| urease-producing species of intestinal anaerobes and their activities. | urease activities of anaerobic bacteria that constituted predominant gut flora were examined. it was demonstrated that some strains of eubacterium aerofaciens, e. lentum, and peptostreptococcus products produced urease. they were the most numerous species in human feces. all strains of bifidobacterium infantis and some strains of bacteroides multiacidus, b. bifidum, clostridium symbiosum, fusobacterium necrophorum, f. varium, lactobacillus fermentum, peptococcus asaccharolyticus, and p. prevotii ... | 1979 | 36839 |
| aerobic and anaerobic cervical flora of healthy thai women. | the bacteriologic study of the cervical flora in 50 healthy women revealed that 70% harbored mixed aerobic and anaerobic organisms. aerobic organisms alone were recovered in 15 women (30%). polymicrobial organisms were found in all but five women (in these only one aerobe was isolated per patient). the majority of patients had 1-2 anaerobes, with more than two aerobes. the common aerobes were alpha-hemolytic streptococci, staphylococcus epidermidis and lactobacilli. the most common anaerobes wer ... | 1978 | 39833 |
| preparative isolation of the cell receptor for immunoglobulin e. | | 1979 | 89114 |
| polyribonucleotide inhibition of ribonucleic acid directed deoxyribonucleic acid polymerase of mouse mammary tumor (type b) virus and simian sarcoma (type c) virus. | | 1979 | 90331 |
| the elephant man. | | 1979 | 90342 |
| effect of ethylenediaminetetraacetate on phospholipids and outer membrane function in escherichia coli. | treatment of escherichia coli k-12 strain s15, containing a normal amount of phospholipase a, with ethylenediaminetetraacetate (edta) resulted in an increase in sensitivity of the organism to actinomycin d. strain s17, a mutant deficient in both detergent-resistant phospholipase a and detergent-sensitive phospholipase a, was considerably less sensitive to the antibiotic after the treatment. both strains released lipopolysaccharide after edta treatment, indicating that this outer membrane compone ... | 1979 | 104974 |
| multiple sclerosis: immunochemical studies on the demyelinating serum factor. | sera from patients with multiple sclerosis (ms) frequently produce demyelination of central nervous system tissue cultures. the nature of the factors responsible for demyelination is not as yet clearly established. however, several authors previously reported, in in vivo and in vitro models, demyelinating activity in igg fractions isolated from sera and cerebrospinal fluid of ms patients14,42,48. we found the demyelinating activity of ms sera to be extremely labile to conventional biochemical tr ... | 1979 | 105780 |
| an unstable donor-recipient dna complex in transformation of bacillus subtilis. | in re-extracted dna obtained shortly after uptake of transforming dna by bacillus subtilis, increased amounts of donor dna radioactivity banding at the position of donor-recipient dna complex (drc) are observed in cscl gradients, if the cells are irradiated with high doses of uv prior to reextraction of the dna. qualitatively, the same phenomenon is observed if lysates of transforming cells are irradiated. uv-irradiation of lysates of competent cells to which single-stranded dna is added after l ... | 1978 | 106232 |
| the possible role of tightly bound adenine nucleotides in oxidative and photosynthetic phosphorylation. | the tightly bound nucleotides of the beff-heart mitochondrial atpase are released during cold inactivation followed by ammonium sulfate precipitation. during incubation at 0 degrees c the sedimentation coefficient (s20w) of the atpase first declines from 12.1s to 9s. prolonged incubation or precipitation with ammonium sulfate leads to dissociation of the 9s component into subunits with s20w of 3.5s. the 9s component still bears bound nucleotides which exchange more extensively and rapidly with a ... | 1975 | 127089 |
| calcium uptake and atpase activity of microsomes from heterotopically transplanted rabbit heart. | heterotopic cardiac transplantation was performed in rabbits. the calcium uptake and atpase activities of microsomal fraction were studied in three groups (group i, normal controls; group ii, early transplants; group iii, late transplants) during severe rejection as characterized histologically and electrocardiographically. group iii showed a significant decrease in calcium uptake but no difference in atpase activity compared with groups i or ii. the results demonstrate that in the rejecting tra ... | 1975 | 127353 |
| the influence of age on the wound healing of experimental myocardial infarction in rats. | in 6-weeks-old, 5-months-old and 21-months-old rats myocardial infarction was induced by coronary artery ligature. after performing the ligature the animals were administered 3h-thymidine, 3h-proline or 35s-sulphate at different times. the following parameters were determined: number of dna- and tropocollagen-synthesizing connective tissue cells at the infarction border and at the infarction site; mean silver grain density above the nuclei or cells; duration of a cycle; number of mitoses, and in ... | 1975 | 134906 |
| [influence of horizontal immersion on urinary excretion of catecholamines]. | normal man submitted to thermoneutral water immersion in horizontal position presents an increase in diuresis, natriuresis and creatininuria. noradrenalinuria and adrenalinuria are reduced, indicating a decrease in orthosympathetic activity. | 1977 | 148963 |
| effect of dexamethasone on electrolyte transport in the large intestine of the rat. | | 1978 | 149689 |
| a nuclear mutation conferring aurovertin resistance to yeast mitochondrial adenosine triphosphatase. | a single gene nuclear yeast mutant was isolated whose mitochondrial f1-atpase was resistant to the specific f1 inhibitor aurovertin. the mutant enzyme was not cross-resistant to other f1 inhibitors. the binding of aurovertin to f1 and to the two largest f1 subunits (alpha and beta) was measured by enhancement of aurovertin fluorescence. aurovertin bound to wild type f1-atpase and to its monomeric beta subunit with about the same binding constant. it failed to bind to wild type alpha subunit or t ... | 1979 | 153911 |
| letter: light induced change in rod outer segment membrane fluidity. | | 1975 | 165628 |
| steroid production by quartered and capsular rat adrenal gland in response to haemorrhage. | 60 min after rapid bleeding (1.5--2.0 per cent of b. w.) both aldosterone and corticosterone production rate by quartered rat adrenals were found to be elevated. however, no difference was observed in the rate of aldosterone and corticosterone production by capsular adrenals of sham operated and hypovolaemic rats. corticosterone production rate by decapsulated adrenals was much more higher after haemorrhage than in the control group. the same alterations could be observed incubating adrenal tiss ... | 1975 | 179800 |
