| conformational and molecular responses to ph variation of the purified membrane adenosine triphosphatase of micrococcus lysodeikticus. | a preparation of atpase from the membranes of micrococcus lysodeikticus, solubilized and more than 95% pure, showed two main bands in analytical polyacrylamide gel electrophoresis. they did not correspond to isoenzymes because one band could be converted into the other by exposure to a mildly alkaline ph value. the conversion was paralleled by changes in molecular weight, circular dichroism and catalytic properties. denaturation by ph at 25 degrees c was followed by means of circular dichroism, ... | 1975 | 91 |
| membrane-reversible h+-atpase from micrococcus lysodeikticus. | treatment of phosphorylating fragments of bacterial membrane from micrococcus lysodeikticus with trypsin leads to increase atpase activity. as a result of this treatment, the membrane fragments acquire the ability to transform the atp energy into transmembrane difference in potential. dithiothreitol has a similar effect to that of trypsin on the membrane fragments from m. lysodeikticus. dicyclohexylcarbodimide inhibits atpase of the membrane fragments of m. lysodeikticus, and also the atpase-rea ... | 1976 | 4306 |
| exo-beta-n-acetylmuramidase--a novel hexosaminidase. production by bacillus subtilis b, purification and characterization. | exo-beta-n-acetylmuramidase, or beta-2-acetamido-3-o-(d-1-carboxyethyl)-2-deoxy-d-glucoside acetamidodeoxyglucohydrolase, is produced by bacillus subtilis b, growing in a succinate/peptone/salts medium, at the end of exponential growth and occurs partly in the medium and partly bound to the cells. a lysozyme digest of micrococcus lysodeikticus cell walls, o-2-acetamido-2-deoxy-beta-d-glucopyranosyl-(1 leads to 4)-2-acetamido-3-o-(d-1-carboxyethyl)-2-deoxy-d-glucose and o-2-acetamide-3-o-(d-1-car ... | 1976 | 6281 |
| isolation and partial characterization of the two major subunits of the bf1 factor (atpase) from micrococcus lysodeikticus and evidence for their glycoprotein nature. | | 1976 | 6334 |
| purification of several bacteriolytic enzymes by affinity chromatography on lysozyme-lysate of micrococcus lysodeikticus cell wall coupled with sepharose. | using lysozyme-lysate of micrococcus lysodeikticus cell wall coupled with sepharose, several bacteriolytic enzymes were purified from crude preparations of animal and microbial origin. quail egg-white, human milk and salivary lysozymes ec 3.2.1.17 were adsorbed onto the adsorbent at ph 5-7 and eluted with 2m nacl at ph 10. by means of these treatments, lysozymes were purified 20-250 fold with activity recoveries of 60-80%, and the quail lysozyme thus purified was shown to be discelectrophoretica ... | 1975 | 6436 |
| a transition state analog of lysozyme catalysis prepared from the bacterial cell wall tetrasaccharide. | treatment of the cell wall tetrasaccharide glcnacbeta(1 leads to 4)-murnac-beta(1 leads to 4)-glcnac-beta(1 leads to 4)-murnac with alkali resulted in the formation of the unsaturated tetrasaccharide glcnac-beta(1 leads to 4)-murnac-beta(1 leads to 4)-glcnac-beta(1 leads to 4)-delta2,3-2-acetamido-2-deoxy-d-glucoseen. the same compound was also formed by transglycosylation upon incubation of the unmodified tetrasaccharide with the unsaturated disaccharide glcnac-beta(1 leads to 4)-delta2,3-2-ace ... | 1976 | 6476 |
| effects of polyamines on the degradation of ribonucleic acids by polynucleotide phosphorylase of micrococcus luteus. | the effects of polyamines on the breakdown of synthetic polynucleotides poly(a), poly(c), and poly(u) by polynucleotide phosphorylase polyribonucleotide: orthophosphate nucleotidyltransferase, ec 2.7.7.8 from micrococcus luteus have been studied. although the breakdown of all the synthetic polynucleotides tested was stimulated by polyamines, the degree of stimulation by polyamines was in the order poly(c) greater than poly(a) greater than poly(u) at ph 7.5. however, the difference in degree of s ... | 1977 | 14945 |
| phosphate transport in micrococcus lysodeikticus. | phosphate accumulates in micrococcus lysodeikticus cells against a concentration gradient, by an energy-dependent process. the phosphate transport is derepressed during phosphate deprivation. the depression process is inhibited by chloramphenicol. the apparent km of phosphate transport is 4.3 micronm. the activation energy of the transport is 21 kcal per mol in the temperature range of 0-29degrees c, and 4.9 kcal per mol between 29 and 40degrees c. the rate of the transport increases in presenc ... | 1977 | 15596 |
| the enzymatic conversion of l-histidine to urocanic acid by whole cells of micrococcus luteus immobilized on carbodiimide activated carboxymethylcellulose. | whole cells of micrococcus luteus (formerly sarcina lutea atcc 9341) have been covalently linked to a carboxymethylcellulose support system, with the retention of histidine ammonia-lyase activity. the dependence of the rate of urocanic acid formation on ph, temperature, and added surfactant concentration was similar for the free and the immobilized cells. the immobilization procedure used is based on the carbodiimide activation of carboxymethylcellulose and has been optimized for the histidine a ... | 1977 | 15677 |
| the testing of the antibiotic sensitivity of bacteria on an agar medium: the problem of a double zone of inhibition. | when the sensitivity of micrococcus luteus attc9341 to streptomycin, erythromycin, oleandomycin and spiramycin was tested by an agar diffusion method using antibiotic impregnated filter paper disks on unbuffered penassay seed agar two zones of inhibition were observed around the disks after an incubation period of 24 hours at 30 degrees c. the ph of the m. luteus seeded penassay seed agar was measured before and after 24 hours incubation at 30 degrees c and found to be 6.6 and 8.7, respectively. ... | 1977 | 16428 |
