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differentiation of several isolates of peptococcus magnus by counterimmunoelectrophoresis.uniform sonicated suspensions of three clinical isolates of peptococcus magnus that were morphologically, chromatographically, and biochemically identical were used to produce hyperimmune antisera in rabbits. similar hyperimmune serum was prepared to a single strain of peptostreptococcus anaerobius. when these antisera and antigens were tested by counterimmunoelectrophoresis, the three isolates of peptococcus magnus were immunologically distinct. none of the antisera to peptococcus magnus formed ...197768929
dietary fibre and experimental colon cancer. 197985018
acid fast staining of oxidized neuromelanin and lipofuscin in the human brain.acid fast staining of the bleached residuum of substantia nigra neuromelanin and of oxidized inferior olive lipofuscin was demonstrated in paraffin and frozen sections stained with the acetic acid, carbol fuchsin method of barbeito-lopez and the aldehyde fuchsin method of gomori. acid fast staining occurred when sections were exposed to a prestain oxidation with potassium permanganate in conjunction with a poststain differentiation with dilute hydrochloric acid. the acid fast staining with aceti ...197989189
distribution of myosin isoenzymes among skeletal muscle fiber types.using an immunocytochemical approach, we have demonstrated a preferential distribution of myosin isoenzymes with respect to the pattern of fiber types in skeletal muscles of the rat. in an earlier study, we had shown that fluorescein-labeled antibody against "white" myosin from the chicken pectoralis stained all the white, intermediate and about half the red fibers of the rat diaphragm, a fast-twitch muscle (gauthier and lowey, 1977). we have now extended this study to include antibodies prepare ...197990047
[intratypical antigenic differentiation of poliovirus strains circulating in mali by means of cross-adsorbed sera].the intratypic antigenic differentiation of poliovirus strains isolated from sick or healthy children in mali in 1975-1976 was performed. the method is based on the use of cross-adsorbed sera in the neutralization tests in which the virus is titrated by the plaque procedure or by the cytopathic effect. all the examined strains of serologic types i, ii, and iii were distinguished by the antigenic structure from the vaccine strains and were classified as "wild". quantitative differences in the ant ...197886237
relationship of intracellular 3',5'-adenosine monophosphate accumulation to parathyroid hormone release from dispersed bovine parathyroid cells. 197885548
the allergenic structure of allergen m from cod. iii. studies on the antigenicity of long-sequence peptides.fragments tm 1 (75 amino acid residues) and tm 2 (38 amino acid residues), and 3 other polypeptides (range 16-25 amino acid residues) of allergen m from cod were shown to be active in rabbit igg-mediated reactions. the same peptides were previously found to possess reactivity in ige-mediated reactions, thus suggesting a structural relationship between their antigenic and allergenic determinants.197984803
polyribonucleotide inhibition of ribonucleic acid directed deoxyribonucleic acid polymerase of mouse mammary tumor (type b) virus and simian sarcoma (type c) virus. 197990331
the elephant man. 197990342
new antibiotics, enaminomycins a, b and c. ii. physico-chemical and biological properties.physico-chemical characterization of enaminomycins revealed that these antibiotics are new members of the epoxy quinone family. from elementary analysis and mass spectroscopic measurements the molecular formulae of enaminomycins a, b and c appear to be c7h5no5, c10h11n06 and c7h7no5, respectively. they are very unique in their chemical properties, possessing various functions, such as epoxy, primary amine and carboxylic acid, in their small structural units. enaminomycin a, the most potent compo ...1978101500
[animal experimental studies on the volume expanding effect of hydroxyethyl starch 40000 in the dog acute hemorrhagic shock].after acute hemorrhagic shock in dogs the hemodynamics of hydroxyethyl starch (mw: 40,000) are studied. the experiment shows that this plasma expander has a good efficiency for 3 to 4 hours. the indications of application of this colloidal solution are discussed.197990651
the indirect rat mast cell degranulation test reconsidered.the indirect rat mast cell degranulation test for the detection of human reaginic antibodies was reinvestigated. when the mast cells were incubated with human reaginic serum at concentrations exceeding approximately 3%, a massive, non-specific histamine release occurred, irrespective of the presence or absence of allergen. at tolerated serum concentrations (below 3%), the allergen concentration did not influence the release process. we conclude that the indirect rat mast cell test is not applica ...197990655
effect of ethylenediaminetetraacetate on phospholipids and outer membrane function in escherichia coli.treatment of escherichia coli k-12 strain s15, containing a normal amount of phospholipase a, with ethylenediaminetetraacetate (edta) resulted in an increase in sensitivity of the organism to actinomycin d. strain s17, a mutant deficient in both detergent-resistant phospholipase a and detergent-sensitive phospholipase a, was considerably less sensitive to the antibiotic after the treatment. both strains released lipopolysaccharide after edta treatment, indicating that this outer membrane compone ...1979104974
[effect of polychlorinated biphenyls in the rat: ii.--preliminary report on metabolic alterations (author's transl)].rats were fed with a diet containing 10 and 100 ppm (wet weight) of phenoclor dpr for 4 weeks in order to evaluate the toxicity of a french pcb mixture. the influence of pcb was studied by determining the liver weights, the hepatic and muscular proteins, glycogen and humidity content as well as hematocrite, glucose, proteins and lipids in the plasma, and the lipidic contents of the liver and the carcass. there was a significant increase in both liver to body weight ratios and liver lipid and pro ...1978105425
