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isolation and purification of flavobacterium alpha-1,3-glucanase-hydrolyzing, insoluble, sticky glucan of streptococcus mutans.studies were made on the physical and chemical properties of polysaccharides synthesized by cell-free extracts of streptococcus mutans, streptococcus sanguis, and streptococcus sp. and their susceptibilities to dextranases. among the polysaccharides examined, insoluble glucans were rather resistant to available dextranase preparations, and the insoluble, sticky glucan produced by s. mutans omz 176, which could be important in formation of dental plaques, was the most resistant. by enrichment cul ...1975370
ph changes during culture of human dental plaque streptococci on mitis-salivarius agar. 19768030
purification and some properties of free and cell-associated dextransucrase from streptococcus sanguis.dextransucrase of streptococcus sanguis occurred in cell-free and cell-associated forms. cell-free dextransucrase was purified by four successive chromatographies on bio-gel p 60, deae-cellulose, and bio-gel p 200 from the culture supernatant. the purification of cell-associated dextransucrase was made from the pellet of streptococcus sanguis culture. bacterial pellet was extracted with 1 m phosphate buffer (ph 6.0) and chromatographied by using an immunosorbent column. the two enzymes gave sing ...197611837
lactate formation at various ph levels by the wild strain of streptococcus mutans pk 1, its variant, and s sanguis. 197612197
plaque formation by streptococci in an artificial mouth and factors influencing colonization. 197718435
acid production from lycasin, maltitol, sorbitol and xylitol by oral streptococci and lactobacilli.the acid production from maltitol was compared with the acid production from hydrogenated starch hydrolysate (lycasin), sorbitol and xylitol by a number of oral strains and reference strains of streptococcus mutans, s. sanguis, s. salivarius, s. mitior, s. milleri, s. faecalis, s. faecium, s. avium, lactobacillus casei and l. salivarius. the polyols were added to a final concentration of 1.0% to two different basal media. incubation was performed at 37 degrees c for 7 days after which the ph was ...197721508
attachment of bacteroides melaninogenicus subsp. asaccharolyticus to oral surfaces and its possible role in colonization of the mouth and of periodontal pockets.this investigation examined the ability of cells of bacteroides melaninogenicus subsp. asaccharolyticus 381 to adhere to surfaces that might be important for its initial colonization of the mouth and its subsequent colonization in periodontal pockets. of 48 asaccharolytic strains of b. melaninogenicus, 47 agglutinated human erythrocytes, whereas none of 20 fermentative strains, which included reference cultures of the subspecies intermedius and melaninogenicus, were active. electron microscopy i ...197824002
factors affecting the aggregation of actinomyces naeslundii during growth and in washed cell suspensions.various factors affecting the aggregation of actinomyces naeslundii strain 12104 were studied. when the ph of glucose-supplemented growth medium fell below 5.5, the cells aggregated and formed microbial masses which tenaciously adhered to the culture vessels. when the organism was cultured in the same medium in the absence of glucose, maximum growth was reduced and the final culture ph values remained above 6.5, but the cells were more dispersed and nonadherent. adjusting the final ph of these c ...197830700
mechanism of coaggregation between actinomyces viscosus t14v and streptococcus sanguis 34.actinomyces viscosus t14v and streptococcus sanguis 34 coaggregate by a mechanism which is not inhibited by 1 m nacl, is dextran independent, requires calcium, is ph dependent with an optimum at ph 8.0 to 8.5, and appears to require the interaction of a protein or glycoprotein on a. viscosus with a carbohydrate on s. sanguis. the coaggregation is inhibited more than 80% by 0.01 m lactose, 0.02 m beta-methyl-d-galactoside, or 0.05 m d-galactose; inhibition of coaggregation was less than 10% in 0. ...197830701
interrelationships between lactobacilli and streptococci in plaque formation on a tooth in an artificial mouth. 197832164