| oxidation-reduction properties of several low potential iron-sulfur proteins and of methylviologen. | apparent oxidation-reduction potentials at ph 7.0 and 25 degrees c were determined using the h2-hydrogenase system with ferredoxins from the following sources: clostridium pasteurianum, -403 mv; c tartarovorum, -424 mv; c. acidi-urici, -434 mv; peptococcus aerogenes, -427 mv; chromatium d, -482 mv (ph 8.0); b. polymyxa, fd i, -377 mv, and fd ii, -422 mv; and spinach, -428 mv. the ph dependence of these values was variable, ranging from -2 to -24 mv/ph unit increase for different ferredoxins. ove ... | 1976 | 181047 |
| effectiveness of orally administered bordetella pertussis vaccine in mice. | oral administration of killed bordetella pertussis organisms to mice results in increased resistance to an intracerebral infection with virulent b. pertussis cells. the rate of survival is dependent on the dose of antigen. but besides specific systemic immunity, which is persistent over a long period, also transient non-specific resistance is increased. these effects are evidently induced without penetration of bacterial substances into the circulation. | 1976 | 182602 |
| direct assignment of the cysteinyl, the slowly exchangeable, and the aromatic ring 1h nuclear magnetic resonances in clostridial-type ferredoxins. | we have directly assigned the 1h nmr corresponding to the cysteinyl protons, the slowly exchangeable protons, and the aromatic ring protons in the 1h nmr spectrum of clostridium acidi-urici ferredoxin by isotopic labeling and 13c nmr decoupling techniques. we also show that the resonance pattern in the 8- to 20-ppm (from 2,2-dimethyl-2-sialapentanesulfonic acid) region of the 1h nmr spectra of oxidized clostridium acidi-urici, clostridium pasteurianum, clostridium perfringens, and peptococcus ae ... | 1977 | 191457 |
| determination of cooperative interaction between clusters in clostridium pasteurianum 2 (4fe-4s) ferredoxin. | the effect of reducing one 4fe-4s cluster in clostridium pasteurianum 2 (4fe-4s) ferredoxin on the reduction potential of the unreduced cluster has been investigated. while such an effect is suggested by both the x-ray structure of peptococcus aerogenes 2 (4f-4s) ferredoxin and the polypeptide conformational change on reduction present in clostridial-type 2 (4fe-4s) ferredoxins, present studies indicate that cluster-cluster cooperative interaction is not strong enough to be of functional importa ... | 1979 | 220247 |
| the class i (schiff base) fructose-diphosphate aldolase of peptococcus aerogenes. | | 1975 | 237182 |
| inherited metabolic disease: prospects for the future in both basic and clinical research. | the birth of a child with an inherited disorder is often the beginning of a life-long problem for the whole family. about 8.5% of paediatric deaths and 4.7% of paediatric hospital admissions are due to autosomal and sex-linked recessive diseases. these figures are likely to be erroneously low because of incomplete ascertainment. the inherited metabolic diseases therefore merit study on economic as well as humanitarian grounds. investigations of the disorders of purine metabolism have been conduc ... | 1977 | 272981 |
| the germicidal effect of the open air in different parts of the netherlands. | using the microthread technique the survival of escherichia coli mre 162 in open air was measured in different parts of the netherlands. the presence of bactericidal compounds (open air factor = oaf) could be demonstrated on several days and quantitated in relative units of oaf concentration. in the absence of ozone the oaf concentration was always low. in the presence of ozone the oaf concentration was dependent on wind direction. at the selected microthread exposure sites air from areas with h ... | 1977 | 321676 |
| biochemical, enzymatic, and serological differentiation of peptococcus indolicus (christiansen) sørensen from peptococcus asaccharolyticus (distaso) douglas. | peptococcus indolicus (formerly micrococcus indolicus) is an asaccharolytic anaerobic coccus that is frequently isolated from udder secretions from cases of heifer and dry-cow mastitis (summer mastitis). to facilitate better identification and its differentiation from peptococcus asaccharolyticus, a variety of biochemical, enzymatic, and serological properties were studied. seventy-nine strains of p. indolicus of bovine origin and 10 strains of p. asaccharolyticus of human origin were examined u ... | 1979 | 372218 |
| occupational chronic exposure to organic solvents. vii. metabolism of toluene in man. | | 1979 | 429070 |
| quantitation of immunoglobulins in calf serum. | | 1979 | 433126 |
| survival of anaerobic bacteria in common laboratory diluents. | the survival of six species of anaerobic bacteria was studied in simple or commercially available diluents. bacteroides fragilis and fusobacterium nucleatum showed excellent survival in all diluents including distilled water. fusobacterium mortiferum survived well in all diluents except water and water supplemented with 0.1% gelatain. clostridium perfringens survived best in phosphate-buffered saline with gelatin. peptococcus asaccharolyticus required gelatin added to the basic diluent, and stre ... | 1979 | 479359 |
| anaerobic bacteria in late infections following orthopedic surgery. | twelve patients with late anaerobic infection following hip or knee arthroplasty or tibia osteosynthesis are presented. peptococci (peptococcus asaccharolyticus, p. magnus, p. prevotii) and peptostreptococci (peptostreptococcus micros, p. anaerobius, p. parvulus) were present in nine of the cases. anaerobic gram-positive or -negative rods were found in three cases (propionibacterium acnes, actinomyces israelii, and bacteroides fragilis). antibodies against the isolated bacteria were detected in ... | 1979 | 492071 |
| differentiation of peptococcus and peptostreptococcus by gas-liquid chromatography of cellular fatty acids and metabolic products. | gas-liquid chromatographic (glc) profiles of cellular fatty acids and metabolic products were useful in identifying strains of peptococcus saccharolyticus, peptococcus asaccharolyticus, peptostreptococcus anaerobius, peptostreptococcus micros, and streptococcus intermedius. the glc results supported the recent taxonomic decision to transfer aerotolerant peptostreptococcus species to the genus streptococcus. because inconsistencies in the results prevented our differentiating peptococcus prevotii ... | 1979 | 528680 |
