| purification and properties of the extracellular dextransucrase from leuconostoc mesenteroides nrrl b-1299. | dextransucrase ec 2.4.1.5 activity from cell-free culture supernatant of leuconostoc mesenteroides nrrl b-1299 was purified by (nh4)2so4 fractionation, adsorption on hydroxyapatite, chromatography on deae-cellulose and gel filtration on sephadex g-75. the extracellular enzyme was separated into two principal forms, enzymes i and n, and the latter was shown to be an aggregated form of the protomer, enzyme i. enzymes i and n were both electrophoretically homogeneous and their relative activities r ... | 1976 | 8441 |
| glucose-6-phosphate dehydrogenase from leuconostoc mesenteroides. interaction of the enzyme with coenzymes and coenzyme analogs. | | 1976 | 11751 |
| identification of essential arginine residues in glucose-6-phosphate dehydrogenase from leuconostoc mesenteroides. | | 1977 | 16915 |
| purification and characterization of human antiplasmin, the fast-acting plasmin inhibitor in plasma. | | 1977 | 21075 |
| effect of intraventricular injections of 6-hydroxydopamine in neonatal rats on the catecholamine levels and tyrosine hydroxylase activity in brain regions at maturity. | | 1977 | 22332 |
| simultaneous analysis of nad- and nadp-linked activities of dual nucleotide-specific dehydrogenases. application to leuconostoc mesenteroides glucose-6-phosphate dehydrogenase. | a method is described which enables one to assay simultaneously the nad- and nadp-linked reactions of dehydrogenases which can utilize both coenzymes. the method is based on the fact that the thionicotinamide analogs of nadh and nadph absorb light maximally at 400 nm, a wavelength sufficiently far removed from the absorbance maximum of nadh and nadph to permit measurements of the simultaneous reduction of nad+ (or nadp+) and the thionicotinamide analog of nadp+ (or nad+). application of the meth ... | 1979 | 35541 |
| hemodynamics of hyperthyroidism. the effects of autonomic nervous blocking and anti-thyroid drug treatment. | this work was intended to analyze the sympathetic and parasympathetic factors affecting the hemodynamics of hyperthyroidism. seven patients with hyperthyroidism, diagnosed based on the determinations of bmr, 131i-uptake, t3-resin sponge uptake (t3-rsu), and serum level of thyroxine (t4) were subjected to the study. the hemo-dynamic estimation was done (1) at rest, (2) after vagus blocking by the injection of 0.04 mg/kg b.wt. atropine and 0.2 mg/kg b.wt. propranolol before (hyperthyroid state=h) ... | 1976 | 59000 |
| enzymic polyadenylation of 5s ribosomal ribonucleic acid and synthesis of a complementary deoxyribonucleic acid. | the 5s ribosomal rna has been isolated, pure and intact, from rat liver (5 mg of 5s rna from 150g of liver). the 5s rna serves as a primer for calf thymus poly(a) polymerase with 20% of the efficiency of (ap)3a. bacterial 5s rna and transfer rna also serve as primers; rat liver 18s and 28s ribosomal rnas support poly(a) synthesis poorly. neither the 5s rna primer nor the appended poly(a) tract is nicked or degraded by poly(a) polymerase, and initiation of poly(a) tracts on 5s rna primers continu ... | 1979 | 88957 |
| the pathophysiology of asthma. | because postmortem studies of humans provide little information on the initial pathophysiologic events in asthma, animal models have been developed. recently the ascaris-allergic rhesus monkey has provided an opportunity to examine the onset of pathophysiologic changes following challenge and to correlate them with airway structure. these studies have suggested that the initial interaction between antigen and mast cells may occur in the bronchial lumen or in the epithelium superficial to the tig ... | 1979 | 89006 |
| dopamine disorder in duodenal ulceration. | cysteamine-induced duodenal ulcers in rats were prevented by the dopamine agonists bromocriptine, lergotrile, and apomorphine, whereas both the severity of duodenal ulcers and the mortality among cysteamine-treated rats were raised by the dopamine receptor antagonist, haloperiodol. bromocriptine and lergotrile greatly reduced gastric-acid output in cysteamine-treated rats. a review of the literature shows a high incidence of duodenal ulcers in patients with parkinson's disease (associated with d ... | 1979 | 90970 |
| presence of fibronectin in basement membranes and acidic structural glycoproteins from human placenta and lung. | cold-insoluble globulin was detected in both trophoblast and alveolar basement membrane preparations, whereas it was not detected in gbm preparations. acidic structural glycoproteins from both placental villi and lung parenchyma, which were extracted with 0.3 m acetic acid and recovered by adjusting the ph to 4.7, also contained cig. fractions of tbm, solubilized by either dilute alkali (0.01 n naoh), by reduction and alkylation of the disulfide bonds, or by 0.3 m acetic acid extraction, all con ... | 1978 | 91337 |
| estrogen binding in the neonatal neocortex. | | 1979 | 91415 |
