| hyperlipidemia in renal failure: studies of plasma lipoproteins, hepatic triglyceride production, and tissue lipoprotein lipase in a chronically uremic rat moedl. | to determine whether hypertriglyceridemia in chronic renal failure resulted from altered production and/or removal, sprague-dawley rats in which chronic uremia had been induced by five-sixths nephrectomy were studied. in this model, basal plasma tg levels were higher than in controls (uremic 141 +/- 52 mg/100 ml; control 83 +/- 25; mean +/- sd; p less than 0.001) at 1 month after nephrectomy and remained significantly elevated throughout the 10-week duration of study. in the uremic animals hepat ... | 1978 | 22574 |
| [effect of human antigens on the biological properties of the causative agents of typhoid fever and paratyphoid b]. | | 1979 | 91281 |
| [studies on human alpha 1-microglobulin. iii. serum and urine alpha-microglobulin levels in normal individuals, serum alpha 1-microglobulin levels in various diseases, and the relationship between the serum alpha 1-microglobulin and other plasma proteins (author's transl)]. | | 1979 | 91690 |
| effect of ischaemia on muscle contraction in man [proceedings]. | | 1979 | 92943 |
| differential location of different types of intermediate-sized filaments in various tissues of the chicken embryo. | the location of constitutive proteins of different types of intermediate-sized (about 10 mm) filaments (cytokeratin, vimentin, desmin, brain filament protein) was examined in various tissues of 11--20 day chick embryos, using specific antibodies against the isolated proteins and immunofluorescence microscopy on frozen sections and on isolated serous membrane. the tissues studied which contained epithelia were small intestine, gizzard, esophagus, crop, liver, kidney, thymus, mesenteries, and epid ... | 1979 | 93557 |
| infectivity suppressing and virus-binding activities of a membrane material isolated from tobacco leaves. | tmv binding substance (r) was isolated from a tobacco leaf membrane fraction and was purified by extraction with organic solvents and by column chromatography. experimental results suggest that the binding of r with tmv results in inactivation of tmv. when tobacco leaves were inoculated with the r-tmv complex, it was found that the formation of polysome containing infecting viral rna was inhibited. model experiments showed that the mode of r-tmv adsorption to the membrane is different from that ... | 1979 | 94422 |
| an unstable donor-recipient dna complex in transformation of bacillus subtilis. | in re-extracted dna obtained shortly after uptake of transforming dna by bacillus subtilis, increased amounts of donor dna radioactivity banding at the position of donor-recipient dna complex (drc) are observed in cscl gradients, if the cells are irradiated with high doses of uv prior to reextraction of the dna. qualitatively, the same phenomenon is observed if lysates of transforming cells are irradiated. uv-irradiation of lysates of competent cells to which single-stranded dna is added after l ... | 1978 | 106232 |
| changes in lung ultrastructure following heterologous and homologous serum albumin infusion in the treatment of hemorrhagic shock. | the object of this study was to compare the ultrastructure pulmonary effects of the infusion of homologous and heterologous serum albumin solution in the treatment of hemorrhagic shock in baboons. adult baboons subjected to hemorrhagic shock were resuscitated with either baboon serum albumin, human serum albumin, or ringer's lactate solution. the lungs were fixed in vivo with potassium pyroantimony, a solution which produces electron dense interstitial precipitation of sodium. the lungs from ani ... | 1979 | 106780 |
| [radioautographic identification of dopaminergic axons in the rat cns. in vitro marking with tritiated dopamine or noradenaline]. | a new radioautographic technique for the visualization of central dopaminergic fibers is described. this technique is based upon in vitro uptake of triatiated norepinephrine or tritiated dopamine in the presence of monoamine oxidase inhibitor and specific uptake inhibitor in noradrenergic fibers. after a short step in glutaraldehyde, the use of osmium vapours as a fixative, instead of osmic acid immersion, allows a satisfactory retention of triatiated amines within dopaminergic fibers. | 1979 | 120791 |
| [homologous transplants in the middle ear (author's transl)]. | the original concept of tympanoplasty included besides the closure of the middle ear, the removal of pathological middle ear components leaving intact the functional and healthy components. the special conditions that prevail in the middle ear (aeration, absence of intrinsic trauma) allow the reconstruction of the middle ear mechanics to a great extent. animal experiments and clinical experience reveal good transplantation properties of denatured and preserved ear ossicles. they are partly repla ... | 1975 | 123985 |
| lysergic acid diethylamide: evidence for stimulation of pituitary dopamine receptors. | lysergic acid diethylamide (lsd), 0.05 mg/kg and 0.20 mg/kg, significantly decreased plasma prolactin (prl) levels in male rats. lsd, 0.20 mg/kg, also inhibits the increase in plasma prl levels produced by chlorpromazine (cpz), 5 mg/kg, and alpha-methyl-paratyrosine (ampt), 50 mg/kg, both of which interfere with dopaminergic inhibition of prl secretion. lsd was more potent than methysergide, a serotonin receptor blocker, in lowering plasma prl levels and more potent than apomorphine, a known dir ... | 1977 | 143671 |
| [phosphofructokinase from brewers' yeast (author's transl)]. | | 1977 | 148074 |
| differential rates of synthesis of glycerokinase and glycerophosphate dehydrogenase in neurospora crassa during induction. | | 1975 | 165778 |
| genetic control of the glp system in bacillus subtilis. | in pleiotropic negative glycerol utilization mutants (glppi mutants) of bacillus subitilis, glycerol kinase and sn-glycerol 3-phosphate (g3p) dehydrogenase are noninducible. glppi mutants also fail to take up exogenous [14c]g3p. to study the regulation of the glp system in b. subtilis phenotypically, glp+ revertants were isolated from glppi mutants. four classes of revertants were identified: phenotypically, wild type; r1 type, which contains an informational suppressor, r2 type, which produced ... | 1976 | 182672 |
