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quantitative study and typization of aerobic bacteria in the conjunctival sacs of healthy eyes.we carried out a qualitative and quantitative bacteriological research into the conjunctives of 220 subjects in good health mostly women (180) exempt from recent or previous eyes diseases. in order to gather conjunctival bacteria we employed with a few variations hadley's et al. (1973 12) method. among the calculated bacterial flora we found as the most numerous ones staphylococcus epidermidis (mean valor 1960/ml), then streptococci (480/ml), micrococci (465/ml), diphteroides (360/ml), enterococ ...197618118
lipids of the streptomycettes. structural investigation and biological interrelation a review.during a systematic investigation of lipids of streptomycetes a series of compounds of biochemical and microbiological interest have been isolated and characterized. these include several menaquinones, glycosyl diglycerides (glucuronosyl and isoladobinosym diglycerides), two ornithino lipids and a diol phospholipid. some of these lipids were not known previously as constituents of streptomycete cells although they have been encountered elsewhere; others have proved to be novel lipids. the result ...197896946
effect of agar percentage, agar thickness, and medium constituents on antibiotics assay by disc diffusion method.the factors affecting the sensitivity of nafcillin, vancomycin, oleandomycin, chloramphenicol, methacycline and novobiocin assay, using the paper disc diffusion method, were studied. agar concentration and pour volume were found to influence the assay sensitivity of antibiotics. the type of assay medium was effective e.g. brain heart infusion agar was the best medium for nafcillin and methacycline; synthetic amino acid medium for oleandomycin; muller hinton agar for vancomycin and chloramphenico ...197897678
[examination of market carps for residues of antimicrobial agents].the residues of inhibitory substances in the muscle, hepatopancreas, bile, and pyloric caeca of 30 carps from ponds south of brno were examined by the microbiological diffusion method. the following testing micro-organisms were used: b. cereus var. mycoides (atcc 11778), b. subtilis (atcc 6633), sarcina lutea (atcc 9341), and micrococcus flavus (atcc 10240). residues of inhibitory substances were not found in any of the muscle samples tested. the rest of the tested tissues showed, in some cases, ...1978100930
anti-riboflavin activity of 8n-alkyl analogues of roseoflavin in some gram-positive bacteria.three new 8n-alkyl analogues of roseoflavin (mm), i.e., 8-ethylamino- (eh), 8-methylethylamino- (me), 8-diethylamino-8-demethyl-d-riboflavin (ee), their tetraacetates, and 8-amino-8-demethyl-d-riboflavin (hh) tetraacetate, were synthesized. a relation between the anti-riboflavin activity and the chemical structure of 8n-alkyl analogues (8n-methyl, ethyl) was studied by a restoration by riboflavin (rf) of inhibitory effect of the analogues on a growth of gram-positive bacteria, i.e., sarcina lute ...1978101644
cc-1065 (nsc-298223), a new antitumor antibiotic. production, in vitro biological activity, microbiological assays and taxonomy of the producing microorganism.a new antitumor antibiotic is produced in fermentation liquors of streptomyces zelensis sp.n. the antibiotic is biologically active at extremely low concentrations. at 40 pg/ml, it inhibited 90% of the growth of l1210 cells in culture in tube dilution assays. the minimal inhibitory concentrations against gram-positive bacteria is between 1 approximately 10 ng/ml, while these values for gram-negative bacteria and fungi are mostly under 1 microgram/ml. a microbiological assay with bacillus subtili ...1978104946
[study of the effectiveness of new quaternary ammonium salts against selected strains of bacteria and fungi. sensitivity of microbial strains isolated from patients to various ammonium salts]. 1979117676
survey of antibiotic residues in canadian slaughter animals.kidneys and urine of cattle, swine, sheep and chickens were tested for bacterial growth inhibitors using bacillus subtilis and sarcina lutea as test organisms. results were as follows: 211 beef kidneys four positive, 611 swine kidneys five positive, 27 sheep and 120 chicken kidneys all negative, 2108 beef urine 76 positive, 2409 swine urine 186 positive, 176 sheep urine 17 positive. strongest reactions were obtained with b. subtilis on phosphate buffered ph 6.0 plates. larger zones were produced ...1975236082
excystment of axenically prepared cysts of hartmanella culbertsoni.axenically prepared cysts of hartmannella culbertsoni readily excysted in the presence of heat stable factors prepared from escherichia coli, klebsiella aerogenes, staphylococcus aureus, sarcina lutea, bacillus subtilis, bacillus megaterium and several fungi. peptone, proteose peptone, tryptone or amino acids also promoted excystment. crowding of the cysts and dilution of bacterial extracts adversely affected the excystment. continual presence of the factors in the medium was essential for excys ...1977319194
bactericidal activity of a superoxide anion-generating system. a model for the polymorphonuclear leukocyte.the acetaldehyde-xanthine oxidase system in the presence and absence of myeloperoxidase (mpo) and chloride has been employed as a model of the oxygen-dependent antimicrobial systems of the pmn. the unsupplemented xanthine oxidase system was bactericidal at relatively high acetaldehyde concentrations. the bactericidal activity was inhibited by superoxide dismutase (sod), catalase, the hydroxyl radical (oh.) scavengers, mannitol and benzoate, the singlet oxygen (1o2) quenchers, azide, histidine, a ...1979216766