| studies on the mechanism of formyltetrahydrofolate synthetase. the peptococcus aerogenes enzyme. | two conflicting mechanisms have been proposed for formyltetrahydrofolate synthetase (ec 6.3.4.3). detailed studies with a clostridial enzyme support a sequential mechanism, while a stepwise mechanism with formation of a dissociable intermediate has been proposed for the peptococcus aerogenes synthetase. however, the data supporting the p. aerogenes mechanism were obtained using synthetase of questionable purity and the results supporting the mechanism could be attributed to contaminating activit ... | 1978 | 624720 |
| assignment of the cysteinyl 13c nuclear magnetic resonances and comparison of other aliphatic amino acid resonances of clostridium acidi-urici, clostridium pasteurianum, and peptococcus aerogenes ferredoxins. | 13c nmr spectra of clostridium acidi-urici, clostridium pasteurianum, and peptococcus aerogenes ferredoxins show that some 13c resonances of the aliphatic amino acid residues are shifted significantly from their corresponding resonance positions in the spectra of model polypeptides or apoferredoxin. thirteen 13c resonances are shifted into the 80- to 120-ppm (from cs2) region, and have been assigned to the cysteinyl alpha and beta carbon atoms. the remaining shifted resonances in the 120- to 190 ... | 1978 | 701285 |
| [the serological behaviour of the pyogenic anaerobes, peptococcus asaccharolyticus and p. prevotii (author's transl)]. | using rabbit antisera against whole-cell adjuvant-free antigens, the serological behaviour of ten butyrate-producing peptococcus strains, five belonging to p. asaccharolyticus and five to p. prevotii, was studied in cross-agglutination and gel-diffusion experiments. agglutination occurred in all the homologous and nearly one third of the heterologous antigen-antibody systems. cross-reactions, however, were clearly restricted to members of the same peptococcus species. | 1975 | 818602 |
| effect of ruminal lactic acid-utilizing bacteria on adaptation of cattle to high-energy rations. | heifers, unadapted to a concentrate ration, were intraruminally inoculated (1 dose) with cultures of ruminal lactic acid-utilizing bacteria or with ruminal fluid from a steer adapted to a concentrate ration. inoculation with cultures (1 l) of selenomonas ruminantium or megasphaera elsdenii did not produce better average daily weight gains or feed efficiency of heifers fed a high-energy ration for 21 days, if these values were compared with the performance of noninoculated heifers. average daily ... | 1977 | 883707 |
| structure of peptococcus aerogenes ferredoxin. refinement at 2 a resolution. | the model for ferredoxin from peptococcus aerogenes, derived from a 2.8-a resolution map, has been refined with a 2-a resolution data set. the conventional index, r, decreased from 0.449 for the initial model to 0.188 for the unconstrained one and 0.206 for the constrained one. the standard deviations of the iron and sulfur atoms are 0.04 to 0.05 a and for the oxygen, nitrogen, and carbon atoms they range from 0.14 to 0.26 a. one hundred forty-six water oxygen atoms were included in the solvent ... | 1976 | 932007 |
| long-chain fatty acids of peptococci and peptostreptococci. | the long-chain fatty acids extracted from the whole cells of 12 clinically significant species of peptococci and peptostreptococci were characterized by gas-liquid chromatography. the resulting methylated fatty acid profiles (and some unidentified compounds) of 82 strains allowed the 12 species to be separated into four groups. fifteen strains of peptostreptococcus anaerobius were placed in group i because they had a unique, prominent compound that occurred in the area where a c8 to c10 fatty ac ... | 1976 | 1002830 |
| determination of the iron-sulfur distances in rubredoxin by x-ray absorption spectroscopy. | the high intensity x-ray flux from the synchrotron radiation at the stanford synchroton radiation project has been used to study the extended x-ray absorption fine structure (exafs) of the iron-sulfur protein peptococcus aerogenes rubredoxin. absorption measurements were made from 7080 ev, which is below the k-edge of iron, to about 650 ev above the edge and structure was obtained over the entire region. by means of a model iron-sulfur compound for evaluating the phase shifts, the variation of t ... | 1975 | 1060082 |
| nh---s hydrogen bonds in peptococcus aerogenes ferredoxin, clostridium pasteurianum rubredoxin, and chromatium high potential iron protein. | results from refinement of the crystal structures of p. aerogenes ferredoxin and c. pasteurianum rubredoxin determined by x-ray diffraction show that there are 15-18 nh---s bonds in the former and six in the latter with lengths in the range 3.1-3.9 a. earlier tritium exchange experiments are consistent with the presence of these hydrogen bonds in the ferredoxin structure and show that more peptide hydrogen atoms are available for exchange in apoferredoxin than in intact ferredoxin. four types of ... | 1975 | 1061073 |
| comparative susceptibility of anaerobic bacteria to minocycline, doxycycline, and tetracycline. | the comparative susceptibility of 622 recent clinical isolates of anaerobic bacteria to minocycline, doxycycline, and tetracycline was determined by an agar-dilution technique. in addition to bacteroides fragilis, a variety of other anaerobic bacteria was resistant to achievable blood concentrations of tetracycline (55% inhibited by 6.25 mug/ml) and doxycycline (58% inhibited by 2.5 mug/ml). in contrast, minocycline was significantly more active (p < 0.05) than both doxycycline and tetracycline, ... | 1975 | 1137358 |
| lactic acid-utilizing bacteria in ruminal fluid of a steer adapted from hay feeding to a high-grain ration. | a mature, rumen-cannulated steer fed coastal bermuda grass hay for 8 weeks was adapted to a high-grain ration by stepwise increases in grain over a period of 4 weeks. the grain rations had concentrate-to-roughage ratios of 40:60, 70:30, and 85:15 and were fed for 10, 7, and 11 days, respectively. numerical estimates of lactate-utilizing bacteria in ruminal fluid of the steer the last 3 days each ration was fed were made by colony counts. lactate-utilizing bacteria were identified as megasphaera ... | 1976 | 1275348 |
| visualization and rapid quantification of autoradiographic labeling in scanning electron microscopy applied to localization of receptor sites on the surface of whole cells. | the use of backscattered electron imaging (bei) as a routine procedure for examining autoradiographic reactions in scanning electron microscopy (sem) is described. this technique allows the determination of the number of receptor sites occupied by 125i-epidermal growth factor (egf) on whole cells. the effect of 1.25 dihydroxyvitamin d3 (1,25 (oh)2d3) on the number of epidermal growth factor receptors (egf-r) in the bt 20 human mammary carcinoma cell line (which is known to possess a very high nu ... | 1992 | 1360725 |