enhanced transformability with heterospecific deoxyribonucleic acid upon removal of nascent ribonucleic acid from the streptococcus sanguis genome.treatment of streptococcus sanguis recipient cells with rifampin (rif) at the time of deoxyribonucleic acid (dna) addition was an effective means of reducing discrimination, that is, of causing an increase in the number of transformants induced by irreversibly bound heterospecific dna without significantly changing the number induced by bound homospecific dna. rif was unable to reduce discrimination when the recipient cells were rif resistant due to an altered ribonucleic acid (rna) polymerase. ...197937232
plaque formation in vitro by actinomyces viscosus in the presence of streptococcus sanguis or streptococcus mutans.actinomyces viscosus, growing on a tooth in the presence of sucrose, slowly produced a loosely-attached plaque, the ph being 6.1 after 120 h. when the tooth was inoculated simultaneously with a. viscosus and either streptococcus sanguis or streptococcus mutans, firmly-adherent plaque was quickly formed and the ph fell below 5 after 33 h with the former streptococcus and 24 h with the latter. a. viscosus disappeared from each mixed plaque by 120 h.197842006
bacterial aggregating activity in human saliva: simultaneous determination of free and bound cells.two new assays for saliva-mediated aggregation of oral bacteria have been developed, based on the use of [3h]thymidine-labeled cells. one assay separates free cells from aggregated cells by centrifugation through sucrose, whereas the other utilizes membrane filters (8 micrometers, nuclepore) to effect the separation. comparison of these assays with the turbidity method reveals that they are faster (x20 to 40) and require 10 times less saliva and bacteria. the aggregation of streptococcus sanguis ...197943286
chemotherapeutic effect of zinc on streptococcus mutans and rat dental caries. 197944465
competence-related increased enzyme release from streptococcus sanguis (wicky) cells.the ablity of competent and noncompetent streptococcus sanguis (strain wicky) cells to release enzymes to the environment was studied. both competent and noncompetent cells leaked the enzymes tested (aldolase, phosphatase and deoxyribonuclease), but the activities liberated from the competent cells were always roughly 2-fold higher than those released from noncompetent cells. this increased enzyme leakage from competent cells occured in all kinds of media and procedures employed. the leakage of ...197881592
streptococci and the human oral flora. 197883002
new areas arising from studies of secretory immunity. 197884518
fimbriae of actinomyces viscosus t14v: their relationship to the virulence-associated antigen and to coaggregation with streptococcus sanguis 34.1) fimbriae from a. viscosus t14v may be similar to those found on other bacteria. 2) the antigenic difference between virulent and avirulent a. viscosus t14 appears to be of a quantitative rather than a qualitative nature and is related to fimbriae and not to the cell wall polysaccharide. 3) coaggregation between a. viscosus t14v and s. sanguis 34 is mediated by fimbriae on the former which have specificity for beta-linked galactosyl residues.197884522
interactions between human serum proteins and oral streptococci reveal occurrence of receptors for aggregated beta 2-microglobulin.a total of 31 strains of oral streptococci representing streptococcus mutans, streptococcus sanguis, streptococcus mitior, streptococcus salivarius, and streptococcus milleri were tested for possible binding of human immunoglobulins g, g1, g2, g3, g4, a1, a2, m1, and m2 and haptoglobin, hemoglobin, fibrinogen, and aggregated beta 2-microglobulin. radiolabeled beta 2-microglobulin in aggregated form showed affinity for 20 of the 31 strains tested. binding activity for the protein was found in str ...197990015
penicillin tolerance in experimental streptococcal endocarditis. 197991047
activity of three 8-hydroxyquinoline derivatives against in vitro dental plaque.three 8-hydroxyquinoline derivatives, assessed using an in vitro preformed dental plaque model system, were differentially inhibitory for four plaque-forming microorganisms.197898105
characterization of a new group specific antigen of streptococcus sanguis. 197885601