multiple sclerosis: immunochemical studies on the demyelinating serum factor.sera from patients with multiple sclerosis (ms) frequently produce demyelination of central nervous system tissue cultures. the nature of the factors responsible for demyelination is not as yet clearly established. however, several authors previously reported, in in vivo and in vitro models, demyelinating activity in igg fractions isolated from sera and cerebrospinal fluid of ms patients14,42,48. we found the demyelinating activity of ms sera to be extremely labile to conventional biochemical tr ...1979105780
radiation carcinogenesis.accidental or therapeutic exposure to radiation may induce tumors of various histologic types in human beings as well as in animals. two tumors in this report arose in the organs which had been included in the field of radiation 32 and 8 years before, respectively. the author briefly reviews the literature, which abounds with references to radiation carcinogenesis.1975126797
differential effects of oxygen and oxidation-reduction potential on the multiplication of three species of anaerobic intestinal bacteria.the sensitivity of three strains of anaerobic intestinal bacteria, clostridium perfringens, bacteroides fragilis, and peptococcus magnus, to the differential effects of oxygen and adverse oxidation-reduction potential was measured. the multiplication of the three organisms was inhibited in the presence of oxygen whether the medium was at a negative oxidation-reduction potential (eh of -50 mv), poised by the intermittent addition of dithiothreitol, or at a positive oxidation-reduction potential ( ...1975173238
calcium uptake and atpase activity of microsomes from heterotopically transplanted rabbit heart.heterotopic cardiac transplantation was performed in rabbits. the calcium uptake and atpase activities of microsomal fraction were studied in three groups (group i, normal controls; group ii, early transplants; group iii, late transplants) during severe rejection as characterized histologically and electrocardiographically. group iii showed a significant decrease in calcium uptake but no difference in atpase activity compared with groups i or ii. the results demonstrate that in the rejecting tra ...1975127353
nucleoside diphosphokinase of rat heart mitochondria. dual localization in matrix and intermembrane space. 1977193861
histochemistry and histoenzymology of the hydatid cyst (echinococcus granulosus batsch, 1786). ii. scolices and brood capsules.scolices and brood capsules of healthy hydatid cysts from lungs of human patients were studied with histochemical and histoenzymatic methods. the subtegumental and flame cells were sepcially rich in glycogen, rna and some dehydrogenases such as sdh, mdh, nadh-reductase and g-6-pdh. the rostellar zone or invaginated pole, an area of marked contractile movements, showed intense activity in atp'ase and simple esterase. the so-called excretory pole shows strong activity in simple esterases, lipase, ...1975130750
a nuclear mutation conferring aurovertin resistance to yeast mitochondrial adenosine triphosphatase.a single gene nuclear yeast mutant was isolated whose mitochondrial f1-atpase was resistant to the specific f1 inhibitor aurovertin. the mutant enzyme was not cross-resistant to other f1 inhibitors. the binding of aurovertin to f1 and to the two largest f1 subunits (alpha and beta) was measured by enhancement of aurovertin fluorescence. aurovertin bound to wild type f1-atpase and to its monomeric beta subunit with about the same binding constant. it failed to bind to wild type alpha subunit or t ...1979153911
[influence of horizontal immersion on urinary excretion of catecholamines].normal man submitted to thermoneutral water immersion in horizontal position presents an increase in diuresis, natriuresis and creatininuria. noradrenalinuria and adrenalinuria are reduced, indicating a decrease in orthosympathetic activity.1977148963
infection of the foot with peptococcus magnus.peptococcus magnus was the predominant organism in severe infections of the feet in three diabetic patients. in one patient the organism was found also in blood culture, and in two it was repeatedly recovered from the feet, usually in pure culture.1977845274
biological characteristics of a stimulatory factor for viral replication detected in egg fluids.yolk sac and allantoic fluids and albumen from uninfected chicken eggs contain a low-molecular weight factor which, after 90 minutes of contact with cell cultures, significantly enhances viral replication. of several viruses tested, semliki forest virus exhibited the highest (10(5)-fold) responsiveness to this enhancing factor. maximal enhancement was obtained with cells subjected to low multiplicities of infection. the stimulation of viral replication was linear with the age of the cells in cul ...1978415702
effectiveness of orally administered bordetella pertussis vaccine in mice.oral administration of killed bordetella pertussis organisms to mice results in increased resistance to an intracerebral infection with virulent b. pertussis cells. the rate of survival is dependent on the dose of antigen. but besides specific systemic immunity, which is persistent over a long period, also transient non-specific resistance is increased. these effects are evidently induced without penetration of bacterial substances into the circulation.1976182602
[actinomyces in dental plaque]. 1977266558
infection of total hip prostheses by peptococcus magnus: an immunofluorescence and elisa study of two cases.in two cases of infected total hip replacements, peptococcus magnus was isolated in pure culture from the implant when it was removed. fluorescent antibody and elisa studies have shown that both patients developed an antibody response to this anaerobic coccus soon after the replacement operation. these results suggest that the organism is a true infective agent, which was probably responsible for the failure of the arthroplasty operation.1979372250
[influence of endogenous factors on the ash, calcium, magnesium, potassium, sodium, phosphorus, zinc, iron, copper and manganese contents in the bristles of pigs. 3. influence of time of cutting on the mineral composition of the hair (boar bristles)].the ash content and the content of ca, mg, k, and na in the bristles of pigs is influenced by the age of the animal and by the season of the year. the peak concentrations of ash, ca and mg in the bristles of eight boars (cut every month) were found in june, shortly before the change in hair coat. (p less than 0.05-0.001). the concentrations of k and na in the bristles deviated functionally from the change in the hair coat. in the first half of the year the bristles of the boars contained signifi ...19751233935