| applications of biologic tumor markers in testicular cancer. | quantitative measurement of serum alphafetoprotein (afp) and human chorionic gonadotropin (hcg) by double antibody radioimmunoassays originally developed at the national cancer institute, bethesda, md) has been performed in 130 patients with seminoma and in 300 patients with nonseminomatous testicular cancer. ten of 130 (7.7%) patients with seminoma, 99 of 152 (65%) patients with embryonal carcinoma with or without teratoma, and five of five (100%) patients with pure choriocarcinoma had an eleva ... | 1979 | 91433 |
| amplification of plaque-forming cells in the spleen after intracloacal antigen stimulation in neonatal chicken. | neonatal chickens were primed by multiple or a single injection with sheep red blood cells (srbc) into the cloacal lumen and then were challenged by intravenous injection with the same antigen. direct plaque-forming cells (pfc) in the spleen were markedly increased shortly after intracloacal priming. this enhancing effect was antigen specific and was abolished by surgical bursectomy 24 h after intracloacal priming. a similar effect was also found in mature adult chickens, although the degree of ... | 1979 | 91577 |
| sensitivity of the short-range spinal interneurons of the cat to experimental spinal cord trauma. | the technique of retrograde axoplasmic transport was used to demonstrate the effect of experimental spinal cord injury on the spinal interneurons in the upper lumbar and lower thoracic segments of cats. force of varied intensity was applied to the dorsal surface of the spinal cord and horseradish peroxidase (hrp) was injected into the next caudal segment. a large impact (250 to 350 gm-cm) inducing permanent paraplegia of the hind legs blocked the axoplasmic transport instantaneously in both cran ... | 1979 | 91677 |
| association between pre-transplant natural kill and graft-versus-host disease after stem-cell transplantation. | natural killer activity against herpes simplex virus type 1 infected fibroblasts nk(hsv-1) was studied prospectively in patients undergoing allogenic bone-marrow or fetal-tissue stem-cell transplantation. thirteen patients showed evidence of engraftment and survived long enough to develop graft-versus-host disease (gvhd). of this group, all of the seven having normal nk(hsv-1) activity before transplantation acquired gvhd and the six having low nk(hsv-1) had no evidence of gvhd. these results we ... | 1979 | 91840 |
| human lung tissue and anaphylaxis:cyclic nucleotide response and prostaglandin synthesis. | | 1979 | 91946 |
| [heterologous transplantation of the human medulloblastoma--with particular reference to the histogenesis of medulloblastoma (author's transl)]. | | 1979 | 91984 |
| [production of antiserum to neurotoxin-2 from naja naja oxiana cobra venom and reaction with the toxin]. | antiserum to neurotoxin-2 from the venom of the cobra was obtained by immunization of rabbits via the injection of the toxin into the lymphatic nodes and by two re-immunizations via the injection of the toxin into the blood and intramuscularly. using scores of micrograms of the toxin, specific antisera with high antibody titer were obtained. in spite of high specificity of separate stages of the reactions, using indirect immune fluorescence technique, no specific fluorescence was found in the en ... | 1979 | 92118 |
| [hydrolysis of glucose 6-phosphate in bovine thyroid tissue (proceedings)]. | | 1979 | 92262 |
| tracing neural connections of human brain with selective silver impregnation. observations on geniculocalcarine, spinothalamic, and entorhinal pathways. | in selected human brains, it is possible to study the efferent connections of a damaged site with the suppressive silver impregnation techniques described by nauta and gygax. autopsy specimens with circumscribed lesions of recent origin ( one to five weeks before death) are suitable. however, the large size of the human brain and the lack of perfusion with fixative necessitates modifications in the methodology used on experimental animals. with these modifications, it has been possible to trace ... | 1979 | 92302 |
| synthesis of an immunogen with catechol estrogen as immunodeterminant group and its characterization by the induced antibodies. | | 1979 | 92446 |
| characterization of a 130,000-dalton glycoprotein isolated from pulmonary secretions of patients with alveolar proteinosis. | a new glycoprotein with an apparent molecular weight of 130,000 was isolated, purified, and partially characterized from the pulmonary secretions which accumulate so massively in the lungs of patients suffering from pulmonary alveolar proteinosis. the amino acid analysis of the glycoprotein showed the presence of relatively high amounts of glycine, glutamic acid, aspartic acid, leucine, and valine, and small amounts of hydroxyproline, but no hydroxylysine. it contains approximately 6% hexose, 3% ... | 1979 | 92460 |
| [lipopolysaccharide study of the cell membranes of bacteria in the genus pseudomonas. v. the serological characteristics]. | | 1979 | 92749 |
| changes of basic biochemical indices in rat liver tissue after intraperitoneal application of endotoxin. | | 1979 | 92874 |