deoxyribonucleic acid sequence common to staphylococcal and streptococcal plasmids which specify erythromycin resistance.plasmids from erythromycin-resistant staphylococcus aureus, streptococcus sanguis, and streptococcus faecalis show deoxyribonucleic acid sequence homology. the homologous sequences can be localized to specific restriction endonuclease fragments, which in the case of s. aureus plasmid pi258 involves a single fragment from either ecori or hindiii digest known to contain the erythromycin resistance determinant. complementary ribonucleic acid probes prepared from s. aureus plasmid pi258 and s. sangu ...1979110774
genital occurrence of oral microbiota.recent studies indicate that tonsillar gonococcal infection or colonization is fairly common. carriage rates of about 8% have been found. these studies also indicate that oro-genital contacts are common. since very little is known about the amount of oral microbiota transmitted to the genitals, we have studied the occurrence of oral streptococci and neisseria species in urethra and cervix. among 128 patients attending an std-clinic we found 10 carriers of oral streptococci, one streptococcus mit ...197882332
the isolation and partial characterization of a sulphated glycoprotein from human whole saliva which aggregates strains of streptococcus sanguis but not streptococcus mutans. 1979120733
effect of actinomyces viscosus on the establishment and symbiosis of streptococcus mutans and streptococcus sanguis in spf rats on different sucrose diets. 1975123171
immunogenic properties of the glucosyltransferase from streptococcus sanguis omz 9: kinetic study of inhibition by antibodies.an anti-glucosyltransferase serum was prepared against a pure enzyme preparation from streptococcus sanguis omz 9, which synthesized both soluble and insoluble dextran. sera, crude gamma globulins, and antibody fractions obtained after gel filtration on a bio-gel p200 column were used to study enzyme-antibody interactions. a strong inhibition of glucosyltransferase activity was obtained only with the purified antibody fraction. kinetics studies showed that the anti-glucosyltransferase antibodies ...197878900
scanning electron microscopy of heart-valve vegetations. 197771629
predominant cultivable microbiota in periodontosis. 1977138729
bacteriological studies of developing supragingival dental plaque. 1977138733
streptococcus sanguis and streptococcus salivarius in early plaque formation on plastic films. 1977143517
stability of the resident microflora and the bacteriocinogeny of streptococcus mutans as factors affecting its establishment in specific pathogen-free rats.the outcome of the experimental implantation of streptococcus mutans strains in humans and animals is unpredictable, and neither success nor failure can be explained. it seems logical to assume that, apart from dietary and host factors, the characteristics of the s. mutans strain involved and those of the resident plaque microflora are important in colonization. for example, previous work in this laboratory suggested that bacteriocin production accounts at least in part for the establishment of ...1979154472
factors affecting the stability of the resident dental plaque microflora of specific pathogen-free rats in relation to the ability to resist colonization by streptococcus mutans. 1979161701
effect of polymyxin b on liposomal membranes derived from escherichia coli lipids.the specificity of the action of polymyxin b was studied using liposomes as a model membrane system. liposomes prepared from total lipids of gram-negative bacteria escherichia coli, a mixture of purified e. coli phosphatidylethanolamine and cardiolipin and a mixture of phosphatidylethanolamine and phosphatidylglycerol, were extemely sensitive to polymyxin while those prepared from lipids of gram-positive bacteria streptococcus sanguis, lipids of sheep erythrocyte membranes, mixtures of egg lecit ...1975163098
comparative ultrastructure of selected oral streptococci: thin-sectioning and freeze-etching studies.the ultrastructure of streptococcus mutans, serotypes a-e, s. sanguis, s. mitis, and s. salivarius hht, were examined by the techniques of thin-sectioning and freeze-etching. the cell walls varied in width between 15 and 46 nm and were covered with an electron-dense fibrillar or fuzz layer. the cytoplasmic membrane was in close association with numerous mesosomes which were, in turn, either closely associated or in contact with the bacterial chromosome. in freeze-etch replicas, the outermost lay ...1976177163
