| poly(8-aminoguanylic acid): formation of ordered self-structures and interaction with poly(cytidylic acid). | poly(8-aminoguanylic acid) has in neutral solution a novel ordered structure of high stability. the 8-amino group permits formation of three hydrogen bonds between two residues along the "top", or long axis, of the purines. the usual hydrogen bonding protons and watson-crick pairing sites are not involved in the association. the bonding scheme has a twofold rotation axis and is hemiprotonated at n(7). poly(8nh2g) is converted by alkaline titration (pk = 9.7) to a quite different ordered structur ... | 1975 | 37 |
| modification of arginine and lysine in proteins with 2,4-pentanedione. | primary amines react with 2,4-pentanedione at ph 6-9 to form enamines, n-alkyl-4-amino-3-penten-2-ones. the latter compounds readily regenerate the primary amine at low ph or on treatment with hydroxylamine. guanidine and substituted guanidines react with 2,4-pentanedione to form n-substituted 2-amino-4,6-dimethylpyrimidines at a rate which is lower by at least a factor of 20 than the rate of reaction of 2,4-pentanedione with primary amines. selective modification of lysine and arginine side cha ... | 1975 | 43 |
| conformational and molecular responses to ph variation of the purified membrane adenosine triphosphatase of micrococcus lysodeikticus. | a preparation of atpase from the membranes of micrococcus lysodeikticus, solubilized and more than 95% pure, showed two main bands in analytical polyacrylamide gel electrophoresis. they did not correspond to isoenzymes because one band could be converted into the other by exposure to a mildly alkaline ph value. the conversion was paralleled by changes in molecular weight, circular dichroism and catalytic properties. denaturation by ph at 25 degrees c was followed by means of circular dichroism, ... | 1975 | 91 |
| flourescent properties of histidine decarboxylase from micrococcus sp. n. | a dependency of fluorescence parameters of histidinedecarboxylase (hdc) from micrococcuc sp. n. on ph values is studied. native hds has a short-waved maximum position (325 nm) and a small half-width of the fluorescence spectrum (48nm). the change in the quantum yield of the enzyme fluorescence was parallel with the change of the enzymatic activity. triptophane residues of native hdc are located at hydrophobic region of the enzyme globula. the dependency of hdc flourescence parameters on ph value ... | 1975 | 1114 |
| the effect of silver ion binding and ph on the buoyant density of dna and its use in fractionating heterogeneous dna. | 1. the effect of ph on the buoyant density of the complexes of ag+ with dna has been studied using 3h-labeled human dna and several bacterial dnas to determine the conditions necessary for the maximum resolution of compositional heterogeneity. in neutral cs2so4 density gradients, ag+ complexes with (g - c)-rich components are always denser than those with (a - t)-rich components, since (g - c)rich dnas have a larger affinity for ag+ than (a - t)-rich dnas and their complexes are denser than (a - ... | 1976 | 4104 |
| membrane-reversible h+-atpase from micrococcus lysodeikticus. | treatment of phosphorylating fragments of bacterial membrane from micrococcus lysodeikticus with trypsin leads to increase atpase activity. as a result of this treatment, the membrane fragments acquire the ability to transform the atp energy into transmembrane difference in potential. dithiothreitol has a similar effect to that of trypsin on the membrane fragments from m. lysodeikticus. dicyclohexylcarbodimide inhibits atpase of the membrane fragments of m. lysodeikticus, and also the atpase-rea ... | 1976 | 4306 |
| bacteremia after tonsillectomy and adenectomy. | 40% of negative hemocultures become positive immediately after tonsillectomies and/or adenectomies. this bacteremia is asymptomatic and remains no longer than one hour. | 1976 | 4953 |
| catalytic implications of electrostatic potentials: the lytic activity of lysozymes as a model. | | 1976 | 5609 |
| the tom gibson memorial lecture. the microbiological role of nitrite in meat products. | | 1976 | 5778 |
| study of the structure of the histidine decarboxylase of micrococcus sp. n. by the method of circular dichroism. | ring dichroism spectra (rd) of histidine decarboxylase (hdc) from micrococcus sp. n. at the regions of peptide bonds (200-240 nm) and aromatic amino acids (250-300 nm) absorption are studied. the treatment of rd spectra according to methods of greenfield-fasman, saksena-vetlaufer and mayer permits to conclude that at the ph range within 4-8 the content of ordered structures of alpha-helix type comprises 20%, that of beta-structure type-40%, while the rest 40% are represented with polypeptide cha ... | 1976 | 6076 |
