| biochemical differentiation of aggregating cell cultures of different fetal rat brain regions. | rotation-mediated aggregating cell cultures of mechanically dissociated fetal rat brains divided into three (telencephalon, mesencephalon-diencephalon and rhombencephalon), or two (telencephalon and mesencephalon-diencephalon plus rhombencephalon) parts were examined for their biochemical differentiation by measuring the specific activities of choline acetyltransferase, acetylcholinesterase, glutamic acid decarboxylase, tyrosine 3-monooxygenase, aromatic l-amino acid decarboxylase, catechol meth ... | 1977 | 20195 |
| confirmation of conductance increase by adrenalin in the guinea-pig taenia coli (alpha-action). | | 1977 | 21402 |
| an adenosine 3':5'-monophosphate-adenosine binding protein from mouse liver. factors affecting the activation of the binding protein by adenosine 5'-triphosphate. | | 1978 | 23726 |
| isolation and characterization of the acidic phosphoproteins of 60-s ribosomes from artemia salina and rat liver. | eucaryotic l7/l12-type proteins are present in ethanol/salt extracts (p1 protein) of ribosomes from artemia salina and rat liver. these proteins are partially phosphorylated and occur in two forms of closely related structure: a major form el12 having methionine at the n-terminal position and a minor form of el12 (el12') which seems slightly elongated and contains a blocked n terminus. purification of the four different forms of this protein, el12, el12-p, el12' and el12'-p, was performed by ion ... | 1978 | 32034 |
| effect of natural variations in paco2 on plasma [hco3-] in dogs: a redefinition of normal. | graded degrees of both chronic hyper- and hypocapnia are known to induce renal responses that significantly alter plasma bicarbonate concentration. these findings have raised the possibility that even normal variations in paco2 play an important role in determining the exact level of bicarbonate in plasma. to test this hypothesis, we examined the relationship between resting levels of paco2 and the plasma bicarbonate concentration in two groups of normal dogs, one ingesting a normal salt diet an ... | 1979 | 35008 |
| stoichiometry of h+ translocation coupled to electron flow from succinate to cytochrome c in mitochondria. | | 1979 | 40984 |
| slow encephalopathies, inflammatory responses and arachis oil. | there is much interest in the possibility that diets rich in some plant oils may be of prophylactic and curative value in certain clinical conditions. this is generally attributed to the immunosuppressive effects of polyunsaturated fatty acids. arachis (peanut) oil injections can increase the incubation period of experimental scrapie in mice. in the late 1950s several workers showed that arachis and some other oils contained very potent anti-inflammatory components quite apart from the polyunsat ... | 1975 | 47424 |
| alpha2-macroglobulin on human vascular endothelium. | alpha2-macroglobulin (alpha2m) has been identified on the luminal surface of endothelial cells in sections of normal human arteries, veins, and lymphatics by the indirect immunofluorescent technique. the specificity of the immunofluorescent reaction was confirmed by immunoabsorption studies. prior absorption of the anti-alpha2m antiserum by purified alpha2m at equivalence completely inhibited endothelial surface as well as hepatic parenchymal cell staining. endothelial cells in blood vessels wer ... | 1976 | 58955 |
| the use of ionophores of rapid loading of human red cells with radioactive cations for cation-pump studies. | techniques are described for the rapid loading of intact human red cells with radioactive isotopes of alkali cations or ca2+ by using ionophorous compounds (nigericin, gramicidin d and a 23187). loading was rapid and efficient if the membrane potential of the cells was rendered more negative inside. after cation loading the ionophores could be bound to albumin and removed by repeated washings. the atp and 2,3-dpg contents of the cells were practically unaltered by this treatment. passive membran ... | 1976 | 58995 |
| cultivation of viral agents from crohn's disease. a new sensitive system. | the isolation and animal transmission of a viral agent from crohn's disease patients stimulated studies aimed at the development of improved tissue culture techniques. the need for an effective tissue-culture system led to a comparison of african green monkey tissue-culture cells (a.g.m.k.), human diploid lung cells (wi-38), and a new tissue-culture line--continuous rabbit ileum (c.r.i.). homogenates prepared from ileal specimens from four crohn's disease patients and from four control patients ... | 1976 | 59237 |
| letter: macrophages v. cancer. | | 1976 | 59265 |
| response to hormones of cells cultured from human giant cell tumors of bone. | giant cell tumors of bone obtained from 7 patients were dispersed with clostridial collagenase and trypsin and adherent cells were maintained in culture. early cultures contained both mononucleated and multinucleated cells presumably derived from the stromal and giant cells of the original tumor. the original multinucleated cells did not survive for greater than 7-10 days whereas the mononucleated cells persisted and could be passaged by trypsinization. in 5 of 7 early cultures exposed to parath ... | 1978 | 87400 |
