"synthetical" aiptasia mutabilis rapp (coelenterata) (author's transl).after being cultivated in the dark for some months and after being fed with food free of carotenoids during the time aiptasia mutabilis (coelenterata) loses its symbiontic algae (rich in brown fucoxanthin) and becomes transparent white. this disarranged symbiosis may be regenerated under light cultivation by adding different species of chlorophyceae (chlorella, dunaliella) and chrysophyceae (ochromonas, cyclotella), but not of cyanophyceae (anabaena, nostoc, oscillatoria, anacystis). - this chan ...19768888
inhibition of photosynthetic electron transport in tobacco chloroplasts and thylakoids of the blue green alga oscillatoria chalybea by an antiserum to synthetic antiserum to synthetic zeaxanthin inhibits photosynthetic electron transport on the oxygen-evolving side of photosystem ii in tobacco chloroplasts and thylakoids of the filamentous blue-green alga oscillatoria chalybea. the inhibition site lies for both species between the site of electron donation of water or tetramethyl benzidine and that of diphenyl carbazide or manganese ii ions. typical photosystem i reactions are not impaired by the antiserum. the effect of the antiserum concerning the ...197944593
a phenomenon of photoenhancement of acridine orange-induced is a common experience that, with exposure to exciting radiation of the fluorescence microscope, the acridine orange-induced red fluorescence of the nucleus, produced by feulgen hydrolysis, fades with a concomitant shift to green. the present investigation reports a phenomenon of photoenhancement observed in the hydrolyzed cytoplasm where pale green fluorescence increases in intensity with exposure to exciting radiation. the phenomenon has been noticed in rhizobium, oscillatoria, tomato root ...197658929
nitrogen-fixing communities in an intertidal ecosystem.nitrogen gixation (acetylene reduction) associated with various communities in the intertidal sand and mud flat was measured in situ. areas which were colonized by algae, plants, and animals generally had significantly higher activities than areas which were visibly uncolonized. the highest activities were measured at sites colonized by a mixed bloom of oscillatoria-euglena-photosynthetic bacteria. these areas occupied only about 1% of the surface of the sediments, yet contributed nearly 50% of ...197899219
[blooming and destruction of cyanobacteria in the drainage bassin of the hydrogen sulfide spring of staraya matsesty].the growth of cyanobacteria belonging to the genera oscillatoria and anabaena (up to 2.1 x 10(7) filaments per 1 g of wet sample) was found in a water reservoir with a high content of sulfides (up to 9 mm) in staraya matsesta throughout the year. the spots of oscillatoria are located in the spring in more illuminated areas as compared to anabaena. in the spring, not only spots of actively growing cells were detected, but also accumulations of oscillatoria cells being destroyed (blue spots). wate ...1979119145
occurrence of facultative anoxygenic photosynthesis among filamentous and unicellular cyanobacteria.eleven of 21 cyanobacteria strains examined are capable of facultative anoxygenic photosynthesis, as shown by their ability to photoassimilate co2 in the presence of na2s, 3-(3,4-dichlorophenyl)-1,1-dimethylurea and 703-nm light. these include different cyanobacterial types (filamentous and unicellular) of different growth histories (aerobic, anaerobic, and marine and freshwater). oscillatoria limnetica, aphanothece halophytica (7418), and lyngbya (7104) have different optimal concentrations of ...1977402355
photooxidation reactions of diphenylcarbazide and their dcmu-sensitivity in thylakoids of the blue-green alga oscillatoria chalybea.thylakoids of oscillatoria chalybea are able to split water. the hill reaction of these thylakoids is sensitive to dcmu. diphenylcarbazide can substitute for water as the electron donor to photosystem ii with these fully functioning thylakoids. however, the diphenylcarbazide photooxidation is completely insensitive to 3-(3,4-dichlorophenyl)-n-n'-dimethyl urea (dcmu) at high diphenylcarbazide concentrations. in with tris-treated oscillatoria thylakoids the water splitting capacity is lost and dip ...1977414682
hydrogen metabolism in the facultative anoxygenic cyanobacteria (blue-green algae) oscillatoria limnetica and aphanothece halophytica.two facultative anoxygenic photoautotrophic cyanobacteria, oscillatoria limnetica and aphanothece halophytica were found capable of co2 photoassimilation using molecular hydrogen as electron donor in a photosystem i driven reaction. a. halophytica was also capable of evolving hydrogen from na-dithionite reduced methylviologen in a light independent reaction.1978414684
induction of anaerobic, photoautotrophic growth in the cyanobacterium oscillatoria limnetica.anaerobic photoautotrophic growth of the cyanobacterium oscillatoria limnetica was demonstrated under nitrogen in the presence of 3-(3,4-dichlorophenyl)-1,1-dimethylurea (5micron), a constant concentration of na2s (2.5 mm), and constant ph (7.3). the photoanaerobic growth rate (2 days doubling time) was similar to that obtained under oxygenic photoautotrophic growth conditions. the potential of oxygenic photosynthesis is constitutive in the cells; that of anoxygenic photosynthesis is rapidly (2 ...1978415043
respiratory allergy to algae: clinical aspects.a comprehensive investigation was done to evaluate the allergenic algae in the delhi area. results of 4,000 skin tests performed on 400 patients suffering from naso-bronchial allergy and 300 skin tests on 30 healthy persons with 10 common algae isolated from the dehli atmosphere are presented and compared. positive skin reactions (1+ to 3+) to algae extracts ranged from 25.7% with lyngbya major extract to 1.7% with oscillatoria simplicissima extract; in healthy non-allergic volunteers there were ...1979434586
microbial succession and mineral leaching in an artificial coal artificial pyritic coal spoil was prepared and examined over a period of 1.5 years for changes in the population of various physiological varieties of bacteria and also for mineral leaching. heterotrophic bacteria were the first to dominate the spoil, acquiring a population of 10(7) cells per g within 2 weeks. bacteria capable of utilizing choline sulfate as the sole source of energy comprised approximately 1% of the total heterotrophic bacteria. sulfur-oxidizing autotrophic bacteria (thiobac ...1978736542
