| immune suppression in vivo with antigen-modified syngeneic cells. ii. t cell-mediated nonresponsiveness to fowl gamma-globulin. | intravenous administration of syngeneic spleen cells coupled with the palmitoyl derivative of fowl gammma-globulin (p-f gamma g) results in a profound state of f gamma g-specific tolerance in c57bl/6 mice. administration of p-f gamma g coupled syngeneic cells specifically reduces both the primary and secondary hapten and carrier-specific pfc responses to tnp-f gamma g. since the haptenic response is affected, the tolerance functions at the level of the f gamma g-specific helper t cell. as few as ... | 1979 | 87447 |
| enzymic polyadenylation of 5s ribosomal ribonucleic acid and synthesis of a complementary deoxyribonucleic acid. | the 5s ribosomal rna has been isolated, pure and intact, from rat liver (5 mg of 5s rna from 150g of liver). the 5s rna serves as a primer for calf thymus poly(a) polymerase with 20% of the efficiency of (ap)3a. bacterial 5s rna and transfer rna also serve as primers; rat liver 18s and 28s ribosomal rnas support poly(a) synthesis poorly. neither the 5s rna primer nor the appended poly(a) tract is nicked or degraded by poly(a) polymerase, and initiation of poly(a) tracts on 5s rna primers continu ... | 1979 | 88957 |
| the pathophysiology of asthma. | because postmortem studies of humans provide little information on the initial pathophysiologic events in asthma, animal models have been developed. recently the ascaris-allergic rhesus monkey has provided an opportunity to examine the onset of pathophysiologic changes following challenge and to correlate them with airway structure. these studies have suggested that the initial interaction between antigen and mast cells may occur in the bronchial lumen or in the epithelium superficial to the tig ... | 1979 | 89006 |
| hepatitis-a-virus exposure in the gambia. | | 1979 | 91064 |
| studies on the estrogenic activity of chlordecone (kepone) in the rat: effects on uterine estrogen receptor. | | 1979 | 91089 |
| the harada's staining method for leprosy bacilli. | | 1979 | 91361 |
| human lung tissue and anaphylaxis:cyclic nucleotide response and prostaglandin synthesis. | | 1979 | 91946 |
| [heterologous transplantation of the human medulloblastoma--with particular reference to the histogenesis of medulloblastoma (author's transl)]. | | 1979 | 91984 |
| binding of 125i-labeled proteinases to plasma proteins in cystic fibrosis. | samples of plasma or serum from 53 cystic fibrosis (cf) patients, 90 relatives of cf patients , and 159 controls have been incubated with porcine or bovine 125i-trypsin, electrophoresed on polyacrylamide gel, and autoradiographed. in these individuals, the main binding protein for 125i-trypsin has been shown to be alpha 2-macroglobulin (alpha 2m). using this method of analysis, no difference in electrophoretic migration of 125i-trypsin-alpha 2m complexes has been observed between cf and control ... | 1979 | 92005 |
| mhc-restricted cytotoxic t cells: studies on the biological role of polymorphic major transplantation antigens determining t-cell restriction-specificity, function, and responsiveness. | | 1979 | 92183 |
| processing of 19 s thyroglobulin in vitro [proceedings]. | | 1979 | 92247 |
| synthesis of an immunogen with catechol estrogen as immunodeterminant group and its characterization by the induced antibodies. | | 1979 | 92446 |
| the degradation of myelin basic protein by serum proteinase in experimental allergic encephalomyelitis and control rats. | | 1979 | 92775 |
| cytologic investigations in chlamydia infection. | chlamydia is an extremely common genital infection. it can cause serious disseminated disease and can infect the neonate and the sexual partner. morphologically, chlamydia infection was identified in routine fast smears obtained from 160 women. chlamydia infection manifests as intracytoplasmic coccoid and inclusion bodies in the metaplastic cells. infected cells also reveal cytomegaly and multinucleation. immunofluorescence studies, electron microscopy, tissue studies, histochemical techniques a ... | 1979 | 93839 |
| effect of allopurinol on pyrimidine metabolism in human white blood cells. role of the salvage pathway. | | 1979 | 94760 |
| hemolytic streptococci in supramammary lymph nodes from healthy pigs from the peruvian jungle. | hemolytic streptococci were isolated from 53 percent (18) supramammary lymph nodes from healthy adult pigs raised at the peruvian jungle. the isolates were identified as streptococcus zooepidemicus, streptococcus infrequens, streptococcus faecalis and groups p and s. the possible significance of the findings are discussed. | 1978 | 101478 |
| experimental pyelonephritis in the monkey. vi. infection of infants versus adults. | pyelonephritis followed ureteral inoculation of bacteria in both infant and adult monkeys. because of the frequency of reflux in infants this was done by bladder inoculation, although ureteral inoculation was necessary in adults. the longer duration of bacteriuria in infants may be attributable to a relative immunodeficiency. | 1978 | 101479 |
| familial hyperphosphatasemia: diagnosis in early infancy and response to human thyrocalcitonin therapy. | familial hyperphosphatasemia is an uncommon hereditary disorder of membranous bone with concurrent overproduction and overdestruction of bone and bone collagen by osteocytes. this process does not allow normal maturation into compact lamellar bone. two cases of severely affected children are presented which demonstrate that the condition can be diagnosed in early infancy by abnormalities in the long bones. at this stage the skull may appear normal and the characteristic thickening of the calvari ... | 1979 | 106682 |
| raised intracranial pressure: its management in neisseria meningitidis meningoencephalitis. | further reduction in morbidity and mortality from bacterial meningitis is unlikely to stem from innovations in antibiotic management. emphasis must therefore be directed toward prevention, more rapid diagnosis, and improved therapy for maintaining the integrity of the cns. raised intracranial pressure is frequently associated with bacterial meningitis. in selected patients with bacterial meningitis and clinical evidence of elevated intracranial pressure, monitoring and aggressive treatment of th ... | 1979 | 106721 |
