| survival of anaerobic bacteria during transportation. 1. experimental investigations on the effect of evacuation of atmospheric air by flushing with carbon dioxide and nitrogen. | the effect of evacuation of atmospheric air during transportation on recovery of anaerobic bacteria was investigated. evacuation of atmospheric air from glass tubes by flushing with pure carbon dioxide lowered the content of oxygen to about 0.4 per cent. three b. fragilis strains and one strain of fusobacterium mortiferum and of peptostreptococcus anaerobius were investigated. bacterial recovery was determined one hour and 24 hours after evacuation of atmospheric air by pure carbon dioxide and p ... | 1976 | 3089 |
| bacterial interference by oropharynegeal and clinical isolates of anaerobic bacteria. | anaerobic isolates were tested for bacterial inhibitory activity. of 144 isolates, 102 were from oropharynegeal washings, and 42 were from clinical specimens. thirteen facultative bacterial species (seven members of the enterobacteriaceae and six species of gram-positive cocci) were used as indicators of inhibition. eleven anaerobic species were isolated from oral secretions. all isolates of bacteroides melaninogenicus, the most commonly recovered species, consistently inhibited several species ... | 1976 | 10336 |
| hydrogen peroxide and superoxide radical formation in anaerobic broth media exposed to atmospheric oxygen. | fourteen different broth media were autoclaved under anaerobic conditions and then exposed to atmospheric oxygen. the hydrogen peroxide and superoxide radical formation as well as the bactericidal effect of the media were studied. the rate of killing of peptostreptococcus anaerobius vpi 4330-1 was high in media that rapidly autoxidized and accumulated hydrogen peroxide. in actinomyces broth (bbl), 50% of the cells were killed within 2 min, and in brewer thioglycolate medium (difco), 50% were kil ... | 1978 | 29560 |
| differentiation of several isolates of peptococcus magnus by counterimmunoelectrophoresis. | uniform sonicated suspensions of three clinical isolates of peptococcus magnus that were morphologically, chromatographically, and biochemically identical were used to produce hyperimmune antisera in rabbits. similar hyperimmune serum was prepared to a single strain of peptostreptococcus anaerobius. when these antisera and antigens were tested by counterimmunoelectrophoresis, the three isolates of peptococcus magnus were immunologically distinct. none of the antisera to peptococcus magnus formed ... | 1977 | 68929 |
| the allergenic structure of allergen m from cod. iii. studies on the antigenicity of long-sequence peptides. | fragments tm 1 (75 amino acid residues) and tm 2 (38 amino acid residues), and 3 other polypeptides (range 16-25 amino acid residues) of allergen m from cod were shown to be active in rabbit igg-mediated reactions. the same peptides were previously found to possess reactivity in ige-mediated reactions, thus suggesting a structural relationship between their antigenic and allergenic determinants. | 1979 | 84803 |
| localization of nors in chromosomes of mouse cell lines by a combined 33258-hoechst and ag-staining technique. | | 1979 | 84805 |
| x-linked immunocompetence and epstein-barr virus oncogenesis. | | 1979 | 86133 |
| [intratypical antigenic differentiation of poliovirus strains circulating in mali by means of cross-adsorbed sera]. | the intratypic antigenic differentiation of poliovirus strains isolated from sick or healthy children in mali in 1975-1976 was performed. the method is based on the use of cross-adsorbed sera in the neutralization tests in which the virus is titrated by the plaque procedure or by the cytopathic effect. all the examined strains of serologic types i, ii, and iii were distinguished by the antigenic structure from the vaccine strains and were classified as "wild". quantitative differences in the ant ... | 1978 | 86237 |
| extractable antigen shared by peptostreptococcus anaerobius strains. | extracts from several species of gram-positive cocci were prepared by a modification of the rantz-randall autoclave method and tested for reactions with rabbit anti-peptostreptococcus anaerobius (atcc 27337 and vpi 5737) sera in a capillary precipitin test. antigen preparations from two reference strains of p. anaerobius (atcc 27337 and vpi 5737) and six clinical isolates of p. anaerobius reacted with the p. anaerobius antisera. these extracts formed a line of identity by immunodiffusion and dis ... | 1979 | 88461 |
| [the effects of anti-androgenic agents on metabolism of testosterone; (ii) effects of various drugs on hepatic testosterone 5 alpha- and 5 beta-reductase and its regulating factor (author's transl)]. | | 1979 | 89050 |
| strain-specific antigens in rhizobium leguminosarum. | fifty strains of rhizobium leguminosarum, isolated from five species of host plant (pisum sativum, p. arvense, vicia sativa, v. faba, and a lathyrus sp.) were examined for the presence of strain-specific somatic antigens by immune-diffusions against 13 antisera. thirty eight strains (76 per cent) were found to belong to the same sero-group and were serologically indistinguishable from each other, but four of these strains also exhibited non-reciprocal cross reactivity with other antisera. in con ... | 1979 | 89766 |
| distribution of myosin isoenzymes among skeletal muscle fiber types. | using an immunocytochemical approach, we have demonstrated a preferential distribution of myosin isoenzymes with respect to the pattern of fiber types in skeletal muscles of the rat. in an earlier study, we had shown that fluorescein-labeled antibody against "white" myosin from the chicken pectoralis stained all the white, intermediate and about half the red fibers of the rat diaphragm, a fast-twitch muscle (gauthier and lowey, 1977). we have now extended this study to include antibodies prepare ... | 1979 | 90047 |
| polyribonucleotide inhibition of ribonucleic acid directed deoxyribonucleic acid polymerase of mouse mammary tumor (type b) virus and simian sarcoma (type c) virus. | | 1979 | 90331 |
| the elephant man. | | 1979 | 90342 |
| spontaneous interictal spiking in the awake kindled rat. | the spontaneous interictal spike (sis) noted in eeg recordings is a signal of the abnormally excitable behavior of neurons in an epileptic focus. this study provides a detailed, quantitative, temporal and spatial profile of sis in the amygdaloid kindled rat. the reproducible nature of the development and decline of sis in kindling provides a useful model for biochemical study of the mechanisms that initiate and regulate sis. | 1979 | 91486 |