superhelical dna in streptococcus sanguis: role in recombination in vivo.competent streptococcus sanguis treated with non-lethal doses of coumermycin a1 immediately before or after uptake of radioactive transforming dna were reduced in their capacity to yield transformants. this treatment did not alter bacterial ability to bind dna in dnase i-resistant form, nor did it prevent the single-stranded donor dna-recipient protein complexes formed upon uptake at the surface of the bacteria from translocating to chromosomal sites. inhibition of transformation by heterospecif ...197943456
extracellular glucans produced by oral streptococci. 1976180941
the purification and properties of dextransucrase from streptococcus sanguis atcc 10558.dextransucrase has been purified from the culture fluids of s. sanguis 10558 by a combination of hydroxylapatite, ion-exchange, and gel-filtration steps. two active proteins were isolated with specific activities approaching one order of magnitude higher than other preparations reported. the enzymes have mol. wt. on the order of 100 000 and exhibit ph optima between 5,8 and 6.2. in addition, detailed analysis of one of the enzymes indicates that the enzyme undergoes two ionizations that are impo ...197939674
cell surface located dna binding factor occurring in transformable streptococcus sanguis strain wicky. 1979232423
the role of transamination in ammonium assimilation in streptococcus sanguis. 1975238495
soluble macromolecular complexes involving bacterial teichoic acids.cell wall and membrane teichoic acids from several bacteria formed soluble complexes with polysaccharides and bovine plasma in alkyl alcohol solutions. polysaccharides which contain different monomeric units and anomeric configurations complexed with the teichoic acids, suggesting that the interaction is relatively nonspecific. teichoic acids complexed glycogen or bovine plasma albumin in 50 to 97% ethanol solutions. the macromolecular association between teichoic acids and polysaccharides or pr ...1975240807
assay and properties of iga protease of streptococcus sanguis.an assay procedure for streptococcal iga protease is described which uses isotopically labelled human serum iga as substrate. enzyme activity was monitored by the radioactive counts in the fab alpha product, which was separated from other components in the digestion mixture by electrophoresis. cleavage of iga was linear with respect to time using catalytic amounts of the enzyme. km was calculated to be 5.5 x 10(-6)m, ph optimum 6.0-7.0 at 37 degrees c, and the enzyme was fully inactivated at low ...197833542
studies on the bacterial components which bind streptococcus sanguis and streptococcus mutans to hydroxyapatite. 1975241311
enchancement of streptococcal transformation yield by proteolytic enzymes.trypsin and other proteolytic enzymes, added together with transforming dna or during cell-dna contact to competent cultures of several streptococcal strains, enchanced (10 to 600%) the yield of genetic transformation (stimulation). with few exceptions, the level of stimulation was high (over 100%) when competence was low (below 2%). stimulation was caused by the action of an enzyme on competent cells and not on any other component of transformation mixture. the phenomenon occurred when the enzy ...197830748
infective endocarditis: part ii. current therapy. 1978249230
human saliva as a nitrogen source for oral streptococci. 1977264243
competitive binding among oral strptococci to hydroxyapatite.the relative affinity of various oral streptococci for hydroxyapatite (ha) differed widely. streptococcus mutans affinity for dextran-coated ha was the highest of all the streptococci to any ha coating. s salivarius had the lowest affinity, and s sanguis affnity was higher then s miteor for saliva-coated ha. competition for binding sites on saliva-dextran-coated, dextran-coated ha. hhwever, competition between this pair was not for the same binding site but for closely associated sites.1977264883
interaction of streptococcal aggregating factors with thiol-and disulphide-reactive compounds. 1977266858
bacteriocin-like activities of human dental plaque flora against oral anaerobic microorganisms. 197726477