| [the treatment of hepatic emergencies]. | hepatic emergency means impending or manifest hepatic insufficiency. it is caused by acute and even more often by chronic liver diseases. the patient has to be hospitalized for treatment. basic treatment is of utmost importance. it can be done in every intensive-care unit: careful monitoring and treatment of any complication as well as reduction of toxic protein degradation products by protein restriction, bowel emptying, and enteral application of lactulose and/or neomycin. the infusion of spec ... | 1979 | 107099 |
| primary structure of a human iga1 immunoglobulin. iv. streptococcal iga1 protease, digestion, fab and fc fragments, and the complete amino acid sequence of the alpha 1 heavy chain. | in order to establish the complete amino acid sequence of the human iga alpha1 chain bur, iga1 protease from streptococcus sanguis was employed to generate fabalpha and fcalpha fragments in the final stage of this investigation. cyanogen bromide cleavage of the fabalpha fragment followed by reduction and aminoethylation produced the fd' fragment (residues 84 to 227); this contains part of the variable region (vr), the whole first constant domain (calpha1), and part of the hinge region of this he ... | 1979 | 107164 |
| [laboratory diagnosis of group d streptococcus]. | after taxonomic listing of group d streptococci, the authors discuss the methodology of the bacteriologic diagnosis of infections produced by these bacteria, showing the way in which the pathological products are collected, the strains isolated and seeded. the strains are identified in two stages: (1) group identification by group antigen identification, resistance to oxacillin, development at +45 degrees, development on bilaesculin media; (2) species and variety identification by biochemical, p ... | 1979 | 111334 |
| [quantitative changes in the cellular makeup of the spleen in mice infected with the rauscher leukemia virus and brucella abortus]. | changes in the absolute number of splenic cells of mice infected with rauscher leukemia virus (rlv) and a day later--with living brucella (ba) were studied. the increase in the absolute number of splenic cells of mice infected with rlv + ba was initially caused by the lymphoid tissue reaction to brycella and then by the development of leukemia. the number of reticular cells in the spleen appeared to be larger than the control (rlv) within all observation periods; the number of basophilic normocy ... | 1979 | 111416 |
| the radiographic diagnosis of heart disease in the dog. | | 1975 | 123974 |
| staphylococcal micrococcins. i. isolation of antibiotic-producing strains. | seven strains belonging to the family micrococcaceae and possessing strong antagonistic properties against staph. aureus and several other gram-positive bacteria, have been isolated. the strains were characterized and identified, phage typed, tested for antibiotic-sensitivity and antagonistic activities. two patients of inhibition were noted. | 1975 | 126688 |
| spasticity: actions of selectivity activated group ii muscle spindle afferents on extensor motoneurons. | | 1975 | 129880 |
| [importance of hygiene in the prevention of infectious atrophic rhinitis]. | | 1975 | 129905 |
| [chemotherapy of swine dysentery]. | | 1975 | 129937 |
| functional differences of ouabain and ethacrynic acid on renal potassium metabolism in dogs. | current concepts provide inadequate explanations for the effects of ethacrynic acid (eca) and ouabain (oua) on renal potassium metabolism because neither the site of action, nor the tubular effects have been agrreed upon. in the present study on anaesthetized dogs, in vivo inhibitory effects of eca on mitochondria or na-k-atpase were excluded. firstly, eca hardly reduced outer medullary metabolic rates in kidneys exposed to oua and 2,4-dinitrophenol. secondly, whereas oua reduced renal na-katpa ... | 1978 | 152453 |
| satellite association in human metaphases. a comparative study of normal individuals, patients with down syndrome and their parents. | | 1979 | 154489 |
| effect of a mild exercise program on myocardial function and the development of hypertrophy. | cardiac function and the development of myocardial hypertrophy were studied in rats conditioned by an exercise program consisting of 8 wk of running on a treadmill. at the end of the training period a group of exercised and sedentary rats was subjected to hemodynamic evaluation under general anesthesia. except for a slight elevation in the heart rates of the exercised animals there were no significant differences between the exercised and sedentary rats at rest. following an increase in afterloa ... | 1979 | 154493 |
| deoxyribinucleic acid-binding proteins in virus-transformed cell lines. | the synthesis of proteins with affinity for dna has been studied in clones of a syrian hamster cell line (nil) and subclones of this line transformed by polyoma virus (nil-py) or hamster sarcoma virus (nil-hsv). the results show that the synthesis of dna-binding proteins in nil and in its virus-transformed derivatives nil-py and nil-hsv is very similar in exponentially growing cells, but in dense culture there is a very significant difference in the level of a protein (p8), which is much higher ... | 1975 | 165180 |
| actions and interactions of theophylline and imidazole on cardiac contractility, phosphorylase activation and cyclic amp. | injection of 1.0 mg theophylline and 1.6 mg imidazole into guinea-pig hearts perfused by the langendorff technique increased cardiac contractile force by 20.0 +/- 1.0% and 23.3 +/- 1.2% over control and activated phosphorylase without producing significant changes in cyclic amp levels. when guinea-pig hearts were perfused with theophylline (10(-3) m) or imidazole (20 mm), injection of either imidazole or theophylline did not further increase cardiac contractility or phosphorylase a levels. in al ... | 1976 | 183614 |
| herpesvirus-induced urolithiasis in specific-pathogen-free male cats. | | 1977 | 201200 |