| binding of purified, soluble major histocompatibility complex polypeptide chains onto isolated t-cell receptors. i. reactivity against allo- and self-determinants. | in this study, we tried to get information about the fine antigen-binding ability of purified, soluble, idiotype-positive t-cell receptor molecules. lewis anti-da t-cell receptors were purified from normal lewis serum by the use of anti-idiotypic immunosorbent and sodium dodecyl sulfate-polyacrylamide gel, and were coupled to cyanogen bromide-activated sepharose 4b. in parallel, lewis anti-da, lewis anti-bn, and da anti-lewis alloantibody immunosorbents were prepared. the major ag-b chain (44,00 ... | 1979 | 91653 |
| bovine thyroxine-binding globulin. purification and comparison of molecular weight and amino acid composition with human thyroxine-binding globulin. | bovine and human thyroxine-binding globulin were purified from serum by a three-step purification procedure which comprised affinity chromatography consecutively on thyroxine- and concanavalin a--sepharose and finally preparative polyacrylamide gel electrophoresis. the molecular weights of the two proteins were similar (54 000) as well as their carbohydrate contents while some differences in amino acid composition were found. rabbit antiserum against bovine thyroxine-binding globulin reacted wit ... | 1978 | 96867 |
| [effect of polychlorinated biphenyls in the rat: ii.--preliminary report on metabolic alterations (author's transl)]. | rats were fed with a diet containing 10 and 100 ppm (wet weight) of phenoclor dpr for 4 weeks in order to evaluate the toxicity of a french pcb mixture. the influence of pcb was studied by determining the liver weights, the hepatic and muscular proteins, glycogen and humidity content as well as hematocrite, glucose, proteins and lipids in the plasma, and the lipidic contents of the liver and the carcass. there was a significant increase in both liver to body weight ratios and liver lipid and pro ... | 1978 | 105425 |
| multiple sclerosis: immunochemical studies on the demyelinating serum factor. | sera from patients with multiple sclerosis (ms) frequently produce demyelination of central nervous system tissue cultures. the nature of the factors responsible for demyelination is not as yet clearly established. however, several authors previously reported, in in vivo and in vitro models, demyelinating activity in igg fractions isolated from sera and cerebrospinal fluid of ms patients14,42,48. we found the demyelinating activity of ms sera to be extremely labile to conventional biochemical tr ... | 1979 | 105780 |
| an unstable donor-recipient dna complex in transformation of bacillus subtilis. | in re-extracted dna obtained shortly after uptake of transforming dna by bacillus subtilis, increased amounts of donor dna radioactivity banding at the position of donor-recipient dna complex (drc) are observed in cscl gradients, if the cells are irradiated with high doses of uv prior to reextraction of the dna. qualitatively, the same phenomenon is observed if lysates of transforming cells are irradiated. uv-irradiation of lysates of competent cells to which single-stranded dna is added after l ... | 1978 | 106232 |
| the presence of antibody-coated anaerobic bacteria in asymptomatic bacteriuria during pregnancy. | quantitative anaerobic culture of urine samples obtained from 593 pregnant women by suprapubic bladder aspiration was performed to establish the involvement of anaerobic bacteria in asymptomatic urinary tract infections. the fluorescent antibody (fa) test was applied to the sediments of bladder aspirates to determine the site of infection. anaerobic bacteriuria (greater than or equal to 10(4) microorganisms/ml of urine) was found in 34 patients, of whom five were fa-positive. these anaerobes wer ... | 1979 | 118996 |
| [letter: study of anthropometric characteristics and of dermatoglyphics in the parents of children with 21 trisomy]. | | 1975 | 126416 |
| [pseudomonas aeruginosa--the causative agent of infection in birds]. | pseudomonas aeruginosa was isolated from the viscera of diseased and dead birds (young and adult chickens) as well as from 9 poultry houses on seven farms. its typing was performed on the basis of the blue-green coloration of the cultures, their hemolytic activity, the pleasant odour of lime-tree, the growth in culturing at 42 degrees c, the presence of the pyocianin pigment, and the positive results of the cytochromoxidase test after gaby and hadley and the biochemical test after kovacs. the pa ... | 1975 | 127412 |
| biologic activity of soluble collagen-anticollagen immune complexes as demonstrated by passive cutaneous anaphylaxis. | collagen-anticollagen immune complexes were prepared by gel filtration. complex formation was chemically and serologically demonstrated. complexes eluting in gammam- and gammag-peaks of antigen-antiserum mixture showed strong positive reactions in pca experiments performed with 20 guinea pigs. control preparations of gammam- and gammag-peaks of antiserum devoid of antigen gave significantly lower pca-response. the positive reaction of control preparations can be explained in part by a certain de ... | 1975 | 127469 |
| effect of dexamethasone on electrolyte transport in the large intestine of the rat. | | 1978 | 149689 |
| a nuclear mutation conferring aurovertin resistance to yeast mitochondrial adenosine triphosphatase. | a single gene nuclear yeast mutant was isolated whose mitochondrial f1-atpase was resistant to the specific f1 inhibitor aurovertin. the mutant enzyme was not cross-resistant to other f1 inhibitors. the binding of aurovertin to f1 and to the two largest f1 subunits (alpha and beta) was measured by enhancement of aurovertin fluorescence. aurovertin bound to wild type f1-atpase and to its monomeric beta subunit with about the same binding constant. it failed to bind to wild type alpha subunit or t ... | 1979 | 153911 |
| conditional lethal mutants of vesicular stomatitis virus. | | 1975 | 169102 |
| cyclic nucleotide phosphodiesterases in uterine development. | activities of cyclic nucleotide phosphodiesterase were studied in rat uterus as a function of age, dna and protein content. linear kinetics were observed for uterine homogenate cyclic gmp (cgmp) phosphodiesterase activity, but anomalous double-reciprocal plots, suggestive of multiple enzyme forms, were observed for cyclic amp (camp) hydrolysis, camp phosphodiesterase was therefore measured at high and low substrate concentrations, 200 mum and 0.25 mum camp, respectively, to approximate multiple ... | 1975 | 172392 |