| alpha-adrenergic blocking action of fentanyl on the isolated aorta of the rabbit. | the contractile response of helically-cut strips of rabbit ascending aorta to transmural electrical stimulation was attenuated in a dose-dependent manner by treatment for 20 min with fentanyl, 10(-6) to 10(-5) m. fentanyl also shifted the dose-response curve of the contractile response of aorta to norepinephrine to the right. the response to transmural stimulation was more resistant to fentanyl than was the response to an equipotent dose of norepinephrine. the inhibitory effect of fentanyl was ... | 1977 | 16512 |
| diagnosis of equine crytorchidism. | | 1977 | 17211 |
| phosphonic analogues as antagonists of amino acid excitants. | | 1977 | 17707 |
| bacteriorhodopsin-mediated photophosphorylation in halobacterium halobium. | the rate of halobacterial photophosphorylation was found to be a linear function of light intensity over a wide range (between 1 and 20 mw/cm2). at higher light intensities (above 25 mw/cm2) the atp-synthesizing system itself limits the maximal rate of photophosphorylation. the optimal external ph range for this type of photophosphorylation is between ph 6.2 and 7.2 external. the photophosphorylation rate is directly proportional to the bacteriorhodopsin content of the cells. the quantum require ... | 1977 | 19249 |
| influence of solute and hydrogen ion concentration on the water relations of some xerophilic fungi. | germination and growth of six xerophilic fungi, aspergillus flavus, aspergillus ochraceus, eurotium chevalieri, chrysosphorium fastidium, wallemia sebi and xeromyces bisporus were examined on media of a wide range of water activities (aw). the influence of three solutes, nacl, glycerol and a glucose/fructose mixture, was studied at ph 4-0 and ph 6-5 using a plate-slide technique. germination times and growth rates were affected by solute type, but the influence of ph was less marked. except for ... | 1977 | 19558 |
| analysis of the activity of micrococcus luteus endonucleases with respect to gamma-irradiated dna. | endonucleases from micrococcus luteus that induce single-strand breaks in gamma-irradiated dna have been separated chromatographycally into two groups. the first group involves two different enzymes: ap-endonuclease ii (mol. weight 30 000) and ap, uv-endonuclease i (mol. weight 15 000) that recognize alkali-labile lesions in gamma-irradiated dna and apurinic sites in dna heated at 70 degrees c, ph 6.08 ap-endonuclease ii in cooperation with dna polymerase from m. luteus and t4 phage-induced poly ... | 1977 | 20161 |
| monoamine metabolite levels in cerebrospinal fluid of psychotic women treated with melperone or thiothixene. | psychotic women with schizophrenic symptoms were treated with melperone 100 mg x 3 (n = 29) or thiothixene 10 mg x 3 (n = 34) using a double-blind procedure. before and during treatment, levels of hva, mopeg, and 5-hiaa, the major metabolites of da, ne, and 5-ht, were determined in lumbar cerebrospinal fluid by a mass fragmentographic technique. both treatments resulted in an elevation of the hva levels after 2 weeks, thiothixene having a more marked effect. the effect of thiothixene but not of ... | 1977 | 21644 |
| relationship between intramitochondrial citrate and the activity of carbamoyl-phosphate synthase (ammonia). | the possibility of control of the activity of carbamoyl-phosphate synthase (ammonia) (ec 2.7.2.5) in rat-liver mitochondria by variation in the intramitochondrial free mg2+ concentration has been investigated. carbamoyl-phosphate synthase activity was measured by coupling the formation of carbamoylphosphate to the synthesis of citrulline in a reaction mixture containing ammonia, bicarbonate, a source of atp, and ornithine. the synthesis of citrulline was inhibited by lowering the concentration o ... | 1977 | 21702 |
| the effect of ionophores on phosphate and arsenate transport in micrococcus lysodeikticus. | | 1977 | 21813 |
| in vivo oxidation of reduced nicotinamide-adenine dinucleotide phosphate by paraquat and diquat in rat lung. | intravenous injection of rats with 156 mumol/kg of paraquat or 140 mumol/kg of diquat produced, within 60 min, a sharp drop in the ratios of nadph to nadp in lung. the effect persisted for a time period of at least 24 h. exposure to 100% oxygen enhanced the toxicity of both compounds without substantially amplifying changes in the nadph/nadp ratio. lungs retained the capability to synthesize adenine nucleotides de novo. electron microscopic studies showed that both paraquat and diquat damage typ ... | 1977 | 22404 |
| mutagenic and recombinogenic action of pesticides in aspergillus nidulans. | thirteen pesticides, aminotriazole, benomyl, captafol, captan, dalapon-na, dichlorvos, dinobuton, dodine, ioxynil, mecoprop, neburon, picloram and tordon were tested for ability to induce (1) point mutations to 8-azaguanine resistance, (2) mitotic crossing-over, and (3) mitotic non-disjunction and haploidization in aspergillus nidulans. tests were performed at three different phs, i.e. 4.5, 7, 8.2. three of the pesticides, captan , captafol and dichlorvos induced point mutations; dichlorvos also ... | 1977 | 22812 |
| methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase from porcine liver. interaction between the dehydrogenase and cyclohydrolase activities of the multifunctional enzyme. | methylenetetrahydrofolate dehydrogenase (5,10-methylenetetrahydrofolate: nadp+ oxidoreductase, ec 1.5.1.5) one of the activities of a trifunctional folate-dependent enzyme from porcine liver, uses an ordered kinetic mechannism as determined from initial velocity, product inhibition and dead-end inhibition studies. the final product released from the dehydrogenase is methenyltetrahydrofolate. however, from the time course of appearance of products it is observed that the methenyltetrahydrofolate, ... | 1978 | 23838 |
| detection and prevalence of pneumococci with increased resistance to penicillin. | susceptibility to penicillin was determined for 6000 strains of pneumococci isolated during 1974--76 from patients in alberta and the adjacent region of the northwest territories. strains were considered to be relatively resistant if the minimum inhibitory concentration (mic) of penicillin was 0.16 microgram (0.26 u)/ml or more, which is eight or more times greater than the mic for fully susceptible strains. resistance was detected in 143 strains (2.4%) isolated from 122 patients and belonging t ... | 1977 | 23894 |
| cardiovascular responses to nitrous oxide during light, moderate, and deep halothane anesthesia in man. | | 1978 | 24374 |
| active groups of extracellular endo-d-galacturonanase of aspergillus niger derived from ph effect on kinetic data. | in an attempt to characterize the groups essential for the catalytic action extracellular endo-d-galacturonanase of aspergillus niger (poly (1,4-alpha-d-galacturonide) glycanohydrolase, ec 3.2.1.15) the behaviour of the kinetic parameters as a function of ph was examined. the dependence of kcat and kcat/km on ph suggests that two dissociable groups are involved, for which the pk values of about 3.0 and 5.0 in the free enzyme and 3.06 and 5.72 in the catalytic complex were found at 30 degrees c. ... | 1978 | 24473 |
| purification and characterization of lysozyme produced by streptomyces erythraeus. | a species of lysozyme (se lysozyme) was purified from culture filtrate of streptomyces erythraeus. the enzyme has a molecular weight of 18,500 as determined by ultracentrifugation. its isoelectric point is 9.5, and it shows optimal activity at ph 4.0 with an optimal ionic strength of 0.1. investigation of the substrate specificity showed se lysozyme to be an n-acetyl-muramidase. the simplest product in the digest of cell walls of micrococcus lysodeikticus was identified as a disaccharide, glcnac ... | 1978 | 25274 |
| biochemical aspects of cardiac muscle differentiation. | experiments were designed to determine whether dna synthesis ceases in terminally differentiating cardiac muscle of the rat because the activity of the putative replicative dna polymerase (dna polymerase alpha) is lost or whether the activity of this enzyme is lost because dna synthesis ceases. dna-template availability and 3'-hydroxyl termini in nuclei and chromatin, isolated from cardiac muscle at various times during the developmental period in which dna synthesis and the activity of dna poly ... | 1978 | 26332 |
| cell wall autolysis in log phase cells of micrococcus lysodeikticus (luteus). | log phase cells of micrococcus lysodeikticus (luteus) ifo 3333 autolyzed when incubated at 37 c in 0.01 m sodium-phosphate buffer ph 7.5. the enzyme involved in the autolysis was recovered mainly in an aqueous phase from cytoplasmic membranes and cytoplasmic materials treated with n-butanol, and proved to be an n-acetylmuramyl-l-alanine amidase. the autolysis of log phase cells suspended in autolyzing buffer was depressed by the addition of trypsin to the buffer. | 1978 | 27703 |
| an experimental model for osteomyelitis in the mandible. | | 1977 | 28338 |
| hr 756, the syn isomer of a new methoxyimino cephalosporin with unusual antibacterial activity. | hr 756, the syn derivative of 7-[(2-(2-amino-4-thiazolyl)-2-methoxyimino)acetamido]cephalosporanic acid, is a new semisynthetic cephalosporin. it was 80 times more active than the anti derivative against beta-lactamase-producing strains of gram-negative bacteria. the range of inhibitory concentrations of hr 756 against gram-negative bacteria, including haemophilus influenzae, susceptible or resistant to penicillins and cephalosporins was from 0.01 to 0.1 mug/ml. this activity was consistently hi ... | 1978 | 31836 |
| purification and properties of rabbit alveolar macrophage lysozyme. | lysozyme was isolated from bacillus calmette-guerin-elicited rabbit alveolar macrophages by acid extraction and purified to homogeneity by a single-column procedure. yields of the purified enzyme averaged between 20 and 30 mg per rabbit, values far in excess of those obtained with previously published methods. rabbit lysozyme has a molecular weight of 14,300 and exhibits optimal lytic activity against micrococcus lysodeikticus at an ionic strength of 0.04, ph 6.5. our results indicate that lysoz ... | 1979 | 37167 |
| determination of virginiamycin in feeds. | virginiamycin was extracted from the feed by ethanol-ph 2.5 phosphate buffer (1 + 1). the ph during extraction was adjusted (when necessary) to between 4 and 5. sample dilutions and the standard dose response line were prepared to contain ethanol ph 6 phosphate buffer (2 + 8), and the test organism was sarcina lutea. three feeds (a poultry ration, a swine finishing ration, and a swine starter ration) showed virginiamycin recovery of 88.8--108.9% when standard solutions were added at concentratio ... | 1979 | 39060 |
