| microorganisms and characteristics of laban. | laban had a titratable acidity of about 1.0%, a ph of 4.25, an ethanol content of 1.25%, and contained 4.2 mug acetaldehyde and 34 mug acetoin/ml. there was no diacetyl. five microorganisms, classified as streptococcus thermophilus, lactobacillus acidophilus, leuconostoc lactis, kluyveromyces fragilis, and saccharomyces cerevisiae, were responsible for the fermentation. streptococcus thermophilus and l. acidophilus were responsible for acid production with s. thermophilus producing acid more rap ... | 1976 | 2630 |
| immunofluorescence testing for chlamydial antibodies. | | 1979 | 91742 |
| human adenohypophyseal quantitative histochemical cell classification. i. morphologic criteria and cell type distribution. | recent advances in immunocytochemistry and histochemical staining have made it possible to identify pituitary cells that contain specific hormones by their appearance under light microscopy. criteria for identification of functional cell classes were formulated from these studies. fourteen hypophyses were obtained at the time of autopsy (four to 22 hours postmortem), embedded in paraffin, sectioned, and differentially stained. cell counts on areas in the acidophil wing and basophil wedge were pe ... | 1979 | 92296 |
| [lipopolysaccharide study of the cell membranes of bacteria in the genus pseudomonas. v. the serological characteristics]. | | 1979 | 92749 |
| changes of basic biochemical indices in rat liver tissue after intraperitoneal application of endotoxin. | | 1979 | 92874 |
| specificity of serovar-specific main antigens of leptospiras shown by the inhibition of leptospiral microscopic agglutination. | | 1979 | 93155 |
| immunobiologic studies in hepatoma-bearing mice passively immunized to alpha-fetoprotein. | mice bearing the bw7756 hepatoma were passively immunized using rabbit antiserum to murine alpha-fetoprotein (afp) administered in constant or increasing doses. control tumor-bearing mice were inoculated with saline or nonimmune rabbit serum (nrs) (constant or increasing doses), or were left untreated. the tumor growth curves from mice receiving constant or increasing doses of anti-afp or constant doses of nrs showed suppression of hepatoma growth; but in both groups of anti-afp-treated mice thi ... | 1979 | 94991 |
| histochemical characteristics of the anal sacs of the cat. | the histochemistry of polysaccharides and proteins and the distribution of rna were investigated in the glands and mature secretion of the anal sacs of the cat. glycogen was extremely abundant in the epithelial lining of the lumen and efferent duct of the anal sac and also in ducts of sebaceous glands of the sacs constantly formed in the place of degenerating acini. little glycogen was found in the unchanged sebaceous acini. the apocrine glands of the anal sacs were substantially richer in glyco ... | 1979 | 95391 |
| [some aspects relating to the aflatoxin generation during the self-heating of cereals]. | in a stored batch of grain which was already affected by mould-formation tests were carried out with the known aflatoxin producer aspergillus flavus. a significantly lower aflatoxin production ensued if the mould growth was not connected with self-heating of the stored product. however, in conformity with increasing mould formation the germinating power was adversely affected and the significant signs (fatty acid number, reductive and none-reductive sugars) were influenced in the grain, irreleve ... | 1978 | 98933 |
| chemosynthetic, photosynthetic, and cyanobacterial ribulose bisphosphate carboxylase. | | 1978 | 106835 |
| the radiographic diagnosis of heart disease in the dog. | | 1975 | 123974 |
| hydrophobic interaction of alkanes with liposomes and lipoproteins. | human serum lipoproteins and egg yolk lecithin liposomes are able to solubilize large amounts of n-hexane and n-octane. at the maximum water solubility of n-octane the mole ratio of alkane to lipoprotein was 65 for high density lipoprotein (holo-hdl) and 900 for low density lipoprotein (holo-ldl). alkane binding to lipid-free apo-hdl is negligible compared to alkane solubility in holo-hdl. alkane solubility in the lipoproteins and liposomes is thermodynamically consistent with the simple soution ... | 1975 | 165199 |
| sodium periodate stimulation of human lymphocytes. a comparison between normal and chronic lymphocytic leukemia lymphocytes. | | 1975 | 169137 |
| diet, ddt, and the toxicity of drugs and chemicals. | the toxic and carcinogenic effects of many compounds depend on their activation to reactive molecules in the cytochrome p-450 system of the endoplasmic reticulum of cells. changes in dietary input alter p-450 levels in different tissues and so alter toxicity. in this way, low protein diets protect against carbon tetrachloride poisoning. fats, proteins, and nonnutrients such as flavones, antioxidants, and contaminants like ddt all affect p-450 levels. the activated molecules may be diverted from ... | 1977 | 321252 |
| [ability of yeast from the genus candida to assimilate sugars and acetic acid from mineral medium and sulfite liquors]. | | 1975 | 1214648 |
| hypoxic-ischemic damage in the neonatal brain: excitatory amino acids. | perinatal brain damage is a major clinical problem. recent studies suggest that excitatory amino acids (eaas) may be important for the development of hypoxic-ischemic brain injury in the newborn. experimental work demonstrates that the immature brain is hypersensitive to the toxic effects eaa ('excitotoxicity'), hypoxic-ischemia is accompanied by an extracellular overflow of eaas and hypoxic-ischemic brain damage is reduced by eaa receptor antagonists. clinical investigations demonstrate the pre ... | 1992 | 1306803 |