| genetics of formamidase-5 (brain formamidase) in the mouse: localization of the structural gene on chromosome 14. | a single formamidase, which is different from the formamidases found in other tissues occurs in the brains of mice. this enzyme is here called formamidase-5 and the gene symbol is designated for-5. two alleles are recognized on the basis of their differential heat sensitivity:for-5b is relatively heat stable and is present in strain c57bl/6j, while for-5d is relatively heat sensitive and is present in strain dba/2j. the heat sensitivity of formamidase-5 in 44 other inbred strains and substrains ... | 1979 | 92985 |
| testing the properties of an experimental batch of zoster immune gammaglobulin (zig). | the methods of indirct haemagglutination (ih) and precipitation in gel (id) were employed to test the level of varicella-zoster (vz) antibodies in an experimental batch of zoster gammaglobulin (zig). the titre of indirect haemagglutinating antibodies in zig was about 64 times higher than in the ordinary batches of normal immunoglobulin and about 8 times higher in comparison with the level of the initial plasma pool. in the reaction of precipitation in gel, zig produced 5 to 6 zones. in compariso ... | 1979 | 93127 |
| rapid removal of acid from glycol methacrylate for improved histological embedding. | a method for the rapid and complete removal of methacrylic acid from commercial samples of glycol methacrylate is presented. it entails conversion of the acid to an insoluble n-acylurea by treatment with an equivalent amount of n,n'-dicyclohexylcarbodiimide. sections of tissue embedded in polymer prepared from the purified monomer can be stained with basic dyes without simultaneously staining the polymer. | 1979 | 93324 |
| [antitumor resistance induced by immunization with normal definitive tissues]. | immunization of c3ha mice with homogenates of various normal definitive tissues, obtained from syngeneic, allogeneic and xenogeneic donors, resulted in an increase of antitumor resistance, as evidenced by a considerable retardation of growth of the transplantable strain-specific hepatoma 22a. the effect may be due to sensibilization of animals to the antigens of normal definitive cells, and, in particular to the tissue-specific antigens characteristic of cells of the tumor in question. | 1979 | 93333 |
| argentophil neuronal perikarya and neurofibrils induced by postmortem trauma and hypertonic perfusates. | argentophil neuronal perikarya and perikaryal neurofibrils similar to those illustrated in ramón y cajal's classical studies have in the present investigation been found to be manifestations of the chromophil neuron. conclusive evidence of such association was obtained by silver impregnation with the bodian technique of sections previously stained with cresyl violet. regardless of the fixative used, silver-impregnated neurofibrils were evident when (1) normal tissues were fixed by immersion or u ... | 1979 | 93388 |
| comparison of auramine-rhodamine b and acridine orange for staining of acid-fast bacteria. | in cooperation of 6 laboratories in czechoslovakia and in the gdr, the efficiency of auramine-rhodamine b (ar) and acridine orange (ao) (short-time method) for staining of acid-fast bacilli was compared. whereas a former comparison of ar and ao (original method) pointed out the superiority of ar, the investigation of both methods used as short-time procedures showed significantly more acid-fast rods after using ao. the number of "false positive" results was somewhat higher on ar staining. howeve ... | 1979 | 93610 |
| calcium and cyclic nucleotide interaction in secretion of amylase from rat pancreas in vitro. | 1. interaction of ca2+ and cyclic nucleotides in stimulus-secretion coupling in rat pancreas in vitro was studied utilizing the divalent cation inophore a23187. phosphodiesterase inhibitors, cyclic nucleotides and cholera toxin. 2. amylase secretion was increased by the ionophore in the presence of extracellular ca2+ in a dose-dependent fashion. activation of cck-pz receptors simultaneously with induction of amylase secretion by a23187 did not alter amylase secretion whereas theophylline or caff ... | 1979 | 93637 |
| determining the polarity of the map of crosslinked interactions in escherichia coli 16 s ribosomal rna. | | 1979 | 93647 |
| cytologic investigations in chlamydia infection. | chlamydia is an extremely common genital infection. it can cause serious disseminated disease and can infect the neonate and the sexual partner. morphologically, chlamydia infection was identified in routine fast smears obtained from 160 women. chlamydia infection manifests as intracytoplasmic coccoid and inclusion bodies in the metaplastic cells. infected cells also reveal cytomegaly and multinucleation. immunofluorescence studies, electron microscopy, tissue studies, histochemical techniques a ... | 1979 | 93839 |
| mechanism of action of beta-d-xylosidase from bacillus pumilus prl b12: ph- and alpha-deuterium kinetic isotope effects [proceedings]. | | 1979 | 93945 |
| tissue distribution and elimination of digoxin and methyldigoxin after single and multiple doses in dogs. | glycoside concentrations in plasma and various tissues of dogs were determined after a single and repeated intravenous administration of tritium-labeled digoxin and beta-methyldigoxin. twenty-four hours after single dosing, the highest concentrations were measured in the kidney. this was followed by the heart, adrenal gland, liver, pancreas, lung, spleen, diaphragm, and skeletal muscle. the glycoside concentration in the brain was low after a single administration of dogoxin. a higher concentrat ... | 1979 | 94391 |
