the nature of arginine auxotrophy in cutaneous populations of staphylococci.l-arginine was required for growth by a high percentage of strains of staphylococcus species that were niche-specific and/or host-specific, but was usually not required for growth by species showing a wide host range. growth stimulation patterns with arginine intermediates indicated that most of the auxotrophic strains had blocks in an early step(s) in arginine biosynthesis. these strains were designated phenotypically as arg(chg) according to the salmonella typhimurium classification scheme. st ...1979438776
characterization and identification of coagulase-negative, heat-stable deoxyribonuclease-positive staphylococci.various characteristics of 13 coagulase-negative, weakly heat-stable deoxyribonuclease-positive staphylococci from human, veterinary and food sources were determined in an effort to identify them. nine of the isolates were identified as coagulase-negative staphylococcus aureus (2), staphylococcus xylosus (2), staphylococcus simulans (3), staphylococcus capitis (1) and staphylococcus sciuri subsp. lentus (1); the other four isolates, from food and veterinary sources, could not be identified as cu ...1978641525
prospective study of blood culture during transesophageal ascertain the incidence and significance of bacteremia associated with transesophageal echocardiography (tee), 132 consecutive patients (aged 17 to 73 years) free of apparent infection who were undergoing 135 transesophageal echocardiographic procedures from october 1990 to august 1991, were prospectively studied. for each procedure, two sets of blood cultures were obtained for culture 30 to 60 minutes before tee, immediately after, and 180 to 240 minutes after the procedure. for each blood c ...19921462911
the influence of salt concentration on the detection of methicillin resistance in coagulase-negative staphylococci.the detection of methicillin resistance by the breakpoint method was examined using three different media containing varying quantities of added salt and 4 mg/l methicillin or 1 mg/l oxacillin. three hundred clinical isolates of eight species of coagulase-negative staphylococci were tested. in 68 strains methicillin resistance was expressed only at certain salt concentrations and four distinct susceptibility phenotypes were observed. a correlation between the susceptibility phenotype and the spe ...19921493978
native valve endocarditis caused by staphylococcus simulans. 19921597208
species identification and some characteristics of coagulase-negative staphylococci isolated from bovine udders.the species of 203 strains of coagulase-negative staphylococci (cns), isolated from bovine udder quarters was determined; all were tested for hydrophobicity and encapsulation, attributes that may relate to virulence. twelve species were identified, of which staphylococcus simulans, (34.5%), s. chromogenes (16.7%), s. epidermidis (13.8%) and s. xylosus (8.9%) were the most frequent. the majority of strains possessed a hydrophilic cell surface. however, strains from two species (s. chromogenes and ...19921626368
plasmids encoding for erythromycin ribosomal methylase of staphylococcus epidermidis and staphylococcus simulans.two 1.7 md plasmids of staphylococcus epidermidis and three ones of staphylococcus simulans determining inducible macrolide-lincosamide resistance are identical as judged by restriction endonuclease fingerprinting. these plasmids designated pei2101, pei9105, pe1107, pei1108 and pei6104, respectively, belong to the incompatibility group 12. dot-blot hybridization by photobiotin-labelled gene probe developed from s. aureus erythromycin ribosomal methylase gene showed cross hybridization between me ...19921632202
homology of meca gene in methicillin-resistant staphylococcus haemolyticus and staphylococcus simulans to that of staphylococcus aureus.a penicillin-binding protein of molecular weight 76,000 inducible by beta-lactams was detected in methicillin-resistant staphylococcus haemolyticus and staphylococcus simulans. dna from these strains hybridized to the meca gene from staphylococcus aureus; however, the chromosomal hindiii fragments containing the meca genes were 3.4 kilobases in s. haemolyticus and 4.3 kilobases in s. simulans.19901691614
evaluation of the staph-zym system with staphylococci isolated from bovine intramammary infections.a total of 148 staphylococci isolated from bovine intramammary infections were used to evaluate the staph-zym system (rosco, taastrup, denmark). the overall accuracy of the system was 91.9%. the system correctly identified all strains of staphylococcus aureus, staphylococcus simulans, and staphylococcus xylosus and 95% of staphylococcus intermedius strains. of 33 staphylococcus hyicus strains, 31 (93.9%) were classified correctly by the staph-zym system, as well as 8 (80%) of 10 staphylococcus c ...19911993769
beta-lactamase is encoded on plasmid pack3 in staphylococcus simulans biovar staphylolyticus.staphylococcus simulans biovar staphylolyticus, the lysostaphin-producing organism, contains five plasmids designated pack1 through pack5. hybridization analysis using cloned beta-lactamase gene (bla) as probe and characterization of cured strains revealed that bla resides on pack3 rather than on the lysostaphin endopeptidase plasmid (pack1) as reported by others.19912004692
a case of staphylococcus simulans endocarditis affecting a native heart valve. 19912026900
a convenient method for differentiation of coagulase-negative staphylococci isolated from bovine mammary glands.the utility of trehalose-mannitol broth and arabinose-cellobiose broth for identification of staphylococcus epidermidis and novobiocin-resistant staphylococci was determined using 236 coagulase-negative staphylococci isolated from bovine mammary glands. none of the 49 s. epidermidis strains was positive in trehalose-mannitol broth; whereas, all strains of staphylococcus hyicus, staphylococcus chromogenes, staphylococcus haemolyticus, staphylococcus hominis, staphylococcus warneri, and staphyloco ...19912045549
classification of coagulase-negative staphylococci isolated from bovine clinical and subclinical hundred and forty eight strains of coagulase-negative staphylococci (cns) from bovine clinical and subclinical mastitis were classified by conventional biochemical testing. staphylococcus simulans was the most frequently isolated species. the distribution of cns species in the two groups was similar regardless of whether the cow suffered from clinical or subclinical mastitis. there was no significant difference between the grades of severity of the clinical mastitis associated with the vario ...19912063547
presence of an additional penicillin-binding protein in methicillin-resistant staphylococcus epidermidis, staphylococcus haemolyticus, staphylococcus hominis, and staphylococcus simulans with a low affinity for methicillin, cephalothin, and cefamandole.the presence of an additional penicillin-binding protein (pbp) was demonstrated in methicillin-resistant strains of staphylococcus epidermidis, s. haemolyticus, s. hominis, and s. simulans. in these four species, the apparent molecular mass of this protein was analogous to that of pbp 2' of methicillin-resistant s. aureus sr 1550-9. it exhibited a low affinity for methicillin, cephalothin, and cefamandole; and its synthesis was methicillin inducible. peptide mapping of this pbp from the four spe ...19902285281