| [asporogenic anaerobic thoracic empyema--an experimental model for anaerobic thoracic empyema in rabbits]. | sterile pleural effusion was induced by intrapleural administration of turpentine, and empyema was induced by direct intrapleural inoculation of bacteria in rabbits. experimental models of anaerobic thoracic empyema were successfully made in this study, using escherichia coli, peptostreptococcus asaccharolyticus, bacteroides fragilis, using the following methods; (1) a single bacterium inoculation of, a) e. coli, b) p. asaccharolyticus, c) b. fragilis, (2) simultaneous inoculations with an aerob ... | 1992 | 1402083 |
| protein control of iron-sulfur cluster redox potentials. | the relationship between the three-dimensional structures of iron-sulfur proteins and the redox potentials of their iron-sulfur clusters is of fundamental importance. we report calculations of the redox potentials of the [fe4s4(s-cys)4]-2/-3 couple in four crystallographically characterized proteins: azotobacter vinelandii ferredoxin i, peptococcus aerogenes ferredoxin, bacillus thermoproteolyticus ferredoxin, and chromatium vinosum high potential iron protein (hipip). our calculations use the " ... | 1992 | 1464583 |
| 2,6-dichlorophenolindophenol is a competitive inhibitor for xanthine oxidase and is therefore not usable as an electron acceptor in the fluorometric assay. | xanthine oxidase has been recognized as an important source of oxygen free radicals in ischemia-reperfusion injury. in order to study this enzyme in biological tissues, the conversion of pterin (2-amino-4-hydroxypteridine) to isoxanthopterin provides the basis for a very sensitive fluorometric assay. xanthine oxidase is typically assayed in the presence of pterin only, while an electron acceptor which replaces nad+ is used to determine the combined xanthine dehydrogenase plus xanthine oxidase ac ... | 1992 | 1563644 |
| yeast telomere repeat sequence (trs) improves circular plasmid segregation, and trs plasmid segregation involves the rap1 gene product. | telomere repeat sequences (trss) can dramatically improve the segregation of unstable circular autonomously replicating sequence (ars) plasmids in saccharomyces cerevisiae. deletion analysis demonstrated that yeast trss, which conform to the general sequence (c(1-3)a)n, are able to stabilize circular ars plasmids. a number of trs clones of different primary sequence and c(1-3)a tract length confer the plasmid stabilization phenotype. trs sequences do not appear to improve plasmid replication eff ... | 1992 | 1569937 |
| the iron-sulfur-cluster-containing l-serine dehydratase from peptostreptococcus asaccharolyticus. stereochemistry of the deamination of l-threonine. | the stereochemistry of the deamination of l-threonine to 2-oxobutyrate, catalyzed by purified l-serine dehydratase of peptostreptococcus asaccharolyticus, was elucidated. for this purpose the enzyme reaction was carried out with unlabelled l-threonine in 2h2o and in 3hoh, as well as with l-[3-3h]threonine in unlabelled water. isotopically labelled 2-oxobutyrate thus formed was directly reduced in a coupled reaction with l- or d-lactate dehydrogenase and nadh. the (2r)- or (2s)-2-hydroxybutyrate ... | 1992 | 1572371 |
| transforming growth factor beta induces myoblast differentiation in the presence of mitogens. | transforming growth factor beta 1 (tgf-beta 1) added to l6e9 rat skeletal myoblasts in mitogen-rich medium induces a rapid decrease in c-myc expression and delays progression through the g1 phase of the cell cycle. this growth inhibitory response is followed by cell commitment to terminal differentiation with elevated expression of myogenin muscle determination genes, induction of muscle-specific proteins, and formation of multinucleated myotubes. these results suggest that tgf-beta 1 may act as ... | 1992 | 1594628 |
| two-dimensional nmr investigation of iron-sulfur cluster electronic and molecular structure of oxidized clostridium pasteurianum ferredoxin. interpretability of contact shifts in terms of cysteine orientation. | a two-dimensional nmr study has been carried out on the four-iron clusters of a bacterial oxidized ferredoxin for the purpose of investigating the relationship between contact shift patterns and the orientation of the individual coordinated cysteines. the ferredoxin from clostridium pasteurianum, cpfdox, was selected because of its extensive sequence homology, and likely close structural similarity, to the crystallographically characterized ferredoxin from peptococcus aerogenes, pa fdox (adman, ... | 1991 | 1748648 |
| comparative in vitro activity of cefpodoxime against anaerobes other than bacteroides fragilis. | to assess the in vitro activity of cefpodoxime against anaerobic respiratory tract and oropharyngeal pathogens 77 strains belonging to 18 gram-negative and 7 gram-positive species were studied by means of agar dilution tests. for comparison cefuroxime, amoxicillin, amoxicillin + clavulanic acid and clindamycin were also tested. cefpodoxime was found to be active at concentrations of less than or equal to 0.125 mg/l against prevotella oralis, prevotella buccalis, prevotella bivia, porphyromonas a ... | 1991 | 1800380 |
| bidirectional morphological changes induced by dexamethasone in morris hepatoma cell lines mca-rh7777 and mca-rh8994: independence of fibronectin and its receptor. | two morris hepatoma-derived cell lines, mca-rh7777 (7777) and mca-rh8994 (8994), exhibit different alterations in morphology upon exposure to glucocorticoid. after treatment with synthetic glucocorticoid dexamethasone (dex), 7777 cells show increased adhesiveness and more flattened shape, while dex-treated 8994 cells show decreased adhesiveness to substratum and exhibit a marked increase of round and detached cells. since fibronectin has been thought to play an important role in cell adhesivenes ... | 1991 | 1829041 |
| selection, expression, and nucleotide sequencing of the glutamate dehydrogenase gene of peptostreptococcus asaccharolyticus. | the gene for the catabolic nad-linked glutamate dehydrogenase of peptostreptococcus asaccharolyticus was cloned by selection of escherichia coli for complementation of a biosynthetic defect. cloned fragments containing the gene and the p. asaccharolyticus transcription and translation signals are very highly expressed in e. coli. the nucleotide sequence of the cloned gene was determined. it codes for a polypeptide of 421 amino acids, the sequence of which is similar to those of the nadp-acceptin ... | 1991 | 1917850 |