| decreased axonal flux of retrogradely transported glycoproteins in early experimental diabetes. | | 1979 | 91664 |
| [antigenic structure of the l forms of br. abortus 544]. | the gel precipitation test after ouchterlony and mancini was employed to study comparatively the antigenic structures of s and l forms of brucella abortus 544. reduction of the antigenic fractions was established with the l-transformed brucella organisms as compared to the initial cultures. reduced were mainly fractions of the antigenic structures that took part in cell wall building. cytoplasmic antigens remained unchanged. those that participated in the building of cell walls precipitated in c ... | 1979 | 92852 |
| relatedness among coagulase-negative staphylococci: deoxyribonucleic acid reassociation and comparative immunological studies. | dna-dna-homology values were determined under restrictive to relaxed reassociation conditions with type strains and some additional strains of coagulase-negative staphylococci belonging to ten different species. the immunological relationship of the catalases present in the type strains of these species was also determined by applying double immunodiffusion and microcomplement fixation. the results of these studies support the previous proposal to subdivide the coagulase-negative staphylococci i ... | 1979 | 92972 |
| [two-layer rocket-electrophoresis on cellulose-acetate membranes]. | two-floor rocket-electrophoresis on gelatinated acetate cellulose membrane "cellogel" has been developed. the method is based on electroimmunodiffusion detection of the antigen on the acetate-cellulose membranes, containing monospecific antiserum of the test-system. the procedure is followed by the detection of precipitation bands ("rockets") by staining, if the reaction is conducted in the visible zone or by the further treatment of the acetate-cellulose strips, containing invisible precipitate ... | 1979 | 93004 |
| immunological properties of peptic fragments of bovine serum albumin. | the effect of peptic degradation on the immunological and antigenic properties of bovine serum albumin (bsa) was investigated. molecular fragments obtained after various times of digestion (3-360 min) were studied. enzymatic digestion resulted in a rapid loss of serologically defined antigenic determinants. the immunogenicity of the fragments as measured by the level of reaginic and total anti-bsa antibody response in bdf1 mice was also diminished. pre-treatment of mice with fragments exhibiting ... | 1979 | 93079 |
| occurrence and subcellular localization of glucose 6-phosphatase in bovine thyroid. | in bovine thyroid tissue the glucose 6-phosphatase activity is not entirely due to the presence of an unspecific acid phenylphosphatase nor to beta-glycerophosphatase. this glucose 6-phosphatase is very probably localized within endoplasmic reticulum membranes. it is not a good marker for distribution patterns obtained after differential pelleting. however it can be used as a marker for endoplasmic reticulum membranes after centrifugation in a zonal rotor. | 1979 | 93889 |
| exposed sulphydryl groups of types of haemoglobin during human ontogenesis. | | 1978 | 94245 |
| antigenic components of taenia saginata and their relevance to the diagnosis of bovine cysticercosis by immunoelectrophoresis. | 22 antigenic components of taenia saginata proglottides were detected by immunoelectrophoretic (iep) study. cross-absorption of the hyperimmune serum, raised in rabbits against t. saginata, with heterologous and host antigens showed that two thirds of these components were not specific for the cestode. among the calves experimentally infected with t. saginata eggs, 3 precipitation patterns in iep were identified depending on the evolutionary stage of the infection. the hydrosoluble extract of t. ... | 1979 | 94334 |
| macrophage-lymphocyte clusters in the immune response to soluble protein antigen in vitro. viii. cinephotomicrographic studies. | t cells from immune guinea-pigs produce clusters in vitro with macrophages pulsed with soluble protein antigens. the formation of clusters is antigen-specific. time-lapse cinephotomicrography was used to study the sequence of events leading to cluster formation and the kinetics of already formed clusters. the central lymphocyte attaches through a broad base to the macrophage during the first few hours of incubation. after several hours, during which the central lymphocyte is situated alone on th ... | 1979 | 94460 |
| dependence of macromolecular composition and morphology of streptomyces hygroscopicus on specific growth rate. | the dependence of macromolecular composition and morphology of streptomyces hygroscopicus on specific growth rate micron was investigated. the percentage of dna on dry weight (%dna) is constant, % protein is also nearly independent of micron whereas %rna rises considerably with increasing micron, regarding mycelia grown in glucose-limited and ammonium-limited continuous cultures as well as in discontinuous cultures with various carbon sources. it is probable that the overall synthesis of dna, rn ... | 1979 | 94484 |
