extensive gas in tissues of the forearm after horsebite.a 44-year-old man sustained lacerations of the forearm as a result of a horsebite. his arm became swollen after primary closure of the wounds, and a roentgenogram showed gas in the tissues of the forearm. streptococcus anginosus and s mutans were isolated from the wounds.1979505087
biological characterisation of group f streptococci causing human infections.fifty five strains of group f streptococci (gfs) isolated from clinical samples of 53 patients with diverse diseases, were characterised by a battery of physiological tests. among them 37 (67%) were streptococcus anginosus f, 14 (25%) were strep. intermedius, two of which were mannitol positive and 4 (7%) were strep. constellatus. determination of minimum inhibitory concentrations of penicillin, erythromycin, ampicillin, gentamicin and cefazolin showed that despite their biochemical heterogeneit ...19921506062
streptococcus milleri group: renewed interest in an elusive pathogen.the following review examines the bacteriological characteristics, epidemiology, pathogenicity and antimicrobial susceptibility of the "streptococcus milleri group". "streptococcus milleri group" is a term for a large group of streptococci which includes streptococcus intermedius, streptococcus constellatus and streptococcus anginosus. usually considered commensals, these organisms are often associated with various pyogenic infections including cardiac, abdominal, skin and central nervous system ...19921526231
[fatal streptococcus anginosus-milleri (lancefield group f) meningitis in a cocaine addict]. 19921552786
group c streptococci: "streptococcus equisimilis" or streptococcus anginosus? 19921554852
infectious arthritis in patients with rheumatoid arthritis.eleven cases of infectious arthritis occurring in patients with rheumatoid arthritis are reported. staphylococcus aureus was the causative organism in eight patients. streptococcus anginosus and streptococcus agalactiae in one patient each, and mycobacterium tuberculosis in two patients. the mean duration of symptoms before diagnosis was 16 days in patients with pyogenic arthritis. the diagnosis of joint infection caused by mycobacterium tuberculosis was especially delayed (57 days). four patien ...19921575593
[osteomyelitis around a knee prosthesis due to streptococcus anginosus]. 19921579710
bactericidal titers of loracarbef (ly 163892) in serum and killing rates in volunteers receiving 400 versus 200 a randomized crossover trial, six volunteers received 200- and 400-mg doses of loracarbef (ly 163892), a new oral cephalosporin. mean +/- standard error of the mean concentrations in serum obtained after 1.5 and 3 h were 13.2 +/- 2.8 and 4.3 +/- 0.7 mg/liter, respectively, after the 400-mg dose and 6.9 +/- 1.0 and 1.7 +/- 0.2 mg/liter, respectively, after the 200-mg dose. bactericidal reciprocal titers measured against respiratory pathogens in serum suggested that loracarbef would be highly e ...19921622160
streptococcus intermedius, streptococcus constellatus, and streptococcus anginosus (the streptococcus milleri group): association with different body sites and clinical infections.the associations of streptococcus intermedius, s. constellatus, and s. anginosus (the three species of the s. milleri group) with clinical infections and sites of isolation were investigated by using a simple biochemical scheme to identify a collection of 153 clinical isolates. s. intermedius was associated with abscesses of the brain and liver, while both s. anginosus and s. constellatus were isolated from a wider range of sites and infections. s. anginosus strains predominated in both genitour ...19921734062
[studies on clinical significance of "streptococcus milleri group" in respiratory infections]."streptococcus milleri group" are the part of the indigenous oral flora, and they are proposed to contain three distinct species: streptococcus anginosus, streptococcus constellatus, and streptococcus intermedius. though not included in the approved lists of bacterial names, "s. milleri group" are regarded as the causative organisms of suppurative infections, such as oral abscess, brain abscess, lung abscess and empyema. i have studied the clinical significance of the "s. milleri group" in respi ...19911791343
a modified mitis salivarius medium for a caries diagnostic test.a new medium, mskb, composed of mitis salivarius agar base, sorbitol, kanamycin sulfate and bacitracin, has been developed that is more selective for recovery of mutans streptococci (mutans) than the so called mitis salivarius bacitracin (msb) medium. msb and mskb were compared for recovery and selectivity of mutans by plating saliva samples as well as pure cultures on both media. sixty saliva samples were plated and counted for mutans and non-mutans colonies. thirty-six samples had greater numb ...19911820563
ecology of viridans streptococci in the oral cavity and pharynx.recently published taxonomic studies of viridans streptococci have resulted in several changes in the nomenclature and definition of oral streptococcal species. with this background, the ecology of streptococci in the oropharyngeal cavities was reinvestigated. the results based on the examination of 1426 streptococcal isolates confirmed and extended earlier findings. apart from mature supragingival plaque, which contained a mixture of all orally encountered streptococci, each site showed a chara ...19911945494
emended descriptions and recognition of streptococcus constellatus, streptococcus intermedius, and streptococcus anginosus as distinct species.strains currently classified as streptococcus anginosus include strains previously identified as streptococcus constellatus (prevot 1924) holdeman and moore 1974, streptococcus intermedius (prevot 1925), and "streptococcus milleri" (guthof 1956) because these specific epithets were argued to be later synonyms of streptococcus anginosus (andrewes and horder 1906) smith and sherman 1938 by coykendall et al. (int. j. syst. bacteriol. 37:222-228, 1987). however, recent data from dna-dna hybridizatio ...19911995029
effects of chlorhexidine on proteolytic and glycosidic enzyme activities of dental plaque bacteria.chlorhexidine was tested for its ability to inhibit a wide range of glycosidic and proteolytic enzyme activities produced by treponema denticola, porphyromonas gingivalis, bacteroides intermedius, actinobacillus actinomycemcomitans, capnocytophaga sputigena, capnocytophaga gingivalis, capnocytophaga orchracea, capnocytophaga sp., actinomyces viscosus, streptococcus mitior, streptococcus mutans, streptococcus sobrinus, streptococcus mitis, streptococcus anginosus, streptococcus oralis and strepto ...19912005230
