| microorganisms and characteristics of laban. | laban had a titratable acidity of about 1.0%, a ph of 4.25, an ethanol content of 1.25%, and contained 4.2 mug acetaldehyde and 34 mug acetoin/ml. there was no diacetyl. five microorganisms, classified as streptococcus thermophilus, lactobacillus acidophilus, leuconostoc lactis, kluyveromyces fragilis, and saccharomyces cerevisiae, were responsible for the fermentation. streptococcus thermophilus and l. acidophilus were responsible for acid production with s. thermophilus producing acid more rap ... | 1976 | 2630 |
| citric acid metabolism in hetero- and homofermentative lactic acid bacteria. | the effect of citrate on production of diacetyl and acetoin by four strains each of heterofermentative and homofermentative lactic acid bacteria capable of utilizing citrate was studied. acetoin was quantitatively the more important compound. the heterofermentative bacteria produced no acetoin or diacetyl in the absence of citrate, and two strains produced traces of acetoin in its presence. citrate stimulated the growth rate of the heterofermentative lactobacilli. acidification of all heteroferm ... | 1976 | 5054 |
| the toxicity of sulphur dioxide towards certain lactic acid bacteria from fermented apple juice. | | 1976 | 5394 |
| purification and properties of the extracellular dextransucrase from leuconostoc mesenteroides nrrl b-1299. | dextransucrase ec 2.4.1.5 activity from cell-free culture supernatant of leuconostoc mesenteroides nrrl b-1299 was purified by (nh4)2so4 fractionation, adsorption on hydroxyapatite, chromatography on deae-cellulose and gel filtration on sephadex g-75. the extracellular enzyme was separated into two principal forms, enzymes i and n, and the latter was shown to be an aggregated form of the protomer, enzyme i. enzymes i and n were both electrophoretically homogeneous and their relative activities r ... | 1976 | 8441 |
| glucose-6-phosphate dehydrogenase from leuconostoc mesenteroides. interaction of the enzyme with coenzymes and coenzyme analogs. | | 1976 | 11751 |
| production of l-aspartic acid by microbial cells entrapped in polyacrylamide gels. | | 1976 | 15193 |
| identification of essential arginine residues in glucose-6-phosphate dehydrogenase from leuconostoc mesenteroides. | | 1977 | 16915 |
| lactostrepcins--acid bacteriocins produced by lactic streptococci. | all 47 non-nisin producing strains of streptococcus lactis and 12/13 strains of str. lactis subsp. diacetylactis examined produced bacteriocins, for which the term lactostrepcins is suggested. seven strains of str. cremoris examined produced no bacteriocins active against 3 lactic streptococci strains used as indicators. the strains examined were divided into 3 groups: i, those producing lactostrepcins active against only one streptomycin resistant mutant of str. lactis 60 indicator strain; ii, ... | 1978 | 27542 |
| an improved enzymatic cycle for nicotinamide-adenine dinucleotide phosphate. | | 1978 | 30332 |
| simultaneous analysis of nad- and nadp-linked activities of dual nucleotide-specific dehydrogenases. application to leuconostoc mesenteroides glucose-6-phosphate dehydrogenase. | a method is described which enables one to assay simultaneously the nad- and nadp-linked reactions of dehydrogenases which can utilize both coenzymes. the method is based on the fact that the thionicotinamide analogs of nadh and nadph absorb light maximally at 400 nm, a wavelength sufficiently far removed from the absorbance maximum of nadh and nadph to permit measurements of the simultaneous reduction of nad+ (or nadp+) and the thionicotinamide analog of nadp+ (or nad+). application of the meth ... | 1979 | 35541 |
| regulation of coenzyme utilization by the dual nucleotide-specific glucose 6-phosphate dehydrogenase from leuconostoc mesenteroids. | | 1979 | 42353 |
| influence of indicating enzyme reaction on apparent creatine kinase activity creatine kinase in serum, vii. | comparison of the indicative systems yeast glucose-6-phosphate dehydrogenase/nadp+, leuconostoc glucose-6-phosphate dehydrogenase/nadp+ and leuconostoc glucose-6-phosphate dehydrogenase/nad+ showed excellent correlation and no differences in apparent creatine kinase activity with the two methods using nadp+. by using nad+ with the leuconostoc enzyme enzyme relative recovery of apparent creatine kinase activity is lower due to interference of other serum constituents. the mean value of the relati ... | 1979 | 44720 |
| influence of molecular structure on the tolerogenicity of bacterial dextrans. i. the alpha1--6-linked epitope of dextran b512. | native dextran b512 is a near-linear glucose polymer with 96 per cent alpha1--6 and 4 per cent alpha1--3 linkages and a molecular weight (mol. wt) of 8 x 10(7). sheep rbc sensitized with its o-stearoyl derivative (prepared by a modified method) have been used satisfactorily in direct pfc assays. b512 immunizes balb/c mice optimally with doses of 1--10 mug and produces b-cell tolerance with 1 mg upwards. the specificity of the response determined by pfc inhibition analysis, is directed towards an ... | 1975 | 52612 |
| influence of molecular structure on the tolerogenicity of bacterial dextrans. ii. the alpha1--3-linked epitope of dextran b1355. | dextran b1355 is a branched glucose polymer containing 57 per cent alpha1--6, 35 per cent alpha1--3 and 8 per cent alpha1--2/1--4 linkages. direct pfc responses to b1355 can be measured with sheep rbc sensitized with its o-stearoyl or palmitoyl derivative, and, as shown by inhibition analysis, are specific for an eptiope which is dependent on alpha1--3 linkages. b1355 is a potent immunogen in balb/c mice producing peak pfc levels which approach 10(6) per spleen following an optimal dose of 1 mg. ... | 1975 | 52613 |
| influence of molecular structure of the tolerogenicity of bacterial dextrans. iii. dissociation between tolerance and immunity to the alpha1--6- and alpha1--3-linked epitopes of dextran b1355. | dextran b1355 induces a direct pfc response detectable with dextran b512-sensitized red cells which is directed towards an alpha1--6-linked glucose epitope. this response is distinguishable from the alpha1--3-linked specificity assayed by homologous sensitization in that: (a) it is totally suppressed in donors previously rendered tolerant of b512; (b) the pfc are sensitive to inhibition by b512 and isomaltohexaose. the alpha1--6 epitope of b1355 is less immunogenic in balb/c mice than that with ... | 1975 | 52614 |
