| confirmation of conductance increase by adrenalin in the guinea-pig taenia coli (alpha-action). | | 1977 | 21402 |
| an adenosine 3':5'-monophosphate-adenosine binding protein from mouse liver. factors affecting the activation of the binding protein by adenosine 5'-triphosphate. | | 1978 | 23726 |
| slow encephalopathies, inflammatory responses and arachis oil. | there is much interest in the possibility that diets rich in some plant oils may be of prophylactic and curative value in certain clinical conditions. this is generally attributed to the immunosuppressive effects of polyunsaturated fatty acids. arachis (peanut) oil injections can increase the incubation period of experimental scrapie in mice. in the late 1950s several workers showed that arachis and some other oils contained very potent anti-inflammatory components quite apart from the polyunsat ... | 1975 | 47424 |
| alpha2-macroglobulin on human vascular endothelium. | alpha2-macroglobulin (alpha2m) has been identified on the luminal surface of endothelial cells in sections of normal human arteries, veins, and lymphatics by the indirect immunofluorescent technique. the specificity of the immunofluorescent reaction was confirmed by immunoabsorption studies. prior absorption of the anti-alpha2m antiserum by purified alpha2m at equivalence completely inhibited endothelial surface as well as hepatic parenchymal cell staining. endothelial cells in blood vessels wer ... | 1976 | 58955 |
| letter: macrophages v. cancer. | | 1976 | 59265 |
| response to hormones of cells cultured from human giant cell tumors of bone. | giant cell tumors of bone obtained from 7 patients were dispersed with clostridial collagenase and trypsin and adherent cells were maintained in culture. early cultures contained both mononucleated and multinucleated cells presumably derived from the stromal and giant cells of the original tumor. the original multinucleated cells did not survive for greater than 7-10 days whereas the mononucleated cells persisted and could be passaged by trypsinization. in 5 of 7 early cultures exposed to parath ... | 1978 | 87400 |
| [antigenic structure of the l forms of br. abortus 544]. | the gel precipitation test after ouchterlony and mancini was employed to study comparatively the antigenic structures of s and l forms of brucella abortus 544. reduction of the antigenic fractions was established with the l-transformed brucella organisms as compared to the initial cultures. reduced were mainly fractions of the antigenic structures that took part in cell wall building. cytoplasmic antigens remained unchanged. those that participated in the building of cell walls precipitated in c ... | 1979 | 92852 |
| relatedness among coagulase-negative staphylococci: deoxyribonucleic acid reassociation and comparative immunological studies. | dna-dna-homology values were determined under restrictive to relaxed reassociation conditions with type strains and some additional strains of coagulase-negative staphylococci belonging to ten different species. the immunological relationship of the catalases present in the type strains of these species was also determined by applying double immunodiffusion and microcomplement fixation. the results of these studies support the previous proposal to subdivide the coagulase-negative staphylococci i ... | 1979 | 92972 |
| [two-layer rocket-electrophoresis on cellulose-acetate membranes]. | two-floor rocket-electrophoresis on gelatinated acetate cellulose membrane "cellogel" has been developed. the method is based on electroimmunodiffusion detection of the antigen on the acetate-cellulose membranes, containing monospecific antiserum of the test-system. the procedure is followed by the detection of precipitation bands ("rockets") by staining, if the reaction is conducted in the visible zone or by the further treatment of the acetate-cellulose strips, containing invisible precipitate ... | 1979 | 93004 |
| immunological properties of peptic fragments of bovine serum albumin. | the effect of peptic degradation on the immunological and antigenic properties of bovine serum albumin (bsa) was investigated. molecular fragments obtained after various times of digestion (3-360 min) were studied. enzymatic digestion resulted in a rapid loss of serologically defined antigenic determinants. the immunogenicity of the fragments as measured by the level of reaginic and total anti-bsa antibody response in bdf1 mice was also diminished. pre-treatment of mice with fragments exhibiting ... | 1979 | 93079 |
| occurrence and subcellular localization of glucose 6-phosphatase in bovine thyroid. | in bovine thyroid tissue the glucose 6-phosphatase activity is not entirely due to the presence of an unspecific acid phenylphosphatase nor to beta-glycerophosphatase. this glucose 6-phosphatase is very probably localized within endoplasmic reticulum membranes. it is not a good marker for distribution patterns obtained after differential pelleting. however it can be used as a marker for endoplasmic reticulum membranes after centrifugation in a zonal rotor. | 1979 | 93889 |
| exposed sulphydryl groups of types of haemoglobin during human ontogenesis. | | 1978 | 94245 |