specific inactivation of heterospecific transforming dna by a factor derived from streptococcus sanguis lysates.a heat-sensitive factor obtained from lysates of competent streptococcus sanguis cells reacts specifically with native dna of heterospecific (s. pneumoniae or calf thymus) origin. in vitro it does not alter the double or single strand length of the dna, nor does it affect uptake of the dna by compentent s. pneumoniae cells in dnase i-resistant form. following uptake, however, dna previously exposed to the factor loses over 90% of its biological activity. reaction of heterospecific dna with the f ...197826019
microbial characterization of an experimental cariogenic plaque in man.experimentally induced plaque seemed to originate by direct contact inoculation from the vestibular mucosa and saliva. during the next seven days, this plaque developed its own characteristics. populations of streptococcus mutans usually less than 2% of total streptococci population in plaques that were less than three days old, increased between days 3 and 7. proportions of s sanguis, high in early samples, decreased after day 3. populations of s salivarius, which usually outnumbered other stre ...1977268335
comparison of antiplaque agents using an in vitro assay reflecting oral conditions.an in vitro assay is described using saliva-treated bovine enamel slabs for determining the potential of chemotherapeutic agents to adsorb to tooth surfaces and act against plaque-forming bacteria. chlorhexidine was found to inhibit the formation of in vitro plaque by actinomyces viscosus, a naeslundii, streptococcus mutans and s sanguis. actinobolin was found to have marked antibacterial properties but limited adsorptive qualities.1977268336
fate of heterospecific transforming dna bound to streptococcus sanguis.the fate of 3h-labeled str-r fus-s dna from streptococcus pneumoniae, bound after a 1-min uptake to 14c-labeled str-s fus-r s. sanguis recipients, was followed by techniques previously developed for analyzing the fate of homospecific dna. heterospecific s. pneumoniae dna was bound and formed complexes with recipient protein in a manner similar to that of homospecific dna but transformed relatively poorly. the rate at which complexed heterospecific dna becomes physically associated with recipient ...197825262
the microbiology and biochemistry of plaque, saliva, and drinking water from two communities with contrasting levels of caries in colombia, s.a. 197724649
effect of fissure sealing on the microflora in occlusal fissures of human teeth. 1977270316
activation of the complement system by some gram-positive oral bacteria. 1977270324
effect of ph and counter ions on the zeta-potential of oral streptococci. 197723746
electron microscopic study of the interaction of oral microorganisms with polymorphonuclear leukocytes. 1977272141
enamel microhardness and fluoride uptake underneath fermenting and non-fermenting artificial plaque.washed cells of streptococcus sanguis were used to form artificial plaque on the surface of bovine enamel and incubated underneath buffer solutions, initial ph 6, for 36 h at 37 degrees c. the decrease in the microhardness of the enamel surface under fermenting "plaque" could be prevented with fluoride. enamel under a fermenting "plaque" took up significantly more (p less than 0.0u) fluoride than enamel under a non-fermenting "plaque" (initial f- in buffer: 10 parts/10(6)). the artificial plaque ...197722924
secretion of lipids induced by inhibition of peptidoglycan synthesis in streptococci.inhibition of peptidoglycan synthesis causes an immediate and massive secretion of both newly synthesized and "old" lipids from several species of bacteria, including streptococci, staphylococcus epidermidis, and bacillus subtilis. lipid secretion occurs in the absence of detectable bacterial lysis. this novel phenomenon was examined in more detail in three strains of streptococci: s. sanguis (group h), s. pyogenes (group a), and s. pneumoniae. the secretion of lipids is specifically induced by ...197721168
[recurrent aphthous stomatitis in the light of immunologic studies]. 1978274257
microbiota of gingivitis in man.a study on the predominant cultivable microorganisms inhabiting gingival crevices affected with a chronic gingivitis was carried out using the roll tube culture technique. samples were obtained from nine individuals 25--42 years of age. gram-positive rods make up 29.1% of the isolates and included mainly actinomyces naeslundii, actinomyces israelii, and actinomyces viscosus. streptococcus mitis and streptococcus sanguis together made up 26.8% of the cultivable organisms. peptostreptococcus avera ...1978276916
