| anaphylactic release of a basophil kallikrein-like activity. i. purification and characterization. | these studies describe the ige-mediated relase of a basophil kallikrein-like enzyme that is an arginine esterase and is inhibited by plasma, diisopropylphosphofluoridate, and trasylol. the substrate specificity for the synthetic amino acid ester substrates p-toluenesulfonyl-l-arginien methyl ester, benzoyl-arginine methyl ester, and acetyl-tyrosine methyl ester is similar for the basophil enzyme and plasma kallikrein. the interaction of arginine esterase-active fractions from ion-exchange (deae- ... | 1979 | 37257 |
| the use of hapten-polysaccharide conjugates for the induction of b-cell tolerance involving ige responses. iii. specific tolerance induced by sulphonamide-substituted levan in the guinea pig. | inbred strain 2 and (strain 2 x strain 13)f1 guinea pigs were made allergic by intraperitoneal injections of sulphonamide-chicken gamma-globulin (cgg) conjugates. two sulphonamides were used: 4-sulphanilamide benzoic acid (4-saba) and sulphamethoxazole (smx). 4-saba was coupled to cgg through its carboxylic group and smx was coupled following diazotization of its sulphanilamide group. the anti-4-saba and anti-smx reaginic antibodies formed did not show any cross-reactivity with each other. injec ... | 1979 | 91585 |
| standardization of radioimmunoassay: differences in reactivity of rat iges with antibodies to a monoclonal ige. | | 1979 | 92758 |
| [histamine liberation in the fish meat infected with proteus morgani]. | | 1979 | 92805 |
| activation of glycerol-extracted muscle fibres by uv light [proceedings]. | | 1979 | 92942 |
| are vh framework antigenic determinants expressed on both rabbit b and t lymphocytes? | | 1979 | 93052 |
| reverse transcriptase-associated rnase h. part iv. pyrophosphate does not inhibit rnase h activity of amv dna polymerase. | | 1979 | 93479 |
| occurrence and subcellular localization of glucose 6-phosphatase in bovine thyroid. | in bovine thyroid tissue the glucose 6-phosphatase activity is not entirely due to the presence of an unspecific acid phenylphosphatase nor to beta-glycerophosphatase. this glucose 6-phosphatase is very probably localized within endoplasmic reticulum membranes. it is not a good marker for distribution patterns obtained after differential pelleting. however it can be used as a marker for endoplasmic reticulum membranes after centrifugation in a zonal rotor. | 1979 | 93889 |
| effect of secretin, glucagon and vasoactive intestinal peptide on the hormone-sensitive rat cardiac adenylate cyclase [proceedings]. | | 1979 | 93901 |
| genetic polymorphism and close linkage of two alpha 1-protease inhibitors in horse serum. | two-dimensional electrophoretic analysis of horse serum proteins was done by a first-dimension separation in agarose gel (ph 5.4) followed by a second-dimension separation in horizontal polyacrylamide gel (ph 9.0). this method resulted in improved and reproducible separation of many alpha-globulins. two groups of alpha 1-globulins, designated pi1 and pi2, were found to be protease inhibitors. preliminary studies indicated that pi1 and pi2 proteins differed from each other in molecular weight and ... | 1979 | 94978 |
| [enzymes catalyzing the reduction of aldopentoses and the oxidation of pentitols in mycobacteria]. | m. phlei, grown on synthetic sauton medium (with 6% glycerol as carbon source), had nadph- and nadh-aldopentose reductase, as well as nad-pentitol dehydrogenase activities; some of their properties are studied. these activities are not present in bcg grown on the same medium. all experiments of aldopentose-reductase induction in bcg on a d(+)xylose medium were negative. | 1979 | 120778 |
| regulatory proteins of lobster striated muscle. | the regulatory proteins of lobster muscles consist of tropomyosin and of troponin. troponin contains a 17,000 chain weight component, two closely related components of about 30,000 and a 52,000 chain weight component. in addition to troponin, tropomyosin is required for the inhibition of the magnesium activated actomyosin atpase activity in the absence of calcium and for the reversal of this inhibition by calcium. lobster tropomyosin interacts with rabbit actin and lobster troponin interacts wit ... | 1975 | 123757 |