| a detergent-citric acid technique for isolating nuclear and cytoplasmic fractions containing undegraded rna from cells of xenopus laevis. | | 1979 | 94513 |
| pyrazolidine derivatives: a comparative study of their effects on platelet aggregation. | quantitative studies were carried out of the in vitro and ex vivo effects of phenylbutazone and 3-oxoalkyl substituted diphenyldioxopyrazolidines (kebuzone, tribuzone, benzopyrazone) on platelet aggregation. the specified pyrazolidine derivatives exhibited in vitro inhibitory effects on secondary platelet aggregation (induced by adrenaline and collagen), commensurable with the effects of sulfinpyrazone. the ex vivo efficacy was markedly influenced by the height of the drug level in blood and by ... | 1979 | 94876 |
| application of uracil-bonded silica gel to the separation of adenine and its derivatives, and to poly(a)-containing rna. | | 1979 | 94919 |
| [influence of parotin on the repressing action of cyanylethyl group to histamine stress (author's transl)]. | | 1978 | 95057 |
| roles of peptides in central transmission: substance p, bradykinin and b-endorphin. | | 1979 | 95614 |
| isolation of the l-phase variant from toxigenic corynebacterium diphtheriae c7(beta). | the l-phase variant was isolated from lysogenic strain c7(beta) of corynebacterium diphtheriae by the penicillin disk tecnnique on an agar medium containing 3.7% brain heart infusion, 3% nacl, 1.25% grycine, 1% noble agar, and 10% horse serum. the l-phase formed a "fried egg" colony; it was resistant to antibiotics that inhibit biosynthesis of bacterial cell walls but was susceptible to antibiotics that act on the protein-synthesizing system. after several transfers of the l-phase initially isol ... | 1978 | 97222 |
| [experience with the use of the coulter counter model "s" in the field of animal experimentation]. | | 1978 | 98930 |
| pharmacological studies of nitroharmidine nitrate on central nervous system. | | 1978 | 99533 |
| ineffectiveness of prophylactic intrathecal methylprednisolone in myelography with aqueous media. | the effectiveness of prophylactic intrathecal methylprednisone (mp) in preventing arachnoiditis from iocarmate myelography was studied. monkeys were injected intrathecally with locarmate, mp, or a combination of the two. twelve weeks later the severity of arachnoiditis was determined in each animal. there was no significant prophylactic effect of intrathecal mp on arachnoiditis from locarmate. some arachnoiditis was found in control animals treated with mp alone. | 1978 | 99764 |
| congeners of dom: effect of distribution of the evaluation of pharmacologic data. | | 1978 | 101880 |
| a new sequence-specific endonuclease from anabaena cylindrica. | | 1978 | 103749 |
| primary meningococcal conjunctivitis in an infant. | a case of primary meningococcal conjunctivitis in an infant was presented and its treatment is discussed. because of the potential for systemic spread of the infection in the patient, local and systemic therapy was instituted. the current literature on meningococcal conjunctivitis with its potential complications is briefly reviewed. | 1979 | 106998 |
| [effect of amikacin on experimental tuberculosis of mice (author's transl)]. | | 1979 | 107361 |
| [nuclei of the mammillary bodies and their fiber connections in cattle (bos taurus var. domesticus). i. nuclei of the mammillary bodies in cattle]. | 1. in the present neuroanatomic study the topography and cytoarchitectonic of the nuclei of the mamillary body of cattle are described and quantitative-morphologically investigated in 14 series of brains (6 frontal, 4 sagittal and 4 horizontal series, stained by the methods after nissl, klüver and barrera and after heidenhain). 2. the medial mamillary nucleus of cattle is with an average volume of 47.7 mm3 the largest nucleus of the mamillary body. it contains about 715,000 cells with a cell den ... | 1978 | 107664 |
| human carotid artery diameter and flow by a noninvasive technique. | this paper presents a technique tested in vitro and in dogs and used in humans to measure carotid artery blood flow continuously by placing a transducer on the skin over the artery. the tranducer consists of a pulse echo crystal used to locate the carotid artery and measure its diameter and wall velocity. it also has a pair of doppler shift crystals used to measure the velocity of the artery wall and blood flow in the vessel. it was found that the pulsatile artery wall distension was between 8 a ... | 1975 | 124007 |
| [radioimmunoassay without extraction of l-triiodothyronine in human serum]. | a radio-immunoassay of l-triiodothyronin is described. blinding of t3 to tbg is prevented by salicylate (1%--w/v). the rabbit antiserum obtained by injection of a complexe t3-bovine serum albumin is diluted between 1/10000 and 1/20000. bound and free hormone are separated by polyethylene glycol (17%--w/v). serum t3-concentration in normal subjects averaged 132 +/- 29 ng/dl (sd). in hyperthyroid and hypothyroid patients, these values were respectively 351 +/- 118 ng/dl and 68 +/- 21 ng/dl. | 1975 | 126769 |