[studies on the protein and amino acid metabolism of laying hens using 15n labeled casein. 15n concentration in the intestinal content and intestinal tissue in relation to the time of the last 15n administration].four laying hens with anus praeternaturalis were fed 15n casein for a period of 6 experimental days. each experimental bird thus received a 15n excess (15n') of 154.44 mg. 11.54 mg of 15n' were found in lysine 3.99 mg in histidine and 9.58 mg in arginine. 2 hens were slaughtered 48 hours after the last 15n dose; another 2 were sacrificed after 144 hrs. in this investigation the n content of the intestinal contents and in the proteins of the small and large intestine were determined. the average ...19751233975
transforming growth factor beta induces myoblast differentiation in the presence of mitogens.transforming growth factor beta 1 (tgf-beta 1) added to l6e9 rat skeletal myoblasts in mitogen-rich medium induces a rapid decrease in c-myc expression and delays progression through the g1 phase of the cell cycle. this growth inhibitory response is followed by cell commitment to terminal differentiation with elevated expression of myogenin muscle determination genes, induction of muscle-specific proteins, and formation of multinucleated myotubes. these results suggest that tgf-beta 1 may act as ...19921594628
long-chain fatty acids of peptococci and peptostreptococci.the long-chain fatty acids extracted from the whole cells of 12 clinically significant species of peptococci and peptostreptococci were characterized by gas-liquid chromatography. the resulting methylated fatty acid profiles (and some unidentified compounds) of 82 strains allowed the 12 species to be separated into four groups. fifteen strains of peptostreptococcus anaerobius were placed in group i because they had a unique, prominent compound that occurred in the area where a c8 to c10 fatty ac ...19761002830
protein lg: a hybrid molecule with unique immunoglobulin binding properties.immunoglobulin (ig)-binding bacterial proteins have attracted theoretical interest for their role in molecular host-parasite interactions, and they are widely used as tools in immunology, biochemistry, medicine, and biotechnology. protein l of the anaerobic bacterial species peptostreptococcus magnus binds ig light chains, whereas streptococcal protein g has affinity for the constant (fc) region of igg. in this report, ig binding parts of protein l and protein g were combined to form a hybrid mo ...19921460053
protein l. a bacterial ig-binding protein that activates human basophils and mast cells.peptostreptococcus magnus strain 312 (10(6) to 10(8)/ml), which synthesizes a protein capable of binding to kappa l chains of human ig (protein l), stimulated the release of histamine from human basophils in vitro. p. magnus strain 644, which does not synthesize protein l, did not induce histamine secretion. soluble protein l (3 x 10(-2) to 3 micrograms/ml) induced histamine release from human basophils. the characteristics of the release reaction were similar to those of rabbit igg anti-fc frag ...19901698870
protein l from peptostreptococcus magnus binds to the kappa light chain variable domain.protein l is an immunoglobulin light chain-binding protein expressed by some strains of the anaerobic bacterial species peptostreptococcus magnus. the major variable region subgroups of human kappa and lambda light chains were tested for protein l binding; v kappa i, v kappa iii, and v kappa iv bound protein l, whereas no binding occurred with proteins of the v kappa ii subgroup or with any lambda light chain subgroups. studies of the protein l binding capacity of naturally occurring vl fragment ...19921733930
structure of peptostreptococcal protein l and identification of a repeated immunoglobulin light chain-binding domain.the gene for protein l, an immunoglobulin (ig) light chain-binding protein expressed by some strains of the anaerobic bacterial species peptostreptococcus magnus, was cloned and sequenced. the gene translates into a protein of 719 amino acid residues. following a signal sequence of 18 amino acids and a nh2-terminal region ("a") of 79 residues, the molecule contains five homologous "b" repeats of 72-76 amino acids each. further, toward the cooh terminus, two additional repeats ("c") were found. t ...19921618782
enzymatically active peptostreptococcus magnus: association with site of infection.fifty-four strains of peptostreptococcus magnus (11 were recovered from abdominal infections, 18 were from nonpuerperal breast abscesses, and 21 were from diabetic foot infections; the type strain and three other strains were from the american type culture collection, rockville, md.) and the type strain of peptostreptococcus micros were tested for their ability to produce various enzymes, including catalase, hippurate hydrolase, serine dehydratase, threonine dehydratase, collagenase, gelatinase, ...19921400997
differentiation of peptococcus and peptostreptococcus by gas-liquid chromatography of cellular fatty acids and metabolic products.gas-liquid chromatographic (glc) profiles of cellular fatty acids and metabolic products were useful in identifying strains of peptococcus saccharolyticus, peptococcus asaccharolyticus, peptostreptococcus anaerobius, peptostreptococcus micros, and streptococcus intermedius. the glc results supported the recent taxonomic decision to transfer aerotolerant peptostreptococcus species to the genus streptococcus. because inconsistencies in the results prevented our differentiating peptococcus prevotii ...1979528680
prosthetic valve endocarditis and paravalvular abscess caused by peptostreptococcus magnus. 19921617069
comparative in vitro activity of cefpodoxime against anaerobes other than bacteroides fragilis.to assess the in vitro activity of cefpodoxime against anaerobic respiratory tract and oropharyngeal pathogens 77 strains belonging to 18 gram-negative and 7 gram-positive species were studied by means of agar dilution tests. for comparison cefuroxime, amoxicillin, amoxicillin + clavulanic acid and clindamycin were also tested. cefpodoxime was found to be active at concentrations of less than or equal to 0.125 mg/l against prevotella oralis, prevotella buccalis, prevotella bivia, porphyromonas a ...19911800380