| biochemical properties of penicillin amidohydrolase from micrococcus luteus. | some biochemical properties of whole-cell penicillin amidohydrolase from micrococcus luteus have been studied. this whole-cell enzyme showed its maximal activity at 36 degrees c at ph 7.5. it was found that the activation energy of this enzyme was 8.03 kcal (ca. 33.6 kj) per mol, and this amidohydrolase showed first-order decay at 36 degrees c. the penicillin amidohydrolase was deactivated rapidly at temperatures above 50 degrees c during storage or preincubation for 24 h. the michaelis constant ... | 1979 | 39505 |
| characterization of the autolytic enzymes of clostridium perfringens. | clostridium perfringens and isolated walls of this organism autolysed rapidly when incubated in buffer at ph 7.0 with the release of free-reducing groups but no n-terminal amino acids. the predominant autolytic enzyme was an endo-beta-n-acetylglucosaminidase, and an endo-beta-n-acetylmuramidase was also present. the autolytic enzymes could be solubilized by extraction of the organisms with 5 m-licl and would then subsequently bind to and rapidly lyse walls of micrococcus luteus and, more slowly, ... | 1979 | 44314 |
| isolation and characterization of homogenous rabbit antibodies to micrococcus lysodeikticus with specificity to the peptidoglycan and to the glucose-n-acetylaminomannuronic acid polymer. | the antibody response of rabbits to micrococcus lysodeikticus is characterized by the production of a high concentration of antibodies which manifest markedly reduced heterogenicity. the specificity of these antibodies was studied and it revealed that m. lysodeikticus contains 2 major antigens: both the glucose-n-acetyl-aminomannuronic acid polymer obtained by formamide extraction of the cell walls and peptidoglycan solubilized by ultrasonic treatment gave precipitin reactions with hyperimmune a ... | 1975 | 52238 |
| mode of inhibition of herpes simplex virus dna polymerase by phosphonoacetate. | phosphonoacetate is a highly specific inhibitor of herpes simplex virus-induced dna polymerase. sensitivity of herpesvirus type 1 or type 2 induced dna polymerase to the drug was similar. however, dna polymerases from other sources such as the host cells (wi-38), micrococcus luteus, and hepatitis b virus were highly resistant. in addition, escherichia coli rna polymerase and reverse transcriptase of rous sarcoma virus were also insensitive to the drug. enzyme kinetic studies showed that inhibiti ... | 1975 | 53071 |
| [change in lipid-protein interactions in the membranes of bacteria exposed to gramicidin s]. | effect of cyclopeptide antibiotic gramicidin s on some enzymes and physical state of isolated micrococcus lysodeikticus membranes is studied. malate and lactate dehydrogenases were monotonously inhibited under the increase of gramicidin s concentration, while the activity of nadh-dehydrogenase firstly decreased and then reversed to the initial level under further increase of gramicidin s concentration. the oxygen uptake under oxidation of nadh and malate with membranes almost completely inhibite ... | 1976 | 58672 |
| chalcosis in the human eye. a clinicopathologic study. | a clinicopathologic study of eight human eyes containing intraocular copper foreign bodies was undertaken. these foreign bodies, which had been retained in the eyes from three weeks to 22 years, were analyzed by atomic-absorption spectrophotometry and x-ray diffraction to determine their chemical composition. copper deposits in descemet membrane, vitreous, internal limiting membrane of the retina, and fibrous capsule around the foreign bodies could be demonstrated by rhodanine, rubeanic acid, an ... | 1976 | 60098 |
| the action of local anaesthetics on histamine release. | | 1976 | 60105 |
| immunochemical study of the peptidoglycan of gram-negative bacteria. | the specificity of antibodies directed against the peptidoglycan of gram-negative bacteria was studied. the peptidoglycans of proteus vulgaris, escherichia coli, moraxella glucidolytica, neisseria perflava, give identical precipitin reactions. by means of inhibition studies with various peptidoglycan subunits and synthetic peptides, it was shown that the antibodies are essentially directed against the peptide moiety of the peptidoglycan: l-ala-d-glu (l)-mesoa2pm-(l)-d-ala, that the peptide react ... | 1976 | 60233 |
| radioimmunoassay of neocarzinostatin, an antitumor protein. | antibodies directed toward the antitumor protein neocarzinostatin (ncs) have been produced in a rabbit by immunization with a highly purified ncs preparation. the antiserum was monospecific and reversed the antibacterial activity of ncs against sarcina lutea. it cross-reacted with chemically modified derivatives of ncs and mitomalcin but failed to cross-react with macromomycin. a radioimmunoassay procedure has been developed utilizing the antiserum and a biologically active 125i-labeled derivati ... | 1976 | 63321 |
| the immune response to micrococcus lysodeikticus. 1. evolution of the response in two litter mates. | | 1976 | 64217 |
| restriction of the anti-bovine serum albumin response in rabbits immunized with micrococcus lysodeikticus. | rabbits capable of producing antibodies of restricted heterogeneity in response to micrococcus lysodeikticus are equally capable of producing antibodies of restricted heterogeneity to bovine serum albumin. these antibodies are produced when animals are simultaneously injected with micrococcus and bsa and their specificity is restricted to a small number of epitopes. these results suggest that micrococcal vaccines can induce the restriction of heterogeneity in antibodies raised against totally un ... | 1977 | 71263 |