| [cross reactions of eight yeasts and their importance in serological candida diagnostic (author's transl)]. | while the antigen structure of yeast cell walls is well known, the immunological cross reactivity of these is often difficult to interpret. the cross reactions of eight yeasts were tested in rabbit hyperimmune sera by the indirect immunofluorescence test. among the eight species examined were the medically important yeasts c. albicans, c. pseudotropicalis, c. krusei, c. parapsilosis, c. tropicalis, t. glabrata, and c. guilliermondii and the apathogenic yeast s. cerevisiae. it has been shown, tha ... | 1979 | 94650 |
| effect of allopurinol on pyrimidine metabolism in human white blood cells. role of the salvage pathway. | | 1979 | 94760 |
| inhibition and stimulation of histamine release by oxatomide. | | 1979 | 95250 |
| the use of an oscillating-tube densitometer as a tool in enzyme kinetics. determination of the influence of sodium ascorbate on invertase, dextransucrase and dextranase. | the use of a commercial oscillating-tube densitometer with an accuracy of 4 . 10(-7) g/cm3 for the determination of enzyme-kinetics constants is tested. this method is applied to the investigation of the influence of vitamin c (sodium ascorbate) on the glycolytic enzymes invertase, dextransucrase and dextranase. invertase is inhibited uncompetitively, dextransucrase non-competitively. there is no significant effect of the vitamin on dextranase. the comparison of the mechanisms of the three enzym ... | 1979 | 95345 |
| [some aspects relating to the aflatoxin generation during the self-heating of cereals]. | in a stored batch of grain which was already affected by mould-formation tests were carried out with the known aflatoxin producer aspergillus flavus. a significantly lower aflatoxin production ensued if the mould growth was not connected with self-heating of the stored product. however, in conformity with increasing mould formation the germinating power was adversely affected and the significant signs (fatty acid number, reductive and none-reductive sugars) were influenced in the grain, irreleve ... | 1978 | 98933 |
| estimation of antibodies specific for dextran. | methods are described for the isolation and characterization of picogram quantities of anti-dextran antibodies. 14c-dextrans produced by using the dextransucrases of leuconostoc mesenteroides strains b1355 and b512 were used in a radioimmunoassay. the specificity of this assay was verified by using cell cytoplasmic lysates from mouse plasmacytomas, j558 (anti-alpha 1 leads to 3 dextran) and w3129 (anti-alpha 1 leads to 6 dextran). dextran produced by strain b1355 and insolubilized with epichloro ... | 1978 | 99480 |
| site dependence for topical absorption of nitroglycerin in rats. | | 1978 | 99511 |
| evidence for an essential dna component in the scrapie agent. | | 1978 | 99663 |
| [some ultrastructural characteristics of the sporal envelops of bacillus cereus]. | | 1977 | 99733 |
| aggression increase and water competition decrease in squirrel monkeys given physostigmine injections. | six adult squirrel monkeys (samiri sciureus) were tested in pairs for social dominance in a water competition task. dominance was defined by two methods: (1) number and direction of aggressive responses, and (2) successful water competition as assessed by latency to the water bottle, latency to accumulate 15 seconds of drinking, and total drinking duration. monkeys were assigned to pairs on a "round robin" basis so that each monkey was paired with all other monkeys under control, saline, and thr ... | 1978 | 99752 |
| [erythrocyte carbonic anhydrase isozymes in patients with iron deficiency anemia and their clinical significances (author's transl)]. | the levels of erythrocyte carbonic anhydrase isozymes (ca-b and ca-c) were determined by a quantitative immunochemical technique in order to elucidate their clinical significances in patients with iron deficiency anemia. the specific antibodies against rabbit ca-b and ca-c were also obtained, and the levels of rabbit erythrocyte carbonic anhydrase b and c isozymes were assayed immunochemically in experimental hemolytic and bleeding anemia. simultaneously carbonic anhydrase isozymes were synthesi ... | 1978 | 101434 |
| hemolytic streptococci in supramammary lymph nodes from healthy pigs from the peruvian jungle. | hemolytic streptococci were isolated from 53 percent (18) supramammary lymph nodes from healthy adult pigs raised at the peruvian jungle. the isolates were identified as streptococcus zooepidemicus, streptococcus infrequens, streptococcus faecalis and groups p and s. the possible significance of the findings are discussed. | 1978 | 101478 |
