| similarity of casein- and endotoxin-induced, myeloma- associated and aged sjl/j amyloid in various strains of mice. | amyloidosis was induced in a number of strains of mice by repeated injections of casein and endotoxin. spontaneous amyloid was obtained from balb/c mice bearing a myeloma tumor (igg2a producing mopc 173 tumor) and from aged sjl/j mice. both the induced and spontaneous forms were similar in their size, immunological reactivity, peptide maps and in the susceptibility of histological sections to oxidizing agents with or without trypsin digestion. since case-induced murine amyloid resembles the noni ... | 1979 | 87374 |
| oncofetal antigen: a tumor-associated fetal antigen immunogenic in man. | oncofetal antigen (ofa) has been defined with the use of human natural antibodies as a membrane antigen of human cancer cells that cross-reacts with human fetal brain tissues. the immunogen that elicits the antibody is unknown. the present study was undertaken to examine the immunogenicity of the ofa found on tumor cells. postoperative melanoma patients were immunized with ofa-positive melanoma cells. anti-ofa reactivities in the immunized sera were titrated by the immune adherence assay with th ... | 1979 | 88539 |
| experimental studies of the pathogenesis of infections due to pseudomonas aeruginosa: extracellular protease and elastase as in vivo virulence factors. | the effects on mortality of supplemental injections of protease and elastase were determined in burned mice infected with non-lethal inocula of a toxin-producing but non-proteolytic-enzyme-producing strain of pseudomonas aeruginosa. when a variety of solutions containing proteolytic enzyme were injected under these conditions, the mortality increased significantly. this did not occur when organisms other than p. aeruginosa were used. injections of the enzyme solutions alone were non-lethal. inje ... | 1979 | 89891 |
| rubella: which vaccine? | | 1979 | 90959 |
| abnormal levels of antibodies to measles-virus proteins in patient with mental retardation and seizures 24 years after measles encephalitis. | | 1979 | 91066 |
| myosin isoenzyme redistribution in chronic heart overload. | since the first observation by spann et al., it has become clear that in cardiac hypertrophy induced by a mechanical overloading, the velocity of shortening of the cardiac muscle (vmax) is reduced (see ref. 2 for review). most authors agree that this mechanical alteration is accompanied by a decrease in the ca2+-dependent atpase activity of myosin (see ref. 3 for review). the molecular basis of such changes was unknown because the structural modifications of the myosin molecule were ill-defined. ... | 1979 | 91973 |
| [heterologous transplantation of the human medulloblastoma--with particular reference to the histogenesis of medulloblastoma (author's transl)]. | | 1979 | 91984 |
| ia determinants on human t-cell subsets defined by monoclonal antibody. activation stimuli required for expression. | the nature of ia antigens which appear on human t cells after activation and the stimuli required for their expression was examined utilizing a monoclonal antibody reactive with the ia antigen framework. t cells were purified using monoclonal antibodies directed either at the entire t-cell population (okt3) or the t-cell inducer subset (okt4). by indirect immunofluorescence, it was shown that the human t-cell population contains no detectable ia+ cells in the resting state. in contrast, in exces ... | 1979 | 92523 |
| ionomycin stimulates mast cell histamine secretion by forming a lipid-soluble calcium complex. | the activation of cellular function following the direct introduction of ca2+ into the cytosol by the use of a ca2+-transporting ionophore has served to confirm the widely held idea that ca2+ has the status of a second messenger in many cell types. however, this evidence has been obtained largely from the use of a single ionophore, the antibiotic a23187; experiments with x537a, which is another ionophorous antibiotic capable of transporting ca2+ (ref. 3), have failed to show the expected charact ... | 1979 | 92764 |
| differences in the susceptibility of mhc and non-mhc mixed lymphocyte reactions to suppression by murine amnionic fluid and its components. | the ability of mouse amniotic fluid (maf), alpha-foetoprotein (afp) and maf depleted of afp (maf - afp) to suppress primary one-way mlr's was investigated. it was found that maf, afp and maf - afp were all suppressive of mlr's specific for mhc, k, d or i + s determinants. suppression was observed when either lymph node or spleen cells were used as the responder cells. nylon wool column passage of these cells did not significantly affect the immunosuppressive action of these substances. in contra ... | 1979 | 93135 |
| [changes in blood levels of noradrenaline in man following short and long term exogenous infusion]. | norepinephrine (ne) was infused in four normal men in doses of 1.5, 6.0 and 9.0 microgram/min for 60 min. the rise in plasma ne was observed only after 5 min and steady state plasma ne and infusion rates. the calculated turnover time averaged 35 sec for 1.5 microgram/min infusion and 20 sec for both 6.0 and 9.0 microgram/min infusion experiments. these differences may be explained by adjustments occurring in inactivation processes in relation to infused doses. a basal endogenous overflow rate of ... | 1979 | 93441 |
| spiroplasmas: serological grouping of strains associated with plants and insects. | spiroplasma strains from plant and arthropod hosts, and from surfaces of flowers, were classified into three serological groups (designated i, ii, and iii) based on results from growth-inhibition tests. no significant cross reactions were observed among groups. the groupings were confirmed by ring-interface precipitin and microprecipitin tests, using membrane preparations as test antigens, and by organism-deformation tests. serogroup i contained three subgroups: subgroup a (spiroplasma citri str ... | 1979 | 93504 |