| [phagocytosis of group a haemolytic streptococci by mouse macrophages. comparison of preliminary infection of animals and degree of virulence of the strain (author's transl)]. | with the aim of studying immunological reactions associated with experimental streptococcal diseases, we have developed an in vitro technique for following phagocytosis of streptococci by peritoneal macrophages of mice. by this means we have been able to show that the rate of phagocytosis of streptococci by "normal" macrophages is inversely correlated to the degree of virulence of the strain. on the other hand, the rate of phagocytosis of the virulent strain is increased 10 times when the macrop ... | 1975 | 126542 |
| membrane transport during development in animals. | this brief and necessarily incomplete survey of available evidence on the development of transport systems in animal cells reveals a primitive state of knowledge full of interesting possibilities for future development. the assembly of membrane-bound transport systems during embryonic development provides unique opportunities for approaching questions relating to gene expression, the synthesis and insertion of membrane proteins into phospholipid layers, the composition and structure of transport ... | 1975 | 126702 |
| [effect of tuberculinization on the blood proteins of camels and cows]. | | 1975 | 129936 |
| [problems of occupational paedagogy in rehabilitation (author's transl)]. | within the framework of a work science focusing on the disabled, occupational paedagogy (arbeitspädagogik) offers- from its methods and objectives- useful approaches to the industrial resettlement of the disabled. the different models of occupational paedagogy and the experience gained from then (taylor, münsterberg, moede, baumgarten, twi-methode, riedel) can be easily adapted to and integrated with special requirements of vocational rehabilitation. in the light of human work valuation as an ex ... | 1976 | 134434 |
| experimental study of biosoluble drugs. thrombus lysis with biosoluble immobilized fibrinolysin in experiment. | | 1978 | 150065 |
| plasma concentration of local anaesthetic agents in regard to absorption, distribution and elimination, with special reference to bupivacaine. | absorption from the injection site, tissue distribution, metabolism and urinary excretion are the factors determining the plasma concentration of local anaesthetic agents. other factors such as physico-chemical properties of these drugs may have important influences on these processes. an example of this is the difference in tissue distribution and plasma/erythrocyte ratio as well as an increase in plasma concentration of bupivacaine seen in acidosis. the importance for the plasma concentration, ... | 1975 | 238555 |
| [efficacy of various culture media in the preservation of yersinia pestis strains]. | | 1979 | 262317 |
| carcinogenicity of antitumor cis-platinum(ii) coordination complexes in the mouse and rat. | | 1979 | 427778 |
| [viral hepatitis following administration of the 4 coagulation-factors concentrate ppsb]. | | 1979 | 492370 |
| insulin responsiveness of adipose tissue from normal weight subjects with early diabetes. | in normal weight subjects, classified by a 2-h glucose infusion test as having normal (11), borderline (3) or pathological (9) carbohydrate tolerance (cht), subcutaneous adipose tissue was removed under intracutaneous anesthesia by surgical biopsy. the biological responsiveness of isolated adipocytes as well as adipose tissue fragments measured as incorportion of (1-14c) glucose into co2 or triglycerides was studied in the absence or presence of different insulin concentrations. in persons with ... | 1979 | 525210 |
| [study of cellular lipids in candida tropicalis k-41 on media with 1-alkenes]. | | 1975 | 1214630 |
| influence of muramyl dipeptide on renal candidiasis in genetically distinct mice. | susceptible (dba/2) and resistant (c57bl/6) mice were inoculated intravenously with candida albicans to evaluate the effect of a four-day prophylaxis with muramyl dipeptide (mdp) on the renal burden of organisms during the first week after infection. in sham-treated dba/2 mice injected with 8 x 10(4) candida cells, renal cfu (log10 +/- sem) on days 1, 4 and 7 after infection were found to average 5.050 +/- 0.109, 4.882 +/- 0.133 and 5.482 +/- 0.245. in sham-treated c57bl/6 mice injected with 2 x ... | 1992 | 1472365 |
| reduction of infarct volume and mortality by thrombolysis in a rat embolic stroke model. | thrombolytic therapy with recombinant tissue plasminogen activator was tested in a rat embolic stroke model. | 1992 | 1636193 |