| the philadelphia chromosome in acute leukemia. | the cytogenetics, cytology and cytochemistry, clinical findings, therapeutic response and survival of patients presenting with acute leukemia and the philadelphia chromosome (ph1) are briefly reviewed based upon a survey of the world literature and 16 cases seen at the university of minnesota during the last 10 years. details regarding the 16 cases from the university of minnesota series are presented and two appendices listing the majority of reports of ph1 + acute leukemia are included. compar ... | 1978 | 103315 |
| distribution of trigeminothalamic projection cells in cat and monkey. | | 1979 | 105782 |
| bacteriological study on the saliva of healthy adults. | | 1979 | 106144 |
| [biological control of vectors of human and tropical diseases. present means and prospects (author's transl)]. | biological control is "direct or indirect use of natural enemies of the injurious species to increase its mortality" (w.h.o., 1963). the more and more frequent apparition of resistant insects populations, the fears as regards the environment, the increase cost of hydrocarbur products and also some technic and operational difficulties to stop transmission by the use of only insecticide pulvérisation, impose this process. nevertheless, practic use of natural enemies of vectors is yet unusual in sp ... | 1978 | 106214 |
| mutagenicity of benzotrichloride and related compounds. | benzotrichloride (btc), benzal chloride (bdc), benzyl chloride (bc) and benzoyl chloride (boc) were surveyed for their mutagenicity in microbial systems such as rec-assay using bacillus subtilis and reversion assays using e. coli wp2 and ames salmonella ta strains with or without metabolic activation in vitro. btc and bdc required metabolic activation for their mutagenic activities in several strains of e. coli and salmonella. the mutagenic metabolites of these compounds may not have been produc ... | 1978 | 106269 |
| mutagenic effects of thioacetamide in drosophila melanogaster. | thioacetamide, which is carcinogenic in mice and rats, has been reported as negative in ames's test on salmonella his- with and without liver microsomal fraction (s 9 mix). tests on drosophila reported here showed a significant increase in sex-linked recessive lethals after treatment with thioacetamide, both after injection and after feeding of males. | 1978 | 106270 |
| familial hyperphosphatasemia: diagnosis in early infancy and response to human thyrocalcitonin therapy. | familial hyperphosphatasemia is an uncommon hereditary disorder of membranous bone with concurrent overproduction and overdestruction of bone and bone collagen by osteocytes. this process does not allow normal maturation into compact lamellar bone. two cases of severely affected children are presented which demonstrate that the condition can be diagnosed in early infancy by abnormalities in the long bones. at this stage the skull may appear normal and the characteristic thickening of the calvari ... | 1979 | 106682 |
| raised intracranial pressure: its management in neisseria meningitidis meningoencephalitis. | further reduction in morbidity and mortality from bacterial meningitis is unlikely to stem from innovations in antibiotic management. emphasis must therefore be directed toward prevention, more rapid diagnosis, and improved therapy for maintaining the integrity of the cns. raised intracranial pressure is frequently associated with bacterial meningitis. in selected patients with bacterial meningitis and clinical evidence of elevated intracranial pressure, monitoring and aggressive treatment of th ... | 1979 | 106721 |
| production, purification, and properties of dextransucrase from leuconostoc mesenteroides nrrl b-512f. | the production of dextransucrase from leuconostoc mesenteroides nrrl b-512f was stimulated 2-fold by the addition of 0.005% of calcium chloride to the medium; levansucrase levels were unaffected. dextransucrase was purified by concentration and dialysis of the culture supernatant with a bio-fiber 80 miniplant, and by treatment with dextranase followed by chromatography on bio-gel a-fm. a 240-fold purification, with a specific activity of 53 u/mg, was obtained. contaminating enzyme activities of ... | 1979 | 106966 |
| histochemical localization of glycosidases in dog epididymis. | the histochemical localization of five glycosidases was studied in the epididymis of mature dogs. beta-galactosidase showed a distinct to strong reaction in the epithelium of the ductuli efferentes and throughout the whole length of the ductus epididymidis. beta-n-acetylglucosaminidase reactivity was weak in the initial segment, but increased significantly in the middle and terminal segment. the maximum beta-glucuronidase activity was found in the ductuli efferentes and in the initial segment. t ... | 1979 | 107150 |
| [lactic acid bacteria and streptococci of the human stomach normally and in pathological processes]. | lactic acid bacteria were either absent or present in negligent quantities in the stomach of apparently healthy man. but in case of various pathological processes in the stomach and duodenum lactic acid bacteria accumulated (up to a million cells per 1 ml of gastric contents). under these conditions coccal forms sharply prevailed over the bacillary ones. in patients with chronic gastritis and peptic ulcer of the stomach the most frequent were streptococcus faecalis and its variants; in oncologic ... | 1979 | 107685 |