| correlation between pharmacokinetics and intropic and electrophysiologic responses to digitoxin in the intact dog. | we attempted to correlate inotropic and eletrophysilogic digitoxin effects with serum digitoxin concentrations during 8 hr after a single dose. eight pentobarbital-anesthetized labrador dogs were given 2.0 mg digitoxin intravenously. left heart catheterization and his bundle registration were performed. acing and programed electrical stimulation were used to determine heart rate-independant changes in dp/dt, intra-atrial, atrioventricular (av), his-purkinje, and intraventricular conduction veloc ... | 1979 | 94409 |
| a detergent-citric acid technique for isolating nuclear and cytoplasmic fractions containing undegraded rna from cells of xenopus laevis. | | 1979 | 94513 |
| application of uracil-bonded silica gel to the separation of adenine and its derivatives, and to poly(a)-containing rna. | | 1979 | 94919 |
| [influence of parotin on the repressing action of cyanylethyl group to histamine stress (author's transl)]. | | 1978 | 95057 |
| isolation of the l-phase variant from toxigenic corynebacterium diphtheriae c7(beta). | the l-phase variant was isolated from lysogenic strain c7(beta) of corynebacterium diphtheriae by the penicillin disk tecnnique on an agar medium containing 3.7% brain heart infusion, 3% nacl, 1.25% grycine, 1% noble agar, and 10% horse serum. the l-phase formed a "fried egg" colony; it was resistant to antibiotics that inhibit biosynthesis of bacterial cell walls but was susceptible to antibiotics that act on the protein-synthesizing system. after several transfers of the l-phase initially isol ... | 1978 | 97222 |
| [experience with the use of the coulter counter model "s" in the field of animal experimentation]. | | 1978 | 98930 |
| pharmacological studies of nitroharmidine nitrate on central nervous system. | | 1978 | 99533 |
| ineffectiveness of prophylactic intrathecal methylprednisolone in myelography with aqueous media. | the effectiveness of prophylactic intrathecal methylprednisone (mp) in preventing arachnoiditis from iocarmate myelography was studied. monkeys were injected intrathecally with locarmate, mp, or a combination of the two. twelve weeks later the severity of arachnoiditis was determined in each animal. there was no significant prophylactic effect of intrathecal mp on arachnoiditis from locarmate. some arachnoiditis was found in control animals treated with mp alone. | 1978 | 99764 |
| congeners of dom: effect of distribution of the evaluation of pharmacologic data. | | 1978 | 101880 |
| a new sequence-specific endonuclease from anabaena cylindrica. | | 1978 | 103749 |
| [endogenous morphine-like peptide]. | | 1978 | 107051 |
| [effect of amikacin on experimental tuberculosis of mice (author's transl)]. | | 1979 | 107361 |
| serotype-dependent inhibition of glucan synthesis and cell adherence of streptococcus mutans by antibody against glucosyltransferase of serotype e s. mutans. | a crude glucosyltransferase (gtase) preparation was obtained from the culture supernatant of streptococcus mutans strain mt703 (serotype e) by 50% ammonium sulphate precipitation. antiserum specific against the gtase was prepared by immunizing rabbits intramuscularly with the gtase in freund incomplete adjuvant, followed by gtase without adjuvant intravenously. gamma globulin fractions of the antiserum and normal serum were partially purified by 1/3 saturated ammonium sulphate precipitation. the ... | 1979 | 111004 |
| third generation of systemic adjuvants of immunity: experimental basis for adjuvant combinations. | | 1978 | 111325 |
| pharmacological modification of experimental tardive dyskinesia. | cebus apella monkeys subjected to chronic haloperidol administration develop neurologic disturbances very similar to neuroleptic-induced acute dystonia human beings. after varying lengths of time, certain monkeys develop a prolonged dyskinetic syndrome resembling tardive dyskinesia (td), as seen clinically. two monkeys with signs of td were given single intramuscular injections of various compounds with known effects on the catecholaminergic, cholinergic, serotoninergic and gaba-ergic neurotrans ... | 1979 | 115227 |
| [enzymes catalyzing the reduction of aldopentoses and the oxidation of pentitols in mycobacteria]. | m. phlei, grown on synthetic sauton medium (with 6% glycerol as carbon source), had nadph- and nadh-aldopentose reductase, as well as nad-pentitol dehydrogenase activities; some of their properties are studied. these activities are not present in bcg grown on the same medium. all experiments of aldopentose-reductase induction in bcg on a d(+)xylose medium were negative. | 1979 | 120778 |
| [radioimmunoassay without extraction of l-triiodothyronine in human serum]. | a radio-immunoassay of l-triiodothyronin is described. blinding of t3 to tbg is prevented by salicylate (1%--w/v). the rabbit antiserum obtained by injection of a complexe t3-bovine serum albumin is diluted between 1/10000 and 1/20000. bound and free hormone are separated by polyethylene glycol (17%--w/v). serum t3-concentration in normal subjects averaged 132 +/- 29 ng/dl (sd). in hyperthyroid and hypothyroid patients, these values were respectively 351 +/- 118 ng/dl and 68 +/- 21 ng/dl. | 1975 | 126769 |