| cyclic amp-elevating agents block chemoattractant activation of diradylglycerol generation by inhibiting phospholipase d activation. | agents which elevate cellular camp (prostaglandin e2, theophylline, and forskolin) or mimic camp action (dibutyryl camp) are known to inhibit human neutrophil activation (superoxide generation and secretion) by receptor-linked agonists such as formyl-methionyl-leucyl-phenylalanine (fmlp). herein, we show that these agents also markedly inhibit fmlp-stimulated diradylglycerol generation (assayed by mass methods). the magnitude of inhibition correlated with the ability of a given agent or combinat ... | 1991 | 1847376 |
| perinatal development of hepatic cholesterol synthesis in the rat. | rates of cholesterol synthesis and hmg coa reductase activity in rat liver, have been reported to be high before and low after birth. the timing of the decline in perinatal rates of cholesterol synthesis, however, is uncertain. these studies, therefore, determined in vivo rates of cholesterol synthesis using [3h]water and hepatic reductase activity in vitro in perinatal rats. the lipid composition of the plasma, liver and its microsomal subfraction were also determined. reductase activity increa ... | 1991 | 1892876 |
| role of magnesium in the dietary cation-anion balance equation for ruminants. | eight midlactation holstein cows (four primiparous) were assigned to replicated 4 x 4 latin squares with a 2 x 2 factorial arrangement of treatments. the basal diet was formulated to contain 0 milliequivalents (na + k) - cl/100 g of diet dm. treatment diets of high and low dietary cation-anion balance were achieved by adding appropriate amounts of supplemental na + k (added on an equivalent basis) or mg to the basal diet. milk and protein yield were increased as well as dmi and fcm for na + k di ... | 1991 | 1894796 |
| oligonucleotide-directed mutagenesis of psbb, the gene encoding cp47, employing a deletion mutant strain of the cyanobacterium synechocystis sp. pcc 6803. | a mutant strain of the cyanobacterium synechocystis sp. pcc (pasteur culture collection) 6803 has been developed in which psbb, the gene coding for the chlorophyl alpha-binding protein cp47 in photosystem ii (psii), has been deleted. this deletion mutant can be used for the reintroduction of modified psbb into the cyanobacterium. to study the role of a large hydrophilic region in cp47, presumably located on the lumenal side of the thylakoid membrane between the fifth and sixth membrane-spanning ... | 1991 | 1932693 |
| asymptomatic patients with hiv infection. keeping them well. | primary care physicians need to be prepared to counsel and manage patients with human immunodeficiency virus (hiv) infection. asymptomatic seropositive patients should be seen quarterly, and t4 lymphocyte counts should be followed. other serologic markers that may detect disease progression are p24 antigen and beta 2 microglobulin. abnormalities in the levels of these markers may influence the decision to initiate early antiretroviral therapy. therapeutic regimens are now available for delaying ... | 1990 | 1971717 |
| hypoglycemia risk during exercise after intramuscular injection of insulin in thigh in iddm. | the influence of bicycle exercise (60% of w170 [working capacity at a pulse rate of 170 beats/min]; 40 min) on the absorption of 125i-labeled fast-acting insulin (10 u; actrapid human insulin) after intramuscular compared with subcutaneous injection in the thigh was studied on 2 consecutive days in 10 insulin-dependent diabetes mellitus (iddm) patients. insulin absorption was measured as disappearance of radioactivity (1st-order elimination rate constants) by continuous external monitoring and a ... | 1990 | 2190773 |
| purification and fractionation of lipopolysaccharide from gram-negative bacteria by hydrophobic interaction chromatography. | by hydrophobic interaction chromatography on octyl-sepharose, lipopolysaccharide (lps) of escherichia coli re mutant and of wild-type smooth-form (s-form) salmonella typhimurium and salmonella abortus equi is fractionated according to increasing amount of fatty acids. thereby a fractionation of s-form lps according to the length of the o-polysaccharide chain also occurs, because with increasing of fatty acids there is a decrease in the mean length of the o-polysaccharide chain from approximately ... | 1990 | 2269290 |