facultative anoxygenic photosynthesis in the cyanobacterium oscillatoria isolate from h2s-rich layers of the solar lake, the cyanobacterium oscillatoria limnetica, exhibits both oxygenic and anoxygenic photosynthesis. it can use na2s as an electron donor for co2 photoassimilation (photosystem i supplies the energy) in the presence of 3-(3,4-dichlorophenyl)-1,1-dimethylurea or 700-nm light. a stoichiometric ratio of approximately 2 is observed between the na2s consumed and the photoassimilated co2. the anoxygenic phototrophic capability of this cyanobacterium expla ...1975808537
[monstrous growth in pseudomicrothorax dubius (ciliatea, holotrichia, trichostomatida) in consequence of effect of plastic on the feed alga (oscillatoria formosa) (author's transl)]. 1975809809
autochton blue-green alga (oscillatoria sp.) with high protein content and self-aggregation properties. 1976822900
[consumption and nutritional contribution of the blue algae (oscillatoria platensis) among some populations of kanem (tchad)].two food surveys determined the areas of consumption and the ethnic groups involved as well as the way and motivations for using spirulina algae (oscillatoria platensis) in chad. more than 400 homes were visited in february (dry season) and october (end of the rainy season and period after the millet harvest). consumption is limited to a restricted proportion of the population in the kanem region. among the kanembu group (the leading consumers of spirulina), frequencies of use vary between one a ...1975824994
[ultrastructure of spirulina in comparison with oscillatoria].the following filamentous blue-green algae: s. platensis, s. platensis f. minor and s. princeps were observed under electronmicroscope and were compared with two species of the related genera oscillatoria: o. rubescens and o. chalybea. from our research it was resulted that spirulina is characterized by a more dense cellular content and by a more wide thylakoid system. the protoplasm shows various types of inclusions: polyglucan granules, lipid droplets, eyanophycin granules, polyhedral bodies, ...1976827871
marine oscillatoria (trichodesmium): explanation for aerobic nitrogen fixation without heterocysts.nitrogen fixation in marine oscillatoria appears to be associated with differentiated cells located in the center of the colony. these central cells exhibit reduced pigmentation relative to peripherally located cells and do not incorporate 14co2 in photosynthesis. central cells apparently do not produce o2 which would deactivate nitrogenase. when central cells are exposed to o2 via disruption of the colonies, n2 fixation (acetylene reduction) decreases sharply even though individual trichomes re ...19761257749
identification of anatoxin-a in benthic cyanobacteria (blue-green algae) and in associated dog poisonings at loch insh, deaths occurred in 1990 and 1991 after the animals drank water containing blooms of benthic cyanobacteria along the shoreline of loch insh, scotland. signs of poisoning in the affected animals and the high neurotoxicity of bloom extracts in laboratory bioassays indicated acute poisoning due to cyanobacterial neurotoxin(s). the neurotoxic blooms consisted largely of benthic oscillatoria species which were also observed in the stomach contents of the poisoned dogs. stomach contents were also n ...19921440622
microcystin-like toxins in different freshwater species of january and september of 1989 and march 1990 blooms of oscillatoria rubescens, oscillatoria tenuis and oscillatoria mougetii were found in lake simbirizzi and lake flumendosa in sardinia, and in lake san puoto in the lazio region of italy. by using different extraction methods and hplc analysis, two microcystin-like toxins (rr-like and yr-like), similar to some of the toxic compounds produced by the cyanophycea microcystis aeruginosa, were detected in these blooms.19921440636
the cyanobacterium oscillatoria erythraea--a potential source of toxin in the ciguatera food-chain.a compound lethal to mice (i.p.) was extracted from samples of oscillatoria erythraea, four species of mollusc and one species of molluscivorous teleost collected from the south-east coast of queensland, australia, during and shortly after o. erythraea blooms. the compound was chemically indistinguishable from ciguatoxin (ctx) on the basis of solvent extraction and partitioning and silicic acid chromatography. residues derived from toxic samples elicited signs of intoxication, death and histopat ...19921493884
evidence for an nih shift in oxidation of naphthalene by the marine cyanobacterium oscillatoria sp. strain jcm.the marine cyanobacterium oscillatoria sp. strain jcm oxidized naphthalene predominantly to 1-naphthol. experiments with [1-2h]naphthalene and [2-2h]naphthalene indicated that 1-naphthol was formed with 68 and 74% retention of deuterium, respectively. no significant isotope effect was observed when the organism was incubated with a 1:1 mixture of naphthalene and [2h8]naphthalene. the results indicate that 1-naphthol is formed through a naphthalene 1,2-oxide intermediate, which rearranges spontan ...19921599253
[investigations of the immunomodulatory effect of cyanobacterial extracts].resulting from the knowledge that cyanobacteria (blue-green algae) are able to produce pharmacologically active substances the aqueous extracts from several cyanobacteria species and strains (microcystis aeruginosa, synechocystis aquatilis, oscillatoria redekei, anabaena flos-aque, aphanizomenon flos-aquae, oscillatoria rubescens, oscillatoria tenuis) were tested for their immunomodulating activity. extracts from oscillatoria redekei 051, oscillatoria tenuis 01 and synechocystis aquatilis 428 ca ...19911801597
sulfide-induced sulfide-quinone reductase activity in thylakoids of oscillatoria limnetica.sulfide-dependent partial electron-transport reactions were studied in thylakoids isolated from cells of the cyanobacterium oscillatoria limnetica, which had been induced to perform sulfide-driven anoxygenic photosynthesis. it was found that these thylakoids have the capacity to catalyze electron transfer, from sulfide to externally added quinones, in the dark. assay conditions were developed to measure the reaction either as quinone-dependent sulfide oxidation (colorimetrically) or as sulfide-d ...19911898723
[the immunosuppressive action of contents of oscillatoria redekai van goor (cyanphyceae)]. 19911907011
effects of light, temperature, nitrate, orthophosphate, and bacteria on growth of and hepatotoxin production by oscillatoria agardhii strains.the effects of bacteria, temperature, light, nitrate, and orthophosphate on growth of and hepatotoxin (desmethyl-3-microcystin-rr) production by oscillatoria agardhii strains were studied under laboratory conditions. strains were cultivated in z8 medium under continuous illumination. growth was determined by measuring dry weight and chlorophyll a, while toxin was analyzed by high-performance liquid chromatography. two of the three toxic cultures studied produced more toxins in axenic than in non ...19902125814