| conduction velocity distribution of afferent fibers innervating the rat urinary bladder. | the conduction velocities of individual afferent fibers innervating the rat urinary bladder were determined by the antidromic stimulation of dorsal roots while recording from bladder postganglionic nerves. conduction velocities ranged from 0.5 to 21.0 m/s; 70% of the velocities were less than 2.5 m/s. the distribution within the dorsal roots was ipsilateral with 84% in l6 and 16% in s1. neuroanatomical tracing with horseradish peroxidase applied to individual bladder postganglionic nerves result ... | 1990 | 1698508 |
| recovery of vancomycin-resistant gram-positive cocci from children. | a cross-sectional survey of vancomycin-resistant gram-positive cocci (vrgpc) in the feces of children was initiated after several bacteremic infections with these organisms occurred at our hospital. a selective medium consisting of colistin-nalidixic acid agar, 5% sheep blood, vancomycin (5 mg/liter), and amphotericin b (8 mg/liter) was developed to isolate vrgpc. a single stool specimen submitted to the clinical microbiology laboratory from each of 48 patients was inoculated onto the medium. pl ... | 1990 | 2108993 |
| characterization of leuconostoc lactis strains from human sources. | an examination of 23 vancomycin-resistant, streptococcuslike isolates of clinical origin revealed a group of 14 to be related to leuconostoc lactis (type strain, cip 102422) on the basis of chemotaxonomic studies. these isolates were initially shown to be atypical by classical biochemical tests. however, they were characterized in particular by their polar lipid patterns by thin-layer chromatography and, additionally, by whole-cell protein patterns by sodium dodecyl sulfate-polyacrylamide gel el ... | 1990 | 2394800 |
| identification of gram-positive coccal and coccobacillary vancomycin-resistant bacteria. | a total of 84 of 150 vancomycin-resistant (defined as no inhibition of bacterial growth around a 30-micrograms vancomycin disk placed on 5% sheep blood-trypticase soy agar [bbl microbiology systems, cockeysville, md.]) bacteria were definitively identified by determining the phenotypic criteria. the identity of representatives was also confirmed by dna-dna hybridizations. the following strains were identified: 1 enterococcus faecium, 18 leuconostoc mesenteroides, 15 leuconostoc citreum, 9 leucon ... | 1989 | 2723037 |
| [extraintestinal isolation of salmonella in chickens: epidemiologic study of 3 salmonellosis outbreaks after ingestion of raw eggs]. | a taxonomic analysis of the malolactic microflora present in nine grape samples from different zones of galicia, was carried out. nineteen strains were isolated and identified as lactobacillus plantarum (42%), l. brevis (10.6%), l. casei (5.25%), l. hilgardii (5.25%), streptococcus cremoris (5.25%), leuconostoc lactis (5.25%), l. oenos (10.6%), and pediococcus acidilactici (15.8%). | 1987 | 3077329 |
| [taxonomic study of epiphyte lactic bacteria in grapes in galicia]. | a taxonomic analysis of the malolactic microflora present in nine grape samples from different zones of galicia, was carried out. nineteen strains were isolated and identified as lactobacillus plantarum (42%), l. brevis (10.6%), l. casei (5.25%), l. hilgardii (5.25%), streptococcus cremoris (5.25%), leuconostoc lactis (5.25%), l. oenos (10.6%), and pediococcus acidilactici (15.8%). | 1987 | 3268295 |
| molecular aspects of genetic instability of an artificial 68 bp perfect palindrome in escherichia coli. | an artificial 68 bp perfect palindrome carried on a plasmid (pas807) is genetically unstable. an increase in the population of cells harbouring palindrome-deleted pas807 derivatives (pas807-v) is observed as the number of cell generations increases. the calculated frequency of palindrome excision events per cell generation and per plasmid replication round in escherichia coli is 0.95 x 10(-4). sequence analysis of eight independent isolates of palindrome-deleted molecules, reveals two symmetrica ... | 1987 | 3302612 |
| protective effect of prostacyclin on postischemic acute renal failure in the rat. | infusion of prostacyclin (pgi2) reportedly attenuates renal ischemic injury in the dog and the rat. in the dog, pgi2 is a potent renal vasodilator; in the rat a direct action on the renal vasculature is not always apparent. to determine whether or not the protective effect of pgi2 on postischemic arf was hemodynamically mediated, studies were performed in uninephrectomized sprague-dawley rats before and after a 40 minute period of complete renal artery occlusion. in response to the preischemic i ... | 1987 | 3323594 |
| [separation and purification of d lactate dehydrogenase and glucose-6-phosphatate dehydrogenase from leuconostoc lactis. study of several properties]. | | 1973 | 4150428 |
| immunosuppressive effect of serum from cba mice made tolerant by the subernatant from ultracentrifuged bovine gamma globulin. | | 1970 | 4194309 |
| [correlation of tuberculin sensitivity before and after bcg vaccination]. | | 1967 | 4235702 |
| effect of growth of walker 256 carcinosarcoma of the fibrinolytic system of the rat plasma and urine. | | 1970 | 4248380 |
| studies on the lysosomes of l132 cells infected with either rhinovirus type 2 or poliovirus type 1. | | 1974 | 4362408 |
| oral biology in japan 1973-1974. 2. dental histology. | | 1974 | 4620494 |
| studies on chemotaxis. vi. specific chemotaxis in rabbit polymorphonuclear leucocytes and mononuclear cells. | | 1967 | 4860339 |
| glucose production and utilization in the newborn puppy. | | 1970 | 4911571 |
| studies on liver regeneration. ii. effects of phenobarbital on the onset and pattern of rat liver regeneration following partial hepatectomy. | | 1971 | 4947050 |
| purification of alpha-acetolactate synthase from leuconostoc lactis ncw1. | | 1995 | 7672395 |