| comparative immunological study of catalases in the genus micrococcus. | double immunodiffusion tests were performed with crude extracts from various micrococcus species and antisera against catalase of micrococcus luteus ccm 169. cell-free extracts of m. lylae atcc 27566 exhibited good cross-reaction. cell-free extracts or catalase enriched preparations of m. varians reacted very weakly and no reaction has been found with preparation of m. kristinae, m. nishinomiyaensis, m. roseus and m. sedentarius. the quantitative microcomplement fixation assay also revealed a cl ... | 1977 | 71880 |
| immunological properties of membrane-bound adenosine triphosphatase: immunological identification of rutamycin-sensitive f0.f1atpase from micrococcus luteus atcc 4698 established by crossed immunoelectrophoresis. | (1) f0.f1atpase (ec 3.6.1.3) from micrococcus luteus atcc 4698 was solubilized from plasma membranes by the non-ionic detergent triton x-100 in the presence of 0.05 m mgcl2. (2) the antibiotics rutamycin, dio-9, quercetin, oligomycin, botrycidin, efrapeptin, leucinostatin, valinomycin, and venturicidin as well as n,n'-dicyclohexylcarbodiimide and dinitrophenol are potent inhibitors of f0.f1atpase activity.(3) f0.f1atpase activity is completely inhibited by anti-f1atpase antibodies. the inhibitio ... | 1978 | 77681 |
| non-specific tumour immunotherapy by micrococcus lysodeikticus and related polysaccharides [proceedings]. | | 1977 | 79358 |
| polypeptide antibiotic 26a from bacillus subtilis. ii. isolation and purification procedures. | both the analytical and preparative methods by which the preparations of 26a bydrochloride salt with a high antibacterial activity and 20--30% recovery have been obtained from the fermentation fluids of bacillus subtilis are presented. on an industrial scale the antibiotic can be yielded by absorption of bioactivity on amberlite cg-50i column and precipitation with picric acid of crude substance from active elutes as adduct which was divided on equilibrated cm--cellulose and finally purified by ... | 1978 | 81596 |
| reactions of chicken antibodies with immunoglobulins of mouse serum and t cells. | | 1978 | 83961 |
| human breast carcinoma antigen is immunologically related to the polypeptide of the group-specific glycoprotein of mouse mammary tumor virus. | we have shown [mesa-tejada, r., keydar, i., ramanarayanan, m., ohno, t., fenoglio, c. & spiegelman, s. (1978) proc. natl. acad. sci. usa 75, 1529--1533] that an antigen immunologically related to gp52, a 52,000-dalton glycoprotein of the mouse mammary tumor virus, can be identified in sections of human breast cancer by means of an indirect immunoperoxidase technique. the specificity of the reaction was established by absorption experiments which revealed that only purified gp52, or material cont ... | 1979 | 88056 |
| passive transfer in diabetes mellitus. | | 1979 | 88629 |
| a new assay of estrogen receptor by thin-layer gel filtration. | | 1979 | 88911 |
| [compartmentation of gramicidin 5 in membranes of sensitive bacteria and protein-lipid interactions]. | gramicidin s is sorbed on the isolated membranes of granicidin-sensitive micrococcus lysodeikticus strain. the antibiotic inhibits the membrane malate dehydrogenase within the temperature range of 9--42 degrees c, i.e. under conditions of gel and liquid-crystalline lipid state; however its effect at 10 degrees c is 10 times as low as is observed at 42 degrees c. the inhibitory effect of gramicidin s on malate dehydrogenase can be eliminated and the antibiotic can be removed from the membrane by ... | 1979 | 88965 |
| [heterogeneity in organization of lipids in the bacterial membrane]. | the lipids of micrococcus lysodeikticus membranes were 50%-substituted by phosphatidyl choline using lipid-exchanging proteins isolated from rat liver. the incorporation of phosphatidyl choline into the membrane did not significantly change the malate dehydrogenase activity and the temperature dependence activity in the arrhenius plots for the enzyme. gramicidin s--modifier of membrane lipids--had similar effects both on the intact membranes and on the phosphatidyl-enriched membranes. a conclusi ... | 1979 | 88966 |
| the early effects of chemical carcinogens on adult rat hepatocytes in primary culture. ii. effects on unscheduled dna synthesis, cell division and alpha-fetoprotein production. | the effects of exposure of rat hepatocytes in primary maintenance culture to chemical carcinogens has been studied with respect cytotoxicity and alterations in mitotic index, unscheduled dna synthesis and alpha-fetoprotein (afp) production. all compounds tested produced cytotoxicity. increases in mitotic index and unscheduled dna synthesis and the production of afp were observed after treatment of the cells with the carcinogens but not after treatment with the non-carcinogenic isomers. these inc ... | 1979 | 89011 |
| cyclic gmp system in epidermis: i. effect of ischemia. | when keratome-sliced pig epidermis was floated on hank's balanced salt solution, we observed a rapid decrease in the intracellular level of cyclic gmp. a portion of the lost cyclic gmp was detected in the incubation medium. when the epidermis was kept in air at room temperature, the cyclic gmp level also decreased rapidly but to a lesser degree. incubating the epidermal slice at 37 degrees c in hank's balanced salt solution with the addition of 3-isobutyl-1-methyl xanthine (ibmx) prevented the d ... | 1979 | 89173 |