| lysis of tumor cells by antibody and complement. vi. enhanced killing of enzyme-pretreated tumor cells. | the ascites form of a chemically induced guinea pig hepatoma, line-10, was resistant to killing in vitro by xenogeneic antibody and guinea pig complement. pretreatment of line-10 cells with certain proteolytic enzymes rendered tham susceptible to the killing action of antibody and guinea pig complement. the effects of enzyme pretreatment were dependent on enzyme concentration, temperature, and could be blocked by addition of competitive or non-competitive inhibitors. the effect of the enzyme tre ... | 1976 | 176270 |
| steroid production by quartered and capsular rat adrenal gland in response to haemorrhage. | 60 min after rapid bleeding (1.5--2.0 per cent of b. w.) both aldosterone and corticosterone production rate by quartered rat adrenals were found to be elevated. however, no difference was observed in the rate of aldosterone and corticosterone production by capsular adrenals of sham operated and hypovolaemic rats. corticosterone production rate by decapsulated adrenals was much more higher after haemorrhage than in the control group. the same alterations could be observed incubating adrenal tiss ... | 1975 | 179800 |
| effectiveness of orally administered bordetella pertussis vaccine in mice. | oral administration of killed bordetella pertussis organisms to mice results in increased resistance to an intracerebral infection with virulent b. pertussis cells. the rate of survival is dependent on the dose of antigen. but besides specific systemic immunity, which is persistent over a long period, also transient non-specific resistance is increased. these effects are evidently induced without penetration of bacterial substances into the circulation. | 1976 | 182602 |
| evaluation of the sodium polyanethol sulfonate disk test for the identification of peptostreptococcus anaerobius. | the previously reported sodium polyanethol sulfonate disk test for the identification of peptostreptococcus anaerobius (graves et al., 1974) was evaluated, with modifications. three bands of brucella agar, three inoculum sizes, and two inoculum sources were compared. nine stock cultures of p. anaerobius (eight normal flora isolates and atcc 27337) and 16 fresh clinical isolates were used. all cultures of p. anaerobius showed inhibition zones of 12 to 30 mm in diameter, regardless of test conditi ... | 1976 | 185236 |
| gelatin neutralization of the inhibitory effect of sodium polyanethol sulfonate on neisseria meningitidis in blood culture media. | the inhibitory effect of sodium polyanethol sulfonate (0.05%) upon growth of neisseria meningitidis was found to be neutralized by adding gelatin (l.1%) to the growth medium. the neutralizing effect was demonstrated in solid medium, as well as in nutrient broth for blood cultures. the findings parallel those of wilkins and west (6) regarding gelatin neutralization of the inhibitory effect of sodium polyanethol sulfonate on peptostreptococcus anaerobius. | 1977 | 195978 |
| dna repair processes in germ cells demonstrated in ejaculated sperms of rabbits treated with methyl methane sulfonate. | male rabbits were treated with a single i.v. injection of 22.5 mg/kg methyl methane sulfonate (mms). 0--24 h later [3h]-thymidine was injected in both testicles. incorporation of the isotope in germ cell dna was demonstrated in ejaculated sperms. in controls labeled sperms were demonstrated first on day 40--43. these cells were in the preleptotene spermatocyte phase at the time of [3h]-thmidine injection. in rabbits treated with mms significant radioactivity occurred in sperms collected from day ... | 1978 | 205193 |
| occurrence of anaerobic bacteria in diseases of the dog and cat. | a survey for anaerobic bacteria was conducted in 314 clinical specimens from dogs and cats. a total of 187 anaerobic isolates in pure and mixed culture were isolated from 111 of the specimens that contained anaerobic bacteria. common isolated included actinomyces (9.1%), clostridium perfringens (19.3%), other clostridium spp (11.2%), peptostreptococcus anaerobius (7.5%), bacteroides melaninogenicus (13.4%), other bacteroides spp (17.6%), and fusobacterium necrophorum (5.3%). anaerobic bacteria w ... | 1979 | 224739 |
| survival of anaerobic and aerobic bacteria on cotton swabs in three transport systems. | the capacity of aerobic and anaerobic bacteria to survive on cotton swabs placed into a dry gassed-out co2-filled tube (dgt), dry sterile aerobic tube (dat), and a tube containing a modified stuarts' transport medium (mst), was assessed. pseudomonas aeruginosa increased in numbers by 2 and 3 logs when stored in mst and dat, respectively. the viability of p. aeruginosa, although retarded when compared to msa and dat, was not adversely affected by the co2 environment in the dgt. the mst maintaine ... | 1975 | 240870 |
| [actinomyces in dental plaque]. | | 1977 | 266558 |
| root surface caries in the molar teeth of rice rats. iii. inhibition of root surface caries by fluoride. | | 1977 | 274467 |
| [growth of non-sporing anaerobes in an oxygen-free blood culture system (author's transl)]. | the efficacy of the commercially available vacutainer blood culture system to support the growth of non-sporing anaerobes was compared with two laboratory-prepared blood culture media (supplemented thioglycollate medium and brain heart infusion). the media were inoculated with 10, 100 and 1000 organisms of the species tested, and the number of colony-forming units was determined at intervals of 8-10 hrs. analogous experiments were performed with batches of the media to which 10% vol/vol of human ... | 1977 | 325956 |
| antimicrobial effect of amniotic fluid. | the antimicrobial effect of amniotic fluid (af) obtained during the first (af1) and second (af2) trimesters was compared with the third (af3) against anaerobic bacteria, such as bacteroides fragilis ss. fragilis (6 strains), eubacterium lentum (3 strains), and peptostreptococcus anaerobius (4 strains). escherichia coli (5 strains) served as a positive control. af1 supported the growth of all 4 anaerobes (except b fragilis for 4 hours) for the entire 24-hour period tested. af2 supported the growt ... | 1978 | 355963 |
| serological reactions of the genus peptostreptococcus. | white male new zealand rabbits were immunized with soluble antigen preparations (sp) of the following gram-positive anaerobic cocci: peptostreptococcus anaerobius atcc 27337 and vpi 5737; p. micros vpi 2618; streptococcus morbillorum atcc 27527; p. parvulus vpi 5229; and p. productus atcc 27340. sp were reacted with homologous and heterologous rabbit antisera in immunodiffusion, immunoelectrophoresis, indirect fluorescent-antibody, and tanned-cell passive hemagglutination tests. even though each ... | 1978 | 357444 |
