| subarachnoid space of the cns, nasal mucosa, and lymphatic system. | we have briefly reviewed the literature pertaining to the movement of tracer molecules and infectious organisms within the olfactory nerve. there is a body of evidence indicating that tracers placed in the csf will quickly move via the olfactory nerve to the nasal mucosa and then to the cervical lymph nodes. organic and inorganic tracer materials and organisms as diverse as viruses, a bacillus, and an amoeba, when placed in the nasal cavity, have been shown to move from the nasal mucosa via the ... | 1979 | 85446 |
| relationship of intracellular 3',5'-adenosine monophosphate accumulation to parathyroid hormone release from dispersed bovine parathyroid cells. | | 1978 | 85548 |
| study on dissimilatory reduction of sulphates. | a new strain of sulphate reducing bacteria was isolated from swampy forest soil. after 120 h reduction, sulphate conversion attained 100%, the molar ratio of the consumed lactate and reduced sulphate amounted to 2:1. this confirms the reduction mechanism proposed by senez (1951). | 1979 | 87119 |
| similarity of casein- and endotoxin-induced, myeloma- associated and aged sjl/j amyloid in various strains of mice. | amyloidosis was induced in a number of strains of mice by repeated injections of casein and endotoxin. spontaneous amyloid was obtained from balb/c mice bearing a myeloma tumor (igg2a producing mopc 173 tumor) and from aged sjl/j mice. both the induced and spontaneous forms were similar in their size, immunological reactivity, peptide maps and in the susceptibility of histological sections to oxidizing agents with or without trypsin digestion. since case-induced murine amyloid resembles the noni ... | 1979 | 87374 |
| immune suppression in vivo with antigen-modified syngeneic cells. ii. t cell-mediated nonresponsiveness to fowl gamma-globulin. | intravenous administration of syngeneic spleen cells coupled with the palmitoyl derivative of fowl gammma-globulin (p-f gamma g) results in a profound state of f gamma g-specific tolerance in c57bl/6 mice. administration of p-f gamma g coupled syngeneic cells specifically reduces both the primary and secondary hapten and carrier-specific pfc responses to tnp-f gamma g. since the haptenic response is affected, the tolerance functions at the level of the f gamma g-specific helper t cell. as few as ... | 1979 | 87447 |
| changes in seizure susceptibility, sleep time and sleep spindles following thalamic and cerebellar lesions. | the present experiment attempted to clarify conflicting evidence on the relationship of sleep spindles to seizure activation. seizure thresholds were calculated in minutes post-injection following ip administration of the convulsant drug monomethylhydrazine (mmh) to cats with lesions intended to alter the occurrence of spontaneous 12-15 c/sec sleep spindles recorded from sensorimotor cortex. twelve cats with bilateral cortical and subcortical recording electrodes were divided into 3 groups recei ... | 1979 | 88323 |
| passive transfer in diabetes mellitus. | | 1979 | 88629 |
| [immunosuppression (author's transl)]. | among different procedures of immunosuppression in man medical treatment with cytotoxic drugs has become the most important. biological methods are still under development but promise to gain in importance since they are more specific and have fewer side effects. the clinical effect of immunosuppressive therapy in organ transplantation is undisputed. its effects has been proven in some but not all immuno-inflammatory diseases. before immunosuppressive therapy is started it must be known whether ... | 1979 | 88671 |
| the pathophysiology of asthma. | because postmortem studies of humans provide little information on the initial pathophysiologic events in asthma, animal models have been developed. recently the ascaris-allergic rhesus monkey has provided an opportunity to examine the onset of pathophysiologic changes following challenge and to correlate them with airway structure. these studies have suggested that the initial interaction between antigen and mast cells may occur in the bronchial lumen or in the epithelium superficial to the tig ... | 1979 | 89006 |
| mac-1: a macrophage differentiation antigen identified by monoclonal antibody. | | 1979 | 89034 |
| descending tracts of the lateral columns of the rat spinal cord: a study using the horseradish peroxidase and silver impregnation techniques. | the location of the cells of origin and the projection areas of descending fibre tracts of the spinal cord lateral columns were examined in rats. unilateral micro-transections of subpopulations of lateral column fibres, at c2 or t10, with subsequent application of horseradish peroxidase to the severed axons, allowed identification, by retrograde labelling, of those cell groups projecting to the spinal cord through the lateral columns. additionally, the pattern of fibre and preterminal degenerati ... | 1979 | 89111 |
| simulation and prevention of retrovirus--specific reactions by mycoplasmas. | from human mycosis fungoides tumor-derived cell lines, mycoplasma hyorhinis was isolated. this mycoplasma shared the following characteristics with retroviruses: uptake of 3h-uridine, but not of 3h-thymidine in cell culture; banding at 1.16 g/ml sucrose density and partial shift to retrovirus core density position (approximately equal to 1.24 g/ml) after detergent treatment; incorporation of 3h-tmp into high molecular weight material in standard reverse transcriptase assays with the template-pri ... | 1979 | 89623 |
| electron microscopic study of the rat cerebral cortex, impregnated by the golgi method. | | 1978 | 89643 |
| the development of our present views on the molecular aspects of human blood group specificity. | | 1979 | 89773 |
| antibodies in histoplasmosis detected by use of yeast and mycelial antigens in immunodiffusion and electroimmunodiffusion. | | 1979 | 89825 |
