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changes in the microflora and physiology of the anterior intestinal tract of pigs weaned at 2 days, with special reference to the pathogenesis of diarrhea.the gastrointestinal microflora and gastric physiology of piglets weaned at 2 days was compared with that of piglets allowed to continue sucking the sow. although there was a significantly higher count of escherichia coli in the stomach, duodenum, and jejunum of the early-weaned compared with sow-reared pigs, these differences were not detectable in samples from the ileum. there were no quantitative differences in lactobacilli and in streptococci between the two treatments. lactobacillus ferment ...197722488
factors affecting the aggregation of actinomyces naeslundii during growth and in washed cell suspensions.various factors affecting the aggregation of actinomyces naeslundii strain 12104 were studied. when the ph of glucose-supplemented growth medium fell below 5.5, the cells aggregated and formed microbial masses which tenaciously adhered to the culture vessels. when the organism was cultured in the same medium in the absence of glucose, maximum growth was reduced and the final culture ph values remained above 6.5, but the cells were more dispersed and nonadherent. adjusting the final ph of these c ...197830700
extractability of cell wall polysaccharide from lactobacilli and streptococci by autoclaving and by dilue acid.autoclaving cell wall of streptococcus mutans ingbritt for 15 min under the rantz and randall conditions released one-tenth of the total cell wall carbohydrate, whereas two-thirds was extracted after autoclaving for 180 min. the extract contained the serotype c-specific antigen but lacked the lipoteichoic acid component extracted when whole cells were autoclaved. autoclaving cell wall preparations from other strains of s. mutans and also streptococcus salivarius and streptococcus mitis in 0.85% ...197832140
fine structure and distribution of extracellular polymer surrounding selected aerobic bacteria.the structure and distribution of extracellular polymer surrounding bacillus circulans, diplococcus (streptococcus) pneumoniae, streptococcus salivarius, staphylococcus aureus, klebsiella pneumoniae, pseudomonas aeruginosa, herella vaginacola (acinetobacter calcoaceticus), and agrobacterium tumefaciens were studied by electron microscopy. a modified ruthenium red staining procedure was used to examine the fine structure of capsule and slime. freeze-etching and critical-point drying were used to ...197546774
genital occurrence of oral microbiota.recent studies indicate that tonsillar gonococcal infection or colonization is fairly common. carriage rates of about 8% have been found. these studies also indicate that oro-genital contacts are common. since very little is known about the amount of oral microbiota transmitted to the genitals, we have studied the occurrence of oral streptococci and neisseria species in urethra and cervix. among 128 patients attending an std-clinic we found 10 carriers of oral streptococci, one streptococcus mit ...197882332
interactions between human serum proteins and oral streptococci reveal occurrence of receptors for aggregated beta 2-microglobulin.a total of 31 strains of oral streptococci representing streptococcus mutans, streptococcus sanguis, streptococcus mitior, streptococcus salivarius, and streptococcus milleri were tested for possible binding of human immunoglobulins g, g1, g2, g3, g4, a1, a2, m1, and m2 and haptoglobin, hemoglobin, fibrinogen, and aggregated beta 2-microglobulin. radiolabeled beta 2-microglobulin in aggregated form showed affinity for 20 of the 31 strains tested. binding activity for the protein was found in str ...197990015
streptococcus sanguis and streptococcus salivarius in early plaque formation on plastic films. 1977143517
co-induction of beta-galactosidase and the lactose-p-enolpyruvate phosphotransferase system in streptococcus salivarius and streptococcus mutans.the addition of lactose, galactose, or isopropyl-beta-d-thiogalactoside (iptg) to glucose-grown cells of streptococcus salivarius 25975 resulted in the co-induction of both the lactose-p-enolpyruvate phosphotransferase system (lactose-pts) and beta-galactosidase, with the latter the predominant metabolic system. with various strains of streptococcus mutans and streptococcus sanguis 10556, on the other hand, the lactose-pts was the major metabolic pathway with beta-galactosidase induced either to ...1978214423
effect of ph on the biosynthesis of levan and on the growth of streptococcus salivarius. 1975240341
glucose-6-phosphate-dependent pyruvate kinase in streptococcus mutans.pyruvate kinase of streptococcus mutans jc 2 had an absolute and specific requirement for glucose-6-phosphate. inorganic phosphate was a strong inhibitor. the enzyme required k+ or nh4+ and mg2+ or mn2+. s. mutans fil and e 49, streptococcus bovis atcc 9809, and streptococcus salivarius atcc 13419 had also glucose-6-phosphate-dependent pyruvate kinases, whereas streptococcus sanguis nctc 10904 had an enzyme activated by fructose-1,6-diphosphate.1975240810
[synthesis of extracellular polysaccharides by various forms of streptococcus salivarius].streptococcus salivarius synthesizes extracellular polysaccharides (eps) from saccharose under aerobic and anaerobic conditions. under aerobic conditions primarily soluble fructanes are formed. there are no differences between the eps synthesized by r- and s-forms under aerobic conditions; anaerobically the s-forms produce more eps and more fructane than the r-forms. as compared to streptococcus mutans, the streptococcus salivarius produces considerably more fructane and insoluble glucane under ...1977269074
amino acid requirements and human saliva as a nitrogen source for streptococcus salivarius and streptococcus mitior. 1978277499
the effect of some divalent cations on extracellular polysaccharide synthesis in streptococcus salivarius.the amount of extracellular insoluble polysaccharide produced by streptococcus salivarius can be effected by some divalent cations. calcium at concentrations of 1 x 10(-3) and 10(-4) m caused a reduction in polysaccharide synthesis. magnesium at 1 x 10(-3) m inhibited extracellular polysaccharide production but at 1 x 10(-4) m had little effect. manganese was without effect on polysaccharide synthesis. zinc at 1 x 10(-3) and 1 x 10(-4) m caused a substantial increase in extracellular polysacchar ...1978277538
structure of extracellular water-soluble polysaccharides synthesized from sucrose by oral strains of streptococcus mutans, streptococcus salivarius, streptococcus sanguis and actinomyces viscosus. 1979292363
antigenic variation in populations of streptococcus salivarius isolated from the human mouth. 1979293150