| the effects of prostaglandins e1 and e2 on heart rate responses to cervical sympathetic nerve stimulation and gaglion stimulant drugs in the anaesthetized mouse [proceedings]. | | 1976 | 184870 |
| modification of striatal acetylcholine concentration by n6,o2 -dibutyryl adenosine 3',5' -monophosphate in rats injected with chlorpromazine. | | 1977 | 195173 |
| cyclophosphamide induced sensitivity against avian rna myeloblastosis virus in age-resistant hosts. | the effect of cyclophosphamide administration on survival of 4- to 7-week-old chickens as well as on induction of leukemia after avian myeloblastosis virus (amv) injection was studied. the drug treatment alone did not cause any neoplastic effect in the birds during 4 months of observation. immediate application of amv to cyclophosphamide-pretreated age-resistant chickens induced acute myeloblastic leukemia in about 80 per cent of test animals. the sensitivity of chickens against amv, induced by ... | 1977 | 201874 |
| the involvement of the fully oxidized state in cytochrome oxidase reaction with oxygen studied with the 655 and nm band as a probe. | | 1977 | 202488 |
| effects of prophylactic treatment with the methanol extraction residue fraction of tubercle bacilli (mer) on the development of rous sarcomas of chickens following challenge with the rous sarcoma virus. | three-month-old chickens were treated with the methanol extraction residue fraction of tubercle bacilli (mer) under different conditions, and subsequently challenged with living rous sarcoma virus. the birds developed progressively growing sarcomas following viral challenge. a substantial proportion of the hosts which had been pretreated with mer under optimal circumstances (38 to 58%) showed complete and long-lasting regression of the neoplasms, and the survival of many of the animals that did ... | 1978 | 204612 |
| synthesis and enzymatic activity of adenosine 3',5'-cyclic phosphate analogs. | | 1979 | 225047 |
| the characterization of phosphoseryl trna from lactating bovine mammary gland. | bd-cellulose and rpc-5 chromatography of trna isolated from lactating bovine mammary gland showed the presence of four seryl-trna isoacceptors. the species, trna iv ser, with the strongest affinity for bd-cellulose (required ethanol in the elution buffer) could be phosphorylated in the presence of serine, [gamma-32 p]-atp, seryl-trna synthetase and phosphotransferase activity from the same tissue. o-phosphoserine was identified as the 32p-labelled product after mild alkaline hydrolysis of this a ... | 1977 | 242796 |
| the diagnostic application of specific antiprocollagen sera. ii. analysis of liver biopsies. | frozen, unfixed sections of human liver biopsies from patients with acute, subchronic, and chronic hepatitis or fibrotic liver disease were studied in indirect immunofluorescence with specific antisera to type i and type iii procollagen. in early stages of both hepatitis and fibrotic liver disease, intralobular type iii collagen synthesis is increased. maximum values are reached years after the onset of disease. intralobular procollagen i content is not increased in the acute stage, but rises on ... | 1978 | 344227 |
| studies on some characteristics of hydrogen production by cell-free extracts of rumen anaerobic bacteria. | hydrogen production was studied in the following rumen anaerobes: bacteroides clostridiiformis, butyrivibrio fibrisolvens, enbacterium limosum, fusobacterium necrophorum, megasphaera elsdenii, ruminococcus albus, and ruminococcus flavefaciens. clostridium pasteurianum and escherichia coli were included for comparative purposes. hydrogen production from dithionite, dithionite-reduced methyl viologen, pyruvate, and formate was determined. all species tested produced hydrogen from dithionite-reduce ... | 1977 | 558042 |
| fermentation of cellulose by ruminococcus flavefaciens in the presence and absence of methanobacterium ruminantium. | the anaerobic cellulolytic rumen bacterium ruminococcus flavefaciens normally produces succinic acid as a major fermentation product together with acetic and formic acids, h2, and co2. when grown on cellulose and in the presence of the methanogenic rumen bacterium methanobacterium ruminantium, acetate was the major fermentation product; succinate was formed in small amounts; little formate was detected; h2 did not accumulate; and large amounts of ch4 were formed. m. ruminantium depends for growt ... | 1977 | 562131 |
| adhesion of bacteroides succinogenes in pure culture and in the presence of ruminococcus flavefaciens to cell walls in leaves of perennial ryegrass (lolium perenne). | bacteroides succinogenes and ruminococcus flavefaciens are two of the most important cellulolytic bacteria in the rumen. adhesion of b. succinogenes in pure culture, and in mixed culture with r. flavefaciens, to the various types of cell walls in sections of perennial ryegrass (lolium perenne l. cultivar s24) leaves was examined by transmission and scanning electron microscopy. b. succinogenes adhered to the cut edges of most plant cell walls except those of the meta- and protoxylem. it also adh ... | 1978 | 567035 |
| [periodicity of changes in halobacterium cultures]. | | 1975 | 1183309 |
| [non-propagated potentials in the electromyogram of mammalian ocular muscles]. | electromyograms of mammalian extraocular muscles were recorded by means of a coaxial electrode. besides normal extracellular spike potentials (1-2 msec duration), monophasic waves (with a decline lasting up to 7 msec) were recorded. as to the interpretation of these potential changes in terms of a potential drop that is produced by local currents flowing from the resting region of a fibre towards the active region consideration is given to two cases. first, a propagated active region (spike pote ... | 1975 | 1199599 |
| proceedings: facilitation of the tonic vibration reflex in the spinal cat by 5-hydroxytryptophan (5-htp). | | 1975 | 168362 |
| [surface changes of the pulmonary valve following pulmonary valve xenotransplantation in the descending aorta. an experimental study in dogs]. | upon implantation in the descending aorta, glutaraldehyde-conditioned pulmonary valves (n = 46) are immediately covered by a protein layer, followed by a fibrinous layer. two to thirty minutes later platelets, wbcs, macrophages, and microthrombi are incorporated into the valve's surface. after 3 weeks the first collagen fibers appear on the surface and slowly build a collagen layer that grows toward the free edge. these changes are very similar to the surface changes of bioprostheses implanted i ... | 1979 | 110559 |