streptococcus milleri group (streptococcus anginosus): recovery from intra-abdominal and soft tissue hundred thirty-three streptococcus milleri group (s. anginosus) isolates were recovered from 487 surgical patients. the streptococci were recovered from 33 percent of intra-abdominal infection cultures (84/257). 22 percent of samples from penetrating visceral trauma (19/86), 52 percent of perirectal abscess specimens (13/25), 13 percent of nonpuerperal breast abscess cultures (8/60), and 15 percent of diabetic foot lesions (9/59). ninety-eight percent of the s. milleri (131/133) were recover ...19912012380
serogrouping of oral streptococcus intermedius.employing twenty fresh oral isolates of streptococcus intermedius, studies were carried out to characterize serological relations among the isolates and also between the isolates and the strains of bacterial species closely related to s. intermedius. the rantz-randall extracts from the cells were used as antigens. the anti-rabbit serum raised against s. intermedius atcc 27335t reacted with the cell extracts from only three strains of the isolates, which were designated serogroup i strains. the o ...19902111871
immunochemical characterization of type i carbohydrate antigen of "streptococcus milleri" (streptococcus anginosus).type-specific carbohydrate antigen of the serotype i "streptococcus milleri" was extracted with trichloroacetic acid from purified cell walls of the type strain k39k. the extracts were then purified by chromatography on deae-sephadex a-25 and sephadex g-100 columns. the purified serotype i carbohydrate antigen produced a single precipitin band against its homologous type-specific antiserum, which fused with the band produced by the autoclaved extract of the type strain cells. the serotype i anti ...19902124490
coaggregation of streptococcus sanguis and other streptococci with candida albicans.thirteen strains of viridans group streptococci and two strains of other streptococci were tested for coaggregation with candida albicans. streptococcus sanguis strains generally exhibited low levels of adherence to 28 degrees c-grown exponential-phase yeast cells, but starvation of yeast cells for glucose at 37 degrees c (or at 28 degrees c) increased their coaggregating activity with these streptococci by at least tenfold. this was a property common to four c. albicans strains tested, two of w ...19902182544
group c streptococcal arthritis: case report and review.streptococci account for approximately 15%-20% of cases of nongonococcal septic arthritis. the majority of these are due to group a streptococci, but group b and group g streptococci are being isolated more frequently. we present a case of group c streptococcal arthritis and summarize nine additional cases reported in the literature. the group c streptococci include the large colony of voges-proskauer-negative bacteria (streptococcus equi, streptococcus equisimilis, streptococcus zooepidemicus, ...19902237126
ability to bind salivary alpha-amylase discriminates certain viridans group streptococcal species.a collection of 144 viridans group streptococcal strains recently characterized as part of a taxonomic study was examined for the ability to bind salivary alpha-amylase. this property was found in most strains of streptococcus gordonii and streptococcus mitis and in occasional strains of streptococcus anginosus and streptococcus salivarius. in contrast, all strains of streptococcus sanguis, streptococcus oralis, streptococcus vestibularis, and streptococcus mutans lacked alpha-amylase-binding ca ...19902254435
applications of bacteriocin-like inhibitory substance (blis) typing in a longitudinal study of the oral carriage of beta-haemolytic streptococci by a group of dunedin schoolchildren.a longitudinal study of the distribution of oral beta-haemolytic streptococci in 103 children (ages 5-6 y) attending three dunedin schools was initiated in february 1987. a total of 858 paired pharyngeal and saliva specimens were obtained in nine principal sampling sessions, the last being in may 1989. the detection rate of lancefield group a streptococci in directly plated pharyngeal cultures (19.8%) was considerably higher than in the corresponding saliva cultures (5.1%). by contrast, lancefie ...19902261062
identification of chromosomal antibiotic resistance genes in streptococcus anginosus ("s. milleri").determinants encoding resistance to antibiotics, except penicillin, of 5 of 21 streptococcus anginosus clinical isolates that we examined transferred by conjugation into enterococcus faecalis and into 1 to 5 streptococcal recipients at frequencies of 10(-6) to 10(-8) transconjugants per donor. no r plasmids were detected in any wild-type strain. dna homology was detected between chromosomal fragments and the following genes: aade in 7 strains that were highly resistant to streptomycin, aph-3 in ...19902285280
phenotypic differentiation of streptococcus intermedius, streptococcus constellatus, and streptococcus anginosus strains within the "streptococcus milleri group".a biochemical scheme was developed by which strains of streptococcus constellatus, streptococcus intermedius, and streptococcus anginosus can reliably be distinguished from within the "streptococcus milleri group." strains identified as s. intermedius were differentiated by the ability to produce detectable levels of alpha-glucosidase, beta-galactosidase, beta-d-fucosidase, beta-n-acetylgalactosaminidase, beta-n-acetylglucosaminidase, and sialidase with 4-methylumbelliferyl-linked fluorogenic su ...19902380375
the epidemiology of beta-haemolytic non-group a streptococci isolated from the throats of children over a one-year period.the incidence of beta-haemolytic non-group a streptococci (bhnas) in the throats of a paediatric population was examined over a 1-year period. there was minimal seasonal fluctuation of lancefield groups including species and biotypes within groups c and g streptococci. a trend of increasing incidence with age of streptococcus anginosus ('streptococcus milleri') (possessing groups c and g lancefield antigens) was evident. a clinical impression of streptococcal pharyngitis was more common in patie ...19902407542
a qualitative and quantitative study of the cellular fatty acids of 'streptococcus milleri' with capillary gas chromatography.fatty acid analysis was done with gc and gc-ms on 21 strains of 'streptococcus milleri', representative of the various proposed species. although no qualitative differences were found in the fatty acid profiles, discriminant analysis of the quantitative data revealed three groups. streptococcus anginosus and streptococcus constellatus were indistinguishable but separated from the other two groups which comprised streptococcus intermedius, with a wide fermentation pattern and streptococcus interm ...19892600587