pharmacokinetics of pivmecillinam.1 the plasma concentration/time curves of ampicillin and mecillinam in normal subjects were measured after oral administration of ampicillin (500 mg) and pivmecillinam (400 and 600 mg). 2 similar plasma concentration/time curves of ampicillin and mecillinam in the starved normal subjects followed oral administration of ampicillin (500 mg) and pivmecillinam (600 mg). 3 the plasma concentration/time curve of mecillinam was measured in the same normal subjects after oral administration of pivmecill ...1977197981
synthesis and some biological properties of 6-aminopenicillanic derivatives.the properties of 33 6-aminopenicillanic derivates were described, the majority of which and particularly all 3,4-disubstituted phenyl derivatives, are new compounds. these derivatives were synthesized by three different methods consisting in treating of 6-aminopenicillanic acid (6-apa) with appropriate phenylthio -- and phenoxyalkanecarboxylic acids. the method consisting in use of aryloxyketenes is new and claimed by the patent applications. all synthesized compounds were screened for their bi ...1977193092
classification of micrococci on the basis of deoxyribonucleic acid homology.the dna homology relationships of 25 micrococci (15 strains of micrococcus, eight strains of sarcina and two strains of staphylococcus) were studied by the deoxyribonucleic acid hybridization method using nuclease s1, an endonuclease specific for single-stranded dna molecules. nineteen of the strains were classified into three groups. group i contained micrococcus lysodeikticus iami056, m. luteus iami1010, m. flavus iami2005 and iami2006, sarcina flava iami2007 and iami1006. s. subflava iami2009 ...1976180238
enzymatic determination of butane-2,3-diol in wines. 1976177810
a facile method of purification of neocarzinostatin, an antitumor protein.a three-step column chromatographic method for the purification of neocarzinostatin (ncs) from a crude preparation was described. the purified material was homogeneous by acrylamide gel electrophoresis, isoelectric focusing, amino terminal analysis, and immunologic criteria. purified ncs was 40 times as active in the inhibition of growth of sarcina lutea and twice as active against ccrf-cem human leukemia cells in vitro as was the starting material. when assayed against p388 and l1210 mouse leuk ...1977140942
enumeration and identification of human leukemic lymphocytes by their natural binding of bacteria.the recently described property of bacteria to bind to human lymphocytes was used to distinguish between normal and chronic leukemic lymphocyte (cll) populations. strains of the following bacteria were used in this study: arizona hinshawii, escherichia coli strains 1 and 2, bacillus globigii, brucella melitensis, corynebacterium diphtheriae strains 1 and 2, corynebacterium xerosis, sarcina lutea, staphylococcus aureus, and staphylococcus epidermidis. for identification of immunoglobulin-bearing ...1977404035
mechanism of dna degradation induced by neocarzinostatin in bacillus subtilis.when logarithmically growing bacillus subtilis cells were exposed to the antitumor protein, neocarzinostatin (ncs), at a concentration of 50 mug/ml, cellular dna was gradually degraded into an acid-soluble form (up to 60 percent of total dna). the degradation appeared to initiate at the growing regions of dna and to proceed sequentially from the nascent regions to preexistent dna. concomitantly with, or perhaps as a consequence of, the degradation of growing regions, dna detached from the cell m ...1975123897
[distribution of erythromycin following intramuscular administration in laboratory animals].levels of erythromycin in the blood and organs of rabbits, rats and mice were determined after the i. m. implantation of the drug erythromycin inj. ad usum vet. at doses of 10 mg kg-1 live weight (rabbits), and/or 25 mg kg-1 (rats) and 1 mg pro toto--approximately 40 mg kg-1 (mice). a diffusion plate method and the germ sarcina lutea ccm 552 were used to find out the antibiotic in samples. sensitivity of the method used was 0.025 microgram ml-1 (g). in rabbits the level of the antibiotic was mai ...1978102070
[methods of demonstrating antibiotics residues in meat, organs and eggs]. 1977416557
tissue distribution and residues of oxytetracycline in normal and emergency-slaughtered ruminants.the concentration of oxytetracycline (oct) in the blood, kidneys urine and meat was determined in a group of clinically normal dairy cows slaughtered at different times after treatment. drug concentrations were similarly determined in a group of emergency-slaughtered dairy cows and calves. distribution pattern of the drug in the body, expressed as muscle-drip-to-serum and kidney-cortex-to-serum ratios of the drug concentrations were determined in clinically normal cows and these ratios were comp ...1978417423
erythromycin excretion in sheep and its relation to antibacterial effect. 1978417532
secondary fermentation in the runen of a sheep given a diet based on molasses.1. the extent of conversion of acetate-carbon to carbon dioxide in the rumen of a 40 kg wether consuming 1 kg molasses/d was estimated using isotope-tracer-dilution techniques. 2. there was a high rate of conversion of acetate to co2 (6.0 g c/d) in the rumen. there were high concentrations in the rumen of methanosarcina approximately 6 x 10(9)/ml which represents a significant proportion of the rumen bacterial biomass. these organisms are usually found in mud and sludge and are capable of oxidiz ...1979427091
a separation of staphylococci and micrococci based on serological reactivity with antiserum specific for polyglycerophosphate.a serological reaction with the antiserum against heterophile polyglycerophosphate (pgp) was evaluated for genus level differentiation among strains of staphylococcus and micrococcus-sarcina spp.. hot saline extracts from whole cells of staphylococcus spp. strongly reacted with the pgp antiserum, whereas those of micrococcus-sarcina spp. did not. likewise, phenol-water extracts from whole cells of micrococcus-sarcina spp. were not reactive with the pgp antiserum, although the extracts of staphyl ...197897498