| a sensitive and specific liquid-chromatographic assay for determination of ganciclovir in plasma and urine and its application to pharmacokinetic studies in the rabbit. | a liquid-chromatographic assay for the analysis of ganciclovir in plasma and urine is described. this assay involves the use of acyclovir, an antiviral drug structurally related to ganciclovir, as the internal standard. a two-step sample preparation method is used. after protein is precipitated with acetonitrile and the addition of diethyl ether, ganciclovir and the internal standard are back extracted into a small volume of aqueous ammonium phosphate, taking advantage of their relatively high w ... | 1990 | 2293208 |
| [immunity status of 2 chilean female populations against rubella]. | a total of 1248 females were studied to determine immunity against rubella. a higher socio-economic subgroup (n = 789) and a lower one (459) were defined. inhibition of hemagglutination was used to detect immunity. positive results were obtained in 95% of females and no differences were detected for both groups. 78% of positive titers fell in the dilution range 1:20 to 1:80. | 1990 | 2293267 |
| nucleotide sequence of porcine insulin-like growth factor. 1:5' untranslated region, exons 1 and 2 and mrna. | | 1990 | 2326169 |
| the 5'- and 3'-untranslated regions of ornithine decarboxylase mrna affect the translational efficiency. | we have determined the roles of the 5'- and 3'-untranslated regions (utr) of ornithine decarboxylase (odc) mrna in the post-transcriptional regulation of this enzyme. a series of expression vectors were constructed in which portions of the odc 5' and/or 3' utrs were placed flanking a reporter gene coding sequence, either firefly luciferase or chloramphenicol acetyltransferase, so as to generate a hybrid transcript. translation of these chimeric genes in transient expression assays in wild type a ... | 1990 | 2365700 |
| neurodevelopmental assessment after congenital cytomegalovirus infection. | the neurodevelopmental state of 41 children with congenital cytomegalovirus infection and their controls was assessed at 2 years using the griffiths scale. the scores achieved by children with congenital cytomegalovirus but with no associated neurological abnormality (asymptomatic) were similar to those of the control children, whereas the mean score of the five children with congenital infection and neurological impairment (symptomatic) was significantly lower. this study, which has the statist ... | 1986 | 2423040 |
| a role for exon sequences and splice-site proximity in splice-site selection. | analysis of the in vitro splicing products of rna precursors containing tandem duplications of the 5' or 3' splice sites of human beta-globin ivs 1 revealed that exon sequences play an important role in the relative use of the duplicated sites. these studies also show that the proximity of the 5' and 3' splice sites is an important determinant in the selection of splice-sites. deletion, substitution, or even subtle changes of exon sequences can alter the pattern of splice-site selection, and in ... | 1986 | 2427200 |
| subtype-specific increase in g-protein alpha-subunit mrna by interleukin 1 beta. | the guanine nucleotide regulatory proteins (g-proteins) which are substrates for adp-ribosylation by pertussis toxin (alpha i-1, alpha i-2, alpha i-3 and alpha o) transduce a variety of hormonal signals. endothelial cells express mrna for three alpha i subtypes although the level of alpha i-1 mrna is very low. interleukin 1 beta (il 1 beta), a pleiotropic inflammatory mediator which stimulates a complex series of responses in human endothelial cells leading to increased coagulation and platelet ... | 1989 | 2525485 |
| co2 laser laparoscopic surgery. adhesiolysis, salpingostomy, laser uterine nerve ablation and tubal pregnancy. | used endoscopically, the co2 laser offers some advantages over other operative techniques for endometriosis and adhesions but, in spite of the continuing development of new instrumentation there are still problems with the system. the technique needs specialized equipment requiring ongoing biomedical maintenance and specialized technical care in the operating room. some problems such as the intraperitoneal accumulation of smoke, gas leakage, and difficulty with maintenance of proper beam alignme ... | 1989 | 2533009 |
