| influence of the culture medium on the synthesis of alpha-d-glucans by streptococcus cricetus aht. | three different alpha-d-glucosyltransferases (gtfs) were separated from culture filtrates of streptococcus cricetus strain aht grown in a complex, standard medium in batch culture or under defined conditions of growth in the chemostat. two of the enzymes (gtf-s1 and gtf-s2) converted sucrose into branched, soluble dextrans, and the third (gtf-i) produced a relatively linear, water-insoluble, predominantly (1----3)-linked alpha-d-glucan. when the organism was grown in complex medium modified by t ... | 1992 | 1379884 |
| reduced nicotinamide adenine dinucleotide oxidase involvement in defense against oxygen toxicity of streptococcus mutans. | the growth inhibition of the streptococcus mutans group, including streptococcus mutans, streptococcus cricetus, streptococcus rattus and streptococcus sobrinus, on glucose by oxygen and the properties of reduced nicotinamide adenine dinucleotide (nadh) oxidase induced by oxygen, using a representative oxygen-tolerant strain, were examined. the growth response to oxygen varied among strains and correlated with the level of nadh oxidase activity in the cell extract. the induced synthesis of nadh ... | 1992 | 1494456 |
| chelating agents inhibit activity and prevent expression of streptococcal glucan-binding lectins. | several of the cariogenic mutans streptococci produce cell wall-associated glucan-binding lectins (gbls). the lectins bind alpha-1,6-linked glucans and have no affinity for other polysaccharides or anomeric linkages. when citrate or lactate was included in the growth medium, expression of the activities of the gbls of streptococcus cricetus and s. sobrinus was prevented. furthermore, chelating agents, including citrate, lactate, edta, and acetylacetone, were able to reversibly inhibit glucan-ind ... | 1992 | 1500189 |
| adhesion of mutants streptococci to glass with and without a salivary coating as studied in a parallel-plate flow chamber. | deposition and adhesion to glass with and without a salivary coating in a parallel-plate flow chamber were studied with four strains of mutans streptococci. stationary-state adhesion of the strains to uncoated glass ranged from 0.3 x 10(6) cm-2 (streptococcus rattus hg218) to 12.7 x 10(6) cm-2 (streptococcus sobrinus hg1025) and generally decreased after saliva coating of the glass. the poor adhesion found for s. rattus hg218 to both uncoated and saliva-coated glass could be due to its relativel ... | 1992 | 1573082 |
| immunological relationships between glucosyltransferases synthesizing insoluble glucan from streptococcus cricetus, streptococcus sobrinus and streptococcus downei. | the mr values and isoelectric points of glucosyltransferases synthesizing insoluble glucan (gtf-is) were determined, and the immunological relationships between them studied. the gtf-i enzymes were from streptococcus cricetus (mutans group serotype a), streptococcus sobrinus (mutans group serotypes d and g) and streptococcus downei (mutans group serotype h). by double immunodiffusion tests, the gtf-i enzymes from the three species possessed a common antigenic determinant; in addition, the gtf-i ... | 1991 | 1720167 |
| comparison of the physicochemical surface properties of streptococcus rattus with those of other mutans streptococcal species. | mutans streptococci comprise a group of seven closely related, yet distinct species. the distinction between the four species used in this study, namely streptococcus sobrinus, streptococcus cricetus, streptococcus rattus, and streptococcus mutans, has been made only recently on the basis of dna homologies. in order to determine if there is a difference in the physicochemical surface properties of these species, strains were characterized by contact angles, zeta potentials and isoelectric points ... | 1991 | 1810653 |
| effects of propolis on dental caries in rats. | propolis, the resinous hive product collected by bees, is important in the defense of the hive. the effects of propolis on growth and glucosyltransferase activity of streptococcus sobrinus 6715, streptococcus mutans ps14 and streptococcus cricetus omz61 in vitro, and on dental caries in rats infected with s. sobrinus 6715 were investigated. propolis had antimicrobial activity against s. sobrinus, s. mutans and s. cricetus, and inhibited both water-insoluble glucan synthesis and glucosyltransfera ... | 1991 | 1836157 |