effect of milk on fibronectin and collagen type i binding to staphylococcus aureus and coagulase-negative staphylococci isolated from bovine mastitis.tryptic soy broth (tsb)-grown cells of staphylococcus aureus isolated from acute and chronic bovine mastitis bound mainly 125i-fibronectin (fn) [corrected], whereas strains of nine species of coagulase-negative staphylococci showed a predominant interaction with 125i-collagen (cn) [corrected] type i. a particle agglutination assay (paa) was used to examine the interaction of coagulase-negative staphylococci with 125i-fn and 125i-cn immobilized on latex. all 368 coagulase-negative staphylococci d ...19892775349
recognition of staphylococcus saprophyticus in urine cultures by screening colonies for production of phosphatase.phenolphthalein diphosphate was incorporated into a primary blood agar medium for use in performing quantitative urine cultures. phosphatase-negative staphylococci, such as staphylococcus saprophyticus, were differentiated from phosphatase-positive species, such as staphylococcus epidermidis, by spot testing colonies on filter paper saturated with 1 n naoh. phosphatase-positive colonies turned pink within seconds, and phosphatase-negative colonies showed no color. none of 55 s. saprophyticus iso ...19852984240
characteristics of extracellular protein production by staphylococcus simulans biovar staphylolyticus during aerobic and anaerobic growth.aerobic cultures of staphylococcus simulans biovar staphylolyticus characteristically achieved about 17 times higher bacterial densities and produced about 7 times higher concentrations of exoprotein than did anaerobic cultures. however, total exoprotein secreted per unit of bacterial dry weight typically was 2.3 times greater for anaerobic cultures. as determined by sds-page, anaerobic cultures also produced a wider variety of exoproteins than did aerobic cultures. three exoenzymes, a staphylol ...19883076190
lack of pleiotropic compensation in extracellular protein production by hypoproducing variants of staphylococcus simulans biovar staphylolyticus.the changes in bacterial density, total extracellular protein and activities of three extracellular enzymes were monitored during growth of wild-type staphylococcus simulans biovar staphylolyticus, a representative pleiotropic variant that produced decreased levels of the three extracellular enzymes, and a variant that produced only 5 of the 14 extracellular proteins secreted by the wild-type organism. both variants produced less total extracellular protein than did the parental organism. sds-pa ...19873309147
cloning, sequence, and expression of the lysostaphin gene from staphylococcus simulans.a 1.5-kilobase-pair fragment of dna that contains the lysostaphin gene from staphylococcus simulans and its flanking sequences has been cloned and completely sequenced. the gene encodes a preproenzyme of mr 42,000. the nh2-terminal sequence of the preproenzyme is composed of a signal peptide followed by seven tandem repeats of a 13-amino acid sequence. conversion of prolysostaphin to the mature enzyme occurs extracellularly in cultures of s. simulans and involves removal of the nh2-terminal port ...19873547405
simple screening method for identification of nonpleiotropic mutants for exoenzyme production.a differential medium that distinguishes between pleiotropic and nonpleiotropic mutants for exoenzyme production has been developed for staphylococcus simulans biovar staphylolyticus. the medium will facilitate genetic analysis of exoenzyme production by this organism. generally useful strategies for increasing the sensitivity of indicator plates for detection of exoenzyme activities are presented.19854004249
distribution of staphylococcal species on clinically healthy cats.among 827 isolates derived from 113 clinically healthy cats, 12 species of staphylococci were identified. staphylococci were isolated from each cat and from 54.9% of the anatomic sites evaluated. a mode of 6 (range = 2 to 11) of the 11 anatomic sites evaluated per cat yielded staphylococci. a mode of 8 (range = 2 to 12) isolates were found per cat. staphylococcus simulans was the most isolated (43.9% of total) coagulase-negative species. moreover, s simulans was the most isolated species from ea ...19854051289
resistance plasmids for inducible macrolide-lincosamide resistance in staphylococcus simulans and staphylococcus epidermidis.localization and genetic nature of inducible macrolide resistance determinants in some coagulase negative staphylococci (cns) were studied and compared with those in staphylococcus aureus. an 1.7-megadalton r-plasmid, pei 1107, mediating inducible resistance to macrolides and lincosamides was present in part of staphylococcus simulans and staphylococcus epidermidis strains isolated in the central hospital for infectious diseases. two other plasmids were harboured by s. simulans lk 6108 strain. o ...19854082898
[drug susceptibility of staphylococci isolated from clinical specimens].eighty-eight strains of staphylococci were isolated from a variety of specimens collected from patients at our university hospital from june to august 1982. these strains were identified as staphylococcus aureus (86%), staphylococcus epidermidis (6.8%), staphylococcus haemolyticus (2.3%), staphylococcus capitis (2.3%), and staphylococcus simulans (1.1%). one strain could not be identified with certainty as a currently recognized species. a majority (70%) of the s. aureus strains was resistant to ...19854089343
effects of amino acids and derivatives of cyclic adenosine 3', 5'-monophosphate on the production of three exoenzymes by staphylococcus simulans biovar staphylolyticus. 19826297487
biochemical and physical properties of the endo-beta-n-acetylglucosaminidases from staphylococcus aureus, staphylococcus simulans, and staphylococcus saprophyticus.biochemical and physical properties of the pure bacteriolytic enzymes excreted by three different staphylococcus species (s. aureus, s. simulans, and s. saprophyticus) were investigated. although the three enzymes have previously been shown to share the same specificity of action (endo-beta-n-acetylglucosaminidase activity), their biochemical features clearly indicated that they were three different enzymes, confirming what had previously been suggested by the different lytic-activity patterns d ...19836420648
identification and characterization of staphylococci isolated from cats.staphylococcus simulans was found to be the most common staphylococcal species present on the healthy skin and in the nares of cats. s. aureus was the most frequent staphylococcus species in lesions. most of the s. aureus strains showed the characteristics of the human ecovar. other strains isolated formed a heterogenic collection in which not less than 13 species were represented. one of these, s. epidermidis, was almost exclusively associated with lesions. it was concluded that coagulase-posit ...19846474798