| exo-beta-n-acetylmuramidase--a novel hexosaminidase. production by bacillus subtilis b, purification and characterization. | exo-beta-n-acetylmuramidase, or beta-2-acetamido-3-o-(d-1-carboxyethyl)-2-deoxy-d-glucoside acetamidodeoxyglucohydrolase, is produced by bacillus subtilis b, growing in a succinate/peptone/salts medium, at the end of exponential growth and occurs partly in the medium and partly bound to the cells. a lysozyme digest of micrococcus lysodeikticus cell walls, o-2-acetamido-2-deoxy-beta-d-glucopyranosyl-(1 leads to 4)-2-acetamido-3-o-(d-1-carboxyethyl)-2-deoxy-d-glucose and o-2-acetamide-3-o-(d-1-car ... | 1976 | 6281 |
| isolation and partial characterization of the two major subunits of the bf1 factor (atpase) from micrococcus lysodeikticus and evidence for their glycoprotein nature. | | 1976 | 6334 |
| purification of several bacteriolytic enzymes by affinity chromatography on lysozyme-lysate of micrococcus lysodeikticus cell wall coupled with sepharose. | using lysozyme-lysate of micrococcus lysodeikticus cell wall coupled with sepharose, several bacteriolytic enzymes were purified from crude preparations of animal and microbial origin. quail egg-white, human milk and salivary lysozymes ec 3.2.1.17 were adsorbed onto the adsorbent at ph 5-7 and eluted with 2m nacl at ph 10. by means of these treatments, lysozymes were purified 20-250 fold with activity recoveries of 60-80%, and the quail lysozyme thus purified was shown to be discelectrophoretica ... | 1975 | 6436 |
| a transition state analog of lysozyme catalysis prepared from the bacterial cell wall tetrasaccharide. | treatment of the cell wall tetrasaccharide glcnacbeta(1 leads to 4)-murnac-beta(1 leads to 4)-glcnac-beta(1 leads to 4)-murnac with alkali resulted in the formation of the unsaturated tetrasaccharide glcnac-beta(1 leads to 4)-murnac-beta(1 leads to 4)-glcnac-beta(1 leads to 4)-delta2,3-2-acetamido-2-deoxy-d-glucoseen. the same compound was also formed by transglycosylation upon incubation of the unmodified tetrasaccharide with the unsaturated disaccharide glcnac-beta(1 leads to 4)-delta2,3-2-ace ... | 1976 | 6476 |
| microbial formation and degradation of dimethylamine. | dimethylamine was formed from trimethylamine in soils of different ph values. the rate of disappearance of the secondary amine from soil was affected by ph and was markedly reduced under anaerobiosis. the accumulation of dimethylamine in cultures of micrococcus sp. provided with trimethylamine depended on the nitrogen sources available to the bacterium but was not greatly influenced by the c-n ratio of the medium. dimethylamine and nitrite accumulated in large amounts at ph 6.0 to 8.0 in culture ... | 1976 | 7191 |
| non-heme iron proteins. the amino acid sequence of rubredoxin from desulfovibrio vulgaris. | a non-heme iron protein, rubredoxin has been isolated from the sulfate-reducing bacterium, desulfovibrio vulgaris, strain hildenborough. the complete amino acid sequence has been established. the 52 amino acid residues of the protein were aligned with the aid of tryptic and chymotryptic peptides and of a fragment produced by cleavage of the asn-gly bond (22-23) by hydroxylamine. the sequence of the first 30 residues of the molecule was determined using an automatic sequenator, after removal of t ... | 1976 | 7308 |
| simultaneous identification of t and b cells in blood smears using antibody coated bacteria. | | 1976 | 7360 |
| steric effects on penicillin-sensitive peptidoglycan synthesis in a membrane-wall system gaffkya homari. | residues 4 and 5 of the pentapeptide moiety, r-ala1-dglu2-lys3-dala4-dala5, of peptidoglycan play an important role in the donor phase of cross-linked glycan synthesis. to assess the role of these residues in this phase, a series of udp-murnac-peptides were biosynthesized with residues 4 and 5 replaced singly by either d-alpha-amino-n-butyric acid, d-norvaline, or d-valine. the six nucleotides were compared with udp-murnac-ala-dglu-lys-dala-dala (reference) in nascent (penicillin-insensitive) pe ... | 1976 | 8084 |
| studies on insect bacteriolytic enzymes--ii. some physical and enzymatic properties of lysozyme from haemolymph of galleria mellonella. | | 1976 | 9261 |
| distribution and effect of the weak acids 5,5-dimethyl-2,4-oxazolidinedione (dmo) and 2,4-dinitrophenol (dnp) in membrane vesicles of micrococcus denitrificans. | | 1976 | 9905 |
| microbiology of the female genital tract during pregnancy and parturition. | | 1976 | 10196 |
| serum bactericidal activity in the horseshoe crab, limulus polyphemus. | serum from the horseshoe crab, limulus polyphemus, was examined for bactericidal activity against five species of bacteria. greatest activity was found against pseudomonas putida and flavobacterium sp.; with the former, serum dilutions as high as 1:20 were capable of reducing viable counts by 50% within 2 h. bactericidal activity of a significantly lesser magnitude was demonstrated against serratia marcesencs and salmonella minnesota. no killing was seen when the lobster pathogen aerococcus viri ... | 1976 | 11188 |
| factors influencing lysozyme determinations by the lysoplate method. | some factors influencing the lysoplate method have been investigated. the influences of ph, ionic strength and temperature of the gel medium can be explained by considering the influence of these factors upon the diffusion rate rather than upon the enzymatic properties of lysozyme. salts and proteins present in a sample probably affect the results in a similar way. although the method has a variation coefficient around 10% and thus an acceptable precision, its accuracy is variable and doubtful. ... | 1977 | 12894 |