| effects of storage in an anaerobic transport system on bacteria in known polymicrobial mixtures and in clinical specimens. | an anaerobic transport system (ats) which provides for catalytic removal of oxygen was evaluated by using in vitro-prepared polymicrobial mixtures of logphase bacteria and clinical specimens. inoculated swabs were stored at room temperature in (i) aerobic, (ii) anaerobic glove box, and (iii) ats environments, and bacteria were quantitated after 2, 24, 48, and 72 h. bacteria in a three-part mixture of bacteroides fragilis, peptostreptococcus anaerobius, and escherichia coli and in a five-part mix ... | 1978 | 370142 |
| tyrosine degradation in presumptive identification of peptostreptococcus anaerobius. | a new tyrosine medium was developed and evaluated for the differentiation of peptostreptococcus anaerobius from other anaerobic, gram-positive cocci. the strains included 159 originating from clinical specimens and 13 reference strains received from other workers in the field. only one strain of each species was included in the study from multiple cultures from the same patient. this medium is simple to prepare and can be used in a small clinical laboratory. one hundred seventy-two strains of an ... | 1979 | 379031 |
| incidence and pathological features of spontaneous tumors in athymic nude mice. | systematic observation of 1141 nude mice (swiss background [corrected] strain) that received human tumor transplants revealed 24 spontaneous tumors, 18 of lymphoreticular origin and 6 pulmonary adenomas. spontaneous tumors were seen at an average age of 9.1 months, and 22 of the tumors were seen only in that fraction of our group (324 mice) surviving for 5 months or more (22 of 324 x 100 = 6.8%). transplantation of these tumors to other nude mice was successful in three of five cases. mice trans ... | 1979 | 427772 |
| occupational chronic exposure to organic solvents. vii. metabolism of toluene in man. | | 1979 | 429070 |
| bactericidal effect of cysteine exposed to atmospheric oxygen. | peptostreptococcus anaerobius vpi 4330-1 was exposed to atmospheric oxygen in a dilution bland (0.2% gelatin, salts, resazurin) solution. the organisms were rapidly killed when the solution contained cysteine. the organisms were effectively protected by catalase and horseradish peroxidase as well as by the metal ion-chelating agents 8-hydroxyquinoline and 2,2'-bipyridine. superoxide dismutase increased the rate of killing of the organisms, whereas singlet oxygen quenchers and scavengers of hydro ... | 1979 | 453819 |
| isolation and characterisation of bacteria from abscesses in the subcutis of cats. | thirty-six closed abcesses in the subcutis of cats were examined. of 168 bacterial strains isolated, 121(72%) were anaerobes and 47 (28%) were facultative anaerobes. twenty-six abscesses contained mixtures of facultative anaerobes and anaerobes, six contained anaerobes only and four contained facultative anaerobes only. bacteriodes was the genus most commonly isolated (28.6% of all isolates) followed by fusobacterium (19.0%) and pasteurella (multocida) (13.1%). peptostreptococcus anaerobius was ... | 1979 | 458840 |
| description of strains of peptostreptococcus anaerobius isolated from subcutaneous abscesses in cats. | strains of peptostreptococcus, streptococcus and of a gram-positive coccus, which was initially isolated as an anaerobe but grew subsequently as a facultative organism, were isolated from subcutaneous abscesses in cats. the cat strains of peptostreptococcus gave metabolic fermentation products in combinations described for p. anaerobius. the streptococcus strains conformed to the group s. intermedius. the facultative organism described had the metabolic products of p. anaerobius but the distinct ... | 1979 | 479840 |
| obligate anaerobes in clinical veterinary practice. | clinical specimens obtained from domestic animals were examined to determine the relative prevalence of obligate anaerobic bacteria and the species represented. of 3,167 samples cultured anaerobically as well as aerobically, 2,234 were bacteriologically positive. of these positive samples, 583 (26%) contained species of obligate anaerobic bacteria in a total of 641 isolates. most positive samples contained anaerobes admixed with aerobic species, although 6% of such samples yielded pure cultures ... | 1979 | 511987 |
| [on the stiffness of human tibialis anterior muscle during voluntary contraction (author's transl)]. | | 1979 | 522295 |
| differentiation of peptococcus and peptostreptococcus by gas-liquid chromatography of cellular fatty acids and metabolic products. | gas-liquid chromatographic (glc) profiles of cellular fatty acids and metabolic products were useful in identifying strains of peptococcus saccharolyticus, peptococcus asaccharolyticus, peptostreptococcus anaerobius, peptostreptococcus micros, and streptococcus intermedius. the glc results supported the recent taxonomic decision to transfer aerotolerant peptostreptococcus species to the genus streptococcus. because inconsistencies in the results prevented our differentiating peptococcus prevotii ... | 1979 | 528680 |
| [influence of phenobarbital on hyperbilirubinemia caused by nicotinic acid and rifamycin--sv in healthy subjects]. | ten healthy subjects have been given before and after treatment with phenobarbital the following intravenous charges: a) nicotinic acid; b) rifamicyn-sv; c) simultaneous administration of the two drugs. along with confirmatory evidence of the wellknown increase in bilirubinaemia (prevalently unconjugated), we have observed phenobarbital to attempts partially yet significantly that effect. on the base of direct experience on rat liver, we hypothise phenobarbital to increase the pool of the y prot ... | 1979 | 548051 |
| bovine superoxide dismutase and copper ions potentiate the bactericidal effect of autoxidizing cysteine. | when cysteine is oxidized by oxygen, hydrogen peroxide is formed, and hydrogen peroxide is very toxic to peptostreptococcus anaerobius vpi 4330-1. native and inactivated superoxide dismutase increased the rate of oxidation of cysteine and thereby potentiated the toxic effect of cysteine. a similar increase in the rate of oxidation of cysteine and in the toxicity of cysteine was obtained with cu2+. | 1979 | 573589 |