chocolate agar, a differential medium for gram-positive cocci.reactions incurred on chocolate agar by gram-positive cocci were correlated with species identity. darkening and clearing of the medium was usually associated with the species staphylococcus epidermidis, staphylococcus aureus, staphylococcus simulans, and streptococcus faecalis. yellowing of chocolate agar was associated with alpha-hemolytic species of streptococcus. the study demonstrated that reactions occurring on chocolate agar are useful in identifying gram-positive cocci.19846490866
identification, clinical distribution, and susceptibility to methicillin and 18 additional antibiotics of clinical staphylococcus isolates: nationwide investigation in italy.a multicentric study of clinical staphylococcus isolates was performed by seven operative units working in different areas of italy. over a 6-month period, a total of 3,226 staphylococci, isolated from in- and outpatients, were identified and tested for antimicrobial susceptibility by a protocol agreed upon by all units. on the basis of their bacteriolytic-activity patterns and other conventional tests, the isolates were identified by lyogroups , which closely correlate with human staphylococcus ...19846565709
recruitment of neutrophils by an encapsulated coagulase-negative strain of staphylococcus simulans in the mammary gland of the mouse.the encapsulated coagulase-negative strain of staphylococcus simulans (strain 76) was inoculated into the mammary glands of lactating mice. in contrast to the coagulase-positive encapsulated staphylococcus aureus (strain m), which elicited a neutrophil response within 18 hr, strain 76 organisms multiplied in the glands but failed to elicit a neutrophil response for 3 days; they were then eliminated from the gland by 8 days. when strain 76 was inoculated into mice whose offspring had been removed ...19846593392
purification and characterization of three separate bacteriolytic enzymes excreted by staphylococcus aureus, staphylococcus simulans, and staphylococcus a further development of previous investigations showing that different staphylococcal species display different bacteriolytic activity patterns (lyogroups), the bacteriolytic enzymes excreted by three different staphylococcus species, staphylococcus aureus (lyogroup i), s. simulans (lyogroup ii), and s. saprophyticus (lyogroup iv); have been purified and characterized. a representative strain from each species was grown in a preselected medium made of fully dialyzable products. culture super ...19826807958
characterization of coagulase-negative staphylococci from urinary tract specimens.species of coagulase-negative staphylococci isolated from urine specimens submitted from both inpatients and outpatients to the clinical microbiology laboratory of a teaching hospital were identified with a biotyping system, with species then correlated by clinical features and antimicrobial susceptibility. of 145 isolates, 102 (70%) were staphylococcus epidermidis, 24 (17%) were staphylococcus saprophyticus, 7 (4.7%) were staphylococcus haemolyticus, 4 (2.8%) were staphylococcus hominis, 3 (2.1 ...19836833480
encapsulated, coagulase-negative strain of staphylococcus encapsulated strain of staphylococcus simulans (strain 76) isolated from a clinical case of bovine mastitis was demonstrated by the india ink technique, using both a light microscope and an electron microscope. the strain failed to kill mice after intraperitoneal inoculation but resisted ingestion by peritoneal phagocytic cells. the behavior of the strain helps to delineate the antiphagocytic role of the capsule.19817263067
a polypeptide antigen from a strain of staphylococcus simulans. 1. purification and some chemical data.a polypeptide antigen isolated from staphylococcus simulans was shown mainly to contain alanine and aspartic acid in a molar ratio of 3:1. the circular dichroism spectre showed only positive ellipticities, and oxidation by d-amino acid oxidase showed the presence of d-alanine. 65 per cent of the alanine was present as the d-isomer as estimated by gas-liquid chromatography. analytical ultracentrifugation showed that the polymer sedimented as a homogeneous peak with an s20, w value of 2.8.19817315344
identification of a toxr-activated gene, tage, that lies within the accessory colonization factor gene cluster of vibrio cholerae o395.the nucleotide (nt) sequence has been determined for a vibrio cholerae toxr-activated gene designated tage that is located within a cluster of genes required for efficient intestinal colonization. the tage gene encompasses 909 nt and is predicted to encode a 303-amino-acid (aa) protein with an estimated molecular mass of 34,468 da. computer-assisted similarity searches revealed that tage possesses aa sequence similarity with escherichia coli orfu and staphylococcus simulans lysostaphin, two prot ...19947523253
mode of action of the lantibiotic mersacidin: inhibition of peptidoglycan biosynthesis via a novel mechanism?mersacidin is an antibiotic peptide produced by bacillus sp. strain hil y-85,54728 that belongs to the group of lantibiotics. its activity in vivo against methicillin-resistant staphylococcus aureus strains compares with that of the glycopeptide antibiotic vancomycin (s. chatterjee, d. k. chatterjee, r. h. jani, j. blumbach, b. n. ganguli, n. klesel, m. limbert, and g. seibert, j. antibiot. 45:839-845, 1992). incubation of staphylococcus simulans 22 with mersacidin resulted in the cessation of g ...19957793878
distribution of coagulase-negative staphylococci, including the newly described species staphylococcus schleiferi, in nosocomial and community acquired urinary tract infections.four hundred and four coagulase-negative staphylococci were isolated from 4905 urine specimens obtained from 4192 inpatients and outpatients. the distribution of the strains was as follows: 193 staphylococcus epidermidis (47.8%), 171 staphylococcus saprophyticus (42.3%), 29 staphylococcus haemolyticus (7.2%), 5 staphylococcus warneri (1.2%), 3 staphylococcus schleiferi (0.7%), 2 staphylococcus hominis (0.5%) and 1 staphylococcus simulans (0.2%). all three staphylococcus schleiferi strains were i ...19947889974
characterization of the most frequently encountered staphylococcus sp. in cats.ninety three staphylococci isolated from clinical specimens from cats were characterized and identified. because the biochemical characteristics of staphylococcus felis were very similar to those of staphylococcus simulans, results were submitted to numerical analysis and dna homology. forty-two isolates (45%) were identified as s. felis, and 4 isolates (4%) as s. simulans. the other species identified, in order of their frequency were, 12 staphylococcus aureus (13%), 9 staphylococcus intermediu ...19948042273
extracellular proteolytic activation of bacteriolytic peptidoglycan hydrolases of staphylococcus simulans biovar staphylolyticus.staphylococcus simulans biovar staphylolyticus secreted two bacteriolytic peptidoglycan hydrolases as proproteins that were activated as they were processed by an extracellular sulphydryl protease. this processing resulted in the production of multiple molecular-mass forms of each enzyme. cells from early exponential phase cultures were susceptible to lysis by the mature forms of each of the peptidoglycan hydrolases whereas stationary phase cells were resistant. thus secretion of these bacteriol ...19938102340
pubic osteomyelitis caused by staphylococcus simulans. 19938121867