| an anti-(signal peptide) antibody: purification, properties and use as a conformational probe. | | 1979 | 93537 |
| generation of specfic t-cell suppressor function induced by streptococcus mutans in monkeys and mice. | specific suppressor cells in mice and monkeys can be induced with high doses of streptococcus mutans antigen. when these cells are further cultured with a low dose of s. mutans antigen in vitro, they secrete specific suppressor factors which decrease the cooperative responses to this antigen. in view of the sensitivity of these cells to anti-thy 1 and complement, and as they are not retained on nylon-wool, these suppressor cells are t cells. the suppressor factor exerts its effect on t helper ce ... | 1979 | 93576 |
| in vivo induction of endogenous retroviruses in balb/c mouse hepatocytes by successive treatments with carbon tetrachloride and bromodeoxyuridine. | the effects of bromodeoxyuridine (brdurd) incorporation into hepatocytes of carbon tetrachloride (ccl4)-induced regenerating liver of balb/c mice on the induction of endogenous retroviruses was examined. from the nucliec acid hybridization studies, the maximum levels of hybridization were obtained for both n- and x-tropic balb/c endogenous retrovirus specific [3h]-cdnas with liver rna from animals receiving brdurd at 40 and 44 h post-ccl4 treatment, and killed on the fourth day following brdurd ... | 1979 | 93580 |
| the effect of an escherichia coli regulatory mutation on transfer rna structure. | | 1979 | 93644 |
| liposome-incorporated enzymes: studies on amylase. | alpha-amylase (ec 3.2.1.1) from hog pancreas was incorporated into artificial phosphatidylcholine and phosphatidylserine liposomes. to kinetically follow the enzyme-catalyzed hydrolysis of amylose the liposome-bound amylase was incubated in a medium containing amylose-iodine substrate. the reaction was studied at different concentrations of amylose and at different ionic strengths. activation of amylase incorporated into liposomes by chloride ions varies with the type of phospholipid utilized to ... | 1979 | 93989 |
| effect of beta-blockers on sodium and potassium content of human erythrocytes. | recently, hyperpotassemia was reported in patients treated with propranolol, but the mechanism has not yet been delineated. we have investigated the effects of various beta-adrenergic blockers and of d-propranolol on the na+ and k+ distribution intra- and extracellularly in human erythrocytes. k+ loss and na+ gain by cells was demonstrated at drug concentrations of 10(-4) m or greater. d-propranolol was more effective than l-propranolol, whereas pindolol was ineffective. practolol increased na+ ... | 1979 | 94824 |
| [role of calcium in realization of nervous control during rna synthesis in skeletal muscles]. | the effects of ca2+ on the rna polymerase activity of the nuclei isolated from normal and denervated gastrocnemius muscles of the rabbit were studied. it was shown that 18 hrs after denervation the rna synthesis in vitro, ca2+ content and the ca, mg-atpase activity of the nuclei are decreased. after addition of exogenous ca2+ the incorporation of labelled utp into the nuclei is stimulated in the denervated muscle and is inhibited in the control. electrostimulation of the denervated muscle at the ... | 1979 | 94840 |
| separation of small dna and rna oligonucleotides by high-performance anion-exchange liquid chromatography. | small oligonucleotides from dna and rna have been separated according to their base composition by high-performance anion-exchange liquid chromatography on partisil-10 sax using triethylammonium acetate buffer as the eluent. fifteen of the 16 possible deoxydinucleoside monophosphates and all 16 dinucleoside monophosphates have been separated. all pairs of sequence isomers were all resolved. the 15 commercially available deoxydinucleotides were resolved into 13 fractions. a good resolution of deo ... | 1979 | 94913 |
| mechanisms of immunity in bacterial infections. | | 1979 | 95156 |
| histochemical characteristics of the anal sacs of the cat. | the histochemistry of polysaccharides and proteins and the distribution of rna were investigated in the glands and mature secretion of the anal sacs of the cat. glycogen was extremely abundant in the epithelial lining of the lumen and efferent duct of the anal sac and also in ducts of sebaceous glands of the sacs constantly formed in the place of degenerating acini. little glycogen was found in the unchanged sebaceous acini. the apocrine glands of the anal sacs were substantially richer in glyco ... | 1979 | 95391 |
| dynamic inorganic ion-protein interactions in structural organization of dna of living cell nuclei. | staining polarization optical techniques showed differences in the structural organization of dna of chromatin in interphase nuclei and in mitotic chromosomes. the dna was non-birefringent in intact interphase cell nuclei, but birefringent in chromosomes and in isolated nuclei incubated in a physiological electrolyte solution. the birefringence of dna appears to be related to an unfolding of dna filaments induced by free cations and to the oriented binding of dye molecules to dna phosphates. we ... | 1979 | 95394 |
| fetal hemoglobin: in vivo stimulation by d-thyroxine in adult marmosets. | reactivation of fetal hemoglobin gene expressions was studied in new-world monkeys (marmosets). after a series of intraperitoneal injections of d-thyroxine, fetal hemoglobin production was increased in three experimental animals. control animals were unaffected by the injection of the solvent. because the percentage of cells containing fetal hemoglobin exceeded the total percentage of fetal hemoglobin, it was concluded that thyroid hormone influenced the synthesis of fetal hemoglobin rather than ... | 1979 | 95517 |