| susceptibility to erythromycin of anaerobes of the genera bacteroides, fusobacterium, sphaerophorus, veillonella, clostridium, corynebacterium, peptococcus, peptostreptococcus. | the minimal inhibitory concentrations (mic) of erythromycin were determined by broth dilution tests for 313 anaerobic strains, most of which were clinical isolates. all the gram-positive anaerobes tested (84 peptococcaceae, including 21 peptostreptococcus anaerobius and 15 peptococcus variabilis; 65 corynebacterium acnes and 29 clostridium strains, including 13 c. perfringens) were sensitive (mic values 0.012 through 3.12 microgram erythromycin/ml); so were 111 cultures of gram-negative anaerobe ... | 1977 | 580038 |
| enhancement of experimental anaerobic infections by blood, hemoglobin, and hemostatic agents. | certain foreign materials have been demonstrated to enhance the infectivity of aerobic and anaerobic bacteria. whole blood and other protein compounds encountered in surgical settings or trauma were tested for their effect on infectivity of nonsporeforming anaerobic bacteria. infectious synergistic mixtures of bacteroides fragilis plus peptostreptococcus anaerobius and bacteroides melaninogenicus plus fusobacterium necrophorum were each diluted to a barely noninfectious or minimally infectious c ... | 1978 | 631883 |
| factors related to the oxygen tolerance of anaerobic bacteria. | the effect of atmospheric oxygen on the viability of 13 strains of anaerobic bacteria, two strains of facultative bacteria, and one aerobic organism was examined. there were great variations in oxygen tolerance among the bacteria. all facultative bacteria survived more than 72 h of exposure to atmospheric oxygen. the survival time for anaerobes ranged from less than 45 min for peptostreptococcus anaerobius to more than 72 h for two clostridium perfringens strains. an effort was made to relate th ... | 1978 | 697363 |
| medium-dependent inhibition of peptostreptococcus anaerobius by sodium polyanetholsulfonate in blood culture media. | of 13 species of anaerobic cocci, peptostreptococcus anaerobius was the only species tested that was sensitive to 0.1% sodium polyanetholsulfonate (sps). however, the sensitivity of p. anaerobius to sps varied according to the media in which the cultures were grown. in supplemented peptone (b-d) and brain heart infusion media, most strain of p. anaerobius were not inhibited by sps. gelatin and proteose peptone were the medium components which were protective. the minimal inhibitory concentration ... | 1976 | 770497 |
| cellular fatty acids of peptococcus variabilis and peptostreptococcus anaerobius. | cellular fatty acids of peptococcus variables and peptostreptococcus anaerobius were identified by gas chromatography, mass spectrometry, and associated analytical techniques. iso- and anteiso-branched-chain acids were major components in both species. | 1977 | 886004 |
| bactericidal effect of anaerobic broth exposed to atmospheric oxygen tested on peptostreptococcus anaerobius. | peptostreptococcus anaerobius strain vpi 4330-1 was used as the test organism in an evaluation of the bactericidal effect of anaerobic broth exposed to air. the test organism, grown under anaerobic conditions in trypticase soy broth, was diluted in buffered salt solution, and about 2 x 10(4) cells were suspended in 10 ml of an aerated broth. ninety percent of the cells were killed within 15 min in actinomyces broth and within 50 min in trypticase soy broth. all cells survived for 2 h in fluid th ... | 1977 | 893657 |
| anaerobic infections in hospital practice. | the role of anaerobes in the pathogenesis of infections was investigated. anaerobes were isolated from 0,25% of blood cultures and from 15,8% of other specimens tested; in 15,1% of cases where anaerobes were isolated, no aerobes were found. the strains most commonly encountered were bacteroides fragilis, b. melaninogenicus, peptostreptococcus anaerobius and clostridium perfringens. sensitivity tests in vitro showed all organisms to be sensitive to clindamycin, metronidazole and chloramphenicol, ... | 1976 | 973160 |
| [brain abscess secondary to pulmonary arteriovenous malformation, case report (author's transl)]. | a case of brain abscess in the right parietal lobe secondary to pulmonary arteriovenous malformation of the right lung is presented. a 27-year-old man was admitted for headache and left homonymous hemianopsia. cag, vag and brain scan detected brain abscess of the right parietal lobe which was successfully removed surgically. he had been noted to have generalized cyanosis and club finger which corresponded to polycytemia and hypercapnia on examination. subsequently pulmonary arteriovenous malform ... | 1976 | 988496 |
| long-chain fatty acids of peptococci and peptostreptococci. | the long-chain fatty acids extracted from the whole cells of 12 clinically significant species of peptococci and peptostreptococci were characterized by gas-liquid chromatography. the resulting methylated fatty acid profiles (and some unidentified compounds) of 82 strains allowed the 12 species to be separated into four groups. fifteen strains of peptostreptococcus anaerobius were placed in group i because they had a unique, prominent compound that occurred in the area where a c8 to c10 fatty ac ... | 1976 | 1002830 |
| comparative susceptibility of anaerobic bacteria to minocycline, doxycycline, and tetracycline. | the comparative susceptibility of 622 recent clinical isolates of anaerobic bacteria to minocycline, doxycycline, and tetracycline was determined by an agar-dilution technique. in addition to bacteroides fragilis, a variety of other anaerobic bacteria was resistant to achievable blood concentrations of tetracycline (55% inhibited by 6.25 mug/ml) and doxycycline (58% inhibited by 2.5 mug/ml). in contrast, minocycline was significantly more active (p < 0.05) than both doxycycline and tetracycline, ... | 1975 | 1137358 |
| [effect of x-rays on human adult skin explants]. | | 1975 | 1233920 |
| [influence of endogenous factors on the ash, calcium, magnesium, potassium, sodium, phosphorus, zinc, iron, copper and manganese contents in the bristles of pigs. 3. influence of time of cutting on the mineral composition of the hair (boar bristles)]. | the ash content and the content of ca, mg, k, and na in the bristles of pigs is influenced by the age of the animal and by the season of the year. the peak concentrations of ash, ca and mg in the bristles of eight boars (cut every month) were found in june, shortly before the change in hair coat. (p less than 0.05-0.001). the concentrations of k and na in the bristles deviated functionally from the change in the hair coat. in the first half of the year the bristles of the boars contained signifi ... | 1975 | 1233935 |