[taxonomic studies of the streptococcus intermedius strains isolated from human oral cavities].human oral isolates of streptococcus intermedius, which were biochemically identified, have been classified into 5-serogroups (i-v). selecting some representive stock cultures together with the type strain atcc 27335, comparisons were carried out between them in terms of cell extractable proteins, g + c mol% values, dna-dna homology and dna restriction fragments. since the taxon of s. intermedius has not been clarified, comparison was also made between the above strains and the type strains of t ...19892610299
dna-dna hybridization studies and phenotypic characteristics of strains within the 'streptococcus milleri group'.twenty-five strains resembling 'streptococcus milleri' were compared by dna-dna hybridization, by whole-cell-derived polypeptide patterns on sds-page, and by biochemical tests. four homology groups were revealed by dna-dna hybridization. dna homology groups 1, 2 and 3 were closely related and contained the type strains ncdo 2226 (streptococcus constellatus), ncdo 2227 (streptococcus intermedius) and nctc 10713 (streptococcus anginosus), respectively. dna homology group 4 consisted of four strain ...19892632666
septic arthritis of the knee due to streptococcus anginosus.a case of arthritis caused by streptococcus anginosus was observed in which the only predisposing factor was osteoarthritis of the knee and an acute meniscal tear. we report here the fifth case of septic arthritis due to streptococcus anginosus.19892752717
streptococcus anginosus ("streptococcus milleri"): the unrecognized pathogen."streptococcus milleri" is an unofficial name that has been applied to a group of streptococci which, although basically similar, show various hemolytic, serological, and physiological characteristics. the species name streptococcus anginosus has recently been recognized as the approved name for these organisms. streptococci known as "s. milleri" have been implicated as etiologic agents in a variety of serious purulent infections, but because of their heterogeneous characteristics, these organis ...19883060239
clinically significant streptococcus anginosus (streptococcus milleri) infections: a review of 186 cases.we describe clinically significant infections due to streptococcus anginosus in 186 patients; 114 (61.3%) males and 72 (38.7%) females, median age 42 years, range 9 months to 93 years. in 101 (54.3%) cases s anginosus alone caused infection and in 85 (45.7%) cases it was associated with other microorganisms. abscesses accounted for 110 (59.1%) infections. sites of infection were: miscellaneous skin and soft tissue, 64 (34.4%), intraabdominal 41 (22%), head and neck 34 (18.3%), pleuropulmonary 22 ...19883060769
[a case of meningoencephalitis caused by group f streptococcus anginosus]. 19873112291
do the beta-hemolytic non-group a streptococci cause pharyngitis?implication of the beta-hemolytic non-group a streptococci (bhnas) as pharyngeal pathogens has been based predominantly on reports of a few outbreaks, small case clusters, and anecdotes. these organisms have long been noted to constitute a significant number of the beta-hemolytic streptococcal isolates from throats of symptomatic and asymptomatic patients in a variety of populations. laboratory studies have demonstrated the usefulness of anaerobic atmosphere and prolonged incubation in maximizin ...19883293161
fatty acid composition of streptococcus milleri.the cellular fatty acids of 31 strains belonging to the streptococcus milleri group were analysed by capillary gas-liquid chromatography. results were compared with findings from biochemical differentiation of the strains into streptococcus constellatus (two strains), streptococcus anginosus (16 strains) and streptococcus intermedius (13 strains). eight strains of various other streptococci were included as internal references, including three strains of streptococcus morbillorum, three strains ...19854043056
[clinical evaluation of ceftizoxime in the pediatric infections (author's transl)].ceftizoxime (fk 749, czx) was evaluated in 24 children with a suspicion of bacterial infection. of the 17 confirmed bacterial infections, 16 were shown to be effective (effective rate, 94.1%). the diagnosis included acute pharyngitis (2), pneumonia (6), staphylococcal empyema (1), cervical purulent lymphadenitis (2), acute enterocolitis (2), acute pyelonephritis (1), ssss (1) and suspected septicemia (2). the etiological pathogens recovered were streptococcus anginosus (1), streptococcus pneumon ...19826279902
streptococcus anginosus-constellatus infection of the sternoclavicular joint.we describe the first reported instance of sternoclavicular pyarthrosis caused by streptococcus anginosus-constellatus. infections caused by this organism are unusual in a nonimmunocompromised host. common conditions predisposing to sternoclavicular joint infection were not present. a possible mechanism for the infection is offered.19846392552
prevalence of viridans streptococci exhibiting lactose-inhibitable coaggregation with oral actinomycetes.fresh oral isolates from human dental plaque were selected on the basis of their spherical morphology. in a double-blind experiment, their species identity and ability to coaggregate with human oral actinomyces viscosus and actinomyces naeslundii were determined. of the 110 isolates characterized, 30 were identified as either streptococcus mutans, streptococcus anginosus-constellatus, or veillonella parvula; none of these coaggregated with the actinomycetes. the remaining 80 isolates were identi ...19836409806
purulent pericarditis caused by streptococcus anginosus-constellatus. 19846611499
[streptococcus anginosus deficient endocarditis. manifestation by rupture of a cerebral mycotic aneurysm]. 19826897675
streptococcus anginosus-constellatus infections of the nervous system.two patients are presented with central nervous system infections due to an uncommon type of viridans streptococci, s anginosus-constellatus. of the various streptococci, s pneumoniae is the most common etiologic agent in meningitis, but many species, including those which are facultative anaerobes or microaerophiles, have been isolated from cerebrospinal fluid, subdural empyema, and abscesses involving the brain and epidural space.19827079822
[septicemia and multiple abscesses of the liver caused by streptococcus anginosus]. 19827110976