| [morphological and histochemical examinations of the white rat testis after long-term administration of low doses of lindan (author's transl)]. | | 1979 | 95557 |
| metabolism of bile acids. v . metabolism of ursodeoxycholic acid in rhesus monkey. | | 1977 | 97247 |
| immunohistochemical identification of lysosome in paneth cells. | | 1978 | 97792 |
| comparison of arachnoiditis produced by meglumine iocarmate and metrizamide myelography in an animal model. | myelography in monkeys was performed with either meglumine iocarmate or metrizamide. the severity of resultant arachnoiditis in each animal 12 weeks later was evaluated by repeat myelography and by histologic study of the arachnoid. three of the four animals examined with meglumine iocarmate had severe arachnoiditis; none of the four animals examined with metrizamide had as severe changes. the difference between the two groups was statistically significant (p less than .05). | 1978 | 97964 |
| esophagogastrostomic anastomosis instrument. | | 1978 | 98303 |
| anterior chamber volume determination in the rhesus monkey. | | 1978 | 98472 |
| histochemical study of labial salivary glands in sjögren's syndrome. | human labial salivary gland biopsies of patients presenting connective tissue diseases associated with sjögren's syndrome were submitted to a polysaccharide histochemistry study. the normal acinar secretion is an association of neutral polysaccharides with a sulphosialomucin. in sjögren's syndrome, there is a great reduction in the secretory activity of the acinar cells, but no qualitative change was observed. the pathogenesis of this decreased production and its importance regarding the clinica ... | 1978 | 98631 |
| decrease in pentobarbital sleeping time by trh: characterization with power spectral analysis of eeg. | | 1978 | 99751 |
| inhibition of diamine oxidase by 1,1-[(methylethanediylidene)-dinitrilo]-bis-(3-aminoguanidine) and 1,1'-[(methylethanediylidene)-dinitrilo]-diguanidine. | | 1978 | 100119 |
| experimental investigations into the etiology of cholangitis following operation for biliary atresia. | | 1978 | 101651 |
| absolute configuration and psychotomimetic activity. | | 1978 | 101890 |
| cell-mediated and humoral immune responses to cartilage antigenic components. | cell-mediated immunity and antibody production to cartilage antigens were studied in rabbits. from day 3 of immunization an inhibition of leucocyte migration was observed in animals immunized with collagen-free fractions of bovine nasal or human rib cartilage. delayed cutaneous hypersensitivity was elicited between 5 and 6 days and the circulating antibodies were demonstrated by passive haemagglutination on day 9. no significant correlation was observed between the antibody production and the ce ... | 1979 | 105405 |
| cavitary pulmonary lesions complicating use of flow-directed balloon-tipped catheters in two cases. | | 1979 | 106701 |
| carnitine and experimental ketotic and non-ketotic diabetic stupor. | | 1979 | 107105 |
| primary structure of a human iga1 immunoglobulin. iv. streptococcal iga1 protease, digestion, fab and fc fragments, and the complete amino acid sequence of the alpha 1 heavy chain. | in order to establish the complete amino acid sequence of the human iga alpha1 chain bur, iga1 protease from streptococcus sanguis was employed to generate fabalpha and fcalpha fragments in the final stage of this investigation. cyanogen bromide cleavage of the fabalpha fragment followed by reduction and aminoethylation produced the fd' fragment (residues 84 to 227); this contains part of the variable region (vr), the whole first constant domain (calpha1), and part of the hinge region of this he ... | 1979 | 107164 |
| [validity of scintigraphic and endocrinological methods in classifying autonomous adenomas of the thyroid (author's transl)]. | the validity of various scintigraphic and endocrinological methods was assessed in order to classify confirmed autonomous adenomas (aa) and to differentiate aa from euthyroid and hyperthyroid goitres. methods used included: measurement of 131j-uptake 6, 24 and 48 hours after 131j-application, before and after suppression by t3, thyroid scanning, count-rate measurement in the aa and the paranodular tissue, and determination of t4. all patients received 131j-therapy (40,000 rads). in 8 out of 30 s ... | 1979 | 107394 |
| serological diagnosis of systemic candidiasis and invasive aspergillosis. | | 1978 | 107579 |
| [effective methods of obtaining antisera to hemagglutinin h3]. | schedules for production of antisera to pure influenza a virus hemagglutinin (h3) in rats and rabbits are described. the antisera prepared in rats were shown to be better than rabbit sera as they had low levels of inhibitors readily eliminated by heating. the best sera were obtained by immunizations of rats into the tail. both rat and rabbit antisera, in addition to the hi test, can be used satisfactorily in the immunodiffusion test and in radioimmunoassay. | 1978 | 107656 |
| the effects of lipids from listeria monocytogenes on bacterial and fungal infections in mice. | investigations were made on the influence of lipids from listeria monocytogenes upon the course of infections induced by bacteria and fungi. chloroform-methanol extract of the lipids was given to animals in various doses and at various intervals before and after infection. mortality of mice and elimination rate of bacteria from the blood and organs were estimated. it was observed that the lipids markedly increased natural immunity of the animals against bacterial and fungal infections. the morta ... | 1978 | 107921 |
| identification of 15-keto-9, 11-peroxidoprosta-5, 13- dienoic acid as a hematin-catalyzed decomposition product of 15-hydroperoxy-9, 11-peroxidoprosta-5, 13-dienoic acid. | a labile prostaglandin was isolated as one of the products generated from [1-14c] eicosatetraenoic acid incubated with sheep vesicular gland microsomes. the eicosatetraenoic acid metabolite amounted to ca. 16% of the total radiolabeled products. formation of this new prostaglandin was prevented when heat-denatured microsomes were employed or when incubation mixtures were supplemented with indomethacin or phenol. however, incubation of prostaglandin g2 (pgg2) with hematin in the presence or absen ... | 1979 | 108495 |