| effects of glycerol on the growth, adhesion, and cellulolytic activity of rumen cellulolytic bacteria and anaerobic fungi. | the effect of glycerol on the growth, adhesion, and cellulolytic activity of two rumen cellulolytic bacterial species, ruminococcus flavefaciens and fibrobacter succinogenes subsp. succinogenes, and of an anaerobic fungal species, neocallimastix frontalis, was studied. at low concentrations (0.1-1%), glycerol had no effect on the growth, adhesion, and cellulolytic activity of the two bacterial species. however, at a concentration of 5%, it greatly inhibited their growth and cellulolytic activity ... | 1992 | 1368974 |
| substance p as neurogenic mediator of antidromic vasodilation and neurogenic plasma extravasation. | | 1979 | 93706 |
| quantitative succinate-dehydrogenase histochemistry. i. a methodological study on mammalian and fish muscle. | in enzyme histochemistry formazan production can be used as a measure for oxidative enzyme activity. the formazan deposits can be measured quantitatively per cell with a scanning and integrating microspectrophotometer. optimal conditions are described for the estimation of histochemical succinate dehydrogenase activity in sections of fish bodymusculature and mouse soleus and plantaris muscle. it is shown that when proper measuring conditions are chosen a ditetrazolium salt (tnbt) can be used in ... | 1979 | 93099 |
| response surface analysis of the effects of ph and dilution rate on ruminococcus flavefaciens fd-1 in cellulose-fed continuous culture. | the ruminal cellulolytic bacterium ruminococcus flavefaciens fd-1 was grown in cellulose-fed continuous culture with 20 different combinations of ph and dilution rate (d); the combinations were selected according to the physiological ph range of the organism (6.0 to 7.1) and growth rate of the organism on cellulose (0.017 to 0.10 h-1). a response surface analysis was used to characterize the effects of ph and d on the extent of cellulose consumption, growth yield, soluble sugar concentration, an ... | 1992 | 1514805 |
| [treatment of experimental staphylococcal endophthalmitis]. | | 1979 | 92092 |
| detection of a low-molecular-weight antigen on melanoma cells by a human antiserum in leukocyte-dependent antibody assays. | biochemical characterization of serologically detected human melanoma antigens was undertaken for the development of immunodiagnostic assays in melanoma. an antiserum from a human melanoma patient, which detected melanoma antigens expressed on a large proportion of different melanoma cells, was used in leucocyte-dependent cytotoxic antibody (lda) 51cr-release assays to monitor the purification of melanoma antigens in urea/acetate extracts of lactoperoxidase 125i-labelled melanoma cell membranes. ... | 1979 | 91379 |
| the development of mesosomes of staphylococcus aureus during autolysis. | mesosomes of staphylococcus aureus were examined morphologically under the electron microscope. three different methods of specimen preparation (thin section, freeze-fracture, and negative staining) were used to eliminate artifacts due to sample processing. mesosomes were rarely seen in intact cells but were quite distinct in autolysed cells incubated in 1.2m sucrose -0.33m acetate buffer (ph 5.8) at 25 degrees c. the phospholipid content increased by 20% of the control as autolysis proceeded. s ... | 1979 | 91308 |
| a survey of peptidase activity in rumen bacteria. | twenty-nine strains of 14 species of rumen bacteria were screened for their ability to hydrolyse ala2, ala5, glyarg-4-methoxy-2-naphthylamide (glyarg-mna) and leu-mna. several species, notably megasphaera elsdenii, were active against ala2, and a smaller number, including bacteroides ruminicola, butyrivibrio fibrisolvens, ruminococcus flavefaciens, lachnospira multipara and ruminobacter amylophilus, broke down ala5. streptococcus bovis had an exceptionally high leucine arylamidase activity. howe ... | 1991 | 1748877 |
| effect of increased static lung recoil on bronchial dimesions of excised lungs. | we measured bronchial diameters and lengths during static deflation and inflation in eight excised dog lobes before and after static lung recoil (pst(l)) had been significantly increased by cooling the lobe for 48 h at 4 degrees c and ventilating it for 3 h. in control lobes, bronchial diameters were the same at any volume even though pst(l) was different during inflation and deflation. these results agree with those of hughes et al. (j. appl. physiol. 32: 25-35, 1972). however, when pst(l) was ... | 1975 | 1176409 |
| sequence of a cellulase gene from the rumen anaerobe ruminococcus flavefaciens 17. | a cellulase gene (enda) was isolated from a library of ruminococcus flavefaciens strain 17 dna fragments inserted in puc13. the enda product showed activity against acid-swollen cellulose, carboxymethyl-cellulose, lichenan, cellopentaose and cellotetraose, but showed no activity against cellotriose or binding to avicel. nucleotide sequencing indicated an encoded product of 455 amino acids which showed significant sequence similarity (ranging from 56% to 61%) with three endoglucanases from rumino ... | 1991 | 1886616 |
| the role of sensitized macrophages in induction of specific cell mediated cytotoxicity. | | 1975 | 1082790 |
| primary lymphoma of the heart. prolonged survival with early systemic therapy in a patient. | primary lymphoma of the heart is an uncommon malignancy usually recognized at autopsy or fatal within a few weeks of diagnosis. recently, it was reported in patients with acquired immune deficiency syndrome. a patient with diffuse large cell lymphoma of the heart is reported who had chest pain and rapidly evolving cardiac arrhythmias. the human immune deficiency virus antibody test was negative. because of an aggressive diagnostic approach, therapy with cyclophosphamide, doxorubicin, vincristine ... | 1991 | 1913464 |
| degradation of wheat straw and alkaline hydrogen peroxide-treated wheat straw by ruminococcus albus 8 and ruminococcus flavefaciens fd-1. | degradation of wheat straw (ws) and alkaline hydrogen peroxide (ahp)-treated wheat straw (ahpws) by ruminococcus albus 8 and ruminococcus flavefaciens fd-1 was determined by measuring the growth (od600) of each bacterium and determining dm disappearance (dmd) of the substrate. complex medium and defined medium with or without the addition of phenylpropanoic acid (ppa) and phenylacetic acid (paa) were used. tubes were incubated at 39 degrees c for 8 d. both od600 and dmd indicated that ahpws was ... | 1991 | 2016208 |