bacteriology of experimental gingivitis in young adult humans.from replicate trials of experimental gingivitis in four periodontally healthy subjects, 166 bacterial species and subspecies were detected among 3,034 randomly selected isolates from 96 samples. of these bacteria, actinomyces naeslundii (serotype iii and phenotypically similar strains that were unreactive with available antisera), actinomyces odontolyticus (serotype i and phenotypically similar strains that were unreactive with available antisera), fusobacterium nucleatum, lactobacillus species ...19827141708
"streptococcus milleri" strains displaying a gliding type of motility.isolates belonging to the "streptococcus milleri" species group that appear to exhibit a gliding type of motility, which is expressed as spreading growth on certain types of agar media, are described. these strains resembled a biotype of "s. milleri" that is usually isolated from genitourinary sources and is notable for its ability to ferment a wide array of carbohydrates. this biotype, which is currently included in the species streptococcus anginosus, has been implicated in cases of neonatal i ...19957537057
heterogeneity among 16s-23s rrna intergenic spacers of species within the 'streptococcus milleri group'.the 16s-23s rrna intergenic spacer has been suggested as a suitable region of the bacterial genome from which to derive useful taxonomic information, particularly with regard to identification at the species level. to investigate this approach as an aid to the identification of the three species comprising the 'streptococcus milleri group' (smg), the spacers of isolates of streptococcus intermedius, streptococcus anginosus and streptococcus constellatus were amplified by pcr and length polymorph ...19957545512
streptococcus anginosus, streptococcus constellatus and streptococcus intermedius. clinical relevance, hemolytic and serologic characteristics.a collection of 518 "streptococcus milleri" isolates recovered from clinical specimens was identified to the species level according to recently established criteria. streptococcus anginosus was the most frequently isolated species (59.3%), followed by s constellatus (30.3%) and s intermedius (10.4%). one third (34%) of all isolates were beta-hemolytic. the majority of s anginosus isolates were nonhemolytic, carried lancefield group f, or were nongroupable. most of the s constellatus isolates we ...19957572815
presence of streptococcus dna sequence in surgical specimens of gastric southern blot analysis using mycoplasma 16s ribosomal dna (rdna) as a probe, positive signals were detected in dna samples from surgical specimens of gastric cancers. the dna that hybridized to mycoplasma 16s rdna was eluted from the gel, cloned and sequenced. the cloned sequence was identical to 16s rdna of streptococcus anginosus. in southern blot analysis with the s. anginosus 16s rdna fragment as a new probe, positive signals were detected in 9 (20%) out of 43 cases of gastric cancer.19957591953
species identification of members of the streptococcus milleri group isolated from the vagina by id 32 strep system and differential phenotypic characteristics.the importance of bacterial vaginosis as a risk factor in obstetric and gynecological infections has recently been recognized. the bacterial vaginosis group of organisms includes members of the streptococcus milleri group, the identification of which has caused much confusion. we prospectively surveyed the rates of carriage of s. milleri group organisms in 397 high vaginal swabs received in our laboratory. for the identification of 99 clinical isolates and 23 control strains, we compared the res ...19957650193
[fulminant fasciitis in the head and neck region].deep interstitial infections of the head and neck are potentially life-threatening conditions because of the double threat of upper airway obstruction and production of bacterial toxins, which may result in spread of infection, sepsis and multiorgan failure. at an early stage the symptoms are rather diffuse. it is of critical importance however, to make an early diagnosis if a fatal outcome is to be prevented. we describe a patient with infection caused by group f streptococci, (streptococcus an ...19957676416
bacteremia involving the "streptococcus milleri" group: analysis of 19 cases.during a 3-year study period, 19 patients at the university hospital of maastricht developed bloodstream infections with species of the "streptococcus milleri" group, for an incidence of 0.33 per 1,000 admissions. the patients' median age was 48 years; the male-to-female ratio was 2.8. eleven patients (57.9%) had underlying diseases, among which malignancy was predominant. local trauma to the mucosal barrier was an important risk factor. an associated site of infection was found most frequently ...19947803636
biological properties of irm with the addition of hydroxyapatite as a retrograde root filling material.the effect of adding 10% & 20% hydroxyapatite (hap) on the antibacterial activity and cytotoxicity of irm (intermediate restorative material) when used as a retrograde root filling was compared with amalgam, a commonly used material. the antibacterial activity was assessed using the agar diffusion inhibitory test. forty standardized pellets of each material were produced. fresh materials, and materials aged for 1 week in sterile distilled water, were placed on blood agar plates inoculated with s ...19947843065
[parenchymatous abscess in streptococcus anginosus (streptococcus milleri) septicemia. value of their systematic search, apropos of 4 cases].streptococcus anginosus, the term suggested to cover a set of streptococci previously known under various names (milleri, mg, anginosus, intermedius, constellatus), is characterized by a propensity to create parenchymatous abscesses, essentially cerebral or hepatic, particularly within the terms of septicemia. these abscesses are sometimes difficult to detect due to a difficult or non-existent symptomatology. the authors report on four cases illustrating the necessity to search for them systemat ...19947846385
mucin-sulphatase activity of some oral streptococci.mucin-sulphatase activity, measured using a 35s-[so4(2-)]-labelled colonic mucin substrate, was detected in whole cells of streptococci isolated from the human oral cavity. the highest levels of sulphatase activity were found in all strains of streptococcus salivarius, streptococcus mitis and in half of the strains of streptococcus mutans tested. little or no activity was detected in 9 of the 11 streptococcus oralis strains examined, in the 4 streptococcus constellatus strains, and in the 3 stre ...19947850844