| streptococcus cricetus and streptococcus rattus bind to different segments of collagen molecules. | strains of streptococcus cricetus and streptococcus rattus exhibited striking differences in their ability to bind to different types of collagen. for example, s. cricetus aht bound in highest numbers to hydroxyapatite (ha) treated with human type v collagen, while rat type i collagen was ineffective. in contrast, human type v collagen was least effective in promoting attachment of s. rattus lb-1, while treatment with rat or human type i collagen was effective. adsorption of both species to huma ... | 1990 | 1964204 |
| binding of streptococci of the "mutans" group to type 1 collagen associated with apatitic surfaces. | the present study surveyed the ability of 21 strains of cariogenic streptococci of the "mutans" group to bind to human type 1 collagen adsorbed on hydroxyapatite (ha) surfaces. all strains of streptococcus cricetus (serotype a) and streptococcus rattus (serotype b) tested attached strongly to collagen-treated ha (c-ha). streptococcus mutans strains of serotype c and streptococcus sobrinus strains of serotype d or g attached poorly; or not at all, to c-ha. s. mutans strains omz-175 (serotype f) a ... | 1990 | 2080067 |
| enzyme immunoassay and monoclonal antibodies for immunological studies of the mutans group of streptococci. | monoclonal antibodies (mabs) were prepared from myeloma cells and splenocytes of mice immunized with streptococcus cricetus hs1 or streptococcus downei mf25 cells. various makes of immunoplates were coated with whole cells or purified polysaccharide antigens of oral streptococcal strains and tested for reactivity in enzyme immunoassay (eia) using the mabs. it was revealed that different immunoplates had different reactivities. it was also shown that the eia system and mabs were useful for the im ... | 1990 | 2099287 |
| cross-reactive polysaccharide antigens (types a, d, and h) of the mutans group of streptococci: different molecular forms of the type as distinguishable by monoclonal antibodies. | as compared to the previous precipitin inhibition tests differences were found in the reactivities of monoclonal antibodies (mabs), a-4 and a-84 with streptococcus cricetus (serotype a) in an enzyme immunoassay using whole cells, purified cell wall antigen and haptenic sugars coated onto microtitre wells. investigation into the differences led to the finding that the purified antigen from s. cricetus cells consisted mainly of three forms with different molecular weights and sugar contents. mab a ... | 1990 | 2184819 |
| molecular cloning and characterization of the spab gene of streptococcus sobrinus. | a gene of streptococcus sobrinus 6715 (serotype g) designated spab and encoding a surface protein antigen was isolated from a cosmid gene bank. a 5.4 kb hindiii/avai dna fragment containing the gene was inserted into plasmid pbr322 to yield plasmid pxi404. analysis of plasmid-encoded gene products showed that the 5.4 kb fragment of pxi404 encoded a 195 kda protein. southern blot experiments revealed that the 5.4 kb chromosomal insert dna had sequence similarity with genomic dna of s. sobrinus 67 ... | 1990 | 2230719 |
| cariological assessment of leucrose [d-glucopyranosyl-alpha(1----5)-d-fructopyranose] as a sugar substitute. | leucrose [d-glucosyl-alpha(1----5)-d-fructopyranose] prepared by microbial-enzymatic transglycosidation from sucrose, is the first alpha(1----5)-linked disaccharide which possesses excellent nutritional properties with regard to metabolic utilization and is well tolerated. the aim of the present work was to assess its cariogenic potential. yeast invertase was shown to be inhibited by leucrose in a noncompetitive way, while hydrogenated leucrose (leucritol) acted as an activator. plaque polysacch ... | 1989 | 2504493 |
| purification of a fourth glucosyltransferase from streptococcus sobrinus. | recently, we found a novel primer-independent, water-soluble glucan synthase as a fourth glucosyltransferase (gtf) in a culture supernatant of strain aht-k of streptococcus sobrinus (y. yamashita, n. hanada, and t. takehara, biochem. biophys. res. commun. 150:687-693, 1988). in the present study, four kinds of purified gtfs, including the novel gtf, were prepared. they were composed of two primer-dependent gtfs and two primer-independent gtfs. of the primer-dependent gtfs, one was a water-insolu ... | 1989 | 2530209 |