| biosynthesis of peptidoglycan in gaffkya homari. the incorporation of peptidoglycan into the cell wall and the direction of transpeptidation. | wall membrane enzyme preparations from gaffkya homari catalyze the formation of peptidoglycan from the precursor pairs: udp-n-acetylglucosamine + udp-n-acetylmuramyl-pentapeptide (udp-murnac-ala-dglu-lys-dala-dala) and also from udp-n-acetylglucosamine + udp-n-acetylmuramyl-tetrapeptide (udp-murnac-ala-dglu-lys-dala). part of the reaction products is soluble in 2% sodium dodecylsfulfate whereas the other part is bound to pre-existing cell wall peptidoglycan. the incorporation into cell wall take ... | 1976 | 12946 |
| use of clindamycin-2-phosphate in ophthalmology (author's transl). | clindamycin-2-phosphate was studied to evaluate its possible use in ophthalmology. 1. sensitivity of organisms isolated from human clinical materials: of 44 staphylococcal strains in vitro studied 13 were highly sensitive to 0.1 mug/ml and 28 were resistant to 100 mug/ml or more of clindamycin-2-phosphate. 2. in experiments with rabbits, clindamycin-2-phosphate showed superior penetration into the ocular tissues after intravenous injection than after intramuscular injection. 3. clindamycin-2-pho ... | 1977 | 14269 |
| acute sepsis caused by "gaffkya tetragena" in adult with hypogammaglobulinemia (author's transl). | a case of acute sepsis caused by gaffkya tetragena in an adult with acquired hypogammaglobulinemia has been described. the authors pointout the importance that particular conditions of disreactivity and/or of immunodeficiency can play in the acquistion of pathogenicity by gaffkya tetragena. in the case under discussion a high deficit of igg and iga was demonstrable, which had previously caused a long series of infective bacterial diseases. | 1976 | 14599 |
| separation of dna-dependent dna polymerase activities in micrococcus radiodurans. | dna polymerase activities in micrococcus radiodurans were separated into two fractions after purification more than 2000 fold. they differ in ph optimum and residual activities in the absence of a full deoxyribonucleoside triphosphates complement. nad partly inhibited one of the activities. both activities were eluted as a single peak on gel filtration and sedimented at the same rate on glycerol gradient centrifugation. molecular weight 140000 was calculated from stokes radius and sedimentation ... | 1977 | 14686 |
| occurrence of gaffkaemia in lobsters in norway. | | 1977 | 15430 |
| importance of glycerol and fatty acid residues on the ionic properties of phosphatidylglycerols at the air-water interface. | ionic properties of didodecanoylphosphatidylglycerol (c12pg), didodecanolyphosphatidyl-l'-propanol (c12pp), di-(12-methyl, 13-methyl)-pentadecanoylphosphatidylglycerols (c15pg) and dihexadecanoylphosphatidylglycerol (c16pg) have been studied at the air-water interface using titration experiments at constant ionic strength and film expansion experiments at constant ph, with li+, na+, k+ and cs+ in the subphase. for each lipid, the apparent pk in the surface is strongly dependent on the subphase s ... | 1977 | 15730 |
| the testing of the antibiotic sensitivity of bacteria on an agar medium: the problem of a double zone of inhibition. | when the sensitivity of micrococcus luteus attc9341 to streptomycin, erythromycin, oleandomycin and spiramycin was tested by an agar diffusion method using antibiotic impregnated filter paper disks on unbuffered penassay seed agar two zones of inhibition were observed around the disks after an incubation period of 24 hours at 30 degrees c. the ph of the m. luteus seeded penassay seed agar was measured before and after 24 hours incubation at 30 degrees c and found to be 6.6 and 8.7, respectively. ... | 1977 | 16428 |
| microflora in the healthy gingival sulcus in man. | the roll tube culture technique was utilized to examine quantitatively and qualitatively the predominant cultivable microflora inhabiting the clinically healthy gingival sulcus. seven periodontists aged 32-54 years were included in the study. from a total of 350 isolates, 85 (24.3%) were obligate anaerobes, 53 (15.0%) were gram negative, and 197 (56.3%) were categorized as rods. actinomycetes predominated in three samples. four samples were dominated by streptococcus species. the flora as reveal ... | 1977 | 17152 |
| in vitro inhibition of growth of neisseria gonorrhoeae by genital microorganisms. | the ability of microorganisms present in titers of over 10(5)/ml in the vaginal or cervical secretions to inhibit growth of n. gonorrhoeae in vitro was tested. study of a strain of n. gonorrhoeae against 77 microorganisms demonstrated that most strans of s. epidermidis, s. aureus and the one "gaffkya anaerobia" interfered with the growth of the n. gonorrhoeae. a minority of strains of s. viridans, neisseria, candida and bifidobacterium demonstrated interference. no strains of enterococcus, dipth ... | 1977 | 17163 |
