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me2+-(13 s) atpase from micrococcus sp. atcc 398e. the effect of trypsin on the purified enzyme.by trypsin treatment of highly purified atpase (ec 3.6.1.3) from micrococcus sp. atcc 398e, two enzyme modifications have been obtained. (i) atpase ta, which has about the same activity as untreated atpase. (ii) a protein complex ti, which lacks atpase activity, but nevertheless binds atp as shown by affinity chromatography. trypsin primarily shortens the alpha-chains of the "native" enzyme to alpha-chains and removes the gamma-subunit, thus yielding atpase ta. the formation of the protein compl ...1976131581
f1-atpase from micrococcus sp. atcc 398. purification by ion-exchange chromatography and further characterization. (auto)proteolysis and dissociative effects.the preparation of highly purified f1-atpase from micrococcus sp. atcc 398 by application of deae-sepharose cl-6b chromatography as final step is described. this enzyme consists of five subunits of different molecular weight: alpha (65000), beta (55000),gamma (35000), delta (20000), and epsilon (17000). disc electrophoresis on 5% polyacrylamide gels removes the epsilon-polypeptide yielding an active atpase complex with four different subunits: alpha, beta, gamma, delta. additionally, by variatio ...1977145365
change in the ratio of cytochrome oxidase activity to nitrite reductase activity of pseudomonas aeruginosa nitrite reductase with the kind of c-type cytochrome used as an electron donor.the ratio between the nitrite reductase and cytochrome oxidase activities of pseudomonas aeruginosa nitrite reductase [ec 1.9.3.2.] varies with kind of c-type cytochrome used as the electron donor. withe cytochrome c-548, 554 (micrococcus sp.), the nitrite reductase activity is greater than the cytochrome oxidase activity, while the former is smaller than the latter with cytochrome c-554 (navicula pelliculosa). the aerobic oxidation catalyzed by this enzyme of denitrifying bacterial ferrocytoch ...1976178646
biological values of the infant, juvenile, and adult agouti (dasyprocta sp) with emphasis on microbial flora.normal values for intestinal flora were determined on four adult, two juvenile, and four infant agoutis (dasyprocta sp) maintained at our institution. serologic, hematologic, biochemical, and histologic observations were also made on these same agoutis, and serologic, microbiologic, and endoparasitic tests were made on serum and fecal samples from agoutis maintained at other institutions. streptococci, lactobacilli and nonenteropathogenic escherichia coli were isolated from cecal contents of all ...1976185456
continuous-culture studies of interactions among human skin-commensal bacteria.chemostat studies were made of an antibiotic-producing staphylococcus epidermidis strain (s6+), a similar, but antibiotic non-producing s. epidermidis strain (s6-), and a sensitive indicator strain of micrococcus sp. (m7). pure and mixed continous cultures were investigated at low population levels (in 0-1% peptone water) and at higher levels (in 0-5% peptone water). strain s6+ antagonised the growth of strain m7 when its colony count was maintained above 10(7-0) per ml, while strain s6- remaine ...1977323493
a special report: four-year study of a boy with combined immune deficiency maintained in strict reverse isolation from birth.a 4-year study of a boy with combined immune deficiency is presented, and the impact of this disease on various aspects of his growth and development is examined. there is no evidence of immune deficiency in either parent or in the genetic background on the maternal side. three children of a brother of the mother's father may have had immune deficiencies but two have grown to be teenagers with no problems. another died. at autopsy, however, lymph nodes appeared normal. the deceased older brother ...1977401538
transformation of mercuric chloride and methylmercury by the rumen microflora.the microflora in strained rumen fluid did not methylate or volatilize 203hg2+ at detectable rates. however, there was an exponential decay in the concentration of added ch3hg+, which was attributed to demethylation. the major product of demethylation was metallic mercury (hg0), and it was released as a volatile product from the reaction mixture. demethylation occurred under both anaerobic and aerobic conditions. the rate of demethylation was proportional to the concentration of added ch3hg+-hg ...1979539820
association between azotobacter and other soil bacteria and its effect on nitrogen fixation.the association between a. vinelandii and either agrobacterium sp. or micrococcus sp., which are usually found as contaminants in azotobacter cultures, was investigated. in comparison with pure cultures, association increased the microbiol counts in addition to increasing nitrogen fixation rates and efficiency. in liquid cultures higher azotobacter densities were observed in the top 5 cm of the column concomitant with lowering the economic coefficient of utilization of carbonaceous compounds, wh ...1979543344
[determination of the molecular weight and radius of gyration of lactobacillus 30a histidine decarboxylase and micrococcus sp. n. histidine decarboxylase]. 1978647048
effect of environmental temperature stress on intramammary infections of dairy cows and monitoring of body and intramammary temperatures by radiotelemetry.four dairy cows were stressed by exposure to hot and cold environments in tests to determine the effect of environment on milk yield, somatic cell counts, and california mastitis test scores of milk from all mammary quarters and on bacterial counts of milk from infected quarters. two cows were held in temperature-controlled rooms for successive 5-day periods at moderate (21 to 28 c), cold (-16 c), moderate, hot (36 to 37 c), and moderate environments. the cold and hot sequences were reversed for ...1977842915
[localization of pyruvic acid in the histidine decarboxylase of micrococcus sp. n].a fragment containing the pyruvic acid residue is isolated from hymotryptic hydrolyzate of alpha-polypeptide chain of carboxymethylated histidine decarboxylase (hd) 14c-phenylhydrasone. the pyruvate residue is estimated to be bind with alpha-amino group of n-terminal phenylalanine in alpha-polypeptide chain. n-terminal alanine is found in hd alpha-polypeptide chain after its reductive amination. it makes possible to determine the estimation of n-terminal amino acid sequence in hd alpha-chain.1976974173