improved immunoadsorption procedure with anion-exchange bacterial cell columns.bacterial cell columns for immunoadsorption were prepared with streptococcus cells and triethylaminoethyl cellulose (cellex-t) matrix material as a model system. good column flow properties and satisfactory retention of the cells were obtained with ratios as high as 2 ml of packed cells/3 g dry weight of cellulose. anion-exchange fractionation of whole serum by the cellex-t was prevented by using 0.25 m nacl in the developing buffer. antibodies were adsorbed directly from whole serum and recover ...1978351066
circulating immune complexes in experimental streptococcal endocarditis: a monitor of therapeutic efficacy.an important problem in the management of infective endocarditis has been the delineation of laboratory procedures that are sensitive, reliable indicators of therapeutic efficacy. because circulating, complement-containing immune complexes of the igg type (cics) have been demonstrated in most humans with infective endocarditis, serum cic levels during the natural course of the infection and in response to penicillin therapy were studied in 42 rabbits with right-sided endocarditis due to streptoc ...1979438528
structure of the water-insoluble alpha-d-glucan of streptococcus salivarius hht.water-insoluble, non-adherent alpha-d-glucans have been obtained from streptococcus salivarius hht under two sets of conditions: from a growing culture, or synthesized enzymically by using a glucosyltransferase. in the former case, the glucan ([alpha]d + 197 degrees) was shown by methylation analysis to have a slightly branched structure containing a relatively high proportion (80%) of (1 leads to 3)-alpha-d-glucosidic linkages, together with small proportions of (1 leads to 6)- and (1 leads to ...1979487362
[femoral osteitis with in situ isolation of streptococcus salivarius]. 1979548920
adherence of veillonella species mediated by extracellular glucosyltransferase from streptococcus salivarius.the effect of extracellular products from streptococcus salivarius on sucrose-dependent adherence to smooth surfaces by other oral bacteria was studied in vitro. strains of streptococcus mitis, streptococcus pyogenes, and veillonella parvula without innate ability to adhere to a steel wire were able to do so when incubated with sucrose and cell-free culture fluid from s. salivarius strains 9759, 25975, cnii, and mepi. these culture fluids synthesized more adherent material and water-insoluble gl ...1977591064
tween 80 effect on glucosyltransferase synthesis by streptococcus salivarius.streptococcus salivarius (atcc 25975) produced very low or nondetectable amounts of the extracellular enzyme glucosyltransferase (gtase) when grown in a chemically defined medium. the addition of tween 80 to this medium resulted in the production of markedly enhanced levels of the enzyme. oleic acid, the methyl ester of oleic acid, and sucrose each could not substitute for tween 80 in this regard. the surfactant had no direct activating effect on performed enzyme activity. tween 80 also stimulat ...1978618839
streptococcus salivarius sepsis in children with malignancies. 1978621618
bacteria associated with the gastric epithelium of neonatal pigs.light and electron microscopy showed lactobacilli and, to a lesser degree, streptococci to be closely associated with the squamous area of the pig stomach known as the pars esophagea. several different types of extracellular layers were seen on bacteria attached to the epithelial surface. the total number of bacteria per square centimeter did not change with age up to 10 days, and there was no effect of weaning at 2 days. lactobacillus fermentum, l. salivarius, and streptococcus salivarius were ...1978637551
a study of the interaction between oral streptococci and hard surfaces.a rotating disc method was used to compare the tendencies of two oral streptococci to deposit on to glass and polystyrene surfaces from electrolyte solutions of varying ionic strength. streptococcus salivarius had a greater tendency to deposit than had streptococcus mitior under these condition. in addition to the balance of van der waals' forces of attraction and electrostatic forces of repulsion, it is suggested that the adsorption to the glass and polystyrene surfaces of material present in t ...1978641524
inhibitory effects of extracellular products from oral bacteria on human fibroblasts and stimulated lymphocytes.extracellular products of 12 strains of streptococcus mutans and 5 additional species of oral bacteria were analyzed for their ability to inhibit proliferation of fibroblastoid cells (hela and av3) and blast transformation of human peripheral blood lymphocytes obtained from normal individuals. products from s. mutans strains aht and bht, streptococcus intermedius, and actinomyces viscosus inhibited [3h]thymidine uptake by fibroblastoid cells and phytohemagglutinin-stimulated lymphocytes. product ...1978689736
determinants of the developing oral flora in normal newborns.the ability of streptococcus species to selectively adhere to the oral epithelial cells of newborns was studied in vitro. on day 1 of life, mucosal cells from normal infants demonstrated selective attraction for the natural distribution of streptococci that would soon colonize these surfaces. streptococcus salivarius and streptococcus mitis adhered well in vitro to scraped cells from cheek and tongue surfaces. streptococcus mutans, on the other hand, exhibited feeble or no adherence to cheek or ...1976791120
changing agglutination activities of salivary immunoglobulin a preparations against oral streptococci.immunoglobulin a (iga) was prepared from samples of parotid saliva collected from two individuals over a 150-day period. standardized solutions of the iga were tested for agglutinating antibody activity against strains of streptococcus salivarius, s. sanguis, s. mitis, and s. mutans that had been isolated from a homologous individual on day 30 and preserved in the lyophilized state. the agglutinating activity of the standardized iga preparations against these strains was found to change over tim ...1975803930
selective adherence as a determinant of the host tropisms of certain indigenous and pathogenic bacteria.the relationship between the selective abilities of bacteria to adhere and their predilections for colonizing different mammalian hosts was investigated by using bacteria indigenous to the tongue dorsum of humans and rats as models. streptococcus salivarius and s. sanguis averaged 22.6 and 2.8%, respectively, of the cultivable bacteria recovered from swab samples of the tonges of five humans, but these organisms were not indigenous on the tongues of rats (charles river strain). s. faecalis and s ...1976814100
antagonistic action of streptococcus salivarius and streptococcus faecalis to mycobacterium tuberculosis.streptococcus salivarius and streptococcus faecalis were found to inhibit the growth of mycobacterium tuberculosis on löwenstein-jensen and middlebrook 7h11 agars, but not on the latter medium when antibacterial drugs were added. s. faecalis was found to be more inhibitory than s. salivarius to 15 strains of m. tuberculosis. s. salivarius produced little or no inhibition of growth of runyon group iii organisms but was very antagonistic to runyon group i mycobacteria.1976824304