| the bacteriology of skin cysts. | thirty-nine clinically uninflamed cysts of the three most common varieties, epidermoid cysts, trichilemmal cysts and steatocystoma multiplex were removed under sterile conditions and the contents cultured under aerobic and anaerobic conditions. seventy-three percent of epidermoid cysts grew significant numbers of organisms whereas none of the trichilemmal cysts did so. the organisms found were the common skin commensals, staphylococcus epidermidis biotype i, anaerobic gram positive cocci of the ... | 1977 | 17428 |
| study of the nature of the associated microflora in the prolonged substrateless cultivation of beet plants. | the purpose of the present investigation was to study variations in the population density of microorganisms, their qualitative and quantitative composition during substrate-free cultivation of beats. the results obtained show that the microbial number in the nutrient solution increased by the time of intensive growth of beats and decreased by the time of harvesting. the number of root microorganisms was much greater than the total number of microorganisms in the nutrient solution. oligonitrophi ... | 1977 | 17774 |
| quantitative study and typization of aerobic bacteria in the conjunctival sacs of healthy eyes. | we carried out a qualitative and quantitative bacteriological research into the conjunctives of 220 subjects in good health mostly women (180) exempt from recent or previous eyes diseases. in order to gather conjunctival bacteria we employed with a few variations hadley's et al. (1973 12) method. among the calculated bacterial flora we found as the most numerous ones staphylococcus epidermidis (mean valor 1960/ml), then streptococci (480/ml), micrococci (465/ml), diphteroides (360/ml), enterococ ... | 1976 | 18118 |
| analysis of the activity of micrococcus luteus endonucleases with respect to gamma-irradiated dna. | endonucleases from micrococcus luteus that induce single-strand breaks in gamma-irradiated dna have been separated chromatographycally into two groups. the first group involves two different enzymes: ap-endonuclease ii (mol. weight 30 000) and ap, uv-endonuclease i (mol. weight 15 000) that recognize alkali-labile lesions in gamma-irradiated dna and apurinic sites in dna heated at 70 degrees c, ph 6.08 ap-endonuclease ii in cooperation with dna polymerase from m. luteus and t4 phage-induced poly ... | 1977 | 20161 |
| purification and characterization of lysozyme produced by streptomyces erythraeus. | a species of lysozyme (se lysozyme) was purified from culture filtrate of streptomyces erythraeus. the enzyme has a molecular weight of 18,500 as determined by ultracentrifugation. its isoelectric point is 9.5, and it shows optimal activity at ph 4.0 with an optimal ionic strength of 0.1. investigation of the substrate specificity showed se lysozyme to be an n-acetyl-muramidase. the simplest product in the digest of cell walls of micrococcus lysodeikticus was identified as a disaccharide, glcnac ... | 1978 | 25274 |
| the activities in vitro of dd-carboxypeptidase and ld-carboxypeptidase of gaffkya homari during biosynthesis of peptidoglycan. | | 1978 | 25767 |
| biochemical aspects of cardiac muscle differentiation. | experiments were designed to determine whether dna synthesis ceases in terminally differentiating cardiac muscle of the rat because the activity of the putative replicative dna polymerase (dna polymerase alpha) is lost or whether the activity of this enzyme is lost because dna synthesis ceases. dna-template availability and 3'-hydroxyl termini in nuclei and chromatin, isolated from cardiac muscle at various times during the developmental period in which dna synthesis and the activity of dna poly ... | 1978 | 26332 |
| cell wall autolysis in log phase cells of micrococcus lysodeikticus (luteus). | log phase cells of micrococcus lysodeikticus (luteus) ifo 3333 autolyzed when incubated at 37 c in 0.01 m sodium-phosphate buffer ph 7.5. the enzyme involved in the autolysis was recovered mainly in an aqueous phase from cytoplasmic membranes and cytoplasmic materials treated with n-butanol, and proved to be an n-acetylmuramyl-l-alanine amidase. the autolysis of log phase cells suspended in autolyzing buffer was depressed by the addition of trypsin to the buffer. | 1978 | 27703 |
| effect of prolonged occlusion on the microbial flora, ph, carbon dioxide and transepidermal water loss on human skin. | the effects of prolonged occlusion on the normal microbial skin flora, ph, transepidermal water loss (tewl) and carbon dioxide emission rate (cder) were studied. the total average counts before occlusion were 1.8 x 10(2)/cm(2) and increased to 4.5 x 10(6) on day 5. the highest counts were noted on day 4(9.8 x 10(7)/cm(2)). the composition changed: controls comprised of 63% coagulase negative staphylococci, 6% micrococci, 17% diphtheroids and 6% bacilli. after 5 days of occlusion, the percent com ... | 1978 | 31403 |
