have now been recognized as three distinct species, streptococcus anginosus, streptococcus constellatus, and streptococcus intermedius. streptococcus morbillorum has been transferred into the genus gemella. four new species within the genus peptostreptococcus were recently named. a recent addition to the genus clostridium is clostridium argentinense, which includes clostridium botulinum type g. two new species ...19938324113
the isolation and characterization of milleri group streptococci from dental periapical abscesses.reports of the isolation of streptococci from dental abscesses have shown an association of the "s. milleri" group with such lesions. there has been considerable confusion regarding the taxonomy of these organisms, but the milleri group has recently been reclassified into three distinct species: streptococcus anginosus, streptococcus constellatus, and streptococcus intermedius. in this study, 45 samples from dental periapical abscesses were examined. milleri group streptococci were isolated from ...19938360361
hydrolytic enzymes of streptococcus anginosus, streptococcus constellatus and streptococcus intermedius in relation to infection.a collection of 518 'streptococcus milleri' strains was tested for the presence of hydrolytic enzymes, and the results were related to the clinical significance of the strains. ribonuclease activity was equally distributed among the three species while hyaluronidase activity was linked to streptococcus intermedius and streptococcus constellatus. both enzymes were not significantly associated with infection-related strains. deoxyribonuclease and chondroitin sulfatase activity tended to be present ...19958536735
viridans streptococci isolated from the bloodstream. relevance of species identification.over a 4-year-period, 104 isolates belonging to the viridans streptococci were recovered from the blood and identified to the species according to beighton and co-workers. streptococcus oralis was the species most frequently recovered from patients in the hematology unit [29 of 39 (74%)]. streptococcus mitis ranked second [seven of 39 (18%)]. both species were associated with oromucositis. isolates presently identified as s. oralis are mainly those previously identified as s. mitis or streptococ ...19958565415
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