[amino acid sequence in tryptic peptides of maleylated micrococcus sp. n. histidine decarboxylase beta-polypeptide chain].maleilated histidine decarboxylase beta-polypeptide chain, containing 3 arginine residue, was hydrolysed by trypsin. 4 non-overlapping homogenous peptides were isolated, 3 of them containing one arginine residue and the 4th peptide being c-terminal fragment of beta-chain. beta-polypeptide chain is found to consist of 78 amino acid residues and to have molecular weight of 8456. primary structure of each peptide and their possible sequence in beta-chain are determined.19761024578
influence of temperature and salt concentration on the growth of a facultatively halophilic "micrococcus" sp.growth characteristics of a facultatively halophilic strain of micrococcus, capable of growth in medium containing no added nacl and in medium containing 5.5 m nacl, were studied over a range of salt concentrations and temperatures. optimal growth occurred at 35c in a medium containing 1.0 m nacl. in a medium containing 0.1 m nacl, growth only occurred if the incubation temperature was less than 30c. no growth occurred if kcl, licl, nh4cl, mgcl2, or sucrose was substituted for nacl at 35c. a ree ...19751116036
antibacterial activity of essential oil components.antibacterial activity of fifteen essential oil components towards food borne staphylococcus sp., micrococcus sp., bacillus sp. and enterobacter sp. was studied by an agar plate technique. cinnamic aldehyde was the most active compound followed by citral, geraniol, eugenol and menthol. at 500 micrograms/ml, cinnamic aldehyde completely inhibited the bacterial growth for more than 30 days at 30 degrees c that was comparable to 200 micrograms/ml of butylated hydroxy anisole (bha). at lower tempera ...19921457292
cloacal flora isolated from wild black-bellied whistling ducks (dendrocygna autumnalis) in laguna la nacha, mexico.cloacal swabs from 110 adult black-bellied whistling ducks trapped at laguna la nacha, tamaulipas, mexico, were cultured to determine the prevalence of normal and potentially pathogenic bacteria. twenty-five gram-negative enterobacteria and four gram-positive cocci were isolated. the most common isolates included escherichia coli (54%), staphylococcus spp. (29%), streptococcus spp. (22%), aeromonas hydrophila (15%) enterobacter cloacae (14%), and micrococcus sp. (14%). the implications of whistl ...19921627117
the metabolism of anthracene and 9,10-dimethylanthracene by bacteria isolated from waters.the metabolism of two polycyclic aromatic hydrocarbons i.e. anthracene and 9,10-dimethylanthracene by micrococcus sp., pseudomonas sp. and bacillus macerans was examined. the above compounds were used as a sole carbon source for their growth. using the reversed-phase thin layer chromatography techniques a number of anthracene and 9,10-dimethylanthracene metabolites were isolated and their structures identified spectroscopically. these included anthracene and 9,10-dimethylanthracene cis-dihydrodi ...19911726622
palladium, platinum, cadmium, and mercury complexes with neutral isoorotic and 2-thioisoorotic acids: ir and nmr spectroscopies, thermal behavior and biological properties.seven complexes containing neutral isoorotic and 2-thioisoorotic acids, as well as thiocyanate and chloride anions as lignands, have been synthesized and characterized by means of both spectral (ir, 1h, and 13c nmr) and thermal (tg and dsc) methods, as well as conductivity measurements. spectral data suggest that any binding metal-ligand mode for uracil derivatives is not easy to propose. therefore, isoorotic ligands must link through some oxygen atom. likewise, 2-thioisoorotic acid seems to be ...19911940899
[milk chloride level as an indicator of bovine mastitis].the aim of this study was to determine an influence of udder infection on milk chloride level and on milk productivity of cows of black and white race. bacteriological analysis was performed by bacterial isolation from milk collected in sterile conditions from single lobes of mammary gland. the study was aimed to detect the following bacteria: streptococcus agalactiae, streptococcus uberis, streptococcus agalactiae, staphylococcus aureus, staphylococcus epidermidis, actinomyces pyogenes, enterob ...19902084451
bacterial production of histamine in some tropical fish.quantitative and qualitative distribution of histamine-forming bacteria associated with the fish rastrelliger kanagurta, sardinella longiceps, sillago sihama and liza subviridis, were investigated. these bacteria constituted a significant portion of the total bacterial population of fish and the values obtained in the present study were higher than those previously reported. the order of quantitative abundance of histamine-forming bacteria in the fish examined was: s. longiceps greater than r. k ...19902259288
the nucleotide sequence of an alpha-amylase gene from an alkalopsychrotrophic micrococcus sp.an alpha-amylase gene from micrococcus sp. 207 was cloned into escherichia coli jm101 using the vector phsg399. the constructed recombinant plasmid pyk63 contained a 4.8 kb chromosomal dna fragment derived from strain 207 dna. the cloned amylase isolated from e. coli jm101 (pyk63) produced mainly maltotetraose from starch, and exhibited temperature and ph activity profiles closely similar to those of the enzyme from the original strain. nucleotide sequence analysis of the cloned dna fragment rev ...19902276614
stomatococcus mucilaginosus bacteremias. typical case presentations, simplified diagnostic criteria, and a literature review.even though stomatococcus mucilaginosus is considered indigenous oral-pharyngeal flora, cited literature and case reports indicate that it can be the cause of infectious conditions. tested strains were isolated from blood, the oral region, and wound sources. the organism was routinely misidentified or not identified by conventional or commercial systems (vitek, staph-trac). four antimicrobial diagnostic disks for example, bacitracin (0.04 units; taxo a), furazolidone (100 micrograms), novobiocin ...19902279383
antistaphylococcal activity of pentamidine.pentamidine isethionate was bacteriostatic against staphylococcus aureus, staphylococcus epidermidis, streptococcus pyogenes, streptococcus sanguis, micrococcus sp., and candida albicans. s. aureus was inhibited by concentrations of 16 to 64 micrograms/ml and killed by 64 to greater than or equal to 128 micrograms/ml. staphylococcal killing was consistently greater in the presence of cations and was unaffected by methicillin resistance.19902285292