| silica gel media for isolating and studying bacteria under hydrostatic pressure. | individual colonies of micrococcus euryhalis and of a marine bacterial isolate were grown in pour tubes under hydrostatic pressure. the medium was prepared in a silica sol, and gelation was effected at 4 degrees c by addition of salts to achieve concentrations found in seawater. | 1978 | 32836 |
| chemical modification of the surfaces of bacterial cell walls. | the surfaces of the isolated cell walls of four bacterial species were studied by microelectrophoresis following chemical treatments intended to remove specific charged groups. acid-base titrations of the walls were used to assess specificity and extent of the modifications. carboxyl groups were specifically and completely modified by activation with a water-soluble carbodiimide and subsequent reaction with a nucleophile, such as glycinamide, to give an uncharged ph-stable product. aqueous media ... | 1978 | 33400 |
| equilibrium constants under physiological conditions for the reactions of polynucleotide phosphorylase and rna polymerase. | | 1979 | 33992 |
| purification and properties of rabbit alveolar macrophage lysozyme. | lysozyme was isolated from bacillus calmette-guerin-elicited rabbit alveolar macrophages by acid extraction and purified to homogeneity by a single-column procedure. yields of the purified enzyme averaged between 20 and 30 mg per rabbit, values far in excess of those obtained with previously published methods. rabbit lysozyme has a molecular weight of 14,300 and exhibits optimal lytic activity against micrococcus lysodeikticus at an ionic strength of 0.04, ph 6.5. our results indicate that lysoz ... | 1979 | 37167 |
| biochemical properties of penicillin amidohydrolase from micrococcus luteus. | some biochemical properties of whole-cell penicillin amidohydrolase from micrococcus luteus have been studied. this whole-cell enzyme showed its maximal activity at 36 degrees c at ph 7.5. it was found that the activation energy of this enzyme was 8.03 kcal (ca. 33.6 kj) per mol, and this amidohydrolase showed first-order decay at 36 degrees c. the penicillin amidohydrolase was deactivated rapidly at temperatures above 50 degrees c during storage or preincubation for 24 h. the michaelis constant ... | 1979 | 39505 |
| the amino acid sequence of a variable region of rabbit b4 chain from an anti-siii antibody: comparison with light chains of the same sub-group from anti-a-variant carbohydrate antibodies. | | 1979 | 42017 |
| a medium for commercial production of the halophilic micrococcus nuclease. | a simple synthetic medium (glutamate-sucrose medium) was devised for production, during growth in shaken flasks, of extracellular halophilic nuclease (nuclease h) by a moderate halophile, micrococcus varians subsp. halophilus. a simple medium consisting of 0.7% ammonium sulfate, 1.0% glucose, minerals, three vitamins, and 2 m nacl gave good growth and excellent production of nuclease h in a jar fermentor when the ph was adjusted to 7.5 to 8.0 during cultivation. | 1979 | 44225 |
| characterization of the autolytic enzymes of clostridium perfringens. | clostridium perfringens and isolated walls of this organism autolysed rapidly when incubated in buffer at ph 7.0 with the release of free-reducing groups but no n-terminal amino acids. the predominant autolytic enzyme was an endo-beta-n-acetylglucosaminidase, and an endo-beta-n-acetylmuramidase was also present. the autolytic enzymes could be solubilized by extraction of the organisms with 5 m-licl and would then subsequently bind to and rapidly lyse walls of micrococcus luteus and, more slowly, ... | 1979 | 44314 |
| [animal experiments with combined local immunization against an influenza virus disease (author's transl)]. | | 1979 | 44774 |
| cytopathology of nipple discharge due to pityrosporum orbiculare and cocci in an elderly woman. | a case has been described where cytologic examination first showed a fungus infection as the possible cause of a nipple discharge of long duration. the discharge was found to be associated with pityrosporum orbiculare, micrococcus tetragenes and staphylococci (coagulase negative). administration of an antibiotic that inhibited the bacteria cured the condition. | 1975 | 46660 |
| proceedings: importance of dna size for integration in plant materials. | | 1975 | 50809 |
| proceedings: integration and replication of bacterial dna in barley root cells. | | 1975 | 50810 |
| proceedings: comparative effects of n-bromosuccinimide on lysozymes of hen egg-white and of human origin. | | 1975 | 50811 |
| micrococcal urinary-tract infections in young women. | in a prospective study in young women, novobiocin-resistant subgroup-3 micrococci were the second commonest cause, after escherichia coli; of acute urinary infections. proteus mirabilis was the only other causative organism. symptoms, pyuria, or possible aetiological factors were the same in micrococcal and coliform infections. the infecting micrococcus "biotype" was only rarely found among the normal flora of the genitourinary tract of young women, though other micrococci and staphylococci were ... | 1975 | 51404 |