| mutagenesis of free and intracellular cyanophage as-1 by ultraviolet, n-methanyl-n'-nitro-n-nitrosoguanidine and acriflavine. | mutagenic actions of ultraviolet irradiation (uv), n-methyl-n'-nitro-n-nitrosoguanidine (mnng) and acriflavine (photodynamic) were tested in free and intracellular phage as-1 infecting anacystis nidulans iu625. spontaneous and induced mutations with particular reference to host range (h) and minute plaque formation (m) were investigated. the spontaneous mutation frequencies varied from 10(-9) to 10(-8) and from 2 x 10(-5) to 2 x 10(-4) for h and m mutants respectively. uv was efficient in induci ... | 1979 | 108592 |
| hypnotic susceptibility to various depressants in rats selected for differential ethanol sensitivity. | ma rats, bred for greater motor impairment following subhypnotic doses of ethanol, were found to be more sensitive to the hypnotic effects of phenobarbital and chloral hydrate than were la rats. in addition, the previously reported finding of a difference between the two lines of rats in duration of loss of righting reflex following a hypnotic dose of ethanol was replicated. the results are discussed in terms of a phenotypic difference in central nervous systems sensitivity to a range of sedativ ... | 1979 | 108710 |
| data on chromosome 1 loci fy, pgm1, sc, umpk, rh, pgd, and eno1: two-point lods, r:nr counts, multipoint information, and map. | | 1978 | 110524 |
| the dynamics of testosterone and dihydrotestosterone metabolism in the adult male rhesus monkey. | mcrs of testosterone (t) and 5 alpha-dihydrotestosterone (dht) were determined in the morning and evening in four chronically chaired adult rhesus monkeys (macaca mulatta) by means of steady state infusion of [4-14c]testosterone and [1,2-3h]dihydrotestosterone. morning and evening mcrs were comparable for both t and dht, averaging 169 and 212 ml/min for t and 93 and 108 ml/min for dht, respectively, indicating that temporal differences in blood steroid concentrations are the result of changes in ... | 1979 | 110580 |
| [laboratory diagnosis of group d streptococcus]. | after taxonomic listing of group d streptococci, the authors discuss the methodology of the bacteriologic diagnosis of infections produced by these bacteria, showing the way in which the pathological products are collected, the strains isolated and seeded. the strains are identified in two stages: (1) group identification by group antigen identification, resistance to oxacillin, development at +45 degrees, development on bilaesculin media; (2) species and variety identification by biochemical, p ... | 1979 | 111334 |
| the radiographic diagnosis of heart disease in the dog. | | 1975 | 123974 |
| common skin problems in cats. | | 1975 | 124816 |
| staphylococcal micrococcins. i. isolation of antibiotic-producing strains. | seven strains belonging to the family micrococcaceae and possessing strong antagonistic properties against staph. aureus and several other gram-positive bacteria, have been isolated. the strains were characterized and identified, phage typed, tested for antibiotic-sensitivity and antagonistic activities. two patients of inhibition were noted. | 1975 | 126688 |
| prediction of outcome of human heart transplantation based on lymphocyte tissue culture studies. | | 1975 | 129879 |
| spasticity: actions of selectivity activated group ii muscle spindle afferents on extensor motoneurons. | | 1975 | 129880 |
| [importance of hygiene in the prevention of infectious atrophic rhinitis]. | | 1975 | 129905 |
| effect of a mild exercise program on myocardial function and the development of hypertrophy. | cardiac function and the development of myocardial hypertrophy were studied in rats conditioned by an exercise program consisting of 8 wk of running on a treadmill. at the end of the training period a group of exercised and sedentary rats was subjected to hemodynamic evaluation under general anesthesia. except for a slight elevation in the heart rates of the exercised animals there were no significant differences between the exercised and sedentary rats at rest. following an increase in afterloa ... | 1979 | 154493 |
| suppression of cell-mediated immune reactions by monosaccharides. | various monosaccharides have been shown capable of inhibiting lymphokine activity in in vitro assay systems. in this study, we demonstrate that l-fucose can inhibit the ability of lymphokine-containing supernatants to induce skin reactions or cause reductions in the macrophage content of peritoneal exudates. moreover, l-fucose can inhibit the cutaneous delayed hyoersensitivity reaction and the peritoneal macrophage disappearance reaction (mdr) induced by antigen in actively immunized guinea pigs ... | 1979 | 156214 |
| circulation of pathogenic staphylococci phage types during the last 23 years in romania. | | 1978 | 161497 |
| experimental evaluation of anterior chamber lenses fixated with dacron. | four new designs for anterior chamber intraocular lenses using dacron fibers to achieve fixation were studied. after implantation in rabbit eyes, no lens dislocation occurred. postmortem examination found firm dacron adherence to iris tissue in all eyes. clinically, no difference was noted in the amount of inflammation in eyes receiving a lens with or without dacron. one new lens design was found to have substantial advantages over the other three models. however, a high incidence of corneal ede ... | 1978 | 162544 |