antimicrobial activity of stratum corneum lipids from normal and essential fatty acid-deficient mice.among the cutaneous effects of an essential fatty acid deficient (efad) diet are hyperdesquamation, increased transepidermal water loss (tewl), and altered lipid profiles, characteristics also common to inflammatory dermatoses. because fatty acids are antimicrobial, we examined the indigenous skin flora of normal and efad hairless mice, and compared the antimicrobial efficacy of lipids extracted from their stratum corneum. efad mice supported 100-fold more bacteria than normal mice, and were the ...19892649598
[the asepsis problem at the office of the ent physician].microorganisms were determined in a microbiological study in the near environment of ent working places. we found especially saprophytic flora as micrococcus sp., staphylococcus epidermidis and aerobic spore-forming microorganisms on 54 examination instruments of 2 outpatient and 2 inpatient treatment areas. in 22% of the instrument examinations, microbiological controls were negative. there were no pathogenic microorganisms.19892653348
[typing of skin staphylococci using the commercial identification system staphy-test].the authors examined 150 strains of s. aureus and 180 strains of s. epidermidis isolated from different dermatoses. the determination of s. aureus and s. epidermidis was based on common routine tests as plasmacoagulase, production of hemolysin, acid production from mannitol and production of pigment. this determination was verified with the aid of a new identification system staphytest. the staphytest confirmed the previous diagnosis of s. aureus in 60.66% and that of s. epidermidis in 31.66% of ...19892676626
screening of isoquinoline alkaloids and their derivatives for antibacterial and antifungal activities.a screening test of antimicrobial activities for some of the isoquinoline alkaloids and their first and second hofmann elimination products was conducted in this study. results showed that (+)-actinodaphnine (1), (+)-n-me-actinodaphnine (2), (+)-anonaine (17), (-)-xylopine (19) and (-)-n-me-xylopine mei (20), had the strongest inhibitory activities against three g(+) bacteria (bacillus cereus, micrococcus sp. and staphylococcus aureus) mic greater than or equal to 50 micrograms/ml). whereas anhy ...19892733073
[aerobic bacterial flora of the nasal cavity of rabbits].on the basis of bacteriological examinations carried out in april 1984 on 60 intranasal swabs, aerobic respiratory microbes were studied in rabbits. differences in flora between animals with and without respiratory diseases were studied. fourteen bacterial species were identified with no difference due to the pathological status. they were: bordetella bronchiseptica, staphylococcus epidermidis, streptococcus faecalis, pasteurella multocida, staphylococcus aureus, bacillus sp., branhamella catarr ...19863096189
elimination of mercury, cadmium and antibiotic resistance from acinetobacter lwoffi and micrococcus sp. at high temperature.resistance determinants for hgcl2 and cdcl2 were eliminated along with a number of antibiotic resistance factors from acinetobacter lwoffi and micrococcus sp. at 44 degrees c. these organisms were orginally resistant to hgcl2, merbromin, cdcl2, pb(no3)2, benzylpenicillin, erythromycin, carbenicillin, tetracycline and sulfadiazine. four different types of mutants from a. lwoffi (type i to iv) and one type of mutant from micrococcus sp. (type v) were obtained, depending on the loss of particular r ...19883397010
biotransformation of 3-methylphthalate by micrococcus sp. strain 12b.when micrococcus strain 12b grown on o-phthalate was incubated with 3-methylphthalate, three compounds accumulated. these were shown to be 2-pyrone-3-methyl-4,6-dicarboxylic acid, 3,4-dihydroxy-6-methylphthalic acid, and 5-hydroxy-3-methyphthalic acid, all previously undescribed. a pathway for the formation of these compounds is proposed.19873448154
ofloxacin for prevention of bacterial infections in granulocytopenic patients.we studied the potential value of oral ofloxacin (200 mg twice daily) for selective decontamination and infection prevention in 40 granulocytopenic patients with acute leukemia, blast crisis of chronic myelogenous leukemia, hairy cell leukemia or severe aplastic anemia. the quality of selective decontamination was acceptable with rapid elimination of enterobacteriaceae from the alimentary tract, only a slight decrease in concentrations of anaerobes in faeces, and a small number of newly acquired ...19873481358
evaluation of selective media for primary isolation of treponema hyodysenteriae and treponema innocens.a total of 2450 samples of feces, intestinal contents and colon mucosal scrapings were bacteriologically examined. a total of 53 strains of treponema sp. were isolated, and 45 strains of bacteroides sp., 30 strains of e. coli, 30 strains of micrococcus sp. and 10 strains of streptococcus d isolates were randomly selected. growth promoting studies showed statistically significant stimulation of treponema sp. growth by yeast extract, chicken egg yolk and rumen fluid. different growth inhibitors we ...19863533408
microbiology of the genitalia of nulliparous and postpartum savanna brown goats.a study of the bacterial flora of the genitalia of nulliparous savanna brown does was carried out both before breeding and at different intervals postpartum to investigate the type of microbial organisms that could be present in the uterus, cervix and the vagina respectively. of 29 pre-breeding vaginal swabs, staphylococcus sp. was isolated from 20 goats, streptococcus sp. from 15 goats and micrococcus sp. from four goats. mycoplasma agalactiae was isolated from five goats. the postpartum vagina ...19873590621
the nosocomial colonization of t. bear.a national effort to reduce nosocomial infections includes a program developed at the national institutes of health to encourage handwashing in hospitals and day care centers. the program promotes a symbolic teddy bear (t. bear) with slogans to remind hospital personnel and patients to practice handwashing. one of the items used is a stuffed toy t. bear to be dispensed to the hospitalized child. considering the manner in which children handle stuffed toys, we suspected the t. bear might serve as ...19863640737