| isolation and characterization of homogenous rabbit antibodies to micrococcus lysodeikticus with specificity to the peptidoglycan and to the glucose-n-acetylaminomannuronic acid polymer. | the antibody response of rabbits to micrococcus lysodeikticus is characterized by the production of a high concentration of antibodies which manifest markedly reduced heterogenicity. the specificity of these antibodies was studied and it revealed that m. lysodeikticus contains 2 major antigens: both the glucose-n-acetyl-aminomannuronic acid polymer obtained by formamide extraction of the cell walls and peptidoglycan solubilized by ultrasonic treatment gave precipitin reactions with hyperimmune a ... | 1975 | 52238 |
| some characteristics of the dna-tyrocidine complex and a possible mechanism of the gramicidin action. | 1. the cyclic peptide antibiotic tyrocidine which inhibits rna synthesis in vitro by forming a complex with the dna (schazschneider, b., ristow, h. and kleinkauf, h. (1974) nature 249, 757-759) induces hypochromicity of the dna. the complex dissociates at elevated temperatures but which are below the melting temperature of the dna. 2. the linear peptide antibiotic gramicidin which reverses the inhibitory effect of tyrocidine (ristow, h., schazschneider, b., bauer, k. and kleinkauf, h. (1975) bio ... | 1975 | 53075 |
| proceedings: some aspects of the production of a restricted immune response. | | 1975 | 54121 |
| [change in lipid-protein interactions in the membranes of bacteria exposed to gramicidin s]. | effect of cyclopeptide antibiotic gramicidin s on some enzymes and physical state of isolated micrococcus lysodeikticus membranes is studied. malate and lactate dehydrogenases were monotonously inhibited under the increase of gramicidin s concentration, while the activity of nadh-dehydrogenase firstly decreased and then reversed to the initial level under further increase of gramicidin s concentration. the oxygen uptake under oxidation of nadh and malate with membranes almost completely inhibite ... | 1976 | 58672 |
| dna polymerases of eukaryotes. | | 1975 | 58795 |
| immunochemical study of the peptidoglycan of gram-negative bacteria. | the specificity of antibodies directed against the peptidoglycan of gram-negative bacteria was studied. the peptidoglycans of proteus vulgaris, escherichia coli, moraxella glucidolytica, neisseria perflava, give identical precipitin reactions. by means of inhibition studies with various peptidoglycan subunits and synthetic peptides, it was shown that the antibodies are essentially directed against the peptide moiety of the peptidoglycan: l-ala-d-glu (l)-mesoa2pm-(l)-d-ala, that the peptide react ... | 1976 | 60233 |
| the immune response to micrococcus lysodeikticus. 1. evolution of the response in two litter mates. | | 1976 | 64217 |
| the immune response to micrococcls lysodeikticus. 2. physico-chemical studies of two antibodies. | | 1976 | 64218 |
| characterization of homogeneous antibodies in hares [proceedings]. | | 1976 | 66033 |
| probing lymphoid systems with anti-micrococcus antibodies. i. effect on lymphocyte response to mitogens in vitro [proceedings]. | | 1977 | 68725 |
| synthesis and properties of 5-thiomercuri derivatives of pyrimidine nucleotides [proceedings]. | | 1977 | 68750 |
| use of antimicrococcus immunity to produce high titre antibodies of restricted heterogeneity [proceedings]. | | 1977 | 68754 |
| interactions of the lectins pha, cona and antimicrosoccus with blood cells of different species and erhlich carcinoma cells. | | 1976 | 71077 |
| restriction of the anti-bovine serum albumin response in rabbits immunized with micrococcus lysodeikticus. | rabbits capable of producing antibodies of restricted heterogeneity in response to micrococcus lysodeikticus are equally capable of producing antibodies of restricted heterogeneity to bovine serum albumin. these antibodies are produced when animals are simultaneously injected with micrococcus and bsa and their specificity is restricted to a small number of epitopes. these results suggest that micrococcal vaccines can induce the restriction of heterogeneity in antibodies raised against totally un ... | 1977 | 71263 |
| "micrococci" and urinary infection. | | 1977 | 71564 |
| comparative immunological study of catalases in the genus micrococcus. | double immunodiffusion tests were performed with crude extracts from various micrococcus species and antisera against catalase of micrococcus luteus ccm 169. cell-free extracts of m. lylae atcc 27566 exhibited good cross-reaction. cell-free extracts or catalase enriched preparations of m. varians reacted very weakly and no reaction has been found with preparation of m. kristinae, m. nishinomiyaensis, m. roseus and m. sedentarius. the quantitative microcomplement fixation assay also revealed a cl ... | 1977 | 71880 |
| micrococci and urinary infection. | | 1977 | 73917 |