the antimicrobial activity of prevention mouthrinse.prevention mouthrinse was designed to serve as a vital supplement to normal oral hygiene procedures. to determine the antimicrobial potency of this mouthrinse, minimal inhibitory concentrations (mics), bactericidal kinetics, and short-term exposure studies were conducted. a spectrum of oral microorganisms was employed in this investigation: streptococcus sanguis, streptococcus anginosus, streptococcus mutans, actinomyces viscosus, actinobacillus actinomycetemcomitans, fusobacterium nucleatum, ei ...19937880467
bacterial retention in canal walls in vitro: effect of smear layer.when dentin is planed by endodontic instruments, a smear layer forms. whether this layer should be removed is unknown and controversial. this study was conducted to assess the effect of the smear layer on retention of bacteria using an in vitro root canal bacterial colonization model. canals of 26 extracted human canines were step-back prepared using 2.5% naocl. teeth were then randomly divided into two groups based on the type of high volume final flush: 1-20 ml of sterile saline (0.85% wt/vol) ...19948006570
antibacterial activity of potential retrograde root filling materials.the antibacterial activity of potential retrograde root filling materials was assessed using the agar diffusion inhibitory test. a light-cured glass ionomer cement (vitrebond) and three reinforced zinc oxide-eugenol cements (kalzinol, irm & ethoxybenzoic acid [eba] cement) were compared with amalgam, a commonly used retrograde root filling material. forty standardized pellets of each material were produced. fresh, and materials aged for 1 week in sterile distilled water, were placed on blood aga ...19948062809
dog-associated bacterial infections in humans: isolates submitted to an australian reference laboratory, 1981-1992.over the period 1981-92, 32 bacterial isolates were referred to the microbiological diagnostic unit from infected dog-bite wounds and 10 isolates were submitted from blood cultures after dog bites or close contact with dogs. the isolates from the bite wounds were identified, or confirmed, as pasteurella multocida (11 isolates), pasteurella dagmatis (3), cdc group m-5 (9), cdc group ef-4a (8) and streptococcus anginosus (1). five of the 9 patients from whom cdc group m-5 was cultured had mixed in ...19938165003
[evaluation of phenotype characteristics of streptococci in the identification of the streptococcus anginosus (streptococcus milleri) group].microorganisms referred to as streptococcus anginosus (streptococcus milleri) group which consist of s. anginosus, s. intermedius and s. constellatus is very difficult to identify to the species level, because of their diversity of biochemical, hemolytic and serological characteristics and because of confusion surrounding their taxonomy. identification procedures on three isolates from primary sterile sites of three different patients, using api 20a system are described. streptococcus intermediu ...19938170272
genetic and molecular studies of a composite chromosomal element (tn3705) containing a tn916-modified structure (tn3704) in streptococcus anginosus f22.the plasmid-free streptococcus anginosus f22 contained a conjugative element, tn3705, encoding resistance to erythromycin (emr) and tetracycline-minocycline (tcr-mnr). we mapped a chromosomal region (> 52 kb) of f22, corresponding to the internal part of tn3705. molecular analysis of tn3705 revealed it to be a composite structure: it included in its central part a transposon designated tn3704 (20.3 kb +/- 0.5 kb), which had a modified structure in comparison with that of tn916 and on which the e ...19948171124
[surgical-radiologic interventional treatment in liver abscess with streptococcus anginosus milleri].pyogenic liver abscess is a relatively rare disease, often concerning elderly patients in bad general condition and with underlying diseases. out of a retrospective study (1984-1991) of 44 patients (f 43%, m 57%) with pyogenic liver abscesses and 12 patients (f 17%, m 83%) with amebic liver abscesses we present 5 patients with multifocal lesions due to streptococcus anginosus milleri--a special group concerning therapy and course of disease. the therapy was high-dose i.v. antibiotic therapy in a ...19938226038
enzymatic differentiation and biochemical and serological characteristics of the clinical isolates of streptococcus angiosus, s. intermedius and s. constellatus.of the 29 'streptococcus milleri' strains tested, all thirteen streptococcus intermedius (dna homology group 2) strains but none of the thirteen streptococcus anginosus (group 1) strains produced beta-n-acetylglucosaminidase, beta-n-acetylgalactosaminidase, alpha-n-acetylneuraminidase, beta-galactosidase, alpha-glucosidase, and hyaluronidase. the three streptococcus constellatus (group 3) strains produced only the latter two. glycosidase production divided 274 clinical isolates into 103 s. angin ...19938295553
recent taxonomic changes for anaerobic gram-positive and selected gram-negative organisms.the strains formerly classified as streptococcus anginosus or "streptococcus milleri" have now been recognized as three distinct species, streptococcus anginosus, streptococcus constellatus, and streptococcus intermedius. streptococcus morbillorum has been transferred into the genus gemella. four new species within the genus peptostreptococcus were recently named. a recent addition to the genus clostridium is clostridium argentinense, which includes clostridium botulinum type g. two new species ...19938324113
[microbiological characteristics and spectrum of infections of 108 streptococcus anginosus isolated].the aim of the present study was to assess the key microbiologic characteristics aid in the identification of streptococcus anginosus in the laboratory and know the spectrum of clinical infections it produces.19938347702
the isolation and characterization of milleri group streptococci from dental periapical abscesses.reports of the isolation of streptococci from dental abscesses have shown an association of the "s. milleri" group with such lesions. there has been considerable confusion regarding the taxonomy of these organisms, but the milleri group has recently been reclassified into three distinct species: streptococcus anginosus, streptococcus constellatus, and streptococcus intermedius. in this study, 45 samples from dental periapical abscesses were examined. milleri group streptococci were isolated from ...19938360361
cloning and expression of the multiple sugar metabolism (msm) operon of streptococcus mutans in heterologous streptococcal hosts.the multiple sugar metabolism (msm) operon of streptococcus mutans is responsible for the uptake and metabolism of a variety of sugars. in order to further characterize the substrate specificities of the transport system, a 12-kb region of dna containing the entire msm operon was cloned, via a novel two-step integration strategy, into the chromosomes of two heterologous streptococcal strains, streptococcus gordonii challis and streptococcus anginosus is57, as well as the chromosome of a natural ...19938432594