[effect of norfloxacin on bacterial flora in human feces].norfloxacin (nflx), a synthetic oral antibacterial agent of quinolone carboxylic acid, was given orally for 5 full days at a dose of 200 mg, three times daily after each meal to healthy normal men with ages between 22 and 25 years weighing 53.0 to 84.0 kg (average 67.0 kg). the actual regimen followed was that the drug was administered twice after lunch and supper on the first day of dosing, and once after breakfast on the last day of dosing. on the 5th day before the start of dosing, on the fir ...19873694785
[effect of s6472 and cefaclor on bacterial flora in adult human feces].s6472 is a mixture of cefaclor (ccl) granules with gastric-soluble coating and those with enteric-soluble coating at the ratio (in potency) of 4 to 6. with administration of this formulation, a prolonged blood level of ccl is obtained. s6472 and ccl were administered orally to 19 healthy male volunteers between 20 and 27 years of age (mean: 23 years) weighing between 51 and 80 kg (mean: 64.7 kg). four subjects were given 2 capsules and 5 subjects were given 4 capsules each containing 187.5 mg of ...19863702058
[effect of cefminox on bacterial flora in human adult feces].cefminox (cmnx), a new cephamycin, was administered by one shot intravenous injection twice daily with a dose of 1,000 mg each time for 5 days to seven healthy male volunteers whose ages ranged from 21 to 28 years (mean: 25 years) and body weights were from 60 to 92 kg mean: 72 kg). the effect of the drug on fecal bacterial flora was investigated and the concentrations of the drug in feces were measured on 5th day before the treatment, on 0, 3rd, and 5th day (the final day of the treatment) duri ...19863761549
quantitative determination of catalase activity produced by neisseria gonorrhoeae, staphylococcus epidermidis, neisseria meningitidis and other bacterial strains using the catalasemeter.the conventional catalase test only gives qualitative or semi-quantitative information of the amount of catalase activity produced. using such a method, we have selected two strong catalase activity producers (neisseria gonorrhoeae g-10, staphylococcus epidermidis 66) and a weak producer (neisseria meningitidis 34702). quantitative determination of the catalase activity produced by these strains was done by the disk-flotation method using the catalasemeter. the production of catalase activity in ...19853924653
a note on antibiotic resistance in the bacterial flora of raw sewage and sewage-polluted river tigris in mosul, iraq.polluted water samples collected from the river tigris in the vicinity of a raw sewage outfall were examined for the incidence of antibiotic resistance among coliform bacteria on three occasions during 1983. eighty percent or more of the coliform bacteria were resistant to one or more antibiotics. at the same time, raw sewage samples were examined for the incidence of antibiotic-resistant bacteria, and escherichia coli, pseudomonas spp. and staphylococcus spp. were selected for sensitivity testi ...19853997692
aerobic bacterial flora from dead-in-shell chicken embryos from nigeria.dead-in-shell chicken embryos from two commercial hatcheries in anambra state of nigeria were investigated for isolation of aerobic bacteria. for this purpose, 79 pooled samples containing 632 dead-in-shell chicken embryos were cultured. from these samples, 23 isolates of escherichia coli and 25 isolates of staphylococcus aureus were recorded. other bacterial species isolated included micrococcus sp. (fifteen isolates), klebsiella sp. (thirteen isolates), pseudomonas sp. (nine isolates), and pro ...19854074232
[partition of histidine decarboxylase subunits from micrococcus sp. n. and their physiocochemical characteristics]. 19744461043
oropharyngeal cultures of patients in protected environment units: evaluation of semiquantitative technique during antibiotic prophylaxis.a semiquantitative culture technique was used to monitor the microbial flora of the oropharynx of 30 patients receiving antibiotic prophylaxis in protected environment units. after institution of antibiotic prophylaxis, the median concentration of organisms in the oropharynx fell by 2 logs but gradually increased by 1 log and then remained stable. neisseria spp., micrococcus sp., and streptococci were generally eradicated by the antibiotics but were replaced by lactobacilli and yeast. four of ni ...19724555635
[study of the prosthetic group of histidine decarboxylase of micrococcus sp. n]. 19734780974
separation and characterisation of subunits of histidine decarboxylase from micrococcus sp.n. 19744829439
distribution of the isopropylmalate pathway to leucine among diverse bacteria.alpha-isopropylmalate synthase and beta-isopropylmalate dehydrogenase activities were detected in extracts of the following organisms: chromatium d, rhodopseudomonas spheroides, hydrogenomonas h16, pseudomonas aeruginosa, pseudomonas fluorescens, vibrio extorquens, rhizobium japonicum, alcaligenes viscolactis, escherichia coli b, proteus vulgaris, aerobacter aerogenes, salmonella typhimurium, micrococcus sp., micrococcus lysodeikticus, bacillus polymyxa, bacillus subtilis, and nocardia opaca. th ...19744829932
[antibiotic activity in vivo of a staphylococcin produced by a strain of micrococcus sp]. 19684899171
inhibition of the plaquing efficiency of t4r+ bacteriophage by subtilisin.the mechanism by which the replicative cycle of t4r(+) phage is inhibited by certain nonhost bacterial systems was investigated. some bacillaceae, especially bacillus subtilis, decreased the plaquing efficiency of this virus more than 95% within 24 hr of exposure. sarcina lutea and micrococcus sp. both failed to cause any significant change in the infectivity of t4r(+) phage. preliminary investigations into the nature of the inhibitory substance(s) suggested that an extracellularly elicited prot ...19714995848
evidence for extreme uv resistance of micrococcus sp. nctc 10785. 19725029860
[study of the mechanism of in vitro inhibition of a micrococcus sp strain by a staphylococcus pyogenes strain]. 19695373662
[in vitro study of the mechanism of inhibition of a staphylococcus pyogenes strain by a micrococcus sp strain]. 19695373663
[4-imidazolyl-3-aminobutanone-2--new inhibitor of histidine decarboxylase (gdc) from micrococcus sp. n]. 19695398202