| simulation and prevention of retrovirus--specific reactions by mycoplasmas. | from human mycosis fungoides tumor-derived cell lines, mycoplasma hyorhinis was isolated. this mycoplasma shared the following characteristics with retroviruses: uptake of 3h-uridine, but not of 3h-thymidine in cell culture; banding at 1.16 g/ml sucrose density and partial shift to retrovirus core density position (approximately equal to 1.24 g/ml) after detergent treatment; incorporation of 3h-tmp into high molecular weight material in standard reverse transcriptase assays with the template-pri ... | 1979 | 89623 |
| electron microscopic study of the rat cerebral cortex, impregnated by the golgi method. | | 1978 | 89643 |
| pharmacological models for characterizing new anti-asthmatics. | | 1978 | 89745 |
| histological diagnosis of myocardial injury. comparison of hematoxylin-basic fuchsin-picric acid (hbfp)-stained sections obtained during autopsy with isolated viable rat cardiac myocytes exposed to anoxia. | the light microscopic appearances in hematoxylin-basic fuchsin-picric acid (hbfp)-stained histological sections from cardiac and skeletal muscle tissue were put in relation to the reactions of isolated viable rat cardiac myocytes exposed to anoxia in suspension and their morphology in paraffin-embedded sections. special attention was paid to prenecrotic phases of myocytic injury which were followed, in viable rat cardiac myocytes, by light microscopy, and confirmed with biochemical assays indica ... | 1979 | 89781 |
| in vitro erythropoiesis. ii. cytochemical enumeration of erythroid stem cells (cfu-e and bfu-e) from normal mouse and human hematopoietic tissues. | a procedure which provides more accuracy in the identification and scoring of in vitro derived erythroid colonies (i.e. bfu-e and cfu-e) from normal mouse and human marrow and spleen cells has been developed. up to now identification of erythroid colonies has been based on staining with acidified benzidine which consistently displays "background staining" of granulocyte/macrophage colonies. the staining reaction with these reagents is such that identification of erythroid colonies is less than p ... | 1979 | 89963 |
| toxoplasma gondii: ultrastructure and antigenicity of purified tachyzoite pellicle. | | 1979 | 89966 |
| ribosomal ambiguity (ram) mutations facilitate diyhydrostreptomycin binding to ribosomes. | | 1979 | 89972 |
| human blood t lymphocytes that suppress the mixed leukocyte culture reactivity of lymphocytes from hla-b14 bearing individuals. | a nontransfused male patient with recurrent bladder carcinomata has been demonstrated to have blood t lymphocytes that suppress the mlc responsiveness of lymphocytes only from normal individuals positive for hla-b14. unexpectedly, this t-suppressor cell differs from three other reported blood t-suppressor cells arising in man in that it apparently does not require hla-d locus compatibility between the suppressor cell and the lymphocyte being suppressed. | 1979 | 90092 |
| characterization of a monoclonal antibody directed against mouse macrophage and lymphocyte fc receptors. | to investigate the antigenic relationship between the macrophage and lymphocyte fc receptors (fcr), a monoclonal antibody capable of blocking mouse macrophage fc receptors was selected. hybrids were formed by fusing the p3u1 mouse myeloma and spleen cells from a rat immunized with the mouse macrophage-like cell lines j774 and p388d1. the fab fragment of the monoclonal igg secreted by clone 2.4g2, inhibited the trypsin-resistant fc receptor ii (fcrii), which is specific for immune aggregates of m ... | 1979 | 90108 |
| bites by the carpet viper in the niger valley. | 60 people who had been bitten by the carpet viper, echis carinatus, were seen in four months at zungeru health centre, in north west nigeria, where snake-bite patients have recently occupied 10% of beds. health statistics greatly underestimate the frequency, morbidity, and mortality of snake bite in the rural tropics and further studies will probably confirm other areas endemic for e. carinatus within its vast distribution zone. regular supplies of effective antivenom are crucial to gain a commu ... | 1979 | 90281 |
| [lysis of micrococcus lysodeikticus protoplasts by gramicidin s derivatives]. | gramicidin s derivatives with the substituted amino groups of ornithines (carbamoylgramicidin and diacetylgramicidin) possess nearly the same level of the lytic activity when acting on micrococcus lysodeikticus protoplasts in a 1 m sucrose solution as the original antibiotic. the lytic activity of gramicidin s and these derivatives considerably increases in a solution of sucrose in phosphate buffer. the dependence of the lytic activity of gramicidin on its concentration is of a complex character ... | 1979 | 90326 |
| [effect of organochlorine pesticides on the ocular electric response of the white mouse]. | the injection of ddt or hch resulted in a decrease of the amplitude of b-wave of electroretinograms without any modification of its latency, whereas a-wave remained unchanged. in conclusion, we may say that organochlorous pesticides do not act on photoreceptors or synapses, but they act only on bipolar and ganglionnar neurones. | 1979 | 90547 |
| cell-free synthesis and membrane insertion of mouse h-2dd histocompatibility antigen and beta 2-microglobulin. | messenger rna from sl2 lymphoma cells was translated in a cell-free system in the presence of microsomal membranes. mouse h-2dd histocompatibility antigen was correctly assembled in the microsomal membranes, and transmembrane insertion of the nascent chain was accompanied by glycosylation and cleavage of the signal sequence h-2kd antigens, synthesized in vivo, comprised a transmembrane glycoprotein and an unglycosylated protein in the cytoplasm. the glycosylated forms of the h-2dd and h-2kd anti ... | 1979 | 90552 |