| [treatment and monitoring of primary hypothyroidism]. | treatment of primary hypothyroidism requires a long-term hormonal therapy with levothyroxine which restores a normal tsh value. in a series of 192 patients followed up for at least 3 years we tried to evaluate the variability of maintenance dosage and to analyse the factors predictive of replacement dosage. the l-t4 dosage varied from one individual to the other, from 0.6 to 3.2 micrograms/kg. the mean maintenance dosage (118 +/- 38 micrograms/day) was similar in men and women weight for weight, ... | 1992 | 1287777 |
| antibacterial efficacy of root canal medicaments. | the antibacterial efficacy of four endodontic medicaments was studied in vitro. calcium hydroxide (calacept), camphorated parachlorophenol (cpcp), cresophene, and 2% iodine-potassium iodide (iki) were tested against four anaerobic microorganisms, streptococcus mutans, peptostreptococcus anaerobius, porphyromonus gingivalis and fusobacterium nucleatum, for 10 or 15 min. iki was effective only against f. nucleatum and p. gingivalis, and the others were effective against all four microorganisms. | 1992 | 1337555 |
| detubularization-induced contractile response change of the ileum following ileocystoplasty. | we reported previously that following ileocystoplasty the structure and pharmacologic response of the implanted ileum changes towards that of the bladder. specifically, the relaxation response to alpha adrenergic (methoxamine) and purinergic (atp) stimulation reverses to a contractile response one month after the ileal segment is surgically made part of the urinary bladder. the present study was designed to investigate possible signals for this change and also to determine whether bladder respon ... | 1992 | 1351953 |
| periodontal bone loss in mice induced by different periodontopathic organisms. | periodontal bone loss in mice orally inoculated with peptostreptococcus anaerobius, pept. magnus and actinobacillus actinomycetemcomitans was compared to that in sham-inoculated mice. six-to-8-week-old balb/c mice were inoculated with 1 x 10(5), 1 x 10(7) or 1 x 10(9) colony-forming units (c.f.u.) of bacteria in 50 microliters of medium. ten mice received each concentration of bacteria and 10 sham-inoculated mice acted as controls. five mice from each of the groups were killed 6 weeks after inoc ... | 1992 | 1353347 |
| experimentally induced infection by oral anaerobic microorganisms in a mouse model. | a mouse model was developed to study the abscessogenic potential of pure and mixed cultures of oral anaerobes associated with infections of endodontic origin. maximum subcutaneous lesions were seen on their backs between 3 and 14 d after inoculation. strains of fusobacterium nucleatum, peptostreptococcus anaerobius, and veillonella parvula were pathogenic in pure culture. a mixed culture of f. nucleatum with either porphyromonas gingivalis or prevotella intermedia was significantly more pathogen ... | 1992 | 1408362 |
| experimental studies of survival of anaerobic bacteria at 4 degrees c and 22 degrees c in two different transport systems. | the survival of anaerobic bacteria on swabs in two different transport systems at 4 degrees c and 22 degrees c was evaluated. the transport systems were a charcoal-impregnated cotton swab in modified stuart transport medium (mst), and a viscose swab in modified siff transport medium (btm) (bionor). the following eight clinical strains of anaerobic bacteria were tested for quantitative recovery at 24 h, 48 h, 72 h, and 96 h; fusobacterium necrophorum, bacteroides melaninogenicus, bacteroides inte ... | 1992 | 1472363 |
| the relative lethality of intestinal bacteria for gnotobiotic rats with experimental intestinal strangulation. | in eight experiments utilizing 28 animals each, germfree rats with and without ischemically or hemorrhagically strangulated closed loop intestinal segments were contaminated with either one of four common intestinal anaerobic bacteria, or a combination of two or three bacteria previously found to be innocuous in pure culture. the results showed that: a) in pure culture, bacteroides fragilis, bacteroides melaninogenicus, and peptostreptococcus anaerobius were innocuous; b) fusobacterium necrophor ... | 1992 | 1479304 |
| associations between microbial species in dental root canal infections. | the existence of commensal or antagonistic relationships between microorganisms in the root canals of teeth with apical periodontitis was investigated. samples were taken from 65 infected human root canals and were analysed according to species, frequency of occurrence and proportion of the total isolated flora. the most frequent species were fusobacterium nucleatum, prevotella intermedia, peptostreptococcus micros, peptostreptococcus anaerobius, eubacterium alactolyticum, eubacterium lentum and ... | 1992 | 1494447 |
| 2,6-dichlorophenolindophenol is a competitive inhibitor for xanthine oxidase and is therefore not usable as an electron acceptor in the fluorometric assay. | xanthine oxidase has been recognized as an important source of oxygen free radicals in ischemia-reperfusion injury. in order to study this enzyme in biological tissues, the conversion of pterin (2-amino-4-hydroxypteridine) to isoxanthopterin provides the basis for a very sensitive fluorometric assay. xanthine oxidase is typically assayed in the presence of pterin only, while an electron acceptor which replaces nad+ is used to determine the combined xanthine dehydrogenase plus xanthine oxidase ac ... | 1992 | 1563644 |
| thermogenic and hormonal responses to palatable protein and carbohydrate rich food. | simultaneous variations of oxygen consumption, and plasma insulin and norepinephrine were measured during the postprandial cephalic and gastrointestinal phases of feeding in six human subjects following the ingestion of various nutrients. on alternative days the subjects were given foods (1280 kjoules) either rich in carbohydrates (sugar pie) or in proteins (fish). both nutrients produced an initial (0-40 min) enhanced thermogenesis and an early (2 min) cephalic insulin release. during that peri ... | 1991 | 1774019 |
| comparative in vitro activity of cefpodoxime against anaerobes other than bacteroides fragilis. | to assess the in vitro activity of cefpodoxime against anaerobic respiratory tract and oropharyngeal pathogens 77 strains belonging to 18 gram-negative and 7 gram-positive species were studied by means of agar dilution tests. for comparison cefuroxime, amoxicillin, amoxicillin + clavulanic acid and clindamycin were also tested. cefpodoxime was found to be active at concentrations of less than or equal to 0.125 mg/l against prevotella oralis, prevotella buccalis, prevotella bivia, porphyromonas a ... | 1991 | 1800380 |