expression of the lysostaphin gene of staphylococcus simulans in a eukaryotic system.the lysostaphin gene of staphylococcus simulans was cloned into escherichia coli. the 5' end of the gene was modified to include a eukaryotic start codon, the kozak expression start site consensus sequence, and an enzyme site to facilitate manipulation of the gene. transcription of the modified gene in vitro yielded an rna transcript which, when added to a rabbit reticulocyte cell-free translation system, directed the synthesis of several products. the largest product, migrating at approximately ...19948161174
use of liposome-immunopotentiated exopolysaccharide as a component of an ovine mastitis staphylococcal vaccine.experiments on the development of a vaccine against staphylococcal mastitis were carried out in ewes. the vaccine (spanish patent no. 9200223) has the following components: (i) inactivated (formalinized) bacteria (staphylococcus aureus and a coagulase-negative staphylococcal species. staphylococcus simulans) and s. aureus toxoid in presence of an adjuvant (dextran sulfate, mw 500,000); and (ii) s. aureus exopolysaccharide included within liposomes. high serum antibody titres were obtained agains ...19948165857
oral crohn's disease and pyostomatitis vegetans. an unusual association.oral features of crohn's disease include ulcers, lip fissuring, cobblestone plaques, angular cheilitis, polypoid lesions, and perioral erythema. pyostomatitis vegetans is a rare eruption of the oral mucosa characterized by tiny yellow pustules. it is considered a marker for inflammatory bowel disease. we describe a 45-year-old woman with a 6-month history of painful sores in her mouth, diarrhea, weight loss, and cutaneous lesions. oral examination revealed cobblestone plaques and indentation on ...19938426722
efficient adsorption of lysostaphin on bacterial cells of lysostaphin-resistant staphylococcus aureus mutant.a simple and efficient method for the purification of staphylolytic endopeptidase (lysostaphin) contained in culture supernatant of staphylococcus simulans biovar staphylolyticus strain by adsorption of the enzyme on bacterial cells of lysostaphin-resistant s. aureus mutant was successfully devised. lysostaphin was sufficiently absorbed on the heat-killed mutant cells derived from s. aureus cowan i and efficiently eluted by 3 m kscn. enzyme preparation obtained by a single procedure of the affin ...19938474355
syndrome of inappropriate secretion of antidiuretic hormone in elderly patients with rheumatoid arthritis associated with infections: report of two cases.two rheumatoid arthritis (ra) patients with the syndrome of inappropriate secretion of antidiuretic hormone (siadh) during the course of infection are herein reported. one patient developed siadh during the course of a localized cutaneous herpes zoster infection while the other developed siadh in conjunction with staphylococcus simulans septicemia. we consider that the development of siadh was strongly associated with superimposed infections in the underlying ra. this is the first report discuss ...19968835600
identification of micrococcaceae isolated from goat's milk and cheese in the poitou-charentes hundred and ninety strains of coagulase-negative staphylococci were isolated from goat's milk, whey and cheese at various stages of manufacture. sixteen different coagulase negative staphylococci (cns) species were recovered, 3 of which were predominant: staphylococcus simulans, staphylococcus epidermidis and staphylococcus xylosus. the prevalent species were recovered at least at two different stages of cheese manufacturing, suggesting a better adaptation to the environment. after 15 days o ...19968854188
target cell specificity of a bacteriocin molecule: a c-terminal signal directs lysostaphin to the cell wall of staphylococcus aureus.microbial organisms secrete antibiotics that cause the selective destruction of specific target cells. although the mode of action is known for many antibiotics, the mechanisms by which these molecules are directed specifically to their target cells hitherto have not been described. staphylococcus simulans secretes lysostaphin, a bacteriolytic enzyme that cleaves staphylococcal peptidoglycans in general but that is directed specifically to staphylococcus aureus target cells. the sequence element ...19968890152
methicillin-resistant staphylococcus simulans masquerading as mrsa in a nursing home. 19968971622
prevalence and duration of intramammary infection in danish heifers during the peripartum period.milk samples were collected from 180 heifers from 20 herds around parturition to study the prevalence and persistence of intramammary infection (imi). a total of 3382 quarter milk samples was collected each week from 4 wk before parturition to 4 wk after parturition. staphylococcus chromogenes, the most commonly found bacterial species before parturition, was isolated from 15% of all quarters. however, imi with staph. chromogenes decreased shortly after parturition to around 1% of quarters. infe ...19979058272
identification of staphylococcal and streptococcal causes of bovine mastitis using 16s-23s rrna spacer regions.bovine mastitis is caused mainly by certain staphylococcus and streptococcus species. the sequences of the 16s-23s rrna spacer regions were determined for the nine species which cause mastitis: staphylococcus aureus, staphylococcus chromogenes, staphylococcus epidermidis, staphylococcus hyicus, staphylococcus simulans, staphylococcus xylosus, streptococcus agalactiae, streptococcus dysgalactiae and streptococcus uberis. significant variation was found between the spacer sequences of different sp ...19979387227
in vivo presence of capsular polysaccharide in coagulase-negative staphylococci of ovine origin.three strains of coagulase-negative staphylococci identified as staphilococcus warnery, staphylococcus simulans and staphylococcus haemolyticus were examine for evidence of capsular polysaccharide, in vitro by negative staining with india ink, and in vivo by transmission electron microscopy. in vitro, unstained materials surrounded clusters or single cocci. in vivo, capsula materials were surrounding phagocytosed bacteria cells. these capsular materials were either closely or loosely bound to ba ...19989497929
isolation of coagulase-negative staphylococci from the milk and environment of sheep.various species of coagulase-negative staphylococci (c-ns) are reported to be common in milk and on the teat skin of domestic ruminants. the commonest c-ns species in mastitic milk of cows varies between reports, with staphylococcus simulans (jarp, 1991) in one and staph. hyicus in another (watts & washburn, 1991). the teat skin of heifers may be colonized by staph. xylosus or staph. chromogenes, while staph. chromogenes and staph. warneri are reported as frequent isolates from teat canals and s ...19989513060
regulation of agr-dependent virulence genes in staphylococcus aureus by rnaiii from coagulase-negative staphylococci.many of the genes coding for extracellular toxins, enzymes, and cell surface proteins in staphylococcus aureus are regulated by a 510-nucleotide (nt) rna molecule, rnaiii. transcription of genes encoding secreted toxins and enzymes, including hla (alpha-toxin), saeb (enterotoxin b), tst (toxic shock syndrome toxin 1), and ssp (serine protease), is stimulated, while transcription of genes encoding cell surface proteins, like spa (protein a) and fnb (fibronectin binding proteins), is repressed. be ...19989620969