| molecular and immunochemical characterization of recombinant escherichia coli containing the spaa gene region of streptococcus sobrinus. | we identified and characterized a recombinant escherichia coli containing the entire gene for surface protein antigen a (spaa) of streptococcus sobrinus 6715. the recombinant e. coli was isolated from a cosmid gene bank of size-fractionated s. sobrinus dna fragments, and recombinants expressing the spaa protein were detected immunologically. subcloning experiments showed that the dna sequences encoding the spaa protein could be isolated on two contiguous ecori fragments, 3.7 and 3.3 kilobases (k ... | 1989 | 2543627 |
| comparative cariogenicity and dental plaque-forming ability in gnotobiotic rats of four species of mutans streptococci. | the four species, each represented by two strains, all produced high levels of fissure caries in the upper and lower jaws of gnotobiotic fisher rats. streptococcus rattus strains and streptococcus mutans nctc 10832 gave low levels of approximal caries, whereas streptococcus cricetus, streptococcus sobrinus and strep. mutans nctc 10449 gave high levels. the only strains to produce buccal surface lesions were strep. sobrinus and strep. cricetus. all strains were able to form dense plaque in the ce ... | 1989 | 2610617 |
| characterisation of monoclonal antibodies to common protein epitopes on the cell surface of streptococcus mutans and streptococcus sobrinus. | three monoclonal antibodies (mab) were prepared against a cell surface antigen which cross-react between streptococcus mutans (serotypes c, e and f) and streptococcus sobrinus (serotypes d and g). two of the mab also recognise a determinant on the surface of streptococcus cricetus (serotype a). the common antigen shared between s. mutans and s. sobrinus was demonstrated by western blotting to be about 200 kd in size. this antigen is shared not only by the cell surfaces of serotypes a, c, d, e, f ... | 1989 | 2639299 |
| molecular characterization of a surface protein antigen gene from serotype c streptococcus mutans, implicated in dental caries. | the complete nucleotide sequence of the gene for a cell-surface protein antigen (pac) of streptococcus mutans mt8148 (serotype c) was determined. the pac gene consisted of 4695 bp and coded for a 170773d protein. the pac gene product contained a putative 38 amino acid signal peptide, resulting in a 166817d mature protein. a potential promoter sequence and a putative shine-dalgarno sequence preceded the open reading frame. two internal repeating amino acid sequences were present in the pac. one r ... | 1989 | 2761390 |
| comparison of extracellular protein profiles of seven serotypes of mutans streptococci grown under controlled conditions. | extracellular proteins produced by the four human commensal species of mutans streptococci were analysed. the organisms used were streptococcus mutans, serotypes c, e and f, streptococcus cricetus, serotype a, streptococcus rattus, serotype b, and streptococcus sobrinus, serotypes d and g. they were grown in continuous culture at different generation times and ph values in media containing either glucose or fructose to determine the extent of variation in extracellular protein production that co ... | 1986 | 2945902 |
| molecular cloning and characterization of scrb, the structural gene for the streptococcus mutans phosphoenolpyruvate-dependent sucrose phosphotransferase system sucrose-6-phosphate hydrolase. | a dna fragment encoding the sucrose-6-phosphate hydrolase component of the streptococcus mutans phosphoenolpyruvate-dependent sucrose phosphotransferase system has been recovered from a plasmid-based genomic library of strain gs5. the locus, designated scrb, was found to reside within a 2.9-kilobase-pair restriction fragment present on the chimeric molecule pva1343 (7.3 kilobase pairs). minicell analysis of pva1343-directed translation products revealed that the scrb product synthesized in esche ... | 1986 | 3009399 |
| in-vitro adherence of oral streptococci in the presence of sucrose and its relationship to cariogenicity in the rat. | streptococcus sanguis i gave a significantly greater percentage coverage (cell-pellicle attachment) of saliva-coated glass in the presence of sucrose than did strep. sanguis ii (p less than 0.025), and both these gave greater percentage coverages than the other species tested, between which no significant differences were noted. there was a large number of significant differences in clump size (cell-cell attachment) between species-pairs. among the mutans streptococci, there were significant dif ... | 1988 | 3166609 |