characterization of group h streptococcal temperate bacteriophage phi 227.phi 227, a temperate phage from a group h streptococcus (streptococcus sanguis), was propagated vegetatively in group h strain wicky 4-eryr, and its characteristics were determined. a procedure dependent on multiplicity of infection, incubation time, and treatment of crude lysates with diatomaceous earth was found to optimize phage yield, resulting in titers of 1 x 10(10) to 2 x 10(10) pfu/ml. without prior treatment with diatomaceous earth, subsequent purification procedures (methanol, ammonium ...197715133
glucosyltransferase production by streptococcus sanguis 804 (nctc 10904).streptococcus sanguis 804 (nctc 10904) was grown ih batch culture at constant ph. and the glucosyltransferase activity of the supernatant was assayed over a 40-h growth period. the optimum ph for enzyme production was 7.0 to 7.2. during growth of the culture, three reproducible phases of enzyme activity were observed. the polysaccharides synthesized during each of these phases were characterized as dextran-like glucans by analysis of acid hydrolysates, gas-liquid chromatography, and a specific a ...197713040
autolysis in strains of viridans streptococci.seven strains of viridans streptococci of the species streptococcus sanguis, s. mutans and s. mitis were investigated for autolysis. the effect of ph, salt concentration and temperature on the autolytic process was studied in na2hpo4/nah2po4 buffer. whole cells and walls of all strains autolysed most rapidly at ph values above 7. autolysis of whole cells of s. sanguis and one strain of s. mitis (atcc15909) was maximal in 0-05 to 0-2 m buffer, while the two s. mutans strains and s. mitis atcc1591 ...197610349
distribution of neisseria, rothia and streptococci in early stages of dental plaque. 1978279416
the viability of microorganisms in carious lesions five years after covering with a fissure sealant.carious lesions that were covered with a pit and fissure sealant for five years yielded bacterial cultures that were predominantly negative. sixteen of 18 test sites judged to have active caries in 1972 were found inactive in 1977; ten of 12 sites suspected to have caries in 1972 were deemed to have inactive caries in 1977. sealant treatment resulted in an apparent 89% reversal from a caries-active to a caries-inactive state. these data confirm and extend previous observations that a limited num ...1978279600
dental caries in gnotobiotic rats immunized with purified glucosyltransferase from streptococcus sanguis. 1978280287
ph and eh in single and mixed culture bacterial plaque in an artificial mouth. 19765393
effect of ph on competence development and deoxyribonucleic acid uptake in streptococcus sanguis (wicky).streptococcus sanguis (wicky) cells, strain we4, developed little or no competence and failed to autolyze in permissive conditions when treated with competence factor (cf) below ph 7.0. this lack of activity was directly correlated with the inability of the cells to bind or take up cf at ph values of 5.5, 6.0, and 6.5. on the other hand, competent cells bound deoxyribonucleic acid molecules maximally below ph 7.0 and transformed maximally at ph 6.5. deoxyribonucleic acid was optimally bound to c ...19764422
genetic hybridization at the unlinked thy and str loci of streptococcus.the sanguis and pneumoniae species of streptococcus were used as recipients in transformations from str+ to str-r and from thy- to thy+. the str-r mutations in the two species had been previously shown to be allelic. homology of the thy- mutations in the two species was demonstrated in the similar phenotypic properties they conferred (death in the absence of thymidine, lack of thymidylate synthetase). the str and thy loci are unlinked in each species.--- when the two species are transformed by b ...19751325
transformation of streptococcus sanguis to a rough colonial morphology with an increased ability to adhere. 1978283743