| the partial amino acid sequence of the nad(+)-dependent glutamate dehydrogenase of clostridium symbiosum: implications for the evolution and structural basis of coenzyme specificity. | the amino acid sequence is reported for cnbr and tryptic peptide fragments of the nad(+)-dependent glutamate dehydrogenase of clostridium symbiosum. together with the n-terminal sequence, these make up about 75% of the total sequence. the sequence shows extensive similarity with that of the nadp(+)-dependent glutamate dehydrogenase of escherichia coli (52% identical residues out of the 332 compared) allowing confident placing of the peptide fragments within the overall sequence. this demonstrate ... | 1991 | 1954226 |
| [anaerobic bacteria and their role in recurrent tonsillitis]. | the presence of anaerobic bacteria in 100 palatal tonsils obtained during the tonsillectomy, was evaluated in quality and in quantity. 17 species of anaerobic bacteria were found. the most frequent were: bacteroides melaninogenicus, peptostreptococcus anaerobius, peptostreptococcus intermedius, propionibacterium, veillonella parvula and peptococcus asaccharolyticus. in most evaluations their density was 10(5)-10(6) in gram of tonsil tissue, sometimes 10(7). its role in recurrent tonsils infectio ... | 1990 | 1981606 |
| amino acid sequence and molecular modelling of a thermostable two (4fe-4s) ferredoxin from the archaebacterium methanococcus thermolithotrophicus. | the amino acid sequence of a two (4fe-4s) ferredoxin from the methanogenic bacterium methanococcus thermolithotrophicus (fdmt) has been determined. this thermostable protein comprises 60 amino acid residues (mr 6541) and two (4fe-4s) clusters chelated to the protein through the eight cysteines. fdmt contains a relatively high number of lysines [5], threonines [4] and valines [10]. the three-dimensional molecular model generated from the peptococcus aerogenes x-ray structure keeps the characteris ... | 1991 | 1986796 |
| purification and properties of an iron-sulfur-containing and pyridoxal-phosphate-independent l-serine dehydratase from peptostreptococcus asaccharolyticus. | l-serine dehydratase with a specific activity of 15 nkat/mg protein was present in the anaerobic eubacterium peptostreptococcus asaccharolyticus grown either on l-glutamate or l-serine. the enzyme was highly specific for l-serine with the lowest km = 0.8 mm ever reported for an l-serine dehydratase. l-threonine (km = 22 mm) was the only other substrate. v/km for l-serine was 500 times higher than that for l-threonine. l-cysteine was the best inhibitor (ki = 0.3 mm, competitive towards l-serine). ... | 1991 | 2065681 |
| demonstration of the heterogeneity of the kappa-opioid receptors in guinea-pig cerebellum using selective and nonselective drugs. | in guinea-pig cerebellum, saturation studies reveal that the nonselective opioid [3h]ethylketazocine has a binding capacity (r) of 6.79 pmol/g tissue which is similar to the sum of the individual r values of the mu-, delta- and kappa 1-selective opioids. conversely, the binding parameters of the nonselective opioid [3h]bremazocine are best-fitted to a two-site model (kd1 = 0.12 nm, r1 = 11.3 pmol/g tissue; kd2 = 6.03 nm, r2 = 9.09 pmol/g tissue) with an r total value of 20.3 pmol/g tissue which ... | 1990 | 2164939 |
| the biotin-dependent sodium ion pump glutaconyl-coa decarboxylase from fusobacterium nucleatum (subsp. nucleatum). comparison with the glutaconyl-coa decarboxylases from gram-positive bacteria. | membrane preparations of fusobacterium nucleatum grown on glutamate contain glutaconyl-coa decarboxylase at a high specific activity (13.8 nkat/mg protein). the enzyme was solubilized with 2% triton x-100 in 0.5 m nacl and purified 63-fold to a specific activity of 870 nkat/mg by affinity chromatography on monomeric avidin-sepharose. the activity of the decarboxylase was strictly dependent on na+ (km = 3 mm) and was stimulated up to 3-fold by phospholipids. the glutaconyl-coa decarboxylases from ... | 1990 | 2244788 |
| [anaerobic bacteria in chronic renal insufficiency: apropos of a case]. | we relate a case of chronic renal insufficiency complicated by urinary anaerobic infection caused by bacteroides vulgatus and peptococcus asaccharolyticus. | 1989 | 2489402 |
| gaspak plus versus anaerocult a--two carbon dioxide/hydrogen systems for cultivation of anaerobes. | two disposable carbon dioxide/hydrogen gas-generating systems (gaspak plus and anaerocult a) were compared by assessing growth of obligate anaerobic bacteria. eighty strains representing 28 species of anaerobic bacteria commonly occurring at various body sites were seeded onto 4 brain heart chocolate agar plates using a spiral plater; and 1 plate each was subsequently incubated in 2 anaerocult a and 2 gaspak plus systems. bacterial growth was expressed as colony-forming units per ml (cfu/ml), re ... | 1989 | 2496538 |
| structure of [4fe-4s] ferredoxin from bacillus thermoproteolyticus refined at 2.3 a resolution. structural comparisons of bacterial ferredoxins. | the structure of a low-potential ferredoxin isolated from bacillus thermoproteolyticus has been refined by a restrained least-squares method. the final crystallographic r factor is 0.204 for 2906 reflections with f greater than 3 sigma f in the 6.0 to 2.3 a resolution range. the model contains 81 amino acid residues, one [4fe-4s] cluster, and 59 water molecules. the root-mean-square deviation from ideal values for bond lengths is 0.018 a, and the mean coordinate error is estimated to be 0.25 a. ... | 1989 | 2600971 |
| [oncogenes in human leukemia]. | results of our study on the activation of n-ras oncogene by point mutation in human leukemia and myelodysplastic syndrome have been described in this article. point mutation was observed mainly on the 12th, 13th and 61st amino acid codon of ras genes. therefore, oligomers containing mutations at these codons were used as probes for dot blot analysis of dna derived from patient's bone marrow cells or leukemia cells. polymerase chain reaction technique was used to amplify the dna of ras genes cont ... | 1989 | 2650633 |
| antibody responses and protection in mice immunized with herpes simplex virus type 1 antigen immune-stimulating complex preparations. | the formation of immune-stimulating complexes (iscoms) obtained by mixing the glycoside quil a with an antigen preparation derived from herpes simplex virus type 1 (hsv-1)-infected cell cultures using a zwitterionic detergent is described. the hsv-1 antigen preparation incorporated into iscoms elicited significantly greater antibody responses in mice than the same preparation administered together with aluminium hydroxide gel, and provided complete protection against hsv-1 or hsv-2 lethal, syste ... | 1989 | 2769234 |