residue monitoring of food products of animal origin. 197896909
polypeptide antibiotic 26a from bacillus subtilis. ii. isolation and purification procedures.both the analytical and preparative methods by which the preparations of 26a bydrochloride salt with a high antibacterial activity and 20--30% recovery have been obtained from the fermentation fluids of bacillus subtilis are presented. on an industrial scale the antibiotic can be yielded by absorption of bioactivity on amberlite cg-50i column and precipitation with picric acid of crude substance from active elutes as adduct which was divided on equilibrated cm--cellulose and finally purified by ...197881596
tissue distribution and residues of benzylpenicillin and aminoglycoside antibiotics in emergency-slaughtered ruminants. 197875582
radioimmunoassay of neocarzinostatin, an antitumor protein.antibodies directed toward the antitumor protein neocarzinostatin (ncs) have been produced in a rabbit by immunization with a highly purified ncs preparation. the antiserum was monospecific and reversed the antibacterial activity of ncs against sarcina lutea. it cross-reacted with chemically modified derivatives of ncs and mitomalcin but failed to cross-react with macromomycin. a radioimmunoassay procedure has been developed utilizing the antiserum and a biologically active 125i-labeled derivati ...197663321
a new variety of chainia antibiotica showing antisarcina activity. 1979548525
determination of penicillin residues in milk - a comparison of two methods.two tests for penicillin residues in milk using the test organisms sarcina lutea and bacillus stearothermophilus var. calidolactis respectively are presented. the test methods are described, the results compared and the advantages of the latter test demonstrated.1979551209
[bacteriology of the atmosphere in microclimate tents].micro-climate tents (pulsed filtered air) are frequently used in burns departments. in the surgical and traumatological intensive care unit of the department of anaesthesia and intensive care of the henri mondor hospital, these tents are used in two types of indications: --placing of a tent over the head end of patients following tracheotomy with free spontaneous ventilation;--placing tents over irregular wounds. in these two categories of use, the aim is to isolate the zone under the tent by pr ...197944965
production of l-serine by sarcina albida.conditions for the production of microbial l-serine hydroxymethyltransferase and for the conversion of glycine to l-serine were studied. a number of microorganisms were screened for their abilities to form and accululate l-serine from glycine, and sarcina albida was selected as the best organism. enzyme activity in this organism as high as 0.12 u/ml could be produced in shaken cultures at 30 degrees c in a medium containing glucose, ammonium sulfate, glycine, yeast extract, and inorganic salts. ...197939497
determination of virginiamycin in feeds.virginiamycin was extracted from the feed by ethanol-ph 2.5 phosphate buffer (1 + 1). the ph during extraction was adjusted (when necessary) to between 4 and 5. sample dilutions and the standard dose response line were prepared to contain ethanol ph 6 phosphate buffer (2 + 8), and the test organism was sarcina lutea. three feeds (a poultry ration, a swine finishing ration, and a swine starter ration) showed virginiamycin recovery of 88.8--108.9% when standard solutions were added at concentratio ...197939060
the enzymatic conversion of l-histidine to urocanic acid by whole cells of micrococcus luteus immobilized on carbodiimide activated carboxymethylcellulose.whole cells of micrococcus luteus (formerly sarcina lutea atcc 9341) have been covalently linked to a carboxymethylcellulose support system, with the retention of histidine ammonia-lyase activity. the dependence of the rate of urocanic acid formation on ph, temperature, and added surfactant concentration was similar for the free and the immobilized cells. the immobilization procedure used is based on the carbodiimide activation of carboxymethylcellulose and has been optimized for the histidine a ...197715677
spontaneous deamidation of a protein antibiotic, neocarzinostatin, at weakly acidic ph. conversion to a homologous inactive preneocarzinostatin due to change of asparagine 83 to aspartic acid 83 accompanied by conformational and biological alterations.the amide content of neocarzinostatin (ncs), an antitumor protein, has been determined by analysing asparagine and glutamine in the pronase-aminopeptidase m digests of tetra-s-carboxymethyl-ncs and carboxyl-modified ncs (modified with a water-soluble carbodiimide and 14cglycine methyl ester). preneocarzinostatin (pre) was separated and purified from a crude ncs preparation by cm-cellulose column chromatography. pre was found to contain one mole less asparagine than ncs, and asparagine was deamid ...197714934
[relationship between adsorption of microorganisms and the stage of their development].bond strength during adsorption of microorganisms depends on their growth stages, as was found by light optical and scanning electron microscopy. the strenth of adsorption often decreases as a culture growth on the surface of an adsorbent. adsorption of microorganisms is an important ecological process. under favourable conditions, microorganisms grow at a high rate when they are firmly bound to a solid surface. after abundant growth which deteriorates their microenvironment, microorganisms deso ...1977329067
[fundamental and clinical investigation of clindamycin-2-phosphate in otorhinolaryngological field (author's transl)].some laboratory examinations were made with clindamycin-2-phosphate (cldm-2-phos.). the drug was applied clinically to several otorhinolaryngological infections and the good results were obtained as follows. 1) observing by biophotometer, the growth of staphbylococcus aureus fda 209p was inhibited well by the serum (diluted 10-fold) 30 minutes, 1, 2, 4 hours after 300 mg of cldm-2-phos. were injected intramuscularly. 2) serum concentration was determined by thin-layer method with sarcina lutea a ...1977264983