| serum concentration of human alpha 2 hs glycoprotein during the inflammatory process: evidence that alpha 2 hs glycoprotein is a negative acute-phase reactant. | a nonspecific opsonin function has been ascribed to human alpha 2 hs glycoprotein. its serum level has been shown to be decreased in trauma patients. recent studies from this laboratory revealed a heterogeneity among the products obtained in the course of the preparation of the protein. to date, no definitive agreement existed with regard to a molecular homogeneous entity of alpha 2 hs glycoprotein (ba-alpha 2 glycoproteins). the purpose of the current work was to study the variations in serum l ... | 1979 | 90057 |
| adsorption, penetration, and uncoating of murine leukemia virus studied by using its reverse transcriptase. | a procedure using the virus-associated reverse transcriptase was developed for following the kinetics of adsorption, penetration, and uncoating of murine leukemia virus. viral adsorption to cell membrane was determined by assaying this enzyme activity in isolated debris of mechanically disrupted cells after infection with murine leukemia virus in the presence of actinomycin d. at 37 degrees c, viral adsorption proceeded at a high initial rate, but after 5 min of incubation with the virus, it gra ... | 1979 | 90159 |
| [improving the effectiveness of virological studies in influenza]. | | 1979 | 90208 |
| [detection of humoral antibodies to species-specific and group ornithosis antigens in the dynamics of the infection]. | the time course of antibody production to group- and species-specific antigens of ornithosis agent was studied by complement fixation (cft) and hemagglutination-inhibition (hi) tests. in rabbits after a single intravenous inoculation of the ornithosis agent, antibody to the homologous species-specific antigen appeared in the peripheral blood 3--5 days after inoculation and reached maximum levels during the 1-st week, and to the group-specific antigen could be detected 3--5 days after inoculation ... | 1979 | 90431 |
| comparison of human alkaline phosphatase isoenzymes. structural evidence for three protein classes. | the structural relationships among human alkaline phosphatase isoenzymes from placenta, bone, kidney, liver and intestine were investigated by using three criteria. 1. immunochemical characterization by using monospecific antisera prepared against either the placental isoenzyme or the liver isoenzyme distinguishes two antigenic groups: bone, kidney and liver isoenzymes cross-react with anti-(liver isoenzyme) serum, and the intestinal and placental isoenzymes cross-react with the anti-(placental ... | 1979 | 90505 |
| murine autoimmune hemolytic anemia induced via xenogeneic erythrocyte immunization. ii. antigenic specificity and clonal heterogeneity of the autoantibodies and their cellular correlates. | | 1979 | 90574 |
| the effect of bleomycin on rat bone marrow cells. | experiments were carried out to determine the cytogenetic effects of the antibiotic bleomycin (blm) in rats using the bone marrow system. a total of eighteen male and eighteen female sprague-dawley rats were injected with varying concentration of blm, over varying periods of time. the results revealed that at low concentrations blm showed no alteration in the ploidy or the morphology of rat chromosomes. this however, was not the case when the dose or administration period was increased. | 1978 | 90585 |
| myosin isoenzyme redistribution in chronic heart overload. | since the first observation by spann et al., it has become clear that in cardiac hypertrophy induced by a mechanical overloading, the velocity of shortening of the cardiac muscle (vmax) is reduced (see ref. 2 for review). most authors agree that this mechanical alteration is accompanied by a decrease in the ca2+-dependent atpase activity of myosin (see ref. 3 for review). the molecular basis of such changes was unknown because the structural modifications of the myosin molecule were ill-defined. ... | 1979 | 91973 |
| differences in the susceptibility of mhc and non-mhc mixed lymphocyte reactions to suppression by murine amnionic fluid and its components. | the ability of mouse amniotic fluid (maf), alpha-foetoprotein (afp) and maf depleted of afp (maf - afp) to suppress primary one-way mlr's was investigated. it was found that maf, afp and maf - afp were all suppressive of mlr's specific for mhc, k, d or i + s determinants. suppression was observed when either lymph node or spleen cells were used as the responder cells. nylon wool column passage of these cells did not significantly affect the immunosuppressive action of these substances. in contra ... | 1979 | 93135 |
| cocultivation as a tool for the detection of oncoviruses in childhood leukemia. | | 1979 | 94580 |
| probing the program of gene expression utilized in early development. | somatic cell nuclei injected into amphibian oocytes enlarge, exchange protein with the surrounding cytoplasm, and actively synthesize rna for weeks. the message activity of the rnas synthesized within the oocytes can be detected as new proteins made a few days after the injection of somatic nucleic. the oocyte cytoplasm seems to reprogram the injected nuclei, allowing the expression only of those genes which are normally active in oocytes (de robertis and gurdon, 1977). genes which are unexpress ... | 1979 | 95388 |
| plasma amine oxidase activity in 136 normal rhesus monkeys: relationships between enzyme activity and age, sex and genetic factors. | normative data on plasma amine oxidase activity were obtained for four primate species, m. mulatta, m. arctoides, c. aethiops and h. sapiens. significant between-species differences in activity were observed. in a large sample of m. mulatta, no significant relationship between age and plasma amine oxidase activity was observed, but adult males had significantly higher enzyme activities than adult females. | 1977 | 96261 |
| [some aspects relating to the aflatoxin generation during the self-heating of cereals]. | in a stored batch of grain which was already affected by mould-formation tests were carried out with the known aflatoxin producer aspergillus flavus. a significantly lower aflatoxin production ensued if the mould growth was not connected with self-heating of the stored product. however, in conformity with increasing mould formation the germinating power was adversely affected and the significant signs (fatty acid number, reductive and none-reductive sugars) were influenced in the grain, irreleve ... | 1978 | 98933 |