| identification and characterization of the insertion element is1070 from leuconostoc lactis nz6009. | a novel insertion sequence, designated is1070, was identified on the lactose plasmid of leuconostoc lactis nz6009 by nucleotide sequence analysis. the 1027-bp sequence contains partially matched (24 of 28 bp) inverted repeats and has one long open reading frame. the deduced 305-amino-acid sequence demonstrated homology to transposases of is30 from escherichia coli, is4351 from bacteroides fragilis, is1086 from alcaligenes eutrophus, is1161 from streptococcus salivarius, isas2 from aeromonas salm ... | 1995 | 7698675 |
| partial characterization of an rpod-like gene of lactococcus lactis subsp. lactis ml3 with a polymerase chain reaction-based approach. | with degenerated oligonucleotide primers for conserved regions of bacterial sigma factor proteins, a 117-bp internal dna fragment of an rpod-like gene of lactococcus lactis subsp. lactis ml3 was amplified by the polymerase chain reaction (pcr). the dna sequence of this pcr product was determined by cycle sequencing, and the deduced amino acid sequence of this internal fragment showed an extensive homology with the known sigma factor sequences from six other microorganisms and present a 13-amino ... | 1993 | 7764136 |
| cloning and expression of the plasmid encoded beta-d-galactosidase gene from a lactobacillus plantarum strain of dairy origin. | the beta-galactosidase (beta-gal) gene from lactobacillus plantarum c3.8 was cloned and expressed in lactococcus lactis and escherichia coli. hybridization experiments indicated that the gene is located on a plasmid and is present in other strains of lactobacillus plantarum. its sequence is very similar to a leuconostoc lactis beta-gal gene. expression of the gene, both in lactobacillus plantarum and in lactococcus lactis, was four-fold higher in cells growth in lactose compared to those grown i ... | 1994 | 7958766 |
| [microbiological and clinical studies of vancomycin resistant leuconostoc spp. and pediococcus spp. isolated from septicemia patients]. | we described three septicemia cases in which blood cultures yielded gram-positive cocci identified as leuconostoc spp. and pediococcus spp. patients were three male adults aged 63 to 71 years with severe underlying diseases, pancreatic cancer, esophageal cancer and diabetes mellitus with chronic renal failure. they had fever and chills at the onsets of septicemia with acute obstructive suppurative cholangitis, acute pneumonia, and infection complicated with invasion sites of esophageal cancer co ... | 1994 | 7963799 |
| the lactose transporter in leuconostoc lactis is a new member of the lacs subfamily of galactoside-pentose-hexuronide translocators. | the gene encoding the lactose transport protein (lacs) of leuconostoc lactis nz6009 has been cloned from its native lactose plasmid, pnz63, by functional complementation of lactose permease-deficient escherichia coli mutants. nucleotide sequence analysis revealed an open reading frame with the capacity to encode a protein of 639 amino acids which had limited but significant identity to the lactose transport carriers (lacs) of streptococcus thermophilus (34.5%) and lactobacillus bulgaricus (35.6% ... | 1996 | 8633855 |
| mechanism of cyclosporin a-induced inhibition of prostacyclin synthesis by macrophages. | in vitro studies of pg production over a 24 h period by adherent rat peritoneal macrophages activated by serum-opsonized zymosan revealed that csa (0.3-10 micrograms/ml) caused a dose-related inhibition of pgi2 (assayed as 6-oxo-pgf1 alpha) formation. indomethacin (ind, 0.01-10 micrograms/ml) and dexamethasone (dex, 0.01-10 micrograms/ml) also inhibited the pg production in a dose-related manner. when arachidonic acid (10 micrograms/ml) was added together with the inhibitors, there was no change ... | 1985 | 3936119 |
| production of menaquinones by lactic acid bacteria. | lactic acid bacteria were examined for their ability to produce quinone compounds, which may include dietary sources of menaquinones. isoprenyl quinones in bacterial cells grown in a synthetic medium were extracted and analyzed by thin layer chromatography. lactococcus lactis ssp. cremoris (three strains), lactococcus lactis ssp. lactis (two strains), and leuconostoc lactis were selected as high producers of quinone that synthesized more than 230 nmol of quinones/g of dried cells. the quinones w ... | 1999 | 10509247 |
| mechanism of cyclosporin a-induced inhibition of prostacyclin synthesis by macrophages. | in vitro studies of pg production over a 24 h period by adherent rat peritoneal macrophages activated by serum-opsonized zymosan revealed that csa (0.3-10 micrograms/ml) caused a dose-related inhibition of pgi2 (assayed as 6-oxo-pgf1 alpha) formation. indomethacin (ind, 0.01-10 micrograms/ml) and dexamethasone (dex, 0.01-10 micrograms/ml) also inhibited the pg production in a dose-related manner. when arachidonic acid (10 micrograms/ml) was added together with the inhibitors, there was no change ... | 1985 | 3936119 |
| inactivation of pseudomonas iron-superoxide dismutase by hydrogen peroxide. | pseudomonas fe-superoxide dismutase (superoxide:superoxide oxidoreductase, ec 1.15.1.1) is inactivated by hydrogen peroxide by a mechanism which exhibits saturation kinetics. the pseudo-first-order rate constant of the inactivation increased with increasing ph, with an inflection point around ph 8.5. two parameters of the inactivation were measured in the ph range 7.8 to 9.0; the total h2o2 concentration at which the enzyme is half-saturated (k inact) was found to be independent of ph (30 mm) an ... | 1986 | 3768375 |
| the management of health care workers' accidental parenteral exposures to biological specimens of hiv seropositive individuals. | health care workers can reduce risks associated with caring for hiv seropositive individuals by strict adherence to routine infection control procedures. educating personnel in infection control should be increasingly stressed in all health-related settings. when accidents occur, however, the health care industry must respond with appropriate care and follow-up of their employees. the outlined approach for the management of health care workers with needlestick injuries from hiv-contaminated biol ... | 1987 | 3647943 |