| distribution of corticotrophin releasing factor activity within the hypothalamic-pituitary complex of rats and cattle. | an assay system involving cultured rat adenohypophysial cells from either intact or adrenalectomized donors was used to study the distribution of corticotrophin releasing factor (crf) activity in the hypothalamic-pituitary complex of rats and cattle. in the rat hypothalamus, crf activity was most concentrated in the median eminence, but crf was present in the stalk and the posterior pituitary gland in much higher concentrations than in the median eminence in both species. the dose--response slop ... | 1977 | 201710 |
| cell membrane structure of human giant-celled glioblastoma. | a giant-cell glioblastoma was examined by electron microscopy and by the freeze-fracture technique. the cell membranes bordering the extensive extracellular space often showed complicated undulations and peripheral vacuoles as well as occasional microvilli or filopodia. the undulations were mainly composed of plasmalemmal vesicles as well as of large (400--800 nm in diameter) and small (30--50 nm in diameter) localized protrusions and invaginations of the cell membrane. deep invaginations of the ... | 1978 | 205080 |
| characterization of a unique cell line (laz 221) from human acute lymphocytic ("null" cell) leukemia. | a unique human cell line designated laz 221 has been established from the peripheral blood of a patient with acute lymphocytic leukemia of the "null" cell type. the cell line does not possess the epstein-barr virus nuclear antigen and has a karyotype of 45,xx,-9,-12,+(9q12q). both the established cell line and the patient's uncultured blast cells share the same phenotypic markers. they both lack t-cell markers. they fail to form sheep erythrocyte rosettes and do not react with t-cell-specific an ... | 1978 | 205365 |
| common mesophilic anaerobes, including clostridium botulinum and clostridium tetani, in 21 soil specimens. | a relatively rich medium was markedly superior to a dilute medium for the isolation of anaerobic bacteria from soil. the obligate anaerobes isolated from 21 soil samples were all clostridia and the counts ranged from 2.7 x 10-2 to 3.3 x 10-6 per g. the organisms most frequently isolated were clostridium subterminate, c. sordelii, c. sporogenes, c. indolis, c. bifermentans, c. mangenoti, and c. perfringens. seventeen other species were also recognized but almost one-third of the isolates could no ... | 1975 | 238468 |
| isoelectric focusing of proteins using a ph gradient created by a concentration gradient of nonelectrolytes in solution. | a new method of producing a stable ph gradient in buffer solutions is suggested, obtained by the concentration gradient of a nonelectrolyte in buffer solutions as a result of the gradual change in the dielectric properties of the solution. the maximal concentrations of nonelectrolyte which do not influence the protein configuration allow a ph gradient with a range of two ph units to be produced. it is suggested that the properties of some polyols (i.e. glycerol or mannitol) be used to change the ... | 1975 | 238655 |
| insulin resistance due to a defect distal to the insulin receptor: demonstration in a patient with leprechaunism. | we have studied a 2-year-old girl with acanthosis nigricans, glucose intolerance, marked hyperinsulinemia, and somatic features characteristic of the leprechaunism syndrome. circulating plasma insulin levels were increased up to 50-fold and the patient showed a blunted hypoglycemic response to an injection of exogenous insulin (0.2 units/kg), indicating the presence of severe insulin resistance. insulin purified from the patient's plasma was normal on the basis of chromatographic, electrophoreti ... | 1978 | 277948 |
| the lipid composition of rat brain aggregating cell cultures during development. | the lipid and fatty acid composition of rat brain was studied during its development both in vivo and in an aggregating cell culture system. although the amount of lipid present in the cultures was very low, the increase in glycolipid content corresponded closely to the period of intense myelin formation. very long chain fatty acids (hydroxylated and unsubstituted) were present in 41-day cultures. in comparison to the in vivo situation, myelination was delayed in vitro and, after 40 days in cult ... | 1979 | 514541 |
| effects of ca+ and k+ on oscillatory afterpotentials in dog ventricular muscle fibers. | | 1979 | 522140 |
| [the total suppression of the external pancreatic secretion of the dog (author's transl)]. | | 1979 | 544203 |
| use of adeno-associated virus as a general transduction vector for mammalian cells. | | 1992 | 1316261 |
| tert-butyl hydroperoxide-induced radical production in rat liver mitochondria. | when rat liver mitochondria are treated with tert-butyl hydroperoxide (tbhp) in the presence of the spin trap 5,5-dimethyl-1-pyrroline n-oxide (dmpo), electron paramagnetic resonance (epr) signals are detected attributable to spin adducts resulting from the trapping of methyl, tert-butoxyl, and tert-butylperoxyl radicals. the addition of respiratory substrate results in a 3- to 7.5-fold increase in the signal intensity of the dmpo/methyl adduct, no change in the signal intensity of the dmpo/tert ... | 1992 | 1317326 |
| on the mechanism of action of picrotoxin on gaba receptor channels in dissociated sympathetic neurones of the rat. | 1. the mechanism of action of picrotoxin on gaba receptor channels in rat sympathetic neurones has been investigated with whole-cell clamp. in addition, the action of picrotoxin on single gaba channels has been examined in outside-out membrane patches from these cells. 2. picrotoxin, at concentrations which dramatically reduced the amplitude of whole-cell gaba currents, did not alter the spectral time constants or single-channel conductance estimated by analysis of gaba-activated current noise. ... | 1992 | 1317428 |
| concentrations of endothelial-cell-stimulating angiogenesis factor, a major component of human uterine angiogenesis factor, in human and bovine embryonic tissues and decidua. | embryonic development involves the establishment of new patterns of vascular growth in the fetus and within the lining of the womb. a factor, human uterine angiogenesis factor, has been purified from the decidua and stimulates the growth of blood vessels in collagen sponge implants and in the chick chorioallantoic membrane. evidence is presented that suggests that a major active component of human uterine angiogenesis factor is an activator of latent matrix metalloproteinases, of low m(r), calle ... | 1992 | 1317450 |