| model of a complex between the tetrahemic cytochrome c3 and the ferredoxin i from desulfovibrio desulfuricans (norway strain). | a three-dimensional model of an electron-transfer complex between the tetrahemic cytochrome c3 and the ferredoxin i from the sulfate-reducing bacterium desulfovibrio desulfuricans (norway strain) has been generated through computer graphics methods. the model is based on the known x-ray structure of the cytochrome and on a model of the ferredoxin that has been derived through computer graphics modeling and energy minimization methods, from the x-ray structure of the homologous ferredoxin from pe ... | 1988 | 2847143 |
| ecology of colorado tick fever. | | 1988 | 2849373 |
| antiviral chemoprophylaxis for cytomegalovirus infection. | | 1988 | 2849602 |
| the crystal structure of the three-iron ferredoxin ii from desulfovibrio gigas. | the crystal structure of oxidized ferredoxin ii from the sulfate-reducing bacterium desulfovibrio gigas has been determined and refined at 1.7 a resolution. the folding of the polypeptide chain is similar to that of the 2[4fe-4s] ferredoxin in peptococcus aerogenes, except for an extended helical segment near the c-terminus. the single [3fe-4s] cluster in d. gigas is similar to a [4fe-4s] cluster, but lacks one fe atom and is coordinated to cys-8, -14 and -50. the side chain of cys-11 is not bou ... | 1989 | 2920839 |
| regulation by interleukin 2 of interleukin 2 receptors and gamma-interferon synthesis by human thymocytes: augmentation of interleukin 2 receptors by interleukin 2. | the role of interleukin 2 (il 2) on the expression of il 2 receptors and on the synthesis of gamma-interferon (gamma-ifn) by human thymocytes was investigated. human thymocytes isolated from specimens obtained during cardiac surgery of infants and children were induced with one or all of the following agents: il 2, concanavalin a (con a), and 12-o-tetradecanoylphorbol 13-acetate (tpa). the expression of il 2 receptors and gamma-ifn titers were determined. the results indicate that thymocytes cul ... | 1985 | 2981271 |
| inhibition and activation of bovine heart nad-specific isocitrate dehydrogenase by atp. | in the absence of added calcium, inhibition of nad-specific isocitrate dehydrogenase by atp occurred without adp (i0.5 = 1.8 mm) and with 0.2 mm adp3- (i0.5 = 1.0 mm) at subsaturating substrate concentrations at ph 7.4. inhibition by atp was competitive with nad+ in the presence and absence of adp and was not reversed by magnesium citrate. no reversal of atp inhibition by free ca2+ was observed in the presence of adp (0.2 mm). however, when adp was absent, increasing ca2+ first caused progressiv ... | 1985 | 2990340 |
| [morphological changes caused by adrenalectomy in rabbits]. | | 1986 | 3019628 |
| ampicillin/sulbactam versus metronidazole-gentamicin in the treatment of soft tissue pelvic infections. | the clinical efficacy and safety of ampicillin/sulbactam versus metronidazole-gentamicin were evaluated in a comparative, randomized, prospective study. forty-four patients were enrolled: 22 received the ampicillin/sulbactam regimen, and 22 received the metronidazole-gentamicin combination. there were 33 cases of severe acute pelvic inflammatory disease, two tuboovarian abscesses, five cases of endomyometritis, and two cases of posthysterectomy pelvic cellulitis. aerobic and anaerobic cultures f ... | 1987 | 3030109 |
| the protein responsible for center a/b in spinach photosystem i: isolation with iron-sulfur cluster(s) and complete sequence analysis. | the 9 kda polypeptide from spinach photosystem i (ps i) complex was isolated with iron-sulfur cluster(s) by an n-butanol extraction procedure under anaerobic conditions. the polypeptide was soluble in a saline solution and contained non-heme irons and inorganic sulfides. the absorption spectrum of this iron-sulfur protein was very similar to those of bacterial-type ferredoxins. the amino acid sequence of the polypeptide was determined by using a combination of gas-phase sequencer and conventiona ... | 1988 | 3049567 |
| consecutive infected aneurysms caused by salmonella. | treatment of infected aneurysms consists of operation, which depends on the location and pathologic type, and antibiotic therapy, before and at least six weeks following operation. the authors present a case of two consecutive aneurysms occurring in a patient with salmonella infection. full recovery was obtained after surgical excision of the aneurysms, prolonged antibiotic therapy, and cholecystectomy. | 1988 | 3067740 |
| a new expression system for mammalian cells based on putative replicator sequences of the mouse and a truncated thymidine kinase gene. | we have constructed a new expression vector for mammalian cells. the vector contains a truncated tk gene for amplification under selective conditions, a sequence putatively supporting the replication of plasmid dna in eukaryotic cells (murine autonomously replicating sequence) and an expression cassette for the cdna to be studied. as a model cdna we have used that of human tissue-type plasminogen activator (t-pa). analysis of hirt supernatants and chromosomal dna from l cells, prepared six weeks ... | 1988 | 3149608 |
| chromium: a review of environmental and occupational toxicology. | the following topics are covered in this brief review on the environmental and occupational toxicology of chromium: occurrence, production and uses of chromium and chromium compounds; experimental toxicology; chromium toxicity for man; hygienic and ecologic aspects of chromium contamination of the environment. the review provides a conclusive evidence which suggests that chromium, especially its hexavalent form, is both toxic and carcinogenic, but its trivalent form is physiologically essential ... | 1985 | 3157743 |
| crystal structure of clostridium acidi-urici ferredoxin at 5-a resolution based on measurements of anomalous x-ray scattering at multiple wavelengths. | the crystal structure of clostridium acidi-urici ferredoxin has been determined using multiple wavelength anomalous diffraction (mad) techniques at 5.0-a resolution. the electron density map shows striking similarity to a map of peptococcus aerogenes ferredoxin computed at the same resolution from the atomic coordinates reported by adman et al. (adman, e. t., sieker, l. c., and jensen, l. h. (1973) j. biol. chem. 248, 3987-3996). such similarity is expected from the high degree of identity betwe ... | 1988 | 3192542 |