| nitrogenase of klebsiella pneumoniae. kinetic studies on the fe protein involving reduction by sodium dithionite, the binding of mgadp and a conformation change that alters the reactivity of the 4fe-4s centre. | the kinetics of reduction of indigocarmine-dye-oxidized fe protein of nitrogenase from klebsiella pneumoniae (kp2ox) by sodium dithionite in the presence and absence of mgadp were studied by stopped-flow spectrophotometry at 23 degrees c and at ph 7.4. highly co-operative binding of 2mgadp (composite k greater than 4 x 10(10) m-2) to kp2ox induced a rapid conformation change which caused the redox-active 4fe-4s centre to be reduced by so2-.(formed by the predissociation of dithionite ion) with k ... | 1987 | 3318808 |
| a patient with an interstitial deletion of the proximal portion of the long arm of chromosome 4. | a patient with a proximal deletion of the long arm of chromosome 4 is presented. this patient and 6 others previously described appear to have similar findings of moderate to severe developmental delay, small size, small hands and feet, and similar facial appearance. these patients appear to be quite different from those with more distal 4q deletions. | 1988 | 3067576 |
| bacillus subtilis mutant succinate dehydrogenase lacking covalently bound flavin: identification of the primary defect and studies on the iron-sulfur clusters in mutated and wild-type enzyme. | succinate dehydrogenase consists of two protein subunits and contains one fad and three iron-sulfur clusters. the flavin is covalently bound to a histidine in the larger, fp, subunit. the reduction oxidation midpoint potentials of the clusters designated s-1, s-2, and s-3 in bacillus subtilis wild-type membrane-bound enzyme were determined as +80, -240, and -25 mv, respectively. magnetic spin interactions between clusters s-1 and s-2 and between s-1 and s-3 were detected by using epr spectroscop ... | 1986 | 3021212 |
| bacillus subtilis mutant succinate dehydrogenase lacking covalently bound flavin: identification of the primary defect and studies on the iron-sulfur clusters in mutated and wild-type enzyme. | succinate dehydrogenase consists of two protein subunits and contains one fad and three iron-sulfur clusters. the flavin is covalently bound to a histidine in the larger, fp, subunit. the reduction oxidation midpoint potentials of the clusters designated s-1, s-2, and s-3 in bacillus subtilis wild-type membrane-bound enzyme were determined as +80, -240, and -25 mv, respectively. magnetic spin interactions between clusters s-1 and s-2 and between s-1 and s-3 were detected by using epr spectroscop ... | 1986 | 3021212 |
| [epidemiological and therapeutic studies of gonorrheal infection--clinical efficacy of sultamicillin]. | we conducted an epidemiological study including analyses of background factors of 192 male and 13 female patients with gonorrheal infection in the sapporo area and at the same time, investigated the therapeutic efficacy of sultamicillin, an ester linked prodrug of ampicillin and beta-lactamase inhibitor sulbactam in the treatment of these patients. the percentage of infections in sapporo was rather high in the young generation, being as high as 13.5% in teen-age boys and 30.8% in teen-age girls, ... | 1986 | 3008539 |
| multiple liver abscesses associated with bacteremia due to leuconostoc lactis. | leuconostoc species, which are members of the family streptococcacae, have only recently been recognized as potential pathogens. we describe a patient with type ii diabetes mellitus who had multiple liver abscesses associated with bacteremia due to leuconostoc lactis. to our knowledge, this is the first case of this association to be reported in the literature. | 2002 | 12477331 |
| leuconostoc lactis beta-galactosidase is encoded by two overlapping genes. | a 16-kb bamhi fragment of the lactose plasmid pnz63 from leuconostoc lactis nz6009 was cloned in escherichia coli mc1061 by using pacyc184 and was found to express a functional beta-galactosidase. deletion and complementation analysis showed that the coding region for beta-galactosidase was located on a 5.8-kb sali-bamhi fragment. nucleotide sequence analysis demonstrated that this fragment contained two partially overlapping genes, lacl (1,878 bp) and lacm (963 bp), that could encode proteins w ... | 1992 | 1624440 |
| complete nucleotide sequence and structural organization of ppb1, a small lactobacillus plantarum cryptic plasmid that originated by modular exchange. | a small cryptic plasmid designated ppb1 was isolated from lactobacillus plantarum bifi-38 and its complete 2899 bp nucleotide sequence was determined. sequence analysis revealed four putative open reading frames. based on sequence analysis two modules could be identified. first, the replication module consisted of a sequence coding for a replication protein (repb) and its corresponding target site, and two putative repressor proteins (repa and repc). sequence analysis indicated the possible synt ... | 2004 | 15518876 |
| mutations affecting regulation of the anabolic argf and the catabolic aru genes in pseudomonas aeruginosa pao. | the nucleotide sequence required for a fully functional promoter and operator of the pseudomonas aeruginosa argf gene (argfpo), the arginine-repressible gene for anabolic ornithine carbamoyltransferase, was defined within a 160 bp region. the streptomycin (sm) resistance genes strab of plasmid rsf1010 were fused to argfpo. this construct in p. aeruginosa strain pao conferred resistance to sm. mutants of strain pao were selected which were resistant to sm in the presence of arginine due to consti ... | 1992 | 1538697 |