| [study of the incorporation of radioactive zinc in saccharomyces cerevisiae aldolase]. | saccharomyces cerevisiae aldolase concentrates from the culture medium containing znso4 a large amount of zn which becomes a component part of the enzyme molecular structure. this was made evident by adding to the culture medium 65znso4 and measuring the radioactivity of the aldolase extracted by a phillips single channel analyzer. | 1976 | 133445 |
| the influence of age on the wound healing of experimental myocardial infarction in rats. | in 6-weeks-old, 5-months-old and 21-months-old rats myocardial infarction was induced by coronary artery ligature. after performing the ligature the animals were administered 3h-thymidine, 3h-proline or 35s-sulphate at different times. the following parameters were determined: number of dna- and tropocollagen-synthesizing connective tissue cells at the infarction border and at the infarction site; mean silver grain density above the nuclei or cells; duration of a cycle; number of mitoses, and in ... | 1975 | 134906 |
| [use of intraspecific differentiation of sh. sonnei for the purpose of studying patterns in the epidemic process of dysentery]. | the use of complex typing of sh. sonnei by the colicinogenic and enzymatic properties in epidemiological analysis of dysentery caused by sh. sonnei, apart from establishment of epidemiological connections in the foci, offered a possibility of introducing significant corrections in the determination of the sizes and duration of existence of the epidemic foci, as well as in detection of the sources and factors of transmission of the infection established in epidemiological examination. typing of s ... | 1978 | 154276 |
| the effect of beef spleen cathepsin d on pig heart lactate dehydrogenase. | | 1975 | 166614 |
| ultrastructural demonstration of phosphatases by perfusion fixation followed by perfusion incubation of rat liver. | an alternative to previous methods (tissue chopper, frozen sections) for the ultrastructural demonstration of phosphatases is described. the present approach is based on a short vascular perfusion of rat liver with glutaraldehyde through the inferior caval vein, followed by vascular perfusion incubation with a medium containing the enzyme substrates. the effect of glutaraldehyde on three different types of phosphatases was investigated, namely a lysosomal enzyme (acid phosphatase) a tightly boun ... | 1975 | 166967 |
| pigmentation and dysfunction of gunn rat thyroid: correlation between morphological and biochemical data. | the thyroid gland of homozygous gunn rats is moderately enlarged and displays a brownish-black discoloration. light microscopic examination discloses that the follicular cells are filled with brown granules, which are shown, under the electron microscope, to be modified colloid droplets. most of them possess a strong acid phosphatase and a mild peroxidase activity and contain a melanin-like pigment, according to histochemical analysis. in comparison with normal wistar rats, gunn rats possess sig ... | 1976 | 176774 |
| studies on the pituitary "fettstoffwechselhormon". vi. preparation of two highly purified lipolytic active peptides from hog pituitary glands. | the preparation of two highly purified lipolytically active hog pituitary peptides, called p-lf ii c and p-lf ii d is described. the two peptides are free of other pituitary hormone activities. in isolated rat and porcine adipose tissue, both fractions are lipolytically much more active than every other lipolytic active pituitary peptide described to date. by fraction p-lf ii d, the first pituitary peptide was isolated which has lipolytic activity in isolated rat adipose tissue than corticotropi ... | 1976 | 181302 |
| type-c rna viruses and leukemogenesis: relation of type-c virus infectivity and leukemogenesis induced by nitrosourea compounds in mice. | correlation between infectivity of type-c rna virus) murine leukemia virus, mlv) and development of leukemia was tested in female icr/jcl mice treated with either 1-ethyl-1-nitrosourea (enu) or 1-butyl-1-nitrosourea (bnu). continuous administration of either chemicals resulted in the occurrence of thymic lymphoma in every mouse with a short latent period. the time of appearance and distribution pattern of mlv infectivity in various tissues were examined by the xc plaque assay technique at fixed ... | 1976 | 187521 |
| [studies on the typing of a foot-and-mouth disease strain (type a)]. | | 1978 | 208502 |
| effect of galactose on free radical reactions of polymorphonuclear leukocytes. | | 1978 | 209746 |
| isolation of the proviral coding strand of avian myeloblastosis virus from leukemic chicken myeloblast by affinity chromatography. | | 1978 | 210689 |
| a rapid and sensitive fluorescence assay for angiotensin-converting enzyme. | | 1978 | 210690 |
| preparative isoelectric focusing of human serum very low-density and low-density lipoproteins. | | 1978 | 211872 |
| inhibition of protein synthesis by glucose 6-phosphate and fructose 1,6-diphosphate in lysed rabbit reticulocytes and the reversal of inhibition by nad+. | | 1978 | 212031 |