structure of the levan elaborated by streptococcus salivarius strain 51: an application of chemical-ionisation mass-spectrometry.the polysaccharide elaborated by streptococcus salivarius strain 51 contains beta-d-fructofuranose residues linked through positions 2 and 6, as well as 1, 2, and 6. the approximate numbers of terminal, non-reducing d-fructofuranose residues and those linked through positions 2 and 6, and through 1, 2, and 6 in the average repeating-unit are 1, 7, and 1, respectively. the branches through the beta-(2 leads to 1)-linkage contain up to at least four d-fructofuranose residues. chemical-ionisation m ...1976963696
levans: i. fractionation, solution viscosity, and chemical analysis of levan produced by streptococcus salivarius.levan produced by streptococcus salivarius was fractionated into a series of 20 fractions of varying molecular weight. the range of intrinsic viscosities of the fractions was 0.07 to 0.18 dl/gm in water and 0.20 to 0.29 dl/gm in dimethyl sulfoxide. the molecular weight of the unfractionated leval determined by light scattering was 31.5 times 10-6. small amounts of fatty acids and protein were found associated with levan.19751054339
streptococcus salivarius, streptococcus sanguis and streptococcus "mitis" as indicators of contamination of root canal cultures.small samples were taken from the first drop of saliva entering an operation field for root canal treatment isolated by rubber dam, which was intentionally leaky. the samples were primarly cultured in tas- and blh-medium and on ms-agar aerobically. the culture media were inoculated directly and via transport medium (vmg iii). the cultures were analysed for strep. salivarius, strep. sanguis and/or strep. "mitis" after subculture on ms-agar as soon as visible growth had appeared in the broth media ...19751057108
purification and properties of a cell-associated levan-sucrase from streptococcus salivarius ss2. 19761068648
growth of several cariogenic strains of oral streptococci in a chemically defined medium.a chemically defined medium in which streptococcus mutans strains aht, bht, gs-5, jc-2, ingbritt, at6t, at9t, 6715, and omz-176 and streptococcus salivarius strain hht grew rapidly to high turbidities was formulated. maximal turbidities of each strain were observed after 8 to 12 h of aerobic growth. the subsequent transfer of exponentially growing cells into fresh medium resulted in growth at the same rate without lag. growth of these strains occurred with rates at least one-half of those observ ...19751091546
amino acid requirements of streptococcus mutans and other oral streptococci.the amino acid requirements of streptococcus mutans strains aht, omz-61, fa-1, bht, gs-5, jc-2, ingbritt, at6t, omz-176, 6715, streptococcus salivarius hht, streptococcus sanguis omz-9, and strain 72x46 were determined in a chemically defined medium. when grown anaerobically in the presence of sodium carbonate (or bicarbonate for a few strains), few amino acids were required. all strains tested required cystine (or cystine) as a nutrient. three strains (s. mutans omz-176, fa-1, and bht) required ...19751091547
purification and characterization of two bacteriocins from streptococcus faecium.two bacteriocins were found in the supernatant fluid and in an extract of streptococcus faecium strain ei. the small soluble enterocin eia represented more than 90% of the total activity in the supernatant fluid, and was purified 400-fold by ammonium sulphate fractionation, gel filtration on sephadex g-75 and chromatography on deae-cellulose. enterocin eib, with a particle weight greater than 4 x 10(6), was the predominant type in the extract. it was released in appreciable quantities after brea ...19751097585
[studies on the relationship of temperent phages and bacteriocines of streptococcus faecium (author's transl)].two bacteriocins (enterocin e1a and e1b) as well as a complete bacteriophage (pe1) were produced by streptococcus faecium strain e1. although the phage could be demonstrated by electron microscopy it was not possible to observe phages or phage-like particles in the purified preparation of the large enterocin e1b. phage pe1 had a much smaller activity spectrum than that of enterocin e1a and e1b, inhibiting only one strain of streptococcus faecium and one strain of streptococcus salivarius. the en ...19751101576
bacterial interference by streptococcus salivarius.a strain of streptococcus salivarius, recognized in a throat culture because of its inhibition of the growth of a species of corynebacterium, was studied in vitro to determine its antagonistic effects agains various other bacteria. it was found to be inhibitory to anaerobic gram-positive cocci, streptococci belonging to lancefields groups a, c, f, and g, and corynebacterium diphtheriae, c. hofmanii, and c. xerosis.19751155368
levans. ii. light-scattering and sedimentation data of streptococcus salivarius levan in water. 19751164548
evaluation of human oral organisms and pathogenic streptococcus for production of iga protease.iga protease is a proteolytic enzyme found in whole human saliva and in dental plaque that cleaves both secretory and myeloma iga of human origin to yield intact fabalpha and fcalpha fragments. to determine which bacteria are capable of producing this enzyme, we have examined a variety of strains normally found in the human oral cavity and a number of streptococci of known lancefield group serotype. streptococci of groups a, b, c, d, f, g, h, m, and n, streptococcus mutans, streptococcus sanguis ...19751168676
immunoadjuvant activities of cell walls and their water-soluble fractions prepared from various gram-positive bacteria.the cell walls from all 21 species of gram-positive bacteria examined, except lysozyme-susceptible micrococcus lysodeikticus (nctc 2665) and lysozyme-resistant staphylococcus epidermidis (atcc 155), were found to be definitely adjuvant-active in both stimulation of increased serum antibody levels and induction of delayed-type hypersensitivity to ovalbumin when administered to guinea-pigs as water-in-oil emulsions. using various cell wall lytic enzymes, the immunoadjuvant principles were solubili ...19751180872
depth profiling of the elemental surface composition of the oral microorganism s. salivarius hb and fibrillar mutants by x-ray photoelectron spectroscopy.x-ray photoelectron spectroscopy (xps) on microbial cell surfaces requires freeze-drying of cells, and as a result, the cell surface appendages flatten out on the cell surface and form a collapsed fibrillar mass. at present, it is unclear how the density, length and composition of these fibrils influence the elemental surface composition as probed by xps. the sampling depth of xps can be varied by changing the electron take-off angle. in this article, we made a depth profiling of the collapsed f ...19921284785