| [study of the incorporation of radioactive zinc in saccharomyces cerevisiae aldolase]. | saccharomyces cerevisiae aldolase concentrates from the culture medium containing znso4 a large amount of zn which becomes a component part of the enzyme molecular structure. this was made evident by adding to the culture medium 65znso4 and measuring the radioactivity of the aldolase extracted by a phillips single channel analyzer. | 1976 | 133445 |
| [use of intraspecific differentiation of sh. sonnei for the purpose of studying patterns in the epidemic process of dysentery]. | the use of complex typing of sh. sonnei by the colicinogenic and enzymatic properties in epidemiological analysis of dysentery caused by sh. sonnei, apart from establishment of epidemiological connections in the foci, offered a possibility of introducing significant corrections in the determination of the sizes and duration of existence of the epidemic foci, as well as in detection of the sources and factors of transmission of the infection established in epidemiological examination. typing of s ... | 1978 | 154276 |
| the effect of beef spleen cathepsin d on pig heart lactate dehydrogenase. | | 1975 | 166614 |
| ultrastructural demonstration of phosphatases by perfusion fixation followed by perfusion incubation of rat liver. | an alternative to previous methods (tissue chopper, frozen sections) for the ultrastructural demonstration of phosphatases is described. the present approach is based on a short vascular perfusion of rat liver with glutaraldehyde through the inferior caval vein, followed by vascular perfusion incubation with a medium containing the enzyme substrates. the effect of glutaraldehyde on three different types of phosphatases was investigated, namely a lysosomal enzyme (acid phosphatase) a tightly boun ... | 1975 | 166967 |
| pigmentation and dysfunction of gunn rat thyroid: correlation between morphological and biochemical data. | the thyroid gland of homozygous gunn rats is moderately enlarged and displays a brownish-black discoloration. light microscopic examination discloses that the follicular cells are filled with brown granules, which are shown, under the electron microscope, to be modified colloid droplets. most of them possess a strong acid phosphatase and a mild peroxidase activity and contain a melanin-like pigment, according to histochemical analysis. in comparison with normal wistar rats, gunn rats possess sig ... | 1976 | 176774 |
| in vitro rna transcription by the new jersey serotype of vesicular stomatitis virus. i. characterization of the mrna species. | the in vitro rna synthesis by the virion-associated rna polymerase of vesicular stomatitis virus (vsv), new jersey serotype, was compared with that of the serologically distinct indiana serotype of vsv. the new jersey serotype of vsv synthesized five distinct mrna species in vitro, three of which were smaller than the corresponding species synthesized by the indiana serotype of vsv. these included the mrna's coding for the g, m, and ns proteins. by hybridization experiments, virtually no sequenc ... | 1978 | 206724 |
| [studies on the typing of a foot-and-mouth disease strain (type a)]. | | 1978 | 208502 |
| isolation of the proviral coding strand of avian myeloblastosis virus from leukemic chicken myeloblast by affinity chromatography. | | 1978 | 210689 |
| a rapid and sensitive fluorescence assay for angiotensin-converting enzyme. | | 1978 | 210690 |
| the influence of age on the wound healing of experimental myocardial infarction in rats. | in 6-weeks-old, 5-months-old and 21-months-old rats myocardial infarction was induced by coronary artery ligature. after performing the ligature the animals were administered 3h-thymidine, 3h-proline or 35s-sulphate at different times. the following parameters were determined: number of dna- and tropocollagen-synthesizing connective tissue cells at the infarction border and at the infarction site; mean silver grain density above the nuclei or cells; duration of a cycle; number of mitoses, and in ... | 1975 | 134906 |
| characterization of nadh-cytochrome c reductase, a component of benzoate 1,2-dioxygenase system from pseudomonas arvilla c-1. | nadh-cytochrome c reductase, a component of benzoate 1,2-dioxygenase system, was purified to homogeneity, as judged by sodium dodecyl sulfate disc gel electrophoresis and ultracentrifugation, from benzoate-induced cells of pseudomonas arvilla. the molecular weight of the enzyme was determined to be 38,300 by sedimentation equilibrium analysis, 37,000 by sephadex g-100 gel filtration, and 37,500 by sodium dodecyl sulfate disc gel electrophoresis, respectively, indicating that the enzyme consisted ... | 1978 | 214433 |
| [nuclei of the mammillary bodies and their fiber connections in cattle (bos taurus var. domesticus). i. nuclei of the mammillary bodies in cattle]. | 1. in the present neuroanatomic study the topography and cytoarchitectonic of the nuclei of the mamillary body of cattle are described and quantitative-morphologically investigated in 14 series of brains (6 frontal, 4 sagittal and 4 horizontal series, stained by the methods after nissl, klüver and barrera and after heidenhain). 2. the medial mamillary nucleus of cattle is with an average volume of 47.7 mm3 the largest nucleus of the mamillary body. it contains about 715,000 cells with a cell den ... | 1978 | 107664 |