the bacteriological ecosystem of the skin of children in an african tropical envirovment (tanzania).using a standardized method for sampling, the aerobic microbial flora of the skin of ninety-five children in dar es salaam, tanzania, was studied qualitatively and quantitatively. the subjects comprised three groups; group a, thirty-seven normal infants (below the age of 1 year); group b, twenty-nine infants with various bacterial skin lesions; and group c, twenty-nine nursery school children between 4-5 and 7 years. the following observations were made: (i) the quantitative results indicated; ( ...1975239739
isolation and structure assignment of an antimicrobial principle from the volatile oil of nigella sativa l. seeds.refrigeration of the volatile oil of nigella sativa l. seeds eventuates in a crystalline substance. the chemical structure of the compound was drawn from its chemical behaviour, as well as from its uv, ir, pmr and mass spectral data. the compound was found to be thymohydroquinone: confirmation of the structure was established via the preparation of its corresponding diacetate ester. the compound was found to have high antimicrobial effect against gram positive microorganisms1975238225
[studies on the use of the se-(selective enterococcal) medium for routine diagnosis of enterococci]. 1977331699
chartreusin: production and microbiological assay.chartreusin was produced in the fermentation liquors of streptomyces chartreusis at peak concentrations of 200 to 300 mug/ml. the titers could be increased by 200 to 300% or more by incorporating d-fucose, a part of the chartreusin molecule, into the fermentation media. a microbiological assay with sarcina lutea could detect concentrations of the drug of 0.5 to 1.0 mug/ml.1977335963
the use of the natural binding of bacteria for the identification of human t- and b-lymphocyte subpopulations in blood smears. 1979378486
target substances of some antifungal agents in the cell membrane.copiamycin, an antifungal antibiotic, exhibits antimicrobial activity against a few bacteria in addition to a wide variety of fungi. the methanol extract of sarcina lutea, one of the most susceptible bacteria, was found to reverse the antimicrobial activity of copiamycin. the reversing activity was associated with the phospholipid fraction of the bacteria. the s. lutea phospholipids also reversed the activities of azalomycin f and miconazole, but not that of clotrimazole. the effects of authenti ...1978400824
stability of cephalosporins in horse serum.the antibiotic activity of cephalothin, cephaloridine, cephalexin, cephaloglycin, cefazolin, and cefamandole was determined after storage for up to 30 days in horse serum at -10 and 4 degrees c. cephalothin, cefamandole, cefazolin, and cephalexin were stable for at least 30 days at -10 degrees c, whereas cephaloridine lost 29% of its initial activity and cephaloglycin lost more than 50%. cefamandole, cefazolin, and cephalexin could only be stored for 3 days at 4 degrees c without significant los ...1977402105
identification of five human lymphocyte subpopulations by their differential binding of various strains of bacteria. 1977405106
metabolism of isoniazid & related compounds by microorganisms: isolation, characterization & growth of isoniazid-degrading organism. 1978750380
alkyl streptozotocin analogues with improved biological activities.alkyl 16alpha- and -beta-glycosides of a series of n3-alkyl homologues of streptozotocin were synthesized from glucosamine hydrochloride. these compounds, when tested against ascites sarcoma 180, ehrlich ascites carcinoma, or leukemia l1210, exhibited potent antitumor activities, and antibacterial and diabetogenic activities were eliminated. furthermore, the acute toxicities of these compounds were lower than that of streptozotocin. the methyl, ethyl, n-propyl, and n-butyl glycosides of streptoz ...1976133244
metabolism of nicotinic acid by sarcina sp. 1978753746
2-hydroxy-isonicotinic acid--an intermediate in metabolism of isonicotinic acid hydrazide & isonicotinic acid by sarcina. 1978753753
antibacterial and antitumor activity of some derivatives of ureidosuccinic acid.the effect of several derivatives of ureidosuccinic acid on the growth of escherichia coli 387, staphylococcus aureus strain 209 and its mutant uv-2, sarcina lutea, candida tropicalis and neurospora crossa 9863 as pre-screening systems for antitumor activity was studied. it was found that dihydrazide of d,l-ureidosuccinic acid (dhua) had a marked antibacterial activity. the inhibitory effect of dhua on n. crassa could not be removed by aspartic acid, ureidosuccinic acid, dihydroorotic acid, orot ...1976131912
rapid bioassay for clindamycin alone and in the presence of aminoglycoside antibiotics.a rapid bioassay for determination of concentrations of clindamycin in serum was developed with use of a strain of lancefield group b streptococcus (streptococcus agalactiae) that is uniformly resistant to aminoglycoside antibiotics, tetracycline, and polymyxin. an agar diffusion assay system was used that included the addition of patient's sera and three standard concentrations of clindamycin to 5-mm wells cut in the seeded agar. pretreatment of serum with penicillinase allowed measurement of c ...1977409783
comparison of the effects of three fluorocarbons on certain bacteria.three fluorocarbons were tested to determine their effect on bacterial growth. freon 11 and 21 in various concentrations had an inhibitory effect on selected test organisms, but freon 22 had no effect. both aerobic and anaerobic microorganisms, as well as gram-positive and gram-negative species, were included among the bacteria tested. freon 11 and 21 caused a similar response with freon 11 being more inhibitory to some species and freon 21 more inhibitory to others. inhibition was dependent on ...1975803403