| [a case of bacterial endocarditis due to neisseria mucosa (author's transl)]. | | 1978 | 101614 |
| [volatile fatty acids in gastrointestinal diseases in young infants]. | | 1978 | 101958 |
| clinical aspects on 64 cases of juvenile and adult listeriosis in sweden. | in 1958-74 altogether 64 cases of bacteriologically verified infections of listeria monocytogenes were diagnosed in sweden in children, aged more than 27 days, and in adults. immunosuppression predisposed to the disease. thus, many patients had co-existing disorders, such as leukemia and alcoholism. sixteen patients had been treated with corticosteroids, which were combined with cytostatic drugs in nine. meningoencephalitis was diagnosed in 52 patients and was fatal in 16. the clinical symptoms ... | 1978 | 104552 |
| effect of ultrasound on testicular electrolytes (sodium and potassium). | the testes of 50 rats were placed in a cup filled with water and received 1 w/cm2 of ultrasound for 15 min. fluid was collected from the seminiferous tubules and rete testis of the treated and control groups at 1, 8, 12, and 24 hr intervals. ultrasound increased the sodium concentration in the fluid of the seminiferous tubules, decreased the sodium concentration in the fluid of the rete testis, increased the potassium concentration in the fluid of the rete testis, and decreased the potassium con ... | 1978 | 104674 |
| assessment of the efficacy of interventions to limit ischemic injury by direct measurement of intramural carbon dioxide tension after coronary artery occlusion in the dog. | although numerous interventions have been shown to exert a salutary effect on the ischemic myocardium, the severity of ischemia generally has been measured by indirect techniques. in the present investigation the effect of ischemia on intramural carbon dioxide tension (pmco(2)) was measured directly in the open-chest, anesthetized dog with a mass spectrometer during repetitive 10-min coronary artery occlusions separated by 45-min periods of reflow; simultaneously, regional myocardial blood flow ... | 1979 | 105016 |
| wits versus genes: the continuing battle against infection. | | 1979 | 105149 |
| cell-mediated and humoral immune responses to cartilage antigenic components. | cell-mediated immunity and antibody production to cartilage antigens were studied in rabbits. from day 3 of immunization an inhibition of leucocyte migration was observed in animals immunized with collagen-free fractions of bovine nasal or human rib cartilage. delayed cutaneous hypersensitivity was elicited between 5 and 6 days and the circulating antibodies were demonstrated by passive haemagglutination on day 9. no significant correlation was observed between the antibody production and the ce ... | 1979 | 105405 |
| shedding of peripheral cytoplasm - a mechanism of liver cell atrophy in human amyloidosis. | a liver biopsy specimen from a case of primary amyloidosis was investigated by electron microscopy. the cytoplasmic periphery of the hepatocytes showed degenerativechanges which are interpreted as indicating shedding of peripheral parts of the cytoplasm. two main variants of this process could be discerned: 1) protrusion and sequestration of hernia-like blebs of cytoplasm, and 2) shedding of vesicles derived from degenerated endoplasmic reticulum. in the latter case transient defects of the plas ... | 1979 | 105463 |
| peri-oral reflexes in the conscious monkey (macaca mulatta). | | 1978 | 105692 |
| multiple sclerosis: immunochemical studies on the demyelinating serum factor. | sera from patients with multiple sclerosis (ms) frequently produce demyelination of central nervous system tissue cultures. the nature of the factors responsible for demyelination is not as yet clearly established. however, several authors previously reported, in in vivo and in vitro models, demyelinating activity in igg fractions isolated from sera and cerebrospinal fluid of ms patients14,42,48. we found the demyelinating activity of ms sera to be extremely labile to conventional biochemical tr ... | 1979 | 105780 |
| thyroxine inactivation by starvation in cultured hepatocarcinoma cells, formation of reverse triiodothyronine. | the effect of starvation on thyroid hormone metabolism was studied in monkey hepatocarcinoma monolayer cultures. nonphenolic ring monodeiodination of thyroxine, 3, 5, 3'-triiodothyronine and 3, 3'-diiodothyronine was accelerated. since phenolic ring deiodination of 3, 3',5'-triiodothyronine (reverse t3) was unaffected, this metabolite accumulated in the medium during thyroxine metabolism. this suggests that increased serum reverse t3 in malnourished humans may be caused by enhanced deiodination ... | 1978 | 105981 |
| primary structure of a human iga1 immunoglobulin. iv. streptococcal iga1 protease, digestion, fab and fc fragments, and the complete amino acid sequence of the alpha 1 heavy chain. | in order to establish the complete amino acid sequence of the human iga alpha1 chain bur, iga1 protease from streptococcus sanguis was employed to generate fabalpha and fcalpha fragments in the final stage of this investigation. cyanogen bromide cleavage of the fabalpha fragment followed by reduction and aminoethylation produced the fd' fragment (residues 84 to 227); this contains part of the variable region (vr), the whole first constant domain (calpha1), and part of the hinge region of this he ... | 1979 | 107164 |
| electron microscopical study on gamogony of sarcocystis suihominis in human tissue cultures. | sexual stages and oocysts of sarcocystis suihominis were developed in human tissue cultures and studied with the electron microscope. this development was extremely rapid, being completed about 18--22 h post infection and there were no preceding schizogonic processes, thus confirming the earlier observations that schizogony is obligatorily restricted to the intermediate host in the sarcosporidian life cycle. micro- and macrogamonts could be distinguished about 12 h post infection and were situat ... | 1979 | 107669 |
| growth and food intake of prematurely weaned rats: effect of cortisone and thyroxine injection during the suckling period. | | 1979 | 108369 |