| effect of parathyroid hormone and adenosine 3',5'-monophosphate on renal carbonic anhydrase. | | 1975 | 164869 |
| deoxyribinucleic acid-binding proteins in virus-transformed cell lines. | the synthesis of proteins with affinity for dna has been studied in clones of a syrian hamster cell line (nil) and subclones of this line transformed by polyoma virus (nil-py) or hamster sarcoma virus (nil-hsv). the results show that the synthesis of dna-binding proteins in nil and in its virus-transformed derivatives nil-py and nil-hsv is very similar in exponentially growing cells, but in dense culture there is a very significant difference in the level of a protein (p8), which is much higher ... | 1975 | 165180 |
| a kinetic analysis of the estrogen receptor transformation. | the rate of the 4 to 5 s estrogen-binding protein (ebp) in vitro transformation was measured by sucrose gradient centrifugation analysis. the temperature-activated 4 to 5 s ebp transformation is found to be highly reproducible without loss of [3h]estradiol-binding activity in a buffer containing an excess of [3h]estradiol, 40 mm tris, 1 mm dithiothreitol, and 1 m urea at ph 7.4. the presence of [3h]estradiol is necessary for the 4 to 5 ebp transformation. a kinetic analysis of the 4 to 5 ebp tra ... | 1975 | 165190 |
| studies on the composition of pig serum lipoproteins. isolation and characterization of different apoproteins. | 1. different lipoprotein density fractions from pig serum were isolated by phosphotungstate precipitation followed by purification in the preparative ultra-centrifuge. 2. the protein part of very low density lipoproteins was composed of approximately 52 percent lipoprotein b apoprotein and the rest of lipoprotein c ii apoprotein and other as yet unidentified peptides. 3. the protein moiety of low density lipoproteins consisted primarily of lipoprotein b apoprotein (over 95 percent); the amino ac ... | 1975 | 166686 |
| effects of biogenic amines on the concentration of adnosine 3',5'-monophosphate in bovine superior cervical ganglion. | | 1975 | 168507 |
| ultrastructural changes in rat hepatocytes following acute methyl mercury intoxication. | | 1975 | 169128 |
| regulation of dna synthesis by guanosine-5'-diphosphate, cyclic guanosine-3',5'-monophosphate, and cyclic adenosine-3', 5'-monophosphate in mouse lymphoid cells. | | 1975 | 169132 |
| effect of some phosphodiesterase inhibitors on two different preparations of adenosine 3',5'-monophosphate phosphodiesterase. | | 1975 | 169139 |
| effect of cirrhosis on pulmonary fibrogenesis caused by silica dust in rats. | the cirrhosis in male albino rats was produced after repeated subcutaneous injections of carbon tetrachloride, in dosage schedule as described. a fully developed cirrhosis was observed after 150 days. the silicosis in lungs was produced after single intratracheal injection of silica dust of particle size less than 5 microns and studied up to 180 days. the effect of damaged liver on the development of silicosis in the lungs was studied. it was found that pulmonary fibrosis caused by silica dust a ... | 1975 | 183970 |
| determination of the hydride transfer stereospecificity of nicotinamide adenine dinucleotide linked oxidoreductases by proton magnetic resonance. | a facile proton magnetic resonance technique is described for the determination of the coenzyme stereospecificity during hydride transfer reactions catalyzed by pyridine nucleotide dependent oxidoreductases. the reliability of this technique was demonstrated by examining the coenzyme stereospecificity of lactate, malate, and 3-phosphoglycerate dehydrogenases, which are known to be a-stereospecific enzymes, as well as triosephosphate and octopine dehydrogenases, which are known to be b-stereospec ... | 1976 | 186097 |
| dimeric ("big") human placental lactogen. immunological and biological activity. | dimeric ("big") human placental lactogen has been isolated in near homogeneous form from placental tissue. it consists of a disulfide-linked (stable) form and a noncovalently associated (unstable) form of the native hormone. the two forms were separated by exposure to denaturing conditions and resolution by gel exclusion chromatography. both forms retained immunological activity, ability to bind mammary membranes, and ability to induce mammary n-acetyllactosamine synthetase in vitro. on a molar ... | 1977 | 195629 |
| liquoid-induced arterial lesions and the generalized shartzman reaction. | | 1977 | 202152 |
| effect of starvation on hepatic glycogen metabolism and glucose homeostasis. | the effects of starvation on the hepatic glycogen synthase and phosphporylase systems were sequentially assessed in fed and 24-120-hr-fasted rats. enzymic changes before and after glucose were correlated with simultaneous measurements of hepatic cyclic amp and glycogen concentrations and glucose, insulin, and glucagon concentrations in the portal vein plasma. fasting caused parallel changes in plasma glucose and hepatic glycogen concentrations with decreases by 24 hr and subsequent increases, wh ... | 1978 | 203823 |