| influence of molecular structure on the tolerogenicity of bacterial dextrans. iv. epitope size recognition and genetic resistance to alpha (1 leads to 3) glucosyl tolerance induction by dextran b1355. | | 1977 | 70361 |
| the use of an oscillating-tube densitometer as a tool in enzyme kinetics. determination of the influence of sodium ascorbate on invertase, dextransucrase and dextranase. | the use of a commercial oscillating-tube densitometer with an accuracy of 4 . 10(-7) g/cm3 for the determination of enzyme-kinetics constants is tested. this method is applied to the investigation of the influence of vitamin c (sodium ascorbate) on the glycolytic enzymes invertase, dextransucrase and dextranase. invertase is inhibited uncompetitively, dextransucrase non-competitively. there is no significant effect of the vitamin on dextranase. the comparison of the mechanisms of the three enzym ... | 1979 | 95345 |
| lactase activity of microorganisms. | sixty-two strains of yeasts, molds and bacteria were screened for lactase (beta-d-galactosidase) activity. strains exhibiting the enzyme activity were evaluated for cell yield as well as enzyme units available per litre of the medium, per g cell dry weight and per mg protein of their cell-free extracts. the molds exhibited lowest enzyme activity but highest cell yields, bacteria produced lowest cell yield and maximum enzyme activity. cultures exhibiting very high activity among yeasts were sacch ... | 1978 | 97187 |
| estimation of antibodies specific for dextran. | methods are described for the isolation and characterization of picogram quantities of anti-dextran antibodies. 14c-dextrans produced by using the dextransucrases of leuconostoc mesenteroides strains b1355 and b512 were used in a radioimmunoassay. the specificity of this assay was verified by using cell cytoplasmic lysates from mouse plasmacytomas, j558 (anti-alpha 1 leads to 3 dextran) and w3129 (anti-alpha 1 leads to 6 dextran). dextran produced by strain b1355 and insolubilized with epichloro ... | 1978 | 99480 |
| an assessment of methods used to determine protein quality. | | 1978 | 104444 |
| production, purification, and properties of dextransucrase from leuconostoc mesenteroides nrrl b-512f. | the production of dextransucrase from leuconostoc mesenteroides nrrl b-512f was stimulated 2-fold by the addition of 0.005% of calcium chloride to the medium; levansucrase levels were unaffected. dextransucrase was purified by concentration and dialysis of the culture supernatant with a bio-fiber 80 miniplant, and by treatment with dextranase followed by chromatography on bio-gel a-fm. a 240-fold purification, with a specific activity of 53 u/mg, was obtained. contaminating enzyme activities of ... | 1979 | 106966 |
| [lactic acid bacteria and streptococci of the human stomach normally and in pathological processes]. | lactic acid bacteria were either absent or present in negligent quantities in the stomach of apparently healthy man. but in case of various pathological processes in the stomach and duodenum lactic acid bacteria accumulated (up to a million cells per 1 ml of gastric contents). under these conditions coccal forms sharply prevailed over the bacillary ones. in patients with chronic gastritis and peptic ulcer of the stomach the most frequent were streptococcus faecalis and its variants; in oncologic ... | 1979 | 107685 |
| acetaldehyde: an intermediate in the formation of ethanol from glucose by lactic acid bacteria. | group n streptococci formed acetaldehyde and ethanol from glucose. as the enzymes aldehyde dehydrogenase, phosphotransacetylase and acetate kinase were present this would enable these organisms to reduce acetyl-coa to acetaldehyde and convert acetyl-coa to acetyl phosphate and acetate. a pentose phosphate pathway which converted ribose-5-phosphate to glyceraldehyde-3-phosphate was also present. acetaldehyde could not be formed via the hexose monophosphate shunt or by direct decarboxylation of py ... | 1976 | 177470 |
| determination of the hydride transfer stereospecificity of nicotinamide adenine dinucleotide linked oxidoreductases by proton magnetic resonance. | a facile proton magnetic resonance technique is described for the determination of the coenzyme stereospecificity during hydride transfer reactions catalyzed by pyridine nucleotide dependent oxidoreductases. the reliability of this technique was demonstrated by examining the coenzyme stereospecificity of lactate, malate, and 3-phosphoglycerate dehydrogenases, which are known to be a-stereospecific enzymes, as well as triosephosphate and octopine dehydrogenases, which are known to be b-stereospec ... | 1976 | 186097 |
| purification and some structural properties of adenylate kinase from leuconostoc mesenteroides (lactobacteriaceae). | | 1978 | 213322 |
| [new species of lactic acid bacteria, described in the ussr, in the light of modern identification schemes]. | | 1975 | 235331 |
| evidence for an essential lysine in glucose-6-phosphate dehydrogenase from leuconostoc mesenteroides. | 1. pyridoxal 5'-phosphate inhibits glucose-6-phosphate dehydrogenase from leuconostoc mesenteroides reversibly which ki equals 0.04-0.06 mm. 2. this inhibition is competitive with respect to glucose 6-phosphate and non-competitive with respect to nadp+ or nad+. interaction between enzyme and excess pyridoxal 5'-phosphate follows pseudo-first-order kinetics and indicates that one molecule of inhibitor reacts with each active unit of enzyme. 3. substrate and coenzyme protect the enzyme from inhibi ... | 1975 | 236186 |
| d-mannitol dehydrogenase from leuconostoc messenteroides. | | 1975 | 236430 |
| glucose-6-phosphate dehydrogenase from leuconostoc mesenteroides. | | 1975 | 236437 |
| l-ribulose-5-phosphate 4-epimerase from aerobacter aerogenes. | | 1975 | 236467 |
| d-fructose (d-mannose) kinase. | | 1975 | 237199 |