| adenylate kinase of mycobacterium marinum. amino acid composition, secondary structure and other aspects. | 1. adenylate kinase purified from mycobacterium marinum shares homology with the enzyme from mammalian muscle and from other bacteria with respect to amino acid composition, total residues and mr. 2. however, it differs from others in not containing a free sulfhydryl or disulfide group. 3. it is much more sensitive to inhibition by adenosine (5')pentaphospho(5')adenosine than is the enzyme from other sources. 4. the enzyme contains 24% alpha-helix, 30% beta-structure and 46% random coil. | 1989 | 2551753 |
| replicative and cytopathic characteristics of hiv-2 and severity of infection. | | 1989 | 2564945 |
| adherence kinetics of haemophilus influenzae type b to eucaryotic cells. | haemophilus influenzae type b (hib) adhere in vitro to buccal epithelial cells (bec) via pili (fimbriae). in vivo studies have conflicting lines of evidence concerning the role of pili in adherence. this study characterizes the kinetics of hib binding to bec and compares pilus versus nonpilus adherence. adherence was assessed using radioactive, immunofluorescence, and electron microscopic techniques. paired hib strains were obtained from three children with hib meningitis; piliated hib were isol ... | 1989 | 2573033 |
| adherence kinetics of haemophilus influenzae type b to eucaryotic cells. | haemophilus influenzae type b (hib) adhere in vitro to buccal epithelial cells (bec) via pili (fimbriae). in vivo studies have conflicting lines of evidence concerning the role of pili in adherence. this study characterizes the kinetics of hib binding to bec and compares pilus versus nonpilus adherence. adherence was assessed using radioactive, immunofluorescence, and electron microscopic techniques. paired hib strains were obtained from three children with hib meningitis; piliated hib were isol ... | 1989 | 2573033 |
| detection of minority point mutations by modified pcr technique: a new approach for a sensitive diagnosis of tumor-progression markers. | the detection of point mutations correlated with diseases, in enzymatically amplified dna sequences (polymerase chain reaction), is currently performed by digestion of pcr products when an existing restriction site disappears at least in one allele of the amplified mutated sequence or by allele specific radiolabeled probes in all other cases. these methods are the most sensitive but they cannot detect a mutation if it is present in less than 5% of the studied cells. we describe here a method bas ... | 1989 | 2573037 |
| [intrathecal perfusion of acnu neurotoxicity and intrathecal pharmacokinetics in dogs]. | we studied the feasibility of intrathecal acnu perfusion therapy against subarachnoid dissemination of malignant glioma. intrathecal perfusion was performed in adult dogs by constant drip administration of 1 to 2 mg acnu dissolved in 10 to 20 ml of lactate ringer solution into the lateral ventricle and cerebrospinal fluid drainage through the lumbar puncture. the perfusion time was changed from 15 to 71 min. a bolus injection of 2 mg acnu was also tested in one dog. no neurological symptom was n ... | 1989 | 2586715 |
| protein kinase c regulates the tonic but not the phasic component of contraction in guinea-pig ileum. | 1. we have investigated the effect of phorbol esters and the down-regulation of protein kinase c on contraction of guinea-pig ileum longitudinal smooth muscle to carbachol and high k+. 2. phorbol 12,13-dibutyrate (pdbu) enhanced the phasic component and inhibited or enhanced, respectively, the tonic component of contraction to carbachol and high k+. in contrast, 4 alpha-phorbol, which does not activate protein kinase c, had no effect on these responses. 3. exposure to phorbol 12-myristate 13-ace ... | 1989 | 2590770 |
| [coagulation and fibrinolysis associated with contact phase in plasma with high molecular weight kininogen deficiency]. | congenital deficiency of high molecular weight kininogen (hmwk), first reported by saito et al. in 1975, is a rare disorder with homozygous defect of hmwk and only 14 cases have been reported so far. prolonged partial thromboplastin time and depressed intrinsic fibrinolytic activity in plasma interacted with negatively charged surface were characterized in these patients without any bleeding problem. on the other hand, patients associated with hemorrhagic disorder, such as haemophiliacs, showed ... | 1989 | 2593236 |
| serum igg level against streptococcus mutans in subjects with caries experience. | serum igg level against streptococcus mutans serotype c was studied in 33 subjects, in relation to dental caries, oral hygiene and age. the activity of the serum igg against the sonicated antigens of the bacterium was evaluated by micro-elisa. the serum igg titer to s. mutans was significantly higher in the subjects with no detectable carious lesions than in the subjects with active caries (p less than 0.05). however, there was no clear difference between the serum igg antibody to s. mutans and ... | 1989 | 2632131 |