| current approaches to the management of fungal infections in cancer patients: part 1. | fungal infections in the immunocompromised cancer patient may threaten survival and represent a major therapeutic challenge. granulocytopenia, especially, increases the risk of infection with the two most common fungal species, candida and aspergillus. in cancer patients, mucocutaneous disruptions due to administration of chemotherapeutic agents and use of iv lines, hickman catheters, and nasogastric tubes provide portals of entry for candida species. although cryptococcosis, histoplasmosis, and ... | 1992 | 1533782 |
| intracellular ph and the role of d-lactate dehydrogenase in the production of metabolic end products by leuconostoc lactis. | the kinetics of lactate dehydrogenase from leuconostoc lactis ncw1 were studied. the ph optimum for the enzyme depended on the concentration of pyruvate used in the assay and the enzyme displayed an ordered mechanism with respect to substrate binding. the km for pyruvate and nadh and the vmax of the enzyme decreased 20-, 30- and 6-fold respectively as the ph decreased from 8.0 to 5.0. no activators were found and none of the intermediates of the phosphoketolase pathway tested inhibited the enzym ... | 1992 | 1401357 |
| hepatitis b virus markers in 97 long-term hemodialysis patients. | a study was undertaken to assess the state of hepatitis b virus (hbv) infection in hemodialysis patients. from 97 hemodialysis patients tested, 51 were found to have at least one hepatitis b virus specific marker. 18 were hbsag carriers, 12 of these carriers have to be regarded as infectious as judged from the presence of hbeag and/or hbv-specific dna polymerase activity in the serum. antinuclear antibodies (ana) were found in the sera of approximately 20% of the hemodialysis patients with a hig ... | 1979 | 492411 |
| satellite association in human metaphases. a comparative study of normal individuals, patients with down syndrome and their parents. | | 1979 | 154489 |
| spasticity: actions of selectivity activated group ii muscle spindle afferents on extensor motoneurons. | | 1975 | 129880 |
| studies on the large subunit rrna genes and their flanking regions of leuconostocs. | the 16s-23s (spacer-1) and 23s-5s (spacer-2) rrna intergenic spacer regions of leuconostoc lactis, leuconostoc mesenteroides, leuconostoc mesenteroides subsp. dextranicum, and leuconostoc mesenteroides subsp. cremoris were amplified by polymerase chain reactions and sequenced. the 23s rrna genes of leuconostoc lactis, leuconostoc mesenteroides, and leuconostoc mesenteroides subsp. dextranicum were also sequenced. the rnase iii-like and rnase e processing sites, as well as putative antiterminatio ... | 1998 | 9851024 |
| controlled gene expression systems for lactic acid bacteria: transferable nisin-inducible expression cassettes for lactococcus, leuconostoc, and lactobacillus spp. | a transferable dual-plasmid inducible gene expression system for use in lactic acid bacteria that is based on the autoregulatory properties of the antimicrobial peptide nisin produced by lactococcus lactis was developed. introduction of the two plasmids allowed nisin-inducible gene expression in lactococcus lactis mg1363, leuconostoc lactis nz6091, and lactobacillus helveticus cnrz32. typically, the beta-glucuronidase activity (used as a reporter in this study) remained below the detection limit ... | 1997 | 9361443 |
| a leuconostoc lactis protein with homology to ribosomal protein s1 shares common epitopes and common dna binding properties with a mammalian dna binding nuclear factor. | a mouse testis cdna expression library (clontech) was screened with a synthetic oligonucleotide ligand containing ct-rich motifs derived from the rat skeletal muscle actin gene promoter. these motifs bind nuclear proteins, and seem to be involved in the regulation of the gene. analysis of isolated clones, which expressed proteins that specifically bind the oligonucleotide, indicated that they were derived from a single gene. this gene was identified as a contaminant of bacterial origin (leuconos ... | 1997 | 9034319 |
| two genes encoding the beta-galactosidase of lactobacillus sake. | the beta-galactosidase of lactobacillus sake dsm 20017 is encoded by two genes located on its chromosome. these genes designated lacl and lacm were cloned in escherichia coli nm 554 on an 8.65 kbp hindiii fragment inserted in vector prb473. deletion analysis of the originally cloned fragment revealed that both genes are required for the formation of a functional beta-galactosidase. lacl and lacm are transcribed as a single transcript of approximately 2.9 kbp starting 34 bp upstream of the transl ... | 1995 | 8574399 |
| leuconostoc holzapfelii sp. nov., isolated from ethiopian coffee fermentation and assessment of sequence analysis of housekeeping genes for delineation of leuconostoc species. | a gram-positive, ovoid lactic acid bacterium, strain lmg 23990(t), was isolated from ethiopian coffee fermentation. 16s rrna gene sequence analysis indicated that the novel strain belongs to the genus leuconostoc, with leuconostoc citreum and leuconostoc lactis as the closest neighbours (99.6 and 99.0 % 16s rrna gene sequence similarity, respectively). genotypic fingerprinting by fluorescent amplified fragment length polymorphism, whole-cell protein electrophoresis, dna-dna hybridizations, compa ... | 2007 | 18048756 |
| development and use of a selective medium for isolation of leuconostoc spp. from vegetables and dairy products. | a selective medium (lusm medium) for the isolation of leuconostoc spp. was developed. this medium contained 1.0% glucose, 1.0% bacto peptone (difco), 0.5% yeast extract (bbl), 0.5% meat extract (difco), 0.25% gelatin (difco), 0.5% calcium lactate, 0.05% sorbic acid, 75 ppm of sodium azide (sigma), 0.25% sodium acetate, 0.1% (vol/vol) tween 80, 15% tomato juice, 30 micrograms of vancomycin (sigma) per ml, 0.20 microgram of tetracycline (serva) per ml, 0.5 mg of cysteine hydrochloride per ml, and ... | 1993 | 8434926 |