[preparative isolation of crystalline histidine decarboxylase from micrococcus sp. n]. 19705498657
[photosensitized oxidation of histidine decarboxylase from micrococcus sp. n]. 19705501670
[some da on the structure of histidine decarboxylase from micrococcus sp. n]. 19705501671
nuclease production and lysostaphin susceptibility of staphylococcus aureus and other catalase-positive cocci.some strains of staphylococcus epidermidis and micrococcus sp. produce nucleases. however, thermal stability was shown to be unique to the nucleases of s. aureus. in addition, two micromethods for susceptibility testing to lysostaphin were more precise and convenient than anaerobic glucose fermentation in distinguishing between the genera staphylococcus and micrococcus.19715574317
[c-terminal sequence of amino acids in histidine decarboxylase from lactobacillus 30a and micrococcus sp. n]. 19675599815
[the amino acid pattern of crystalline histidine decarboxylase of micrococcus sp. n. and lactobacillus 30a]. 19685678148
[on the antibacterial activity of a strain of micrococcus sp. in regard to staphylococcus aureus; compared with antibacterial activity of 24 antibiotics of current use]. 19655861162
mode of attack on phenol by a micrococcus sp. isolated from coir rets. 19665972637
characterization of a monoclonal antibody specific for lipoteichoic acid from various gram-positive bacteria.a hybrid cell line, 3g6, producing monoclonal antibody (mab) against the polyglycerophosphate (pgp) backbone of lipoteichoic acids has been derived by the polyethylene glycol-induced fusion of mouse myeloma cells and spleen cells from mice immunized with partially purified glucosyltransferase from culture supernatant of streptococcus mutans strain 6715. immunodiffusion tests and elisa revealed that the antibody reacted with purified pgp from group a streptococcus pyogenes strain sv as well as cr ...19846083437
[spoilage microorganisms encountered in ultra-high temperature processed milk].40 strains of aerobic or facultative anaerobic microorganisms were isolated from a total of 37 spoiled ultra high temperature processed milk. 13 of them were identified as the genus bacillus. they were 6 b. cereus, 5 b. licheniformis, 1 b. brevis and 1 b. pumilus. the other 27 strains were nonsporeforming microorganisms, which included 5 yeasts, 2 pseudomonas sp., 3 streptococcus sp., 12 lactobacillus sp., 1 shigella sp., 1 aeromonas sp. and 3 micrococcus sp. results indicate that the spoilage o ...19846468029
scanning electron microscopy of dairy equipment surfaces contaminated by two milk-borne micro-organisms.ethanol dehydration followed by argon replacement induced drying (arid) was found to be a suitable method for the preparation of glass, stainless steel and rubber surfaces which had been in contact with inoculated milk and which were to be examined using scanning electron microscopy (sem). this technique was used to examine samples of all three materials which had been subjected to both single and repeated inoculation with whole milk containing a pseudomonas sp. or a micrococcus sp. and incubate ...19846490557
reversible inhibition of bacterial growth after specific inhibition of spermidine synthase by dicyclohexylamine.the effect of dicyclohexylamine on seven freshly isolated bacterial strains of mastitis pathogens was studied. streptococcus uberis was the most sensitive strain investigated, since 5 mm-dicyclohexylamine totally arrested its growth and 1.25 mm of the drug caused 60% growth inhibition. the staphylococcus aureus, escherichia coli and pseudomonas aeruginosa strains were also sensitive to the drug, but less so than strep. uberis, since 5 mm drug caused only partial inhibition of growth. micrococcus ...19846508744
neutral lipids in the study of relationships of members of the family micrococcaceae.the organisms studied were those of the family micrococcaceae which cannot participate in genetic exchange with micrococcus luteus and those whose biochemical and physiological characteristics appear to bridge the genera staphylococcus and micrococcus. the hydrocarbon compositions of m. luteus atcc 4698 and micrococcus sp. atcc 398 were shown to be similar to those previously reported for many m. luteus strains, consisting of isomers of branched monoolefins in the range c25 to c31. however, micr ...19715122809
halophilic amylase from a moderately halophilic micrococcus.a moderately halophilic micrococcus sp., isolated from unrefined solar salt, produced a considerable amount of extracellular dextrinogenic amylase when cultivated aerobically in media containing 1 to 3 m nacl. the micrococcus amylase had maximal activity at ph 6 to 7 in 1.4 to 2 m nacl or kcl at 50 c. calcium ion and a high concentration of nacl or kcl were essential for activity and stability of the amylase. the salt response of the amylase depended greatly on the ph and temperature of the enzy ...19725058445
[proteolytic resistance and thermostability of catalase and histidine decarboxylase from micrococcus sp. n].catalase from micrococcus sp. n. is not hydrolyzed by trypsin at the protein/protease (pn/pe) exceeding 10/1. histidine decarboxylase (hdc) loses 50% of activity after the first nine minutes of hydrolysis at the pn/pe ratio equal to 6/1, followed by a slow linear inactivation. investigation of thermal stability at varying ph and temperatures demonstrated that catalase and hdc preserve 70-80% of activity after 5 minutes of incubation at 72 degrees c only at ph approaching the optimal ph of enzyma ...19846722300
a note on the temperature tolerance of legionella.a strain of legionella pneumophila serogroup 1 isolated from the environment had a decimal reduction time in water at 50 degrees c (d50) of 111 min, a d54 of 27 min and a d58 of 6 min. there was little loss of viability at 46 degrees c. other environmental organisms, a pseudomonas sp., a micrococcus sp. and a coliform survived less well at these temperatures. a species of sarcina had a survival time greater than the l. pneumophila at all the temperatures tested. other strains of legionellas were ...19846725165
microbiological sampling of towels: comparison of 7 methods.pull-out roller towels were infected with bacteria and examined with 5 non-destructive methods. in addition to three traditional methods, the agar contact method (a), the swab method (s) and the tape method (t), two lesser known methods have been applied: the rinsing box method (r), new developed in the laboratory, and the filter method (f). in the first (r), the towel is fixed between the lid and the cup of a metal box containing a rinsing liquid; shaking the box completes the test. in the seco ...19826750989