| effects of morphine, diazepam and chlorpromazine on discrimination of electric shock. | the effects of morphine, diazepam and chlorpromazine were examined on the discrimination of electric shock in squirrel monkeys. two discrete responses measured discrimination of the presence or absence of shock at two different intensities, either at 0.35 ma or at an intensity determined to be close to each monkey's threshold (0.05 or 0.15 ma). the percentage of responses which were correct in the presence and the absence of shock were determined separately. time to respond to the presence or th ... | 1979 | 108386 |
| tuberculosis of the mandible presenting as 'lumpy jaw'. | | 1979 | 108413 |
| identification of 15-keto-9, 11-peroxidoprosta-5, 13- dienoic acid as a hematin-catalyzed decomposition product of 15-hydroperoxy-9, 11-peroxidoprosta-5, 13-dienoic acid. | a labile prostaglandin was isolated as one of the products generated from [1-14c] eicosatetraenoic acid incubated with sheep vesicular gland microsomes. the eicosatetraenoic acid metabolite amounted to ca. 16% of the total radiolabeled products. formation of this new prostaglandin was prevented when heat-denatured microsomes were employed or when incubation mixtures were supplemented with indomethacin or phenol. however, incubation of prostaglandin g2 (pgg2) with hematin in the presence or absen ... | 1979 | 108495 |
| ["in vitro" activity of a new cephalosporin (cgp-7174-e or cefsulodin) against "pseudomonas aeruginosa" (author's transl)]. | the minimal inhibitory concentration (mic) of four betalactamins (carbenicillin, ticarcillin, piperacillin and (cgp-7174-e) against 109 clinical isolates of pseudomonas aeruginosa was determined by an agar dilution method. cgp-7174-e was the most active (mic 3.4 micrograms/ml), followed in descending order by piperacillin (mic 4.9 micrograms/ml), ticarcillin (mic 22 micrograms/ml) and carbenicillin (mic 36 microgram/ml). high correlation was found between the mic of these betalactamins (r greate ... | 1979 | 120122 |
| transplacental induction of neurogenic tumors in bd ix rats by intragastric administration of ethylnitrosourea precursors. | the concurrent daily intragastric administration of ethylurea at two dose levels (50 mg/kg and 100 mg/kg bodyweight) together with one dose level of sodium nitrite (50 mg/kg bodyweight) by a stomach tube to pregnant bd ix rats from day 15 to day 22 of gestation resulted in the induction of neurogenic tumors in all offspring. since both enu-precursors alone do not produce neurogenic tumors, these results are evidence of enu formation from its precursors under the influence of gastric juice. diffe ... | 1975 | 127444 |
| clinical trial with clotrimazole cream (bay b 5097) in dermatophytosis and onychomycosis. | locally applied clotrimazole (canesten) proved to be effective in the treatment of dermatophytosis. it was also effective but to a lesser degree in cases of onychomycosis mostly due to candida albicans. the drug was well tolerated. | 1975 | 128698 |
| cytochemical studies on sarcolemma: na+, k+-adenosine triphosphatase and adenylate cyclase. | two highly lead-sensitive atpases, na+,k+-atpase and adenylate cyclase, can be demonstrated cytochemically by the lead precipitation technique in briefly prefixed tissue, provided that the free pb2+ concentration in the incubation medium is kept below 0.1 mm by a heavy metal chelator. under conditions suitable for na+,k+-atpase activity precipitation of final reaction product (lead phosphate) at the sarcolemma of cardiac muscle is abolished by 0.1-1mm ouabain. in contrast, reaction product depos ... | 1976 | 130656 |
| increased plasma prolactin levels induced in rats by d-fenfluramine: relation to central serotonergic stimulation. | d-fenfluramine (7.5 and 10 mg/kg i.p.) and quipazine (10 and 20 mg/kg i.p.) increased plasma prolactin levels in male rats. metergoline (3 mg/kg p.o.) or p-chlorophenylalanine (100 mg/kg x 3, orally) pretreatment markedly blocked the prolactin-releasing effect of both d-fenfluramine and quipazine. this result suggests that the effect of these drugs on prolactin secretion could be mediated through a serotonergic mechanism. brain serotonin may thus exert a stimulatory role on prolactin secretion i ... | 1978 | 149011 |
| [changes in atpase activity of dog kidney nuclear membrane under the effect of polyenic antibiotics]. | effect of amphotericin b and nistatin on atpase activity of dog kidney nuclear membranes is studied in vivo and in vitro. long-term intravenous injections of the antibiotics do not change the atpase activity of kidney nuclear membranes. however, short-term injections of polyenic antibiotics have some effect on atpase activity on nuclear membranes: amphotericin b, considerably activates the enzyme . in vitro incubation of isolated dog kidney nuclei with amphotericin and nistatin at concentrations ... | 1978 | 149566 |
| effect of d-amphetamine on tryptophan and other aromatic amino acids in brain. | the investigation examined the mechanism of the increase in brain tryptophan concentration of rats treated with d-amphetamine. certain well recognised influences upon brain tryptophan have been excluded as responsible. thus, the effect is not associated with changes in the plasma concentrations of nefa or free tryptophan. it is probably not due to a tryptophan-specific mechanism, because amphetamine increases the ratio of brain/plasma concentrations not only of tryptophan but also of tyrosine an ... | 1978 | 149670 |
| the presence of two (na+ + k+)-atpase inhibitors in equine muscle atp: vanadate nad a dithioerythritol-dependent inhibitor. | a potent inhibitor of (na+ + k+)-atpase activity was purified from sigma equine muscle atp by cation- and anion-exchange chromatography. the isolated inhibitor was identified by atomic absorption spectroscopy and proton resonance spectroscopy to be an inorganic vanadate. the isolated vanadate and a solution of v2o5 inhibit sarcolemma (na+ + k+)-atpase with an i50 of 1 micrometer in the presence of 1 mm ethyleneglycol-bis-(beta-aminoethylether)-n,n'-tetraacetic acid (egta), 145 mm nacl, 6mm mgcl2 ... | 1978 | 150289 |