| primary meningococcal conjunctivitis in an infant. | a case of primary meningococcal conjunctivitis in an infant was presented and its treatment is discussed. because of the potential for systemic spread of the infection in the patient, local and systemic therapy was instituted. the current literature on meningococcal conjunctivitis with its potential complications is briefly reviewed. | 1979 | 106998 |
| application of a new surface labeling reagent, edta derivative, on erythrocytes and platelets. | the modes of binding of a new class of impermeant metal-chelating probe, the complex of 111in3+ to 1-(p-benzenediazonium) ethylenediamine tetraacetic acid (azo-phenyl-edta), to human and rabbit erythrocyte membranes and the effect of binding on the function of rabbit platelets have been studied. the metal chelate, azo-phenyl-edta.[111in3+] bound covalently to membrane proteins following reaction with intact erythrocytes. the amount and the pattern of labeling was assessed by sodium dodecyl sulfa ... | 1978 | 100139 |
| roles of peptides in central transmission: substance p, bradykinin and b-endorphin. | | 1979 | 95614 |
| regulation of the surface physical properties of the very low density lipoprotein. | | 1979 | 218937 |
| [stress and immunity in cattle]. | stress of variable length may be caused in calf by parenteral administration of supercortisol and application of acute thermal load. hormonal or thermal stress may have negative impact on postvaccinal immunity to paratyphoid (salmonella dublin 81), as may be interpreted with reference to the o-agglutination titre of blood serum. the extent to which immunity is suppressed by stress was found to depend on the moment of stress application, duration of stress, and age of the calf. | 1979 | 222226 |
| infection studies on a reticuloendotheliosis virus contaminant of a commercial marek's disease vaccine. | reticuloendotheliosis virus (rev) was isolated in cell cultures from commercial marek's disease (herpesvirus of turkeys) vaccine and re-isolated from the organs of vaccinated chickens. runting and feathering abnormalities were produced when 1-day-old specific pathogen free chickens were inoculated with rev. histopathological lesions in infected chickens were hypoplasia of the thymus, bursa and spleen, and inflammation of the proventriculus, kidneys and liver. serological responses to rev were de ... | 1979 | 223539 |
| [demonstration of the oncogenic activity of cell cultures using antithymocytic serum]. | the authors examined 10 cell strains of different origin as to their effect on mice by means of antithymocytic (atc) serum. in dependence on the strain used the tumors developed in different number and with different growth tendency. in control animals not treated by the atc serum, small ganglions developed in some cases which, however, disappeared in 2--3 days. both strains of diploide cells wi 38 and lep and primary cultures of chicken fibroblast from embryos of spf chickens did not develop an ... | 1978 | 311797 |
| atp induces nucleotide permeability in rat mast cells. | | 1979 | 450099 |
| pyrazolidine derivatives: a comparative study of their effects on platelet aggregation. | quantitative studies were carried out of the in vitro and ex vivo effects of phenylbutazone and 3-oxoalkyl substituted diphenyldioxopyrazolidines (kebuzone, tribuzone, benzopyrazone) on platelet aggregation. the specified pyrazolidine derivatives exhibited in vitro inhibitory effects on secondary platelet aggregation (induced by adrenaline and collagen), commensurable with the effects of sulfinpyrazone. the ex vivo efficacy was markedly influenced by the height of the drug level in blood and by ... | 1979 | 94876 |
| equilibrium saturation chromatographic method for studying the binding of ligands to human serum albumin by high-performance liquid chromatography. influence of fatty acids and sodium dodecyl sulphate on warfarin-human serum albumin binding. | a size exclusion chromatographic method for studying ligand-macromolecule binding parameters is described. this equilibrium saturation method allows the determination of the concentrations of constituents in equilibrium and is specially useful for characterizing ligand--protein binding under conditions that can be compared with physiological conditions. the method has been used for measuring warfarin--human serum albumin (hsa) binding and for studying the influence of free fatty acids (ffa) and ... | 1979 | 541445 |
| isolation of bovine brain microtubule protein without glycerol: polymerization kinetics change during purification cycles. | | 1979 | 543557 |
| dependence of macromolecular composition and morphology of streptomyces hygroscopicus on specific growth rate. | the dependence of macromolecular composition and morphology of streptomyces hygroscopicus on specific growth rate micron was investigated. the percentage of dna on dry weight (%dna) is constant, % protein is also nearly independent of micron whereas %rna rises considerably with increasing micron, regarding mycelia grown in glucose-limited and ammonium-limited continuous cultures as well as in discontinuous cultures with various carbon sources. it is probable that the overall synthesis of dna, rn ... | 1979 | 94484 |