[microionophoretic study of the mechanism of action of gamma-hydroxybutyrate].the effect of gamma-hydroxybutyric acid (ghba) on the extracellularly registered spontaneous electrical activity of nervous cells of the rabbit brain sensomotor cortex was studied using the microionophoretic technique. ghba decreased the frequency of action potential in the majority of the neurons studied. a specific gamma-aminobutyric acid (gaba) blocking agent bicuculline prevented gbha inhibitory effect. ghba is suggested to interact with central gaba-receptors. the frequency of discharges in ...1979540145
severe anaerobic necrotizing pneumonia complicated by pyopneumothorax and anaerobic monoarthritis due to peptostreptococcus magnus.a rare case of a severe anaerobic necrotizing pneumonia complicated by pyopneumothorax and monoarthritis due to anaerobic peptostreptococcus magnus is described in a 20-year-old soldier. the patient's immunological status was normal. there were no predisposing underlying factors, and he was treated successfully without any residual damage. to our knowledge, a similar case has never been reported in the literature.19911745861
anaerobic pathogenesis: collagenase production by peptostreptococcus magnus and its relationship to site of infection.fifty isolates of peptostreptococcus magnus from intraabdominal sepsis, nonpuerperal breast abscess, and diabetic foot infections were examined for collagenase activity using bovine type i collagen. collagenase production was detected in a higher percentage of strains from nonpuerperal breast and diabetic foot specimens (p less than .001). this enzyme may be responsible for p. magnus playing a more central role in the pathogenesis of nonpuerperal breast abscess and diabetic foot disease than in ...19911850443
experimental studies of survival of anaerobic bacteria at 4 degrees c and 22 degrees c in two different transport systems.the survival of anaerobic bacteria on swabs in two different transport systems at 4 degrees c and 22 degrees c was evaluated. the transport systems were a charcoal-impregnated cotton swab in modified stuart transport medium (mst), and a viscose swab in modified siff transport medium (btm) (bionor). the following eight clinical strains of anaerobic bacteria were tested for quantitative recovery at 24 h, 48 h, 72 h, and 96 h; fusobacterium necrophorum, bacteroides melaninogenicus, bacteroides inte ...19921472363
[study on prototype cylindrical hap].hydroxyapatite (hap) is currently utilized as biomaterials for implantation or reconstruction of bone defect in dentistry. in order to obtain better physical properties and biocompatibility, we have developed cylindrical sintered porous hap which has longitudinal internal tubules. we measured compressive strength and specific surface area of cylindrical hap. in the results, compressive strength was more 300mpa on average and specific surface area was 1.7-2.4 folds as hap ceramics commercially av ...19902134111
endometrial histopathology in patients with culture-proved upper genital tract infection and laparoscopically diagnosed acute salpingitis.to define and quantitate histologic changes in the endometrium that best correlate with documented upper genital tract infection (ugti) and laparoscopically diagnosed acute salpingitis, we studied endometrial biopsy specimens from 69 consecutive patients with clinically suspected acute pelvic inflammatory disease (pid) who underwent microbiological evaluation for ugti and laparoscopic examination for acute salpingitis. both ugti and acute laparoscopically confirmed salpingitis were present in 37 ...19902137304
an analysis of abnormalities of the retinoblastoma gene in human ovarian and endometrial carcinoma.the altered expression of the human retinoblastoma (rb) gene has been demonstrated to play an important role in the pathogenesis of rb and other tumors. to determine whether the rb gene might be involved in the pathogenesis of human ovarian and endometrial cancer, dna from 24 human ovarian tumors, 3 normal ovaries, 3 endometrial carcinomas, and 1 endometrial hyperplasia was examined with an rb complementary dna probe. evidence for homozygous deletion of the rb gene was observed in only one speci ...19902224770
an analysis of abnormalities of the retinoblastoma gene in human ovarian and endometrial carcinoma.the altered expression of the human retinoblastoma (rb) gene has been demonstrated to play an important role in the pathogenesis of rb and other tumors. to determine whether the rb gene might be involved in the pathogenesis of human ovarian and endometrial cancer, dna from 24 human ovarian tumors, 3 normal ovaries, 3 endometrial carcinomas, and 1 endometrial hyperplasia was examined with an rb complementary dna probe. evidence for homozygous deletion of the rb gene was observed in only one speci ...19902224770
decreased stability of dna in cells treated with alkylating agents.a modified highly sensitive procedure for the evaluation of dna damage in individual cells treated with alkylating agents is reported. the new methodology is based on the amplification of single-strandedness in alkylated dna by heating in the presence of mg2+. human ovarian carcinoma cells a2780 were treated with nitrogen mustard (hn2), fixed in methanol, and stained with monoclonal antibody (moab) f7-26 generated against hn2-treated dna. binding of moab was measured by flow cytometry with indir ...19902257876
the laboratory identification of gram-positive anaerobic cocci.a collection of 256 clinical strains and 40 reference strains of gram-positive anaerobic cocci (gpac) was studied, to characterise the recognised species more fully and to define groups of strains which might correspond to previously undescribed species. the methods used were: gas-liquid chromatography (glc) for the detection of volatile fatty acids (vfas); determination of the pre-formed enzyme profile with a commercially available kit, atb 32a; microscopic appearance; colonial morphology; and ...19912030504
decreased stability of dna in cells treated with alkylating agents.a modified highly sensitive procedure for the evaluation of dna damage in individual cells treated with alkylating agents is reported. the new methodology is based on the amplification of single-strandedness in alkylated dna by heating in the presence of mg2+. human ovarian carcinoma cells a2780 were treated with nitrogen mustard (hn2), fixed in methanol, and stained with monoclonal antibody (moab) f7-26 generated against hn2-treated dna. binding of moab was measured by flow cytometry with indir ...19902257876