| the distribution and concentration of thyrotropin-releasing hormone in discrete human hypothalamic nuclei. | | 1979 | 110582 |
| the nature and classification of viruses of man. | | 1979 | 111266 |
| human carotid artery diameter and flow by a noninvasive technique. | this paper presents a technique tested in vitro and in dogs and used in humans to measure carotid artery blood flow continuously by placing a transducer on the skin over the artery. the tranducer consists of a pulse echo crystal used to locate the carotid artery and measure its diameter and wall velocity. it also has a pair of doppler shift crystals used to measure the velocity of the artery wall and blood flow in the vessel. it was found that the pulsatile artery wall distension was between 8 a ... | 1975 | 124007 |
| fenfluramine-induced enhancement of confinement motor activity: an indirect 5-hydroxytryptamine-like action? | | 1975 | 124023 |
| on the specificity of the histochemical technique for sarcoplasmic reticular adenosine triphosphatase: a light and electron microscopic study. | the specificity of the histochemical localization of the calcium activated adenosine triphosphatase (atpase) activity of the sarcoplasmic reticulum (sr) at ph 7.4 was studied using a calcium-citro-phosphate technique. the latter involves the splitting of atp by atpase producing phosphate ions which then react with calcium and citrate to form an insoluble reaction product. this reaction product was detected by both light and electron microscopy. light microscopic examination showed a darkly stain ... | 1975 | 124715 |
| the effect of some bacterial products on temperature and sleep in rat. | the lipopolysaccharides from p. aeruginosa, s. minnesota and mucopeptide from streptococcus group a injected intravenously into rats induce a dose-dependent changes of temperature. simultaneously, a profound disturbance of sleep occurs. the administration of salicylate, which markedly suppressed the fever does not influence the mucopeptide-caused sleep disturbance. the most prominent change in the sleep pattern is a marked decrease of the total time of paradoxical sleep. the measurement of turno ... | 1975 | 126560 |
| proceedings: cardiovascular responses of the anaesthetized cat to lower body negative pressure. | | 1975 | 127635 |
| inhibition of lactic dehydrogenase and of pyruvate kinase by low concentrations of quercetin. | | 1975 | 128007 |
| the effect of increased brain gaba produced by amino-oxyacetic acid on arousal in rats. | previous work has reported behavioral arousal in the rat to be inversely related to cortical gaba production. therefore the effects of an increase in brain gaba levels, induced by amino-oxyacetic acid, on measures of behavioural arousal such as rearing and ambulation were examined. the increase in gaba was immediately associated with decreased rearing, however the behaviour was later indistinguishable from control values while the levels of brain gaba remained raised. it was suggested that the r ... | 1976 | 134388 |
| diminished ouabain-sensitive, sodium-potassium atpase activity in sciatic nerves of rats with streptozotocin-induced diabetes. | | 1976 | 134901 |
| [roentgenmorphologic findings in single homotransplantation of non immunosuppressive treated canine lungs (author's transl)]. | according to plain chest roentgenography correlated to needle biopsies the following grades in the course of rejection after single lungtransplantation could be evaluated. 1) begin of rejection: perihilar alveolar infiltrate. 2) increase of the infiltrates (positive airbronchogram). 3) almost total consolidation of the graft. 4) complete consolidation of the graft, mediastinal hernia caused by the own lung. | 1976 | 134956 |
| [effect of vitamins pp and b1 on pentosephosphate pathway enzymatic activity in the kidneys of adrenalectomized rats]. | the activity of glucose-6-phosphate dehydrogenase (ec 1.1.1.44), transketolase (ec 1.1.1.44), transketolase (ec 1.1.1.49), 6-phosphogluconate dehydrogenase (ec 2.2.1.1.) and also the thiaminediphosphate (tdp) content were investigated on the 8th day after adrenalectomy. with compensated sodium dysbalance the removal of suprarenals is shown not to change the activity of dehydrogenases, but it does lower the transketolase activity and the tdp level. a seven-fold administration to adrenalectomized ... | 1978 | 149433 |
| satellite association in human metaphases. a comparative study of normal individuals, patients with down syndrome and their parents. | | 1979 | 154489 |
| radioligand receptor assay for urinary and serum hcg. | a radioligand receptor assay system for urinary hcg and serum hcg was developed, using 1100-2000 times g fractions of homogenates of pig testis. this method is specific for hcg and lh, and the limit of detection for hcg was 20 miu, which could be significantly discriminated from zero at the 95% confidence level. a nonspecific inhibition reaction which exerts its influence on the assay system has been found in the urine and serum, and in performing assays for urinary and serum hcg, it was necessa ... | 1975 | 165212 |
| rhinosporidium seeberi: spherules and their significance. | this study of the nature and functional significance of the spherules that develop in sporoblasts show them to participate in the germination of rhinosporidium seeberi and are the precursors of the trophocytes. | 1975 | 168546 |
| correlation of estrogen-induced uterine eosinophilia with other parameters of estrogen stimulation, produced with estradiol-17beta and estriol. | estradiol-17beta is a stronger estrogen than estriol for the genomic response of estrogens. estriol is a stronger estrogen than estradiol-17beta for the estrogen-induced uterine eosinophilia and the 6 h increase in the uterine wet weight. | 1975 | 169148 |