| purification and characterization of two activities of the intracellular dextransucrase from leuconostoc mesenteroides nrrl b-1299. | dextransucrase (sucrose: 1,6-alpha-d-glucan 6-alpha-glucosyltransferase ec 2.4.1.5) activity of leuconostoc mesenteroides nrrl b-1299 cells has been purified by adsorption on hydroxyapatite, followed by chromatographies on deae-cellulose and deae-sephadex. the enzyme activity was readily separated into two principal forms of the enzyme, i and ii, by deae-cellulose chromatography. both enzymes i and ii were purified to an electrophoretically homogeneous state in which the relative enzyme activiti ... | 1975 | 238637 |
| enzyme multiplied immunoassay of phenobarbital by lkb reaction rate analyser: a comparison with analysis by gas-liquid chromatography. | a procedure for the enzyme multiplied immunoassay of phenobarbital using the lkb reaction rate analyser is described. the temperature optimum, linearity, specificity, and precision of the assay are reported. a highly significant correlation coefficient is obtained when the technique is compared with gas-liquid chromatography by analysing human sera containing a range of phenobarbital levels. | 1977 | 332043 |
| immunological properties of a high molecular weight component from yeast cell autolysate in dogs and evaluation of its potential role in human dextran reactions. | a high molecular weight component (hmc) of autolysate from saccharomyces cerevisiae yeast cells was prepared. hmc was found to be immunogenic in dogs, inducing hemagglutinating antibody formation. upon hmc challenge of immunized dogs, systemic anaphylactoid reactions were observed in 4/5 animals. the most prominent symptom was decreased cardiac output. decrease in mean arterial pressure and increase in pulmonary arterial pressure were also observed. consumption of total serum complement activity ... | 1977 | 336373 |
| dextran as a plasma volume substitute. | | 1978 | 351632 |
| biochemical characteristics of streptococcus species. | | 1978 | 364662 |
| in vitro antimicrobial activity of some cyclic hydroxamic acids and related lactams. | against enterobacter aerogenes 13048, serratia marcescens 13880, klebsiella pneumoniae 10031, pseudomonas aeruginosa 10145, escherichia coli 9723, lactobacillus casei 7469, lactobacillus plantarum 8014, leuconostoc dextranicum 8086, and streptococcus faecalis 8043, the mean minimal inhibitory concentrations of three cyclic hydroxamic acids, 3-amino-3,4-dihydro-1-hydroxycarbostyril, the 6-chloro analog, and the 7-chloro analog, were 0.6, 0.6, and 0.2 micrograms/ml, and those of the corresponding ... | 1978 | 400828 |
| lipoquinones of some bacteria and mycoplasmas, with considerations on their functional significance. | in a comparative study the lipoquinones of some chemoorganotrophic, facultatively aerobic bacteria, and representative acholeplasma, mycoplasma, spiroplasma, and thermoplasma strains were investigated. the quinones were partly purified by preparative thin layer chromatography of lipid extracts, and characterized by their difference spectra (reduced minus oxidized) and rf values. respiring bacteria expectedly contained benzoquinones and/or naphthoquinones in micromolar concentrations whereas some ... | 1977 | 413478 |
| rapid automated turbidimetric assay for chlortetracycline hydrochloride, using leuconostoc mesenteroides as the test organism. | a rapid turbidimetric assay has been developed for chlortetracycline hydrochloride (ctc-hcl) in finished animal feeds and feed supplements, using leuconostoc mesenteroides as the test organism. two modifications are presented: the incubation period of modification 1 is 2.5 hr and the sensitivity of the assay is 0.03 microgram ctc-hcl/assay tube. modification 2 has a sensitivty of 0.01 microgram ctc-hcl/assay tube and requires an incubation period of 3.5 hr. for 21 feed formulations, the turbidim ... | 1979 | 422497 |
| biological properties of transition-metal organometallic compounds. 3. beta-ferrocenylalanine. | | 1979 | 430480 |
| syntheses of glycosyl derivatives of pantothenic acid and pantetheine. | | 1979 | 440102 |
| dextran biosynthesis and dextransucrase production by continuous culture of leuconostoc mesenteroides. | leuconostoc mesenteroides nrrl b-512(f) was grown in continuous culture under conditions of energy-limited growth. the extracellular enzyme dextransucrase (sucrose: 1,6-alpha-d-glucan 6-alpha-glucosyltransferase ec 2.4.1.5), was not detected in glucose- or maltose-limited cultures. under conditions of sucrose-limited growth, the enzyme activity of the cell-free culture supernatant increased with increasing dilution rate only after the critical concentration of enzyme inducer (sucrose) in the che ... | 1979 | 454805 |
| distribution of the phosphoenolpyruvate:glucose phosphotransferase system in fermentative bacteria. | a number of selected fermentative bacteria were surveyed for the presence of the phosphoenolpyruvate:glucose phosphotransferase system, with particular attention to those organisms which ferment glucose by pathways other than the embden-meyerhof-parnas pathway. the phosphoenolpyruvate:glusoe phosphotransferase system was found in all homofermentative lactic acid bacteria tested that ferment glucose via the embden-meyerhof-parnas pathway, but in none of a group of heterofermentative species of la ... | 1979 | 457606 |
| pronounced hydrolysis of highly branched dextrans with a new type of dextranase. | | 1978 | 623663 |
| the mechanism of acceptor reactions of leuconostoc mesenteroides b-512f dextransucrase. | reactions of dextransucrase and sucrose in the presence of sugars (acceptors) of low molecular weight have been observed to give a dextran of low molecular weight and a series of oligosaccharides. the acceptor reaction of dextransucrase was examined in the absence and presence of sucrose by using d-[14c]glucose, d-[14c]fructose, and 14c-reducing-end labeled maltose as acceptors. a purified dextransucrase was preincubated with sucrose, and the resulting d-fructose and unreacted sucrose were remov ... | 1978 | 647705 |