a polymer of glucose and n-acetylgalactosamine 1-phosphate in the wall of micrococcus sp. a1.1. the walls of micrococcus sp. a1contain about 43% of a phosphorylated polymer. it was extracted with cold trichloroacetic acid and purified by chromatography on deae-cellulose. 2. the polymer contained equimolar amounts of d-glucose, n-acetylgalactosamine and phosphate, and was readily hydrolysed under gentle acidic conditions to a phosphorylated disaccharide. 3. chemical and enzymic degradation indicated that this was 3-o-alpha-d-glucopyranosyl-n-acetylgalactosamine with a phosphomonoester gr ...19714329871
[localization of cysteine residues in the alpha-chain of histidine decarboxylase from micrococcus sp. n].it was shown that the alpha-chain of histidine decarboxylase of micrococcus sp. n. is split off by 2-nitro-5-thiocyanobenzoic acid at only one of the two cysteine residues. determination of the c-terminal sequences, amino acid composition, molecular weight of the fragments obtained demonstrated that these fragments constitute a complete alpha-chain whose cleavage occurs at the cysteine residue which is readily modified by sh-reagents. the ile-cys peptide bond appeared to be resistant to cleavage ...19863730446
rapid identification of staphylococcus aureus and streptococcus pneumoniae from blood cultures.simultaneous application of the lysostaphin sensitivity test for identification of staphylococcus aureus and the deoxycholate test for the identification of streptococcus pneumoniae was evaluated for reliability in rapid identification (1 h) of these organisms from blood cultures by using bactec 6b and 7c bottles. the procedure was applied to 127 cultures, 74 lysostaphin tests and 53 deoxycholate tests. lysostaphin-tested organisms included 23 s. aureus, 40 staphylococcus epidermidis, 1 listeria ...19836826713
[kinetic properties and functional role of domains of micrococcus sp n. histidine decarboxylase]. 19836838927
[evaluation of bactericidal activity of antiseptics on the cutaneous flora of holoxenic hairless mouse].holoxenic hairless mice were used in an experimental model to study the in vivo bactericidal activity of several antiseptics. in these experiments skin was sampled by biopsy; after contact between antiseptic and skin, bactericidal activity was neutralized. the results obtained with several products (polyvinylpyrrolidone iodine, alcoholic iodine, ethanol, chlorhexidin, benzalkonium chloride, mercurothiolic acid, trichlorocarbanilid and hexachlorophen) were quite similar to those obtained on human ...19836847033
purification and characterization of extracellular caseinolytic enzyme of micrococcus sp. mcc-315 isolated from cheddar cheese.micrococcus sp. mcc-315, an organism isolated from cheddar cheese, produced an extracellular calcium metalloenzyme. this protease was purified to homogeneity from culture supernatant by precipitation with ammonium sulfate (50 to 70% saturation) and gel filtration through sephadex g-100, resulting in about 82 times increase of specific activity and 53% recovery of the enzyme. the protease exhibited a ph optimum at 10.6 for both whole casein and beta-casein. it had optimum activity for whole casei ...19863519702
metabolism of dimethylphthalate by micrococcus sp. strain 12b.during growth of micrococcus sp. strain 12b with dimethylphthalate, 4-carboxy-2-hydroxymuconate lactone (chml, x) and 3,4-dihydroxyphthalate-2-methyl ester (xi) were isolated from culture filtrates. chml is the lactone of intermediate 4-carboxy-2-hydroxymuconate (ix). accumulation of xi which is not a substrate for 3,4-dihydroxyphthalate-2-decarboxylase in strain 12b afforded an easy access to the preparation of 3,4-dihydroxyphthalate.19827085569
metabolism of dibutylphthalate and phthalate by micrococcus sp. strain 12b.micrococcus sp. strain 12b was isolated by enriching for growth with dibutylphthalate as the sole carbon and energy source. a pathway for the metabolism of dibutylphthalate and phthalate by micrococcus sp. strain 12b is proposed: dibutylphthalate leads to monobutylphthalate leads to phthalate leads to 3,4-dihydro-3,4-dihydroxyphthalate leads to 3,4-dihydroxyphthalate leads to protocatechuate (3,4-dihdroxybenzoate). protocatechuate is metabolized both by the meta-cleavage pathway through 4-carbox ...19827085570
[tryptic peptides of maleylated alpha-chain of histidine decarboxylase from micrococcus sp. n].the maleylated alpha-chain of histidine decarboxylase from micrococcus sp. n., containing 10 arginine residues was hydrolyzed by trypsin, and 9 peptides were isolated from the tryptic hydrolysate. a comparative study of the amino acid sequence in the tryptic peptides of alpha- and beta-chains of histidine decarboxylase allowed to establish the intramolecular homology in the alpha-chain primary structure and homology between the alpha- and beta-chains.19817248384
simple method to demonstrate radiation-inducible radiation resistance in microbial cells.a simple method for detection of radiation-inducible radiation resistance was developed by irradiating aliquots (0.01 ml) of cell suspension on agar plates. part of each experimental plate was subjected to an induction treatment, and subsequent radiation resistance was compared with that of untreated cells on the same plate. the uv radiation resistance of a micrococcus sp. was increased approximately 1.6 times by an induction treatment. this simple procedure of irradiating cells in a "fixed" pos ...19863513701
a marine micrococcus produces metabolites ascribed to the sponge tedania ignis.extracts of the sponge tedania ignis have been reported to contain several diketopiperazines. as part of an investigation of the commensal and symbiotic microflora of sponges, we have consistently isolated, from specimens of t. ignis, a micrococcus sp. which produces diketopiperazines in laboratory cultural media. this is the first demonstration that a bacterium associated with a sponge produces secondary metabolites ascribed to the sponge host.19883197807