light-dependent delta mu na-generation and utilization in the marine cyanobacterium oscillatoria brevis.light-dependent na+ and h+ transports, membrane potential (delta psi) and motility have been studied in the cells of the marine cyanobacterium oscillatoria brevis. in the presence of a protonophorous uncoupler, carbonyl cyanide-m-chlorophenylhydrazone, the intracellular na+ level is shown to increase in the dark and decrease in the light. the na+/h+ antiporter, monensin, stimulates the dark cccp-dependent [na+]in increase and abolishes the light-dependent [na+]in decrease. na+ ions are necessary ...19902171990
sulfide induction of synthesis of a periplasmic protein in the cyanobacterium oscillatoria limnetica.two proteins which may play a role in the induction of anoxygenic photosynthesis in oscillatoria limnetica have been demonstrated by comparing the pattern of labeling during pulses of [35s]methionine of cells incubated under inducing conditions [anaerobic conditions plus 3-(3,4-dichlorophenyl)-1,1-dimethylurea, light, and sulfide) with that of cells incubated under noninducing conditions (without sulfide). the major inducible protein has an apparent molecular mass of 11.5 kilodaltons and is asso ...19892492513
occurrence of the hepatotoxic cyanobacterium nodularia spumigena in the baltic sea and structure of the toxin.water blooms formed by potentially toxic species of cyanobacteria are a common phenomenon in the baltic sea in late summer. twenty-five cyanobacterial bloom samples were collected from open and coastal waters of the baltic sea during 1985 to 1987, and their toxicity was determined by mouse bioassay. all of 5 bloom samples from the southern baltic sea, 6 of 6 from the open northern baltic sea (gulf of finland), and 7 of 14 finnish coastal samples were found to contain hepatotoxic cyanobacteria. n ...19892506812
structure and toxicity of a peptide hepatotoxin from the cyanobacterium oscillatoria agardhii.a peptide hepatotoxin was isolated by reversed phase liquid chromatography from the cyanobacterium oscillatoria agardhii and characterized structurally and toxicologically. amino acid analyses, proton nuclear magnetic resonance and fast atom bombardment mass spectrometry showed that the toxin is a cyclic heptapeptide (mol. wt 1023.5) with the structure cyclo-(ala-arg-asp-arg-adda-glu-n-methyldehydroala) (adda: 3-amino-9-methoxy-2,6,8-trimethyl-10-phenyldeca-4,6-dienoic acid). in mice the toxic e ...19892508270
sulfide-dependent photosynthetic electron flow coupled to proton translocation in thylakoids of the cyanobacterium oscillatoria limnetica.light-induced proton translocation coupled to sulfide-dependent electron transport has been studied in isolated thylakoids of the cyanobacterium oscillatoria limnetica. the thylakoids are obtained by osmotic shock of washed spheroplasts, prepared with glycine-betaine as the osmotic stabilizer. 13c nmr studies suggests that betaine is the major osmoregulator in o. limnetica. thylakoid preparations obtained from both sulfide-induced anoxygenic cells and noninduced oxygenic cells are capable of pro ...19872827581
toxicity studies with the blue-green alga oscillatoria agardhii from two eutrophic norwegian lakes. 19852869653
use of freshly prepared rat hepatocytes to study toxicity of blooms of the blue-green algae microcystis aeruginosa and oscillatoria agardhii.extracts from blue-green algal blooms (microcystis aeruginosa and oscillatoria agardhii) from different lakes in southeastern norway were tested for toxicity toward freshly prepared rat hepatocytes. the toxicity effects were scored by means of morphological studies of the cells and by measuring leakage of the enzyme lactate dehydrogenase (ldh) from the cells. the results with the hepatocytes correspond well with results from the traditional mouse bioassay, concerning both ability to distinguish ...19863095554
guidance of cytophaga sp. strain u67 gliding on the sheaths of oscillatoria princeps.individual cells of cytophaga sp. strain u67 glided in helical patterns on the surface of sheaths deposited by the cyanobacterium oscillatoria princeps. possible bases for the helical substructure of the sheath are discussed.19853921528
effects of oscillatoria agardhii-toxins on blood pressure and isolated organ preparations. 19853938183
a sequence analysis of 5 s rrna from the blue-green alga oscillatoria tenuis and a comparison of blue-green alga 5 s rrna with those of bacterial and eukaryotic origin. 19744214272
c-phycocyanin from oscillatoria agardhil. i. some molecular properties. 19724624163
on oscillatoria redkei van goor. 19694986659
[importance of oscillatoria formosa bory in the laboratory culture of mollusc vectors of schistosoma haematobium]. 19685695201
virus-like structures in the cells of the blue-green alga, oscillatoria princeps. 19655866073
[on the characterization of gas vacuoles of the blue alga oscillatoria rubescens]. 19665985349
studies on the utilization of octane by algae.the objective of this study was to investigate the influence of various octane concentrations on a mixed culture of scenedesmus obliquus, chlorella vulgaris and oscillatoria sp. and the ability of these algae to utilize octane as the sole source of organic carbon in the medium. the same investigations were carried out for a mixed culture consisting of algae and the strain acinetobacter calcoaceticus, and for a. calcoaceticus alone. the results obtained show that all of the strains under study, b ...19836196951
some aspects of the ecophysiology of cyanobacteria.fresh waters rich in nutrients often show mass development of cyanobacteria. the kind of cyanobacteria to be found depends on the properties of the lake. in non-stratified shallow lakes, the most common species oscillatoria agardhii. in stratified lakes, cyanobacteria can be found in restricted zones of the deeper part of the lakes, and always possess cells with very active gas vacuoles. the most common species are microcystis aeruginosa, oscillatoria agardhii var isothrix, oscillatoria "var red ...19836416128
[photosynthetic oxygen evolution and co2 photoassimilation by cyanobacteria that form water-bloom spots in a sulfur spring with a high sulfide content].cyanobacteria belonging mainly to the genera anabaena and oscillatoria were isolated from water-bloom spots of a sulfur spring in staraya matsesta. their suspensions evolved o2 at a rate of 6--8 nm/min per 1 mg of dry cell weight at an intensity of solar radiation being 60--75 mv/cm2 per 1 sec. the cells were also capable of co2 photoassimilation in the presence of solfide at a rate of 10(-4) mg c per mg per hour. dcmu at a concentration of 10(-5) m completely inhibited o2 evolution and inhibite ...19806446661