| characterization of nadh-cytochrome c reductase, a component of benzoate 1,2-dioxygenase system from pseudomonas arvilla c-1. | nadh-cytochrome c reductase, a component of benzoate 1,2-dioxygenase system, was purified to homogeneity, as judged by sodium dodecyl sulfate disc gel electrophoresis and ultracentrifugation, from benzoate-induced cells of pseudomonas arvilla. the molecular weight of the enzyme was determined to be 38,300 by sedimentation equilibrium analysis, 37,000 by sephadex g-100 gel filtration, and 37,500 by sodium dodecyl sulfate disc gel electrophoresis, respectively, indicating that the enzyme consisted ... | 1978 | 214433 |
| clinical, neuropathologic and pharmacologic aspects of huntington's disease: correlates with a new animal model. | | 1977 | 214805 |
| ontogenesis of 3,5,3'-triiodothyronine receptors in neonatal rat brain: dissociation between receptor concentration and stimulation of oxygen consumption by 3,5,3'-triiodothyronine. | | 1978 | 217661 |
| transport atpase--the different modes of inhibition of the enzyme by various mercury compounds. | | 1979 | 218593 |
| flavonoids are selective cyclic gmp phosphodiesterase inhibitors. | | 1979 | 218594 |
| regulation of the surface physical properties of the very low density lipoprotein. | | 1979 | 218937 |
| [stress and immunity in cattle]. | stress of variable length may be caused in calf by parenteral administration of supercortisol and application of acute thermal load. hormonal or thermal stress may have negative impact on postvaccinal immunity to paratyphoid (salmonella dublin 81), as may be interpreted with reference to the o-agglutination titre of blood serum. the extent to which immunity is suppressed by stress was found to depend on the moment of stress application, duration of stress, and age of the calf. | 1979 | 222226 |
| infection studies on a reticuloendotheliosis virus contaminant of a commercial marek's disease vaccine. | reticuloendotheliosis virus (rev) was isolated in cell cultures from commercial marek's disease (herpesvirus of turkeys) vaccine and re-isolated from the organs of vaccinated chickens. runting and feathering abnormalities were produced when 1-day-old specific pathogen free chickens were inoculated with rev. histopathological lesions in infected chickens were hypoplasia of the thymus, bursa and spleen, and inflammation of the proventriculus, kidneys and liver. serological responses to rev were de ... | 1979 | 223539 |
| [demonstration of the oncogenic activity of cell cultures using antithymocytic serum]. | the authors examined 10 cell strains of different origin as to their effect on mice by means of antithymocytic (atc) serum. in dependence on the strain used the tumors developed in different number and with different growth tendency. in control animals not treated by the atc serum, small ganglions developed in some cases which, however, disappeared in 2--3 days. both strains of diploide cells wi 38 and lep and primary cultures of chicken fibroblast from embryos of spf chickens did not develop an ... | 1978 | 311797 |
| atp induces nucleotide permeability in rat mast cells. | | 1979 | 450099 |
| equilibrium saturation chromatographic method for studying the binding of ligands to human serum albumin by high-performance liquid chromatography. influence of fatty acids and sodium dodecyl sulphate on warfarin-human serum albumin binding. | a size exclusion chromatographic method for studying ligand-macromolecule binding parameters is described. this equilibrium saturation method allows the determination of the concentrations of constituents in equilibrium and is specially useful for characterizing ligand--protein binding under conditions that can be compared with physiological conditions. the method has been used for measuring warfarin--human serum albumin (hsa) binding and for studying the influence of free fatty acids (ffa) and ... | 1979 | 541445 |
| isolation of bovine brain microtubule protein without glycerol: polymerization kinetics change during purification cycles. | | 1979 | 543557 |
| histological differences in the epithelium of denture-bearing and non-denture-bearing human palatal mucosa. | | 1975 | 1054567 |
| the implantation of human dentin and cementum and freshly devitalized autogenous bone into the subcutaneous tissues of the rat. | | 1975 | 1055795 |
| radionuclide imaging of hard and soft tissues of dog jaws under heavy orthodontic forces. | rectilinear scanning with soft tissue, vascular and skeletal imaging agents was used to study the effects of heavy orthodontic forces on the tissues of dog jaws. a coil delivering either a 300 or 600 gram separating force to two adjacent lower right posterior teeth was inserted in each of four dogs. the animals were scanned with the three imaging agents three to four week after insertion of the coils. only the skeletal agent, tc-pp, was selectively concentrated in tissues adjacent to the stresse ... | 1976 | 1066979 |
| [thymus hormones]. | | 1975 | 1080268 |