antibody response in immunized rabbits measured with bacterial immunoglobulin-binding proteins.protein g, an immunoglobulin (ig)-binding protein isolated from group c or g streptococci, binds to the fc portion of igg. protein l, from the anaerobic bacterium peptostreptococcus magnus, specifically binds light chains of ig. in this study, protein g and l were used to measure the production of antibodies in immunized rabbits. two rabbits were immunized with a mixture of human urinary proteins from a patient with tubular proteinuria, and blood samples were collected regularly from the animals ...19902273256
biliary excretion of glutathione conjugates of 4,5-epoxy-4,5-dihydro-1- nitropyrene and 9,10-epoxy-9,10-dihydro-1-nitropyrene in rats administered 1-nitropyrene orally and their further metabolism in the intestinal tract.1-nitropyrene (1-np), a mutagenic and carcinogenic substance that occurs in the environment, is metabolized by reductive and oxidative pathways. 4,5-epoxy,4,5-dihydro-1-np (1-np 4,5-oxide) and 9,10-epoxy-9,10-dihydro-1-np (1-np 9,10-oxide) are oxidatively activated metabolites of 1-np, and react with glutathione. hplc analysis of biliary metabolites from rats administered [3h]1-np orally showed the presence of glutathione conjugates of 1-np 4,5-oxide and 1-np 9,10-oxide and their metabolites, cy ...19902387025
comparison of four commercial brucella agar media for growth of anaerobic organisms.four different commercial brucella blood agar plating media (anaerobe systems, bbl microbiology systems, remel, and scott laboratories) were compared for the abilities to recover anaerobic organisms from clinical specimens and to support the growth of american type culture collection anaerobic stock cultures. following 24 h of incubation in an anaerobe chamber, anaerobe systems prereduced, anaerobically sterilized brucella plates yielded 63% of the total clinical anaerobe isolates, the scott med ...19892584378
association of antisense oligonucleotides with lipoproteins prolongs the plasma half-life and modifies the tissue distribution.in order to direct antisense oligonucleotides to specific tissues or cell types in vivo, we are exploring the possibility to utilize lipoproteins as transport vehicles. a 16-mer oligonucleotide (odn) was derivatized at the 5' prime through a 32p phosphate spacer with cholesterol, yielding a 32p-labeled amphiphatic cholesteryl-oligonucleotide (cholodn). incubation of cholodn with low-density lipoprotein (ldl) for 2 hr at 37 degrees c resulted in the formation of a cholodn-ldl complex that migrate ...19911891360
[oncogenes in human leukemia].results of our study on the activation of n-ras oncogene by point mutation in human leukemia and myelodysplastic syndrome have been described in this article. point mutation was observed mainly on the 12th, 13th and 61st amino acid codon of ras genes. therefore, oligomers containing mutations at these codons were used as probes for dot blot analysis of dna derived from patient's bone marrow cells or leukemia cells. polymerase chain reaction technique was used to amplify the dna of ras genes cont ...19892650633
statin, a nonproliferation-specific protein, is associated with the nuclear envelope and is heterogeneously distributed in cells leaving quiescent state.statin, a protein of 57,000 daltons, is present primarily in the nuclei of nonproliferating cells of terminally differentiated tissues or of in vitro aged fibroblast cultures. in young growing cells, the protein can be induced to appear in the nuclei once the cell-cycle traverse is blocked by various tissue culture manipulations, such as serum starvation; this expression, however, can be rapidly removed by addition of serum. the disappearance of statin in cells leaving the quiescent state is not ...19892674158
statin, a nonproliferation-specific protein, is associated with the nuclear envelope and is heterogeneously distributed in cells leaving quiescent state.statin, a protein of 57,000 daltons, is present primarily in the nuclei of nonproliferating cells of terminally differentiated tissues or of in vitro aged fibroblast cultures. in young growing cells, the protein can be induced to appear in the nuclei once the cell-cycle traverse is blocked by various tissue culture manipulations, such as serum starvation; this expression, however, can be rapidly removed by addition of serum. the disappearance of statin in cells leaving the quiescent state is not ...19892674158
serological studies of peptostreptococci using an indirect fluorescent antibody test.members of the genus peptostreptococcus are frequent isolates from periodontal lesions. a study was undertaken for determination of the serological relationships of oral and non-oral strains of several species of this genus. eighty-nine strains of peptostreptococci representing seven species were tested by means of an indirect fluorescent antibody technique (ifa), with rabbit antisera to peptostreptococcus anaerobius atcc 27337, peptostreptococcus micros vpi 2618-a, and peptostreptococcus produc ...19892685068
bidirectional morphological changes induced by dexamethasone in morris hepatoma cell lines mca-rh7777 and mca-rh8994: independence of fibronectin and its receptor.two morris hepatoma-derived cell lines, mca-rh7777 (7777) and mca-rh8994 (8994), exhibit different alterations in morphology upon exposure to glucocorticoid. after treatment with synthetic glucocorticoid dexamethasone (dex), 7777 cells show increased adhesiveness and more flattened shape, while dex-treated 8994 cells show decreased adhesiveness to substratum and exhibit a marked increase of round and detached cells. since fibronectin has been thought to play an important role in cell adhesivenes ...19911829041
bidirectional morphological changes induced by dexamethasone in morris hepatoma cell lines mca-rh7777 and mca-rh8994: independence of fibronectin and its receptor.two morris hepatoma-derived cell lines, mca-rh7777 (7777) and mca-rh8994 (8994), exhibit different alterations in morphology upon exposure to glucocorticoid. after treatment with synthetic glucocorticoid dexamethasone (dex), 7777 cells show increased adhesiveness and more flattened shape, while dex-treated 8994 cells show decreased adhesiveness to substratum and exhibit a marked increase of round and detached cells. since fibronectin has been thought to play an important role in cell adhesivenes ...19911829041