| experimental viral infections of the inner ear. i. acute infections of the newborn hamster labyrinth. | acute viral infections of the inner ear were produced in neonatal hamsters. viruses were inoculated percutaneously through the temporal cartilage into the endolymphatic and perilymphatic spaces of the labyrinth or were inoculated intracerebrally to reach the perilymphatic spaces via the cochlear aqueduct. selective vulnerability of inner ear structures was demonstrated using a variety of viruses. influenza virus infected only the mesenchymal cells of the perilymphatic channels of the cochlea; mu ... | 1976 | 177808 |
| occurrence of cross reactions to foot-and-mouth disease virus in normal swine sera. | sera from 101 swine never exposed to foot-and-mouth disease virus were tested by the plaque-reduction neutralization (prn) and radial immunodiffusion techniques for cross-reactions to 5 types of foot-and-mouth disease viruses. depending on the group of sera and the virus used, the percentage of sera cross-reacting at low levels varied from 0 to 50% with the prn technique and 0 to 20% with the radial immunodiffusion technique. 5erum-neutralization tests in mice support the finding of neutralizing ... | 1977 | 201197 |
| effects of 5-(3,3-dimethyl-1-triazeno)imidazole-4-carboxamide, 1-(2-chloroethyl)-3-(4-methylcyclohexyl)-1-nitrosourea, and l-phenylalanine mustard on b16, cloudman s91, and harding-passey mouse melanomas. | the effects of 5-(3,3-dimethyl-1-triazeno)imidazole-4-carboxamide (dtic), 1-(2-chloroethyl)-3-(4-methylcyclohexyl)-1-nitrosourea (meccnu), and l-phenylalanine mustard (l-pam) have been compared by using three i.p. transplanted mouse melanomas: the b16 melanoma in c57bl/6 mice; the harding-passey (hp) malanoma in balb/c x dba/2f1 (hereafter called cd2f1) mice; and the cloudman s91 melanoma in dba/2 mice. hp melanoma responds well to all three drugs. s91 responds only to l-pam and meccnu. dtic may ... | 1979 | 427782 |
| properties of single fibre excitatory post-synaptic potentials in triceps surae motoneurones. | 1. e.p.s.p.s and terminal potentials (t.p.s) produced by the action of single medial gastrocnemius ia afferent fibres were examined with intracellular microelectrodes in cat triceps surae motoneurones. 2. simple terminal potentials (t.p.s) appeared as positive-negative diphasic waves with a single positive peak. shape indices of excitatory post-synaptic potentials (e.p.s.p.s) recorded in conjunction with simple t.p.s indicate that these e.p.s.p.s were generated at an electrotonically confined co ... | 1979 | 529102 |
| ontogeny of the antibody-forming cell line in mice. ii. maturation of b cells during fetal development. | this paper maps the developmental stage reached by b cells in mouse fetuses of different ages. the "maturity" of these cells was assessed in a functional way by measuring the time taken for cells to generate antibody-forming progeny when injected into carrier-primed irradiated adult hosts and challenged daily with antigen. a steady uninterrupted progression in development of b cells occurred between 13 days of gestation and birth. there was no evidence for a stage during which b cells could only ... | 1976 | 1087319 |
| [in vitro sensitivity of trichomonas vaginalis to canestan (clotrimazole)]. | | 1976 | 1087423 |
| strains of pseudomonas putrefaciens from clinical material. | eight strains of pseudomonas putrefaciens have been received from among 466 strains of pseudomonas submitted to the computer trials laboratory for identification over the last eight years. two of the strains of p. putrefaciens from patients with otitis media and otitis externa respectively appear to have played a pathogenic role. the biochemical characteristics of these eight strains were compared with those of seven culture collection strains. | 1975 | 1127110 |
| [modification of the method of determination of fe59 inclusion into the erythrocytes]. | | 1975 | 1196035 |
| effects of external osmolality on polyamine metabolism in hela cells. | the polyamine content of escherichia coli is inversely related to the osmolality of the growth medium. the experiments described here demonstrate that a similar phenomenon occurs in mammalian cells. when grown in media of low nacl concentration, hela cells and human fibroblasts were found to contain high levels of putrescine, spermidine, and spermine. the putrescine content of hela cells was a function of the osmolality of the medium, as shown by growing cells in media containing mannitol or add ... | 1975 | 1201280 |
| [identification of pseudomonas atrofaciens (mc. culloch) stevens by serological method]. | | 1975 | 1214657 |
| basic counterpoint: mechanisms and pathways of gonadal steroid modulation of growth hormone secretion. | in spite of the different patterns of gh secretion observed in male and female rats, it can be argued that there are limited differences between the mechanism of action of androgens and estrogens as reported in the literature. however, we feel that it is possible to organize the available data into a unique physiological model explaining the sex-based differences in gh secretion in the rat. thus, it can be proposed that the greater spontaneous gh peaks observed in the male with respect to the fe ... | 1992 | 1352244 |
| molecular analysis of the gene for the human vitamin-d-binding protein (group-specific component): allelic differences of the common genetic gc types. | dna sequence analysis of the polymerase chain reaction products, including the coding region for amino acids 416 and 420, of the vitamin-d-binding protein (dbp, group-specific component, gc) shows allele-specific differences. the gc2 and gc1f phenotypes have an aspartic acid residue at amino acid position 416, whereas the gc1s phenotype has a glutamic acid at this position. in the gc2 phenotype, amino acid 420 is a lysine residue, and in the both common gc1 phenotypes, it is a threonine residue. ... | 1992 | 1352271 |