| normal vaginal aerobic and anaerobic bacterial flora of the rhesus macaque (macaca mulatta). | the most common bacterial species isolated from the vaginas of 37 healthy rhesus macaques were streptococcus viridans, coagulase negative staphylococcus, mobiluncus curtisii ss. curtisii, corynebacterium renale-like organisms, peptostreptococcus anaerobius, gardnerella-like organisms, and other corynebacterium species. the vaginal flora of the rhesus macaque differs from that previously reported for five other primate species. a two-year retrospective review of clinical cases of vaginitis and me ... | 1991 | 1803013 |
| [empyema due to an anaerobic streptococcus in childhood]. | anaerobic infections are quite rare in pediatric age, being that, they affect only neonates and immunodepressed patients. we think to be somewhat interesting to describe the case of our patient, a 9 year old boy, unaffected by any predisposing factor, came under our observation because of a severe respiratory distress. he showed evident clinical and radiological signs of pleural effusion in the right lung, together with a gas coil in the upper field and a left mediastinal shifting. a thoracentes ... | 1991 | 1806927 |
| formation of nitrogen-containing metabolites from geniposide and gardenoside by human intestinal bacteria. | during the course of our studies on the metabolism of iridoid glycosides by human intestinal bacteria, we found that geniposide (1) and gardenoside (4) were transformed to new nitrogen-containing compounds, genipinine (3) and gardenine (6), respectively, along with the known aglycones. although the amounts of new metabolites were somewhat lower than those of the aglycones, they were quantitatively analyzed by means of liquid chromatography/mass spectrometry (lc/ms). of 25 strains of human intest ... | 1991 | 1818345 |
| bidirectional morphological changes induced by dexamethasone in morris hepatoma cell lines mca-rh7777 and mca-rh8994: independence of fibronectin and its receptor. | two morris hepatoma-derived cell lines, mca-rh7777 (7777) and mca-rh8994 (8994), exhibit different alterations in morphology upon exposure to glucocorticoid. after treatment with synthetic glucocorticoid dexamethasone (dex), 7777 cells show increased adhesiveness and more flattened shape, while dex-treated 8994 cells show decreased adhesiveness to substratum and exhibit a marked increase of round and detached cells. since fibronectin has been thought to play an important role in cell adhesivenes ... | 1991 | 1829041 |
| human and health rights of palestinians in the occupied territories. essay review. | | 1991 | 1865855 |
| association of antisense oligonucleotides with lipoproteins prolongs the plasma half-life and modifies the tissue distribution. | in order to direct antisense oligonucleotides to specific tissues or cell types in vivo, we are exploring the possibility to utilize lipoproteins as transport vehicles. a 16-mer oligonucleotide (odn) was derivatized at the 5' prime through a 32p phosphate spacer with cholesterol, yielding a 32p-labeled amphiphatic cholesteryl-oligonucleotide (cholodn). incubation of cholodn with low-density lipoprotein (ldl) for 2 hr at 37 degrees c resulted in the formation of a cholodn-ldl complex that migrate ... | 1991 | 1891360 |
| protein-kinase-c-independent activation of arachidonate release and prostaglandin e2 formation in macrophages interacting with certain bacteria. | certain bacterial species, of which we selected fusobacterium nucleatum, gardnerella vaginalis, peptostreptococcus anaerobius and propionibacterium acnes, were found to induce release of arachidonic acid in a dose- and time-dependent manner in mouse macrophages. the release of arachidonic acid showed a characteristic lag period of approximately 10 min and was accompanied by selective transformation into prostaglandin e2. bacteria killed by various methods caused a similar response, indicating th ... | 1991 | 1915341 |
| tetracycline resistance in peptostreptococcus species. | of 15 peptostreptococcus sp. strains isolated between 1975 and 1984, 13 hybridized with the tet k, tet m, or tet o determinant. a donor peptostreptococcus anaerobius strain carrying the three determinants could transfer tet m to p. anaerobius and fusobacterium nucleatum recipients but not to an enterococcus faecalis recipient, while neither tet k nor tet o was transferred. | 1991 | 1929341 |
| new strategies in the development of thrombolytic agents. | | 1991 | 1954777 |
| comparative efficacy of cefoperazone, cefoperazone plus sulbactam, ciprofloxacin, clindamycin, metronidazole, and penicillin g against anaerobic bacteria in an animal model. | treatment efficacy of various antimicrobial regimens against anaerobes was studied in semipermeable chambers simulating a closed-space, locally neutropenic infection site in rabbits. bacteroides fragilis, bacteroides melaninogenicus, clostridium perfringens, and peptostreptococcus anaerobius were inoculated (at a mean of 5.3 log10 cfu/ml in prereduced pooled rabbit serum) into the chambers (one isolate per chamber) in triplicate. antimicrobial therapy consisted of cefoperazone, cefoperazone plus ... | 1990 | 1967626 |
| [anaerobic bacteria and their role in recurrent tonsillitis]. | the presence of anaerobic bacteria in 100 palatal tonsils obtained during the tonsillectomy, was evaluated in quality and in quantity. 17 species of anaerobic bacteria were found. the most frequent were: bacteroides melaninogenicus, peptostreptococcus anaerobius, peptostreptococcus intermedius, propionibacterium, veillonella parvula and peptococcus asaccharolyticus. in most evaluations their density was 10(5)-10(6) in gram of tonsil tissue, sometimes 10(7). its role in recurrent tonsils infectio ... | 1990 | 1981606 |
| the differentiation of asaccharolytic anaerobic gram-positive cocci by protein electrophoresis. | conventional biochemical and antibiotic sensitivity tests were used to allocate 87 clinical isolates of anaerobic gram-positive cocci to currently recognised species, in comparison with type and other authentic reference strains. whole-cell protein electrophoresis was then performed with extracts of each strain. allowing for difficulties of standardisation, it was possible to allocate most of the organisms to species-related groups on the basis of protein patterns. organisms identified conventio ... | 1991 | 2056518 |