radiofrequency gas plasma (glow discharge) disinfection of dental operative instruments, including handpieces.the radiofrequency-stimulated argon gas plasma (glow discharge) technique, already well-known for surface cleaning and activation of adhesion, was investigated for determination of its separate potential for rapid disinfection of dental operative instruments. disinfection effectiveness was judged from diminished post-treatment recovery of viable organisms from the instruments agitated in saline. streptococcus salivarius, bacillus stearothermophilus, and escherichia coli were used as primary cont ...19921289559
immunoglobulin a antibody activity to mutans streptococci in parotid, submandibular and whole saliva.antigen extracts of 9 strains of streptococcus mutans, streptococcus sobrinus, streptococcus salivarius and streptococcus mitis were separated in acrylamide gels by electrophoresis. strains of the same genotype of mutans streptococci were found to have virtually identical sodium dodecyl sulfate-polyacrylamide gel electrophoresis patterns but differed clearly from other genotypes of mutans or with the reference strains s. salivarius and s. mitis. immunoglobulin a (iga) antibody activity to antige ...19921299799
lithium chloride-sodium propionate agar for the enumeration of bifidobacteria in fermented dairy products.lithium chloride-sodium propionate agar has been developed for the enumeration of bifidobacteria in fermented dairy products. the medium contains lithium chloride and sodium propionate to inhibit the growth of other lactic acid bacteria. pure cultures of bifidobacteria, lactobacilli, and streptococci were tested for growth in this medium. with one exception, all bifidobacteria were able to grow in this medium and in a nonselective agar with a difference not exceeding .4 log units. however, none ...19921317893
different bacteriophage resistance mechanisms in streptococcus salivarius subsp. thermophilus.streptococcus salivarius subsp. thermophilus strain nst5 exhibited a temperature-dependent defence mechanism against the virulent bacteriophages phi b1.2 and phi a1.1. it was active at 42 degrees c but not at 30 degrees c as demonstrated by a significant increase of both plaque size and efficiency of plaquing. this defence mechanism did not affect host-dependent phage replication and did not interfere with phage adsorption to nst5. these results suggest that it interfered with phage development. ...19921328335
the 23s-5s spacer of two rrna loci of streptococcus salivarius subsp thermophilus includes a promoter.the nucleotide sequence of the 3' part of a ribosomal and transfer rna locus from streptococcus salivarius subsp thermophilus nst1403 was determined. the sequenced dna fragment includes the 3' end of a 23s rrna gene, a 5s rrna gene, a trna(asn) gene and a potential transcriptional terminator. the trna gene does not encode for the cca 3'terminus of mature trna. we compared this sequence to a promoter-carrying dna fragment sequence (p20) of streptococcus salivarius subsp thermophilus a054 [1]. we ...19921381619
characterization of the streptococcus mutans gs-5 frua gene encoding exo-beta-d-fructosidase.the complete nucleotide sequence (5,010 bp) of the fructanase gene (frua) and flanking regions of the chromosome of streptococcus mutans gs-5 was determined. the frua gene appears to be the sole transcript arising from a proximal promoter. the presumed precursor of the secreted frua protein consists of 1,423 amino acids, and it has an m(r) of 158,656 and a pi of 4.82. the n terminus of frua has characteristics in common with signal peptides of gram-positive organisms. the c terminus consists of ...19921398976
growth interaction between candida albicans and streptococcus salivarius: in vitro and in vivo studies.suppression of candida albicans in the mouth by oral flora has been proposed as one of the mechanisms preventing candidal overgrowth. according to liljemark and gibbons (1973), streptococcus salivarius plays a significant role in this process. the aim of this investigation was to study the growth interaction between c. albicans and s. salivarius in vitro and in vivo. an aerobic continuous-flow system was used for the in vitro study. pure and mixed cultures of c. albicans (ncpf 3118) and s. saliv ...19921401427
processes involved in the regulation of urease levels in streptococcus salivarius by ph.urease levels in streptococcus salivarius are regulated by ph. there is either increased synthesis at low ph (5.5) or enhanced degradation at neutral ph, suggested by urease instability during stationary phase. to establish which mechanism predominates, protein synthesis was inhibited by chloramphenicol (cap) in cultures grown at ph 5.8, 6.8 and 7.3. there was no significant urease degradation detectable in mid-exponential and early-stationary phase at any ph. urease degradation occurred in both ...19921408352
cloning, sequencing and expression in escherichia coli of the ptsi gene encoding enzyme i of the phosphoenolpyruvate:sugar phosphotransferase transport system from streptococcus salivarius.we present the cloning and sequencing of the ptsi gene, encoding enzyme i (ei) of the phosphoenolpyruvate (pep): sugar phosphotransferase (pts) transport system from streptococcus salivarius. the ptsi gene corresponds to an open reading frame of 1731 nucleotides, which translates into a putative 577-amino acid (aa) protein with a m(r) of 62,948 and a pi of 4.49. the ei was produced in escherichia coli under the control of its own promoter located immediately upstream of ptsi, a situation never p ...19921427100
physico-chemical and structural properties of the surfaces of peptostreptococcus micros and streptococcus mitis as compared to those of mutans streptococci, streptococcus sanguis and streptococcus salivarius.the surface properties of nine streptococcus mitis and four peptostreptococcus micros strains from the oral cavity were examined and compared with a large group of oral streptococci. zeta potential and contact angle measurements were employed to determine physico-chemical cell surface properties. in addition, elemental surface concentration ratios were obtained via x-ray photoelectron spectroscopy, and surface structures were examined with transmission electron microscopy. the s. mitis and p. mi ...19921487734
streptococcus salivarius meningitis following myelography. 19921517542
expression of an inducible enzyme ii fructose and activation of a cryptic enzyme ii glucose in glucose-grown cells of spontaneous mutants of streptococcus salivarius lacking the low-molecular-mass form of iiiman, a component of the phosphoenolpyruvate:mannose phosphotransferase system.we have reported previously that the phosphoenolpyruvate:mannose phosphotransferase system (mannose pts) of streptococcus salivarius, consisting of an enzyme ii mannose (eiiman) and two forms of enzyme iii mannose (iiiman) with mr values of 38,900 and 35,200, respectively, concomitantly transports and phosphorylates mannose, as well as glucose and fructose. in this paper, we report the presence, in s. salivarius, of alternative specific fructose and glucose ptss encoded by inducible and cryptic ...19921534118