| growth inhibition of streptococcus mutans and leuconostoc mesenteroides by sodium fluoride and ionic tin. | sodium fluoride caused inhibition of growth rate and growth levels of streptococcus mutans with glucose as the primary energy and carbon source. stannous fluoride increased growth lag nad caused a much greater inhibition of growth rate than did sodium fluoride. neither compound was found to be bactericidal when culture viability was measured after 6 days of incubation. leuconostoc mesenteroides, which lacks a phosphotransferase system for sugar transport, showed less inhibition of growth rate wi ... | 1978 | 655708 |
| calorimetric identification of several strains of lactic acid bacteria. | a microcalorimetric method has been developed for the identification of 9 representative strains of lactic acid bacteria used in the dairy industry. a chemically defined medium containing several carbohydrates gave reproducible and characteristic growth patterns. the technique is proposed as a rapid alternative method for the identification of bacteria selected for commercial use. | 1978 | 711958 |
| synthesis and microbiological activities of beta-(1-chloro-2-naphthyl) alanine and beta-(1-bromo-2-naphthyl) alanine. | beta-(1-chloro-2-naphthyl)alanine and beta-(1-bromo-2-naphthyl) alanine were synthesized by ammonolysis of the corresponding alpha, 1-dihalo-2-naphthalenepropanoic acids derived from 1-nitro-2-naphthylamine by diazotization and condensation with acrylic acid in the presence of cuprous halides. the two analogs as well as the previously reported beta-(2-naphthyl)alanine and beta-(1-naphthyl)alanine were studied as growth inhibitors of escherichia coli 9723, leuconostoc dextranicum 8086, and lactob ... | 1976 | 768475 |
| hot-loop test for the determination of carbon dioxide production from glucose by lactic acid bacteria. | a hot-loop test was found to be more rapid, convenient, and reliable for the detection of carbon dioxide production than were conventional methods such as displacement of agar plugs and precipitation in barium hydroxide. | 1976 | 779651 |
| racemic diastereoisomers of 1-amino-2-hydroxycyclopentanecarboxylic acid. | the synthesis and characterization of the two diastereoisomeric forms of 1-amino-2-hydroxycyclopentanecarboxylic acid have been accomplished. a previously reported synthesis produced a racemic mixture of the threonine analog trans-2-hydroxy-1-aminocyclopentanecarboxylic acid (trans with respect to the hydroxy and carboxyl group). the alternate allothreonine analog was produced by conversion of cyclopentene oxide to trans-2-methoxycyclopentanol, followed by oxidation to 2-methoxycyclopentanone an ... | 1976 | 814238 |
| determination of the isotope distribution in malate-14c with fumarase-negative lactic acid bacteria. | no fumarase activity could be found in whole cells or in cell-free crude extracts from leuconostoc mesenteroides or lactobacillus curvatus. the degradation of l-malate-4-14c by these organisms yielded more than 95% of the label as 14co2. it is therefore recommended that these organisms, rather than lactobacillus plantarum, should be used in the determination of isotope distribution in l-malate-14c, since l. plantarum exhibits a significant fumarase activity and thus randomizes malate prior to th ... | 1977 | 843172 |
| examination and modification of the use of leuconostoc mesenteroides for measurements of the sulfur-containing amino acids from vigna unguiculata. | | 1977 | 858858 |
| ultrastructural surface changes associated with dextran synthesis by leuconostoc mesenteroides. | when leuconostoc mesenteroides ncdo 1875 was grown in mrs broth and fixed for electron microscopy in the presence of ruthenium red, the cell wall appeared as a triple-layered structure similar to other, gram-positive bacteria. when such logarithmic-phase cultures were exposed to sucrose, the appearance and growth of a uniform layer of electron-dense material was evident on the surface of the cell wall. after 2 h in the presence of sucrose, the formation of this surface coat (110 to 130 nm thick) ... | 1977 | 873886 |
| evaluation of a new immunoassay for determination of phenytoin and phenobarbital: results of a european collaborative control study. | the performance of a new enzyme multiplied immunoassay technique (emit) was compared with other current methods, namely gas-liquid chromatography and thin-layer chromatography, for the determination of phenytoin and phenobarbital in serum. forty-three serum samples were sent as unknowns to the participating laboratories for determination. the precision of repeated determinations was very similar for emit and chromatography. there was good agreement among gas chromatography, thin-layer chromatogr ... | 1977 | 891490 |
| the mechanism of dextransucrase action. biosynthesis of branch linkages by acceptor reactions with dextran. | | 1976 | 938046 |
| bacteria within ovules and seeds. | surface-sterilized ovules and seeds of 27 species of plants were cultured in the water of syneresis of a nutrient medium low in agar content. bacteria were obtained from 30% of the ovules, 15% of the seeds of herbaceous plants, 16% of the seeds of woody plants, 5.4% of the overwintered noncereal seeds, and 13.5% of overwintered cereal seeds. in no instance did every ovule or seed of a plant species contain bacteria. no bacteria were obtained from the hard, waxy seeds of mimosa or yellowwood. the ... | 1976 | 984839 |
| immunochemical studies on dextrans: microheterogeneity of dextran nrrl b1397 demonstrated by precipitation with concanavalin a. | | 1976 | 985470 |
| on the mechanism and stereochemistry of the malate-lactate fermentation of leuconostoc mesenteroides. | during the transformation of (2s, 3r) [3-3h]malate to (s) lactate no tritium exchange takes place. the stereochemical course of the decarboxylation studied with (2s, 3r) [3-2h]-malate in 3hoh/h2o and (2s, 3r) [3-3h]malate in 2h2o occurs with retention and is therefore the same as that determined by other authors for malic enzyme from vertebrates and from escherichia coli. the malate-lactate fermentation is a useful procedure to prepare chiral methyl groups on a preparative scale starting from (2 ... | 1976 | 992575 |