| glucan-binding factor in saliva. | high-molecular-weight polymers of alpha-1,6-linked d-glucans are insoluble in alcohol solutions. whole, but not parotid, saliva prevented the precipitation of d-glucans by 80% (vol/vol) ethanol, showing that the whole saliva contained a factor which complexed with the glucan to render it alcohol soluble. the glucan-binding factor was retained on a column of sephacryl s-200 which had been preequilibrated with 80% ethanol. the factor was then eluted with water. passive hemagglutination assays reve ... | 1988 | 3169992 |
| specificity of the glucan-binding lectin of streptococcus cricetus. | the specificity of the glucan-binding lectin (gbl) of streptococcus cricetus aht was determined. examination of the kinetics of aggregation of cell suspensions with glucans containing various percentages of alpha-1,6, alpha-1,4, alpha-1,3, and alpha-1,2 anomeric linkages revealed that only glucans with at least 80% alpha-1,6 linkages promoted strong aggregation. moreover, only linear glucans with molecular weights greater than 5 x 10(5) were capable of causing rapid aggregation of the bacteria. ... | 1988 | 3397177 |
| expression of the glucan-binding lectin of streptococcus cricetus requires manganous ion. | streptococcus cricetus aht exhibited a requirement for manganese for growth and expression of the glucan-binding lectin. while low concentrations of manganese (0.1 to 10 microm) were able to support growth, higher concentrations (greater than 100 microm) were required for full expression of the glucan-binding lectin. the manganous-aquo ion may be important in cellular adhesion and accumulation processes in dental plaque. | 1988 | 3397191 |
| characterization of a rat salivary sialoglycoprotein complex which agglutinates streptococcus mutans. | rat saliva agglutinated streptococcus mutans ingbritt and nctc 10449 and streptococcus sanguis nctc 7864 but not s. mutans nctc 10921, gs 5, or lm 7, streptococcus sobrinus 6715-13 or omz 65, or streptococcus cricetus hs 6, as measured turbidometrically. the specificity of agglutination by rat saliva was the same as that by human saliva. agglutination was associated with a mucin complex (rat salivary agglutinin complex [rs-a]) of sulfated sialoglycoproteins, with a trace of associated lipid and ... | 1987 | 3570462 |
| penicillin-induced lysis of streptococcus mutans. | treatment of streptococcus mutans gs-5 cells with concentrations of penicillin g within a relatively narrow range resulted in substantial lysis. this penicillin-induced lysis was dependent upon cell density and ph of the lysis medium. other oral streptococci (streptococcus sobrinus, streptococcus rattus, and streptococcus cricetus) also demonstrated substantial levels of penicillin-induced lysis under appropriate conditions. lesser degrees of lysis were seen in a related organism, streptococcus ... | 1984 | 6500698 |
| prevalence, distribution of serotypes, and cariogenic potential in hamsters of mutans streptococci from elderly individuals. | the prevalence of mutans streptococci (streptococcus mutans, streptococcus cricetus, streptococcus sobrinus, and streptococcus rattus) was determined in the salivas of 169 elderly individuals ranging in age from 60 to 87 years. approximately 40% of these individuals were edentulous and wore full upper and lower dentures. with the exception of a higher proportion of saliva counts below 1,000 cfu/ml in the full-denture wearers, the prevalence and the serotype and species distributions of the mutan ... | 1983 | 6874072 |
| comparison of aminopeptidase activities in four strains of mutans group oral streptococci. | in this study, native cells of streptococcus mutans va-29r and streptococcus rattus fa-1 displayed significantly higher aminopeptidase activity than did cells of streptococcus cricetus aht or streptococcus sobrinus 6715 toward the nitroanilide derivatives of leucine, alanine, methionine, arginine, and lysine. these differences in cellular aminopeptidase activity led us to investigate the subcellular localization of the aminopeptidase in these mutans group streptococci. following conversion of na ... | 1993 | 8418040 |
| flow chamber study of the adhesion of prevotella intermedia to glass after preconditioning with mutans streptococcal species: kinetics and spatial arrangement. | the adhesion kinetics and spatial arrangement of a clinical isolate of prevotella intermedia on bare glass and on glass which had been previously exposed to streptococcus rattus or streptococcus cricetus were studied using a parallel plate flow system and image analysis. when s. cricetus was the preconditioning organism the initial deposition rate and efficiency of p. intermedia were reduced even though very few s. cricetus adhered during flow with p. intermedia. preconditioning with s. rattus h ... | 1993 | 8459781 |