| [effect of beryllium salts on the activity of sarcoplasmic reticulum ca2+,mg2+-dependent atp-ase]. | in the presence of mg2+ and ca2+ ions beryllium compounds inhibit the ca2+, mg2+-dependent atpase activity and the transport of ca2+ in the sarcoplasmatic reticulum vesicles. the inhibition is reversible and concurrent with respect to the mg2+ ions. in the absence of the mg2+ ions an addition of beryllium compounds stimulates the atpase activity, the dependence of the degree of its stimulation on the beryllium compounds concentration is characterized by a curve with a maximum. on the membranous ... | 1979 | 154413 |
| computerized axial tomography in syringomyelia. | the acta-scanner, a device for computerized axial tomography, permits cross-sectional radiographic study of the entire human body, including the spine. in the acta-scan, the spinal cord appears as a roundish formation surrounded by the less dense subarachnoidal cerebrospinal fluid. the spines of 18 patients with verified (nine cases) or suspected (nine cases) syringomyelia were studied by acta-scanning. in seven of the verified and in four of the nonverified cases, some evidence of cord cavitati ... | 1975 | 162787 |
| transplantation of hepatomas induced in the avian liver by mc29 leukosis virus. | a hepatomatous growth derived from primary liver tumors induced in chickens by i.v. inoculation with mc29 leukosis virus has been established and maintained in the avian host. hepatoma tissue transplanted into the abdominal cavity in a total of 278 chicks in 35 experiments yielded tumors in 222 animals (80%). the i.m. implantation in 69 birds in 7 experiments resulted in growth in 67 chicks (97%). tumor tissue introduced inadvertently into the s.c. tissue likewise grew very rapidly. histological ... | 1975 | 162857 |
| swine buffy coat culture: an aid to the laboratory diagnosis of hog cholera. | a 2-step technique for the isolation of hog cholera (hc) virus consisting of an initial culture on buffy coat (bc) cultures and subinoculation to a pig kidney cell line (pk-15) was described. by this technique, hc virus was confirmed in specimens from 65 herds in which the conventional cell culture isolation technique failed. the herds were located in 20 states and puerto rico. specimens from 29 of the 65 herds were inoculated into specific-pathogen-free (spf) pigs. hog cholera virus was recover ... | 1975 | 163064 |
| a guanosine 3':5'-monophosphate-sensitive nuclease from bacillus brevis. | in toluene-treated cells of bacillus brevis, newly synthesized rna is rapidly degraded in a reaction that is inhibited by cyclic guanosine 3':5'-monophosphate (cgmp) and by 1,10-phenanthroline. this appears to be due to a ribonuclease found in cell-free extracts of b. brevis which is inhibited by cgmp and related compounds as well as by 1,10-phenanthroline. the cgmp-sensitive nuclease hydrolyzes synthetic polynucleotides, yielding nucleoside 5'-monophosphates as the sole products, even during th ... | 1975 | 163232 |
| lipid composition of bovine serum lipoproteins. | the four classes of lipoproteins (one of very low density, two of low density, and one of high density) were isolated from the serum of a lactating friesian cow. the proportions of protein and of the different lipid classes were determined in each lipoprotein. triglycerides predominated in the very low density lipoprotein, and cholesteryl esters and phospholipids in the others. the triglycerides of the very low density lipoprotein were richer in oleic acid than were those of the low density lipo ... | 1975 | 163266 |
| mechanism of the suppressive effect of interferon on antibody synthesis in vivo. | mouse interferon preparations significantly suppress the in vivo antibody response to sheep red blood cells (srbc), a thymus-dependent antigen, and to salmonella typhimurium lipopolysaccharide (lps), a thymus-independent antigen. it is also possible to effect the late responses of antigen sensitive "memory" cells observed during secondary immunization by administration of interferon prior to primary immunization. the immunosuppressive activity of interferon was time- and dose-dependent. maximum ... | 1975 | 163863 |
| correlation of estrogen-induced uterine eosinophilia with other parameters of estrogen stimulation, produced with estradiol-17beta and estriol. | estradiol-17beta is a stronger estrogen than estriol for the genomic response of estrogens. estriol is a stronger estrogen than estradiol-17beta for the estrogen-induced uterine eosinophilia and the 6 h increase in the uterine wet weight. | 1975 | 169148 |
| [in vitro evaluation of antiviral inhibitors by means of a turbidity method (author's transl)]. | the paper presents a photometric method for the determination of virus-induced alterations (cytopathogenic effects) of cells based on nephelometric analysis. these were taken at varying time intervals after infection and with different multiplicity of infection. it was demonstrated that suppression of virus-induced cpe by the known inhibitors could also be followed by turbidity measurements. the in vitro experiments were carried out in the 2 following virus-cell-systems: pseudorabies virus in ch ... | 1975 | 171906 |
| studies of genetic transmission of mammary tumour virus by c3hf mice. | by radioimmunoassay (ria) mammary tumour virus (mtv) antigens were detected in individual milk samples of c3hf mice, (female balb/c x male c3hf)f1 mice and (female c3hf x male balb/c)f1 mice; milk samples of balb/c mice were negative. in the segregating backcross i population, female balb/c x male (female balb/c x male c3hf) viral antigens were found in the milk of 93 out of 169 mice (55%). in the bc ii population (daughters of bc i mothers and balb/c fathers) two groups were distinguished. in t ... | 1975 | 172460 |
| the effect of cyclic amp and prostaglandins on the fibrinolytic activity of mouse neuroblastoma cells. | | 1976 | 174561 |
| mapping and gene conversion studies with the structural gene for iso-1-cytochrome c in yeast. | we have investigated the order of the four genes cyc1, rad7, sup4, and cdc8 which form a tightly linked cluster on the right arm of chromosome x in the yeast saccharomyces cerevisiae. crossing over and coconversion data from tetrad analysis established the gene order to be centromere-cyc1-rad7-sup4. also cdc8 appeared to be distal to sup4 on the basis of crossovers that were associated with conversion of sup4. the frequencies of recombination and the occurrence of coconversions suggest that thes ... | 1975 | 174970 |