| immunological properties of membrane-bound adenosine triphosphatase: immunological identification of rutamycin-sensitive f0.f1atpase from micrococcus luteus atcc 4698 established by crossed immunoelectrophoresis. | (1) f0.f1atpase (ec 3.6.1.3) from micrococcus luteus atcc 4698 was solubilized from plasma membranes by the non-ionic detergent triton x-100 in the presence of 0.05 m mgcl2. (2) the antibiotics rutamycin, dio-9, quercetin, oligomycin, botrycidin, efrapeptin, leucinostatin, valinomycin, and venturicidin as well as n,n'-dicyclohexylcarbodiimide and dinitrophenol are potent inhibitors of f0.f1atpase activity.(3) f0.f1atpase activity is completely inhibited by anti-f1atpase antibodies. the inhibitio ... | 1978 | 77681 |
| on the fate of exogenously supplied bacterial dna in soybean [proceedings]. | | 1977 | 79341 |
| fate of bacterial dna in melon plants [proceedings]. | | 1977 | 79353 |
| non-specific tumour immunotherapy by micrococcus lysodeikticus and related polysaccharides [proceedings]. | | 1977 | 79358 |
| apparent destruction and reutilization of heterologous dna applied to seeds of lactuca sativa [proceedings]. | | 1977 | 79399 |
| uptake and retention of heterologous dna applied to seeds of matthiola incana [proceedings]. | | 1977 | 79400 |
| comparison of micrococcus, bcg and related polysaccharides in the specific and non-specific immune resistance to murine l1210 leukemia [proceedings]. | | 1978 | 81034 |
| [starch agar for detection of pigment formation in the representatives of the micrococcaceae family]. | | 1978 | 82658 |
| molecular mechanisms involved in the production of chromosomal aberrations. i. utilization of neurospora endonuclease for the study of aberration production in g2 stage of the cell cycle. | chinese hamster ovary cells (cho) were x-irradiated in g2 stage of the cell cycle and immediately treated, in the presence of inactivated sendai virus, with neurospora endonuclease (e.c. 3.1.4.), an enzyme which is specific for cleaving single-stranded dna. with this treatment, the frequencies of all types of chromosome aberrations increased when compared to x-irradiated controls. these results are interpreted as due to the conversion of some of the x-ray induced single-stranded dna breaks into ... | 1978 | 83535 |
| comparison between the effects of micrococcus, bcg and related polysaccharides in preclinical chemo-immunotherapy of murine l 1210 leukaemia [proceedings]. | | 1978 | 84653 |
| [study of the reaction between gramicidin s and bacterial membranes by the proton magnetic resonance technique]. | | 1979 | 86412 |
| [compartmentation of gramicidin 5 in membranes of sensitive bacteria and protein-lipid interactions]. | gramicidin s is sorbed on the isolated membranes of granicidin-sensitive micrococcus lysodeikticus strain. the antibiotic inhibits the membrane malate dehydrogenase within the temperature range of 9--42 degrees c, i.e. under conditions of gel and liquid-crystalline lipid state; however its effect at 10 degrees c is 10 times as low as is observed at 42 degrees c. the inhibitory effect of gramicidin s on malate dehydrogenase can be eliminated and the antibiotic can be removed from the membrane by ... | 1979 | 88965 |
| [heterogeneity in organization of lipids in the bacterial membrane]. | the lipids of micrococcus lysodeikticus membranes were 50%-substituted by phosphatidyl choline using lipid-exchanging proteins isolated from rat liver. the incorporation of phosphatidyl choline into the membrane did not significantly change the malate dehydrogenase activity and the temperature dependence activity in the arrhenius plots for the enzyme. gramicidin s--modifier of membrane lipids--had similar effects both on the intact membranes and on the phosphatidyl-enriched membranes. a conclusi ... | 1979 | 88966 |
| [lysis of micrococcus lysodeikticus protoplasts by gramicidin s derivatives]. | gramicidin s derivatives with the substituted amino groups of ornithines (carbamoylgramicidin and diacetylgramicidin) possess nearly the same level of the lytic activity when acting on micrococcus lysodeikticus protoplasts in a 1 m sucrose solution as the original antibiotic. the lytic activity of gramicidin s and these derivatives considerably increases in a solution of sucrose in phosphate buffer. the dependence of the lytic activity of gramicidin on its concentration is of a complex character ... | 1979 | 90326 |
| effect of micrococcus, bcg and structurally related polysaccharides on the adjuvanticity to a t cell-dependent antigen [proceedings]. | | 1979 | 93950 |
| a new quantitative fluorimetric assay for phagocytosis of bacteria [proceedings]. | | 1979 | 93952 |
| [study of membrane proteins from microccus lysodeikticus using immunochemical methods]. | using immunoelectrophoresis, the antigenicity of various protein fractions of the micrococcus lysodeikticus membranes was evaluated. it was shown that both the peripheral and integral membrane proteins possess the antigenic determinants. the antiserum exhausted by the m. lysodeikticus mebranes loses its ability to interact with intergral proteins, which are not solubilized by triton x-100. it was thus assumed that the integral proteins are exposed on the membrane surface constantly or periodical ... | 1979 | 94839 |