| effect of porphyromonas gingivalis atcc 33277 vesicle on adherence of streptococcus mutans omz 70 to the experimental pellicle. | the vesicles of porphyromonas gingivalis atcc 33277 strongly aggregated streptococcus cricetus, s. rattus, and s. mutans, but poorly aggregated s. sobrinus. the adherence of s. mutans omz 70 to hydroxyapatite (ha) coated with whole saliva was increased in parallel with the quantity of the vesicles. the significant increase of adherence of s. mutans omz 70 by the vesicles was also observed on the ha coated with parotid saliva, submandibular saliva, serum, and type i collagen. these findings sugge ... | 1995 | 8569538 |
| inhibitory effects of funoran on the adherence and colonization of oral bacteria. | funoran, a sulfated polysaccharide extracted from the seaweed gloiopeltis furcata, strongly inhibited the adsorption of mutans streptococci to saliva-coated hydroxyapatite (s-ha) used as an experimental pellicle and strongly desorbed cariogenic mutans streptococci pre-adsorbed to s-ha. colonization inhibition and anticariogenic effects of funoran were also investigated in experimental rats. the colonization of streptococcus cricetus e49 inoculated on the molar teeth of experimental rats administ ... | 1996 | 9151580 |
| identification of clinically relevant viridans streptococci by analysis of transfer dna intergenic spacer length polymorphism. | the utility of pcr analysis of transfer dna intergenic spacer length polymorphism (tdna-ilp) for the identification to the species level of clinically relevant viridans streptococci was evaluated with a collection of reference strains of 15 species of the salivarius, anginosus, mitis and mutans rrna homology groups. pcr products generated by using fluorescent, outwardly directed, consensus tdna primers were analysed by electrophoresis on denaturating polyacrylamide gels and by laser fluorescence ... | 1999 | 10555340 |
| cloning and dna sequencing of the surface protein antigen i/ii (paa) of streptococcus cricetus. | we have cloned and sequenced the gene encoding the surface protein antigen paa (antigen i/ii family) from streptococcus cricetus e49 (serotype a) using degenerate pcr. the deduced amino acid sequence of paa reveals two repeating regions (a region; alanine-rich region, p region; proline-rich region). two additional tandem repeats were found in the a region and part of the p region was deleted compared to antigen i/ii. homology and phylogenetic analyses reveal that paa is homologous to streptococc ... | 2001 | 11267788 |
| recommended conservation of the names streptococcus sanguis, streptococcus rattus, streptococcus cricetus, and seven other names included in the approved lists of bacterial names. request for an opinion. | with reference to the first principle of the international code of nomenclature of bacteria, which emphasizes stability of names, it is proposed that the original names streptococcus sanguis, streptococcus rattus, streptococcus cricetus, erwinia ananas, eubacterium tarantellus, lactobacillus sake, nitrosococcus oceanus, pseudomonas betle, rickettsia canada and streptomyces rangoon, all included in the approved lists of bacterial names, be conserved. request for an opinion. | 2001 | 11321121 |
| genetic studies on reference strains of mutans streptococci. | twenty four reference strains (serotype a-h) belonging to the mutans group of streptococci were compared for dna fragment patterns of rdna after treatment with hind iii. it was shown that streptococcus cricetus (serotype a), s. rattus (serotype b), and s. downei (serotype h) reveals comparatively homogeneous patterns while s. mutans (serotype c, e and f) exhibits differences between the different serotypes as well as within single serotypes. s. sobrinus had an intermediary diversity. these data ... | 2002 | 12501994 |
| classification of oral streptococci by two-dimensional gel electrophoresis with direct activity stain for glycosyltransferases. | thirty eight strains of oral streptococci were divided into six types by two-dimensional gel electrophoresis (2-de) followed by glycosyltransferase (glt) activity stain: type 1, streptococcus mutans; type 2, streptococcus rattus; type 3, streptococcus sobrinus and streptococcus downei; type 4, streptococcus cricetus; type 5, streptococcus salivarius; and type 6, streptococcus sanguis, streptococcus oralis and streptococcus gordonii. in types 1, 2 and 5, two glucosyltransferases synthesizing wate ... | 2003 | 12753469 |