| nucleotide sequence and structural organization of the small, broad-host-range plasmid pci411 from leuconostoc lactis 533. | the nucleotide sequence of the leuconostoc lactis 533 cryptic plasmid pci411 (2926 bp) was determined. analysis revealed the presence of three open reading frames (orfs). orf 1 was capable of encoding a 24.9 kda peptide which shared homology with the replication initiation protein (repb) from a number of gram-positive rolling circle plasmids. orf 2 could encode a peptide of 6.6 kda which was homologous to the repc protein of the lactococcal plasmid pwv01. a function could not be assigned to orf ... | 1994 | 7952178 |
| polyphasic study of microbial communities of two spanish farmhouse goats' milk cheeses from sierra de aracena. | the microbial communities present in 2 different types of farmhouse goats' milk cheese from the aracena mountains (southwest spain), quesailla arochena (hard cheese) and torta arochena (soft cheese), have been studied using both culture-dependent and culture-independent techniques. all bacterial isolates were clustered by using randomly amplified polymorphic dna (rapd) and identified by 16s rrna gene sequencing, species-specific pcr and multiplex pcr. thus a total of 26 different species were id ... | 2009 | 19269572 |
| characterization of plasmid-encoded citrate permease (citp) genes from leuconostoc species reveals high sequence conservation with the lactococcus lactis citp gene. | the citrate permease determinant (citp) in several leuconostoc strains was demonstrated to be plasmid encoded by curing experiments and hybridization studies with a dna fragment containing the citp gene from lactococcus lactis subsp. lactis biovar diacetylactis ncdo176. cloning and nucleotide sequence analysis of leuconostoc lactis nz6070 citp revealed almost complete identity to lactococcal citp. | 1995 | 7487049 |
| the effects of vasectomy on the testis. | the increased use of vasectomy for population control has led to a large number of animal studies on the morphologic, physiologic, and immunologic responses to this procedure. the extent, nature, and timing of morphologic alterations in the testis showed marked species variations and ranged from autoimmune orchitis to an absence of detectable changes. physiologic studies indicated, contrary to expectation, that the hydrostatic pressure was elevated only in the distal part of the epididymis and ... | 1985 | 4058509 |
| influence of lactose-citrate co-metabolism on the differences of growth and energetics in leuconostoc lactis, leuconostoc mesenteroides ssp. mesenteroides and leuconostoc mesenteroides ssp. cremoris | the biodiversity of growth and energetics in leuconostoc sp. has been studied in mrs lactose medium with and without citrate. on lactose alone, ln. lactis has a growth rate double that of ln. cremoris and ln. mesenteroides. the ph is a more critical parameter for ln. mesenteroides than for ln. lactis or ln. cremoris; without ph control ln. mesenteroides is unable to acidify the medium under ph 4.5, while with ph control and as a consequence of a high y(atp) its growth is greater than ln. lactis ... | 1999 | 10794137 |
| molecular diversity of leuconostoc mesenteroides and leuconostoc citreum isolated from traditional french cheeses as revealed by rapd fingerprinting, 16s rdna sequencing and 16s rdna fragment amplification. | for a long time, the identification of the leuconostoc species has been limited by a lack of accurate biochemical and physiological tests. here, we use a combination of rapd, 16s rdna sequencing, and 16s rdna fragment amplification with specific primers to classify different leuconostocs at the species and strain level. we analysed the molecular diversity of a collection of 221 strains mainly isolated from traditional french cheeses. the majority of the strains were classified as leuconostoc mes ... | 2000 | 10930080 |
| genome sequence of leuconostoc argentinum kctc 3773. | leuconostoc argentinum is one of the most prevalent lactic acid bacteria present during the manufacturing process of kimchi, the best-known traditional korean dish. here, we present the draft genome sequence of type strain kctc 3773 of leuconostoc argentinum (1,720,683 bp, with a g+c content of 42.9%), which consists of 98 large contigs (>100 bp in size). | 2010 | 20952569 |
| a critical review of the literature on ratings scales for perceived exertion. | the study of human performance and perceived exertion during physical activity has been an area of considerable interest and research for over 50 years. this review considers the evidence of many investigators who have been researching the physiological basis as well as non-physiological basis for the ratings of perceived exertion. during low levels of activity, physical perception in the working muscles appears to be the primary stimulus for effort perception. when work intensity exceeds the la ... | 1985 | 3892628 |
| natural populations of lactic acid bacteria associated with silage fermentation as determined by phenotype, 16s ribosomal rna and reca gene analysis. | one hundred and fifty-six strains isolated from corn (zea mays l.), forage paddy rice (oryza sativa l.), sorghum (sorghum bicolor l.) and alfalfa (medicago sativa l.) silages prepared on dairy farms were screened, of which 110 isolates were considered to be lactic acid bacteria (lab) according to their gram-positive and catalase-negative characteristics and, mainly, the lactic acid metabolic products. these isolates were divided into eight groups (a-h) based on the following properties: morpholo ... | 2011 | 21282025 |
| nucleotide sequence, expression and transcriptional analysis of the bifidobacterium longum mb 219 lacz gene. | the gene encoding beta-galactosidase was isolated by functional complementation of escherichia coli from bifidobacterium longum mb219, which exhibited the highest activity among ten bifidobacterium strains tested of the species b. longum, b. breve, b. adolescentis, b. indicum, b. animalis and b. cuniculi. the nucleotide sequence of the 5.0-kb fragment conferring the positive beta-galactosidase phenotype to e. coli revealed the presence of a lacz-type gene encoding a 1023-amino-acid protein that ... | 2000 | 10985745 |