[primary structure of histidine decarboxylase].the results of investigation of the primary structure of the histidine decarboxylase micrococcus sp. n. are reported. a comparison of the primary structure of the histidine decarboxylase micrococcus sp. n. with that of the lactobacillus 30a enzyme suggests the alignment with a 52% identity. it is therefore highly probable that two proteins have evolved from common ancestry. the conservative amino acid sequences with residues (pyruvate, cysteine) of the active center have been found.19892914171
[etiology of bacterial endocarditis in materials from cardiology and cardiac surgery clinics of the lodz academy].in the years 1986-1991, 72 cases of bacterial endocarditis (be) were investigated. the bacteria were isolated from blood in 24 cases, from native and artificial valves obtained during surgery in 24 cases, and from both blood and valves in 6 cases. following microorganisms were isolated: s. epidermidis, s. xylosus, s. hominis, s. aureus, micrococcus sp., s. mitis, s. sanguis, s. faecalis, m. lacunata, e. adecarboxylata, p. maltophila, corynebacterium sp., a. calcoaceticus var. lwoffii, and asperg ...19938189810
substrate-mediated purification and characterization of a 3-hydroxybenzoic acid-6-hydroxylase from micrococcus.3-hydroxybenzoic acid-6-hydroxylase from micrococcus sp. was purified to homogeneity in a single step using the substrate-mediated interaction of the enzyme with blue-sepharose. the enzyme was bound to the affinity matrix in the presence of 3-hydroxybenzoic acid and was eluted in its absence. the molecular weight of the purified enzyme is 70,000 with no subunit structure. the flavoenzyme required the exogenous addition of fad for its complete activity and had a strict preference for nadh over na ...19902321959
effect of the fungicides tridemorph and vinclozolin on soil microorganisms and nitrogen metabolism.the effects of tridemorph and vinclozolin were studied on different types of microorganisms, urea hydrolysis and nitrification in soil and in culture. the fungicides adversely affected the population of bacteria, fungi and actinomycetes as a function of time of incubation. urea hydrolysis both in culture and soil were also inhibited by the fungicides, and tridemorph was more detrimental. in soil, 45 micrograms/g of tridemorph inhibited 50% of ammonification of urea, id50 for nitrite production w ...19911841872
evaluation of skin bacterial flora before and after aseptic preparation of clipped and nonclipped arthrocentesis sites in horses.this study evaluates skin bacterial flora before and after aseptic preparation of clipped and nonclipped arthrocentesis sites in horses.19979068162
comparison of three commercial rapid agglutination test kits for identification of coagulase positive staphylococci from foods and animals.three rapid agglutination assays for the identification of staphylococcus aureus monostaph (bionor a/s, skien, norway), staphyslide-test (biomerieux, lyon, france) and staph-rapid-test (roche, basel, switzerland), were compared. a total of 104 gram-positive, catalase positive cocci were tested: nineteen staphylococcus reference strains comprising 15 spp. (4 strains were coagulase positive), and 7 micrococcus reference strains comprising 4 spp.; 22 food isolates comprising 13 s. aureus, 8 coagula ...19911803928
microbial keratitis in patients infected with the human immunodeficiency virus.posterior segment complications of systemic infection with the human immunodeficiency virus (hiv) are well recognized. the anterior segment complications often are, however, overlooked. the author treated 20 episodes of nonherpetic infectious keratitis in 17 eyes of 13 patients infected with hiv who presented between august 1990 and may 1994.19959121747
[specific modification of free lysine amino groups of histidine decarboxylase from micrococcus sp. n. by trinitrobenzene sulfonic acid].it has been found that 14 lysine residues are accessible for trinitrobenzene sulfonic acid (tnbs) in the molecule of histidine decarboxylase (hdc). the other 62 lysine residues in the molecule of native hdc are masked and inaccessible for tnbs. it is demonstrated that the sh- and alpha-amino groups of methionine are not modified by tnbs. a correlation between the decarboxylase activity of the enzyme and the degree of its trinitrophenylation has been studied. hdc, whose molecule contains 3--9 tnp ...19761022283
antimicrobial activity of food-related penicillium sp. against pathogenic bacteria in laboratory media and a cheese model system.food-related penicillium species (n = 34) and geotrichum candidum (n = 11) grown on czapek dox and brie agar were tested for their ability to suppress growth of pathogenic bacteria. ten out of 13 p. camemberti showed antagonistic activity while the other species did not interact significantly with the bacterial growth. the order of inhibition was: gram-negative bacteria and bacillus cereus > listeria monocytogenes, lactococcus sp. > micrococcus sp. whereas lactobacillus sp., staphylococcus aureu ...19979246777
detection of erythromycin resistant methylase gene by the polymerase chain reaction.a polymerase chain reaction (pcr) protocol was developed that could specifically amplify a 520-bp region of the erythromycin resistant methylase (ermc) gene sequence. the identity of the pcr-amplified 520-bp dna was confirmed by hincii endonuclease restriction digestion, which produced the predicted 440-bp and 80-bp dna fragments. a 20-mer (alpha-32p) oligonucleotide probe specifically hybridized with these amplified products confirming the specificity and reliability of this diagnostic assay. t ...19979375290
possible involvement of plasmids in degradation of malathion and chlorpyriphos by micrococcus sp.two plasmid-harboring strains of micrococcus sp. (m-36 and ag-43) degrade malathion and chlorpyriphos. derivatives of the strains (sds-36 and ao-43) treated with acridine orange and sodium dodecyl sulfate could not utilize malathion and chlorpyriphos for growth as the sole carbon source. agarose gel electrophoresis of cell extracts of m-36 and ag-43 revealed the presence of a plasmid which was absent in sds-36 and ao-43--suggesting probable involvement of plasmids in the degradation of malathion ...19979508553
histamine and tyramine degradation by food fermenting microorganisms.microorganisms suitable for food fermentation were examined with regard to their potential to degrade histamine and tyramine. out of 64 lactic acid bacteria evaluated in this study, 27 degraded histamine and one tyramine, respectively, with low activity. among 32 strains of brevibacterium linens and coryneform bacteria, 21 exhibited histamine and tyramine oxidase activity. none of 20 strains of staphylococcus carnosus tested degraded histamine or tyramine. one strain out of nine strains of geotr ...19989562873