| serum igg level against streptococcus mutans in subjects with caries experience. | serum igg level against streptococcus mutans serotype c was studied in 33 subjects, in relation to dental caries, oral hygiene and age. the activity of the serum igg against the sonicated antigens of the bacterium was evaluated by micro-elisa. the serum igg titer to s. mutans was significantly higher in the subjects with no detectable carious lesions than in the subjects with active caries (p less than 0.05). however, there was no clear difference between the serum igg antibody to s. mutans and ... | 1989 | 2632131 |
| [participation of the olfactory system in the regulation of food intake]. | evoked potentials in the olfactory bulb (ob), lateral hypothalamus (hl) and rostral portion of the nucleus of the tractus solitarius (nts), were recorded after cervical vagus nerve stimulation. the slow component in the ob only was recorded in the periglomerular layer. electrolytic lesion of the nts, abolished the evoked potentials in the ob by vagus nerve stimulation. the results of the present experiments indicate that the pathway from the vagus nerve to ob go into the nts but probably not int ... | 1989 | 2633947 |
| impaired in-vitro proliferation of hemopoietic precursors in hiv-1-infected subjects. | patients with acquired immunodeficiency syndrome (aids) and persistent lymphadenopathy syndrome (las) display significant hematological abnormalities of one or more cell lineages. in order to understand the pathophysiologic mechanisms leading to these abnormalities we studied the proliferation capacity of pluripotent and committed hemopoietic precursors using in-vitro colony assays. anemia, leukopenia and thrombopenia were relatively frequent findings in hiv-infected subjects irrespectively of t ... | 1989 | 2668646 |
| impaired in-vitro proliferation of hemopoietic precursors in hiv-1-infected subjects. | patients with acquired immunodeficiency syndrome (aids) and persistent lymphadenopathy syndrome (las) display significant hematological abnormalities of one or more cell lineages. in order to understand the pathophysiologic mechanisms leading to these abnormalities we studied the proliferation capacity of pluripotent and committed hemopoietic precursors using in-vitro colony assays. anemia, leukopenia and thrombopenia were relatively frequent findings in hiv-infected subjects irrespectively of t ... | 1989 | 2668646 |
| isolation of a high frequency donor of rhodobacter capsulatus by integration of the plasmid pmt1000 into the chromosome. | the temperature-independent clone of rhodobacter capsulatus ua7041, carrying the temperature-sensitive plasmid pmt1000, has been obtained by selection for plasmid markers at the non-permissive temperature. the transfer to escherichia coli of all drug resistance encoded by pmt1000 was of about 10(-7) when the donor was the ua7041 strain, and of 5 x 10(-4) when the donor was the temperature-sensitive strain of r. capsulatus. electrophoretic analyses showed no plasmid band typical of the autonomous ... | 1989 | 2679770 |
| [synthetic cephalosporins. v. synthesis and antibacterial activity of 3-alkylthio-7 beta-[(z)-2-(2-aminothiazol-4-yl)-2-(o-substituted oxyimino)acetamido]cephalosporins and related compounds]. | 3-alkylthio-7 beta-[(z)-2-(2-aminothiazol-4-yl)-2-(o-substituted oxyimino)acetamido]cephalosporins (6 and 7) and the 3-methoxy analogues (10) were prepared by coupling diphenylmethyl 7-amino-3-alkylthio-3-cephem-4-carboxylate (1 and 2) or diphenylmethyl 7-amino-3-methoxy-3-cephem-4-carboxylate with (z)-2-(2-tritylaminothiazol-4-yl)-2-(o-substituted oxyimino)acetic acid (4), followed by deprotection and subjected to examination of antibacterial activities. the pivaloyloxymethyl esters (8 and 9) o ... | 1989 | 2698417 |
| effect of a hypertonic lactated ringer's solution on intracranial pressure and cerebral water content in a model of traumatic brain injury. | there has recently been an increased interest in the use of hypertonic solutions for fluid resuscitation of trauma victims. in this study, we examined the acute cerebral effects of a hypertonic lactated ringer's solution (measured osmolality = 469 mosm/kg) in an animal model of traumatic brain injury. following the production of a cerebral cryogenic lesion, eight new zealand white rabbits were randomized to undergo hemodilution with either lactated ringer's (measured osmolality = 254 mosm/kg) or ... | 1989 | 2709456 |