| the hydrolysis of lucerne cell-wall monosaccharide components by monocultures or pair combinations of defined ruminal bacteria. | the defined ruminal bacterial strains fibrobacter succinogenes s85, ruminococcus flavefaciens fd1, ruminococcus albus 7, butyrivibrio fibrisolvens d1, and bacteroides ruminicola ga33 were grown, in monocultures or as combinations of pair strains, on isolated lucerne cell-walls (cw) as the sole carbohydrate substrate. fibrobacter succinogenes s85 was the dominant strain determining extent of cw hydrolysis in all combinations with s85. the hydrolysis of cellulose, xylan, hemicellulose side-sugars, ... | 1991 | 2030098 |
| cellulolytic cocci isolated from the cecum of guinea pigs (cavia porcellus). | five strains of anaerobic, gram-variable cellulolytic cocci, belonging to the genus ruminococcus, were isolated from the cecum of a guinea pig. they differed from most previously described strains of cellulolytic ruminococci as follows. (i) lactate was the major fermentation product; lesser amounts of formate and ethanol and a trace of succinate were also produced, along with an uptake of acetate. (ii) no growth occurred at 30 degrees c; however, good growth was observed at 38 and 45 degrees c, ... | 1977 | 879784 |
| expression of two xylanase genes from the rumen cellulolytic bacterium ruminococcus flavefaciens 17 cloned in puc13. | two distinct xylanase genes (designated xyna and xynb) were subcloned in puc13 from non-homologous restriction fragments of ruminococcus flavefaciens 17 dna originally isolated in lambda embl3. the products of the two genes showed similar ph optima for hydrolysis of oat spelt xylan (around 5.5) and had little or no activity against carboxymethylcellulose. trace activities against p-nitrophenyl (pnp) cellobioside and pnp-xyloside were detected in clones containing xyna, but not in one harbouring ... | 1991 | 2045775 |
| [the direct detection of sinus nodal potentials in man by means of the signal-averaging technique (author's transl)]. | in 13 patients in the age range of 34 to 70 years (mean: 53 +/- 10 years), during diagnostic his-bundle investigations, registrations from the sinus nodal area were analyzed with a signal-averaging computer. in 12 patients we were able to get reproducible per-p-potentials with a sino-atrial conduction interval between 56 and 227 ms. the normal sequence of the excitation process from the atrium to the av node together with the bundle of his and to the ventricles in all patients, and the reproduci ... | 1979 | 516847 |
| incidence and pathological features of spontaneous tumors in athymic nude mice. | systematic observation of 1141 nude mice (swiss background [corrected] strain) that received human tumor transplants revealed 24 spontaneous tumors, 18 of lymphoreticular origin and 6 pulmonary adenomas. spontaneous tumors were seen at an average age of 9.1 months, and 22 of the tumors were seen only in that fraction of our group (324 mice) surviving for 5 months or more (22 of 324 x 100 = 6.8%). transplantation of these tumors to other nude mice was successful in three of five cases. mice trans ... | 1979 | 427772 |
| rapid isolation of legionella pneumophila from seeded donor blood. | the laboratory isolation of legionella pneumophila from seeded donor blood, using the lysis-centrifugation technique, is described. time to pure culture isolate was 3 to 4 days. | 1979 | 387807 |
| the properties of forms of ruminococcus flavefaciens which differ in their ability to degrade cotton cellulose. | two mutant strains of ruminococcus flavefaciens strain 007 that differ in their ability to hydrolyse cotton fibres have been shown also to differ in their cell-surface topology, in that the cotton degrading form possessed larger and more protuberant cell surface structures. the strains had similar cmcase, cellobiosidase and beta-glucosidase activities. the results indicate the importance of cell-surface properties in cotton degradation by r. flavefaciens. | 1990 | 2283039 |
| an inducible proline transport system in candida albicans. | 1. when candida albicans cells were preincubated with proline or grown in the presence of proline as the sole nitrogen source they exhibited a rapid increase in the influx of proline (the inducible transport system). 2. the induction appeared to be specific for proline and also demonstrated in other candida species. 3. both the inducible and constitutive proline uptake systems exhibited similar characteristic features. 4. the nature of the inducer for proline uptake in c. albicans appeared to be ... | 1979 | 383150 |
| characterization and nucleotide sequence determination of a repeat element isolated from a 2,4,5-t degrading strain of pseudomonas cepacia. | pseudomonas cepacia strain ac1100, capable of growth on 2,4,5-trichlorophenoxyacetic acid (2,4,5-t), was mutated to the 2,4,5-t- strain pt88 by a cole1::tn5 chromosomal insertion. using cloned dna from the region flanking the insertion, a 1477-bp sequence (designated rs1100) was identified which was repeated several times on the wild-type chromosome and was also present on ac1100 plasmid dna. various chromosomal fragments containing this sequence were cloned and their nucleotide sequence was det ... | 1989 | 2546860 |
| [anatomical aspects of the use of nuclear magnetic resonance methods]. | | 1989 | 2686590 |
| [anatomical aspects of the use of nuclear magnetic resonance methods]. | | 1989 | 2686590 |
| rabbit mammary prolactin receptors. demonstration of a late puerperal increase in affinity. | crude receptor preparations of rabbit mammary gland were made by differential centrifugation and reacted with lactoperoxidase-iodinated ovine prolactin (oprl) in order to determine their binding characteristics. receptors prepared from the mammary glands of animals less than 4 days postpartum bound oprl with high affinity (ka = 3.50 x 10(9) m-1), in good agreement with previous results of other investigators. the binding capacity of these preparations was 107 +/- 16.3 fmol/mg of protein. in cont ... | 1978 | 203595 |