lysostaphin treatment of experimental methicillin-resistant staphylococcus aureus aortic valve endocarditis.the emergence of clinical isolates of methicillin-resistant staphylococcus aureus with reduced susceptibility to vancomycin has prompted a search for new and novel therapeutic agents active against s. aureus. lysostaphin, a peptidase produced by staphylococcus simulans, specifically cleaves the glycine-glycine bonds unique to the interpeptide cross-bridge of the s. aureus cell wall. the effectiveness of various regimens of dosing with intravenous lysostaphin was compared to that of vancomycin in ...19989624475
wall-associated processing of extracellular enzymes of staphylococcus simulans biovar staphylolyticus.staphylococcus simulans biovar staphylolyticus produces a staphylolytic glycylglycine endopeptidase (lysostaphin) and a micrococcolytic endo-beta-n-acetylglucosaminidase (hexosaminidase) as proenzymes that are proteolytically processed through multiple intermediates to their mature forms by an extracellular sulfhydryl protease. analysis of protease production by immunoblots using antiserum prepared against purified protease and by renaturing activity gels using gelatin as the substrate has revea ...19989711848
plasmid profile and antibiotic resistance in coagulase-negative staphylococci isolated from polluted water.four different species of coagulase-negative staphylococci (cns) were isolated from polluted waters in fez, morocco and found to be staphylococcus simulans, staph. lenticus, staph. hyicus and staph. xylosus. eight isolates belonging to these four species were analysed for their plasmid content. southern blot hybridizations were performed to define the resistance determinants of the plasmids harboured by these species. these determinants were found to be carried mainly by class i staphylococcal p ...19989721646
influence of lif, the lysostaphin immunity factor, on acceptors of surface proteins and cell wall sorting efficiency in staphylococcus carnosus.proteins harboring a c-terminal cell wall sorting signal are covalently linked to pentaglycine acceptors within the staphylococcal peptidoglycan. this pentaglycine was modified when the lysostaphin immunity factor (lif) of staphylococcus simulans was expressed in staphylococcus carnosus, likely by the exchange of two glycine residues for serine residues. a reporter protein was efficiently linked to the modified acceptor, indicating that the sorting reaction is not strictly dependent on the wild- ...19989733703
molecular characterization of the erythromycin resistance plasmid ppv142 from staphylococcus simulans.the 2.5-kb erythromycin resistance (emr) plasmid ppv142 of staphylococcus simulans 13044 was isolated and characterized. sequence analysis identified orf1 and orf2 encoding a 158-residue replication protein (rep142) and a 244-residue erythromycin resistance protein (erm, rrna adenine n-6-methyltransferase), respectively. structural analysis and southern hybridization showed that the rep and ermm genes in ppv142 shared homology with the emr plasmid ppv141 (2.4 kb) of s. chromogenes 3688 and other ...19989742700
[deamination of adenine and adenosine in staphylococci].deaminations of adenine and adenosine by pattern strains of 24 staphylococcal species, were tested. during 3 hours of incubation of the suspensions of 8 staphylococci with adenine the liberation of ammonia occurred. the same staphylococci accumulated ammonia in the incubation medium with adenosine. the staphylococcus intermedius pcm 2405 strain as opposite to the staphylococcus aureus 536 strain in the media with adenine or adenosine accumulated hypoxanthine or inosine, respectively and ammonia. ...19979554142
minimisation of microbial contamination for potential islet xenografts using specific pathogen-free pigs and a protected environment during tissue major risk of islet xenotransplantation is transmission of infections. we thus compared microbial contamination during preparation of islets from 4 pigs conventionally breeded and slaughtered or 8 specific pathogen free (spf) pigs, and different environmental conditions during pancreas excision. pancreas harvested in a slaughterhouse (for conventional pigs) or in a protected autopsy room (for spf pigs) were soaked in betadine solution and submitted to enzymatic digestion with collagenase. is ...19979496562
characterization of staphylococcus simulans strains isolated from cases of bovine mastitis.this study was conducted to characterize staphylococcus simulans isolated from cases of bovine mastitis. a total of 134 isolates of s. simulans selected from 80 quarters from 61 cows or heifers in 37 different herds were characterized by ecori ribotyping. from 22 quarters two to seven consecutive isolates taken at weekly intervals were selected. furthermore, three isolates from clinical infections in humans and two reference strains were included. a total of 16 different ribotypes were found, ho ...199910227477
characterization of the most frequently encountered staphylococcus sp. in the rumen of deer.the total enumeration and biotyping of staphylococci isolated from the rumen content of deer is presented in this study. the total counts of staphylococcal isolates from deer reached 1.5 x 10(3) cfu/ml. of 960 colonies, 904 were taxonomically identified. coagulase-negative staphylococci mainly staphylococcus simulans and staph. xylosus were the most frequently isolated species, followed by staph. warneri, staph. lentus, staph. saprophyticus, staph. epidermidis and staph. hominis. moreover, the a ...19979313367
glyceraldehyde-3-phosphate dehydrogenase-encoding gene as a useful taxonomic tool for staphylococcus spp.the gap gene of staphylococcus aureus, encoding glyceraldehyde-3-phosphate dehydrogenase, was used as a target to amplify a 933-bp dna fragment by pcr with a pair of primers 26 and 25 nucleotides in length. pcr products, detected by agarose gel electrophoresis, were also amplified from 12 staphylococcus spp. analyzed previously. hybridization with an internal 279-bp dna fragment probe was positive in all pcr-positive samples. no pcr products were amplified when other gram-positive and gram-negat ...200011101563
molecular cloning, sequencing, and expression of lytm, a unique autolytic gene of staphylococcus aureus.a gene encoding an autolytic activity was identified in an autolysis-deficient mutant (lyt-) of staphylococcus aureus which produces only a single band in autolytic-activity gels (n. mani, p. tobin, and r. k. jayaswal, j. bacteriol. 175:1493-1499, 1993). an open reading frame, designated lytm, of 948 bp that could encode a polypeptide of 316 amino acid residues was identified. the calculated molecular mass of the lytm gene product (34.4 kda) corresponded to that of the autolytic activity detecte ...19979171409