| influence of inoculation dose, inoculation schedule, chicken age, and host genetics on disease susceptibility and development of resistance to eimeria tenella infection. | various parasite- and host-related factors influencing disease susceptibility and development of protective immunity against eimeria tenella infection were investigated in two inbred strains of chickens. chickens that received a primary inoculation of 10(3), 10(4), or 10(5) oocysts showed a significant reduction in packed cell volume and produced significantly more oocysts than chickens inoculated with fewer oocysts. younger chickens were as susceptible as older chickens to identical parasite do ... | 1988 | 3264150 |
| surgical cannulation of a hepatic vein in dairy cows utilizing diagnostic ultrasound. | use of a diagnostic ultrasonography unit in the placement of hepatic vein catheters is described. diagnostic ultrasound proved to be a safe and rapid means of identifying the hepatic venous circulation. it provided a clear visual guide in an otherwise blind surgical procedure and allowed for a precise determination of catheter placement. thus, the method could be used to advantage in other types of catheterizations. use of ultrasonography reduced the duration of surgery by 50 to 75%, thereby min ... | 1987 | 3294948 |
| [meningoencephalitis and mononucleosis syndrome associated with acute infection by human immunodeficiency virus]. | | 1988 | 3347109 |
| tertiary structure of bacillus thermoproteolyticus [4fe-4s] ferredoxin. evolutionary implications for bacterial ferredoxins. | the structure of a low-potential [4fe-4s] ferredoxin from bacillus thermoproteolyticus has been solved using anomalous scattering data from iron atoms in the diffraction data of native crystals and refined partially to a crystallographic r-factor of 0.33, with 2.3 a (1 a = 0.1 nm) resolution data. the least-squares refinement based on the bijvoet differences has determined that the four iron atoms in the cluster are an equal distance, approximately 2.8 a, apart. the nh ... s hydrogen bonds betwe ... | 1988 | 3351918 |
| piezoelectric polymer curvature sensor for measurement of regional curvature radius of lv wall. | to evaluate regional myocardial function, we developed a curvature sensor for direct and instantaneous measurement of the regional curvature radius of the lv wall. the sensor is a bimorph of two sheets of thin piezoelectric polymer film. the relation between output voltage of the sensor and the reciprocal of the known curvature radius has been shown to be linear. in anesthetized dogs, we inserted the curvature sensor into the subepimyocardium with a specially designed introducer. special care wa ... | 1988 | 3364584 |
| effects of ru 486 on the mechanical properties of rabbit fallopian tubes. | | 1988 | 3377712 |
| recovery of anaerobic bacteria from clinical specimens in 12 years at two military hospitals. | examination of 15,844 clinical specimens submitted over 12 years (1973 to 1985) to the anaerobic microbiology laboratories in two military hospitals demonstrated the recovery of anaerobic bacteria in 4,458 (28.1%) specimens. the specimens yielded 6,557 anaerobic isolates (1.47 isolates per specimen). bacteroides spp. accounted for 43% of all isolates; anaerobic gram-positive cocci, 26%; clostridium spp., 7%; and fusobacterium spp., 4%. bacteroides spp. predominated in abscesses, obstetrical and ... | 1988 | 3384929 |
| evidence of estrogen receptors in normal human osteoblast-like cells. | in seven strains of cultured normal human osteoblast-like cells, a mean of 1615 molecules of tritium-labeled 17 beta-estradiol per cell nucleus could be bound to specific nuclear sites. the nuclear binding of the labeled steroid was temperature-dependent, steroid-specific, saturable, and cell type-specific. these are characteristics of biologically active estrogen receptors. pretreatment with 10 nanomolar estradiol in vitro increased the specific nuclear binding of progesterone in four of six ce ... | 1988 | 3388021 |
| structure of ferredoxin i from azotobacter vinelandii. | the structure of azotobacter vinelandii ferredoxin i (av fdi, 106 amino acids) has been redetermined, based on x-ray diffraction data from tetragonal crystals of the native protein and two heavy atom derivatives. the current model differs greatly from the one previously reported and is in agreement with arguments based on various spectroscopic and other methods. the unit cell parameters are a = b = 55.62 a and c = 95.51 a, whereas the space group was found to be p4(1)2(1)2 instead of p4(3)2(1)2. ... | 1988 | 3422475 |
| [studies on antimicrobial concentrations of flomoxef in serum, pelvic dead space exudate, and pelvic organs/tissues]. | to women undergoing radical and total hysterectomy, flomoxef (fmox, 6315-s) in a dose of 2 g was administered by intravenous drip infusion over 1 hour and drug concentrations in serum and pelvic dead space exudate as well as pelvic organs/tissues were determined over time. the following results were obtained: 1. serum concentrations of fmox after intravenous infusion showed the peak value of 92.86 +/- 17.05 micrograms/ml at the end of infusion and then gradually decreased to 29.00 +/- 10.49 micr ... | 1987 | 3444023 |
| demonstration of locally synthesized borrelia antibodies in cerebrospinal fluid. | antibodies against borrelia burgdorferi develop slowly. low titers can be anamnestic. in order to prove the etiology of manifestations of erythema chronicum migrans disease of the nervous system like meningopolyneuritis garin-bujadoux-bannwarth or progressive borrelia encephalomyelitis we used the elisa to measure specific igg antibodies against ixodes ricinus borrelia per microgram igg in serum and cerebrospinal fluid. with the mentioned method we were able to demonstrate locally synthesized an ... | 1987 | 3591093 |
| identification of anaerobic bacteria using high-performance liquid chromatography. | 20 species of the genera clostridium, bacteroides, fusobacterium and the type strains of peptostreptococcus anaerobius, peptococcus asaccharolyticus, veillonella parvula and propionibacterium acnes were examined for the production of volatile (vfa) and nonvolatile (nvfa) short-chain fatty acids using high-performance liquid chromatography (hplc) with a column for organic acids (aminex hpx-87h). 10 min are needed for sample preparation and the vfa and nvfa were detected simultaneously in a single ... | 1987 | 3673341 |