| a universal lipid exchange protein from rat hepatoma. | the postmicrosomal protein fraction from rat hepatoma 27 adjusted to ph 5.1 stimulates phospholipid exchange between rat liver microsomes and mitochondria with higher rates and in a less specific way than the corresponding fraction from rat liver. a phospholipid exchange protein has been purified to homogeneity from the hepatoma ph-5.1 supernatant by gel filtration on sephadex g-75 and ion-exchange chromatography on carboxymethylcellulose. the isolated protein had a molecular weight of 11200 as ... | 1978 | 203454 |
| transformation of human cells by temperature-sensitive mutants of simian virus-40. | conditions necessary for the establishment and maintenance of transformation of human cells by wild type and temperature-sensitive mutants of sv40 were examined. for both early and late mutants, the frequency of transformation was found to be up to 5-fold higher, and virus yield 100-fold lower, at 39 degrees than at 33 degrees. no such effect was observed with the wild type virus under the same conditions. this observation is apparently at variance with previously published work, but may be expl ... | 1978 | 202569 |
| in-vitro activity of fce 22101 against respiratory tract pathogens with reference to production of beta-lactamases. | fce 22101 is a new penem with broad antibacterial spectrum, excluding the pseudomonads, and has stability to many beta-lactamases. fce 22101 and imipenem were very potent against the bacteria studied, including beta-lactamase producing strains, which can be isolated from patients with respiratory tract infections (mic less than or equal to 8 mg/l). no strains were found to be resistant to fce 22101. fce 22101 was rapidly bactericidal and more stable to inactivation by beta-lactamases from branha ... | 1989 | 2786517 |
| fibrous histiocytoma of the tarsus. | a 21-year-old white man had an asymptomatic eyelid mass that had slowly enlarged for one year. on clinical examination the lesion was well circumscribed; it appeared subconjunctivally but provoked no conjunctival inflammation, and had a consistency suggestive of cartilage. microscopic examination showed it was matted and composed of elongated fibro-histiocytes and an evenly distributed population of xanthoma cells and touton giant cells. the lesion was easily removed by local excision because of ... | 1977 | 202162 |
| [therapy of aids]. | therapeutic strategies in the management of aids should aim at an inhibition of the replication and spread of hiv; the reconstitution of immune functions; an effective chemotherapy of opportunistic infections and tumors; and the prevention of new opportunistic infections. at present, these goals can be only partially achieved and where beneficial therapeutic results are obtained they are only transient in nature. antiretroviral therapy and chemotherapy of infections have, however, resulted in so ... | 1988 | 2849247 |
| maturation of evoked climbing fiber input to rat cerebellar purkinje cells (i.). | an analysis of evoked responses of purkinje cells in developing rat cerebellum to climbing fiber input was conducted to determine which identifying properties of this afferent system are established early in development and which specific features mature with age. rat pups at various ages were anesthetized with 0.5% halothane and unit recordings made with glass micropipettes. by the third postnatal day, electrical stimulation of the sensorimotor cortex and limbs at low stimulation rates (less th ... | 1977 | 195825 |
| particulate carbonic anhydrase in homogenates of human kidney. | about 2% of human kidney carbonic anhydrase (carbonate hydro-lyase, ec 4.2.1.1) has been found in particulate fractions. its distribution in the particulate fractions obtained by differential centrifugation suggests that it may be concentrated in the brush border. the particulate enzyme is like red cell carbonic anhydrace c in its susceptibility to inhibition by anions. particulate carbonic anhydrase is firmly bound to the membrane and is not released by incubation at ph 10.6 and 37 degrees c or ... | 1976 | 184830 |
| synthesis and anti-dna -virus activity of the 5'-monophosphate and the cyclic 3',5'-monophosphate of 9-(beta-d-xylofuranosyl) guanine. | 9-(beta-td-xylofuranosyl)guanine (xylo-g) was converted chemically to the 9-(beta-d-xylofuranosyl)guanine 5'-monophosphate (xylo-gmp) and 9-(beta-d-xylofuranosyl)guanine cyclic 3',5'-monophosphate (c-xylo-gmp). these compounds were tested against a variety of dna viruses in tissue culture in parallel with 9-(beta-d-arabinofuranosyl)adenine (ara-a). this evaluation revealed that xylo-g, xylo-gmp, and c-xylo-gmp were all moderately active but less effective than ara-a. when the four compounds were ... | 1976 | 184282 |
| previous adaptation to a high-protein diet protects against streptozotocin-induced inhibition of insulin release from isolated rat islets. | the streptozotocin (stz)-induced inhibition of insulin release by isolated islets was measured in islets of rats previously adapted to either a high-protein (hp) or a balanced control diet. islets from rats adapted to an hp diet showed decreased susceptibility to the inhibitory effect of stz on insulin release. the data suggest that the beta cells from rats adapted to a high-protein diet have an increased resistance to the deleterious effects of stz. these results are consistent with in vivo dat ... | 1985 | 2937500 |
| conditional lethal mutants of vesicular stomatitis virus. | | 1975 | 169102 |
| molecular cloning of rat prostate transglutaminase complementary dna. the major androgen-regulated protein dp1 of rat dorsal prostate and coagulating gland. | complementary dna (cdna) that codes for a major androgen-dependent secretory protein of rat coagulating gland and dorsal prostate, dorsal protein 1 (dp1), was isolated by molecular cloning. recombinant dp1 cdna clones were identified from a bacteriophage lambda gt11 rat coagulating gland expression library using an affinity purified polyclonal antibody. amino acid sequence deduced from dna contained sequences identical with several dp1 cyanogen bromide cleavage fragments. northern blot hybridiza ... | 1992 | 1352290 |
| u3 sequences from htlv-i and -ii ltrs confer px protein response to a murine leukemia virus ltr. | human t-cell leukemia virus (htlv) types i and ii are unusual among replication-competent retroviruses in that they contain a fourth gene (chi) necessary for replication. the chi gene product, p chi, transcriptionally transactivates the viral long repeat (ltr), and is thus a positive regulator. to investigate p chi transactivation, sequences from the u3 regions of the ltrs of htlv-i and -ii were inserted into the moloney murine leukemia virus (m-mulv) ltr by recombinant dna techniques. transient ... | 1987 | 3027896 |