anti-riboflavin activity of 8-o-alkyl derivatives of riboflavin in some gram-positive bacteria.two new 8-o-alkyl derivatives of riboflavin (rf), i.e., 8-methoxy- (mof), and 8-ethoxy-8-demethyl-d-riboflavin (eof), their tetraacetate, and the tetraacetate of 8-hydroxy-8-demethyl-d-riboflavin (hof) were synthesized. the anti-rf activity of mof, eof and hof was estimated from the ratio cr/ci, where ci is the concentration of test flavin added to the culture medium and cr is the minimum concentration of rf needed to restore the growth inhibition. their activity was also compared with that of r ...1979118241
dissociated resistance among cephalosporins.when disks containing cefuroxime (cxm) or hr 756 (756) were placed next to cefoxitin (fox) on plates inoculated with enterobacter resistant to fox, there was marked flattening of the zones of inhibition produced by cxm or 756, but colonies growing near the cxm or 756 disks were found to be fully susceptible to these drugs on retesting. in contrast, when a checkerboard titration with cxm or 756 and fox was done in broth, organisms growing in the presence of high concentrations of either of the fo ...1979464581
comparative pharmacokinetics of amoxicillin and ampicillin in infants and children.the pharmacokinetics of amoxicillin and ampicillin were studied in 24 infants and children. mean peak serum concentrations of 5.4 micrograms/ml in fasting and 3.2 micrograms/ml in nonfasting patients were observed after 15 mg/kg amoxicillin doses. area under the curve values and serum half-life values were similar in fasting and nonfasting patients. the pharmacokinetics of amoxicillin (15 mg/kg) were compared to those of ampicillin (25 mg/kg). peak serum concentrations, area under the curve valu ...1979492836
bacillus stearothermophilus disk assay for detection of residual penicillins in milk: collaborative study.a collaborative study was performed on a bacillus stearothermophilus paper disk method designed to detect residual levels of 4 antibiotic drugs in whole market milk. this method is a modification of an earlier procedure developed for the international dairy federation. whole milk samples spiked at low levels with ampicillin, cephapirin, cloxacillin, and penicillin g were sent frozen to 11 collaborating laboratories with instructions to assay them promptly according to the method provided. five o ...1979528474
immunochemical detection of n6-methyladenine in dna.antibodies to n6-methyladenosine were produced in rabbit by means of immunization with n6-methyladenosine coupled to bovine serum albumin via periodate oxidation. cross-reacting antibodies were removed by bovine serum albumin-sepharose and appropriate nucleoside-human serum albumin absorbents. nucleoside-specific antibodies were isolated by affinity chromatography of n6-methyladenosine-human serum albumin-sepharose. the specificity of the purified antibodies has been demonstrated by complement f ...1979534639
isonicotinic & 2-hydroxy-isonicotinic acid hydroxylases of sarcina sp. 1979540926
[clinical studies on clindamycin-2-phosphate in the field of surgery (author's transl)].twenty eight patients were treated with parenteral clindamycin-2-phosphate in the field of surgery, and good response was obtained in a series of superficial soft tissue infection, especially caused by staphylococci, with a daily dose of 300 mg. serum level and urinary excretion were also investigated in four healthy male volunteers.1977557118
the photoinactivation of the respiratory chain in sarcina lutea (micrococcus luteus) and protection by endogenous carotenoid. 1977594177
[new species of actinoplanes cyanea sp. nov. and its antagonistic properties].an actinomycete stain 1569 isolated from a siberian soil sample is described as a representative of a new species, designated as actinoplanes cyaneus sp. nov. the actinomycete cell wall contains meso-diaminopimelic acid, arabinose, xylose and a non-identified analogue of diaminopimelic acid. the actinomycete forms spherical sporangia with mobile spores. the aerial mycelium is absent. the isolate produces a soluble blue pigment on synthetic media. the pygment investigation showed that it belonged ...1977596849
microbial conversion of dl-2-amino-delta2-thiazoline-4-carboxylic acid to l-cysteine and l-cystine: screening of microorganisms and identification of products.microorganisms able to form l-cysteine from dl-2-amino-delta2-thiazoline-4-carboxylic acid (dl-atc), a chemical intermediate in the synthesis of dl-cysteine, were isolated from soil samples and classified as pseudomonas sp., pseudomonas cohaerens, p. desmolytica, and p. ovalis. thirteen l-cysteine-producing bacteria were also found in among 463 stock cultures representing 37 genera. these were achromobacter delmarvae. alcaligenes denitrificans, bacillus brevis, brevibacterium flavum, enterobacte ...1977596877
glutamine-selective membrane electrode that uses living bacterial cells.a novel bioselective membrane electrode for l-glutamine has been constructed by coupling living bacteria of the strain sarcina flava to a potentiometric ammonia gas sensor. tests in aqueous standards and human serum show that the electrode combines excellent sensitivity and selectivity with rapid response and a useful lifetime of at least 2 weeks.1978619467
[metabolism of cefatrizine (s-640p) in rat, rabbit, dog, monkey and human (author's transl)]. 1976817050
improved microbiological assay for penicillin residues in tissues and stability of residues under cooking procedures.the microbiological assay for penicillin residues was improved by using centrifugation to remove physical barriers to diffusion, a small buffer/meat extraction ratio, and a more sensitive 2-layer assay system. recoveries from muscle, kidney, and liver tissues ranged between 70.1 and 86.7% with measurable levels of 0.03--0.05 unit/g. by comparison, the food and drug-suggested methodology yielded recoveries of 45.9--54.0% and levels of detectability of 0.08--0.10 unit/g. cooking of hamburger, stea ...1978649548