mechanism and suppression of lysostaphin resistance in oxacillin-resistant staphylococcus aureus.the potential for the development of resistance in oxacillin-resistant staphylococcus aureus (orsa) to lysostaphin, a glycylglycine endopeptidase produced by staphylococcus simulans biovar staphylolyticus, was examined in vitro and in an in vivo model of infection. following in vitro exposure of orsa to subinhibitory concentrations of lysostaphin, lysostaphin-resistant mutants were idenitifed among all isolates examined. resistance to lysostaphin was associated with a loss of resistance to beta- ...200111302806
bacterial flora of the conjunctiva and nasal cavity in normal and diseased captive bustards.a survey was carried out to describe the normal aerobic bacterial flora of the conjunctiva and nasal cavity of captive houbara bustards (chlamydotis undulata), kori bustards (ardeotis kori), and white-bellied bustards (eupodotis senegalensis) maintained at the national avian research center, abu dhabi, united arab emirates. a total of 58 samples were examined from the nasal cavity and 55 samples from the conjunctiva of healthy bustards. there was no bacterial growth in 45% of conjunctival sample ...200111417826
studies on prolysostaphin processing and characterization of the lysostaphin immunity factor (lif) of staphylococcus simulans biovar staphylolyticus.lysostaphin is an extracellular glycylglycine endopeptidase produced by staphylococcus simulans biovar staphylolyticus atcc1362 that lyses staphylococcal cells by hydrolysing the polyglycine interpeptide bridges of the peptidoglycan. renewed analysis of the sequence of the lysostaphin gene (lss), and the sequencing of the amino-terminus of purified prolysostaphin and of mature lysostaphin revealed that lysostaphin is organized as a preproprotein of 493 amino acids (aa), with a signal peptide con ...19979106216
vertebral osteomyelitis and prosthetic joint infection due to staphylococcus simulans.staphylococcus simulans, a coagulase-negative staphylococcus, is a common animal pathogen that is rarely encountered in human infections. we describe a 70-year-old man who developed multifocal vertebral osteomyelitis and late prosthetic joint infection caused by this pathogen. the patient was a farmer who had daily contact with cows and drank unpasteurized milk, although the portal of the pathogen's entry remains speculative. culture of the vertebral disk biopsy specimen and cultures during rese ...200111605694
biochemical and molecular characterization of staphylococcus simulans lipase.staphylococcus simulans strain secretes a non-induced lipase in the culture medium. staphylococcus simulans lipase (ssl), purified to homogeneity, is a tetrameric protein (160 kda) corresponding to the association of four lipase molecules. the 30 n-terminal amino acid residues were sequenced. this sequence is identical to the one of staphylococcus aureus ps54 lipase (sal ps54) and exhibits a high degree of homology with staphylococcus aureus nctc8530 lipase (sal nctc8530), staphylococcus hyicus ...200111698108
mode of action of the antimicrobial peptide aureocin a53 from staphylococcus aureus.we investigated the mode of action of aureocin a53 on living bacterial cells and model membranes. aureocin a53 acted bactericidally against staphylococcus simulans 22, with >90% of the cells killed within a few minutes. cell death was followed by lysis, as indicated by a clearing of the cell suspension and gram staining. aureocin a53 rapidly dissipated the membrane potential and simultaneously stopped biosynthesis of dna, polysaccharides, and protein. aureocin a53 induced a rapid release of prea ...200212406714
influence of pulsation rate on udder health and teat thickness changes in dairy this work on machine milking of ewes, pulsation rates of 120 and 180 cycles per min were compared, both with a pulsation ratio of 50:50 and a vacuum level of 36 kpa, comparing intramammary infection (imi), somatic cell count (scc) and teat end thickness changes. to this end, two groups of 20 manchega ewes were used in a crossover experimental design with two experimental periods of 24 d for each. bacterial exposure of all teats was increased by dipping them in a suspension of staphylococcus s ...200312647959
purification and some properties of lysostaphin, a glycylglycine endopeptidase from the culture liquid of staphylococcus simulans biovar staphylolyticus.this work presents a method for purification of lysostaphin, a glycylglycine endopeptidase, from the culture liquid of s. simulans biovar staphylolyticus to homogeneity in a few steps. the method includes ultrafiltration and ion-exchange and hydrophobic chromatographies. the enzyme was isolated in preparative amounts with the yield of 51%. some physical and chemical properties of the enzyme are described.200312693976
microbial quality and direct pcr identification of lactic acid bacteria and nonpathogenic staphylococci from artisanal low-acid sausages.detection of six species of lactic acid bacteria and six species of gram-positive catalase-positive cocci from low-acid fermented sausages (fuets and chorizos) was assessed by species-specific pcr. without enrichment, lactobacillus sakei and lactobacillus curvatus were detected in 11.8% of the samples, and lactobacillus plantarum and staphylococcus xylosus were detected in 17.6%. enriched samples allowed the detection of l. sakei and s. xylosus in all of the samples (100%) and of enterococcus fa ...200312902246
effects of the lantibiotic mersacidin on the morphology of staphylococci.mersacidin is a lanthionine-containing peptide antibiotic (lantibiotic), able to inhibit the growth of a number of gram-positive bacteria including methicillin-resistant staphylococcus aureus (mrsa) in a manner similar to, but distinct from, vancomycin. in order to further understand the mode of action of this lantibiotic, staphylococcus simulans 22 cells were treated either with the antibiotics penicillin, tunicamycin or vancomycin or with mersacidin and then compared with untreated cells after ...19968837393
expression of the genes for lysostaphin and lysostaphin resistance in determine if the genes for lysostaphin endopeptidase (end) and lysostaphin resistance (epr) function in streptococci, we transferred these genes from staphylococcus simulans biovar staphylolyticus into two strains of streptococcus equi subsp. zooepidemicus. the end-containing streptococci were able to produce and process proendopeptidase. strains containing epr were more resistant to lysis by the streptococcolytic enzyme zoocin a and amino acid analysis of the peptidoglycans of the epr-contai ...200314612246
plasmid-encoded catalase of staphylococcus simulans biovar staphylolyticus.staphylococcus simulans biovar staphylolyticus contains five plasmids designated pack1 through pack5. non-denaturing electrophoretic analysis of an extract prepared from wild-type cells revealed three bands of catalase activity, whereas an extract of cells cured of pack1 produced only two catalase bands. cloning and southern hybridization analysis showed that there is a catalase structural gene on pack1. the plasmid-specified catalase was the major activity produced under both aerobic and anaero ...19948181729