[changes in lactate dehydrogenase activity in chicken tissues in hyperthyreosis in ontogenesis].the lactate dehydrogenase activity in reactions of lactate oxidation and synthesis was studied in subfractions of the chicken brain, heart and liver at the embryonal, early postembryonal and adult stages of development after thyroxine administration. it has been shown that during embryogenesis thyroxine predominantly enhanced the rate of lactate oxidation in the mitochondrial tissues. a marked increase in the lactate synthesis was found in cytoplasm of the adult chicken tissues. specificity of e ...19892588348
protein l: an immunoglobulin light chain-binding bacterial protein. characterization of binding and physicochemical properties.protein l, a cell wall molecule of the bacterial species peptostreptococcus magnus with affinity for immunoglobulin (ig) light chains, was isolated after solubilization of the bacterial cell walls with mutanolysin or from the culture medium by a single affinity chromatography step on human igg-sepharose. a major protein band with an apparent molecular weight of 95,000 was obtained from both sources. the protein from the growth medium was size heterogeneous. from 1 ml of packed bacteria was prepa ...19892479638
characterization of toxigenic vibrios isolated from the freshwater environment of hiroshima, japan.the occurrence and characterization of toxigenic vibrios in surface water and sediment samples of the fresh water environment of the ohta river were studied. the membrane filter, pad preenrichment technique, followed by the placement of membranes onto thiosulfate citrate-bile salt-sucrose agar, was used for the enumeration of total vibrios. qualitative examination of pathogenic vibrios was also attempted. in addition, a survey was conducted to determine the incidence of clostridium botulinum in ...19892690736
adverse effects of the use of unusual phenethylamine compounds sold as illicit amphetamine.a 40-year-old man, who had regularly taken illicit amphetamine by intra-nasal inhalation for several years without ill effects, was admitted to hospital with signs of massive adrenergic overstimulation shortly after inhaling material which he had purchased in the belief that it was amphetamine. the administration of the beta-blocker practolol produced a paradoxical increase in blood pressure. after his discharge from hospital he suffered disabling feelings of anxiety for several weeks. analysis ...19892770471
characterization of toxigenic vibrios isolated from the freshwater environment of hiroshima, japan.the occurrence and characterization of toxigenic vibrios in surface water and sediment samples of the fresh water environment of the ohta river were studied. the membrane filter, pad preenrichment technique, followed by the placement of membranes onto thiosulfate citrate-bile salt-sucrose agar, was used for the enumeration of total vibrios. qualitative examination of pathogenic vibrios was also attempted. in addition, a survey was conducted to determine the incidence of clostridium botulinum in ...19892690736
pemphigoid, pemphigus and desmoplakin as antigenic markers of differentiation in normal and tumorigenic mouse keratinocyte lines.the expression of differentiation stages in a murine epidermal cell transformation model has been investigated as a basis for studies of chemically-induced differentiation. antibodies in sera of patients with the autoimmune diseases bullous pemphigoid and pemphigus vulgaris exhibit specific reactivity to antigenic determinants of basal and spinous cells, respectively, in sections of mouse and human epidermis. in addition, spinous cells in epidermis are reactive with a mouse monoclonal antibody t ...19892691098
modulation of the interferon antiviral activity by adriamycin in human cells in culture.the antiviral activity of exogenously applied huifn-alpha was significantly reduced in cells pretreated with non-toxic concentrations of adriamycin. with low concentrations of adriamycin (up to 0.125 micrograms/ml) the effect was reversible in all cells tested. in human foreskin (bg-9) cells, that are more resistant to adriamycin toxicity, higher nontoxic concentrations of adriamycin (0.625 micrograms/ml) caused an irreversible decrease in sensitivity to huifn-alpha. under these conditions, adri ...19862423029
the effect of infectious bursal disease virus infection on local and systemic antibody responses following infection of 3-week-old broiler chickens.broiler chickens infected at 3 weeks of age with infectious bursal disease virus (ibdv) were given brucella abortus (ba) or sheep red blood cell (srbc) antigens before, during, and after the acute phase of the infection. gland of harder (gh) extracts and serum samples were used to assay local and systemic antibody titer to each antigen 7 days after antigen was administered. antibody titers to both ba and srbc antigens were lower (p less than 0.05) in gh extracts and serum of ibdv-infected broile ...19882849401
protection of mice from lethal influenza by adoptive transfer of non-neutralizing haemagglutination-inhibiting igg obtained from the lungs of infected animals treated with defective interfering virus.the lungs of c3h/hemg mice infected with a lethal dose of the influenza virus a/wsn (h1n1) contained antibody to the viral neuraminidase glycoprotein but not to the haemagglutinin (ha). when coinoculated with a life-saving amount of defective interfering (di) wsn, lungs were found to contain both anti-neuraminidase and haemagglutination-inhibiting (hi) antibodies which peaked at 3 and 5 days post-infection (p.i.), respectively. mice coinoculated with wsn and beta-propiolactone-inactivated di wsn ...19892794973
ecology of colorado tick fever. 19882849373
antiviral chemoprophylaxis for cytomegalovirus infection. 19882849602
[role of alpha-1 and alpha-2-adrenoreceptors in achieving the compensatory response of the hemostasis system to plasmin]. 19882849566