[analysis of the germ content of the air in university lecture rooms (author's transl)].the microflora of the air in university lecture rooms was examined with the sedimentation method. for this purpose blood agar (20 degrees c and 37 degrees c), saline agar and endo plates were used. the germ counts identified correlate to the size and utilization of the rooms. the lowest germ count was determined in the room used for practical work, which was followed by the lecture room. the newly built lecture room contained the highest colony count. the floor ventilation furnishes an explanati ...1978654689
plasma, bone, hip capsule and drain fluid concentrations of ampicillin and flucloxacillin during total hip replacement after intravenous bolus injection of magnapen.1. a rapid intravenous bolus injection of 4.0 g magnapen (which contains 2.0 g of ampicillin and 2.0 g of flucloxacillin) was to seven patients undergoing total hip replacement immediatly before induction of general anesthesia. postoperatively the patients patients received 2.0 g magnapen by intramuscular injection every 6 h for up to 72 h until removal of the wound drains. 2. the plasma, bone, hip capsule and drain fluid concentrations of ampicillin and flucloxacillin were measured by a differe ...1978678390
improved microbiological assay for penicillin residues in milk and dairy products.the microbiological assay for penicillin residues in milk was improved. acidification of milk with phosphoric acid to ph 4.5, centrifugation to remove precipitated proteinaceous matter, adjustment of ph to 6.0, a second centrifugation, and the use of a 2% agar base layer resulted in the consistent measurement of 0.01 unit penicillin activity/ml milk. sarcina lutea was used as the assay organism. recoveries in milk averaged 94%, in nonfat dry milk 84%, in cottage cheese 97%, and in cream cheese 9 ...1978681264
[pharmacokinetics of rifampicin in gastrectomized persons (author's transl)].a laboratory method was developed whereby the presence of rifampicin in the blood is determined microbiologically by means of a diffusion test. the organism used was sarcina lutea. the tests established that the rifampicin levels in the blood of pylorogastrectomized persons were significantly higher then they were in a normal control group. the daily changes in the rifampicin levels were simulated and checked by analogue computer. the findings indicated that the increased absorption in gastrecto ...1978693453
the isolation and characterization of rubradirin b.rubradirin b, c40h33n3o15, was separated from other components of the rubradirin complex by chromatographic and crystallization procedures. the spectrum of antibacterial activity is similar to that of rubradirin, but the antibiotic is less active.1978711617
saprophytic bacterial flora in the sebaceous gland infundibulum in diabetes mellitus. 1978736608
[pharmacokinetics of turimycin]. 1978736714
experimental scleral buckling with a soft xerogel implant. i. properties of poly(hydroxyethyl acrylate) compared with gelatin and other swelling implants.scleral buckling can be obtained with insoluble hydrophilic polymers that are implanted in the dry state (xerogels, which then expand to a predetermined size by imbibing tissue fluids. absorbable gelatin, nonabsorbable poly(hydroxyethyl methacrylate) (phema), and poly(glyceryl methacrylate) (pgma), have been implanted as xerogels in scleral buckling procedures. gelatin, phema, and pgma are soft when hydrated, but are hard and brittle in the dehydrated state. however, poly(hydroxyethyl acrylate) ...1978745816
[physicochemical studies of pharmachem's tylosin].studied is the effect of high temperature, light, moisture, the type of the solvent and the reaction in the medium on the chemical stability and biological activity of the tylosine base, tylosine tartarate and tylosine phosphate, produced by the "pharmachim" state economic corporation. the evaluation of the effect is carried out by using the spectrophotometric (in the uv-region) and microbiological methods (diffusion in agar with sarcina lutea a. t. c. c. 7341 as a test microorganism). it is fou ...1978753031
respiratory activity as a determinant of radiation survival response.respiration is depressed in irradiated bacteria reaching a minimum level in most strains at 1-3 h after exposure when incubated in growth medium. since a delay in response is observed, direct action on respiratory enzymes is unlikely. the dosage response of respiration varies widely in the strains studied with do's from 15 kr for escherichia coli bs-1 to 1200 kr for micrococcus radiodurans. all strains exhibit two-component dosage-response curves. escherichia coli bs-1 uniquely possesses a posit ...1976788873
concentrations of erythromycin in serum and tonsil: comparison of the estolate and ethyl succinate suspensions.concentrations of erythromycin were measured in serum and tonsil from children who had received either the estolate or ethyl succinate suspension before surgery. the in vitro assay measured total erythromycin activity against a group a beta hemolytic streptococcus. levels of erythromycin in serum and tonsil after single and multiple doses of the estolate were significantly higher than those after administration of the ethyl succinate. the therapeutic implications of these findings are unknown.1976792404
evaluation of two simple assay methods for detecting antibiotic residues in chicken and pig muscle. 1975806569
bacterial carotenoids. liii. c50-carotenoids. 19. absolute configuration of sarcinaxanthin and sarcinaxanthin mono-beta-d-glucoside. isolation of sarcinaxanthin diglycoside. 1977855546