experimental biofilm and its application in malathion degradation.mixed bacterial culture consisting of three different strains of micrococcus sp. (ag 36, ag 94 and ag 98) and two strains of pseudomonas sp. (ag 7 and ag 52) and its individual components was passed through a sand column and 25.5-92% of cell dry mass was found to be retained (adsorbed) on it. incubation of sand soaked in mineral medium containing glucose as a sole carbon source resulted in formation of a biofilm with 1.2-2.5-fold increase in biomass. a 61% degradation of malathion by the mixed c ...19989616054
production of gluconic acid using micrococcus sp.: optimisation of carbon and nitrogen sources.a process for production of gluconic acid from glucose by a micrococcus sp. is described. more than 400 bacterial cultures isolated from local soil were tested for gluconic acid production. three isolates, were selected on basis of their ability to produce gluconic acid and high titrable acidity. these were identified as micrococcus sp. and were named m 27, m 54 and m 81. nutritional and other parameters for maximum production of gluconic acid by the selected isolates were optimised. it was foun ...19969676047
in vitro synthesis of the unit that links teichoic acid to peptidoglycan.the role of cytidine diphosphate (cdp)-glycerol in gram-positive bacteria whose walls lack poly(glycerol phosphate) was investigated. membrane preparations from staphylococcus aureus h, bacillus subtilis w23, and micrococcus sp. 2102 catalyzed the incorporation of glycerol phosphate residues from radioactive cdp-glycerol into a water-soluble polymer. in toluenized cells of micrococcus sp. 2102, some of this product became linked to the wall. in each case, maximum incorporation of glycerol phosph ...1976815251
bacterial infection and endotoxin in female reproductive tract in rats: correlation with the developmental status of preimplantation embryos.during mammalian preimplantation development, a substantial numbers of embryos are believed to be lost for reasons that are unclear. using female rats, we investigated whether the developmental status of embryos is influenced by bacterial infection and endotoxin in the reproductive tract. from the vagina of cycling rats (n = 11), 21 bacterial isolates were identified; they were streptococcus faecalis (s. faecalis; 38%), escherichia coli (e. coli; 19%), acinetobactor calcoaceticus (a. calcoacetic ...19989854425
composition and antimicrobial activity of the essential oil of peumus boldus leaves.the composition and the antimicrobial activity of the essential oil from the leaves of peumus boldus is investigated. analyses of the oil obtained by hydrodistillation were carried out by gc and gc-ms using columns of two different stationary phases. fractionation of the essential oil by column chromatography on silica gel was performed to improve identification of some constituents. more than 90% of the total oil (46 components) was identified, major constituents being monoterpenes (90.5%), amo ...199910193210
[quaternary structure of histidine decarboxylase according to small-angle x-ray diffraction and electron microscopic findings].the data on small angle x-ray scattering with histidine decarboxilase (hdc) from micrococcus sp. n. were analysed and a line of succesively improving approximations of the molecule shape was found: by oblate ellipsoid a:b:c = 1:10.63, by continuous cylinder and hollow cylinder with h = 50 a, 2r = 76 a, 2r = 8a. biochemical data and electron micrographs of hdc obtained made possible to distinguish subunits and thus to increase resolution of the model. the model of the enzyme molecule consisting o ...1978698248
the linkage of sugar phosphate polymer to peptidoglycan in walls of micrococcus sp. 2102.1. protein-free walls of micrococcus sp. 2102 contain peptidoglycan, poly-(n-acetylglucosamine 1-phosphate) and small amounts of glycerol phosphate. 2. after destruction of the poly-(n-acetylglucosamine 1-phosphate) with periodate, the glycerol phosphate remains attached to the wall, but can be removed by controlled alkaline hydrolysis. the homogeneous product comprises a chain of three glycerol phosphates and an additional phosphate residue. 3. the poly-(n-acetylglucosamine 1-phosphate) is atta ...1978629757
beta-hemolytic streptococci and other beta-hemolytic organisms in apical periodontitis and severe marginal periodontitis.thirteen teeth with necrotic pulps and apical periodontitis and nine severe periodontal pockets were cultured for presence of beta-hemolytic streptococci and other beta-hemolytic organisms. samples were dispersed and plated on two non-selective and one selective growth media and incubated anaerobically and in 10% co2 in air. a total of 59 beta-hemolytic colonies were purified and identified. eight beta-hemolytic streptococcal isolates were obtained from three of the severe marginal periodontitis ...199910530151
[effect of polyakov's "prints" on the catalytic properties of an enzyme substrate system].silicagels, formed in presence of an enzyme (histidine decarboxylase from micrococcus sp. n.), substrate (l-histidine-hcl-h2o) and inhibitor (imidazole) (so-called polyakov's "print") exhibited specific catalytic properties in the reaction of l-histidine decarboxylation. the "prints" of the enzyme, inhibitor and substrate increased the activity of the enzyme-substrate system studied. the increased activity was probably due to the specific adsorption of imidazole ring of the substrate molecule by ...1975175565
bacteriology of preserved stallion semen and antibiotics in semen extenders.three experiments were conducted to evaluate the effects of different antibiotics in a milk-glucose semen extender on motility of equine sperm and elimination of bacteria following storage of extended semen in vitro. in experiment 1, 7 antibiotics were compared: amikacin, gentamicin, streptomycin, potassium penicillin, sodium penicillin, ticarcillin, and polymixin b. in experiment 2, 3 antibiotic treatments were compared: potassium penicillin g, amikacin, or a combination of potassium penicillin ...199810732147
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