| epstein-barr virus-specific iga serum antibodies as an outstanding feature of nasopharyngeal carcinoma. | stimulated by a report on elevated iga levels in nasopharyngeal carcinoma (npc), we tested a total of 372 sera from patients with npc, other carcinomas of head and neck or elsewhere, burkitt's lymphoma (bl), infectious mononucleosis (im) or healthy controls. the sera were titrated in indirect immunofluorescence tests for iga antibodies to epstein-barr virus (ebv) capsid antigen (vca) and to the diffuse (d) or restricted (r) components of the ebv-induced early antigen (ea) complex. the results pr ... | 1976 | 175020 |
| testicular protein kinases. characterization of multiple forms and ontogeny. | protein phosphokinase activity from the cytosol (105,000 x g soluble fraction) of testes from sexually mature rats has been resolved be deae-cellulose chromatography in three forms of protein kinase, camp-dependent protein kinases i and ii and camp-independent protein kinase iii. adenosine 3':5'-monophosphate-binding activity (camp-binding activity) was associated with protein kinases i and ii but not with protein kinase iii. protein kinases i, ii, and iii exhibited different ph optima, cyclic n ... | 1976 | 175066 |
| androgen and estrogen receptors in brain cytosol from male, female, and testicular feminized (tfm/y hermaphrodite) mice. | specific binding of [3h] 5alpha-dihydrotestosterone (dht) and [3h] estradiol by cytoplasmic extracts from whole brain of castrated male, female, and androgen-insensitive, testicular feminized (tfm/y male-female), mice has been investigated using glycerol gradient centrifugation and charcoal assay. mouse brain cytosol contains macromolecules with the characteristics of steroid hormone receptors, binding preferentially with high-affinity androgens or estrogens. both dht- and estradiol-receptor com ... | 1976 | 179788 |
| steroid production by quartered and capsular rat adrenal gland in response to haemorrhage. | 60 min after rapid bleeding (1.5--2.0 per cent of b. w.) both aldosterone and corticosterone production rate by quartered rat adrenals were found to be elevated. however, no difference was observed in the rate of aldosterone and corticosterone production by capsular adrenals of sham operated and hypovolaemic rats. corticosterone production rate by decapsulated adrenals was much more higher after haemorrhage than in the control group. the same alterations could be observed incubating adrenal tiss ... | 1975 | 179800 |
| relationship between receptor and mammary tumour virus production after stimulation by glucocorticoid. | a short exposure of primary cultures of mouse mammary tumour cells to glucocorticoids results in at least a three-fold stimulation of mammary tumour virus (mtv) production. specific interaction of glucocorticoids with the cytoplasmic and nuclear receptors can also be demonstrated. the biological potency of various steroids to stimulate mtv is related directly to the retention of the steroid-receptor complex in the nuclei. progesterone has a high affinity for the cytoplasmic receptor, is not reta ... | 1976 | 180430 |
| topographical anatomy of human cerebral and cerebellar astrocytomas in vitro. | this study further characterizes the morphological and in vitro properties of human neoplastic cells derived from one grade iv cerebral astrocytoma and one grade iii cerebellar astrocytoma. light microscopic observations performed weekly over a four month period revealed two morphologically indistinguishable cell populations in each of the tumor cultures. scanning electron microscopy demonstrated the presence of microvilli, blegs, and ruffles on the cell surfaces of both tumors; however, these t ... | 1976 | 188206 |
| epstein-barr-virus-specific iga and igg serum antibodies in nasopharyngeal carcinoma. | the sera of 73 patients with nasopharyngeal carcinoma (npc), 28 patients with other carcinomas (oc) and 89 healthy subjects (hs) were tested for igg and iga antibodies to epstein-barr virus (ebv) viral capsid antigen (vca). the majority of the npc sera had igg titres of 160 or above, whereas the majority of the other sera had titres below 160. for iga reactivity to ebv-vca, 68 of 73 (93-2%) npc sera had titres of greater than or equal to 10. in contrast, only 6 of 28 (21-4%) oc sera and none of ... | 1976 | 188437 |
| naturally-occurring neonatal canine herpesvirus infection. | the detailed clinical and epidemiological history and the pathology of neonatal canine herpesvirus (chv) disease in a naturally infected litter of pups is reported. herpesvirus was isolated from the brain, lung, liver, spleen, kidney and intestine of one of the affected litter which died on day 10 after whelping. the isolation of virus correlated with the distinctive gross and histological changes in these organs. herpesvirus was isolated also from the anterior vagina of the bitch 18 days after ... | 1976 | 189479 |
| biochemical and immunological characterization of deoxythymidine kinase of thymidine kinaseless hela cells biochemically transformed by herpes simplex virus type. | thymidine kinase (tk) from herpes simplex virus type 1 (hsv-1) biochemically transformed hela cells, purified by affinity chromatography, has been characterized with respect to its electrophoretic mobility, molecular weight, activation energy, substrate specificity, and immunological specificity. tk purified from hsv-1-transformed hela cells has the same electrophoretic mobility as tk purified from hela cells lytically infected with hsv-1. the sedimentation velocity of purified tk from transform ... | 1977 | 193790 |
| p1, p5-bis-(5'-adenosyl)pentaphosphate: is this adenylate kinase inhibitor substrate for mitochondrial processes? | 1. p1, p5-bis-(5'-adenosyl) pentaphosphate (ap5-a) inhibits "soluble" adenylate kinase even when this enzyme is an integral part of the complete mitochondrion. the ki is 10(-5) m, i.e. about two orders of magnitude higher than the inhibitor contants determined for the purified adenylate kinase of rabbit muscle and an enzyme preparation separated from the mitochondrial intermembrane space. the weaker inhibitory effect is due to a lower accessibility of the enzyme. 2. as to be expected ap5a which ... | 1977 | 201118 |