| alpha 2 micro-globulin in the rat. the regulation of the appearance of multiple forms in vivo and in primary cultures of adult hepatocytes. | | 1979 | 90675 |
| protection of rhesus monkeys from lassa virus by immunisation with closely related arenavirus. | | 1979 | 90819 |
| abnormal levels of antibodies to measles-virus proteins in patient with mental retardation and seizures 24 years after measles encephalitis. | | 1979 | 91066 |
| purification of antilymphocyte antibody (ala) from patients with systemic lupus erythematosus (sle)-immunoabsorption and elution. | purification of antilymphocyte antibody (ala) from patients with systemic lupus erythematosus (sle) was achieved by immunoabsorption and elution. human tonsil cells or thymocytes were used as absorbents. complement dependent microcytotoxicity tests showed that, in comparison to the parent sera, the eluate from tonsil cells was eight times, and that from thymocytes four times, more active. antinuclear activity was eliminated by elution. the ala was almost entirely igm, igg being involved in only ... | 1979 | 91309 |
| the specificity of human autoantibodies that react with both cell nuclei and plasma membranes: the nuclear antigen is present on core mononucleosomes. | we have examined the nature of the nuclear antigen recognized by certain natural human antibodies that react specifically with both cell nuclei and plasma membranes from many species. partial purification of these antibodies, called x-ana, is achieved by binding to and rapid elution from the surface of viable human leukocytes. chicken erythrocyte chromatin was solubilized by digestion with staphylococcal nuclease and fractionated into a 0.15 m nacl soluble fraction that consisted of core mononuc ... | 1979 | 91640 |
| importance of the neural predilection of mycobacterium leprae in leprosy. | it is suggested that continuous leakage of bacilli into the circulation from a primary focus of intraneural infection may simultaneously initiate bacillary dissemination and the suppression of cell-mediated immunity. both these features are essential for the development of lepromatous leprosy. nerve involvement in leprosy, previously thought of as a diagnostic feature of the disease and as a complication of therapy, may represent an essential phase in the cycle of infection and reinfection by my ... | 1979 | 91779 |
| [the effect of cerebral perfusion pressure in cerebral infarction after occlusion of middle cerebral artery in dog (author's transl)]. | | 1979 | 91991 |
| new types of escherichia coli k-12 facultative recombination deficient mutants resistant to ultraviolet. | numerous facultative temperature sensitive recombination deficient mutants of escherichia coli k-12 strain 108 were isolated after mutagenization with nitrosoguanidine. the majority of the mutants were resistant to uv irradiation. three mutants, kbp72, kbp169 and kbp610, with marked recombination deificiency (300 to 15,000 times) at 42 degrees c, were uv resistant; their sensitivity to mitomycin c was altered only slightly or not at all. mutation kbp72 was co-transduced with ilv (83 unit on e. c ... | 1979 | 92170 |
| failure of corticosteroids to influence three enzymes involved in neurotransmitter metabolism in adult rat brain [proceedings]. | | 1979 | 92255 |
| role of tryptophan residue 62 in the refolding of hen egg-white lysozyme [proceedings]. | | 1979 | 92273 |
| cytochemical detection of peroxisomal oxidases. | | 1979 | 92494 |
| benign prostatic hyperplasia in a squirrel monkey (saimiri sciureus). | benign prostatic hyperplasia was diagnosed at necropsy in a middle-aged squirrel monkey (saimiri sciureus). grossly, the prostate gland was asymmetrically enlarged and had an irregular surface. microscopically, both cystic glandular hyperplasia and fibromuscular, nodular hyperplasia were identified. | 1979 | 92596 |
| changes of basic biochemical indices in rat liver tissue after intraperitoneal application of endotoxin. | | 1979 | 92874 |
| penicillin and cephalosporin immunogenicity in man. | whether cross-allergenicity exists between the penicillin and cephalosporin antibiotics is controversial. a solid-phase radioimmunoassay was adapted to detect drug-specific igg, igm and ige antibodies. penicillin and cephalothin-specific serum immunoglobulins were determined in patients' serum before and after a therapeutic course of sodium cephalothin. changes in igg, igm and ige antipenicillin and anticephalothin antibodies, respectively, occurred and were closely correlated to one another (r ... | 1979 | 92902 |
| binding of bursal, thymic and splenic lymphocytes to macrophages. | | 1979 | 93085 |
| new look at muscular dystrophy. | | 1979 | 93213 |
| antigenic similarities between human plasma inhibitor of thrombin (at-iii) and its analogues from other mammals. | cross reactions investigated by double immunodiffusion and by immunoelectrophoresis between human plasma and plasma of 5 animal species using a specific rabbit antiserum against human at-iii revealed antigenic relationships between the antithrombins of these species. but the quantitative measurements of at-iii by immunologic methods (electro-immunodiffusion and radial immunodiffusion), using the rabbit antiserum, were not possible in common laboratory animals, except in baboons. | 1979 | 93495 |
| recognition of protozoan parasite antigens by murine t lymphocytes. i. induction of specific t lymphocyte-dependent proliferative response to leishmania tropica. | | 1979 | 93546 |