| macrophage-lymphocyte clusters in the immune response to soluble protein antigen in vitro. viii. cinephotomicrographic studies. | t cells from immune guinea-pigs produce clusters in vitro with macrophages pulsed with soluble protein antigens. the formation of clusters is antigen-specific. time-lapse cinephotomicrography was used to study the sequence of events leading to cluster formation and the kinetics of already formed clusters. the central lymphocyte attaches through a broad base to the macrophage during the first few hours of incubation. after several hours, during which the central lymphocyte is situated alone on th ... | 1979 | 94460 |
| antigenic components of taenia saginata and their relevance to the diagnosis of bovine cysticercosis by immunoelectrophoresis. | 22 antigenic components of taenia saginata proglottides were detected by immunoelectrophoretic (iep) study. cross-absorption of the hyperimmune serum, raised in rabbits against t. saginata, with heterologous and host antigens showed that two thirds of these components were not specific for the cestode. among the calves experimentally infected with t. saginata eggs, 3 precipitation patterns in iep were identified depending on the evolutionary stage of the infection. the hydrosoluble extract of t. ... | 1979 | 94334 |
| decreased axonal flux of retrogradely transported glycoproteins in early experimental diabetes. | | 1979 | 91664 |
| automatic cell identification and enrichment in lung cancer. iii. light scatter and two fluorescence parameters. | two fluorescence parameters and size are used in a flow through system to enrich sputum specimens for cancer cells. human cells in sputum which are stained with acridine orange show a characteristic distribution of red and green fluorescence from which cancer cells can be localized. the peak enrichment is obtained by selectively sorting cells with the largest values of red and green fluorescence. cancer cells located in other distribution regions having smaller fluorescence intensities show prog ... | 1979 | 86577 |
| the selective suppression of an antibody response to a given antigen. | | 1976 | 1084334 |
| cultivation of viral agents from crohn's disease. a new sensitive system. | the isolation and animal transmission of a viral agent from crohn's disease patients stimulated studies aimed at the development of improved tissue culture techniques. the need for an effective tissue-culture system led to a comparison of african green monkey tissue-culture cells (a.g.m.k.), human diploid lung cells (wi-38), and a new tissue-culture line--continuous rabbit ileum (c.r.i.). homogenates prepared from ileal specimens from four crohn's disease patients and from four control patients ... | 1976 | 59237 |
| [march hemoglobinuria. one case with erythrocyte glutathione peroxidase deficiency]. | a new case of march haemoglobinuria seen in a 21-year-old man is reported. a deficiency in two erythrocytic enzymes (glutathione reductase and glutathione peroxydase) was observed. treatment with riboflavin corrected the glutathione reductase deficiency. the march haemoglobinuria and glutathione peroxydase deficiency persisted. several months after resolution of the march haemoglobinuria, a new estimation of glutathione peroxydase showed that the deficiency had disappeared. the possible role of ... | 1975 | 1134932 |
| the use of ionophores of rapid loading of human red cells with radioactive cations for cation-pump studies. | techniques are described for the rapid loading of intact human red cells with radioactive isotopes of alkali cations or ca2+ by using ionophorous compounds (nigericin, gramicidin d and a 23187). loading was rapid and efficient if the membrane potential of the cells was rendered more negative inside. after cation loading the ionophores could be bound to albumin and removed by repeated washings. the atp and 2,3-dpg contents of the cells were practically unaltered by this treatment. passive membran ... | 1976 | 58995 |
| oxidation and metabolism of linoleic acid in the sheep. | | 1975 | 1187648 |
| stoichiometry of h+ translocation coupled to electron flow from succinate to cytochrome c in mitochondria. | | 1979 | 40984 |
| proceedings: the uptake of adenine by isolated human platelet membranes. | | 1975 | 1198511 |
| [repeated obtaining of dog peritoneal macrophages and their cultivation in vitro]. | | 1975 | 1214674 |
| effect of natural variations in paco2 on plasma [hco3-] in dogs: a redefinition of normal. | graded degrees of both chronic hyper- and hypocapnia are known to induce renal responses that significantly alter plasma bicarbonate concentration. these findings have raised the possibility that even normal variations in paco2 play an important role in determining the exact level of bicarbonate in plasma. to test this hypothesis, we examined the relationship between resting levels of paco2 and the plasma bicarbonate concentration in two groups of normal dogs, one ingesting a normal salt diet an ... | 1979 | 35008 |
| [test of degranulation of basophils and mast cells for detection of allergic diseases (literature survey)]. | | 1976 | 1274295 |