detection of propionic acid bacteria in cheese.mesophilic lactic starters and thermophilic lactobacilli but not streptococcus salivarius subsp. thermophilus grew on the sodium lactate agar (sla) used for estimating the numbers of propionic acid bacteria (pab) in cheese. the addition of cloxacillin (4 micrograms/ml) to sla inhibited the starter bacteria but had no effect on the pab. it was possible to count low numbers of pab in the presence of high numbers of starter bacteria. a correlation coefficient of 0.9 was obtained between the level o ...19921560128
evidence and characterization of temperate bacteriophage in streptococcus salivarius subsp. thermophilus st18.lysogenic strains of streptococcus salivarius subsp. thermophilus were studied using induction with mitomycin c (mc). the induction and presence of temperate phage were investigated carrying out tests on sensitive strains, electron microscopy and phage dna analysis. forty-five str. salivarius subsp. thermophilus strains were subjected to induction with mc and growth of the various cultures was evaluated. only one strain of those tested showed lysis after adding mc, thus indicating a possible lys ...19921560129
purification and partial characterization of an intracellular aminopeptidase from streptococcus salivarius subsp. thermophilus strain aca-dc 114.an intracellular aminopeptidase from streptococcus salivarius subsp. thermophilus strain aca-dc 114, isolated from traditional greek yoghurt, was purified by chromatography on deae-cellulose and sephadex g-100. the enzyme had a molecular weight of 89,000. it was active over a ph range 4.5-9.5 and had optimum activity on l-lysyl-4-nitroanilide at ph 6.5 and 35 degrees c with km = 1.80 mmol/l; above 55 degrees c the enzyme activity declined rapidly. the aminopeptidase was capable of degrading subs ...19921568949
retention of streptococci to defined solid surfaces in the presence of saliva secretions.the initial surface retention of streptococcus sanguis (g9b and atcc 10556) and streptococcus salivarius (atcc 9758 and atcc 13419) was examined using a well defined flow cell system. the microorganisms, known to be recovered from hard vs. soft tissue surfaces, respectively, were suspended in either ringer solution, human parotid saliva (hps), human submandibular and sublingual saliva (hsmsl), or mixed saliva. microbial retention was evaluated on germanium prisms of low (20-25 mnm-1) and medium ...19921574683
streptococcus salivarius bacteremia and meningitis following upper gastrointestinal endoscopy and cauterization for gastric bleeding.streptococcus salivarius is a normal inhabitant of the oral cavity. when isolated from blood cultures, this viridans streptococcus is often disregarded as a contaminant. viridans streptococci, although a common cause of endocarditis, are rarely associated with bacterial meningitis and account for less than 1% of all cases of purulent meningitis. we report a case of bacteremia and meningitis due to s. salivarius that occurred in a patient who underwent upper gastrointestinal endoscopy and cauteri ...19921576292
multibacterial artificial plaque. a model for studying carious process.a multibacterial artificial plaque was established in vitro on enamel slabs incubated in mixed culture of streptococcus sanguis, streptococcus mutans (serotype c), streptococcus salivarius, lactobacillus casei and actinomyces viscosus. typical structure same as natural plaque was seen under microscope. the caries-like lesion produced under the plaque was observed in enamel sections. the lesion was similar in appearance to natural ones under optic and scanning electron microscopes. the acids prod ...19921576866
streptococcal and enterococcal bacteremia in patients with cancer.eighty-two episodes of bacteremia due to streptococci (including the genus enterococcus) in 78 patients hospitalized at institut jules bordet between 1986 and 1988 were reviewed. the incidence ranged from 5.5 to 7.6 per 1,000 admissions (16% of all bacteremias). enterococcus faecalis, streptococcus sanguis, and streptococcus mitis were the most prevalent isolates, followed by streptococcus angionosus, streptococcus salivarius, and large colony-forming beta-hemolytic species (a, b, c, and g). twe ...19921617072
a general method for cloning reca genes of gram-positive bacteria by polymerase chain reaction.an internal fragment of the reca gene from eight gram-positive organisms has been amplified by using degenerate primers in a polymerase chain reaction. the internal 348- or 360-bp reca dna segments from bacillus subtilis, clostridium acetobutylicum, lactobacillus bulgaricus, lactobacillus helveticus, leuconostoc mesanteroides, listeria monocytogenes, staphylococcus aureus, and streptococcus salivarus subsp. thermophilus were amplified, cloned, and sequenced. the g + c contents of the dna from th ...19921629178
detection of intraspecific dna polymorphism in streptococcus salivarius subsp. thermophilus by a homologous rdna probe.three ribosomal probes from streptococcus salivarius subsp. thermophilus were cloned. sequence data demonstrate that their juxtaposition corresponds to an entire operon. they were used in order to study ribosomal operon number and organization. rrna genes were shown to be clustered in the order 5'-16s-23s-5s-3' and the number of rrn loci to vary within the subspecies. the smallest of the 3 probes was used for strain characterization. substantial variability in hybridization patterns was observed ...19921641511
a species-specific dna probe obtained from streptococcus salivarius subsp. thermophilus detects strain restriction polymorphism.genomic polymorphism in streptococcus salivarius subsp. thermophilus was revealed by dna restriction pattern analysis. a 4.2-kb variable dna fragment was cloned from strain nst7 and hybridised with the dna of 25 strains allowing an easy detection of intraspecific rflp. strong and weak hybridisation signals were observed and the latter were specifically revealed by a 2.1-kb fragment of the probe. probe specificity was demonstrated by the absence of homology with dna of strains belonging to 10 oth ...19911679405
purification and characterization of dipeptidyl peptidase iv from streptococcus salivarius hht.dipeptidyl peptidase iv (dap iv) was purified from streptococcus salivarius hht by anion-exchange chromatography, gel filtration and affinity chromatography after lysis of cell walls with n-acetylmuramidase. dap iv was purified 114-fold with a yield of 16.6% from total activity of the crude extract. the purified enzyme was shown to be homogeneous by disc gel electrophoresis. the molecular weight of the enzyme was estimated to be about 109,000 by gel filtration and 47,000 by sodium dodecylsulphat ...19911684633
oral ofloxacin therapy for lower respiratory tract infection.we made an open, noncomparative evaluation of ofloxacin, 400 mg orally bid for 10 days, in 98 subjects with community-acquired pneumonia or pathogen-confirmed bronchitis. thirty-nine (40%) of the subjects were treated in the hospital and 59 (60%) were treated as outpatients. the mean age of those treated was 56.2 years; 73 (74%) of the subjects either were more than 60 years old or had a history of chronic obstructive pulmonary disease, or both. there were 95 organisms initially isolated in sput ...19921734527