| reaction mechanism of glutamate racemase, a pyridoxal phosphate-independent amino acid racemase. | glutamate racemase of pediococcus pentosaceus contained no cofactor, and was completely inactivated by a thiol reagent. the role of a cysteine residue in the enzyme reaction was studied by chemical modification. the modification of this cysteine residue resulted in a concomitant loss of activity. dl-glutamate protected the enzyme from inactivation. the inactivated enzyme was reactivated by addition of dithiothreitol. the racemization in 2h2o showed an overshoot in the optical rotation of glutama ... | 1992 | 1358877 |
| effects of magnesium on cocaine-reinforced responding in mice, rats and squirrel monkeys. | | 1992 | 1435977 |
| pyomyositis and cryptococcal sepsis in an hiv-infected patient with severe granulocyte defect. | | 1992 | 1472345 |
| transforming growth factor beta and basic fibroblast growth factor synergistically promote early bovine embryo development during the fourth cell cycle. | developmentally competent bovine blastocysts were produced by adding transforming growth factor beta (tgf beta) and basic fibroblast growth factor (bfgf) to serum-free cultures of in vitro produced, 2-cell bovine embryos. the effects of tgf beta were evaluated because this growth factor signals synthesis and secretion of the extracellular matrix component fibronectin and its receptor. previous investigations have demonstrated that fibronectin promotes early bovine embryo development in vitro. th ... | 1992 | 1472373 |
| the effects of na(+)-k(+)-cl- co-transport and cl(-)-hco3-exchange blockade on the membrane potential and intracellular chloride levels of rat arterial smooth muscle, in vitro. | membrane potential and intracellular chloride levels have been measured simultaneously in vascular smooth muscle from the femoral artery of the rat, in vitro, using double-barrelled chloride-sensitive microelectrodes. these values are compared in the presence and absence of bicarbonate, and inhibitors of cl(-)-hco3- exchange and na(+)-k(+)-cl- co-transport. both dids and bumetanide caused a hyperpolarization of membrane potential (em) and a fall in [cl-]i, in the presence of extracellular hco3-. ... | 1992 | 1489543 |
| cd45 isoform expression on human neonatal t cells: expression and turnover of cd45 isoforms on neonatal versus adult t cells after activation. | neonatal t cells are phenotypically similar to "naive" t cells from adult donors in the cd45 isoform expression. despite the phenotypic similarity, large differences were found between neonatal and adult t cells when t cells were activated. after activation with pha, adult cd45ra+ t cells began to express cd45ro and no loss of cd45ra expression had yet occurred at day 3 post-stimulation. three days after activation, cd45ra+ neonatal t cells also coexpressed cd45ro; however, in contrast to adult ... | 1992 | 1534035 |
| intrauterine resuscitation in the severely distressed fetal dog. | severe fetal distress was produced in 16 fetal dogs by successive and/or prolonged occlusion of the maternal abdominal aorta in an acute surgical preparation, and was characterized in fetal arterial blood of 7.06 for ph, 10 mm hg for o2, 127 mm hg for co2, and fetal heart rate decelerations. five fetuses recovered spontaneously in utero and delivered normally; 3 needed maternal oxygenation before delivery; 3 suddenly died in utero; 5 fetuses were delivered while still alive in utero when the tph ... | 1992 | 1563650 |
| pharmacokinetic interaction between 1,3-butadiene and styrene in sprague-dawley rats. | gas uptake studies were carried out to evaluate kinetic interactions between 1,3-butadiene and styrene in sprague-dawley rats. the animals were co-exposed by inhalation to a mixture of 1,3-butadiene between 20 and 6000 ppm (v/v) and styrene between 0 and 500 ppm. the data demonstrate that metabolism of 1,3-butadiene was partially inhibited by styrene. the inhibition was competitive at atmospheric concentrations of styrene up to 90 ppm. higher concentrations of styrene resulted in a small additio ... | 1992 | 1610292 |
| headspace flavour compounds produced by yeasts and lactobacilli during fermentation of preferments and bread doughs. | production of volatile flavour compounds during fermentation with pure cultures of saccharomyces cerevisiae and candida guilliermondii, lactobacillus brevis and lactobacillus plantarum have been investigated, using wheat doughs and several preferements as substrates. for yeast, preferments consisted of 10% (w/v) glucose, maltose and sucrose solutions, whereas for lactobacilli they consisted of supplemented and unsupplemented (3% and 10% (w/v)) glucose solutions, and a 10% (w/v) wheat flour slurr ... | 1992 | 1622751 |
| conduction velocity distribution of afferent fibers innervating the rat urinary bladder. | the conduction velocities of individual afferent fibers innervating the rat urinary bladder were determined by the antidromic stimulation of dorsal roots while recording from bladder postganglionic nerves. conduction velocities ranged from 0.5 to 21.0 m/s; 70% of the velocities were less than 2.5 m/s. the distribution within the dorsal roots was ipsilateral with 84% in l6 and 16% in s1. neuroanatomical tracing with horseradish peroxidase applied to individual bladder postganglionic nerves result ... | 1990 | 1698508 |
| septicaemia caused by pediococcus pentosaceus: a new opportunistic pathogen. | a case of septicaemia caused by pediococcus pentosaceus is described. the role played by pediococci, and other vancomycin-resistant gram-positive cocci, in disease states is examined. we suggest that in immunocompromised patients these organisms act as opportunist pathogens. this would appear to be the first reported case of p. pentosaceus septicaemia. | 1991 | 1753117 |
| recovery of vancomycin-resistant gram-positive cocci from pediatric liver transplant recipients. | between november 1988 and october 1989, 49 first-time pediatric liver transplant recipients at the children's hospital of pittsburgh were prospectively monitored for the presence of stool colonization and the development of disease caused by vancomycin-resistant gram-positive cocci (vrgpc). quantitative stool culturing was done on a weekly basis, and cultures were planted onto a selective medium for vrgpc. isolates for which the mic was greater than or equal to 8 were considered resistant to van ... | 1991 | 1774255 |