| isolation and characterization of the acidic phosphoproteins of 60-s ribosomes from artemia salina and rat liver. | eucaryotic l7/l12-type proteins are present in ethanol/salt extracts (p1 protein) of ribosomes from artemia salina and rat liver. these proteins are partially phosphorylated and occur in two forms of closely related structure: a major form el12 having methionine at the n-terminal position and a minor form of el12 (el12') which seems slightly elongated and contains a blocked n terminus. purification of the four different forms of this protein, el12, el12-p, el12' and el12'-p, was performed by ion ... | 1978 | 32034 |
| biochemical differentiation of aggregating cell cultures of different fetal rat brain regions. | rotation-mediated aggregating cell cultures of mechanically dissociated fetal rat brains divided into three (telencephalon, mesencephalon-diencephalon and rhombencephalon), or two (telencephalon and mesencephalon-diencephalon plus rhombencephalon) parts were examined for their biochemical differentiation by measuring the specific activities of choline acetyltransferase, acetylcholinesterase, glutamic acid decarboxylase, tyrosine 3-monooxygenase, aromatic l-amino acid decarboxylase, catechol meth ... | 1977 | 20195 |
| inhibition of protein synthesis by glucose 6-phosphate and fructose 1,6-diphosphate in lysed rabbit reticulocytes and the reversal of inhibition by nad+. | | 1978 | 212031 |
| preparative isoelectric focusing of human serum very low-density and low-density lipoproteins. | | 1978 | 211872 |
| effect of galactose on free radical reactions of polymorphonuclear leukocytes. | | 1978 | 209746 |
| type-c rna viruses and leukemogenesis: relation of type-c virus infectivity and leukemogenesis induced by nitrosourea compounds in mice. | correlation between infectivity of type-c rna virus) murine leukemia virus, mlv) and development of leukemia was tested in female icr/jcl mice treated with either 1-ethyl-1-nitrosourea (enu) or 1-butyl-1-nitrosourea (bnu). continuous administration of either chemicals resulted in the occurrence of thymic lymphoma in every mouse with a short latent period. the time of appearance and distribution pattern of mlv infectivity in various tissues were examined by the xc plaque assay technique at fixed ... | 1976 | 187521 |
| an in vitro study of the effect of photodynamic therapy on human meningiomas. | despite being benign cns tumours, meningiomas are not always curable and the likelihood of recurrence depends upon the completeness of initial removal. adjuvant therapy for incompletely resected meningiomas is generally unsatisfactory and such lesions continue to pose difficult management problems. photodynamic therapy (pdt) has been employed in the management of recurrent cerebral gliomas but its activity against meningiomas has not been specifically studied. an in vitro study of the effects of ... | 1992 | 1388826 |
| recovery of dna from agarose gels stained with methylene blue. | | 1992 | 1389151 |
| [immune response to htlv-i infection]. | | 1992 | 1413533 |
| [effect of biogenic amines on glycogen degradation in isolated rat hepatocytes]. | serotonin and tyramine affected dissimilarly the enzyme activity involved in degradation of glycogen in isolated rat hepatocytes. serotonin inhibited hydrolytic enzymes acid alpha-glucosidase, alpha-amylase and amylo-1,6-glucosidase but activated phosphorylase inducing a decrease of glycogen content in cells. tyramine inhibited acid alpha-glucosidase and amylo-1,6-glucosidase, did not affect the alpha-amylase and phosphorylase activities and increased content of glycogen in cells. tyramine may b ... | 1992 | 1413626 |
| studies on the pituitary "fettstoffwechselhormon". vi. preparation of two highly purified lipolytic active peptides from hog pituitary glands. | the preparation of two highly purified lipolytically active hog pituitary peptides, called p-lf ii c and p-lf ii d is described. the two peptides are free of other pituitary hormone activities. in isolated rat and porcine adipose tissue, both fractions are lipolytically much more active than every other lipolytic active pituitary peptide described to date. by fraction p-lf ii d, the first pituitary peptide was isolated which has lipolytic activity in isolated rat adipose tissue than corticotropi ... | 1976 | 181302 |
| mycobacterium fortuitum infections of the hand. report of five cases. | five cases are reported of infection due to mycobacterium fortuitum involving the hand following contaminated injection or traumatic wounds. synovectomy, debridement, or amputation together with prolonged chemotherapy using kanamycin or amikacin were required. doxycycline and sulphamethoxasole also seemed to be the effective antibiotics for this organism. a high index of suspicion is important in order to obtain the correct diagnosis. | 1992 | 1484253 |
| mouse or man? which are pertussis vaccines to protect? | type 1 strains of bordetella pertussis can infect mouse brain and have been recovered as type 1 organisms after death. when introduced into the naso-pharynx of the marmoset, they immediately acquired agglutinogen 2 or 3, and the resulting type 1,2 or 1,3 infection persisted for many weeks. as in the child, agglutinogens 2 and/or 3 appear to be essential for infection of the marmoset, whereas they are quite unnecessary in mouse brain. a vaccine (extract or whole cell) containing agglutinogen 1 ma ... | 1976 | 177701 |