| polysaccharides from peptostreptococcus anaerobius and structure of the species-specific antigen. | the cell-envelope antigens of peptostreptococcus anaerobius were extracted from intact cells by autoclave or alkaline treatment. the purified species-specific antigen (g) was identified among several polysaccharides obtained from the extracts by successive treatments with ribonuclease and pronase followed by ion-exchange and gel-filtration chromatography. g was investigated by 13c- and 31p-n.m.r. spectroscopy, titrimetry, elemental analysis, and gas-liquid chromatography. oxidation of g with nai ... | 1990 | 2076510 |
| bactericidal effects of human neutrophils and sera on selected endodontic pathogenic bacteria in an anaerobic environment. | phagocytosis by normal human neutrophils and the bactericidal activity of normal human pooled serum were measured under anaerobic conditions on six endodontic pathogenic bacterial species from the genera actinomyces, bacteroides, fusobacterium, peptostreptococcus and streptococcus. the results revealed that actinomyces viscosus was not killed either by human neutrophils or by 20 per cent serum; peptostreptococcus anaerobius was not killed by either 20 per cent or 95 per cent serum; and bacteroid ... | 1990 | 2098335 |
| a rapid diagnosis of anaerobic infection in the oro-maxillary region by gas-liquid chromatography. | the relationship between volatile fatty acids (vfas) in pus and infecting bacterial species was examined in order to establish a rapid identification system for anaerobic microorganisms in purulent inflammation in the oro-maxillary region. vfas were detected by the direct injection of pus into gas-liquid chromatography (glc). bacterial examination was carried out by anaerobic culture using blood agar plates. the bacterial identification was carried out mainly according to the vpi manual. analysi ... | 1990 | 2131168 |
| demonstration of the heterogeneity of the kappa-opioid receptors in guinea-pig cerebellum using selective and nonselective drugs. | in guinea-pig cerebellum, saturation studies reveal that the nonselective opioid [3h]ethylketazocine has a binding capacity (r) of 6.79 pmol/g tissue which is similar to the sum of the individual r values of the mu-, delta- and kappa 1-selective opioids. conversely, the binding parameters of the nonselective opioid [3h]bremazocine are best-fitted to a two-site model (kd1 = 0.12 nm, r1 = 11.3 pmol/g tissue; kd2 = 6.03 nm, r2 = 9.09 pmol/g tissue) with an r total value of 20.3 pmol/g tissue which ... | 1990 | 2164939 |
| [new antitubercular drugs]. | | 1990 | 2168041 |
| transferable tet m in fusobacterium nucleatum. | two tetracycline-resistant fusobacterium nucleatum strains were able to transfer, by conjugation, tetracycline resistance and the tet m determinant to recipient f. nucleatum, peptostreptococcus anaerobius, and enterococcus faecalis strains at a frequency of 10(-1) to 10(-8). | 1990 | 2178339 |
| formation of amphetamine from its nitro analogue by anaerobic intestinal bacteria. | 1. microbial reduction of 1-phenyl-2-nitropropane 1 was carried out using 40 strains of intestinal anaerobic bacteria. among them, 12 strains (mitsuokella multiacidus, clostridium perfringens, c. innocuum, c. clostiriiforme, c. difficile, c. butyricum, c. sp., eubacterium limosum, e. aerofaciens, e. multiforme, peptostreptococcus anaerobius and p. productus) had the ability to reduce 1 to amphetamine 2 (0.1-1% yield). 2. clostridium species were more active than another intestinal anaerobic bact ... | 1990 | 2219956 |
| use of modified norleucine-tyrosine broth in identification of peptostreptococcus anaerobius. | gas-liquid chromatography was employed to analyze the volatile and nonvolatile acids produced in modified norleucine-tyrosine (mnt) broth by various gram-positive cocci. the mnt broth consists of 0.5% trypticase (bbl microbiology systems, cockeysville, md.), 0.5% yeast extract (difco laboratories, detroit, mich.), 0.2% l-norleucine, and 0.1% l-tyrosine. the microorganisms included reference strains and clinical isolates of peptostreptococcus spp. (p. anaerobius, p. asaccharolyticus, p. indolicus ... | 1990 | 2229394 |
| [a clinical study on ceftazidime in the treatment of intractable respiratory infections]. | ceftazidime (caz) was administered to 51 patients (37 males, 14 females) with respiratory infections including severe cases, accompanied by various underlying respiratory diseases. the clinical efficacy and side effects of caz were investigated. the mean age and body weight of these 51 cases were 62.6 years and 48.0 kg, respectively. caz was administered by intravenous drip infusion (daily dose of 2-4 g) for a mean of 14.7 days to a mean total dose of 56.7 g. clinical efficacy rates were 64% (18 ... | 1990 | 2232147 |
| isolation and identification of anaerobic bacteria from ovine foot rot in spain. | a microbiological study was made of 125 merino sheep showing clinical signs of foot rot. a total of 435 strictly anaerobic strains were isolated, belonging to the following genera: bacteroides, peptostreptococcus, tissierella, fusobacterium, megasphaera, eubacterium, acidaminococcus, clostridium, peptococcus and propionibacterium. of the 35 species obtained, the following were found in more than 10 per cent of animals sampled: bacteroides nodosus, b putredinis, b buccae, b ruminicola subspecies ... | 1990 | 2236925 |
| obligately anaerobic bacterial species isolated from foot-rot lesions in goats. | lesions showing clinical signs of foot-rot from 120 goats were cultured on six selective media during october 1987 to november 1988. a total of 582 strictly anaerobic microorganisms belonging to 50 different species was isolated and identified. the anaerobes most frequently isolated belonged to the following genera: bacteroides (80%), peptostreptococcus (63.6%), megasphaera (40%), fusobacterium (29.2%), clostridium (22.5%), propionibacterium (12.5%), eubacterium (11.7%) and leptotrichia (10.8%). ... | 1990 | 2271912 |
| the obligate and facultatively anaerobic bacterial flora of the normal feline gingival margin. | samples from the gingival margins of 14 cats considered normal on clinical examination were cultured for facultative and obligate anaerobic bacteria. all mouths were free from any gingival marginal inflammation and tartar build-up; all cats were between 6 and 12 months of age. a mixed growth was obtained from all samples. the mean number of bacterial species per sample was 10.7 with a range of 7-16 isolates. of the 150 isolates processed, 109 (72.66%) were obligate anaerobes. of the facultativel ... | 1990 | 2353448 |