| spinal cord serotonin release and raised blood pressure after brainstem kainic acid injection. | the recently developed technique of in vivo dialysis has permitted us to make direct measurements of serotonin release in a specific region of the spinal cord and to relate this to changes in blood pressure elicited by chemical stimulation of the brainstem. in the present experiments we have shown that chemical stimulation of bulbospinal neurons in the region of the b3 cell group in the ventromedial medulla, causes an increase in the release of serotonin in the thoracic spinal cord and that this ... | 1986 | 3697690 |
| toxicity of perfluorinated fatty acids for human and murine b cell lines. | the toxicity of two perfluorinated fatty acids, penta decafluoro-n-octanoic acid (pfoa) and nonadecafluoro-n-decanoic acid (ndfda), on three mammalian b cell lines was evaluated. cells were exposed to the perfluorinated molecules for either 24 or 48 hr under a variety of culture conditions. immunoglobulin secretion and surface membrane expression were unaffected by both pfoa and ndfda at sublethal concentrations. lethal effects of pfoa and ndfda are diminished by either lowering culture temperat ... | 1986 | 3764929 |
| analysis of edta-soluble cell surface components of gram-positive anaerobic cocci. | the protein content of edta extracts from 76 strains of gram-positive anaerobic cocci was examined using sds-page. strains of peptostreptococcus anaerobius produced almost identical profiles; greater heterogeneity was observed within the species peptococcus magnus, peptococcus prevotii and peptococcus asaccharolyticus, but several strains within each biotype produced similar patterns. serological investigation of these extracts by elisa revealed numerous cross-reactions among the different bioty ... | 1986 | 3806050 |
| [polarographic method for determining activity of dehydrogenases of the pentosephosphate pathway of carbohydrate metabolism]. | a procedure is developed for estimation of glucose-6-phosphate and 6-phosphogluconate dehydrogenases activity in human erythrocytes. the procedure involved polarographic registration of oxygen consumption, which is directly related to content of nadph formed, in a coupled enzymatic system containing peroxidase and mn2+. | 1986 | 3811277 |
| moxalactam versus clindamycin plus tobramycin in the treatment of obstetric and gynecologic infections. | the clinical efficacy of moxalactam versus clindamycin/tobramycin was evaluated in a comparative, randomized, prospective study. sixty patients were treated: 30 with moxalactam and 30 with clindamycin/tobramycin. there were 15 cases of tuboovarian abscess, 36 cases of severe pelvic inflammatory disease with peritonitis, eight cases of endomyometritis, and one wound abscess. aerobic and anaerobic cultures from the sites of infection yielded 441 microorganisms from 53 patients; an average of 8.3 b ... | 1985 | 3895947 |
| peptococcaceae bacteremia. | bacteremia with peptococcaceae is an uncommon clinical manifestation of infection with this family of microorganisms. a 20-month review of 12 patients with bacteremic infections due to anaerobic gram-positive cocci revealed that obstetrical patients during the peripartum period constitute the group at greatest risk for the development of such infections. eight of the 12 patients were young women hospitalized on the obstetrical service. seven patients had postpartum endometritis and one patient h ... | 1986 | 3956135 |
| evaluation of broth microdilution susceptibility results for anaerobic organisms by use of a rapid direct colony inoculum. | a direct colony inoculum suspension procedure was compared with the overnight suspension procedure recommended for the broth microdilution anaerobic commercial system (micro-media systems, inc., potomac, md.). six national committee for clinical laboratory standards-recommended quality control organisms, bacteroides fragilis atcc 25285, clostridium perfringens atcc 13124, bacteroides thetaiotaomicron atcc 29741, bacteroides vulgatus atcc 29327, peptococcus magnus atcc 29328, peptococcus asacchar ... | 1985 | 3972999 |
| anaerobic bacteria in urine before and after prostatic massage of infertile men. | in the present study 25 infertile men delivered urine before and after prostatic massage. expressed prostatic secretion (eps) was obtained from 11 men. aerobic and anaerobic bacterial analyses showed that a total of 33 isolates was found in samples of urine voided before massage as compared to 59 isolates after massage of the prostate. there was an increase in the number of anaerobes whereas there was no change in the number of aerobes. the occurrence of eps did not influence the number of aerob ... | 1985 | 4000063 |
| amino acid sequence of the [4fe-4s] ferredoxin isolated from desulfovibrio desulfuricans norway. | the complete amino acid sequence of the [4fe-4s] ferredoxin from desulfovibrio desulfuricans norway was determined by repetitive edman degradation of the whole protein and peptides derived from tryptic digestion. the protein has 59 residues. four of the six cysteine residues are involved in the binding of the [4fe-4s] cluster in the same arrangement as in clostridial ferredoxins. this sequence is compared to various desulfovibrio ferredoxin sequences and to the sequence and three-dimensional str ... | 1985 | 4008492 |
| a sodium ion gradient as energy source for peptostreptococcus asaccharolyticus. | the determination of enzymatic activities in cell-free extracts of acidaminococcus fermentans and peptostreptococcus asaccharolyticus led to a refined scheme for the pathway of glutamate fermentation via (r)-2-hydroxyglutarate to acetate and butyrate. from the ratio of these products the amount of atp generated by substrate level phosphorylation was calculated. growth experiments with the organisms including clostridium symbiosum and clostridium tetanomorphum indicated that a sodium gradient con ... | 1985 | 4037980 |
| fluorescent antibody study of the gram-positive anaerobic cocci. | fluorescent antibody conjugates were prepared from five species of anaerobic cocci commonly isolated from human infections. when tested with homologous and heterologous cells these conjugates were found to be highly specific. there was no evidence of a common genus antigen. peptococcus magnus conjugates detected a species-specific antigen; cross-reactions with peptostreptococcus anaerobius were readily eliminated by absorption. the conjugates from peptococcus asaccharolyticus, peptococcus prevot ... | 1974 | 4140708 |
| studies on the structure of the mitochondrial membranes. | | 1970 | 4245917 |
| action of an n-acetylmuramidase from a limax amoeba on the murein of proteus mirabilis. | | 1971 | 4256271 |
| [comparative study of the convulsion provoking activity of actinomycin d and methionine sulfoximine injected into the cerebrospinal fluid of dogs]. | | 1971 | 4262072 |