oxidation of biphenyl by the cyanobacterium, oscillatoria sp., strain jcm.the oxidation of biphenyl by cyanobacterium, oscillatoria sp., strain jcm was studied. the organism grown photoautotrophically in the presence of biphenyl oxidized biphenyl to form 4-hydroxybiphenyl. the structure of the metabolite was elucidated by ultraviolet and mass spectra and shown to be identical to authentic 4-hydroxybiphenyl. in addition this metabolite had properties identical to 4-hydroxybiphenyl when analyzed by thin-layer and high-pressure liquid chromatography. experiments with [14 ...19806769418
gliding motility in aphanothece halophytica: analysis of wall proteins in mot mutants.the unicellular cyanobacterium aphanothece halophytica (pcc 7418) is motile, and spontaneous nonmotile (mot) mutants accumulate when the organism is subcultured. analysis of mot mutants suggests that a glycoprotein in the cell wall is involved in the motility mechanism. proteins from the wall fraction of the wild type and five mot clones were analyzed by gradient sodium dodecyl sulfate-acrylamide gel electrophoresis. four clones were similar to the wild type, and one clone, mot-3, was missing a ...19816793555
production and properties of cyanobacterial endotoxins.lipopolysaccharides (lps) were isolated from four species of cyanobacteria (anabaena flos-aquae utex 1444. a. cylindrica, oscillatoria tenuis, and o. brevis) frequently occurring in drinking-water supplies. the cyanobacterial lps contained glucose, xylose, mannose, and rhamnose, but differed from the lps derived from most gram-negative bacteria because of the variable presence of 2-keto-3-deoxyoctonate, heptose, galactose, and glucosamine. cyanobacterial lipid a is characterized by long-chain sa ...19826798930
biotransformation and toxicity of aniline and aniline derivatives of cyanobacteria.agmenellum quadruplicatum strain pr-6 and oscillatoria sp. strain jcm grown photoautotrophically in the presence of aniline metabolized the aromatic amine to formanilide, acetanilide and p-aminophenol. the metabolites were isolated by either thin-layer, gas-liquid or high pressure liquid chromatography and identified by comparison of their chromatographic, ultraviolet absorbance and mass spectral properties with those of authentic compounds. the toxicity of aniline derivatives towards agmenellum ...19816800332
electrical nature of the taxis signal in cyanobacteria.electrical events after a light-dark stimulus were studied in the multicellular organism phormidium uncinatum. normally, such a stimulus causes the gliding trichome to reverse direction. by directing a large light spot on the end of a batch of trichomes and then switching it off, we achieved synchronization of the trichomes, since the "head" is much more sensitive than the "tail." the abrupt disappearance of a uniform light produced a depolarization wave which initiated at the head, as registere ...19826801020
[identification of volatile metabolites produced by blue-green algae, oscillatoria splendida, o. amoena, o. geminata and aphanizomenon sp]. 19816820045
microbial degradation of thidiazuron and its photoproduct.degradation of the cotton defoliant thidiazuron and its photoproduct photothidiazuron by soil and thirteen species of microorganisms was examined. aspergillus versicolor, torula rosea, and flavobacter sp. were most active in degrading thidiazuron. unknown water-soluble metabolites and phenylurea were the major products. a. versicolor and penicillium cyclopium were most active in degrading photothidiazuron. 4-hydroxyphenylphotothidiazuron was the major organosoluble product formed by a. versicolo ...19827073312
evolutionary affiliation of the marine nitrogen-fixing cyanobacterium trichodesmium sp. strain nibb 1067, derived by 16s ribosomal rna sequence analysis.the 16s rrna sequence of trichodesmium sp. strain nibb 1067 was determined and used for the construction of a distance tree and bootstrap analysis. the tree shows that, among the available cyanobacterial 16s rrna sequences, trichodesmium nibb 1067 has oscillatoria pcc 7515 as its closest relative, presenting 94.9% of sequence similarity with the latter strain. this is in contrast to a difference of 9 mol% g+c in mean genomic dna base composition between the two organisms. nevertheless, the genot ...19947522848
the role of phosphatidylglycerol as a functional effector and membrane anchor of the d1-core peptide from photosystem ii-particles of the cyanobacterium oscillatoria chalybea.the intrinsic polypeptide d1, isolated from photosystem (ps) ii-particles of the cyanobacterium oscillatoria chalybea, was obtained by electroelution and fractionated extraction with organic solvents. purification was demonstrated by western blotting and amino acid sequencing. by carrying out d1-immunization in rabbits a polyclonal monospecific d1-antiserum was obtained. for the qualitative characterization of d1 as a lipid-binding peptide, the effect of the lipids phosphatidylglycerol (pg), mon ...19957546031
fine structure of elongate polyhedral bodies (carboxysomes) in two oscillatoria (cyanophyceae) isolates.polyhedral bodies (carboxysomes) in two oscillatoria isolates were of two morphological forms. some were typical polygonal bodies with many straight sides and were approximately 0.2 micron in diameter. most of the bodies in these isolates were elongate being up to 2.6 microns in length, 33 nm in width and approximately 0.1 micron in depth. some of the bodies were polygonal with an elongate extension. all of the bodies were limited by a membrane monolayer of approximately 20 a. the function of po ...19947837992
characterization of metal-binding bioflocculants produced by the cyanobacterial component of mixed microbial mats.mixed-species microbial mats that were dominated by the cyanobacterium oscillatoria sp. and contained heterotrophic and purple autotrophic bacteria were constructed for specific bioremediation applications. when the mats were challenged with metals, production and secretion of metal-binding extracellular polysaccharide bioflocculants were observed. the concentration of these negatively charged polysaccharides was correlated with the removal of manganese from the water column beneath a surface mi ...19948074512