salivary agglutinin and secretory iga reactions with oral streptococci.s. mutans, s. salivarius, s. mitis and s. sanguis strains were isolated from three subjects of blood groups a, b and o. parotid saliva samples obtained from the same subjects induced aggregation of some of the bacteria, the s. sanguis and the s. mutans strains in particular. while parotid saliva from the three subjects gave almost identical reactions, slight interindividual differences were observed for a few strains with the submandibular sublingual salivas. in parotid saliva, secretory antibod ...1978284567
bacterial endocarditis due to penicillin-resistant streptococcus viridans.bacterial endocarditis remains a formidable diagnostic and therapeutic problem for clinicians. streptococcus viridans still accounts for 45 to 50 per cent of all cases and between 5 to 10 per cent of all clinical isolates of streptococcus viridans from patients with bacterial endocarditis may be relatively resistant to penicillin. the case of a 9-year-old child with tetralogy of fallot and a waterston shunt who subsequently developed bacterial endocarditis due to penicillin-resistant streptococc ...1979109241
primary structure of a human iga1 immunoglobulin. iv. streptococcal iga1 protease, digestion, fab and fc fragments, and the complete amino acid sequence of the alpha 1 heavy chain.in order to establish the complete amino acid sequence of the human iga alpha1 chain bur, iga1 protease from streptococcus sanguis was employed to generate fabalpha and fcalpha fragments in the final stage of this investigation. cyanogen bromide cleavage of the fabalpha fragment followed by reduction and aminoethylation produced the fd' fragment (residues 84 to 227); this contains part of the variable region (vr), the whole first constant domain (calpha1), and part of the hinge region of this he ...1979107164
[biological activity of secretory iga--particularly agglutinating activity of salivary siga against oral indigenous microorganisms (author's transl)]. 1978285194
studies on extracellular proteases of streptococcus sanguis. purification and characterization of a human iga1 specific protease.extracellular caseinolytic activity was found in the culture fluid of streptococcus sanguis atcc 10556 grown in a dialyzed culture medium. this activity was due to multiple proteases that differed in their elution from hydroxyapatite, sensitivity to enzyme inhibitors, specificity and optimum ph. iga protease, which splits human immunoglobulin a1 into intact fc and fab could be effectively separated from these relatively non-specific proteases and purified to apparent homogeneity in 20% yield by ...1978102357
bacterial endocarditis: a clinical report. 1979286207
characterization of a small plasmid determining resistance to erythromycin, lincomycin, and vernamycin balpha in a strain of streptococcus sanguis isolated from dental plaque.a plasmid determining resistance to erythromycin (inducible), lincomycin, and vernamycin b(alpha) was isolated from a strain of streptococcus sanguis isolated from dental plaque of a patient who had been on erythromycin therapy for an extended period of time. the plasmid, designated pam77, had a molecular weight of 4.5 x 10(6) and was present to the extent of one to two copies per bacterial chromosome. the plasmid could transform s. sanguis strain challis.197896738
acid production from alditols by oral streptococci. 1979286721
experimental infections with staphylococcus aureus, streptococcus sanguis, pseudomonas aeruginosa, and bacteroides fragilis in the jaws of dogs. 1979114906
factors influencing the deposition and growth of oral organisms on hard surfaces. 1979287687
reduction in proportions of dental plaque streptococci following a 5 day topical kanamycin treatment. 1977137306
interbacterial aggregation of actinomyces naeslundii and dental plaque streptococci. 1977137307
strain differences in binding of parotid saliva basic glycoprotein by oral streptococci. 1979291626
differential utilization of proteins in saliva from caries-active and caries-free subjects as growth substrates by plaque-forming streptococci.mixed or parotid saliva from caries-active individuals consistently supported better growth of streptococcus mutans (type c) than that from caries-free individuals. electrophoretic studies revealed that certain proteins in caries-active salivas were susceptible to microbial attack, but similar proteins in caries-free salivas were refractory.1979291630
canker sores and cold sores their diagnosis and treatment. 1976180593