| multiplex pcr-based detection and identification of leuconostoc species. | a multiplex polymerase chain reaction (pcr) assay has been developed for rapid and reliable identification of leuconostoc species, by using species-specific primers targeted to the genes encoding 16s rrna. this assay can detect and differentiate leuconostoc species from mixed populations in natural sources as well as from pure cultures, within 3 h. this assay system consists of a total of 10 primers, two primers from each target species, and comprises two multiplex pcr reactions: one reaction fo ... | 2000 | 11111031 |
| cross-reaction of lupus anti-dsdna antibodies with protein translation factor ef-2. | this report elucidates a new cross-reactive intracellular target of anti-dsdna antibodies. previous experiments have demonstrated that some anti-dsdna antibodies penetrate cells grown in tissue culture and all inhibit in vitro translation. data implicate a cross-reactive antigen directly involved in protein synthesis: elongation factor-2 (ef-2). ef-2 was identified by n-terminal sequencing of a band identified with an antibody to the ribosomal protein s1 from leuconostoc lactis in western blot a ... | 2001 | 11161987 |
| gut hormones in fetal distress. | | 1999 | 91067 |
| [relation between endocrinology and psychiatry. some recent developments]. | relations between endocrinology and psychiatry are developing in two main directions: behavioural effects of hormones. after the effects of peripheral hormones, recent works are concerned with a.c.t.h. and antidiuretic hormone (which interfere with memory process) and with hypothalamic peptides (which might have psychotropic properties, independant of their endocrine regulation function). -correlations between psychiatric syndroms and endocrine disorders. in some mentally depressed patients, dis ... | 2003 | 165466 |
| pacing-induced alternate wenckebach periods: incidence and clinical significance. | alternate wenckebach periods have been defined as episodes of 2:1 atrioventricular (av) block in which conducted p waves exhibit progressive pr prolongation until two or three successively blocked p waves. ocurrence of this phenomenon during atrial pacing has been established. thirty-six patients were studied and right atrial pacing was achieved at increasing rates up to 350 beats/min in order to induce alternate wenckebach periods. his bundle recordings were obtained in every patient. the patie ... | 2001 | 95224 |
| evaluation of predictive formulae for determining metabolic rate during cold water immersion. | five models predicting shivering thermogenesis on the basis of steady state skin and core temperature were evaluated: hayward et al., stolwijk and hardy,; nadel et al.,; timbal et al., and brown and brengelmann, using the empirical data derived from a cold water immersion study by morrison et al. a residual analysis indicated that all models generated substantial errors of prediction. the best overall predictors were expressions suggested by hayward et al., while the predictive equation of nadel ... | 2013 | 3741291 |
| purification and characterisation of acetolactate decarboxylase from leuconostoc lactis ncw1. | a two-step strategy involving deae-cellulose and poros pi anion exchange chromatography has been developed for rapid purification of acetolactate decarboxylase (ald) from leuconostoc lactis ncw1. this results in 5333-fold purification with a yield of 30%. purified ald is a dimer of 49-kda subunits, has a ph optimum of 6.0, a pi of 4.2 and its activity is independent of metals or branched chain amino acids. at the optimum ph, the k(m) for 2-acetolactate (ala) was found to be 1.3 mm and the turnov ... | 2001 | 11164316 |
| measurement of the alpha 2 component of a novel oncodevelopmental antigen by a rapid electroimmunoassay. | a method was required for the quantification of a novel oncodevelopmental antigen which increases in the serum of pregnant women as well as patients with ovarian cancer. the native antigen is composed of two components having alpha 2 and beta electrophoretic mobilities. an assay method, involving electroimmunodiffusion according to laurell, was developed for the measurement of the alpha 2 component of the antigen. the method is sufficiently sensitive to determine significant differences in the l ... | 2013 | 3668256 |
| new horizons in drug delivery. | | 1998 | 106934 |
| the microbiology of south african traditional fermented milks. | a total of 15 samples of traditional fermented milk were collected from individual households in south africa and namibia. lactic acid bacteria dominated the microflora of these samples, especially the genera leuconostoc, lactococcus and lactobacillus. other groups identified included pyogenic streptococci and enterococci. the dominant lactococci species was lactococcus lactis subsp. lactis. eighty-three percent of the leuconostoc isolates were identified as leuconostoc mesenteroides subsp. dext ... | 2001 | 11246902 |
| electronic activation of the spincters in the treatment of rectal prolapse. | | 2005 | 1081232 |
| isolation, identification and changes in the composition of lactic acid bacteria during the malting of two different barley cultivars. | malt has a complex microbial population, which changes as the malting process commences. little is known about the proliferation of lactic acid bacteria (lab) in each of the malting phases. in this study, we determined the number of lab present in the different phases of malting with clipper and prisma barley cultivars. the strains were identified to species level by using numerical analysis of total soluble cell protein patterns, rapd-pcr banding patterns and 16s rrna sequencing. the number of ... | 2002 | 12038579 |