| identification of another surface protein antigen i/ii gene, paab, and a putative transcriptional regulator gene, par, from streptococcus cricetus. | the flanking region of the antigen i/ii gene, paaa, in streptococcus cricetus was examined using the gene-walking technique. in the region downstream of the paaa gene, another antigen i/ii gene designated as paab was found. the paab gene was disrupted at the alanine-rich region (a region) by a novel insertion sequence element, isscr1. isscr1 is a member of the is982 family and is composed of a 962-bp sequence and duplicated target dna (the sequence 5'-tagctaaat-3') resulting from its insertion. ... | 2004 | 15329493 |
| nucleotide sequencing and transcriptional analysis of two tandem genes encoding glucosyltransferase (water-soluble-glucan synthetase) in streptococcus cricetus hs-6. | two tandem genes encoding glucosyltransferase synthesizing water-soluble glucan (gtf-s) were cloned from the lambda gene library of streptococcus cricetus hs-6 (serotype a) using anti-gtf-s antibody, and the nucleotide sequences were analyzed. the two genes (orf1 and orf2) were identified as streptococcal glucosyltransferases based on the following evidence: [1] the deduced amino acid sequences of their products have an active site for catalytic action and c-terminal repeated units for dextran b ... | 2000 | 11092239 |
| identification and characterization of a dextranase gene of streptococcus criceti. | the dextranase gene, dex, was identified in streptococcus criceti strain e49 by degenerate pcr and sequenced completely by the gene-walking method. a sequence of 3,960 nucleotides was determined. the dex gene encodes a 1,200-amino acid protein, which has a calculated molecular mass of 128,129.91 and pi of 4.15 and is predicted to be a cell-surface protein. the deduced amino acid sequence of dex showed homology to s. downei dextranase (63.9% identity). phylogenetic analysis revealed the similarit ... | 2007 | 17704634 |
| sequence and phylogenetic analyses of novel glucosyltransferase genes of mutans streptococci isolated from pig oral cavity. | nucleotide sequences of water-insoluble glucan-producing glucosyltransferase (gtf) genes of new mutans streptococci isolated from pig oral cavity, streptococcus orisuis jcm14035, and of streptococcus criceti hs-6 were determined. the gtf gene of s. orisuis jcm14035 consisted of a 4,401 bp orf encoding for a 1,466 amino acids, and was revealed to belong to the gtfi group. the percent homology of amino acid sequence of the gtf-i from s. orisuis and s. criceti are 95.0%, however, this score ranges ... | 2008 | 18545970 |
| the macrophage pattern recognition scavenger receptors sr-a and cd36 protect against microbial induced pregnancy loss. | microbial products can act via stress-induced signaling cascades to link dysregulated endogenous microbiota to immune activation (e.g., macrophages) and pregnancy loss. our previous studies demonstrated that mice deficient in the macrophage pattern recognition scavenger receptors, sr-a and cd36, are more susceptible to inflammatory complications including gut leakiness and experimental colitis. we hypothesized that bacterial penetration of the maternal mucosal surfaces and replication in embryon ... | 2011 | 20711846 |
| identification of a2059g 23s rrna and g439a rplc gene mutations in streptococcus criceti strain omz 61, a strain resistant to azithromycin, josamycin and clindamycin. | streptococcus criceti is a cariogenic organism that belongs to the mutans streptococci. of the four s. criceti strains, strain omz 61 has been identified as being resistant to erythromycin. antimicrobial susceptibility testing showed that strain omz 61 is also resistant to azithromycin, josamycin and clindamycin but susceptible to tetracycline and tiamulin. dna hybridization analysis of the 23s rrna genes revealed that the hybridization patterns in strain omz 61 differed from those in the other ... | 2015 | 26725523 |
| gbpc gene repertoire variation among mutans streptococci. | the human dental caries pathogen streptococcus mutans harbors one glucan-binding wall-anchored protein gene, gbpc, and another human pathogen, streptococcus sobrinus, has 4 gbpc homologues. as no 4 gbpc homologues have so far been detected in other mutans streptococci, however, we cannot say whether those in s. sobrinus are paralogous or orthologous. therefore, the purpose of this study was to identify and sequence the gbpc/dbl genes in streptococcus downei and streptococcus criceti. the finding ... | 2012 | 22790333 |