| influence of dietary fiber on xylanolytic and cellulolytic bacteria of adult pigs. | xylanolytic and cellulolytic bacteria were enumerated over an 86-day period from fecal samples of 10 8-month-old gilts that were fed either a control or a 40% alfalfa meal (high-fiber) diet. fecal samples were collected from all pigs on days 0, 3, 5, 12, 25, 37, 58, and 86. overall, the numbers of xylanolytic bacteria producing greater than 5-mm-diameter zones of clearing on 0.24% xylan roll tube medium after 24 to 36 h of incubation were 1.6 x 10(8) and 4.2 x 10(8)/g (dry weight) of feces for t ... | 1987 | 3030194 |
| spontaneous interictal spiking in the awake kindled rat. | the spontaneous interictal spike (sis) noted in eeg recordings is a signal of the abnormally excitable behavior of neurons in an epileptic focus. this study provides a detailed, quantitative, temporal and spatial profile of sis in the amygdaloid kindled rat. the reproducible nature of the development and decline of sis in kindling provides a useful model for biochemical study of the mechanisms that initiate and regulate sis. | 1979 | 91486 |
| roles of hormonal and nutritional factors in the regulation of rat liver alcohol dehydrogenase activity and ethanol elimination rate in vivo. | fasting reduced the liver alcohol dehydrogenase (adh) activity by 51% (p less than 0.001). insulin, within 2 hr, increased the adh activity found in fasted animals by 28% (p less than 0.02). insulin administration failed to stimulate the reduced adh activity in diabetic rats. however, adh activity in the diabetic-fed rats decreased by 52-54% (p less than 0.001) compared to normal-fed rats regardless of whether they were meal-fed or refed the normal chow. glucagon blocked by 15% (p less than 0.02 ... | 1988 | 3044171 |
| purification and properties of nadp-dependent glutamate dehydrogenase from ruminococcus flavefaciens fd-1. | glutamate dehydrogenase (gdh) (l-glutamate:nadp+ oxidoreductase, deaminating, ec 1.4.1.4) from the cellulolytic ruminal bacterium ruminococcus flavefaciens has been purified and characterized. the native enzyme and subunit are 280 and 48 kda, respectively, suggesting that the native enzyme is a hexamer. the enzyme requires 0.5 m kcl for optimal activity and has a ph optimum of 6.9 to 7.0. the kms for ammonia, alpha-ketoglutarate, and glutamate are 19, 0.41, and 62 mm, respectively. the sigmoidal ... | 1992 | 1335719 |
| isolation and properties of a (1,3)-beta-d-glucanase from ruminococcus flavefaciens. | a (1,3)-beta-d-glucanase [(1,3)-beta-d-glucan-3-glucanohydrolase] from ruminococcus flavefaciens grown on milled filter paper was purified 3,700-fold (19% yield) and appeared as a single major protein and activity band upon polyacrylamide gel electrophoresis. the enzyme did not hydrolyze 1,6-beta linkages (pustulan) or 1,3-beta linkages in glucans with frequent 1,6-beta-linkage branch points (scleroglucan). curdlan and carboxymethylpachyman were hydrolyzed at 50% the rate of laminarin. the enzym ... | 1991 | 1903625 |
| cellobiose uptake and metabolism by ruminococcus flavefaciens. | the cellulolytic ruminal bacterium ruminococcus flavefaciens fd-1 utilizes cellobiose but not glucose as a substrate for growth. cellobiose uptake by r. flavefaciens fd-1 was measured under anaerobic conditions (n2), using [g-3h]cellobiose. the rate of cellobiose uptake for early- or late-log-phase cellobiose-grown cells was 9 nmol/min per mg of whole-cell protein. cellobiose uptake was inhibited by electron transport inhibitors, iron-reactive compounds, proton ionophores, sulfhydryl inhibitors, ... | 1991 | 2036021 |
| preservation of ruminal bacterium capsules by using lysine in the electron microscopy fixative. | ruminal bacteria from axenic cultures of ruminococcus flavefaciens fd1, butyrivibrio fibrisolvens 49, and bacterial types from the ruminal ecosystem that were fixed with 50 mm lysine (l-lysine hydrochloride) added to glutaraldehyde had better-preserved capsules and extracellular material than bacteria fixed without lysine. | 1990 | 2125818 |
| tetrahydrofolate and other growth requirements of certain strains of ruminococcus flavefaciens. | two strains of ruminococcus flavefaciens were studied. each grew in a chemically defined minimal medium containing: minerals; ammonium sulfate as a nitrogen source; amino acids as a nitrogen source, a growth promotant(s) or as both; cellobiose as an energy and carbon source; isobutyric acid, isovaleric acid, carbonic acid, and bicarbonate as additional carbon sources; and biotin, thiamine, and tetrahydrofolic acid as vitamins. tetrahydrofolic acid (5 ng/ml) served as a replacement for rumen flui ... | 1977 | 557955 |
| clonidine hyperphagia: neuroanatomic substrates and specific function. | recent studies have indicated that the alpha 2-noradrenergic agonist clonidine (clon), when peripherally and centrally administered, potentiates feeding in satiated rats in a manner similar to that observed following injection of norepinephrine (ne) into the hypothalamic paraventricular nucleus (pvn). the present experiments examined the effects of clon on meal patterns and macronutrient selection and compared these findings to earlier ne-stimulated feeding studies. administration of clon (25 nm ... | 1988 | 3147461 |
| nucleotide sequence of the cela gene encoding a cellodextrinase of ruminococcus flavefaciens fd-1. | the nucleotide sequence of a 3.6 kb dna fragment containing a cellodextrinase gene (cela) from ruminococcus flavefaciens fd-1 was determined. the gene was expressed from its own regulatory region in escherichia coli and a putative consensus promoter sequence was identified upstream of a ribosome binding site and a ttg start codon. the complete amino acid sequence of the cela enzyme (352 residues) was deduced and showed no significant homology to cellulases from other organisms. two lysozyme-type ... | 1990 | 2129544 |