[maternal morbidity after abdominal cesarean section in relation to bacterial amniotic fluid colonization and premature rupture of fetal membranes].at the time of caesarean section, amniotic fluid was collected transabdominally from 1025 patients. in 990 patients cultures were performed on the amniotic fluid. the incidence of caesarean section of all patients was 16.1%, the perinatal mortality in all patients 0.61%, in cases of caesarean section 1.93%. the influence of bacterial colonisation in the amniotic cavity was analyzed with respect to premature ruptured membranes and frequency of vaginal examinations on the maternal post-operative m ...19911743472
the presence of two forms of the phosphocarrier protein hpr of the phosphoenolpyruvate:sugar phosphotransferase system in streptococci.the protein, hpr, a necessary component of the phosphoenolpyruvate phosphotransferase system (pts) in bacteria, was purified from streptococcus salivarius by column chromatography. the purified preparation gave only one band when analyzed by sodium dodecylsulfate gel electrophoresis or by isoelectric focusing in polyacrylamide gel (pi = 4.85). however, electrophoresis in tris-containing buffers under non-denaturing conditions revealed 2 bands that could be phosphorylated by pep in the presence o ...19911764502
purification and characterization of an endopeptidase from lactococcus lactis subsp. cremoris wg2.an endopeptidase has been purified to homogeneity from a crude cell extract of lactococcus lactis subsp. cremoris wg2 by a procedure that includes diethyl-aminoethane-sephacel chromatography, phenyl-sepharose chromatography, hydroxylapatite chromatography, and fast protein liquid chromatography over an anion-exchange column and a hydrophobic-interaction column. gel filtration and sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated a molecular mass of the purified enzyme of 70,000 ...19911785932
purification, properties, and sequence specificity of ssli, a new type ii restriction endonuclease from streptococcus salivarius subsp. thermophilus.ssli, a type ii restriction endonuclease, was purified from streptococcus salivarius subsp. thermophilus strain bsn 45. ssli is an isoschizomer of bstni. ssli activity was maximum at ph 8.8, 0 to 50 mm nacl, 2 to 8 mm mg2+, and 42 degrees c. activity against phage dna in vitro was demonstrated.19911785940
multilevel control of extracellular sucrose metabolism in streptococcus salivarius by sucrose.standardized experimental conditions were established to test the role of sucrose in the regulation of control of its metabolism in streptococcus salivarius. a fresh isolate of s. salivarius was used. the extracellular dextranase activity of cells grown on sucrose was 10-fold higher than that of cells grown on glucose, fructose or galactose. this activity increased in less than 5 min following the addition of sucrose to galactose-grown cells, a phenomenon which was affected by neither rifampicin ...19911828497
molecular characterization of a cluster of at least two glucosyltransferase genes in streptococcus salivarius atcc 25975.the oral micro-organism streptococcus salivarius atcc 25975 synthesizes extracellular glucosyltransferases (gtfs) which polymerize the glucose moiety of sucrose into glucan polymers. two separate genes encoding the activities of a gtf-i (a gtf that synthesizes an insoluble product) and a gtf-s (a gtf that synthesizes soluble product) were cloned into bacteriophage lambda l47.1. the inserts in the lambda-clones were characterized by restriction mapping and southern hybridization and were found to ...19911838391
partial characterization of the glucosyltransferases of an oral streptococcus salivarius strain.the oral streptococcus salivarius strain s3 possessed extracellular glucosyltransferase (gtf) and cell-associated fructosyltransferase activities. chromatofocusing column chromatography of crude gtf yielded multiple peak fractions, which were categorized into two types which catalysed (1) water-insoluble glucan synthesis and (2) water-soluble glucan formation. the gtf isozyme eluted in fraction 29 synthesized mutanase- and dextranase-sensitive insoluble glucan from sucrose, while fraction 81 gtf ...19911839049
isolation and characterization of chromosomal promoters of streptococcus salivarius subsp. thermophilus.a promoter probe vector, ptg244, was constructed with the aim of isolating transcription initiation signals from streptococcus thermophilus (streptococcus salivarius subsp. thermophilus). ptg244 is based on the escherichia coli-streptococcus shuttle vector ptg222, into which the promoterless chloramphenicol acetyltransferase gene of bacillus pumilus (cat-86) was cloned. random sau3a fragments from the s. thermophilus a054 chromosomal dna were cloned upstream of the cat-86 gene by using e. coli a ...19911854195
streptococcus salivarius meningitis and colonic carcinoma.streptococcus salivarius caused fulminant meningitis in an elderly patient. a gastrointestinal diagnostic workup revealed an asymptomatic colonic adenocarcinoma. this first reported instance of s salivarius sepsis associated with a colonic neoplasm is not unexpected, since the organism is bacteriologically similar to s bovis, the prime bacterial indicator of occult malignancy. exact speciation of streptococcal strains is fraught with technical difficulties, and gastrointestinal investigation may ...19911882265
[non-groupable streptococci: identification, sensitivity to antibiotics (charles nicolle hospital in tunis)].50 strains of viridans streptococci isolated from human material are identified by biochemical tests (bile esculin, cl na 6,5%, acid production from lactose, mannitol, sorbitol, inulin, arginine, esculin and starch hydrolysis; production of levan and dextran in sucrose media) streptococcus salivarius and streptococcus mitis are predominant species. susceptibility to antibiotics was studied: 70% of viridans streptococci were susceptible to all antibiotics tested, high level resistance to aminogly ...19911895917
on salivary film formation and bacterial retention to solids. a methodological and experimental in vitro study.a parallel-plate flow cell system was developed to assess individual and combined parameters relevant to oral microbial retention. in this system, internal reflection germanium prisms represented oral solid surfaces including tooth surfaces. the system used, allowed adsorbed protein films to be analyzed in situ by multiple non-destructive surface analytical techniques, without provoking surface associated biofilms. using a range of biochemical and biophysical techniques, germanium prisms of medi ...19911896924