| bacteriocin-mediated inhibition of clostridium botulinum spores by lactic acid bacteria at refrigeration and abuse temperatures. | the bacteriocinogenicity of lactococcus lactis atcc 11454, pediococcus pentosaceus atcc 43200, p. pentosaceus atcc 43201, lactobacillus plantarum bn, l. plantarum lb592, l. plantarum lb75, and lactobacillus acidophilus n2 against clostridium botulinum spores at 4, 10, 15, and 35 degrees c was investigated by modified deferred and simultaneous antagonism methods. all the strains, except l. acidophilus n2, produced inhibition zones on lawns of c. botulinum spores at 30 degrees c. l. plantarum bn, ... | 1991 | 1785919 |
| endotoxin induces eosinophil accumulation in the rat pleural cavity. | changes in eosinophil counts after intrathoracic (it) injection of endotoxin (lps) were investigated in wistar rats (150-180 g). increasing doses of endotoxin (62.5-500 ng/cavity) induced a dose-dependent increase in the number of eosinophils recovered from the rat pleural cavity 24 h later. the eosinophilia was apparent within 24 h, peaked within 48 h (from 0.76 +/- 0.12 to 3.68 +/- 0.51 eosinophils x 10(6)/cavity, p less than 0.001) and returned to basal levels 120 h after the it injection of ... | 1991 | 1797292 |
| endotoxin induces eosinophil accumulation in the rat pleural cavity. | changes in eosinophil counts after intrathoracic (it) injection of endotoxin (lps) were investigated in wistar rats (150-180 g). increasing doses of endotoxin (62.5-500 ng/cavity) induced a dose-dependent increase in the number of eosinophils recovered from the rat pleural cavity 24 h later. the eosinophilia was apparent within 24 h, peaked within 48 h (from 0.76 +/- 0.12 to 3.68 +/- 0.51 eosinophils x 10(6)/cavity, p less than 0.001) and returned to basal levels 120 h after the it injection of ... | 1991 | 1797292 |
| effects of the glycine prodrug milacemide (2-n-pentylaminoacetamide) on catecholamine secretion from isolated adrenal medulla chromaffin cells. | 1. milacemide (2-n-pentylaminoacetamide) is a glycine prodrug which readily crosses the blood brain barrier and increases brain glycine and glycineamide. in vitro and in vivo studies, with numerous tissues, including adrenal chromaffin cells, have clearly shown that the formation of the latter metabolites is exclusively mediated by monoamine oxidase b for which milacemide is a substrate. 2. milacemide, glycineamide and glycine caused a time- and dose-dependent release of catecholamines from bovi ... | 1991 | 1797336 |
| effects of the glycine prodrug milacemide (2-n-pentylaminoacetamide) on catecholamine secretion from isolated adrenal medulla chromaffin cells. | 1. milacemide (2-n-pentylaminoacetamide) is a glycine prodrug which readily crosses the blood brain barrier and increases brain glycine and glycineamide. in vitro and in vivo studies, with numerous tissues, including adrenal chromaffin cells, have clearly shown that the formation of the latter metabolites is exclusively mediated by monoamine oxidase b for which milacemide is a substrate. 2. milacemide, glycineamide and glycine caused a time- and dose-dependent release of catecholamines from bovi ... | 1991 | 1797336 |
| intravenous hypertonic saline induces fos immunoreactivity in neurons throughout the lamina terminalis. | expression of fos, the protein product of c-fos, was studied immunohistochemically in the forebrain of rats infused intravenously with hypertonic solutions. intravenous 1.5 or 0.75 mol/l nacl or 1.2 mol/l sucrose in 0.15 mol/l nacl, but not isotonic 0.15 mol/l nacl, caused increased fos expression in the hypothalamic paraventricular and supraoptic nuclei and throughout the lamina terminalis (organum vasculosum laminae terminalis, median preoptic nucleus and subfornical organ). these results show ... | 1991 | 1797341 |
| intravenous hypertonic saline induces fos immunoreactivity in neurons throughout the lamina terminalis. | expression of fos, the protein product of c-fos, was studied immunohistochemically in the forebrain of rats infused intravenously with hypertonic solutions. intravenous 1.5 or 0.75 mol/l nacl or 1.2 mol/l sucrose in 0.15 mol/l nacl, but not isotonic 0.15 mol/l nacl, caused increased fos expression in the hypothalamic paraventricular and supraoptic nuclei and throughout the lamina terminalis (organum vasculosum laminae terminalis, median preoptic nucleus and subfornical organ). these results show ... | 1991 | 1797341 |
| comparative anatomical distribution of 5-ht1a receptor mrna and 5-ht1a binding in rat brain--a combined in situ hybridisation/in vitro receptor autoradiographic study. | the present study examined the comparative distribution of 5-ht1a receptor mrna and 5-ht1a receptors in rat brain using a combination of in situ hybridisation histochemistry and in vitro receptor autoradiography. 5-ht1a mrna was visualized using a 910 bp crna probe synthesised from a bali-pvuii fragment of the rat 5-ht1a reetor gene, while 5-ht1a receptors were labelled with the 5-ht1a-selective ligand 8-oh-dpat. in general terms, there was a complementary distribution of cells expressing 5-ht1a ... | 1991 | 1797349 |
| comparative anatomical distribution of 5-ht1a receptor mrna and 5-ht1a binding in rat brain--a combined in situ hybridisation/in vitro receptor autoradiographic study. | the present study examined the comparative distribution of 5-ht1a receptor mrna and 5-ht1a receptors in rat brain using a combination of in situ hybridisation histochemistry and in vitro receptor autoradiography. 5-ht1a mrna was visualized using a 910 bp crna probe synthesised from a bali-pvuii fragment of the rat 5-ht1a reetor gene, while 5-ht1a receptors were labelled with the 5-ht1a-selective ligand 8-oh-dpat. in general terms, there was a complementary distribution of cells expressing 5-ht1a ... | 1991 | 1797349 |