purification and characterization of sulfide-quinone reductase, a novel enzyme driving anoxygenic photosynthesis in oscillatoria enzyme catalyzing sulfide quinone oxido-reduction (e.c.1.8.5.'.; sqr) has been purified in an active form, from thylakoids of the cyanobacterium oscillatoria limnetica. it is composed of a single polypeptide of about 57 kda. the catalytic activity of the purified enzyme is similar to the membrane-bound form in its kinetic parameters: apparent km for sulfide equals 8 microm; vmax of 100-150 mumol of plastoquinone-1 reduced/mg protein/h; quinone-substrate specificity; differential sensitivity t ...19948119908
distribution of types of microbial mats at the ebro delta, spain.the distribution and types of microbial mats of the ebro delta (catalonia, spain) are described. the studied area is la banya spit, formed by a narrow sand bar and a peninsula, located south of the main body of the ebro delta. sea water can penetrate into the back shore through channel inlets, cutting the steeper coastal barrier of the open sea side of the spit or through the complex drainage channel system of the low-energy beaches in the inner alfacs bay. sea water can stay in the back shore a ...19938155846
hepatotoxin (microcystin) and neurotoxin (anatoxin-a) contained in natural blooms and strains of cyanobacteria from japanese freshwaters.amounts of hepatotoxic microcystin and neurotoxic anatoxin-a were estimated in natural blooms and strains of cyanobacteria from freshwaters in japan. a simultaneous analysis method of anatoxin-a and microcystin was applied to natural bloom samples, which has been dominated by several species and the strains of cyanobacteria which produced simultaneously both toxins. the natural blooms examined in the present study were mainly composed of anabaena and oscillatoria, but most also contained microcy ...19938167957
isolation and identification of eight microcystins from thirteen oscillatoria agardhii strains and structure of a new microcystin.microcystins (cyclic heptapeptide hepatotoxins), isolated from 13 freshwater oscillatoria agardhii strains from eight different finnish lakes by high-performance liquid chromatography, were characterized by amino acid analysis, fast atom bombardment mass spectrometry (fabms), and tandem fabms (fabms/collisionary-induced dissociation/ms). all strains produced two to five different microcystins. in total, eight different compounds, of which five were known microcystins, were isolated. the known co ...19938357254
nucleotide sequence of the nar beta gene encoding assimilatory nitrate reductase from the cyanobacterium oscillatoria chalybea.the nucleotide sequence of the structural gene of nitrate reductase (n ar beta) has been determined from the filamentous, non-heterocystous cyanobacterium oscillatoria chalybea. the nar beta gene encodes a protein of 737 amino acid residues, which shows 61% identity to nitrate reductase of the unicellular cyanobacterium synechococcus sp. pcc 7942 and only weak homologies to different bacterial molybdoenzymes, such as nitrate reductases or formate dehydrogenases.19968605243
[studies on microcystin contents in different drinking water in highly endemic area of liver cancer].in order to study the pollution with microcystin (mc), a potent liver promoter, in drinking water in highly endemic area of liver cancer, cyanobacteria and its product mc were detected in drinking water in haimen, jiangsu province during 1992 to 1993. oscillatoria was identified as the most common species of cyanobacteria in pond-ditch and river water in haimen, jiangsu province, which can produce mc. mc was detected in two of 65 water samples with high performance liquid chromatography, liquid ...19968758860
toxic effects of blooms of marine species of oscillatoriales on farmed prawns (penaeus monodon, penaeus japonicus) and brine shrimp (artemia salina).benthic and planktonic blooms of species of oscillatoriales coincided with mortalities of penaeus monodon during four episodes at australian prawn farms. oscillatoria corakiana was the dominant planktonic species at 65-90,000 cells/ml, but spirulina sp., lyngbya sp., oscillatoria sp. and nodularia sp. were also identified from the water column, benthic layers or surface mats. the levels and variety of vibrionaceae in prawn tissue, suggest that mortalities were caused by secondary infections of b ...19968875773
a novel monogalactosylacylglycerol with inhibitory effect on platelet aggregation from the cyanophyceae oscillatoria rosea.(2s)-1-o-palmitoyl-3-o-beta-d-galactopyranosylglycerol, isolated from the marine alga oscillatoria rosea, inhibited platelet aggregation induced by u46619, a thromboxane a2 analogue. its structure was elucidated by spectroscopic analysis and chemical evidence.19968882435
[red tides in méxico: a review].with the purpose to make a review on the red tides occurence at mexican coasts, previous studies were analyzed. dinoflagellates seem to be the main cause of toxic events mainly gonyaulax polygramma, gymnodinium catenatum, pyrodinium bahamense var. compressum and ptychodiscus brevis. there are other species which cause red tides but are not toxic. they are: mesodinium rubrum, gonyaulax triacantha, noctiluca scintillans, prorocentrum dentatum, gymnodinium splendens, g. sanguineum, ceratium tripos ...19958900570
phylogenetic relationships of nonaxenic filamentous cyanobacterial strains based on 16s rrna sequence order to determine the nearly complete 16s rrna gene sequences of cyanobacteria originating from nonaxenic cultures, a cyanobacterium-specific oligonucleotide probe was developed to distinguish polymerase chain reaction (pcr) products of the cyanobacterial rrna operons from those resulting from amplification of contaminating bacteria. using this screening method the 16s rrna genes of four nonaxenic filamentous cyanobacterial strains belonging to the genera leptolyngbya and oscillatoria were c ...19968919871
a novel inhibitor of platelet aggregation from the cyanophyceae oscillatoria rosea (nies-208)(2s)-1-o-palmitoleyl-3-o-beta-d-galactopyranosylglycerol was isolated from the marine alga oscillatoria rosea. it caused a concentration-dependent inhibition of platelet aggregation induced by u46619. its structure was elucidated by spectroscopic analysis and chemical evidence.19968923815
effects of 2,4-dichlorophenoxyacetic acid on kentucky algae: simultaneous laboratory and field toxicity testings.2,4-d was applied to a cove in kentucky lake which was highly infested with myriophllum spicatum (eurasian watermilfoil). effects of 2,4-d on nontarget algal communities were monitored concurrently in the field and in laboratory microcosms for eight days. results indicated that indirect effects of water temperature and increased nutrient concentrations due to lysis in milfoil plants may be more important in the field community dominated by chlorophyta, pyrrhophyta, and bacillariophyta. 2,4-d app ...19968975831