| epstein-barr virus and cytomegalovirus antibodies in infectious mononucleosis. | a method has been evolved for the demonstration of epstein-barr virus (ebv) and cytomegalovirus (cmv) infection in 83 cases of infectious mononucleosis. serum samples were tested for ebv igm, anti-vca igg, anti-ebna, cmv igm and cmv igg antibodies. an acute-phase sample (or samples) and a convalescence sample were examined in each case, and in 44 cases an additional samples was examined 5-12 months after the illness. since the different antibodies showed characteristic differences in both titre ... | 1977 | 201153 |
| enzyme-catalyzed dna unwinding. mechanism of action of helicase iii. | | 1979 | 227887 |
| from long-leggety beasties - good lord, deliver us. | | 1979 | 260080 |
| alkylphthalides isolated from ligusticum wallichii franch and their in vitro inhibitory effect on rat uterine contraction induced by prostaglandin f2alpha. | | 1977 | 271195 |
| shunting of microspheres across the canine coronary circulation. | coronary shunting of 9 +/-1 micrometer and 25 +/- 5 micrometer radiolabeled microspheres was examined in anesthetized, open-chest dogs, whose left common coronary arteries were perfused at controlled pressures. shunting was estimated from the difference in radioactivity between perfusion line and coronary sinus blood samples during selective elevations of coronary perfusion pressure (cpp), left ventricular afterload, and inspired oxygen. a linear relationship was found between coronary shunting ... | 1979 | 434177 |
| a chronic cannula for obtaining csf from the cisterna magna of awake dogs. | we have designed a cannula system that can be chronically implanted to end above the dura of the cisterna magna of the dog. during experiments in the awake dog, a screw cap with stylet is removed from the cannula and a spinal needle inserted for the withdrawal of samples of cisternal cerebrospinal fluid (csf) or for making continuous measurements of pressure. the system can be used for repeated experiments extending over several weeks. | 1979 | 434184 |
| an xyy man. | this case report describes a young man with a 47, xyy karyotype who was convicted of arson. he suffered from a cardiac disorder which may well have been part of the xyy syndrome rather than a chance association. his abnormal karyotype was disclosed in court and used by the defence in a plea in mitigation. | 1979 | 497632 |
| interactions of folch-lees proteolipid apoprotein with planar lipid bilayers. | water-soluble folch-lees proteolipid apoprotein from bovine cns white matter induces a voltage-dependent conductance in black lipid membranes. na+ is required for the induced conductance change but the established conductance has very low ionic selectivity. the induced conductance fluctuates with a minimum amplitude of 10(-11)--10(-10) mho. the magnitude of the conductivity change is dependent on protein concentration and on the composition of lipid bilayers. at a fixed voltage the induced condu ... | 1979 | 522128 |
| [microionophoretic study of the mechanism of action of gamma-hydroxybutyrate]. | the effect of gamma-hydroxybutyric acid (ghba) on the extracellularly registered spontaneous electrical activity of nervous cells of the rabbit brain sensomotor cortex was studied using the microionophoretic technique. ghba decreased the frequency of action potential in the majority of the neurons studied. a specific gamma-aminobutyric acid (gaba) blocking agent bicuculline prevented gbha inhibitory effect. ghba is suggested to interact with central gaba-receptors. the frequency of discharges in ... | 1979 | 540145 |
| intrasellar abscess. | a case of intrasellar abscess in a 31-year-old male, with pituitary insufficiency, visual field and acuity disturbances, is reported. in the pus extracted at surgery e. coli and staphylococcus haemolyticus were identified. the patient died 20 days after the operation. necropsy revealed the fibrous capsule of an abscess in the hypophyseal region and putriform tissue at the level of the hypothalamus, with three small tumours one of which proved to be a craniopharyngioma. | 1975 | 765877 |
| [the acute vestibular paralysis (author's transl)]. | acute vestibular paralysis may not be considered as a nosologic entity but as a syndrome. symptomatology (vertigo, spontaneous and provoked vestibular nystagmus, absence of cochlear signs) shows an uniform picture. the results of the caloric test as well as the nystagmic responses induced by galvanic stimulation and the development of central vestibular compensation however indicate that the site of the lesion is not only confined to the labyrinth but may also occur at the level of the periphera ... | 1975 | 1081389 |
| morphology of the perivascular regions of the rat neural lobe in relation to hormone release. | neural lobes of rats subjected to dehydration by drinking 2% saline for four days were examined electron microscopically and compared to untreated controls. the ultrastructure of the blood vessels and the tissues surrounding them were examined and it was found that, although few exocytotic figures could be seen in either group of animals, a significantly larger (p smaller than 0.01) number os small vesicles were found in nerve endings adjacent to the perivascular space in the saline treated grou ... | 1975 | 1149110 |
| [the effect of serotonin on the release of free fatty acids from human and rat adipose tissue (author's transl)]. | | 1975 | 1156892 |
| virus infections and kidney disease. | | 1975 | 1196274 |
| changes in blood flow to bones during the hypocalcemic and hypercalcemic phases of the response to parathyroid hormone. | labeled microspheres were used to measure blood flow to the leg bones of the laying hen at 0, 3, and 30 min after iv injection of parathyroid hormone (pth) (wilson) or the synthetic 1 to 34 fragment of bovine parathyroid hormone (pth 1-34). at 3 min, which corresponds to the hypocalcemic phase of the pth response, blood flow to the combined femur, tibia, and metatarus was significantly reduced by pth (wilson) relative to 0 time and to carrier-injected controls. at 30 min, i.e., the time of maxim ... | 1976 | 1248454 |