pseudomonas aeruginosa lasa protease in treatment of experimental staphylococcal keratitis.lasa protease is a staphylolytic endopeptidase secreted by pseudomonas aeruginosa. we have examined the effectiveness of lasa protease in the treatment of staphylococcal keratitis caused by methicillin-sensitive staphylococcus aureus (mssa) and methicillin-resistant s. aureus (mrsa) isolates in a rabbit model. keratitis was induced by intrastromal injection of the bacteria. the eyes were treated topically, and the efficacy of lasa protease was compared to those of lysostaphin (a staphylolytic pr ...200415105121
the etiologic diagnosis of infectious discitis is improved by amplification-based dna analysis.blood cultures and cultures of disc material are required to identify and treat bacterial agents responsible for septic spondylodiscitis, but these methods have limited sensitivities. we undertook this study to compare nonculture amplification-based dna analysis with conventional culture of disc aspirate.200415457468
identification of coagulase-negative staphylococci other than staphylococcus epidermidis by automated routine identification of coagulase-negative staphylococci is problematic, the performance of automated ribotyping was evaluated for identification of coagulase-negative staphylococci other than staphylococcus epidermidis. in total, 177 isolates were tested, comprising 149 isolates from blood samples, 15 isolates that were not identified by internal transcribed spacer (its)-pcr in a previous study, and 13 reference strains. the identification results were compared with those obtained by the a ...200515715714
new effective sources of the staphylococcus simulans lysostaphin.the gene encoding staphylococcus simulans lysostaphin has been cloned into two escherichia coli expression systems: pet23b+ (novagen, uk) and pbad/thio-topo (invitrogen, usa), which allow the overexpression of a target protein as a fusion protein. the enzyme produced in the pet system contains a cluster of six histidines at the c-terminus, and the protein produced in the pbad system contains 133 additional amino acid residues at the n-terminus, including thioredoxin, a cluster of six histidines ...200515823409
some properties of coagulase-negative staphylococci isolated from cases of ovine mastitis.of 41 coagulase-negative staphylococcal isolates from cases of ovine mastitis, 80% were speciated by the 'api-staph system' and 90% by a combination of biochemical tests. staphylococcus simulans and staph. xylosus were the two most prevalent species.19948119356
bap-dependent biofilm formation by pathogenic species of staphylococcus: evidence of horizontal gene transfer?the biofilm-associated protein (bap) is a surface protein implicated in biofilm formation by staphylococcus aureus isolated from chronic mastitis infections. the bap gene is carried in a putative composite transposon inserted in sapibov2, a mobile staphylococcal pathogenicity island. in this study, bap orthologue genes from several staphylococcal species, including staphylococcus epidermidis, staphylococcus chromogenes, staphylococcus xylosus, staphylococcus simulans and staphylococcus hyicus, w ...200516000737
identification and differentiation of staphylococcus carnosus and staphylococcus simulans by species-specific pcr assays of soda genes.the aim of this study was to design species-specific pcr assays for rapid and reliable identification and differentiation of staphylococcus (s.) carnosus and s. simulans strains. two different sets of primers, targeting the manganese-dependent superoxide dismutase (soda) gene of s. carnosus and s. simulans, respectively, were designed. species-specificity of both sets of primers was evaluated by using 93 strains, representing 26 different species of the genus staphylococcus, 3 species of the gen ...200516106559
microbiological quality of goat's milk obtained under different production order to determine the safety of milk produced by smallholder dairy goat farms, a farm-based research study was conducted on commercial dairy goat farms to compare the microbiological quality of milk produced using 3 different types of dairy goat production systems (intensive, semi-intensive and extensive). a survey of dairy goat farms in and around pretoria carried out by means of a questionnaire revealed that most of the smallholder dairy goat farms surveyed used an extensive type of produc ...200516108524
cytoplasmic expression of mature glycylglycine endopeptidase lysostaphin with an amino terminal hexa-histidine in a soluble and catalytically active form in escherichia coli.methicillin-resistant staphylococcus aureus is a major problem in the world, causing hospital acquired infections and the infections/pathogenesis in community. lysostaphin is a novel therapeutic molecule to kill the multidrug-resistant s. aureus. mature lysostaphin is a single polypeptide (approximately 27 kda) chain metalloprotease glycylglycine endopeptidase, capable of specifically hydrolyzing penta-glycine crosslinks present in the peptidoglycan of the s. aureus cell wall. the mature lysosta ...200616181789
the lysostaphin endopeptidase resistance gene (epr) specifies modification of peptidoglycan cross bridges in staphylococcus simulans and staphylococcus aureus.staphylococcus simulans biovar staphylolyticus produces an extracellular glycylglycine endopeptidase (lysostaphin) that lyses other staphylococci by hydrolyzing the cross bridges in their cell wall peptidoglycans. the genes for endopeptidase (end) and endopeptidase resistance (epr) reside on plasmid pack1. an 8.4-kb fragment containing end was cloned into shuttle vector pl150 and was then introduced into staphylococcus aureus rn4220. the recombinant s. aureus cells produced endopeptidase and wer ...19957747966
the lysostaphin endopeptidase resistance gene (epr) specifies modification of peptidoglycan cross bridges in staphylococcus simulans and staphylococcus aureus.volume 61, no. 4, p. 1478, table 2, column 4: the diameters (in milliliters) of the zones of inhibition for 5-(mu)g methicillin disks given (from top to bottom), "116," "72," "107," and "32," should read "33.5," "22.6," "34.2," and "21.0," respectively. [this corrects the article on p. 1475 in vol. 61.].199516535087
cross-linked peptidoglycan mediates lysostaphin binding to the cell wall envelope of staphylococcus aureus.staphylococcus simulans bv. staphylolyticus secretes lysostaphin, a bacteriocin that cleaves pentaglycine cross bridges in the cell wall of staphylococcus aureus. the c-terminal cell wall-targeting domain (cwt) of lysostaphin is required for selective binding of this bacteriocin to s. aureus cells; however, the molecular target for this was unknown. we used purified green fluorescent protein fused to cwt (gfp-cwt) to reveal species-specific association of the reporter with staphylococci. gfp-cwt ...200616547033
peptidoglycan hydrolase fusions maintain their parental specificities.the increased incidence of bacterial antibiotic resistance has led to a renewed search for novel antimicrobials. avoiding the use of broad-range antimicrobials through the use of specific peptidoglycan hydrolases (endolysins) might reduce the incidence of antibiotic-resistant pathogens worldwide. staphylococcus aureus and streptococcus agalactiae are human pathogens and also cause mastitis in dairy cattle. the ultimate goal of this work is to create transgenic cattle that are resistant to mastit ...200616598006