changes in chemical composition and sensory qualities of peanut milk fermented with lactic acid bacteria.the effects of fermentation of aqueous extracts of peanuts (peanut milk) with lactobacillus delbrueckii ssp. bulgaricus and streptococcus salivarius ssp. thermophilus, separately and in combination, on selected chemical and sensory qualities were investigated. changes in ph, titratable acidity and viable cell populations indicated that there was a synergistic interaction between l. delbrueckii ssp. bulgaricus and s. salivarius ssp. thermophilus during fermentation. analysis of headspace volatile ...19911911084
swedish study group. a randomized multicenter trial to compare the influence of cefaclor and amoxycillin on the colonization resistance of the digestive tract in patients with lower respiratory tract infection.eighty-four patients with lower respiratory tract infections participated in a randomised double-blind parallel multicenter trial in order to compare the efficacy of cefaclor and amoxycillin as treatment for lower respiratory tract infections and their ability to influence colonization resistance. cefaclor was given to 40 patients and amoxycillin to 44 patients perorally in doses of 250 mg t.i.d. for seven days in a double-blind fashion. sputum, oropharyngeal and intestinal specimens were taken ...19911917031
molecular cloning of the extracellular endodextranase of streptococcus salivarius.we report the cloning in escherichia coli of the gene encoding an extracellular endodextranase (alpha-1,6-glucanhydrolase, ec 3.2.1.11) from streptococcus salivarius pc-1. recombinants from a s. salivarius pc-1-lambda zap ii genomic library specifying dextranase activity were identified as plaques surrounded by zones of clearing on blue dextran agar. one such clone, pd1, had a 6.3-kb ecori fragment insert which encoded a 190-kda protein with dextranase activity. the recombinant strain also produ ...19911938938
ecology of viridans streptococci in the oral cavity and pharynx.recently published taxonomic studies of viridans streptococci have resulted in several changes in the nomenclature and definition of oral streptococcal species. with this background, the ecology of streptococci in the oropharyngeal cavities was reinvestigated. the results based on the examination of 1426 streptococcal isolates confirmed and extended earlier findings. apart from mature supragingival plaque, which contained a mixture of all orally encountered streptococci, each site showed a chara ...19911945494
strains and species of lactic acid bacteria in fermented milks (yogurts): effect on in vivo lactose digestion.lactose in yogurt with live bacteria is better tolerated than lactose in other dairy foods, partly because of the activity of microbial beta-galactosidase (beta-gal), which digests lactose in vivo. to evaluate the ability of different strains and species of lactic acid bacteria to digest lactose in vivo, yogurts (containing mixtures of strains of streptococcus salivarius subsp thermophilus and lactobacillus delbrueckii subsp bulgaricus) and fermented milks (containing individual species of s the ...19911957819
the structural analysis of a levan produced by streptococcus salivarius ss2.the structure of a water-soluble levan produced by streptococcus salivarius ss2 has been determined by means of various chemical and instrumental methods. methylation and periodate oxidation studies demonstrate that the levan is comprised of d-fructofuranosyl backbone residues linked beta-(2----6) (about 70%) with beta-(2----1) branches (about 30%). 13c-n.m.r. spectral analysis of the polymer is consistent with the structure determined by chemical means.19901964870
an in vitro method to study the adherence of bacteria to saliva-treated tooth enamel sections.an in vitro bacterial adherence assay which employed human tooth enamel sections precoated with saliva and an epifluorescent staining technique with acridine orange was developed. the assay was used to study the adherence properties of fresh and type strains of the following oral bacterial species: bacteroides gingivalis, bacteroides intermedius, capnocytophaga species, haemophilus aphrophilus, actinobacillus actinomycetemcomitans, peptostreptococcus species, veillonella species, actinomyces isr ...19902087341
salivary iga antibody to oral streptococcal antigens in predentate infants.salivary iga antibodies to oral streptococci which colonize the infant oral cavity soon after birth were analyzed in 53 whole salivas of 17 infants who were between three and 27 weeks old. antibody activities to streptococcus mitis cells were detected by enzyme linked immunosorbent assay in 78% of the whole salivas by the twelfth week of age. this antibody activity was associated with polymeric iga as determined after gel filtration of salivas on superose 6, followed by elisa. western blot analy ...19902087350
a iiiman protein is involved in the transport of glucose, mannose and fructose by oral streptococci.we show in this article that the transport of glucose, mannose and fructose by the phosphoenolpyruvate: mannose phosphotransferase system of oral streptococci requires the participation of a protein component that we have called iiiman. this protein was purified from streptococcus salivarius by chromatography on deae-cellulose, deae-tsk, hydroxyapatite, and dyematrex green a. the purified protein migrated as a 38,900 molecular weight protein on a sodium dodecyl sulfate polyacrylamide gel. howeve ...19902098704
control of sugar utilization in oral streptococci. properties of phenotypically distinct 2-deoxyglucose-resistant mutants of streptococcus salivarius.the physiological and biochemical characterization of streptococcus salivarius mutants isolated by positive selection for resistance to 0.5 mm 2-deoxyglucose in the presence of lactose are reported. we found 2 classes of mutants following a series of experiments that included: growth rate determinations, uptake studies, measurement of phosphotransferase system (pts) activities and detection of the iiiman proteins by western blotting and analysis of [32p]pep-phosphorylated proteins. class 1 mutan ...19902098716
ph regulation of urease levels in streptococcus salivarius.potential mechanisms for regulation of urease levels in streptococcus salivarius were examined, including: induction by urea, nitrogen or carbon source repression, and effects of ph and co2 (because co2 enrichment enhanced urease detection on urea agar plates). regulation by either ph or co2 was confirmed by comparison of the urease accumulation pattern during anaerobic growth under co2 with that under n2. under co2, there was an initial buffering plateau at ph 6.2 and a rate of streptococcus sa ...19902110582
secretion of fructosyltransferase by streptococcus salivarius involves the sucrose-dependent release of the cell-bound form.three strains of streptococcus salivarius including a recent clinical isolate were found to possess ca2(+)-dependent fructosyltransferase (ftf) activity. the extracellular ftf activity of cells grown on sucrose increased as much as 9-fold compared with cells grown on either glucose, fructose or galactose. this increase in activity was due not to induction of ftf by sucrose, but to the release of the cell-bound form of the enzyme. studies with washed cells of s. salivarius atcc 25975 showed that ...19902141067