| studies on the production of lipase from recombinant staphylococcus carnosus. | production of lipase from recombinant staphylococcus carnosus plipps1 was studied in standard stirred tank bioreactors. only low lipase activity was obtained under conventional operating conditions, i.e., moderate to high stirring speeds and aeration rates for keeping the dissolved oxygen concentration at high levels. additional targetted experiments indicated that the reason for the observed low lipase activity is lipase inactivation due to surface forces and shear stress at the gas/liquid inte ... | 1991 | 1367274 |
| microbiological events during commercial meat fermentations. | microbiological developments during industrial meat fermentations (salami), made with and without commercial starter cultures, were followed at two factories in germany and italy. in the german product microbial growth was evident only for the first 48 h, followed by a gradual decline in numbers of most micro-organisms. the ph fell from 5.8 to 4.8 in the 28 d required for production. in italy a similar situation was seen, except that a second period of bacterial growth began around 15 d, coincid ... | 1992 | 1399914 |
| cloning and molecular characterization of the secy genes from bacillus licheniformis and staphylococcus carnosus: comparative analysis of nine members of the secy family. | secy is a central component of the export machinery that mediates the translocation of secretory proteins across the plasma membrane of escherichia coli. we have cloned and sequenced the secy genes from bacillus licheniformis and staphylococcus carnosus. the deduced amino acid sequences are highly homologous to those of other known secy polypeptides, all having the potential to form 10 transmembrane segments. comparative analysis of 9 secy polypeptides, derived from different bacteria, revealed ... | 1992 | 1435726 |
| escherichia coli heat-labile enterotoxin preferentially interacts with blood group a-active glycolipids from pig intestinal mucosa and a- and b-active glycolipids from human red cells compared to h-active glycolipids. | the capacity of cholera toxin (ct) and of the heat-labile enterotoxin produced by escherichia coli isolated from humans (lth) to interact with glycolipids bearing abo(h) blood group determinants isolated from different sources and separated by thin layer chromatography was studied. toxin binding to the abo(h)-related glycolipids depends on the glycolipid source, the type of the blood group activity, and the toxin. lth and ct were capable of interacting with several blood group-active glycolipids ... | 1992 | 1435767 |
| transforming growth factor beta and basic fibroblast growth factor synergistically promote early bovine embryo development during the fourth cell cycle. | developmentally competent bovine blastocysts were produced by adding transforming growth factor beta (tgf beta) and basic fibroblast growth factor (bfgf) to serum-free cultures of in vitro produced, 2-cell bovine embryos. the effects of tgf beta were evaluated because this growth factor signals synthesis and secretion of the extracellular matrix component fibronectin and its receptor. previous investigations have demonstrated that fibronectin promotes early bovine embryo development in vitro. th ... | 1992 | 1472373 |
| adsorption behaviour of lipase from staphylococcus carnosus on a hydrophobic adsorbent. | the adsorption of proteins on a solid surface with a subsequent desorption is a well known final purification step in downstream processing. here the adsorption behaviour of a microbial lipase on the hydrophobic fractogel tsk butyl 650 in a crude fermentation supernatant is investigated. the measured equilibrium curves differ from fermentation to fermentation by up to +/- 65%. the adsorption capacity increases with decreasing particle diameter of the adsorbent and is influenced by the method of ... | 1992 | 1487509 |
| [effect of environmental temperature on the course of spontaneous postmortem pupillary changes (studies of the rabbit eye)]. | the diameter of the pupils was measured in the bodies of rabbits under different ambient temperatures (2-4 degrees c, 10-12 degrees c, 18-20 degrees c, 28-30 degrees c). it was found that the pupils changed in three phases: initial miosis, which set in within minutes after death, was followed by a mydriatic phase, which lasted several hours and was approximately synchronous with the post-mortem rigidity of the skeletal muscles. in the third phase the pupils slowly contracted in the course of sev ... | 1992 | 1489345 |
| effect of intraduodenal hcl and soybean extract on pancreatic juice secretion during atropinization and cold vagal blockade in calves. | in order to elucidate the mechanisms by which intraduodenal hydrochloric acid (hcl) and soybean extract influence exocrine pancreatic secretion in the young ruminant, we conducted experiments repeatedly on six conscious calves with and without blockade of the extrinsic and intrinsic neural pathways. in the absence of blockade, each of the two stimuli increased the juice volume, the hcl effect being far stronger than that of soybean extract. intrinsic cholinergic blockade by atropinization blocke ... | 1992 | 1489539 |
| expression and regulation of the antimonite, arsenite, and arsenate resistance operon of staphylococcus xylosus plasmid psx267. | the arsenate, arsenite, and antimonite resistance region of the staphylococcus xylosus plasmid psx267 was subcloned in staphylococcus carnosus. the sequenced dna region revealed three consecutive open reading frames, named arsr, arsb, and arsc. expression studies in escherichia coli with the bacteriophage t7 rna polymerase-promoter system yielded three polypeptides with apparent molecular weights of 8,000, 35,000, and 15,000, which very likely correspond to arsr, arsb, and arsc, respectively. ar ... | 1992 | 1534327 |