| detection of glycoproteins separated by nondenaturing polyacrylamide gel electrophoresis using the periodic acid-schiff stain. | we observed that published methods for staining glycoproteins in sodium dodecyl sulfate-polyacrylamide gels did not stain glycoproteins separated by nondenaturing polyacrylamide gel electrophoresis. therefore, the periodic acid-schiff stain for glycoprotein was adapted for use with proteins analyzed by nondenaturing polyacrylamide gel electrophoresis. following nondenaturing polyacrylamide gel electrophoresis proteins were denatured in situ by incubation with aqueous 2% sodium dodecyl sulfate, 5 ... | 1990 | 2196831 |
| assessment of the endo-1,4-beta-glucanase components of ruminococcus flavefaciens fd-1. | the extracellular endo-1,4-beta-glucanase components of ruminococcus flavefaciens fd-1 were analyzed by high-performance liquid chromatography (hplc) by using deae ion-exchange, hydroxylapatite, and gel filtration chromatography and polyacrylamide gel electrophoresis (page). two endo-1,4-beta-glucanase peaks were resolved by deae-hplc and termed endoglucanases a and b. carboxymethyl cellulose (cmc) zymograms were achieved by enzyme separation using nondenaturing page followed by incubation of th ... | 1990 | 2383014 |
| molecular cloning of genes from ruminococcus flavefaciens encoding xylanase and beta(1-3,1-4)glucanase activities. | clones expressing activity against xylan or beta(1-3,1-4)glucan (lichenan) were isolated from a library of ruminococcus flavefaciens 17 dna made in bacteriophage lambda embl3. hybridization analyses indicated the recovery of four separate genes encoding xylanases that showed no detectable associated carboxylmethylcellulase activity. one of these genes was associated with clones that also expressed beta(1-3,1-4)glucanase and beta-xylosidase activities. | 1989 | 2757382 |
| improved assay for quantitating adherence of ruminal bacteria to cellulose. | a quantitative technique suitable for the determination of adherence of ruminal bacteria to cellulose was developed. this technique employs adherence of cells to cellulose disks and alleviates the problem of nonspecific cell entrapment within cellulose particles. by using this technique, it was demonstrated that the adherence of ruminococcus flavefaciens fd1 to cellulose was inhibited by formaldehyde, methylcellulose, and carboxymethyl cellulose. adherence was unaffected by acid hydrolysates of ... | 1989 | 2782879 |
| effect of the novel ionophore tetronasin (ici 139603) on ruminal microorganisms. | the antimicrobial activity of the novel ionophore tetronasin (formerly ici 139603) was compared with that of monensin for the growth of ruminal bacteria, protozoa, and an anaerobic fungus. the potency of tetronasin toward most bacteria and the fungus was an order of magnitude or more greater than that of monensin. lactobacillus casei was 55 times more sensitive to tetronasin than to monensin, indicating a potential role for tetronasin in reversing lactic acidosis. bacteria with a gram-positive u ... | 1988 | 3355139 |
| the dilation of arterioles in frog submaxillaris muscle caused by visible light. | transillumination of submaxillaris muscle with incandenscent lamp led to arteriolar dilation if the illuminance exceeded 1-2 x 10(3) lux or reached the time-mean value of 3 x 10(4) lux when a flash lamp was used (pulse duration 10 microseconds, repetition rate 50 s-1). the temperature increase within a light spot on the muscle did not exceed 0.1 degrees c, visible light being the only reason of the dilation of arterioles. at 6.3 x 10(4) lux (incandescent lamp, green filter) the latency of dilati ... | 1989 | 2786511 |
| monoclonal-antibody-mediated enzyme-linked immunosorbent assay for detection of reticuloendotheliosis viruses. | an enzyme-linked immunosorbent assay (elisa) is described for the detection of reticuloendotheliosis viruses (revs). the assay uses a mixture of monoclonal antibodies (mcas) prepared against a 62-kilodalton rev envelope glycoprotein (gp62) to capture antigen, rabbit anti-rev serum as detection antibody, and peroxidase-conjugated anti-rabbit igg as indicator antibody. the mcas were reactive with rev strain t, chick syncytial virus, and duck infectious anemia virus but unreactive against marek's d ... | 1988 | 2838016 |
| homologous catalytic domains in a rumen fungal xylanase: evidence for gene duplication and prokaryotic origin. | a cdna (xyna), encoding xylanase a (xyla), was isolated from a cdna library, derived from mrna extracted from the rumen anaerobic fungus, neocallimastix patriciarum. recombinant xyla, purified from escherichia coli harbouring xyna, had a m(r) of 53,000 and hydrolysed oat-spelt xylan to xylobiose and xylose. the enzyme did not hydrolyse any cellulosic substrates. the nucleotide sequence of xyna revealed a single open reading frame of 1821 bp coding for a protein of m(r) 66,192. the predicted prim ... | 1992 | 1406248 |
| synergism of rumen microbial hydrolases during degradation of plant polymers. | in isolated mixture of exocellular enzymes of rumen bacteria ruminococcus flavefaciens, butyrivibrio fibrisolvens and rumen fungus neocallimastix frontalis, specific activities of cellulases, hemicellulases and glycosidases were determined. the highest specific activities were shown mostly for proteins of n. frontalis. | 1988 | 3397009 |
| effects of potassium ion concentrations on the antimicrobial activities of ionophores against ruminal anaerobes. | the antimicrobial activities of monensin and lasalocid against representative strains of ruminal bacteria were evaluated in medium containing three different concentrations of potassium (1.3, 7.9, or 23.3 mm). the growth of eubacterium ruminantium was inhibited by low concentrations of ionophores (less than or equal to 0.16 mg/liter), while the strain of streptococcus bovis tested was resistant to high concentrations of ionophores (40 mg/liter) at all potassium concentrations tested. the mics of ... | 1987 | 3426214 |
| degradation of maize stem by two rumen fungal species, piromyces communis and caecomyces communis, in pure cultures or in association with cellulolytic bacteria. | two species of rumen fungi, piromyces (piromonas) communis fl and caecomyces (sphaeromonas) communis fg10, were cultured alone or in association with the cellulolytic bacteria ruminococcus flavefaciens or fibrobacter succinogenes on maize stem. a kinetic study of the degradation of the substrate was then made. after 48 h of culture, all non-lignified tissues observed by scanning electron microscopy disappeared with p communis and degradation was as complete as that observed in the rumen. in cont ... | 1992 | 1418394 |