osteomyelitis of the pubic symphysis due to streptococcus anginosus. a case unusual case of osteomyelitis of the pubic symphysis in an insulin-dependent diabetic is reported. the infection occurred after the insertion of a penile prosthesis and the organism was streptococcus anginosus. healing took place after wide excision and drainage, with penicillin given systemically.19938449625
role of beta-hemolytic group c streptococci in pharyngitis: incidence and biochemical characteristics of streptococcus equisimilis and streptococcus anginosus in patients and healthy controls.the biochemical characteristics and the isolation rates of the two lancefield group c streptococcal species (s. equisimilis and s. anginosus) from patients with pharyngitis and asymptomatic controls were compared. some 239 strains of beta-hemolytic group c streptococci were isolated from 1,480 patients (209 strains) with pharyngitis and 227 controls (30 strains). a total of 44 strains displayed broad hemolysis, were voges-proskauer test negative, and produced glucuronidase. some 72.7% of these s ...19938463390
role of group c beta-hemolytic streptococci in pharyngitis: epidemiologic study of clinical features associated with isolation of group c streptococci.all lancefield group c beta-hemolytic streptococci isolated over 12 months from college students with clinical pharyngitis and age-matched healthy controls were identified. clinical features of upper respiratory tract infection and pyogenic pharyngitis as well as colony counts were tabulated for each patient according to throat culture results. of 1,480 patients, lancefield group c streptococcus equisimilis was isolated from 45 (3%) patients and streptococcus anginosus ("streptococcus milleri") ...19938463391
pyomyositis: early detection and treatment.we describe a patient with crohn's disease and pyomyositis due to streptococcus anginosus ("milleri"). early recognition of pyomyositis during its presuppurative phase allowed for prompt antibiotic treatment and rapid resolution of the muscle infection without surgical drainage.19938478863
deoxyribonucleic acid relatedness and phenotypic characteristics of oral streptococcus milleri strains.strains of oral streptococcus milleri group were compared with the type strains of streptococcus anginosus, s. intermedius, and s. constellatus by dna-dna hybridization at 60 degrees c. of the 29 strains tested, twelve, twelve, and two strains were closely related to s. anginosus atcc 33397t, s. intermedius atcc 27335t, and s. constellatus atcc 27823t, respectively. generally, the strains classified in the s. anginosus group in dna homology were non-beta-haemolytic and belonged to api s. milleri ...19938502174
oral streptococcal colonization of infants.the objective of this study was to determine the prevalence and proportions of different streptococcal species among the streptococcal flora during infancy. a total of 60 oral samples were collected by oral swabbing of the buccal mucosa and alveolar ridges of 18 infants before tooth eruption and from buccal and lingual surfaces of teeth after tooth eruption. a total of 549 isolates on mitis salivarius agar were speciated, principally by recently revised biochemical criteria of kilian et al. stre ...19938510978
[liver abscess caused by streptococcus anginosus].pyogenic liver abscess is a relatively rare disease, often concerning elderly patients in bad general condition and with underlying diseases. we report the case of a 77-year-old female with gastric cancer and liver abscesses by streptococcus anginosus.19958534540
hydrolytic enzymes of streptococcus anginosus, streptococcus constellatus and streptococcus intermedius in relation to infection.a collection of 518 'streptococcus milleri' strains was tested for the presence of hydrolytic enzymes, and the results were related to the clinical significance of the strains. ribonuclease activity was equally distributed among the three species while hyaluronidase activity was linked to streptococcus intermedius and streptococcus constellatus. both enzymes were not significantly associated with infection-related strains. deoxyribonuclease and chondroitin sulfatase activity tended to be present ...19958536735
fibronectin binding by streptococcus milleri group strains and partial characterisation of the fibronectin receptor of streptococcus anginosus f4.the streptococcus milleri group were shown to bind fibronectin (fn) to their cell-surface and this binding increased the adhesion of cells to hydroxyapatite. the binding of fn to streptococcus anginosus f4 was studied in more detail. fn binding to bacterial cells increased the association of the bacteria with the polymorphonuclear leukocytes obtained from the peritoneal cavity of rats but did not increase killing of the bacteria. the cell-surface receptor was a protein of m(r) 14,000 which was r ...19958559042
viridans streptococci isolated from the bloodstream. relevance of species identification.over a 4-year-period, 104 isolates belonging to the viridans streptococci were recovered from the blood and identified to the species according to beighton and co-workers. streptococcus oralis was the species most frequently recovered from patients in the hematology unit [29 of 39 (74%)]. streptococcus mitis ranked second [seven of 39 (18%)]. both species were associated with oromucositis. isolates presently identified as s. oralis are mainly those previously identified as s. mitis or streptococ ...19958565415
identification of "streptococcus milleri" group isolates to the species level with a commercially available rapid test system.clinical isolates of the "streptococcus milleri" species group were examined by conventional methods and a rapid, commercially available method for the identification of these strains to the species level. the levels of agreement between the identifications obtained with the commercially available system (fluo-card milleri; key scientific, round rock, tex.) and conventional methods were 98% for 50 streptococcus anginosus strains, 97% for 31 streptococcus constellatus strains, and 88% for 17 isol ...19958567909
liver abscess caused by haemophilus paraphrophilus and streptococcus anginosus. 19958572383