| mode of action of iturin a, an antibiotic isolated from bacillus subtilis, on micrococcus luteus. | | 1978 | 96818 |
| an improved electrophoretic method for identifying antibiotics with special reference to animal tissues and animal feeding stuffs. | | 1978 | 97257 |
| a separation of staphylococci and micrococci based on serological reactivity with antiserum specific for polyglycerophosphate. | a serological reaction with the antiserum against heterophile polyglycerophosphate (pgp) was evaluated for genus level differentiation among strains of staphylococcus and micrococcus-sarcina spp.. hot saline extracts from whole cells of staphylococcus spp. strongly reacted with the pgp antiserum, whereas those of micrococcus-sarcina spp. did not. likewise, phenol-water extracts from whole cells of micrococcus-sarcina spp. were not reactive with the pgp antiserum, although the extracts of staphyl ... | 1978 | 97498 |
| microbial degradation of [c14c]polystyrene and 1,3-diphenylbutane. | microbial degradation of [beta-14c]polystyrene and 1,3-diphenylbutane, a compound structurally representing the smallest repeating unit of styrene (dimer), was investigated in soil and liquid enrichment cultures. degradation rates in soil, as determined by 14co2 evolution from applied [14c]polystyrene, varied from 1.5 to 3.0% for a 4-month period. although relatively low, these percentages were 15 to 30 times greater than values previously reported. enrichment cultures, containing 1,3-diphenylbu ... | 1978 | 98222 |
| new peroxides and their antimicrobial activity. | antimicrobial activity of hydroperoxides and cyclic peroxides has been studied. 2,4-dihydroperoxy-2,4-dimethylpentane (1) and 2,5-dihydroperoxy-2,5-dimethylhexane (2) were active against microorganism, but 1 showed a large specially against c. albicans, m. luteus and b. subtilis with a mic value of 31.5 mcg/ml. | 1978 | 98815 |
| formation of spirodilactone of 4-(2'-carboxyphenyl)-4,4-dihydroxybutyrate from 2-succinylbenzoate in cell-free extracts of micrococcus luteus. | the enzyme mediated atp- and, to a lesser extent, coash-dependent synthesis of the spirodilactone of 4-(2'-carboxyphenyl)-4,4-dihydroxybutyrate from 2-succinylbenzoate has been demonstrated in membrane-free extracts of micrococcus luteus and escherichia coli. the suggestion is made that the spirodilactone is the product of an aberrant reaction involving a compound that is normally an intermediate in the conversion of 2-succinylbenzoate to 1,4-dihydroxy-2-naphthoate. | 1978 | 99177 |
| addition of poly(adenylic acid) to rna using polynucleotide phosphorylase: an improved method for electron microscopic visualization of rna-dna hybrids. | we have observed that the enzyme polynucleotide phosphorylas from m. luteus or from e. coli will polymerize adenosine (a) from adenosine diphosphate onto 3' ends of rna molecules. for gene mapping, the poly(a)-tailed rna is hybridized to its complementary sequence on a longer dna strand. the position of the poly(a)tail, and thus the position of the 3' end of the rna on the dna strand, can then be observed by electron microscopy. our preferred mapping technique involves the synthesis of a poly(a) ... | 1978 | 100138 |
| [membranes of bacteria and mechanism of action of the antibiotic gramicidin s]. | the cyclopeptide antibiotic gramicidin s taken at a concentration of 100--200 mkg/mg membrane protein rapidly increases the permeability of m. lysodeikticus protoplast membranes for substrates of respiratory chain and exogenous cytochromes c. prolonged incubation of gramicidin s with protoplasts results in their lysis which is more fast at low temperatures. in contrast to natural gramicidin, a derivative of gramicidin s with acetylated amino groups does not inhibit either the micrococcus membran ... | 1977 | 66939 |
| mode of inhibition of herpes simplex virus dna polymerase by phosphonoacetate. | phosphonoacetate is a highly specific inhibitor of herpes simplex virus-induced dna polymerase. sensitivity of herpesvirus type 1 or type 2 induced dna polymerase to the drug was similar. however, dna polymerases from other sources such as the host cells (wi-38), micrococcus luteus, and hepatitis b virus were highly resistant. in addition, escherichia coli rna polymerase and reverse transcriptase of rous sarcoma virus were also insensitive to the drug. enzyme kinetic studies showed that inhibiti ... | 1975 | 53071 |
| microbial nitrosamine formation in palm wine: in vitro n-nitrosation by cell suspensions. | the ability of certain species of bacteria and yeasts that usually contaminate fresh palm sap to induce the formation of the carcinogen dimethylnitrosamine from suitable precursors (trimethylamine, dimethylamine, nitrate, and nitrite) was investigated in vitro under neutral and acid ph conditions. in the incubation media containing cell suspensions of each species of test organism, namely, aerobacter, micrococcus, serratia, saccharomyces, and two unidentified yeasts and the precursors, added in ... | 1979 | 36441 |
| the effect of ionophores on phosphate and arsenate transport in micrococcus lysodeikticus. | | 1977 | 21813 |