| surface coat transformation and capsule formation by leuconostoc mesenteroides ncdo 523 in the presence of sucrose. | when leuconostoc mesenteroides ncdo 523 was grown in mrs browth, electron microscopy of cells fixed in the presence of ruthenium red showed that the cell wall was covered with a thin layer of filamentous material. when mrs-grown cells were resuspended in the same medium supplemented with 3.6% sucrose, this surface coat doubled in thickness and a number of radial thickenings appeared within it. after 3 h, the filamentous component of the surface coat had disappeared leaving only the radial projec ... | 1976 | 1015962 |
| high pressure enzyme kinetics of dextransucrase. | the pressure dependence of enzymatic dextran formation has been observed up to 1000 at for several substrate concentrations. first order denaturation effects could be separated from the thermodynamic effects, which lead to a volume of 30.4 to 44.0 ccm per mole for the formation and -13.6ccm per mole for the activation of the enzyme-substrate complex. denaturation depends on the substrate concentration. this leads to the conslusion that only the free enzyme is denatured, wheras the es complex is ... | 1976 | 1016687 |
| the effect of topical application of dextran on the gingiva of the beagle dog. | dextrans derived from leuconostoc mesenteroides were placed on clinically healthy gingiva of beagle dogs once a day for 21 days. control gingival tissues received saline. both healthy controls and dextran-treated tissues were brushed daily. inflamed control tissues were obtained by allowing plaque to accumulate for 21 days. tissues receiving daily application of dextran solutions developed chronic gingival inflammation but displayed no clinical signs of gingivitis. healthy control gingival tissu ... | 1976 | 1062559 |
| assay for citrovorum factor (nsc-3590) in the presence of methotrexate (nsc-740). | treatment of certain malignancies with high-dose methotrexate/citrovorum factor rescue has recently been adopted as an effective regimen. a microbiologic assay capable of detecting citrovorum factor in the presence of massive amounts of methotrexate has been developed using a strain of pediococcus cerevisiae resistant to methotrexate. the assay described in this paper is an inexpensive and rapid method of studying the distribution kinetics of citrovorum factor. | 1975 | 1081908 |
| synthesis and antibacterial activities of some chloro analogs of 3 amino-3,4-dihydro-1-hydroxycarbostyril. | the effects of a chloro substituent upon the microbiological activities of 3-amino-3,4-dihydro-1-hydroxycarbostyril were determined. the 5-, 6-, and 7-chloro analogs were synthesized by reductive cyclizations of the appropriately chloro-substituted o-nitrophenylalanines, while the 8-chloro analog was obtained from the n-trifluoroacetyl-3-chloro-2-nitrophenylalanine ethyl ester. all of these compounds were observed to inhibit the growth of escherichia coli 9723, leuconostoc dextranicum 8086, and ... | 1975 | 1097694 |
| detection of mannitol formation by bacteria. | a test is described by means of which formation of mannitol from fructose by lactic acid bacteria can be readily detected. the test is based on removal of interference of residual fructose by dehydration with hydrochloric acid followed by thin-layer chromatography. | 1975 | 1101827 |
| interlaboratory comparison of enzymatic methods for serum glucose determination. | this report describes an interlaboratory comparison of enzymatic serum glucose methods as currently applied in several instrumental adaptations. four spectrophotometric, hexokinase-based methods used with the du pont "aca," the abbott "bichromatic analyzer," the aminco "rotochem," and the technicon "autoanalyzer ll" were compared with glucose oxidase-based methods as used with the beckman "glucose analyzer" and the "autoanalyzer ll." we analyzed both normal samples and samples that contained pot ... | 1975 | 1112045 |
| citrate lyase from streptococcus diacetilactis. association with its acetylating enzyme. | citrate lyase (ec 4.1.3.6) was purified 38-fold from cell-free extracts of streptococcus diacetilactis. the enzyme was homogeneous in analytical ultracentrifugation and polyacrylamide gel electrophoresis. the final enzyme preparation contained acetate: hs-citrate lyase ligase--an acetylating enzyme which converts inactive hs-citrate lyase into enzymatically active acetyl-s-citrate lyase. this enzyme activity was purified 25-fold over the crude extract and seemed to be associated with citrate lya ... | 1975 | 1115558 |
| effect of substitution on reactivity of b 512 dextran fractions with anti-b 512 dextran in heterologous passive cutaneous anaphylaxis. | the influence of type and degree of substitution of b 512 dextran fractions on reactivity with rabbit antibodies against unmodified b 512 dextran was studied on heterologous passive cutaneous anaphylaxis in guinea pigs. low degrees of substitution, i.e. 1 substituent group per 7-30 glucose residues, affected reactivity with anti-dextran only slightly or not at all. this was shown for the following substituents: sulphate, carboxymethyl, phosphate, diethylaminoethyl, hydroxypropyl, butyryl, capryl ... | 1975 | 1120617 |
| [use of a freeze-drief culture of leuconostoc mesenteroides for the synthesis of dextran]. | the influence of a prolonged storage of the freeze-dried culture leuconostoc mesenteroides strain sf-4 on its capacity for the dextrane synthesis has been studied. the culture has maintained its normal viability for 12 years (the observation time) without changes in the cell morphology and capacity for the dextrane synthesis. the structure of dextrane synthesized by the revived culture l. mesenteroides has been similar to that of dextrane synthesized by the culture l. mesenteroides preserved via ... | 1975 | 1129227 |
| citrate utilization in milk by leuconostoc cremoris and streptococcus diacetilactis. | citrate utilization and diacetyl, acetoin and acetaldehyde production by 2 strains each of leuconostoc cremoris and streptococcus diacetilactis in milk were studied. with the leuconostoc bacteria no growth and little citrate utilization occurred unless a stimulant (yeast extract) was present, when complete utilization of citrate without concomitant production of diacetyl or acetoin was obtained. the additon of mn2+ stimulated growth resulted in diacetyl and acetoin production. destruction of dia ... | 1975 | 1173068 |