oscillapeptin g, a tyrosinase inhibitor from toxic oscillatoria agardhii.oscillapeptin g, a tyrosinase inhibitor, was isolated from the freshwater toxic cyanobacterium oscillatoria agardhii. the structure was determined to be 1 by chemical degradation and 2d nmr analyses.19968984160
sulfide-quinone reductase from rhodobacter capsulatus. purification, cloning, and expression.a sulfide-quinone oxidoreductase (sqr, ec 1.8.5.'.) has been purified to homogeneity from chromatophores of the non-sulfur purple bacterium rhodobacter capsulatus dsm 155. it is composed of a single polypeptide with an apparent molecular mass of about 55 kda, exhibiting absorption and fluorescence spectra typical for a flavoprotein and similar to the sqr from the cyanobacterium oscillatoria limnetica. from n-terminal and tryptic peptide sequences of the pure protein a genomic dna clone was obtai ...19979092526
structure and composition of freshwater microbial mats from a sulfur spring ("font pudosa", ne spain).different types of microbial mats developing on the wall on a non-thermal sulfur freshwater spring have been studied. both, light and electron microscopy as well as hplc analysis of photosynthetic pigments revealed their structure and composition. prokaryotic chlorophylls and carotenoids helped in the taxonomical assignment of the main photosynthetic groups. "inverted position" mats (mat-i) were dominated by chromatiaceae; they were located closed to the water outlets (0.3 mm sulfide). "normal p ...19979106181
microcystins in slovene freshwaters (central europe)--first report.cyanobacterial blooms are frequent in the north-eastern region of slovenia, where the agricultural activities are intensive, resulting in higher water eutrophication. in a two year monitoring program we identified eighteen blooms of cyanobacteria, fifteen being hepatotoxic by intraperitoneal mouse bioassay. the approximate ld100 varied from 50-1000 mg/kg (dry cell weight/animal weight) and gross pathological signs were characteristic of cyanobacterial hepatotoxins. frequently the blooms were dom ...19979131592
oscillatoria anguistissima: a promising cu2+ biosorbent.oscillatoria anguistissima rapidly adsorbs cu2+ from aqueous solution. the adsorption of cu2+ followed freundlich isotherm, and the amount of cu2+ removed from solution increased with increasing cu2+ concentration. the adsorption is ph dependent, and maximum cu2+ removal occurs at ph 5. of the various pretreatments, hcl treatment of the biomass increased the capacity for cu2+ removal. presence of mg2+ and ca2+ resulted in decline in the cu2+ adsorption capacity of oscillatoria cells. this specie ...19979236296
anatoxin-a in irish freshwater and cyanobacteria, determined using a new fluorimetric liquid chromatographic method.a new sensitive high-performance liquid chromatographic (hplc) method was used to determine anatoxin-a in freshwater, following blooms of cyanobacteria (blue-green algae). anatoxin-a was converted into a highly fluorescent derivative using 4-fluoro-7-nitro-2,1,3-benzoxadiazole and hplc analysis gave good linear calibrations even at low concentration ranges (1-10 micrograms/liter, r = 0.997). the detection limit for anatoxin-a was 0.02 ng/ml, and this new hplc method should prove useful for the r ...19979241789
new acylated sulfoglycolipids and digalactolipids and related known glycolipids from cyanobacteria with a potential to inhibit the reverse transcriptase of hiv-1.five novel diacylated sulfoglycolipids (1-5) were isolated from the cyanobacterium scytonema sp. (tau strain sl-30-1-4) and four novel acylated diglycolipids (6-9) were isolated from the cyanobacterium oscillatoria raoi (tau strain il-76-1-2). these two groups of glycolipids and related known glycolipids isolated from these two and three other strains of cyanobacteria, phormidium tenue (tau strain il-144-1), o. trichoides (tau strain il-104-3-2), and o. limnetica (tau strain ng-4-1-2), were foun ...19979428159
sensitive determination of anatoxin-a, homoanatoxin-a and their degradation products by liquid chromatography with fluorimetric detection.cyanobacterial neurotoxins have been implicated in animal deaths resulting from drinking contaminated water. anatoxin-a (an) and homoanatoxin-a (hman) have previously been analysed using high-performance liquid chromatography (hplc) with uv detection, but this procedure is insufficiently sensitive and is subject to interferences. a sensitive fluorimetric (fl) method for determining an was recently developed using derivatisation with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (nbd-f) and this has been ...19989542136
selective growth inhibition of the musty-odor producing cyanobacterium oscillatoria cf. chalybea by natural compounds. 19989557207
microbial production of hydrogen: an overview.production of hydrogen by anaerobes, facultative anaerobes, aerobes, methylotrophs, and photosynthetic bacteria is possible. anaerobic clostridia are potential producers and immobilized c. butyricum produces 2 mol h2/mol glucose at 50% efficiency. spontaneous production of h2 from formate and glucose by immobilized escherichia coli showed 100% and 60% efficiencies, respectively. enterobactericiae produces h2 at similar efficiency from different monosaccharides during growth. among methylotrophs, ...19989561824
studies on the phytoplankton populations and physico-chemical conditions of treated sewage discharged into lake manzala in egypt.over a full year, the phytoplankton populations and physico-chemical conditions of treated sewage discharged into lake manzala in egypt were investigated. sixty-seven species of algae were identified, 18 cyanophyta (cyanobacteria), 19 chlorophyta, 21 bacillariophyta, 6 euglenophyta, 2 cryptophyta and one species pyrrhophyta. nitzschia (6 spp.), scenedesmus (6 spp.), navicula (4 spp.), oscillatoria (4 spp.) and euglena (4 spp.) were the most common genera. a remarkable seasonal variation in speci ...19989579343
first identification of microcystins in irish lakes aided by a new derivatisation procedure for electrospray mass spectrometric analysis.recent animal and bird deaths at several lakes in ireland were indicative of possible cyanobacterial poisoning. using protein phosphatase inhibition assays, microcystins (mcs) were identified in extracts of cyanobacteria from several lakes at concentrations ranging from 1.6 to 168 micrograms/g. this is the first report of mcs in irish freshwaters. the protein phosphatase inhibition assay was used to screen fractions during hplc purification of the mcs in cyanobacteria (anabaena and oscillatoria) ...19979615313