| overproduction of glutamate racemase of pediococcus pentosaceus in escherichia coli clone cells and its purification. | we previously isolated a 6.0-kb dna fragment that specifies glutamate racemase activity from the chromosomal dna of pediococcus pentosaceus by digestion with hindiii (n. nakajima, k. tanizawa, h. tanaka, and k. soda, 1986), agric. biol. chem. 50, 2823-2830). we digested it further with ecori to obtain a fragment of 1.8 kb, which was blunt-ended and ligated into the smai site of vector plasmid pkk223-3. the recombinant plasmid showed a high glutamate racemase activity upon transformation of esche ... | 1991 | 1821778 |
| regulation and glucocorticoid-independent induction of lipocortin i in cultured astrocytes. | stimulation of prostaglandin (pg) release in rat astroglial cultures by various substances, including phorbol esters, melittin, or extracellular atp, has been reported recently. it is shown here that glucocorticoids (gcs) reduced both basal and stimulated pgd2 release. hydrocortisone, however, did not inhibit atp-, calcium ionophore a23187-, or tetradecanoyl phorbol acetate (tpa)-stimulated arachidonic acid release, and only tpa stimulations were affected by dexamethasone. gc-mediated inhibition ... | 1991 | 1828830 |
| [3h]mk-801 binding to n-methyl-d-aspartate receptors solubilized from rat brain: effects of glycine site ligands, polyamines, ifenprodil, and desipramine. | the n-methyl-d-aspartate (nmda) receptor is thought to contain several distinct binding sites that can regulate channel opening. in the present experiments, the effects of ligands for these sites have been examined on [3h]mk-801 binding to a soluble receptor preparation, which had been passed down a gel filtration column to reduce the levels of endogenous small-molecular-weight substances. glycine site agonists, partial agonists, and antagonists gave effects similar to those observed in membrane ... | 1991 | 1828832 |
| cyclophellitol: a naturally occurring mechanism-based inactivator of beta-glucosidases. | the natural product cyclophellitol, isolated from the culture filtrate of a mushroom, phellinus sp. is found to be a highly specific and effective irreversible inactivator of beta-glucosidases. it inactivates the beta-glucosidases from both almond emulsin and agrobacter sp. according to pseudo-first order kinetics with inactivation constants of ki = 0.34 mm, ki = 2.38 min-1, and ki = 0.055 mm, ki = 1.26 min-1 respectively. no reactivation of the inactivated enzyme is seen upon dialysis, thus pro ... | 1991 | 1904221 |
| the effect of pediococcus damnosus and pediococcus pentosaceus on the growth of pathogens in minced meat. | the antibacterial effects of one strain of pediococcus damnosus and two strains of pediococcus pentosacaeus against clostridium perfringens, listeria monocytogenes, salmonella infantis and yersinia enterocolitica were investigated. growth inhibition studies were conducted in juice from minced meat incubated at +6 degrees c and +15 degrees c for various periods after the inoculation with pediococci. inhibitory effects were seen for all bacteria tested. | 1991 | 1907475 |
| the occurrence and growth of microorganisms during the fermentation of fish sausage. | minced fish (mullet) sausage mixes containing added sugar, salt, nitrate, nitrite and spices were fermented (48 h, 30 degrees c) by indigenous flora or by a starter culture (pediococcus acidilactici) and the microbial ecology and behaviour of various bacteria was monitored. pediococcus pentosaceus and lactobacillus plantarum dominated the indigenous fermentation, achieving populations of 10(7)-10(8) cfu/g by 48 h, and decreasing the ph of the mix to 4.5-4.7. significant growth (10(5)-10(7) cfu/g ... | 1991 | 1909546 |
| the effect of prophylaxis with chloroquine and proguanil on delayed-type hypersensitivity and antibody production following vaccination with diphtheria, tetanus, polio, and pneumococcal vaccines. | in vitro studies have shown that anti-malarial drugs suppress immunity. in this study, the effects of chloroquine and proguanil (paludrine) on the cellular and humoral immune system were measured by two in vivo methods: 1) cell-mediated immunity (delayed cutaneous hypersensitivity) i.e., skin tests with seven delayed-type common antigens (multitest) and 2) humoral immunity by measurement of specific antibody response to vaccination. sixty healthy young individuals were randomized into four group ... | 1991 | 1951872 |
| use of dna probes in the study of silage colonization by lactobacillus and pediococcus strains. | a technique to monitor lactic acid bacteria inoculants in silage, based on specific dna probes, was developed and used to evaluate the colonization properties of two strains of lactobacillus plantarum and one strain of pediococcus pentosaceus which were used as maize silage inoculants in farm conditions. the results indicated that these three strains were able to dominate the natural microflora of the silage, representing more than the 95% of the bacterial biomass of the maize silage. these stud ... | 1991 | 1960106 |
| oxidized low density lipoproteins inhibit relaxations of porcine coronary arteries. role of scavenger receptor and endothelium-derived nitric oxide. | we studied the effects of low density lipoprotein (ldl) on endothelium function. | 1991 | 2040054 |
| probing the coenzyme specificity of glyceraldehyde-3-phosphate dehydrogenases by site-directed mutagenesis. | by combining our knowledge of the crystal structure of the glycolytic nad-dependent glyceraldehyde-3-phosphate dehydrogenase (gapdh) and the sequence of the photosynthetic nadp-dependent gapdh of the chloroplast, two particular amino acid residues were predicted as the principal determinants of differing coenzyme specificity. by use of site-directed mutagenesis, the amino acids leu 187 and pro 188 of gapdh from bacillus stearothermophilus have been replaced with ala 187 and ser 188, which occur ... | 1990 | 2223764 |
| probing the coenzyme specificity of glyceraldehyde-3-phosphate dehydrogenases by site-directed mutagenesis. | by combining our knowledge of the crystal structure of the glycolytic nad-dependent glyceraldehyde-3-phosphate dehydrogenase (gapdh) and the sequence of the photosynthetic nadp-dependent gapdh of the chloroplast, two particular amino acid residues were predicted as the principal determinants of differing coenzyme specificity. by use of site-directed mutagenesis, the amino acids leu 187 and pro 188 of gapdh from bacillus stearothermophilus have been replaced with ala 187 and ser 188, which occur ... | 1990 | 2223764 |