| mannitol-specific enzyme ii of the phosphoenolpyruvate-dependent phosphotransferase system of staphylococcus carnosus. sequence and expression in escherichia coli and structural comparison with the enzyme iimannitol of escherichia coli. | the enzyme iimannitol (eiimtl) of the phosphoenolpyruvate-dependent phosphotransferase system (pts) catalyses the uptake and concomitant phosphorylation of mannitol by bacteria; it is specified by the gene mtla. mtla is located near the genes mtlf and mtld in the staphylococcal genome, encoding the enzyme iiimtl and the mannitol-1-phosphate dehydrogenase, respectively. we present the cloning of the whole operon by a novel complementation system which is generally suitable for cloning gram-positi ... | 1992 | 1551396 |
| staphylococcal phosphoenolpyruvate-dependent phosphotransferase system: molecular cloning and nucleotide sequence of the staphylococcus carnosus ptsi gene and expression and complementation studies of the gene product. | a digoxigenin-labeled dna probe that was complementary to the gene ptsh and the beginning of the gene ptsi was used to clone a 3.2-kb hincii-bamhi restriction fragment containing the complete ptsi gene of staphylococcus carnosus. the restriction fragment was cloned in the antisense orientation to the lac promoter in the low-copy-number vector psu18. the nucleotide sequences of the ptsi gene, which encodes enzyme i (ec 2.7.3.9), and the corresponding flanking regions were determined. the primary ... | 1992 | 1551842 |
| intrauterine resuscitation in the severely distressed fetal dog. | severe fetal distress was produced in 16 fetal dogs by successive and/or prolonged occlusion of the maternal abdominal aorta in an acute surgical preparation, and was characterized in fetal arterial blood of 7.06 for ph, 10 mm hg for o2, 127 mm hg for co2, and fetal heart rate decelerations. five fetuses recovered spontaneously in utero and delivered normally; 3 needed maternal oxygenation before delivery; 3 suddenly died in utero; 5 fetuses were delivered while still alive in utero when the tph ... | 1992 | 1563650 |
| regulation of staphylococcus xylosus xylose utilization genes at the molecular level. | we have investigated the regulation of the operon encoding xylose utilization in staphylococcus xylosus c2a and staphylococcus carnosus tm300. for in vivo studies, transcriptional fusions of the xylab regulatory region to the lipase gene from staphylococcus hyicus were constructed. repression of lipase activity depended on a functional xylr gene and an xyl operator palindrome downstream of the promoter, while induction was obtained in the presence of xylose. inactivation of either xylr or the xy ... | 1992 | 1569030 |
| rat urate oxidase produced by recombinant baculovirus expression: formation of peroxisome crystalloid core-like structures. | urate oxidase (ec 1.7.3.3), which catalyzes the oxidation of uric acid to allantoin, is present in most mammals but absent in humans and hominoid primates. in rats and most other mammals that catabolize uric acid to allantoin, this enzyme is localized within the crystalloid cores of peroxisomes present in liver parenchymal cells. to determine whether urate oxidase forms these crystalloid cores or whether core-forming protein(s) exist in association with urate oxidase, a baculovirus expression ve ... | 1992 | 1594592 |
| cloning and expression of staphylococcus epidermidis urease gene sequences in staphylococcus carnosus. | the urease gene sequences of staphylococcus epidermidis cns23 were cloned and expressed in staphylococcus carnosus tm300. in vitro translation of the cloned sequences revealed four polypeptides (60, 17, 11 and 7.5 kda) that were associated with enzyme activity. southern hybridisation experiments showed high homologies with the urease genes of staphylococcus saprophyticus. | 1992 | 1612415 |
| an immunocytochemical study of keratin reactivity during rat odontogenesis. | the cytokeratin distribution in the developing rat enamel organ from day 15 of gestation through to 11 days post partum was examined immunohistochemically using a panel of monoclonal antibodies. a temporo-spatial programme of keratin expression was observed during odontogenesis and positive reactivity of the enamel organ was seen with the pan keratin antibodies ck1 (clone lp34 - reacts with a number of keratins including 6 and 18) and ae1-3 (reacts with most acidic and basic keratins). no reacti ... | 1990 | 1698191 |
| analysis of the fine specificities of 11 mouse monoclonal antibodies reactive with type 2 blood group determinants. | the specificity of 11 mouse monoclonal antibodies reacting selectively with type 2 blood group structures was analyzed in detail by studying their reactivities with a panel of standard glycolipids, glycolipids from erythrocytes and blood group glycoproteins. the antibodies reacted with monofucosyl type 2 h, difucosyl type 2 structures (le gamma) or both; none of the antibodies reacted with type 1 (h, lea, or leb) structures. only a small proportion of the antibodies were completely specific for ... | 1990 | 1698257 |
| organization, promoter analysis and transcriptional regulation of the staphylococcus xylosus xylose utilization operon. | the staphylococcus xylosus xyl genes were cloned in staphylococcus carnosus by complementation to xylose utilization. xylose isomerase assays under inducing (xylose present) and non-inducing (xylose absent) conditions indicated the presence of a regulated xyla gene on the recombinant plasmid. the nucleotide sequence (4520 bases) revealed three open reading frames with the same polarity. they were identified by sequence homologies as xylr, encoding the xyl repressor, xyla, encoding xylose isomera ... | 1991 | 1714034 |
