| assignment of the agent of tyzzer's disease to clostridium piliforme comb. nov. on the basis of 16s rrna sequence analysis. | the small-subunit rrna (16s rrna) sequence of tyzzer's bacillus (also known as "bacillus piliformis") was elucidated by using the polymerase chain reaction followed by reverse transcriptase sequencing. by using maximum-likelihood analysis, a phylogenetic tree was constructed from this and other 16s rrna sequences available from the first release of the ribosomal database project (g. j. olsen, r. overbeek, n. larsen, t. l. marsh, m. j. mccaughey, m. a. maciukenas, w.-m. kuan, t. j. macke, y. xing ... | 1993 | 7684241 |
| 16s ribosomal dna sequences of anaerobic cocci and proposal of ruminococcus hansenii comb. nov. and ruminococcus productus comb. nov. | the 16s ribosomal dna sequences of representative members of the family peptococcaceae were determined. the members of the family examined were divided into the following four phylogenetic groups: peptococcus niger atcc 27731t (t = type strain), the sarcina-peptostreptococcus anaerobius group, the ruminococcus-coprococcus group, and the peptostreptococcus group. peptococcus niger, the type species of the family, was not related to other members of the family. peptostreptococcus anaerobius atcc 2 ... | 1994 | 8123556 |
| phylogenetic analysis of ruminococcus flavefaciens, the type species of the genus ruminococcus, does not support the reclassification of streptococcus hansenii and peptostreptococcus productus as ruminococci. | the 16s rrna gene sequence of the type strain of ruminococcus flavefaciens, the type species of the genus ruminococcus, was determined by pcr direct sequencing. a comparative sequence analysis showed that r. flavefaciens is phylogenetically related to a small cluster (cluster iv of collins et al. [m. d. collins, p. a. lawson, a. willems, j. j. cordoba, j. fernandez-garayzabal, p. garcia, j. cai, h. hippe, and j. a. e. farrow, int. j. syst. bacteriol. 44:812-826, 1994]) of organisms which include ... | 1995 | 8590686 |
| ruminococcus hydrogenotrophicus sp. nov., a new h2/co2-utilizing acetogenic bacterium isolated from human feces. | a new h2/co2-utilizing acetogenic bacterium was isolated from the feces of a non-methane-excreting human subject. the two strains s5a33 and s5a36 were strictly anaerobic, gram-positive, non-sporulating coccobacilli. the isolates grew autotrophically by metabolizing h2/co2 to form acetate as sole metabolite and were also able to grow heterotrophically on a variety of organic compounds. the major end product of glucose and fructose fermentation was acetate; the strains also formed ethanol, lactate ... | 1996 | 8703194 |
| distribution of genes encoding the trypsin-dependent lantibiotic ruminococcin a among bacteria isolated from human fecal microbiota. | fourteen bacterial strains capable of producing a trypsin-dependent antimicrobial substance active against clostridium perfringens were isolated from human fecal samples of various origins (from healthy adults and children, as well as from adults with chronic pouchitis). identification of these strains showed that they belonged to ruminococcus gnavus, clostridium nexile, and ruminococcus hansenii species or to new operational taxonomic units, all from the clostridium coccoides phylogenetic group ... | 2002 | 12089024 |
| detection of blastocystis from stool samples using real-time pcr. | we developed a real-time lc pcr assay to detect a 152 bp sequence in an uncharacterized region of the blastocystis genome. the described assay detected 11 of 11 atcc strains of blastocystis from subtypes 1, 3, and 4. three of three stool samples from oregon and california military personnel that were negative for blastocystis by an ova and parasite test as well as a conventional pcr assay were positive for blastocystis using our real-time lc pcr assay. diagnosis of blastocystis infections using ... | 2008 | 18488250 |
| reclassification of clostridium coccoides, ruminococcus hansenii, ruminococcus hydrogenotrophicus, ruminococcus luti, ruminococcus productus and ruminococcus schinkii as blautia coccoides gen. nov., comb. nov., blautia hansenii comb. nov., blautia hydrogenotrophica comb. nov., blautia luti comb. nov., blautia producta comb. nov., blautia schinkii comb. nov. and description of blautia wexlerae sp. nov., isolated from human faeces. | phenotypic and phylogenetic studies were performed on 15 isolates of an unidentified gram-positive, anaerobic, non-sporulating coccobacillus-shaped bacterium isolated from human faeces. the novel organisms were catalase-negative, indole-negative and produced acetate and succinate as end products of metabolism. comparative 16s rrna gene sequencing demonstrated that the 15 isolates were highly related to each other and formed a hitherto unknown subline within the clostridial rrna cluster xiva. the ... | 2008 | 18676476 |
| [protease activity of microflora in the oral cavity of patients with periodontitis]. | microbial spectrum and non-specific as well as specific iga1 protease activity of isolated microorganisms were investigated in gingival liquid of patients with periodontitis. microorganisms from the gingival liqud of these patients belonged to conditional-pathogenic obligate and facultatively anaerobic bacteria. 24 strains of microorganisms have been identified. nonspecific proteolytic activity was found in the following microorganisms: actinomyces israelii, actinomyces naeslundii, aerococcus vi ... | 2008 | 19205430 |