| insulin regulation of leydig cell luteinizing hormone receptors. | the effect of experimentally induced diabetes on the luteinizing hormone-human chorionic gonadotropin receptors in rat testes was examined. pancreatectomy and streptozotocin diabetes the gonadotropin receptors and the administration of insulin restored the binding capacity to normal values. no differences in the association constant as well as the sedimentation coefficients of the luteinizing hormone-human chorionic gonadotropin receptor complexes were observed between normal and diabetic animal ... | 1978 | 204651 |
| immunoelectrophoretic study of sendae virus envelope antigens with hyperimmune sera prepared in different laboratory animals. | at similar hai titers rabbit sera reveal a larger number of fractions (ten) in immunoelectrophoretic analysis of sendai virus envelopes than do guinea pig sera, which only yield three distinct fractions. these differences are observed in both rocket and crossed immunoelectrophoresis, and are more marked in the latter. the number of fractions revealed does not differ in relation to the antigen used for hyperimmunization (intact sendai virus, triton-disrupted virus or envelope fractions extracted ... | 1978 | 205040 |
| characterization of a unique cell line (laz 221) from human acute lymphocytic ("null" cell) leukemia. | a unique human cell line designated laz 221 has been established from the peripheral blood of a patient with acute lymphocytic leukemia of the "null" cell type. the cell line does not possess the epstein-barr virus nuclear antigen and has a karyotype of 45,xx,-9,-12,+(9q12q). both the established cell line and the patient's uncultured blast cells share the same phenotypic markers. they both lack t-cell markers. they fail to form sheep erythrocyte rosettes and do not react with t-cell-specific an ... | 1978 | 205365 |
| [transplantation of human tumors to athymic mice]. | | 1978 | 209637 |
| changes in rat hypothalamic content of corticotrophin-releasing factor (crf) activity, plasma acth and corticosterone under stress and the effect of cycloheximide. | | 1978 | 211772 |
| purification and some structural properties of adenylate kinase from leuconostoc mesenteroides (lactobacteriaceae). | | 1978 | 213322 |
| evidence for an essential lysine in glucose-6-phosphate dehydrogenase from leuconostoc mesenteroides. | 1. pyridoxal 5'-phosphate inhibits glucose-6-phosphate dehydrogenase from leuconostoc mesenteroides reversibly which ki equals 0.04-0.06 mm. 2. this inhibition is competitive with respect to glucose 6-phosphate and non-competitive with respect to nadp+ or nad+. interaction between enzyme and excess pyridoxal 5'-phosphate follows pseudo-first-order kinetics and indicates that one molecule of inhibitor reacts with each active unit of enzyme. 3. substrate and coenzyme protect the enzyme from inhibi ... | 1975 | 236186 |
| glucose-6-phosphate dehydrogenase from leuconostoc mesenteroides. | | 1975 | 236437 |
| purification and characterization of two activities of the intracellular dextransucrase from leuconostoc mesenteroides nrrl b-1299. | dextransucrase (sucrose: 1,6-alpha-d-glucan 6-alpha-glucosyltransferase ec 2.4.1.5) activity of leuconostoc mesenteroides nrrl b-1299 cells has been purified by adsorption on hydroxyapatite, followed by chromatographies on deae-cellulose and deae-sephadex. the enzyme activity was readily separated into two principal forms of the enzyme, i and ii, by deae-cellulose chromatography. both enzymes i and ii were purified to an electrophoretically homogeneous state in which the relative enzyme activiti ... | 1975 | 238637 |
| formation of the four isomers of hen egg white lysozyme containing three negative disulfide bonds and one open disulfide bond. | reduced partially carboxymethylated hen egg white lysozyme (mucopeptide n-acetylmuramoylhydrolase; ec 3.2.1.17) (approximately 0.8 mol of [1-(14)c]carboxymethyl groups) was air oxidized at ph 8.0 and 37 degrees in the presence of 1.5 mm 2-mercaptoethanol for 36 hr. gel filtration of this product gave the lower (native) and higher hydrodynamic volume forms, both containing radioactivity (approximately 35 and 65%, respectively). ion exchange chromatography of the lower hydrodynamic volume forms yi ... | 1977 | 267931 |
| scanning and transmission electron microscopy of enamel formation and structure in the molar teeth of rats. | | 1978 | 278569 |
| x-linked recessively inherited non-specific mental retardation. report of a large family. | a large kindred is described in which 22 males and 3 females show non-specific mental retardation with impaired speech. an x-linked recessive is the most likely mode of inheritance of this condition. similar families have been described in the literature, characteristic physical abnormalities are absent and performance i.q. tends to be higher than verbal i.q. this possible heterogenous condition may be a major individual cause of mental deficiency in males, and may account for the excess of male ... | 1977 | 305754 |
| biochemical strain characterization of trypanosoma cruzi by restriction endonuclease cleavage of kinetoplast-dna. | | 1977 | 321246 |