| [march hemoglobinuria. one case with erythrocyte glutathione peroxidase deficiency]. | a new case of march haemoglobinuria seen in a 21-year-old man is reported. a deficiency in two erythrocytic enzymes (glutathione reductase and glutathione peroxydase) was observed. treatment with riboflavin corrected the glutathione reductase deficiency. the march haemoglobinuria and glutathione peroxydase deficiency persisted. several months after resolution of the march haemoglobinuria, a new estimation of glutathione peroxydase showed that the deficiency had disappeared. the possible role of ... | 1975 | 1134932 |
| regional distribution of monoamine oxidase activity for 5-hydroxytryptamine and tyramine in hypothalamus of the rat. | | 1975 | 1174989 |
| oxidation and metabolism of linoleic acid in the sheep. | | 1975 | 1187648 |
| proceedings: the uptake of adenine by isolated human platelet membranes. | | 1975 | 1198511 |
| [repeated obtaining of dog peritoneal macrophages and their cultivation in vitro]. | | 1975 | 1214674 |
| [test of degranulation of basophils and mast cells for detection of allergic diseases (literature survey)]. | | 1976 | 1274295 |
| stimulation of rat pancreatic tumoral ar4-2j cell proliferation by pituitary adenylate cyclase-activating peptide. | in the present work the effects of the novel neuropeptide pituitary adenylate cyclase activating peptide (pacap) on both ar4-2j cell growth and the modulation of ornithine decarboxylase activity were investigated. both pacap38 and the amidated form pacap27 caused a concentration-dependent stimulation of ar4-2j cell growth; the maximal increase was seen at 1 nmol/l (30% above control, p less than 0.01) with a half-maximal effect at 0.01 nmol/l. ornithine decarboxylase activity was also increased ... | 1992 | 1323494 |
| disparate effects of calcium channel blockers on pressure dependence of renin secretion and flow in the isolated perfused rat kidney. | using the model of isolated perfused rat kidneys this study was performed to investigate whether or not voltage-operated calcium channels are essentially involved in the pressure control of renin secretion from the kidneys. at a perfusion pressure of 100 mm hg (13.3 kpa) renin secretory rates were 4.2 +/- 0.7 (ng angiotensin i h-1) min-1 g-1. stepwise reduction of renal perfusion pressure to 80, 60, and 40 mm hg (10.6, 8.0, 5.3 kpa) resulted in an increase of renin release yielding a 30-fold sti ... | 1992 | 1382264 |
| [effect of biogenic amines on glycogen degradation in isolated rat hepatocytes]. | serotonin and tyramine affected dissimilarly the enzyme activity involved in degradation of glycogen in isolated rat hepatocytes. serotonin inhibited hydrolytic enzymes acid alpha-glucosidase, alpha-amylase and amylo-1,6-glucosidase but activated phosphorylase inducing a decrease of glycogen content in cells. tyramine inhibited acid alpha-glucosidase and amylo-1,6-glucosidase, did not affect the alpha-amylase and phosphorylase activities and increased content of glycogen in cells. tyramine may b ... | 1992 | 1413626 |
| examination of the local lymph node assay for use in contact sensitization risk assessment. | the purpose of this study was to evaluate the utility of the murine local lymph node assay (llna) for contact sensitization risk assessment. cellular proliferative activity in draining lymph nodes was determined for individual animals on day 5 following four daily epicutaneous applications of the test chemical to the ears. seventeen chemicals were tested, covering a range of materials including preservatives, drug actives, and perfume raw materials. the assay was found to be useful for identifyi ... | 1992 | 1459374 |
| synthesis and secretion of hirudin by streptomyces lividans. | to examine the secretory production of heterologous proteins by streptomyces lividans, we fused the dna encoding the signal peptide of the alpha-amylase inhibitor tendamistat, derived from s. tendae with a synthetic gene encoding the thrombin inhibitor hirudin. the analysis of secretion by immunoblots revealed an efficient translocation of hirudin through the membrane, with no detectable immunoreaction among the cellular proteins. the secreted hirudin was stable in the shaking culture for about ... | 1990 | 1367034 |
| synthesis and secretion of hirudin by streptomyces lividans. | to examine the secretory production of heterologous proteins by streptomyces lividans, we fused the dna encoding the signal peptide of the alpha-amylase inhibitor tendamistat, derived from s. tendae with a synthetic gene encoding the thrombin inhibitor hirudin. the analysis of secretion by immunoblots revealed an efficient translocation of hirudin through the membrane, with no detectable immunoreaction among the cellular proteins. the secreted hirudin was stable in the shaking culture for about ... | 1990 | 1367034 |
| acupuncture analgesia. | acupuncture-produced surgical analgesia has been demonstrated in a wide variety of species under experimental and clinical circumstances. the main advantage is that no depressant drugs need to be used. the disadvantages of its use are unfamiliarity, the need for special equipment, inconsistent effects, and lack of restraint. | 1992 | 1533972 |