| renaturation of bacteriophage lambda dna. determination of the optimal renaturation conditions using a single-strand-specific dnase and alkaline-sucrose-gradient assay system. | reannealed hybrid molecules of wild-type bacteriophage lambda dna were prepared in aqueous solutions of formamide at a variety of nacl concentrations at both room temperature ( 22 degrees c) and 37 degrees c. treatment of the hybrid dna molecules with the single-strand-specific nuclease s1 from aspergillus oryzae followed by alkaline sucrose gradient sedimentation was used to monitor the extent and fidelity of hybridization. the optimal renaturation conditions at room temperature were found to b ... | 1976 | 1248479 |
| detection and purification of a potential precursor protein or a prohaemolysin produced by staphylococcus haemolyticus. | the haemolytic substance h7 produced by staphylococcus haemolyticus is composed of three peptides out of 44 amino acid residues each having a structure resembling a signal peptide. the cytoplasmic fraction for the presence of a protein precursor containing this signal sequence was investigated. specific rabbit iggs to substance h7 were isolated by affinity chromatography on sepharose-4b-h7. these anti-h7 iggs recognized mainly a 51 kd protein in the cytoplasmic fraction of s. haemolyticus from 2 ... | 1992 | 1282650 |
| novel membrane proteins present in teicoplanin-resistant, vancomycin-sensitive, coagulase-negative staphylococcus spp. | clinical isolates of staphylococcus epidermidis and staphylococcus haemolyticus resistant to teicoplanin (mic 64 mg/l) and sensitive to vancomycin (mic 2 mg/l), were compared with vancomycin- and teicoplanin-sensitive isolates (mics 1 mg/l) of the same species. no apparent differences between the sensitive and resistant strains of either pair were found with respect to binding of teicoplanin to the bacteria, or to the amino acid content or degree of cross-linkage of purified peptidoglycan. the r ... | 1992 | 1289350 |
| effects of gene transfer into cultured cns neurons with a replication-defective herpes simplex virus type 1 vector. | vectors derived from herpes simplex virus type 1 (hsv-1) may provide useful tools for gene transfer to cells of the mammalian nervous system. we have studied the infection of cultured cns neurons using a vector derived from an hsv-1 mutant deleted for the major hsv-1 transcriptional regulatory protein-encoding gene, ie 3. this vector, denoted cgal delta 3, contains the e. coli lacz gene driven by the strong promotor of the human cytomegalovirus major immediate-early gene inserted into a non-codi ... | 1992 | 1312210 |
| underdiagnosis of genital herpes by current clinical and viral-isolation procedures. | the current clinical strategy for diagnosing genital herpes simplex virus (hsv) infection in women relies on clinical findings plus the selective use of viral culture. the effectiveness of this approach for identifying women with genital herpes is unknown. | 1992 | 1315930 |
| metabolic inhibition and electrical properties of type-1-like cortical astrocytes. | type-1-like cortical mouse astrocytes were studied in homogeneous cultures. membrane input resistance and membrane potential were measured during drug-induced inhibition of glycolysis (sodium fluoride), mitochondrial respiration (antimycin-a) and na+/k+ pump activity (ouabain). it was found that the electrical properties of the astrocytes recovered after a 60 min period with inhibited glycolysis or mitochondrial respiration, exhibiting only small reversible depolarizations. a 60 min period of hi ... | 1992 | 1315934 |
| differential expression of two sodium channel subtypes in human brain. | two partial human brain sodium channel cdna sequences (designated hbsc i and ii) have been cloned and mapped to chromosome 2q23-2q24 by chromosome microdissection-pcr (cmpcr). the distribution of hbsc i and ii mrna in human brain was studied by means of a novel approach based on the ligase detection reaction. these studies demonstrate that hbsc i and ii mrna is heterogeneously distributed in brain, and that the relative ratio of the two forms can vary as much as 7-fold between different regions. | 1992 | 1317301 |
| in vitro evaluation of e-4695, a new fluoro-naphthyridine. | compound e-4695, a c-7 azetidinyl fluoro-naphthyridine, was compared to six structurally related quinolones and was generally two- to four-fold more active than ciprofloxacin. e-4695 was particularly active against staphylococcus aureus (mic90 0.06 mg/l), staphylococcus haemolyticus (mic90 0.5 mg/l), klebsiella spp. (mic90 less than or equal to 0.015 mg/l), serratia marcescens (mic90 0.06 mg/l), acinetobacter spp. (mic90 less than or equal to 0.015 mg/l), pseudomonas aeruginosa (mic90 0.5 mg/l), ... | 1992 | 1327787 |
| colonization with coagulase-negative staphylococci in two neonatal units. | episodes of septicaemia due to coagulase-negative staphylococci (cns) were more frequent in a level iii than in a level ii neonatal unit in stockholm, sweden. colonization with cns during the first 2 weeks of life was investigated in 10 infants from each unit. as the use of antibiotics differed between the two units, the aim was to correlate colonization and antimicrobial resistance patterns to antibiotic usage. antimicrobial susceptibility of cns to isoxazolylpenicillins, co-trimoxazole, erythr ... | 1992 | 1363108 |
| plasmid-encoded resistance to tetracycline in staphylococcus haemolyticus. | a small 4.35 kb plasmid, designated psts4, was isolated from a multiresistant staphylococcus haemolyticus culture. it conferred resistance to tetracycline as shown by protoplast transformation. psts4 was further characterized by restriction endonuclease analyses and a preliminary restriction map constructed. it shared some structural similarities with previously described small tcr plasmids from other staphylococcal species of human and animal origin. psts4 encoded two proteins of approximately ... | 1992 | 1368787 |