| chlamydial igg and iga in serum and follicular fluid among patients undergoing in vitro fertilisation. | the point prevalence of igg and iga antibodies to chlamydia was analyzed in serum and follicular fluid in 63 patients undergoing in vitro fertilisation (ivf) in comparison to sera of 298 healthy women by the single serovar (l2) inclusion immunoperoxidase assay (ipa). the presence of specific igg and iga to chlamydia in follicular fluid was demonstrated. no statistical association was found between the presence of specific chlamydia igg and iga in serum and follicular fluid to oocyte fertilizatio ... | 1990 | 2242796 |
| cloning and sequence analysis of mink growth hormone cdna. | a cdna clone for mink growth hormone (gh) was isolated from a mink pituitary cdna library, employing a part of rat growth hormone cdna sequence as a probe. according to the nucleotide sequence, mature mink gh consists of 190 amino acids with a calculated molecular weight of 21,720. the amino acid sequence homology between the mature region of mink gh and those of pig gh, rat gh, bovine gh and human gh was 98.4%, 93.7%, 89.0% and 66.7%, respectively. | 1990 | 2268323 |
| aminooxy analogues of spermidine as inhibitors of spermine synthase and substrates of hepatic polyamine acetylating activity. | aminooxy analogues of spermidine, 1-aminooxy-3-n-[3-aminopropyl]- aminopropane (ap-apa) and n-[2-aminooxyethyl]-1,4-diaminobutane (aoe-pu), were tested as substrates or inhibitors of the enzymes involved in methionine and polyamine metabolism. both compounds were good competitive inhibitors and poor substrates of spermine synthase, good substrates of cytosolic polyamine acetyltransferase, inactivators of s-adenosylmethionine decarboxylase and inhibitors of ornithine decarboxylase. ap-apa and aoe ... | 1990 | 2292587 |
| mapping the effects of motor cortex stimulation on somatosensory relay neurons in the rat thalamus: direct responses and afferent modulation. | single unit recordings were used to map the spatial distribution of motor (mi) cortical influences on thalamic somatosensory relay nuclei in the rat. a total of 215 microelectrode penetrations were made to record single neurons in tracks through the medial and lateral ventroposterior (vpm and vpl), ventrolateral (vl), reticular (nrt), and posterior (po) thalamic nuclei. single units were classified according to their: 1) location within the nuclei, 2) receptive fields, and 3) response to standar ... | 1990 | 2322860 |
| mapping the effects of motor cortex stimulation on somatosensory relay neurons in the rat thalamus: direct responses and afferent modulation. | single unit recordings were used to map the spatial distribution of motor (mi) cortical influences on thalamic somatosensory relay nuclei in the rat. a total of 215 microelectrode penetrations were made to record single neurons in tracks through the medial and lateral ventroposterior (vpm and vpl), ventrolateral (vl), reticular (nrt), and posterior (po) thalamic nuclei. single units were classified according to their: 1) location within the nuclei, 2) receptive fields, and 3) response to standar ... | 1990 | 2322860 |
| bismuth subsalicylate in the prevention of colonization of infant mice with campylobacter jejuni. | infant mice were used for the evaluation of the efficacy of bismuth subsalicylate (bss) in the prevention of the growth of campylobacter jejuni in the intestine. the mic90 of ten c. jejuni strains was 900 micrograms/ml. of three dosage regimens tested, continuous treatment before and after the bacterial challenge, mimicking the way bss is used in the prevention of traveller's diarrhoea, was the most effective. growth inhibition was dose dependent; the high dose of 2000 micrograms per day was mor ... | 1990 | 2347383 |
| heme-dependent and heme-independent nitrite reduction by lactic acid bacteria results in different n-containing products. | seventy strains of lactic acid bacteria were investigated for nitrite reductase activity. two types of this activity were detected. type i was found in lactobacillus plantarum, l. pentosus and pediococcus pentosaceus. this activity is heme-dependent with ammonia as the sole product. type ii mechanism is heme-independent and reduces nitrite to no and n2o. | 1990 | 2397159 |
| initiation and termination of human u1 rna transcription requires the concerted action of multiple flanking elements. | sequences in the 5' flanking region of small nuclear rna (snrna) genes are responsible for recognition of 3' end signals. formation of the pre-u1 3' end occurs at the downstream signal closest to the promoter, probably by transcription termination. we have analyzed promoter elements for their participation in formation of the 3' ends of pre-u1 rna. to do this, a human u1 rna gene with deletions in individual promoter elements was microinjected into xenopus laevis oocytes and the resulting rnas w ... | 1989 | 2587258 |
| an enzyme-linked immunosorbent assay for the quantitation of dna. | | 1989 | 2607159 |
| control of escherichia coli populations by a combination of indigenous clostridia and lactobacilli in gnotobiotic mice and continuous-flow cultures. | the function of indigenous lactobacilli in the control of other intestinal microbial species is not clear. still more controversial is the effect of dietary bacterial supplements containing lactobacilli or other species. this situation is unlikely to change unless the mechanisms that control the colonization of ingested bacteria are better understood, and until more detailed information becomes available on the mechanisms by which certain populations of indigenous bacteria can affect the populat ... | 1989 | 2643576 |
| rational drug therapy based on understanding the pathophysiology of hypertension. | increased peripheral vascular resistance is the hemodynamic hallmark of chronic hypertension. evidence suggests that ca2+ is vital in mediating vasoconstrictive mechanisms. calcium channel blockers correct the specific arteriolar vasoconstrictive mechanism without unwanted compensatory actions. human primary hypertension may also be related to volume variations in which renal influences contribute to elevated arterial pressure and diuretics may be necessary to control the hypertension. patients ... | 1989 | 2663225 |