cross-reactivity between human sialyl lewis(x) oligosaccharide and common causative oral bacteria of infective endocarditis.the expression of sialy-lewis(x) (sle(x); neu5ac alpha 2-3 gal beta 1-4) (fuc alpha 1-3) glcnac-r) on oral bacteria producing infective endocarditis was determined by a whole-cell enzyme linked immunosorbent assay and an immunoelectron microscopy using the well-characterized anti-sle(x) monoclonal antibody snh-3 (mab snh-3; igm class). mab snh-3 reacted strongly with whole cells of oral bacteria: streptococcus sanguis, streptococcus mutans, streptococcus mitis, streptococcus salivarius, streptoc ...19958589666
immunochemical characterization of the carbohydrate antigens of serotype k and lancefield group g "streptococcus milleri".carbohydrate antigens of the serotype k/lancefield group g "streptococcus milleri" were extracted by autoclaving whole cells of the type k reference strain streptococcus anginosus k214-2k. the type k and group g antigen molecules are separated from each other and partially purified by a deae-sephadex a25 column chromatography followed by a sephadex g-100 gel filtration. in the double diffusion and the immunoelectrophoresis, the type k and group g antigen preparations obtained yielded single band ...19968604251
[streptococcus anginosus hepatic abscess]. 19968657678
evaluation of the automicrobic system for the identification of streptococcus mutans.the performance of the automicrobic system with the vitek gram-positive identification card (biomérieux, france) in identifying strains of streptococcus mutans was studied. of 160 strains assayed, 72.5% were confirmed to be streptococcus mutans; the remainder were identified as other species of streptocci (streptococcus bovis, streptococcus uberis, streptococcus anginosus, streptococcus sanguis i and ii, streptococcus intermedius, and streptococcus constellatus).19958681990
[a case of infective aortic valve endocarditis with splenic abscess].a 47-year-old woman was admitted to our hospital with high fever, exertional dyspnea and left flank pain. echocardiography revealed vegetations attached to the aortic valve and moderate aortic regurgitation. streptococcus anginosus was identified by blood culture examination. abdominal ct scan showed a low density area in the spleen. the diagnosis of infective aortic valve endocarditis with splenic abscess was obtained. the splenectomy and the aortic valve replacement were performed simultaneous ...19968691684
in-vitro antimicrobial susceptibility of the "streptococcus milleri' group (streptococcus anginosus, streptococcus constellatus and streptococcus intermedius).a collection of 423 clinical "streptococcus milleri' strains was speciated and tested for susceptibilities to twelve antibiotics. only 1.4% of the strains were of intermediate susceptibility to penicillin. none of the strains exhibited high-level resistance to gentamicin. strains resistant to erythromycin, roxithromycin and clindamycin were found with a frequency of 2.6%, 2.4% and 2.4% respectively. doxycycline resistance was found in 5.7% of the strains and occurred most frequently in streptoco ...19968707750
comparative evaluation of bactec aerobic plus/f and septi-chek release blood culture media.the bactec 9240 (becton dickinson, sparks, md.) automated blood culture system is based on the continuous monitoring of co2 production by means of a fluorescent sensor attached to the bottom of culture vials. we compared the performance of the bactec aerobic plus/f medium to that of the septi-chek release medium (becton dickinson, sparks, md.), a manual biphasic blood culture system. sets consisting of bactec aerobic plus/f and septi-chek release vials inoculated with similar volumes (7 +/- 2 ml ...19968748287
the streptococcus milleri group as a cause of pulmonary infections.streptococci that colonize the mouth and upper respiratory tract tend to be considered harmless commensals. in 45 cases of acute pneumonia and/or pulmonary abscess and 25 cases of thoracic empyema, the predominant species recovered were anaerobic bacteria and the streptococcus milleri group, which encompasses the oral species streptococcus anginosus, streptococcus constellatus, and streptococcus intermedius. the isolation of most s. milleri organisms along with oral anaerobes indicated synergy b ...19958749672
rapid species identification of "streptococcus milleri" strains by line blot hybridization: identification of a distinct 16s rrna population closely related to streptococcus constellatus.a collection of 399 "streptococcus milleri" strains were identified to the species level by the use of a line blot assay. their pcr-amplified partial 16s rrna gene sequences were hybridized with species-specific 5'-biotinylated oligonucleotide probes homologous to the bp 213 to 231 regions of the 16s rrna gene sequences of the type strains streptococcus anginosus atcc 33397, streptococcus constellatus atcc 27823, and streptococcus intermedius atcc 27335. the hybridization results were compared w ...19968784576
recurrent brain abscesses in an hiv-positive patient with hereditary hemorrhagic telangiectasia and arteriovenous malformations of the lung.patients with hereditary hemorrhagic telangiectasia are at risk for the development of brain abscesses. the history of a 47-year-old man infected with the human immunodeficiency virus and with hereditary hemorrhagic telangiectasia is reported. within eight months, the patient presented twice with life-threatening cerebral abscesses at different sites. on both occasions, streptococcus anginosus was cultured from the abscess material. treatment consisted of parenteral antibiotics and neurosurgical ...19968793402
experimental salivary pellicles formed on titanium surfaces mediate adhesion of streptococci.the goal of this study was to characterize salivary components of titanium pellicles and to determine how experimental pellicles affect adhesion of several strains of streptococci to titanium surfaces. titanium experimental pellicles were formed by incubation of fresh human parotid or human submandibular-sublingual saliva on pure titanium beads. pellicle was recovered from the beads using sodium dodecyl sulfate buffer and was subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis ...19968803339
species belonging to the "streptococcus milleri" group: antimicrobial susceptibility and comparative prevalence in significant clinical specimens.the association between the three species belonging to the "streptococcus milleri" group and different sites of isolation was examined for 73 successive strains recovered from clinically significant, purulent infections. susceptibility testing was performed on 64 of these strains. the present study supports the association of particular species with different clinical sources. susceptibility data suggest that emerging penicillin resistance among streptococcus anginosus and streptococcus intermed ...19968818904