| acupuncture analgesia. | acupuncture-produced surgical analgesia has been demonstrated in a wide variety of species under experimental and clinical circumstances. the main advantage is that no depressant drugs need to be used. the disadvantages of its use are unfamiliarity, the need for special equipment, inconsistent effects, and lack of restraint. | 1992 | 1533972 |
| diagnosis of sickle cell disease in chronically transfused patients. | standard electrophoretic methods for the diagnosis of hemoglobinopathies are confounded in individuals chronically transfused. we present the accurate diagnosis of sickle cell disease in two such transfused patients by the application of polymerase chain reaction technology to analyze patient's hemoglobin beta-chain genes directly. | 1992 | 1550267 |
| isolation of a human plasmin-derived, functionally active, light (b) chain capable of forming with streptokinase an equimolar light (b) chain-streptokinase complex with plasminogen activator activity. | a functionally active human plasmin light (b) chain derivative, stabilized by the streptomyces plasmin inhibitor leupeptin, was isolated from a partially reduced and alkylated enzyme preparation by an affinity chromatography method with a l-lysine-substituted sepharose column. this light (b) chain derivative was found to be relatively homogeneous by electrophoretic analysis in both an acrylamide gel/dodecyl sulfate system and on cellulose acetate. it possessed approximately 3% of the proteolytic ... | 1976 | 134997 |
| inhibition of nitric oxide synthase blocks n-methyl-d-aspartate-, quisqualate-, kainate-, harmaline-, and pentylenetetrazole-dependent increases in cerebellar cyclic gmp in vivo. | the synthesis of nitric oxide by brain slices has been demonstrated in several laboratories. in addition, in vitro studies have demonstrated stimulation of nitric oxide synthesis by excitatory amino acid receptor agonists. these data have led to the hypothesis that this readily diffusible "intercellular messenger molecule" acts to generate a cascade effect by activating guanylate cyclase in several cell types and thereby augment levels of the second messenger cyclic gmp (cgmp). therefore, we eva ... | 1990 | 1693947 |
| peptide regulation of adrenal medullary function. | the discovery of peptides in the splanchnic nerve and adrenal gland, and their co-existence with conventional neurotransmitters raises questions about their possible functional roles in catecholamine (ca) secretion and gene transcription in the adrenal gland. short-term, stress-induced ca secretion is regulated biphasically by substance p (sp) which inhibits acetylcholine (ach) action at sp greater than 10(-6) m and facilitates ca secretion in response to metabolic and physical stressors, ach or ... | 1990 | 1694230 |
| clinical, neuropathologic and pharmacologic aspects of huntington's disease: correlates with a new animal model. | | 1977 | 214805 |
| human carotid artery diameter and flow by a noninvasive technique. | this paper presents a technique tested in vitro and in dogs and used in humans to measure carotid artery blood flow continuously by placing a transducer on the skin over the artery. the tranducer consists of a pulse echo crystal used to locate the carotid artery and measure its diameter and wall velocity. it also has a pair of doppler shift crystals used to measure the velocity of the artery wall and blood flow in the vessel. it was found that the pulsatile artery wall distension was between 8 a ... | 1975 | 124007 |
| allergy to rabbits. iii. further identification and characterisation of rabbit allergens. | crossed immunoelectrophoresis and crossed radio-immunoelectrophoresis techniques have been employed for analysis of extracts of various rabbit source materials to identify rabbit allergens in addition to the already described antigen r1 (agr1). urine and dander extracts were found to contain only low levels of agr1 and its presence in urine was as a contaminant due to mode of collection--it was not present in urine collected directly from the bladder. other allergens were only recognised by high ... | 1991 | 1796773 |
| inhibitory effect of cimetidine on graft survival of allotransplanted rats submitted to an active-passive enhancement protocol. | the objective of the present study was to determine whether cimetidine, a type-2 histamine receptor antagonist, inhibits the immunological enhancement of allografted rats achieved by treatment with donor antigen plus anti-donor antibody. groups of rats submitted to this active-passive enhancement protocol and treated ip with 30 (apec 30; group i; n = 4) or 60 (apec 60; group ii; n = 8) mg/day cimetidine for 14 days had a significantly shorter graft survival (20.2 +/- 5.1 and 11.1 +/- 2.6 days, r ... | 1991 | 1797288 |
| inhibitory effect of cimetidine on graft survival of allotransplanted rats submitted to an active-passive enhancement protocol. | the objective of the present study was to determine whether cimetidine, a type-2 histamine receptor antagonist, inhibits the immunological enhancement of allografted rats achieved by treatment with donor antigen plus anti-donor antibody. groups of rats submitted to this active-passive enhancement protocol and treated ip with 30 (apec 30; group i; n = 4) or 60 (apec 60; group ii; n = 8) mg/day cimetidine for 14 days had a significantly shorter graft survival (20.2 +/- 5.1 and 11.1 +/- 2.6 days, r ... | 1991 | 1797288 |