| diagnosis of sickle cell disease in chronically transfused patients. | standard electrophoretic methods for the diagnosis of hemoglobinopathies are confounded in individuals chronically transfused. we present the accurate diagnosis of sickle cell disease in two such transfused patients by the application of polymerase chain reaction technology to analyze patient's hemoglobin beta-chain genes directly. | 1992 | 1550267 |
| infection of macaca nemestrina by human immunodeficiency virus type-1. | after observations that macaca nemestrina were exceptionally susceptible to simian immunodeficiency virus and human immunodeficiency virus type-2 (hiv-2), studies of hiv-1 replication were initiated. several strains of hiv-1, including a recent patient isolate, replicated in vitro in peripheral blood mononuclear cells (pbmcs) and in cd4-positive m. nemestrina lymphocytes in a cd4-dependent fashion. eight animals were subsequently inoculated with either cell-associated or cell-free suspensions of ... | 1992 | 1621083 |
| inhibition of nitric oxide synthase blocks n-methyl-d-aspartate-, quisqualate-, kainate-, harmaline-, and pentylenetetrazole-dependent increases in cerebellar cyclic gmp in vivo. | the synthesis of nitric oxide by brain slices has been demonstrated in several laboratories. in addition, in vitro studies have demonstrated stimulation of nitric oxide synthesis by excitatory amino acid receptor agonists. these data have led to the hypothesis that this readily diffusible "intercellular messenger molecule" acts to generate a cascade effect by activating guanylate cyclase in several cell types and thereby augment levels of the second messenger cyclic gmp (cgmp). therefore, we eva ... | 1990 | 1693947 |
| maintenance therapy for cytomegalovirus retinitis in patients with acquired immunodeficiency syndrome: foscarnet. | the use of ganciclovir in the treatment of cytomegalovirus (cmv) retinitis in patients with acquired immunodeficiency syndrome (aids) is limited by marrow toxicity and by the development of resistance to this agent in cmv strains capable of causing progressive disease. foscarnet retains activity against ganciclovir-resistant cmv and has an adverse effect profile different from that of ganciclovir. preliminary data from studies conducted under the aids clinical trials group (actg) program indicat ... | 1992 | 1310573 |
| measurement of the human immune response to meningococcal lipooligosaccharide antigens by using serum to inhibit monoclonal antibody binding to purified lipooligosaccharide. | we developed a human inhibition monoclonal enzyme-linked immunosorbent assay (himelisa) to investigate the human immune response to the lipooligosaccharides (los) of neisseria meningitidis. monoclonal antibodies (mab) were used to define seven epitopes on four los molecules of a meningococcal strain (126e) previously shown to express immunogenic los epitopes. the assay could distinguish epitope-specific antibody within whole sera. neither the specificity nor the amount of the antibody measured b ... | 1990 | 1694820 |
| load dependency and reliability of microhardness measurements on acid-etched enamel surfaces. | the load dependency of hardness measurements was investigated on sound and demineralized enamel samples. for all samples investigated, the knoop hardness number (khn) varied as a function of the applied load. higher loads enhanced indenter penetration into the test surface. the variation in khn values observed in demineralized enamel, both surface-softened and surface-etched, was explained by a decrease in porosity as a function of depth. the reading error for indentations made on surface enamel ... | 1992 | 1303377 |
| allergy to rabbits. iii. further identification and characterisation of rabbit allergens. | crossed immunoelectrophoresis and crossed radio-immunoelectrophoresis techniques have been employed for analysis of extracts of various rabbit source materials to identify rabbit allergens in addition to the already described antigen r1 (agr1). urine and dander extracts were found to contain only low levels of agr1 and its presence in urine was as a contaminant due to mode of collection--it was not present in urine collected directly from the bladder. other allergens were only recognised by high ... | 1991 | 1796773 |
| inhibitory effect of cimetidine on graft survival of allotransplanted rats submitted to an active-passive enhancement protocol. | the objective of the present study was to determine whether cimetidine, a type-2 histamine receptor antagonist, inhibits the immunological enhancement of allografted rats achieved by treatment with donor antigen plus anti-donor antibody. groups of rats submitted to this active-passive enhancement protocol and treated ip with 30 (apec 30; group i; n = 4) or 60 (apec 60; group ii; n = 8) mg/day cimetidine for 14 days had a significantly shorter graft survival (20.2 +/- 5.1 and 11.1 +/- 2.6 days, r ... | 1991 | 1797288 |
| inhibitory effect of cimetidine on graft survival of allotransplanted rats submitted to an active-passive enhancement protocol. | the objective of the present study was to determine whether cimetidine, a type-2 histamine receptor antagonist, inhibits the immunological enhancement of allografted rats achieved by treatment with donor antigen plus anti-donor antibody. groups of rats submitted to this active-passive enhancement protocol and treated ip with 30 (apec 30; group i; n = 4) or 60 (apec 60; group ii; n = 8) mg/day cimetidine for 14 days had a significantly shorter graft survival (20.2 +/- 5.1 and 11.1 +/- 2.6 days, r ... | 1991 | 1797288 |