| [influence of products of thermophilous methane fermentation on the mixed lactic acid bacterial culture]. | the effect of products resulting from thermophilous methane fermentation on the accumulation and ratio of different lactic acid bacteria (str. lactis, str. cremoris, str. diacetilactis, str. diacetil. var. acetoinicus, leuc. citrovorum8 l. plantarum) was investigated upon their combined cultivation in milk and serum. in combined cultivation of the streptococcal culture the rate of growth of a single strain differed from that in separate cultivation. str. cremoris was accumulated with the highest ... | 1975 | 1208408 |
| [fermentative degradation of 2-14c-mannose with leuconostoc mesenteroides (author's transl)]. | mannose-2-14c has been fermented by leuconostoc mesenteroides, co2 ethanol and d-lactic acid were formed in a molar ratio of 1:1:1. a small amount of acetic acid was found as by-product. it could easily be isolated from the main products of the fermentation and it did not disturb further degradation procedures. the methyl-c-atom of ethanol, which was derived from c-2 of the mannose, had nearly the same specific radioactivity as mannose-2-14c. all other c-atoms of the degradation products were on ... | 1975 | 1217941 |
| a comparison of the anaphylactoid actions of a synthetic linear dextran and a natural branched dextran. | synthetic linear dextran of molecular weight 40,000 produces the systemic anaphylactoid reaction in rats although it is about 4 times less active than the natural branched dextran of similar molecular weight. similarly, it is less active on local injection into the foot or skin. however, in rats which have been bred for non-reactivity to systemic dextran, it is more active on local injection, resembling the activity of a branched natural dextran of much lower molecular weight (6,000). in human s ... | 1976 | 1248893 |
| formation of acetaldehyde from threonine by lactic acid bacteria. | group n streptococci were found to cleave threonine to form acetaldehyde and glycine. threonine aldolase, the enzyme catalysing this reaction, was found in all strains except streptococcus cremoris z8, an organism which had been shown previously to have a nutritional requirement for glycine. the enzyme was strongly inhibited by glycine and cysteine. the inhibition showed characteristics of allosteric inhibition and was ph-dependent. inhibition by glycine, but not by cysteine, was highly specific ... | 1976 | 1262564 |
| mesosomes in leuconostoc mesenteroides. | | 1976 | 1265956 |
| dextran-mediated interbacterial aggregation between dextran-synthesizing streptococci and actinomyces viscosus. | streptococcus sanguis and streptococcus mutans bind to the surface of actinomyces viscosus, producing large microbial aggregates. aggregates form rapidly and are not easily dissociated by vigorous mixing. the binding is mediated by dextran. glucose-grown streptococci will not aggregate unless they are first mixed with high-molecular-weight dextran. aggregation is induced with dextrans isolated from leuconostoc, s. sanguis, or s. mutans. sucrose-grown streptococci will adhere to a. viscosus witho ... | 1976 | 1279004 |
| lysine-21 of leuconostoc mesenteroides glucose 6-phosphate dehydrogenase participates in substrate binding through charge-charge interaction. | leuconostoc mesenteroides glucose 6-phosphate dehydrogenase (g6pd) was isolated in high yield and purified to homogeneity from a newly constructed strain of escherichia coli which lacks its own glucose 6-phosphate dehydrogenase gene. lys-21 is one of two lysyl residues in the enzyme previously modified by the affinity labels pyridoxal 5'-phosphate and pyridoxal 5'-diphosphate-5'-adenosine, which are competitive inhibitors of the enzyme with respect to glucose 6-phosphate (ladine, j.r., carlow, d ... | 1992 | 1304341 |
| isolation and characterization of is1165, an insertion sequence of leuconostoc mesenteroides subsp. cremoris and other lactic acid bacteria. | we have cloned and characterized an insertion sequence from leuconostoc mesenteroides subsp. cremoris strain db1165. this element, designated is1165, is 1553 bp, has imperfect inverted repeat ends, contains an open reading frame of 1236 bp, and is not related to any previously described insertion sequence. the copy number of is1165 varies from 4 to 13 in l. mesenteroides subsp. cremoris strains allowing genetic fingerprinting of strains based on location and number of bands on hybridization. is1 ... | 1992 | 1325060 |
| systematics of the lactobacillus population on rat intestinal mucosa with special reference to lactobacillus reuteri. | the systematics of the lactobacillus population of the intestines of 88 different rats was studied; 80 rats had been fed on fermented oat-meal soup (molin et al. 1992). one-hundred-twenty-two lactobacillus strains from the intestinal mucosa were phenotypically classified together with twenty-eight reference strains of lactobacillus and leuconostoc, using 49 unit characters. data were examined using jaccard coefficient, and unweighted pair group algorithm with arithmetic averages. two major and e ... | 1992 | 1325752 |
| an enzymatic method for measuring serum mannitol and its use in hemodialysis patients. | we evaluated two chemical methods for quantifying mannitol in serum, based on the oxidation of mannitol by periodate, and measurement of the formaldehyde formed with chromotropic acid (colorimetry) or acetylacetone (fluorometry). we found interference in these methods by serum glycerol. additionally, a high-performance liquid chromatography (hplc) method was evaluated and found to be specific but impractical for routine use. we therefore, developed an enzymatic fluorometric procedure, based on t ... | 1992 | 1335850 |
| a rapid, quantitative microplate assay for nad-linked d-mannitol dehydrogenase. | a 96-well microtitre plate assay for nad-linked d-mannitol dehydrogenase based on the reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) by reduced nad is described. the assay allows rapid measurement of d-mannitol dehydrogenase in crude bacterial extracts derived by sonic disruption, in acetone permeabilized cells and in column eluates during enzyme purification. the absorbance of reaction mixtures in a microtitre plate is measured at 620 nm over a 3-4 min period us ... | 1990 | 1366858 |