| immunochemical studies on alcoholic hyalin. | | 1979 | 93559 |
| partial amino acid sequences of rabbit and rat beta 2-microglobulins. | | 1979 | 93696 |
| an approach to tracing neuron networks in the cerebral cortex and basal ganglia. combination of golgi staining, retrograde transport of horseradish peroxidase and anterograde degeneration of synaptic boutons in the same material. | | 1979 | 93712 |
| effects of intestinal amylase and trypsin on pancreatic secretion in the pig. | pigs were surgically prepared with external pancreatic fistulae and duodenal cannulae in order to elucidate whether the proposed intestinal feedback control of pancreatic secretion in the pig--as in rat and man--is exerted by trypsin. furthermore, the effect of intraluminal amylase on pancreatic secretion was studied. reintroduction of pancreatic juice into the duodenum or infusion of trypsin into the duodenum depressed the volume of the pancreatic flow and the protein output markedly. introduct ... | 1979 | 93774 |
| an in vitro study on the effect of benzoyl peroxide 20%, gentamicin and phenoxyethanol on staphylococcus aureus, pseudomonas aeruginosa and proteus species. | | 1979 | 94224 |
| [histochemical studies on the structure and the age changes of descemet's membrane in cattle (author's transl)]. | descemet's membrane of 15 cattle-corneas, aged 6 month to 13 years was studied by various histochemical and morphometric methods. 3 zones could be differentiated. zone 1 is relatively small and does not show significant changes with age; zone 2 comprises the main part of descemet's membrane, its diameter increased with age. zone 2 is rich in sulfated glycosaminoglycans. zone 3, below the endothelial lining, does not develop before the 3rd and 5th year of life and stains different from zone 2. | 1979 | 94246 |
| priming increases the amount of interferon mrna in poly(ri).poly(rc)-treated l cells. | priming by mouse interferon pre-treatment resulted in an accumulation of interferon mrna in poly(ri).poly(rc)-treated l cells, starting early in the period of interferon synthesis. on electrophoresis, the priming activity of an interferon preparation co-migrated with the antiviral activity, which suggests identity of the functional principle(s) for these activities. | 1979 | 94345 |
| effects of etafenone and antiarrhythmic drugs on na and ca channels of guinea pig atrial muscle. | since ca-mediated electrophysiological phenomena seem to be important in the generation of various arrhythmias, we quantitatively compared the effects of quinidine, disopyramide, procainamide, lidocaine, propranolol, and etafenone, a new coronary vasodilator with antiarrhythmic properties, on na and ca channels in normal and depolarized guinea pig atria. the maximum rate of rise of the normal action potential and the amplitude of the depolarized, ca-mediated action potential were measured to exa ... | 1979 | 94412 |
| studies on the nature of the type specific antigen of erysipelothrix rhusiopathiae. | | 1979 | 94508 |
| [study of membrane proteins from microccus lysodeikticus using immunochemical methods]. | using immunoelectrophoresis, the antigenicity of various protein fractions of the micrococcus lysodeikticus membranes was evaluated. it was shown that both the peripheral and integral membrane proteins possess the antigenic determinants. the antiserum exhausted by the m. lysodeikticus mebranes loses its ability to interact with intergral proteins, which are not solubilized by triton x-100. it was thus assumed that the integral proteins are exposed on the membrane surface constantly or periodical ... | 1979 | 94839 |
| inhibition and stimulation of histamine release by oxatomide. | | 1979 | 95250 |
| plasma amine oxidase activity in 136 normal rhesus monkeys: relationships between enzyme activity and age, sex and genetic factors. | normative data on plasma amine oxidase activity were obtained for four primate species, m. mulatta, m. arctoides, c. aethiops and h. sapiens. significant between-species differences in activity were observed. in a large sample of m. mulatta, no significant relationship between age and plasma amine oxidase activity was observed, but adult males had significantly higher enzyme activities than adult females. | 1977 | 96261 |
| prolactin and tsh responses to trh, chlorpromazine and l-dopa in children with human growth hormone deficiency. | prolactin (prl) and tsh responses to trh, chlorpromazine (cpz) and l-dopa were studied in 23 children (15 male and 8 female) with human growth hormone (hgh) deficiency. eight patients (group i) showed normal prl response to trh and cpz but tsh response to trh was delayed in 4 of this group. twelve patients (group ii) had normal (4 patients) or higher (8 patients) baseline prl level and showed lower prl response to cpz than that to trh. tsh response to trh was normal in 3, blunted in 1, and delay ... | 1978 | 96645 |
| unpredictable response of pseudomonas aeruginosa to synergistic antibiotic combinations in vitro. | the combined activity of ticarcillin and each of three aminoglycosides, gentamicin, tobramycin, and amikacin, was studied with 45 clinical isolates of pseudomonas aeruginosa. all strains individually were susceptible to each of these agents. comparison of the results revealed highly variable interaction, both from strain to strain and from one aminoglycoside to another against the same strain. against 11 strains, none of three antibiotic pairs tested was synergistic by conventional criteria; aga ... | 1978 | 96736 |