| homeostatic equilibrium of l-carnitine family before and after i.v. administration of propionyl-l-carnitine in humans, dogs and rats. | propionyl-l-carnitine is a minor component of l-carnitine family which, when exogenously administered, proved to possess interesting cardiovascular activities. in this paper the pharmacokinetics of propionyl-l-carnitine was investigated in humans, dogs and rats after intravenous administration. in all the three species the base homeostatic equilibrium was carefully investigated in plasma and in urine during the 24 h period before the administration. propionyl-l-carnitine, acetyl-l-carnitine, l-c ... | 1991 | 1820908 |
| human placental lactogen concentration during physiological fluctuations of serum glucose in normal pregnant and gestational diabetic women. | oral glucose tolerance tests (ogtt) were carried out in 9 normal pregnant women and 11 non-obese gestational diabetics in late pregnancy. all samples were analysed for the content of glucose, insulin and placental lactogen hormone (hpl). furthermore, spontaneous changes in the serum hpl concentration during a 3 h period were studied in 6 normal women in the 2nd half of pregnancy. during ogtt only small and insignificant changes in the level of hpl were observed in both the normal subjects and th ... | 1975 | 1242271 |
| calcium dependence of effects of endothelin on rat mesenteric microvessels. | we investigated the calcium dependence of the effects of endothelin (et) on resistance vessels (less than 300 microns lumen diameter) from the mesenteric vascular bed of the rat, mounted on a wire myograph. et-1 induced a potent sustained contraction with an ed50 of 12 nmol/l. the response to et-3 and big et at the maximum concentrations used (100 nmol/l) was less than 40% of that to et-1, with an estimated ed50 of 45 nmol/l. relaxation of the et-1-induced contraction was slow, and resulted in a ... | 1991 | 1913326 |
| in vivo use of a dual acid etch biopsy for the evaluation of lead profiles in human surface enamel. | two successive acid etch biopsies were performed on the permanent maxillary right central incisors of two age-groups of children resident in an urban area in belgium. lead was determined in the biopsy solutions and the concentrations were related to etch depths which were calculated from the calcium and phosphorus concentrations in the biopsy solutions. the mean lead concentration of the first enamel biopsy layer was five times higher than in the second biopsy layer. a strong relation was observ ... | 1991 | 1913763 |
| corticotropin-releasing hormone is a rapid and potent convulsant in the infant rat. | corticotropin-releasing hormone (crh) administered into the cerebral ventricles of rats during the first postnatal week caused a specific and stereotyped behavior sequence: rhythmic chewing and licking (jaw myoclonus) were followed by 'limbic'-type seizures. the onset of the seizures was much more rapid (2-45 min vs 3-7 h) than in adult rats, and the convulsant doses were much lower (50 x 10(-12) mol per gram brain weight vs 750 x 10(-12) mol per gram brain weight in adults). crh potency in indu ... | 1991 | 1914160 |
| cancer detection with human peripheral lymphocytes: a word of caution. | | 1975 | 1196164 |
| cardiomyopathy and woolly haircoat syndrome of hereford cattle. | | 1991 | 2043089 |
| purification and characterization of human kidney cytosolic cysteine conjugate beta-lyase activity. | the central role of cysteine conjugate beta-lyase (beta-lyase) in the bioactivation of nephrotoxic halogenated hydrocarbons and the possibility of human exposure to these chemicals has focused interest on the beta-lyase from human kidney. human kidney tissue was collected as surgical waste material, and subcellular fractions were isolated by differential centrifugation. human beta-lyase activity, determined with s-(2-benzothiazolyl)-l-cysteine (btc) as the substrate, was present in the cytosolic ... | 1990 | 2139845 |
| lack of pqq dependent glucose dehydrogenase apoenzyme in some acinetobacter spp. | seventy four asaccharolytic strains of acinotobacter were tested for the presence of pqq dependent glucose dehydrogenase apoenzyme. this apoenzyme could not be detected in any of five strains of acinetobacter haemolyticus; it was also lacking in one strain of each a. junii and a. lwoffi. | 1990 | 2176072 |
| hypoglycemia risk during exercise after intramuscular injection of insulin in thigh in iddm. | the influence of bicycle exercise (60% of w170 [working capacity at a pulse rate of 170 beats/min]; 40 min) on the absorption of 125i-labeled fast-acting insulin (10 u; actrapid human insulin) after intramuscular compared with subcutaneous injection in the thigh was studied on 2 consecutive days in 10 insulin-dependent diabetes mellitus (iddm) patients. insulin absorption was measured as disappearance of radioactivity (1st-order elimination rate constants) by continuous external monitoring and a ... | 1990 | 2190773 |