structure of extracellular water-soluble polysaccharides synthesized from sucrose by oral strains of streptococcus mutans, streptococcus salivarius, streptococcus sanguis and actinomyces viscosus. 1979292363
comparative study of two variants of the mouth streptococcus sanguis with different colonial morphologies and abilities to adhere. 1979295202
the epidemiology of dental caries and periodontal disease.dental caries and periodontal disease are common in industrialized countries and caries is increasing in non-industrialized countries. the predominant method of coping with the disease problem has been to treat rather than prevent the diseases, an approach which has obviously failed to cope with the problem as shown by the results of the international collaborative study of dental manpower systems (58). epidemiological evidence suggests that a food policy directed at lowering the refined sugar c ...1979295300
co-induction of beta-galactosidase and the lactose-p-enolpyruvate phosphotransferase system in streptococcus salivarius and streptococcus mutans.the addition of lactose, galactose, or isopropyl-beta-d-thiogalactoside (iptg) to glucose-grown cells of streptococcus salivarius 25975 resulted in the co-induction of both the lactose-p-enolpyruvate phosphotransferase system (lactose-pts) and beta-galactosidase, with the latter the predominant metabolic system. with various strains of streptococcus mutans and streptococcus sanguis 10556, on the other hand, the lactose-pts was the major metabolic pathway with beta-galactosidase induced either to ...1978214423
glucose-6-phosphate-dependent pyruvate kinase in streptococcus mutans.pyruvate kinase of streptococcus mutans jc 2 had an absolute and specific requirement for glucose-6-phosphate. inorganic phosphate was a strong inhibitor. the enzyme required k+ or nh4+ and mg2+ or mn2+. s. mutans fil and e 49, streptococcus bovis atcc 9809, and streptococcus salivarius atcc 13419 had also glucose-6-phosphate-dependent pyruvate kinases, whereas streptococcus sanguis nctc 10904 had an enzyme activated by fructose-1,6-diphosphate.1975240810
surface ultrastructure of some oral bacteria.adhesion of streptococcus sanguis, fusobacterium nucleatum and an actinomyces sp. to enamel and epon and their interspecies cohesion was studied with scanning and transmission electron microscopy. for adhesion studies enamel or epon was coated with salivary macromolecules and then cells of s. sanguis and in some experiments also with f. nucleatum or actinomyces sp. cells of s. sanguis were seen scattered over the surface of a thin "pellicle" that was heavily stained, and f. nucleatum and actinom ...1979296564
recovery and clearance rates of oral microorganisms following experimental bacteraemias in dogs. 1975242303
serum antibodies to plaque bacteria in subjects with dental caries and gingivitis.correlations were sought between indices of gingival inflammation and dental caries experience and serum antibody titers to five species of oral bacteria. the material comprised 53 young adult males. a statistically significant, negative correlation was observed between the antibody titer to a pool of veillonella strains and dental caries experience. multiple regression analyses failed to reveal significant associations between periodontal disease and serum antibody titers. however, the data sug ...1977320647
antagonistic substances produced by streptococci from human dental plaque and their significance in plaque ecology. 1977267511
survival of bacteria from human dental plaque under various transport conditions.the effects of transport media, temperature, and anaerobiosis on the survival of bacteria from human supragingival dental plaque were studied. individual samples were obtained by passing sterile, unwaxed dental floss through the interproximal spaces. the plaque-bearing portion of floss was immediately placed in vials containing reduced transport fluid, viability-preserving microbistatic medium, or reduced salt solution transport fluid. plaque samples were dispersed by ultrasonic oscillation, ser ...1977332708
[problems of the relationship between oral hygiene and dental caries]. 1977267561
inhibition of glucose utilization in oral streptococci by low concentrations of fluoride. 1977269750
[streptococcal flora of normal and inflamed gingival pockets]. 1977269783
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