occurrence of indole in cells of extremely halophilic bacteria.the neutral lipids of several halophilic bacteria contain a non-isoprenoid compound which was identified by its chromatographic and spectroscopic properties as indole.1977871975
[optimal conditions for determing atp of microbial origin].endogenous luminescence of the luciferin-luciferase extract from firely tails was studied in determing low concentrations of atp. the optimum concentration of the the extract, corresponding to the minumum effect of endogenous luminescence, was found in the reaction medium. the solution of luciferin-luciferase was unstable at room temperature and upon dilution. the activity of luciferin-luciferse solutions did not change after freezing in liquid nitrogen with following thawing. the content of atp ...1977882012
absorption of phenethicillin from oral paediatric formulations. 1975806767
[residues of antimicrobial substances in the meat and organs of slaughter calves].using a modified gartside diffusion method (1960) the incidence of the residues of antimicrobial substances was followed in two categories of calves commercially slaughtered at the brno slaughter house. for testing the following three collection strains were used: b. subtilis (atcc 6633), b cereus, var. mycoides (atcc 11778), sarcina lutea (atcc 9341). in light weight calves samples of bile, liver, kidney, diaphragmatic and hind limb muscles, in heavy calves, due to technical reasons, only bile, ...1975807014
systematic difference in the methylation of ribosomal ribonucleic acid from gram-positive and gram-negative bacteria.a survey of gram-positive and gram-negative organisms was performed to compare the distributionof n6-methylated adenine. it was found that (i) all the gram-positive strains tested, staphylococcus aureus, sarcina lutea, bacillus stearothermophilus, bacillus subtilis, and bacillus megaterium, contain neither n6-monomethyl adenine (m6a) nor n6-dimethyladenine (m26a) in 23s ribosomal ribonucleic acid (rrna). in the case of s. aureus and streptococcus pyogenes, strains which are clinically resistant ...1975807565
synthesis and antibacterial and antifungal activities of 5-nitro-2-furfurylidene polyhalophenoxyacethydrazides viii.fifteen 5-nitro-2-furfurylidene phenoxyacethydrazides were synthesized, and the antibacterial and antifungal activities of the compounds prepared were determined against different microorganisms. the o-methoxy derivative was found to be the most active compound.1975807705
tissue distribution and residues of beta-lactam antibiotics in normal dairy cows.tissue residues and concentrations of benzylpenicillin, cloxacillin, ampicillin, amoxycillin, cephapirin, and cephacetrile were determined in normal dairy cows after parenteral administration of several forms of these drugs. assay methods included the sarcina lutea kidney test of van schothorst, the bacillus subtilis bga tests at ph 6.0 and 8.0, the escherichia coli test and a sarcina lutea test performed at ph 8.0 of the agar, and specific quantitative assay methods. the e. coli test method dem ...1977918925
chemistry of cephalosporin antibiotics. 28. preparation and biological activity of 3-(substituted)vinyl cephalosporins.3-(substituted)vinylcephem nuclei have been prepared by the reaction of 3-formylcephem derivatives with stabilized phosphoranes. appropriate synthetic steps allowed preparation of a series of 3-ethoxycarbonylvinyl- and 3-carboxyvinylcephem derivatives bearing a variety of 7-acylamino functions. the phenoxyacetyl and thiopheneacetyl derivatives of the 3-cyanovinylcephem nucleus were also prepared. although general gram-positive activity was comparable to cephalothin in many cases, against penicil ...1975808607
synthesis and antitumor activity of 2-deamino- and n2-(gamma-hydroxypropyl)actinomycin d.2-deamino- and n2-(gamma-hydroxypropyl)actinomycin d were synthesized by modification of the parent actinomycin d molecule at the 2 position of the phenoxazinone moiety. the common intermediate was 2-deamino-2-chloroactinomycin d. catalytic hydrogenation of this material afforded the 2-deamino derivative while treatment with gamma-hydroxypropylamine yielded the n2-(gamma-hydroxypropyl) derivative. these 2-substituted actinomycin d derivatives were less potent in microbiological assays than the p ...1975809581
the fate of bacteria in frozen red cells.units of blood were intentionally contaiminated with suspensions of either aerobacter aerogenes, escherichia coli, bacillus subtilis, enterobacter cloacae, pseudomonas aeruginosa, sarcina lutea, seratia marcesens, staphylococcus epidermidis, streptococcus faecalis, paracolabacterum aerogenoides (enterobacter hafriae), mima polymorpha or acinetobacter- calcoaceticus. when inoculation was made prior to glycerolization, the subsequent glycerolization, freezing, thawing, and deglycerolization result ...1976936269
[false-positive results obtained on examining slaughtered animals for the presence of antibiotic residues (author's transl)].as part of the examination of emergency-slaughtered animals for the presence of antibiotic residues, studies were done to see whether false-positive results would be obtained when the sarcina lutea kidney test and bacillus subtilis bga test were performed. when the s. lutea kidney test was positive in cattle, calves and swine, penicillin was invariably found to be present in those animals, the histories of which showed that they had not been given antibiotics. a syringe and an injected fluid con ...1975809859
[transfer of organisms during exchange of heat and moisture in air conditioning installations (author's transl)].with the exhaust air from ventilation and air conditioning installations escaping into the open, the heat content is also lost which fresh air from outside obtains at considerable expense of energy and technical equipment. the heat content, on the other hand, consists of about equal proportions of sensible heat and latent heat which is associated with the moisture content of the air. in order to regain the heat content of the escaping air so as to be able to use it again - and this is becoming i ...1975811009