| partial characterization of a dna restriction endonuclease from ruminococcus flavefaciens fd-1 and its inhibition by site-specific adenine methylation. | the principal dna restriction-modification system of the cellulolytic ruminal bacterium ruminococcus flavefaciens fd-1 is described. the restriction endonuclease rflfi could be separated from cell extracts by phosphocellulose and heparin-sepharose chromatography. restriction enzyme digests utilizing rflfi alone or in combination with sali, a restriction enzyme isolated from streptomyces albus g, showed that the dna sequence recognized by rflfi either overlapped or was the same as that recognized ... | 1992 | 1539994 |
| a bifunctional xylanase encoded by the xyna gene of the rumen cellulolytic bacterium ruminococcus flavefaciens 17 comprises two dissimilar domains linked by an asparagine/glutamine-rich sequence. | the nucleotide sequence of the xyna gene of ruminococcus flavefaciens 17 was determined and found to consist of a 2862bp open reading frame beginning with a ttg start codon. the predicted product, xyla, consisted of distinct amino-terminal (a) and carboxy terminal (c) domains (248 amino acids, including a putative signal sequence, and 332 amino acids, respectively) linked by a repetitive sequence (b, 374 amino acids) extraordinarily rich in asparagine (45%) and glutamine (26%) residues. domains ... | 1992 | 1584021 |
| microtubule proteins in neuronal differentiation. | | 1986 | 3510808 |
| a nuclear marker for mammalian cells and its use with intracerebral transplants. | the hoechst dye staining method has been successfully applied to the central nervous system in mammals and its use has been demonstrated in intracerebral transplantation. the technique is rapid, simple and based on intrinsic nuclear properties. it was found to be permanent and valid whatever the animal strains or ages, allowing the distinction of rat cells from those of mouse, studied either separately or in a cross-transplantation model. it permitted the detection of grafted cells in the area o ... | 1991 | 1716157 |
| role of glia in k+ and ph homeostasis in the neonatal rat spinal cord. | stimulation-evoked transient changes in extracellular potassium ([k+]e) and ph (phe) were studied in the neonatal rat spinal cords isolated from 3-13-day-old pups. in unstimulated pups the [k+]e baseline was elevated and phe was more acid than that in ringer's solution (3.5 mm k+, ph 7.3-7.35). the [k+]e and phe in 3-6-day-old pups was 3.91 +/- 0.12 mm and phe 7.19 +/- 0.01, respectively, while in 10-13-day-old pups it was 4.35 +/- 0.15 mm and 7.11 +/- 0.01, respectively. the [k+]e changes evoke ... | 1991 | 1828787 |
| purification and characterization of an exo-beta-1,4-glucanase from ruminococcus flavefaciens fd-1. | an exo-beta-1,4-glucanase (exo a) from ruminococcus flavefaciens fd-1 was purified to homogeneity and characterized. enzyme activity was monitored during purification by using the substrate p-nitrophenyl-beta-d-cellobioside (npc). over 85% of the npc activity was found to be extracellular once the filter paper was degraded (7 days). culture supernatant was harvested, and the protein was concentrated by ultrafiltration. the retentate (greater than or equal to 300,000 mr), containing most of the a ... | 1987 | 3115960 |
| fermentation of xylans by butyrivibrio fibrisolvens and other ruminal bacteria. | the ability of butyrivibrio fibrisolvens and other ruminal bacteria (6 species, 18 strains) to ferment a crude xylan from wheat straw or to ferment xylans from larchwood or oat spelts was studied. liquid cultures were monitored for carbohydrate utilization, cell growth (protein), and fermentation acid production. b. fibrisolvens 49, h17c, actf2, and d1 grew almost as well on one or more of the xylans as they did on cellobiose-maltose. b. fibrisolvens 12, r28, a38, x10c34, ard22a, and x6c61 exhib ... | 1987 | 3124741 |
| methylcellulose inhibition of exo-beta-1,4-glucanase a from ruminococcus flavefaciens fd-1. | a homogeneous preparation of exo-beta-1,4-glucanase a from ruminococcus flavefaciens fd-1 was competitively inhibited by low concentrations (less than 3 mm) of methylcellulose. the enzyme was also sensitive to the surfactant properties of methylcellulose at high methylcellulose concentrations. | 1988 | 3137869 |
| a novel technique for measuring hypothalamic and pituitary hormone secretion rates from collection of pituitary venous effluent in the normal horse. | we have described a novel technique for collecting pituitary venous effluent in the horse by placing a cannula in the intercavernous sinus close to the outlet of the pituitary veins using a venous pathway unique to equids. cannula placement and blood collection are carried out painlessly in fully conscious, ambulatory, unstressed animals. there is no interference to hypothalamic, pituitary or target organ function. the blood collected contains readily measurable concentrations of gonadotrophin-r ... | 1987 | 3585231 |
| alteration of lactase and aminopeptidase n expression in porcine enterocytes by energy intake and environment. | the influence of nutrition, environmental temperature and time of feeding on lactase and aminopeptidase n activities in enterocytes of young pigs has been investigated. animals were kept in the cold (10 degrees c) or warm (35 degrees c) and given either a high or low energy intake. in animals from the cold, lactase activity as determined by scanning microdensitometry was significantly lower at 4 h after a meal than it was in animals from the warm. at 6 h, the activities were similar in all group ... | 1986 | 2876455 |
| opal suppressor phosphoseryl-trna is not a substrate of phosphoserine aminotransferase. | a proposal of the role of animal opal suppressor phosphoseryl (ps)-trna is that ps-trna plays a role as an intermediate in the metabolic pathway from 3-phosphoglycerate to glycine. the labeled [32p]phospho[3h]seryl-trna was prepared and used as a substrate in the reaction of bovine brain ps aminotransferase (ec 2.6.1.52) in the presence of alpha-ketoglutarate. by analysis of the reaction product, no amount of 3-phosphohydroxypyruvate was found, even though phosphohydroxypyruvate was noted in the ... | 1986 | 3645547 |
| autonomic hyperactivity on diazepam withdrawal in rats. | | 1987 | 3666805 |
| the postprephenate biochemical pathways to phenylalanine and tyrosine: an overview. | | 1987 | 2955202 |