| analysis of genes involved in the biosynthesis of lantibiotic epidermin. | the structural gene of the lanthionine-containing peptide antibiotic epidermin is located on a 54-kb plasmid of staphylococcus epidermidis [schnell et al. (1988) nature 333, 276-278]. a 13.5-kb dna region neighbouring the epidermin structural gene (epia) was subcloned and its sequencing revealed five additional open reading frames. three of these reading frames, epib, epic and epid shared no homology with previously described proteins stored in data bases. they were located 3' adjacent to epia. ... | 1992 | 1740156 |
| suppression of an escherichia coli seca(ts) mutant by a gene cloned from staphylococcus carnosus. | escherichia coli mutant mm52 (seca(ts)) was transformed with a cosmid library from staphylococcus carnosus, and a recombinant cosmid (pbo23) allowing growth at the non-permissive temperature (42 degrees c) was isolated. pbo23 also restored the growth defects of e. coli mutants iq85 (secy(ts)) and it41 (lep(ts)). nucleotide sequencing revealed that the dna fragment responsible for the suppression effect codes for a s. carnosus protein highly homologous to the ribosomal protein l13 of e. coli. the ... | 1991 | 1838090 |
| cloning and expression of various staphylococcal genes encoding urease in staphylococcus carnosus. | the urease genes from staphylococcus xylosus c2a, staphylococcus aureus u500, and s. aureus newman were cloned in staphylococcus carnosus using the plasmid vectors pca43 and pca44. the resulting respective recombinant plasmids pura 402, purauh66, and pura17 contained chromosomal dna fragments with sizes of 5.6, 5.8, and 6.8 kb, respectively. investigations on urease expression of the donor and recombinant strains in media with various nitrogen sources revealed that s. xylosus c2a produced urease ... | 1991 | 1884984 |
| staphylococcal phosphoenolpyruvate-dependent phosphotransferase system. purification and protein sequencing of the staphylococcus carnosus histidine-containing protein, and cloning and dna sequencing of the ptsh gene. | the histidine-containing protein (hpr) of the bacterial phosphoenolpyruvate-dependent phosphotransferase system (pts) was isolated from staphylococcus carnosus and purified to homogeneity. the protein sequence was determined by edman degradation of peptides obtained by proteolytic digestion with proteases v8, trypsin and chemical cleavage with brcn. furthermore, immunological screening of a chromosomal s. carnosus dna gene library in puc19 vector enabled us to isolate s. carnosus hpr-expressing ... | 1991 | 1901791 |
| mannitol-specific phosphoenolpyruvate-dependent phosphotransferase system of enterococcus faecalis: molecular cloning and nucleotide sequences of the enzyme iiimtl gene and the mannitol-1-phosphate dehydrogenase gene, expression in escherichia coli, and comparison of the gene products with similar enzymes. | enzyme iiimtl is part of the mannitol phosphotransferase system of enterococcus faecalis. it is phosphorylated in a reaction sequence requiring enzyme i and heat-stable phosphocarrier protein (hpr). the phospho group is transferred from enzyme iiimtl to enzyme iimtl, which then catalyzes the uptake and concomitant phosphorylation of mannitol. the internalized mannitol-1-phosphate is oxidized to fructose-6-phosphate by mannitol-1-phosphate dehydrogenase. in this report we describe the cloning of ... | 1991 | 1904856 |
| diurnal variation in heat production related to some physical activities in laying hens. | 1. heat production, standing and eating activities, and hourly food intake of 4 laying hens were observed simultaneously and the effects of activity and food intake on heat production were studied. 2. average heat production during the dark period (20.00 to 06.00 h) was 18.9 kj/kgw0.75 h which was 33% lower than that during the light period. about 76% of the light-dark difference in the rate of heat production was probably associated with activity and posture. 3. standing time, which included a ... | 1991 | 1933452 |
| ambulatory management of lower respiratory tract infections. | clinical manifestations and radiographic findings are unreliable guides to the selection of antimicrobial therapy for lower respiratory infections. laboratory evaluation is necessary to identify the etiologic agent. multiple oral antibiotics are available for outpatient treatment of bronchitis or pneumonia suspected to be of bacterial origin. | 1991 | 1950984 |
| phosphoenolpyruvate-dependent mannitol phosphotransferase system of escherichia coli: overexpression, purification, and characterization of the enzymatically active c-terminal domain of enzyme iimtl equivalent to enzyme iiimtl. | the extreme c-terminus (ser-490 to lys-637) of the escherichia coli eiimtl was subcloned to test structural and mechanistic proposals about the existence of an eiii-like domain in this enzyme. oligonucleotide-directed mutagenesis was used to produce a unique ncoi restriction site and, at the same time, to change ser-490 into methionine in a flexible region in front of the proposed eiii-like domain. the 16-kda c-terminal domain (ci) was overexpressed in escherichia coli, purified, and analyzed in ... | 1991 | 1993192 |
| exchange of phosphoryl groups between hpr molecules of the phosphoenolpyruvate-dependent phosphotransferase system is an autocatalytic process. | hpr, a central component of the phosphoenolpyruvate-dependent phosphotransferase system, can exist in escherichia coli in a phosphorylated (phpr) and a nonphosphorylated form. we show that, beside the normal transfer of the phosphoryl group from phpr to enzymes ii and iii, phpr can phosphorylate other hpr molecules in an autocatalytic exchange reaction. the reaction is very fast but is inhibited by labeling the protein with bolton-hunter reagent. we demonstrate that the exchange reaction can be ... | 1991 | 2007126 |
| staphylococcus carnosus: a new host organism for gene cloning and protein production. | | 1990 | 2119065 |