[cloning of the gpp gene of escherichia coli and the use of recbc, sbcb cells for inserting its mutant allele into the chromosomal structure].the gpp gene involved in the pppgpp conversion into ppgpp in escherichia coli cells was cloned and localized within the multicopy pbr322 plasmid. amplification of the gpp gene leads to the decline of the intracellular level of pppgpp, which implies enhanced activity of the corresponding enzyme, guanosine pentaphosphatase. to inactivate the cloned gene, a fragment of the puc4k plasmid containing the kan gene was inserted within the gpp gene. the functional chromosomal allele of the gpp gene was r ...19882849575
regulation by interleukin 2 of interleukin 2 receptors and gamma-interferon synthesis by human thymocytes: augmentation of interleukin 2 receptors by interleukin 2.the role of interleukin 2 (il 2) on the expression of il 2 receptors and on the synthesis of gamma-interferon (gamma-ifn) by human thymocytes was investigated. human thymocytes isolated from specimens obtained during cardiac surgery of infants and children were induced with one or all of the following agents: il 2, concanavalin a (con a), and 12-o-tetradecanoylphorbol 13-acetate (tpa). the expression of il 2 receptors and gamma-ifn titers were determined. the results indicate that thymocytes cul ...19852981271
peptostreptococcus magnus does not cause hematogenous infections in experimental arthroplasties.rabbits with cemented endoprostheses in the knee joints were given 1-ml intravenous injections of suspensions containing peptostreptococcus magnus (3 x 10(8) colony-forming units/ml) on three consecutive days seven weeks postoperatively. the animals were killed after another seven weeks. the bacterial challenge did not result in infection. this is in contrast to a previous study using the same experimental system with another anaerobic bacterial species, propionibacterium acnes, which caused inf ...19892924473
stimulation of the uvrabc enzyme-catalyzed repair reactions by the uvrd protein (dna helicase ii).an in vitro assay system was constructed using highly purified preparations of uvra, uvrb, uvrc, uvrd proteins and dna polymerase i, the objective being to analyse the role of uvrd protein in excision repair of uv-induced dna damage. uvrabc enzyme-initiated repair synthesis was greatly enhanced by the addition of uvrd protein to the reaction mixture. further analysis revealed that uvrd protein stimulated introduction of strand breaks in irradiated dna by uvrabc enzyme but had no effect on the dn ...19852987825
in vivo apparent peptide-receptor dissociation rate constants for arginine vasopressin analogs estimated from inhibition of rat pressor responses.apparent pressor receptor dissociation rate constants for arginine vasopressin, arginine vasotocin, oxytocin, oxypressin, and [1-deamino, 9-d-alanineamide]arginine vasopressin were estimated by the following method. two minutes after injection of a moderate dose of agonist into urethane-anesthetized rats prepared for recording mean blood pressure, a large dose of inhibitor was injected. under these conditions, in the first few moments after inhibitor injection, there should be no rebinding of th ...19872827873
antibody responses and protection in mice immunized with herpes simplex virus type 1 antigen immune-stimulating complex preparations.the formation of immune-stimulating complexes (iscoms) obtained by mixing the glycoside quil a with an antigen preparation derived from herpes simplex virus type 1 (hsv-1)-infected cell cultures using a zwitterionic detergent is described. the hsv-1 antigen preparation incorporated into iscoms elicited significantly greater antibody responses in mice than the same preparation administered together with aluminium hydroxide gel, and provided complete protection against hsv-1 or hsv-2 lethal, syste ...19892769234
a computer-aided analysis of the effect of peripheral nerve transection on tmpase activity of substantia gelatinosa rolandi. 19863020818
ig-binding bacterial proteins also bind proteinase inhibitors.protein g is a streptococcal cell wall protein with separate binding sites for igg and human serum albumin (hsa). in the present work it was demonstrated that alpha 2-macroglobulin (alpha 2m) and kininogen, two proteinase inhibitors of human plasma, bound to protein g, whereas 23 other human proteins showed no affinity. alpha 2m was found to interact with the igg-binding domains of protein g, and in excess alpha 2m inhibited igg binding and vice versa. a synthetic peptide, corresponding to one o ...19892478629
characterization of albumin-binding properties of peptostreptococcus magnus.a peptostreptococcus magnus strain demonstrated binding activity for albumin preparations from humans, mice, and dogs, but not for rabbit or bovine albumin. the albumin binding site appeared to be heat stable and of protein nature. treatment of p. magnus cells with trypsin under specified conditions enhanced this albumin binding. electron micrographs and kinetic analyses revealed that this enhancement was the result of the removal of some cell wall associated proteins leading to a higher binding ...19892766116
cloning and characterization of the high-affinity camp phosphodiesterase of saccharomyces cerevisiae.a gene, pde2, has been cloned from the yeast saccharomyces cerevisiae that, when present in high copy, reverses the phenotypic effects of ras2val19, a mutant form of the ras2 gene that renders yeast cells sensitive to heat shock and starvation. it has previously been shown that the ras proteins are potent activators of yeast adenylate cyclase. we report here that pde2 encodes a high-affinity camp phosphodiesterase that shares sequence homology with animal cell phosphodiesterases. these results t ...19863025832
nerve growth factor deprivation results in the reactivation of latent herpes simplex virus in vitro.primary sympathetic neuronal cultures were maintained for up to 5 weeks after inoculation with herpes simplex virus (hsv) without evidence of viral infection. upon deprivation of nerve growth factor, the cultures produced infectious hsv, indicating that the cultures harbored latent hsv. this study demonstrates a function of nerve growth factor in the maintenance of hsv latency.19873035230
genes coding for rna polymerase beta subunit in bacteria. structure/function analysis.the nucleotide sequence of the rpob gene of salmonella typhimurium has been determined in this work. it was compared with known sequences of the gene from other sources and the conservative regions were detected. this allowed some interesting conclusions to be made about the distribution of the functional domains in bacterial rna polymerase and about the three-dimensional structure of its beta subunit.19883056723