two (z)-dehydrobutyrine-containing microcystins from a hepatotoxic bloom of oscillatoria agardhii from soulseat loch, scotland.two (z)-dehydrobutyrine(dhb)-containing microcystins, [d-asp3, (z)-dhb7]microcystin-htyr (1) and [d-asp3, (z)-dhb7]microcystin-lr (2), were isolated from a hepatotoxic bloom of the cyanobacterium oscillatoria agardhii from a freshwater lake in scotland. the geometrical structure of the dhb units in the microcystins was determined as z on the basis of noe and roesy experiments.19989644085
algicidal effects of a novel marine pseudoalteromonas isolate (class proteobacteria, gamma subdivision) on harmful algal bloom species of the genera chattonella, gymnodinium, and heterosigma.during a bacterial survey of the huon estuary in southern tasmania, australia, we isolated a yellow-pigmented pseudoalteromonas strain (class proteobacteria, gamma subdivision), designated strain y, that had potent algicidal effects on harmful algal bloom species. this organism was identified by 16s rrna sequencing as a strain with close affinities to pseudoalteromonas peptidysin. this bacterium caused rapid cell lysis and death (within 3 h) of gymnodinoids (including gymnodinium catenatum) and ...19989687434
uv-a-induced expression of groel in the uv-a-resistant marine cyanobacterium oscillatoria sp. nkbg 091600.the authors have examined the response to uv-a irradiation of the uv-a-resistant marine cyanobacterium oscillatoria sp. nkbg 091600, which produces the uv-a-absorbing compound biopterin glucoside. the expression of a 60 kda protein was markedly induced at 500 min after uv-a irradiation. this protein was identified by n-terminal amino acid sequence analysis as groel. northern blot analysis demonstrated that groel synthesis was controlled by uv-a at the transcriptional level. a circe element and a ...199910220174
toxicity in a water column following the stratification of a cyanobacterial population development in a calm lake.cyanobacteria, including the genera anabaena, aphanizomenon, microcystis, and oscillatoria, are known to release or have the potential to release phycotoxins into the water. indeed, there are documented cases of both animal and human intoxication. data obtained from field observations and laboratory experiments demonstrate a correlation between the development of the cyanobacterial population and the level of phycotoxin present in the water, though it should be noted that not all cyanobacterial ...199910335601
anabaenopeptins g and h, potent carboxypeptidase a inhibitors from the cyanobacterium oscillatoria agardhii (nies-595).anahaenopeptins g (1) and h (2) were isolated from the cultured cyanobacterium oscillatoria agardhii (nies-595) as potent carboxypeptidase a (cpa) inhibitors. the gross structure of 1 and 2 were established by spectroscopic analysis including the 2d nmr technique and the absolute configurations of 1 and 2 were determined by the spectral and chemical methods. 1 and 2 inhibited cpa with ic50's of 0.0018 and 3.4 microg/ml, respectively.199910340607
prenylagaramides a and b, new cyclic peptides from two strains of oscillatoria agardhiiprenylagaramides a (1) and b (2), two new cyclic peptides, were isolated from the cultured cyanobacteria oscillatoria agardhii (nies-205) and o. agardhii (nies-596), respectively. the structures of 1 and 2, which both contain a rare o-prenyltyrosine (ptyr) unit, were established as cyclo(-ptyr1-gly2-thr3-gly4-glu5-phe6-phe7-asn8-pro9-) and cyclo(-ptyr1-leu2-tyr3-pro4-ile5-asn6-pro7-), respectively, by spectroscopic analysis and chemical degradation.199910346961
detritus-dependent development of the microbial community in an experimental system: qualitative analysis by denaturing gradient gel electrophoresis.correlations between the biomass of phytoplankton and the biomass of bacteria and between the biomass of bacteria and the biomass of protozoans suggest that there is coupling between these compartments of the "microbial loop." to investigate this coupling on the species level, bacteria and protozoans from untreated lake water inocula were allowed to grow on detritus of the green alga ankistrodesmus falcatus or the cyanobacterium oscillatoria limnetica in continuous-flow systems for 1 month. dena ...199910347030
sorption and desorption of cobalt by oscillatoria anguistissima.oscillatoria anguistissima rapidly adsorbs appreciable amounts of cobalt from the aqueous solutions within 15 min of initial contact with the metal solution. o. anguistissima showed a high sequestration of cobalt at low equilibrium concentrations, and it followed the freundlich model of adsorption. the adsorption is a strongly ph-dependent and temperature-independent phenomenon. the presence of mg2+ and ca2+ (100-200 ppm) resulted in decline in co2+ adsorption capacity of oscillatoria biomass. s ...199910387117
the diversity of gas vesicle genes in planktothrix rubescens from lake zürich.part of the gas vesicle gene cluster was amplified by pcr from three strains of planktothrix rubescens isolated from lake zürich, switzerland. each contains multiple alternating copies of gvpa and gvpc. all of the gvpa sequences in the different strains are identical. there are two types of gvpc: gvpc20, of length 516 bp, encodes a 20 kda protein of 172 amino acid residues (whose n-terminal amino acid sequence is homologous with the sequence of gvpc in planktothrix [oscillatoria] agardhii); gvpc ...199910537197
beta-cyanoalanine production by marine bacteria on cyanide-free medium and its specific inhibitory activity toward screening the culture broth of marine bacteria collected at yap (micronesia), palau (belau), and okinawa (the southwest islands of japan) for antimicroalgal activity, 37 out of 2,594 bacterial isolates tested were found to produce anticyanobacterial substances against oscillatoria amphibia nies-361. one strain, c-979, identified as a vibrio sp., was selected and cultured in 2.4 liters of marine broth 2216 to identify the bioactive compound produced by the strain. the purified very hydrophilic ...200010653741
9,10-anthraquinone reduces the photosynthetic efficiency of oscillatoria perornata and modifies cellular inclusions.the natural compound 9,10-anthraquinone was found to inhibit the growth of the musty odor-producing cyanobacterium oscillatoria perornata at a low concentration (1 µm) in previous laboratory studies. in this study, the mode of action of 9,10-anthraquinone was investigated by observing ultrastructural changes in o. perornata and by monitoring chlorophyll fluorescence as an indicator of photosynthetic efficiency. results indicate that 9,10-anthraquinone inhibits photosynthetic electron transport, ...200010777450