a polypeptide antigen from a strain of staphylococcus simulans. 2. antigenic and biological properties.a purified polypeptide antigen from staphylococcus simulans ccm 2705 produced one precipitation line by double diffusion in agar with rabbit antiserum against homologous whole bacteria. the purified antigen did not induce antibody production in rabbits. however, when the antigen was complexed with methylated bovine serum albumin, antibodies with specificity against the polypeptide were produced. the antigen did not sensitize normal or tanned erythrocytes for agglutination in antiserum. the polyp ...19817315345
bilophila wadsworthiain brain abscess: case report.a case of a patient with a 20-year history of chronic otitis media complicated by cholesteatoma and brain abscess is described. a ct scan with contrast material showed three abscess cavities in the right cerebellar hemisphere. a culture from a specimen of the cholesteatoma yielded a significant amount of growth of bilophila wadsworthia, bacteroides fragilis and prevotella oris and a moderate growth of alpha-streptococci and staphylococcus simulans. from the pus of the brain abscess we also isola ...199716887572
immunochemical analysis of the teichoic acid from staphylococcus simulans.the wall teichoic acid of staphylococcus simulans has been characterized as a glycerol phosphate polymer with glycosidically linked n-acetylglucosamine. susceptibility to beta-n-acetylglucosaminidase and serological similarity to poly c beta from staphylococcus saprophyticus, showed that the amino sugar is in the beta-configuration.19807004077
choriogonadotropin-like antigen in a strain of streptococcus faecalis and a strain of staphylococcus simulans: detection, identification, and characterization.the presence of choriogonadotropin- and alpha-subunit-like materials in two species of bacteria identified as staphylococcus simulans and streptococcus faecalis have been demonstrated by the indirect fluorescein-labeled and the indirect peroxidase-labeled immunocytochemical techniques, utilizing antiserum for human choriogonadotropin, for its alpha and beta-subunits and the bera-subunit cooh-terminal peptide. the bacteria were originally isolated from the urine of two patients with advanced form ...19816783540
lysis of staphylococcal mastitis pathogens by bacteriophage phi11 endolysin.the staphylococcus aureus bacteriophage phi11 endolysin has two peptidoglycan hydrolase domains (endopeptidase and amidase) and an sh3b cell wall-binding domain. in turbidity reduction assays, the purified protein can lyse untreated staphylococcal mastitis pathogens, staphylococcus aureus and coagulase-negative staphylococci (staphylococcus chronogenes, staphylococcus epidermidis, staphylococcus hyicus, staphylococcus simulans, staphylococcus warneri and staphylococcus xylosus), making it a stro ...200617054440
is21-558 insertion sequences are involved in the mobility of the multiresistance gene cfr.during a study of florfenicol-resistant porcine staphylococci from denmark, the genes cfr and fexa were detected in the chromosomal dna or on plasmids of staphylococcus hyicus, staphylococcus warneri, and staphylococcus simulans. a novel variant of the phenicol resistance transposon tn558 was detected on the ca. 43-kb plasmid pscfs6 in s. warneri and s. simulans isolates. sequence analysis of a 22,010-bp segment revealed that the new tn558 variant harbored an additional resistance gene region in ...200717145796
[sensitivity to various antibiotics of coagulase-negative staphylococci isolated from samples of milk from dutch dairy cattle].during recent years the prevalence of coagulase-negative staphylococci in milk samples from dutch dairy cows has increased. in 1999 16.2% of the bacteria isolated from milk collected from cows with subclinical mastitis were coagulase-negative staphylococci. in 2004 this proportion was 42.2%. the proportion of coagulase-negative staphylococci of the bacteria isolated from milk samples from cows with clinical mastitis was 7.3% in 1999 and 14.1% in 2004. in this study, the susceptibility of 108 coa ...200717436810
subclinical mastitis changes the patterns of maternal-offspring behaviour in dairy sheep.subclinical mastitis was induced by inoculating one mammary gland in dairy ewes (n=8) with a staphylococcus simulans isolate; control ewes (n=4) were included. the milk yield of inoculated glands decreased (p<0.001), the california mastitis test (cmt) score increased and the organism could be recovered from the inoculated glands. with time, there was significantly increased frequency of "hindering sucking" (p=0.016) and "head up posture" (p<0.001) in the control ewes. infected ewes had a signifi ...200817451978
antibiotic resistance patterns of coagulase-negative staphylococcus strains isolated from blood cultures of septicemic patients in turkey.the aim of this study is to determine antibiotic resistance patterns and slime production characteristics of coagulase-negative staphylococci (cons) caused nosocomial bacteremia. a total of 200 cons strains were isolated from blood samples of patients with true bacteremia who were hospitalized in intensive care units and in other departments of istanbul university cerrahpasa medical hospital between 1999 and 2006. among 200 cons isolates, staphylococcus epidermidis was the most prevalent species ...200917475456
clinical, bacteriological, cytological and pathological features of teat disorders in ewes.we describe the features of 13 cases of teat disorders in ewes, as follows: lengthy teats (one case), papilloma infection (two), staphylococcal dermatitis (one), nodules and abscesses (two), teat bites (three), stenosis (one), orf (two), compressed teat (one). mannheimia haemolytica, staphylococcus epidermidis, staphylococcus simulans or staphylococcus aureus were isolated from samples. sub-epithelial leucocytic infiltration at the teat duct-teat cistern border, but not lymphoid nodules, was the ...200717493169
involvement of gly 311 residue on substrate discrimination, ph and temperature dependency of recombinant staphylococcus xylosus lipase: a study with emulsified substrate.the importance of microbial lipases like the staphylococcal ones, results not only from their significance implication in the bacterial lipid metabolism but also their involvement in some pathogenic processes. the aminoacid sequence of the mature staphylococcus xylosus lipase (sxl) shows 99.7% identity with the mature aminoacid sequence of staphylococcus simulans lipase (ssl) with one substitution of gly311 by val. in spite of this high homology, the two lipases present significant differences i ...200717521919
different staphylococcal species contain various numbers of penicillin-binding proteins ranging from four (staphylococcus aureus) to only one (staphylococcus hyicus).the penicillin-binding proteins of a total of 25 staphylococcal strains belonging to five different species were analyzed. all strains of the same species showed an identical penicillin-binding protein pattern which clearly differed from that of strains of the other species. staphylococcus aureus, staphylococcus intermedius, staphylococcus simulans, and the dolphin strains were found to contain two to four penicillin-binding proteins. strains of staphylococcus hyicus exhibited only one penicilli ...19854019416
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