the influence of sucralose on bacterial metabolism.sucralose (1',4',6' trideoxy-trichloro-galactosucrose) is a nontoxic, intensely sweet sucrose derivative that has been shown to be non-cariogenic in experimental animals. the purpose of this study was to determine whether certain oral bacteria could utilize sucralose. sucralose, as a sole carbon source, was unable to support growth of ten strains of oral bacteria and dental plaque. when sucrolose was incorporated into a liquid medium containing glucose or sucrose, all organisms tested displayed ...19902143512
[the effect of zinc chloride mouthwashes on caries-inducing plaque streptococci. 1. in vitro research on the antimicrobial efficacy of zinc chloride on reference strains of streptococcus mutans (bht), streptococcus sanguis (hkop) and streptococcus salivarius (nctn 8618)].the antimicrobial efficiency of zinc chloride solution in concentration from 0.1% to 2.0% and 0.2% chlorhexidine solution has been examined on the strains of reference in a comparative study. three test methods were applied: the agar diffusion test, the dilution test and the suspension test. as was to be expected the antibacterial effect of the zinc chloride solution was dependent on concentration. between 0.1% and 2.0% the zinc chloride solution showed a bacteriostatic effect on the strains of ...19902146832
[the effect of zinc chloride mouthwashes on caries-inducing plaque streptococci. 3. the antibacterial effect of zinc chloride on the species str. mutans, str. sanguis and str. salivarius in dental plaque].the quantitative behaviour of streptococcus mutans, streptococcus sanguis and streptococcus salivarius has been examined in initial findings and after application of 0.4% zinc chloride solution. after a 7 day rinsing the number of colony developing units of the species streptococcus mutans and streptococcus sanguis in the plaque decreased to about 30% of the determined initial values. the proportions of the species streptococcus mutans and streptococcus sanguis in the total streptococcus flora o ...19902149904
glucosyltransferase production by streptococcus sanguis challis and comparison with other oral streptococci.glucosyltransferase (gtf) activity in batch cultures of streptococcus sanguis strain challis in defined medium was maximum at peak growth and declined rapidly on further incubation into stationary phase. activity was present in spent culture medium and was associated with 2 polypeptides of approximate mrs 170 kda (90% of activity) and 155 kda (about 10% of activity). other s. sanguis strains produced similar antigenically-related polypeptides but with varying activities, and antibodies to the s. ...19902150879
oral adhesion of yeasts.oral adhesion of yeasts probably occurs by interaction between yeast cell adhesins and oral epithelial cell receptors. in candida albicans mannoprotein, glucan, chitin, cell wall proteins, and lipids are possible adhesins. mannoprotein appears as a fibrillar or floccular outermost layer in stationary-phase cells grown in sugar-rich medium. preincubation of buccal epithelial cells (becs) with concanavalin a inhibits adhesion, as does suppression of mannoprotein production by tunicamycin. germ tub ...19902181810
coaggregation of streptococcus sanguis and other streptococci with candida albicans.thirteen strains of viridans group streptococci and two strains of other streptococci were tested for coaggregation with candida albicans. streptococcus sanguis strains generally exhibited low levels of adherence to 28 degrees c-grown exponential-phase yeast cells, but starvation of yeast cells for glucose at 37 degrees c (or at 28 degrees c) increased their coaggregating activity with these streptococci by at least tenfold. this was a property common to four c. albicans strains tested, two of w ...19902182544
direct observations of cooperative effects in oral streptococcal adhesion to glass by analysis of the spatial arrangement of adhering bacteria.the spatial arrangement of two strains of oral bacteria adhering on glass was studied in order to investigate cooperative effects in their adhesion mechanisms. streptococcus salivarius hb was a strain which possessed several classes of fibrillar surface appendages, whereas on the cell surface of s. mutants ns no surface appendages could be identified. the bacteria were deposited from a flowing suspension with various buffer concentrations on the bottom glass plate of a parallel plate flow cell a ...19902210339
ability to bind salivary alpha-amylase discriminates certain viridans group streptococcal species.a collection of 144 viridans group streptococcal strains recently characterized as part of a taxonomic study was examined for the ability to bind salivary alpha-amylase. this property was found in most strains of streptococcus gordonii and streptococcus mitis and in occasional strains of streptococcus anginosus and streptococcus salivarius. in contrast, all strains of streptococcus sanguis, streptococcus oralis, streptococcus vestibularis, and streptococcus mutans lacked alpha-amylase-binding ca ...19902254435
molecular genetics of streptococcus thermophilus.the metabolism and genetics of streptococcus thermophilus (presently streptococcus salivarius ssp. thermophilus) have only been investigated recently despite its widespread use in milk fermentation processes. the development of recombinant dna technology has allowed impressive progress to be made in the knowledge of thermophilic dairy streptococci. in particular, it has permitted a careful analysis of phenotypically altered variants which were derived from a mother strain by plasmid or chromosom ...19902271225
comparison of the initial streptococcal microflora on dental enamel in caries-active and in caries-inactive individuals.this study compared the initial (4 h) microflora on enamel in 7 caries-active and in 7 caries-inactive adolescents. in both groups the microflora was dominated by streptococci which comprised 61 and 78% (median values) of the total viable counts in caries-active and caries-inactive individuals, respectively (p less than 0.01). identification of a total of 700 streptococcal isolates according to a recently revised classification showed that the predominant streptococci belonged to the species str ...19902276164
cohesion between oral streptococci and neisseria pharyngis on saliva-coated glass, in the presence and absence of sucrose.ten strains of oral streptococci, comprising nine species, were screened for their in vitro cohesive interactions with a freshly isolated strain of neisseria pharyngis. strains of mutans streptococci, streptococcus salivarius, streptococcus sanguis i, streptococcus milleri and streptococcus mitis were able to cohere with n. pharyngis cells attached to a salivary pellicle adsorbed to glass. the cohesion was either due to coaggregation between the different genera or modification of the salivary p ...19902329945
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