| a rapid method for identification of typical leuconostoc species by 16s rdna pcr-rflp analysis. | a polymerase chain reaction (pcr)-restriction fragment length polymorphism (rflp) method was developed to detect and identify typical leuconostoc species. this method utilises a set of specific primers for amplification of the 16s rdna region of typical leuconostoc species. all leuconostoc-type strains, all leuconostoc isolates from kimchi, korea's traditional, fermented vegetable product, and strains from closely related genera were examined to verify the identification by this method. the prim ... | 2003 | 14500021 |
| radioassay of serum folate: some criticisms. | | 2015 | 4683990 |
| [extrapleural surgical correction of congenital esophageal atresia]. | | 2016 | 4845554 |
| a case of osteomyelitis due to leuconostoc lactis. | a baby case of haematogenous rib osteomyelitis that was caused by leuconostoc lactis was presented. the patient had high fever and an abscess formation on the right scapula. diagnosis was made with the results of blood, bone and abscess cultures, pathological findings of the involved rib and direct bone graphies. the patient was treated succesfully with cefotaxime for 6 weeks. | 2007 | 17366068 |
| development of innovative pediocin pa-1 by dna shuffling among class iia bacteriocins. | pediocin pa-1 is a member of the class iia bacteriocins, which show antimicrobial effects against lactic acid bacteria. to develop an improved version of pediocin pa-1, reciprocal chimeras between pediocin pa-1 and enterocin a, another class iia bacteriocin, were constructed. chimera ep, which consisted of the c-terminal half of pediocin pa-1 fused to the n-terminal half of enterocin a, showed increased activity against a strain of leuconostoc lactis isolated from a sour-spoiled dairy product. t ... | 2007 | 17601819 |
| coronal axis symmetry in basal and squamous cell carcinomas. | | 2006 | 1552073 |
| dna fingerprinting of lactic acid bacteria in sauerkraut fermentations. | previous studies using traditional biochemical identification methods to study the ecology of commercial sauerkraut fermentations revealed that four species of lactic acid bacteria, leuconostoc mesenteroides, lactobacillus plantarum, pediococcus pentosaceus, and lactobacillus brevis, were the primary microorganisms in these fermentations. in this study, 686 isolates were collected from four commercial fermentations and analyzed by dna fingerprinting. the results indicate that the species of lact ... | 2007 | 17921264 |
| analysis of vaginal lactic acid producing bacteria in healthy women. | vaginal lactic acid-producing bacteria of 80 pre-menopausal women were studied by isolation on blood and deman-rogosa-sharpe agar, pcr with group-specific primers for lactobacillus-denaturing gradient gel electrophoresis (dgge), and pcr with specific primers for v3 region in 16s rrna-temporal temperature gel electrophoresis (ttge). conventional isolation method on media detected only one lactobacillus (lactobacillus brevis) while ttge detected only lactobacillus sp. dgge detected seven lactobaci ... | 2007 | 18176534 |
| study of microbial diversity in raw milk and fresh curd used for fontina cheese production by culture-independent methods. | the bacterial populations of raw milk employed for the production of fontina cheese in alpine farms located in different valleys and altitudes (from 700 to 2246 m above sea level) were investigated by culture independent techniques. total microbial dna was isolated from milk and curd samples and used as template in polymerase chain reaction (pcr) to study the hypervariable v3 region of the bacterial 16s rrna gene and analyzed by denaturing gradient gel electrophoresis (dgge). representative band ... | 2009 | 19232767 |
| kinetics of unfolding and refolding of proteins. 3. results for lysozyme. | | 2015 | 4347737 |
| leuconostoc palmae sp. nov., a novel lactic acid bacterium isolated from palm wine. | a novel lactic acid bacterium, strain tmw 2.694(t), was isolated among other lactic acid bacteria from palm wine, an alcoholic beverage produced from the sap of various palm tree species. strain tmw 2.694(t) is a gram-positive, facultatively anaerobic, catalase-negative, non-spore-forming coccus, occurring in long chains. phylogenetic analysis based on 16s rrna gene sequencing positioned strain tmw 2.694(t) in a distinct line of descent within the genus leuconostoc, with the closest neighbours b ... | 2009 | 19406772 |
| [the effect of estrogens and progesterone on the membrane potential of the myometrium]. | | 2015 | 4651076 |
| changes in the microbial composition of raw milk induced by thermization treatments applied prior to traditional greek hard cheese processing. | the microbiological quality, safety, and composition of mixtures of ewe's and goat's milk (90:10) used for cheesemaking were evaluated before and after thermization at 60 and 67 degrees c for 30 s. such mild thermal treatments are commonly applied to reduce natural contaminants of raw milk before processing for traditional hard greek cheeses. raw milk samples had an average total bacterial count of 7.3 log cfu/ml; most of these bacteria were lactic acid bacteria (lab) and pseudomonads. the lab f ... | 2009 | 19435227 |
| purification and characterization of a novel glucansucrase from leuconostoc lactis eg001. | a gene encoding glucansucrase was identified in leuconostoc lactis eg001 isolated from lactic acid bacteria (lab) in kimchi, a traditional korean fermented food. the l. lactis eg001 glucansucrase gene consists of 4503 bp open reading frame (orf) and encodes an enzyme of 1500 amino acids with an apparent molecular mass of 165 kda. the deduced amino-acid sequence showed the highest amino-acid sequence identity (75%) to that of dextransucrase of l. mesenteroides. the gene was cloned and over-expres ... | 2010 | 19853426 |