| molecular characterization of the dextran-binding lectin b gene dblb of streptococcus criceti in streptococcus mutans strain gs-5 with mutations in both gbpc and spap genes. | streptococcus mutans, a cariogenic agent, has a glucan-binding protein gene, gbpc, and s. criceti possesses four gbpc homologs, including dbla and dblb, as does s. sobrinus. the s. criceti dblb gene encodes a 1,717-amino-acid protein having two repetitive alanine-rich and proline-rich regions and an lpxtg motif, which is recognized by the sortase srta, near the c terminus. reverse transcription-pcr analysis indicated no cotranscription of the dbla and dblb genes of s. criceti. as we could not ob ... | 2014 | 25224970 |
| streptococcus oriloxodontae sp. nov., isolated from the oral cavities of elephants. | two strains were isolated from oral cavity samples of healthy elephants. the isolates were gram-positive, catalase-negative, coccus-shaped organisms that were tentatively identified as a streptococcal species based on the results of biochemical tests. comparative 16s rrna gene sequence analysis suggested classification of these organisms in the genus streptococcus with streptococcus criceti atcc 19642(t) and streptococcus orisuis num 1001(t) as their closest phylogenetic neighbours with 98.2 and ... | 2014 | 25139416 |
| identification and characterization of an autolysin gene, atla, from streptococcus criceti. | atla of streptococcus mutans is a major autolysin and belongs to glycoside hydrolase family 25 with cellosyl of streptomyces coelicolor. the autolysin gene (atla) encoding atla was identified from s. criceti. atla of s. criceti comprises the signal sequence in the n-terminus, the putative cell-wall-binding domain in the middle, and the catalytic domain in the c-terminus. homology modeling analysis of the catalytic domain of atla showed the resemblance of the spatial arrangement of five amino aci ... | 2012 | 23124745 |
| streptococcus orisasini sp. nov. and streptococcus dentasini sp. nov., isolated from the oral cavity of donkeys. | four gram-positive, catalase-negative, coccoid isolates that were obtained from donkey oral cavities formed two distinct clonal groups when characterized by phenotypic and phylogenetic studies. from the results of biochemical tests, the organisms were tentatively identified as a streptococcal species. comparative 16s rrna gene sequencing studies confirmed the organisms to be members of the genus streptococcus. two of the isolates were related most closely to streptococcus ursoris with 95.6 % sim ... | 2013 | 23291897 |
| characterization of streptococcus criceti insertion sequence isscr1. | a novel insertion sequence element of the is982 family, isscr1, was previously identified in streptococcus criceti strain e49 as a disrupted paab gene encoding an antigen i/ii homologous protein. in this study, we identified two divergent inserted regions of isscr1 in s. criceti e49 by inverse polymerase chain reaction, the gene-walking method, and screening of the partial plasmid library. nucleotide sequence analysis indicated the possible explanation that transposition generated 8- and 9-bp di ... | 2012 | 22976390 |
| fluoride inhibits the glucan-binding lectin of streptococcus sobrinus. | the glucan-binding lectins of streptococcus cricetus aht and streptococcus sobrinus 6715 were reversibly inhibited by sodium fluoride. fluoride was superior to chloride, bromide, iodide and thiocyanate in preventing glucan-mediated aggregation of the bacteria. fluoride was also an effective inhibitor of the sucrose-dependent adhesion of s. sobrinus to glass surfaces. the inhibition of glucan-binding lectin activities may be one of the mechanisms of action of fluoride in preventing dental disease ... | 1994 | 7988914 |
| comparison of culture media and chairside assays for enumerating mutans streptococci. | this study compared several traditional culture-based media and chairside cultural assays for ability to recover mutans streptococci (ms) from pure cultures and from saliva samples. | 2006 | 16696682 |
| differentiation of mutans streptococci by intact cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. | it is difficult to distinguish mutans streptococci on the species level, and even more so on the subspecies level. intact cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof-ms) (icm) was applied to reference strains of five of the species of the mutans group (streptococcus criceti, streptococcus downei, streptococcus mutans, streptococcus ratti, streptococcus sobrinus), nonmutans streptococci (streptococcus oralis, streptococcus mitis, streptococcus sali ... | 2005 | 16101961 |