| purification and some properties of sucrose phosphorylase from leuconostoc mesenteroides. | sucrose phosphorylase (ec 2.4.1.7) was purified to homogeneity from leuconostoc mesenteroides cells with a specific activity of 173.8 units per mg protein by ammonium sulfate fractionation, anion exchange hplc on tskgel deae-5pw, and hydrophobic hplc on tskgel ether-5pw. the purified enzyme was an acidic protein having an isoelectric point of ph 4.6 and s0(20),w of 4.34s. the molecular weight of this enzyme was estimated to be 56,400 by sedimentation equilibrium, 55,000 by sds-polyacrylamide gel ... | 1991 | 1368718 |
| description and evaluation of the semiautomated 4-hour rapid id 32 strep method for identification of streptococci and members of related genera. | the rapid id 32 strep system (biomérieux, la balme les grottes, france) is a new system which allows the identification in 4 h of most streptococci and members of related genera encountered in medical and veterinary bacteriology. four hundred thirty-three isolates first identified by conventional methods and belonging to the genera streptococcus, lactococcus, enterococcus, aerococcus, gemella, leuconostoc, erysipelothrix, gardnerella, and listeria were tested. overall, rapid id 32 strep correctl ... | 1992 | 1400965 |
| intracellular ph and the role of d-lactate dehydrogenase in the production of metabolic end products by leuconostoc lactis. | the kinetics of lactate dehydrogenase from leuconostoc lactis ncw1 were studied. the ph optimum for the enzyme depended on the concentration of pyruvate used in the assay and the enzyme displayed an ordered mechanism with respect to substrate binding. the km for pyruvate and nadh and the vmax of the enzyme decreased 20-, 30- and 6-fold respectively as the ph decreased from 8.0 to 5.0. no activators were found and none of the intermediates of the phosphoketolase pathway tested inhibited the enzym ... | 1992 | 1401357 |
| purification and amino acid sequence of a bacteriocin produced by pediococcus acidilactici. | a bacteriocin produced by pediococcus acidilactici has been purified to homogeneity by a rapid and simple four-step purification procedure which includes ammonium sulphate precipitation, chromatography with a cation-exchanger and octyl sepharose, and reverse-phase chromatography. the purification resulted in an approximately 80,000-fold increase in the specific activity and about a 6-fold increase in the total activity. the amino acid composition and sequencing data indicated that the bacterioci ... | 1992 | 1402795 |
| leuconostoc species bacteremia in a child with acquired immunodeficiency syndrome. | | 1992 | 1424402 |
| in vitro activity of mersacidin (m87-1551), an investigational peptide antibiotic tested against gram-positive bloodstream isolates. | we measured the in vitro activity of mersacidin (formerly m87-1551) against 183 clinical isolates (vancomycin susceptible) and 12 additional vancomycin-resistant strains of gram-positive bacteria. the activity for mersacidin increased an average twofold (range, 1.7- to 7.6-fold) in a calcium-enriched medium. the minimum inhibitory concentration (mic)90 for mersacidin was 8-32 times higher than vancomycin for staphylococci, 4-64 times higher for enterococci, and up to 32 times higher for other or ... | 1992 | 1424522 |
| identification and characterization of two bacteriocin-producing strains of lactococcus lactis isolated from vegetables. | isolated from mixed salad and fermented carrots, 123 strains of lactic acid bacteria were screened for bacteriocin production. two strains, d53 and 23, identified as lactococcus lactis by dna-dna hybridizations, produced heat stable bacteriocins which were resistant to trypsin and pepsin, but were inactivated by alpha-chymotrypsin and proteinase k. the bacteriocins were active from ph 2 to 9 and inhibited species of listeria, lactobacillus, lactococcus, pediococcus, leuconostoc, carnobacterium, ... | 1992 | 1445757 |
| the ability of the ropy slime-producing lactic acid bacteria to form ropy colonies on different culture media and at different incubation temperatures and atmospheres. | the ability of the ropy slime-producing lactic acid bacteria to form ropy colonies aerobically and anaerobically on apt, mrs, mrs-s and rogosa sl agars at 15, 20 and 30 degrees c was tested. wide variation was observed in the proportion of ropy colonies produced with different culture methods and different test periods. the percentage of viscous colonies was usually highest at 15 degrees c. no suitable uniform method for all strains was found; and the results varied according to the bacterial st ... | 1992 | 1445760 |
| classification of ropy slime-producing lactic acid bacteria based on dna-dna homology, and identification of lactobacillus sake and leuconostoc amelibiosum as dominant spoilage organisms in meat products. | the classification of lactic acid bacteria able to cause ropy slime on vacuum-packed cooked meat products was carried out based on dna-dna homology. the ropy slime-producing lactobacilli were identified as strains of lactobacillus sake and the ropy slime-producing leuconostocs, such as leuconostoc amelibiosum and leuconostoc mesenteroides. | 1992 | 1445761 |
| characterization and partial purification of a bacteriocin produced by leuconostoc carnosum la44a. | twenty leuconostoc strains isolated from vacuum packaged vienna-type sausages were screened for antagonistic activity against various gram-positive organisms (including listeria spp.). one of the three strains exhibiting inhibitory activity was chosen for further investigation. this strain was identified as leuc. carnosum and the inhibitory substance produced was named carnosin. carnosin was inactivated by trypsin but not by catalase or other non-proteolytic enzymes tested. carnosin retained act ... | 1992 | 1445765 |