| identification of gram-positive coccal and coccobacillary vancomycin-resistant bacteria. | a total of 84 of 150 vancomycin-resistant (defined as no inhibition of bacterial growth around a 30-micrograms vancomycin disk placed on 5% sheep blood-trypticase soy agar [bbl microbiology systems, cockeysville, md.]) bacteria were definitively identified by determining the phenotypic criteria. the identity of representatives was also confirmed by dna-dna hybridizations. the following strains were identified: 1 enterococcus faecium, 18 leuconostoc mesenteroides, 15 leuconostoc citreum, 9 leucon ... | 1989 | 2723037 |
| tetrahydroaminoacridine and other allosteric antagonists of hippocampal m1 muscarine receptors. | tetrahydroaminoacridine (tha) and a variety of other nonclassical antagonists of muscarine receptors were studied for their ability to bind to primary and allosteric sites on muscarine receptors in rabbit hippocampal membranes. competition curves between 13 antagonists and 1 nm [3h]pirenzepine (kd = 3 nm) were simple mass action curves, but tha produced steeper curves, indicating positive cooperativity. nonetheless, tha inhibited the binding of low concentrations of [3h]pirenzepine, [3h]n-methyl ... | 1989 | 2725474 |
| sympathetic uveitis after giant tear repair. | a 33-year-old hispanic man developed sympathetic ophthalmia 4.5 months after vitrectomy and repeat scleral buckling for a giant retinal tear in an aphakic previously traumatized eye. the patient had gray-white, subretinal nodules with characteristic changes of sympathetic uveitis detected by fluorescein angiography. the fluorescein angiographic and clinical findings revealed a dramatic response to steroid treatment: visual acuity returned to the previous 20/20 level within 5 weeks of therapy. | 1989 | 2772419 |
| opioids and behavior: genetic aspects. | three animal models, based on genetic differences in endogenous opioid peptides and opioid receptors, are described. obese mice and rats, whose pituitary opioid content is elevated, may be used to investigate eating disorders. recombinant inbred strains of mice, which differ in brain opioid receptors and analgesic responsiveness, can be used for study of opioid- and nonopioid-mediated mechanisms of pain inhibition. individual reactivity to opioids can be examined in c57bl/6 and dba/2 inbred stra ... | 1988 | 2837410 |
| molecular cloning of rat phosphoprotein phosphatase 2a beta cdna and increased expressions of phosphatase 2a alpha and 2a beta in rat liver tumors. | a cdna clone coding for an isotype of the catalytic subunit of rat phosphoprotein phosphatase 2a was isolated. the deduced amino acid sequence of the clone was different at 8 positions from that of rat phosphatase 2a alpha determined in a previous study. the deduced amino acid sequence of the clone was, however, identical to that of human phosphatase 2a beta and differed only at one position from that of rabbit 2a beta. thus, the isolated cdna was identified as a clone coding for rat phosphatase ... | 1988 | 2849437 |
| hypotensive effects after topical and intra-arterial administration of alpha-adrenergic agonists in isolated perfused rabbit eyes. | the influence of nonselective and selective alpha-adrenergic agonists on intraocular pressure (iop) was studied in isolated arterially perfused rabbit eyes. drugs were given intra-arterially or topically. with either route of administration, no initial hypertensive phase was observed after l-epinephrine, l-norepinephrine, l-phenylephrine (alpha 1), or methoxamine (alpha 1), but there was an immediate ocular hypotensive effect. the alpha 2-agonist b-ht 920 caused a nonsignificant 1 mm hg fall in ... | 1985 | 2866480 |
| [adult t-cell leukemia associated with htlv1 and positive hiv2 serology in an african woman]. | we report a case of acute adult t-cell leukemia associated with htlv1 infection in a young african woman. the leukemic proliferation consisted of cd4-, cd25-positive lymphoid cells with typically convoluted nuclei and monoclonal integration of the htlv1-proviral dna. the clinical course was characterized by a rapid progression of the leukemia with development of hypercalcemia, resistance to chemotherapy, and the presence of multiple opportunistic infections. the additional finding of a seroposit ... | 1988 | 2898753 |
| comparison of toxic effects of methylguanidine, guanidinosuccinic acid and creatinine in rats with adenine-induced chronic renal failure. | methylguanidine (mg), guanidinosuccinic acid (gsa) and creatinine (cr), which accumulate in the body in parallel with the progress of renal failure after adenine administration, were given separately to rats in order to compare their toxicities. food containing adenine was given to rats for 24 days to induce renal failure, and then each of the test substances was administered intraperitoneally from the following day, the survival rates of the rats being subsequently determined. administration of ... | 1989 | 2918950 |
| cellular effects of hematoporphyrin derivative photodynamic therapy on normal and neoplastic rat bladder cells. | hpd is known to localize in neoplastic cells and when exposed to the appropriate wavelength of light causes cytotoxicity. the authors have established a rat urothelial cell model for use in comparing and contrasting the effects of hpd photodynamic therapy (pdt) in normal (rbl-01) and transitional cell carcinoma (ay27) bladder cell lines. uptake, toxicity, and morphologic damage following exposure to hpd pdt were evaluated. trypan blue exclusion was used for determination of the toxicity of sever ... | 1986 | 2936252 |
| structural and serological heterogeneity of gamma/delta t cell antigen receptor expression in thymus and peripheral blood. | monoclonal antibodies (mab) reactive against the gamma/delta t cell antigen receptor (tcr) have been used to characterize the distribution and structural properties of gamma/delta tcr-bearing lymphocytes in blood and thymus. consistent with prior reports the tcr gamma/delta-1 and delta-1 mab react with all gamma/delta tcr+ t lymphocytes in blood and thymus. by contrast the tcs-delta mab was found only to react with a subset of the gamma/delta tcr-bearing t cell population. several lines of evide ... | 1988 | 2975598 |