purification and characterization of a novel lectin from a freshwater cyanobacterium, oscillatoria the survey of 14 species of laboratory-cultured cyanobacteria for hemagglutinins, we newly detected the activity in two species, oscillatoria agardhii, strain nies-204, and phormidium foveolarum, strain nies-503. from the extract of o. agardhii, which showed the highest activity with trypsin-treated erythrocytes of rabbit, a lectin was purified to homogeneity by the combination of precipitation with (nh4)2so4, gel filtration, hydrophobic chromatography and reverse phase chromatography. the pu ...200010817903
use of protein phosphatase inhibition assay to detect microcystins in donghu lake and a fish pond in china.seasonal variations in the level of total microcystins in water samples collected from donghu lake and a fish pond in wuhan, china, were studied between march 1995 and february 1996 using a protein phosphatase inhibition assay involving a radioactive 32p-labelled substrate. the assay is highly reliable and repeatable, and is probably the most sensitive assay for microcystin detection to date. results of the survey indicated the presence of microcystins in the water samples, and the concentration ...200010819179
cyanobacterial sulfide-quinone reductase: cloning and heterologous expression.the gene encoding sulfide-quinone reductase (sqr; e.c.1.8.5.'), the enzyme catalyzing the first step of anoxygenic photosynthesis in the filamentous cyanobacterium oscillatoria limnetica, was cloned by use of amino acid sequences of tryptic peptides as well as sequences conserved in the rhodobacter capsulatus sqr and in an open reading frame found in the genome of aquifex aeolicus. sqr activity was also detected in the unicellular cyanobacterium aphanothece halophytica following sulfide inductio ...200010852862
phototrophs in high iron microbial mats: microstructure of mats in iron-depositing hot springs.chocolate pots hot springs in yellowstone national park are high in ferrous iron, silica and bicarbonate. the springs are contributing to the active development of an iron formation. the microstructure of photosynthetic microbial mats in these springs was studied with conventional optical microscopy, confocal laser scanning microscopy and transmission electron microscopy. the dominant mats at the highest temperatures (48-54 degrees c) were composed of synechococcus and chloroflexus or pseudanaba ...200010858577
[cyanobacteria as indicators of organic contamination].in two costa rican rivers used as receptors for domestic sewage, treated by primary stabilization ponds, were taken a total of 28 samplings located at the pond exit and at three different sites in each river: 100 m before the ponds discharge, at the discharge and 100 m after the discharge. these sampling were done for a five and a half years including dry and rainy seasons. in each sampling site, samples were collected of five different substrates: stones, submerge and semi submerge vegetation, ...199910883326
composition and some biological activities of the essential oil of callicarpa americana (l.).the essential oil profile of callicarpa americana was examined. samples were collected from lafayette county in north central mississippi, and gc-ms data and retention indices were used to identify 67 oil components. humulene epoxide ii (13.9%), alpha-humulene (10.0%), 7-epi-alpha-eudesmol (9.4%), beta-pinene (8.8%), and 1-octen-3-ol (8.5%) were the major components of the steam-distilled oil. the oil was selectively toxic toward the cyanobacterium oscillatoria perornata compared to oscillatoria ...200010898657
optimum conditions for growth in liquid medium of oscillatoria formosa bory used as the principal food in laboratory culture of intermediate hosts for schistosomosis and fasciolosis.the rearing of snails, intermediate hosts of schistosoma haematobium, s. intercalatum, s. bovis and fasciola hepatica is the first step to maintain the life cycle of these parasites in laboratory in order to have biological material for the different studies, namely on the systematic biology and immunodiagnostic of schistosomosis and fasciolosis. according to the traditional method, the alga oscillatoria formosa bory (cyanobacteria), principal food source for the snails, was cultivated in soil e ...200011031760
biochemical characterization of cyanobacterial extracellular polymers (eps) from modern marine stromatolites (bahamas).a range of biochemical characterizations were used to examine the extracellular polymeric secretions (eps) of two cyanobacteria, synechocystis sp. and oscillatoria sp., isolated from marine stromatolites, bahamas. two-dimensional gel electrophoresis was successfully used to fractionate proteins in eps. the results suggest that cyanobacterial eps is composed of a network of macromolecules having different biochemical properties, which may contribute to extracellular functions.200011065277
tolerance of oscillatoria isolates to agrochemicals and pyrethroid components.four isolates of oscillatoria sp from an indian paddy field have been tested for their tolerance to agrochemicals and pyrethroid components. the isolates possessed considerable tolerance to agrochemicals and reduced toxicity of pyrethroid components towards fish.200011218822
long-term compositional changes after transplant in a microbial mat cyanobacterial community revealed using a polyphasic approach.using a polyphasic approach that included microscopy, cultivation and 16s rrna-based cultivation-independent molecular fingerprinting, we compared the cyanobacterial composition of solar lake microbial mats and samples thereof transplanted and maintained in new settings for extended periods of time. significant changes in community composition, with clear replacement of the dominant cyanobacterium, microcoleus chthonoplastes, were detected in all cases. the most dramatic shifts occurred in a sam ...200111225723
cyanobacteria diversity and toxicity in a wastewater treatment plant (portugal).cyanobacteria are common in eutrophic natural waters. being favoured by warm, stable and nutrient-enriched waters they may constitute an important part of the phytoplankton community in wastewater treatment plants (wwtp). the phytoplankton communities of two ponds (facultative and maturation) of the wwtp of esmoriz (north portugal) were studied, with particular importance given to cyanobacteria. mouse bioassays were performed with cyanobacteria samples during some of the blooms and elisa assays ...200111268858
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