postantibiotic effect of penicillin on streptococcus anginosus.we studied the postantibiotic effect of penicillin g on bacterial growth of two strains of streptococcus anginosus by optical density readings of the cultures and by counting the numbers of viable cells. duration of the effect of the drug in concentrations equivalent to the mics after exposure for 2 h was 3.4 and 3.5 h. the production of streptococcal substances was examined during the postantibiotic phase. the antibiotic caused an increase in deoxyribonuclease and a decrease in both free and ce ...19968861872
epidemiologic evidence for lancefield group c beta-hemolytic streptococci as a cause of exudative pharyngitis in college students.the isolation rates of strains of group c beta-hemolytic streptococci from throat swab cultures of patients with exudative pharyngitis, the common cold, and healthy controls were compared. by using a cohort study design in a college health service, patients with exudative pharyngitis were retrospectively identified by description of tonsillar exudate on chart review. patients with rhinoviral infection were prospectively identified during a common cold study. healthy controls were prospectively r ...19978968872
streptococcus anginosus spondylodiskitis. 19978994762
comparative in-vitro activities of quinupristin-dalfopristin against gram-positive bloodstream isolates.the in-vitro activity of quinupristin-dalfopristin was compared with those of vancomycin, teicoplanin, erythromycin, clarithromycin, rifampicin, imipenem, meropenem, ciprofloxacin and sparfloxacin against 414 bloodstream isolates of gram-positive cocci. quinupristin-dalfopristin inhibited strains of streptococcus pyogenes and streptococcus agalactiae at 0.12 mg/l, methicillin- and/or erythromycin-resistant staphylococcus aureus and staphylococcus epidermidis at 0.5 mg/l, staphylococcus haemolyti ...19979301986
[bacteremias due to the streptococcus milleri group. an analysis of 18 episodes].to study the epidemiological and clinical characteristics of bacteremia caused by streptococcus milleri group streptococci (smg).19979304128
experimental endocarditis induction and platelet aggregation by streptococcus anginosus, streptococcus constellatus and streptococcus intermedius.a total of 18 'streptococcus milleri' strains including the atcc type strains of streptococcus anginosus, streptococcus constellatus and streptococcus intermedius were compared with streptococcus oralis atcc10557 for their ability to induce infective endocarditis in catheterized rats. three days after intravenous injection of 10(8) colony-forming units all 8 s. anginosus strains tested produced infective vegetations and bacteremia in almost all rats whereas 5 s. constellatus strains and the s. o ...19979322066
intra-specific diversity within streptococcus anginosus. 19979331672
[aerobic and anaerobic bacterial flora in chronic sinusitis in adults].the aim of the study was to analyse microbiologically samples obtained from 30 patients aged from 21 to 73 years treated for chronic sinusitis. aerobic bacteria only were isolated in 16 patients (53%), and anaerobic organisms only in 5 patients (17%). mixed aerobic and anaerobic isolates were recovered from 9 patients (30%). the isolated aerobic bacteria were as follows: streptococci from the species streptococcus salivarius, streptococcus anginosus, streptococcus group c, streptococcus sanguis, ...19979411078
[pyogenic infections with different locations caused by streptococcus anginosus alone or in association with anaerobic bacteria].isolation and identification of s. anginosus from pathologic products are possible to be realised in informed clinical laboratory, allowing so a better knowledge of these infections' incidences and on adequate treatment. the authors' report 11 cases of pyogenic infection caused by s. anginosus. five out of these 11 cases evaluated as mixed infections, s. anginosus being associated with anaerobic bacteria. 10 children hospitalised in surgery department of children hospital had infections with dif ...19959455370
determination of 23s rrna sequences from members of the genus streptococcus and characterization of genetically distinct organisms previously identified as members of the streptococcus anginosus group.the 23s rrna gene sequences of eight type strains of the genus streptococcus were determined. three species-specific probes for the three members of the streptococcus anginosus group and one group-specific probe for these three species were designed. the pcr-amplified 23s rrna gene sequences of 28 clinical strains were examined by hybridization with the four oligonucleotide probes. they were suspected to be members of the s. anginosus group by biochemical tests; however, only 21 strains (75%) hy ...19989465395
antibacterial action of honey on oral streptococci.the antibacterial properties of honey against medically important bacteria have been well documented but this information is not available for the oral bacteria and specifically for the oral streptococci. we determined the minimal inhibitory concentration (mic) of honey for oral streptococci. honey had a mic of 25 per cent (vol/vol) for the bacteria tested with the exception of streptococcus anginosus and streptococcus oralis which were inhibited by 17 per cent (vol/vol) and 12 per cent (vol/vol ...19949508952
[the frequency of streptococcus anginosus in the pharyngeal exudate from children and its differentiation from other beta-hemolytic streptococci].s. anginosus is a commensal of the oro-pharyngeal mucous membrane without any signification for the local pathology. ignoring the existence of beta-haemolytic colonies of this species, the risk to report the presence of some beta-haemolytic streptococci that actually belong to the normal flora exists. the antigenic identifications of beta-haemolytic streptococci maintain the confusion either, s. anginosus being able to react with specific sera anti group g, c or a. in our study, two identificati ...19959524670
whole-cell protein electrophoretic analysis of viridans streptococci: evidence for heterogeneity among streptococcus mitis hundred reference strains representing all species belonging to the different phylogenetic lineages of the viridans streptococci were examined by means of one-dimensional whole-organism protein electrophoresis. for most of the species examined, multiple strains characterized by dna-dna hybridization were included and, wherever described, representatives of different biochemical variants were analysed. most species were clearly differentiated. the data support the viewpoint that members of th ...19989542082
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