| homeostatic equilibrium of l-carnitine family before and after i.v. administration of propionyl-l-carnitine in humans, dogs and rats. | propionyl-l-carnitine is a minor component of l-carnitine family which, when exogenously administered, proved to possess interesting cardiovascular activities. in this paper the pharmacokinetics of propionyl-l-carnitine was investigated in humans, dogs and rats after intravenous administration. in all the three species the base homeostatic equilibrium was carefully investigated in plasma and in urine during the 24 h period before the administration. propionyl-l-carnitine, acetyl-l-carnitine, l-c ... | 1991 | 1820908 |
| characterization of activation and deactivation pathways of 4-(n-nitrosomethylamino)-1-(3-pyridyl)-1-butanone (nnk) in rat hepatocytes. | we have characterized the metabolism of 4-(n-nitrosomethylamino)-1-(3-pyridyl)-1-butanone (nnk) in cultured rat hepatocytes and have established the relationship between various metabolic pathways and single-strand breaks (ssb) in dna. metabolism of [5-3h]-nnk by carbonyl reduction, alpha-carbon hydroxylation and pyridine n-oxidation was linear from 0.5 to 6 h with 0.25-2 x 10(6) hepatocytes. using an alkaline elution assay, we observed that nnk induces ssb in dna in a dose- and time-dependent m ... | 1991 | 1855907 |
| calcium dependence of effects of endothelin on rat mesenteric microvessels. | we investigated the calcium dependence of the effects of endothelin (et) on resistance vessels (less than 300 microns lumen diameter) from the mesenteric vascular bed of the rat, mounted on a wire myograph. et-1 induced a potent sustained contraction with an ed50 of 12 nmol/l. the response to et-3 and big et at the maximum concentrations used (100 nmol/l) was less than 40% of that to et-1, with an estimated ed50 of 45 nmol/l. relaxation of the et-1-induced contraction was slow, and resulted in a ... | 1991 | 1913326 |
| in vivo use of a dual acid etch biopsy for the evaluation of lead profiles in human surface enamel. | two successive acid etch biopsies were performed on the permanent maxillary right central incisors of two age-groups of children resident in an urban area in belgium. lead was determined in the biopsy solutions and the concentrations were related to etch depths which were calculated from the calcium and phosphorus concentrations in the biopsy solutions. the mean lead concentration of the first enamel biopsy layer was five times higher than in the second biopsy layer. a strong relation was observ ... | 1991 | 1913763 |
| corticotropin-releasing hormone is a rapid and potent convulsant in the infant rat. | corticotropin-releasing hormone (crh) administered into the cerebral ventricles of rats during the first postnatal week caused a specific and stereotyped behavior sequence: rhythmic chewing and licking (jaw myoclonus) were followed by 'limbic'-type seizures. the onset of the seizures was much more rapid (2-45 min vs 3-7 h) than in adult rats, and the convulsant doses were much lower (50 x 10(-12) mol per gram brain weight vs 750 x 10(-12) mol per gram brain weight in adults). crh potency in indu ... | 1991 | 1914160 |
| ontogenesis of 3,5,3'-triiodothyronine receptors in neonatal rat brain: dissociation between receptor concentration and stimulation of oxygen consumption by 3,5,3'-triiodothyronine. | | 1978 | 217661 |
| cardiomyopathy and woolly haircoat syndrome of hereford cattle. | | 1991 | 2043089 |
| purification and characterization of human kidney cytosolic cysteine conjugate beta-lyase activity. | the central role of cysteine conjugate beta-lyase (beta-lyase) in the bioactivation of nephrotoxic halogenated hydrocarbons and the possibility of human exposure to these chemicals has focused interest on the beta-lyase from human kidney. human kidney tissue was collected as surgical waste material, and subcellular fractions were isolated by differential centrifugation. human beta-lyase activity, determined with s-(2-benzothiazolyl)-l-cysteine (btc) as the substrate, was present in the cytosolic ... | 1990 | 2139845 |
| lack of pqq dependent glucose dehydrogenase apoenzyme in some acinetobacter spp. | seventy four asaccharolytic strains of acinotobacter were tested for the presence of pqq dependent glucose dehydrogenase apoenzyme. this apoenzyme could not be detected in any of five strains of acinetobacter haemolyticus; it was also lacking in one strain of each a. junii and a. lwoffi. | 1990 | 2176072 |
| hypoglycemia risk during exercise after intramuscular injection of insulin in thigh in iddm. | the influence of bicycle exercise (60% of w170 [working capacity at a pulse rate of 170 beats/min]; 40 min) on the absorption of 125i-labeled fast-acting insulin (10 u; actrapid human insulin) after intramuscular compared with subcutaneous injection in the thigh was studied on 2 consecutive days in 10 insulin-dependent diabetes mellitus (iddm) patients. insulin absorption was measured as disappearance of radioactivity (1st-order elimination rate constants) by continuous external monitoring and a ... | 1990 | 2190773 |