[antibacterial activity of a lysozyme-like enzyme from staphylococci].a lysozyme-like enzyme isolated from the culture broth of staph. aureus 712 presented in its native state a lipoproteid complex. the lytic and antibacterial spectrum of the enzyme was similar to that of the egg albumin enzyme. however, the lipoproteid complex was somewhat superior to the egg albumin complex in its activity against micrococcus lysodeikticus, a number of gramnegative bacteria and staphylococci. out of the organisms studied microccus lysodeikticus, sarcina and bac. subtilis proved ...1975813563
penicillin tolerance in neisseria gonorrhoeae: evidence disallowing a penicillinase-mediated mechanism from a refined microbiological assay method.a microbiological assay method has been developed and applied to neisseria gonorrhoeae, for the purpose of detecting enzymatic deactivation of benzyl penicillin. calibration of the method, using strains of escherichia coli k-12 with previously reported penicillinase (ec 3.5.2.6.) activities, has shown that it is extremely sensitive and may be used in a quantitative manner. at the limit of sensitivity the test is able to detect penicillin breakdown in the order of 3 x 10(-3) mug in 48 h, which is ...1976814985
cephalexin concentration in the human ejaculate following oral and parenteral administration.cephalexin levels in the ejaculates of 31 infection-free volunteers have been determined following the administration of 1 g of antibiotic. in 16 subjects the cephalexin was administered by intramuscular injection and in 15 by mouth. in each case the volunteers were divided into groups of five each of which received the drug, 1, 2 or 4 hours before the collection of semen. antibiotic concentrations were determined microbiologically on agar plates using sarcina lutea atcc 9341. for selection purp ...1978646144
pharmacokinetic interpretation of erythromycin and tylosin activity in serum after intravenous administration of a single dose to cows.the distribution and elimination kinetics of erythromycin and tylosin, which are macrolide antibiotics, were studied in healthy cows. a single dose (12-5 mg/kg) of drug was administered as an intravenous bolus, and blood samples were collected at precisely timed intervals. the standard cylinder plate bioassay method using sarcina lutea as test organism was employed to determine antibiotic activity in the serum. the results suggested that these drugs are distributed in at least two kinetically di ...19761030820
[species makeup, biological activity and antibiotic sensitivity of the microbes isolated from patients with a surgical infection].specimens from 227 patients with purulent surgical infections of various localization were tested. the microbial growth was observed in 87.6 per cent of the patients, staphylococci being found in 80.8 per cent of them. the plasma coagulating properties were detected in the staphylococci from 84.2 per cent of the patients among the total number of the staphylococcal isolates. staphylococci were mostly isolated from the patients with osteomyelitis, infected wounds. monocultures of the microbes and ...1976818943
antibiotics and the aberdeen typhoid outbreak in 1964.this paper gives an abbreviated account of part of a research programme which followed the aberdeen typhoid outbreak in 1964. chloramphenicol, the main antibiotic used in treatment, was shown to have a minimum inhibitory concentration (mic) of between 5 and 2-5 mug./ml. for the s. typhi phage type 34 of the outbreak. the mic for methacycline was between 5 and 2-5 and 2 mug./ml. whereas the deep and shallow broth techniques used gave similar results with these antibiotics, the mic for ampicillin, ...19761063214
phagocytic capabilities of the pigment epithelium. 19761084282
[experimental study of an aerosol preparation containing gentamicin (gentasol)].composition of a suspension aerosol drug, gentasol for local application was developed. it contained gentamycin and prednisolon. the antimicrobial properties of the drug were studied in vitro. it was found that gentasol had a pronounced antimicrobiol effect and inhibited the growth of many microorganisms including cultures resistant to other antibiotics. the drug was active against ps. aeruginosa and proteus, which is an advantage of it as compared to other antibiotic aerosol drugs. the study sh ...1976818945
[persistance of penicillin residues in the calf organism after parenteral therapeutical administration of the preparation penicillin g, procaine inj ad usum vet].by means of two biological diffusion methods a penicillin content in various calf tissues was determined. for the injections prokain penicillin g spofa, inj. adusum vet., was used in a single dose of 25 000 i. u. kg-1 body mass. for the tests three collection strains -- b. subtilis (atcc 6633), sarcina lutea (atcc 9341), and micrococcus flavus (atcc 10240) were used. a quantitative determination of penicillin in the samples was accomplished by reading from the standard curves. sensitivities of t ...1976820048
[antibacterial activity and presence of mercury in 12 species of marine algae]. 1975820360
[drug residues in poultry (author's transl)].the following microbiological tests: the bacillus subtilis bga (bundes-gesundheitsamt) test and sarcina lutea test as well as the test bacteria bacillus stearothermophilus var. calidolactis and e. coli were used to examine whether drug residues were detectable in broiler chickens during and after treatment with various drugs. with the exception of the occidiostat esb3, residues were not found to be present using the two above tests. on the other hand, residues of a number of drugs were detected ...1976821173
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