multiresistant shigella infections in bangladesh. 19852863461
peptostreptococcus magnus septic arthritis--a report and review of the english literature.we describe a 30-year-old female presenting with synovitis of the left 4th proximal interphalangeal joint. anaerobic cultures of the synovial membrane grew peptostreptococcus magnus. the synovitis resolved after a course of intravenous penicillin g. the role of the anaerobic bacterium peptostreptococcus magnus in joint infections is reviewed.19883060615
protein l. a novel bacterial cell wall protein with affinity for ig l chains.a novel ig-binding protein has been isolated from the surface of bacteria belonging to the anaerobic species peptococcus magnus. to solubilize the protein, peptococci were treated with different proteolytic enzymes (papain, pepsin, and trypsin) or with mutanolysin, a bacteriolytic agent known to digest the cell walls of streptococci. papain, trypsin, and mutanolysin all solubilized peptides showing affinity for radiolabeled human igg in western blot analysis. compared with papain and trypsin, mu ...19883125250
[morphological changes caused by adrenalectomy in rabbits]. 19863019628
effects of alpha-methyl-p-tyrosine on monoamines and catecholamine receptors in rat cerebral cortex and neostriatum.the effects of inhibiting the synthesis of catecholamines using alpha-methyl-p-tyrosine (alpha-mpt) were investigated in four cortical regions (cingulate, somatosensory, visual and entorhinal-piriform) as well as in the neostriatum (caudate-putamen). after acute (48 hours) treatments with alpha-mpt the endogenous na levels were significantly reduced in all regions examined. the da contents were also decreased in regions known to possess a dense dopaminergic innervation (neostriatum, cingulate an ...19883067104
chloroquine resistant plasmodium falciparum malaria in calcutta. a case report. 19883077374
infection of a prosthetic knee joint with peptostreptococcus magnus.infection of a prosthetic knee joint with peptostreptococcus magnus in an immunosuppressed patient with rheumatoid arthritis is described. the organism is a skin commensal, generally thought to be of low pathogenicity; the difficulty in making the diagnosis is emphasised.19883196085
[the blood-perfused tail artery of the rat as a model for studying spontaneous vascular tonus]. 19883215341
glycosaminoglycans in normal and atherosclerotic human coronary arteries.glycosaminoglycans (gags) were studied in normal and atherosclerotic coronary arteries of 15- to 60-year-old finnish men who had died accidentally. the gags were fractionated and quantified with electrophoretic techniques. the contents of sulfated gags (micrograms/cm2 vessel surface area) increased continuously until 20 to 30% of the vessel surface area was covered with fibrous plaques, after which they started to decrease. the largest increases were seen in chondroitin sulfates a and c and derm ...19863083156
multiregulatory element of filamentous bacteriophages. 19852989671
[morphological changes caused by adrenalectomy in rabbits]. 19863019628
surgical cannulation of a hepatic vein in dairy cows utilizing diagnostic ultrasound.use of a diagnostic ultrasonography unit in the placement of hepatic vein catheters is described. diagnostic ultrasound proved to be a safe and rapid means of identifying the hepatic venous circulation. it provided a clear visual guide in an otherwise blind surgical procedure and allowed for a precise determination of catheter placement. thus, the method could be used to advantage in other types of catheterizations. use of ultrasonography reduced the duration of surgery by 50 to 75%, thereby min ...19873294948
naturally occurring anti-band 3 antibodies and complement in phagocytosis of oxidatively-stressed and in clearance of senescent red cells.treatment of human red blood cells with diamide and opsonization with whole serum enhanced their phagocytosis by mononuclear phagocytes. opsonization of diamide-treated red cells with whole serum containing 20-100 times the physiologic concentration of naturally occurring anti-band 3 antibodies further increased the extent of phagocytosis. enhanced phagocytosis was due to an anti-band 3 mediated binding of c3b to red cells via the alternative pathway. red cell-bound anti-band 3 was slightly elev ...19883179455
neonatal naltrexone treatment: effects on sexual and exploratory behavior in male and female rats.the effect of neonatal naltrexone treatment (100 micrograms sc from day 1 to day 10) on copulatory and exploratory behavior in male and female rats was studied. in the female, neonatal naltrexone treatment enhanced copulatory (lordosis response) and exploratory behavior. an altered response to morphine was obtained; the effect of morphine on copulatory behavior was diminished while morphine's effect on exploratory activity was potentiated. the neonatal naltrexone treatment did not cause analogou ...19883252262
pulsatile secretion of growth hormone and insulin in relation to feeding in rats.in unrestrained male wistar rats chronically implanted with intracardiac catheters, blood samples were taken every 20 min throughout the 24 h of the diurnal cycle. plasma concentrations of growth hormone (gh), insulin, and glucose were measured. the pattern of food intake was continuously monitored. the existence of 3-h pulsatile cycles of gh secretion was confirmed. in addition, short bursts of insulin secretion were observed in the middle of every second gh peak-to-peak interval. food intake a ...19873318507
biologically active luteinizing hormone (lh) in klinefelter's syndrome: response to gonadotropin-releasing hormone (gnrh) and effects of testosterone undecanoate.basal and gonadotropin-releasing hormone (gnrh)-stimulated levels of biologically active and immunoreactive lh (blh and ilh) were measured in six patients with klinefelter's syndrome (ks) (mean age 24.7 years). in the same patients the diurnal rhythm of serum testosterone (t) was investigated (morning values vs. evening values). the results were compared with those obtained in ten normal young men (mean age 29.3 years). moreover, in one patient with ks we studied the effects of testosterone unde ...19873318557
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