| on-line visualization of the competitive behavior of antagonistic bacteria. | to study the interaction between cocultured listeria monocytogenes and an antagonistic leuconostoc strain producing an anti-listeria bacteriocin, flow cytometry, a technique allowing on-line and real-time analysis, was used along with classical microbiological methods. culture methods and flow cytometric measurements of the mixed culture over time point to a bactericidal action of the lactic acid-producing bacterial strain against l. monocytogenes cells. | 1992 | 1482199 |
| culture media for non-sporulating gram-positive food spoilage bacteria. | the spoilage association especially of protein-rich foods can be dominated by gram-positive bacteria, notably lactic acid bacteria (lab) which affect vacuum packaged refrigerated processed meats and some dairy products. new food ecosystems are being created by novel packaging and processing technologies, resulting in spoilage associations differing from those previously reported. in addition, improvement in identification methods, allow the detection and isolation of 'novel' bacterial groups, e. ... | 1992 | 1486021 |
| characterization and purification of mesentericin y105, an anti-listeria bacteriocin from leuconostoc mesenteroides. | a leuconostoc mesenteroides ssp. mesenteroides was isolated from goat's milk on the basis of its ability to inhibit the growth of listeria monocytogenes. the antimicrobial effect was due to the presence in the culture medium of a compound, named mesentericin y105, excreted by the leuconostoc mesenteroides y105. the compound displayed known features of bacteriocins from lactic acid bacteria. it appeared as a proteinaceous molecule exhibiting a narrow inhibitory spectrum limited to genus listeria. ... | 1992 | 1487737 |
| denaturation of glucose-6-phosphate dehydrogenase from leuconostoc mesenteroides by guanidine hydrochloride; identification of inactive, partially unfolded, dimeric intermediates. | the denaturation of the dimeric enzyme glucose-6-phosphate dehydrogenase from leuconostoc mesenteroides by guanidine hydrochloride has been studied using enzymatic activity, intrinsic fluorescence, circular dichroism, and light scattering measurements. equilibrium experiments at 25 degrees c revealed that between 0.9 and 1.2 m denaturant the enzyme underwent a conformational change, exposing tryptophan residues to solvent, with some loss of secondary structure and a complete loss of enzymatic ac ... | 1992 | 1504085 |
| in vitro activity of teicoplanin and vancomycin against gram-positive bacteria from human clinical and veterinary sources. | the minimum inhibitory concentration (mic) of teicoplanin and vancomycin was determined by the agar dilution method for 186 gram-positive bacteria from human clinical and veterinary sources. teicoplanin mic values were less than or equal to 4 micrograms/ml for 94% of staphylococci (group a, n = 52) and less than or equal to 2 micrograms/ml for all streptococci, enterococci, aerococci and pediococci (group b, n = 75). seventy-eight percent of gram-positive rods, rhodococcus and leuconostoc spp. ( ... | 1992 | 1535201 |
| modification of histidine residues in proteins by reaction with 4-hydroxynonenal. | we find that histidine residues in proteins are major targets for reaction with the lipid peroxidation product 4-hydroxynon-2-enal (hne). reaction of insulin (which contains no sulfhydryl groups) with hne leads to the generation of hne-protein adducts, which are converted to radioactive derivatives upon subsequent treatment with nab[3h]h4. amino acid analysis of the modified protein showed that the hne treatment leads to the selective loss of histidine residues and the stiochiometric formation o ... | 1992 | 1584790 |
| effect of sodium nitrite and sodium chloride on growth of lactic acid bacteria. | the effect of nano2 and nacl on the growth of 24 lactic acid bacteria strains isolated from vacuum-packed cooked ring sausages were examined by analyzing different growth parameters with bioscreen. nano2 had a very limited effect on the growth of lactic acid bacteria at 50 and 100 mg/l but at 400 mg/l a more pronounced inhibitory effect was found. bacterial growth was enhanced by 1-2% (w/v) of added nacl, while nacl concentrations above 3% (w/v) had a clear inhibitory effect. leuconostoc isolate ... | 1992 | 1598855 |
| novel paired starter culture system for sauerkraut, consisting of a nisin-resistant leuconostoc mesenteroides strain and a nisin-producing lactococcus lactis strain. | nisin-resistant leuconostoc mesenteroides nck293 and nisin-producing lactococcus lactis subsp. lactis nck401 were evaluated separately and in combination for growth and nisin production in a model sauerkraut fermentation. strains were genetically marked and selectively enumerated by using antibiotic-containing media. the growth and survival of l. mesenteroides were similar in the presence and absence of lactococcus lactis subsp. lactis. the growth of lactococcus lactis subsp. lactis was not inhi ... | 1992 | 1622215 |
| chemical, microbial and sensory changes during the anaerobic cold storage of beef inoculated with a homofermentative lactobacillus sp. or a leuconostoc sp. | slices of beef were inoculated with about 3.5 log cfu/cm2 of lactobacillus sp. 93 smricc 235 (homofermentative) or leuconostoc sp. 89 smricc 189 and stored in 5% co2 + 95% n2 at 4 degrees c. the microbial, chemical (glucose, l-lactate, d-lactate, acetate, formate, ethanol, h2s) and sensory changes of the beef slices were studied. for beef inoculated with lactobacillus sp. 93 the flavour score started to decrease when the maximum bacterial count was reached. leuconostoc sp. 89 caused a rapid decr ... | 1992 | 1622763 |
| leuconostoc lactis beta-galactosidase is encoded by two overlapping genes. | a 16-kb bamhi fragment of the lactose plasmid pnz63 from leuconostoc lactis nz6009 was cloned in escherichia coli mc1061 by using pacyc184 and was found to express a functional beta-galactosidase. deletion and complementation analysis showed that the coding region for beta-galactosidase was located on a 5.8-kb sali-bamhi fragment. nucleotide sequence analysis demonstrated that this fragment contained two partially overlapping genes, lacl (1,878 bp) and lacm (963 bp), that could encode proteins w ... | 1992 | 1624440 |