| relationship of serum ferritin and iron concentrations and serum total iron-binding capacity to nonheme iron stores in dogs. | the relationships of various iron-related analytes were evaluated in 95 dogs. liver and spleen nonheme iron content was determined coulometrically on acid-digested tissue specimens. serum iron concentration and total iron-binding capacity also were measured coulometrically, whereas serum ferritin concentration was measured by elisa. significant (p less than 0.0002) correlation was found between serum ferritin concentration and nonheme iron stores. significant correlation was not found between no ... | 1989 | 2719382 |
| altman tapped to be new hcfa administrator. | | 1989 | 2731914 |
| afferent renal nerve effects on plasma vasopressin and oxytocin in conscious rats. | experiments were done in conscious, unrestrained, baroreceptor-intact rats to investigate the effects of afferent renal nerve (arn) stimulation on circulating levels of arginine vasopressin (avp) and oxytocin (oxy). electrical stimulation of arn elicited a rise in arterial pressure (ap) of 12 +/- 2 mmhg and no significant change in heart rate (hr). plasma concentrations of avp and oxy measured by radioimmunoassay were significantly increased immediately after the stimulation period. avp increase ... | 1989 | 2735450 |
| intraerythrocytic ca2+ and mg2+ concentrations of theileria sergenti infected calves. | | 1989 | 2739229 |
| two- and three-dimensional ultrastructure of endothelium and pericyte interdigitations in capillary of human granulation tissue. | two- and three-dimensional electron microscopic observation in the immature capillary of the human granulation tissue revealed cytoplasmic interdigitations (cid) between the endothelium and the pericyte. these were composed of a cytoplasmic projection and indentation, and there was a gap space without basement membrane-like components between the two cell membranes. plasmalemmal vesicles were frequently found locating beneath and/or attached to the cell membranes at the indentated side of the in ... | 1989 | 2769144 |
| decreased 3 alpha-androstanediol glucuronide levels in plasma and random urines in male pseudohermaphroditism caused by 5 alpha-reductase deficiency. | concentrations of 3 alpha-diol glucuronide (3 alpha-diol g) in plasma and/or random urine samples were determined in seven subjects with familial male pseudohermaphroditism (fmp) due to 5 alpha-reductase deficiency (5 alpha-rd). all subjects were natives of an isolated turkish village with a high incidence of consanguineous marriage. a specific and sensitive antibody to 3 alpha-diol was used for radioimmunoassay of 3 alpha-diol g after hydrolysis and chromatographic purification. the mean plasma ... | 1989 | 2770532 |
| post-irradiation regeneration of early b-lymphocyte precursor cells in mouse bone marrow. | to examine the sequential development of early b-cell precursors in mouse bone marrow, b-lineage cells have been examined during a wave of post-irradiation regeneration. cell phenotypes have been defined using double-immunofluorescence labelling techniques for (i) terminal deoxynucleotidyl transferase (tdt); (ii) b220 glycoprotein, detected by the binding of mab 14.8; (iii); mu heavy chains in the cytoplasm (c mu) and at the cell surface (s mu). three populations of mu- cells (tdt+14.8-; tdt+14. ... | 1989 | 2784779 |
| signet ring cell lymphoma of t cell type. | a rare variant of non-hodgkin's lymphoma, signet ring lymphoma of t cell phenotype (only the fourth to be reported) in a 75 year old man was studied by light microscopy, immunohistochemistry, electron microscopy and gene rearrangement studies. ultrastructurally, a wider spectrum of cell size and nuclear shape was observed in this case than in the previously recorded cases. the morphology of the signet ring vacuoles was identical to that found in the commoner b cell signet ring lymphoma of clear ... | 1989 | 2784803 |
| [spontaneous interleukin 1 secretion by alveolar macrophages from the bronchoalveolar lavage of patients with active sarcoidosis]. | alveolar macrophages from 28 patients with pulmonary sarcoidosis, from 6 patients with idiopathic pulmonary fibrosis and from 8 control subjects were tested for their spontaneous interleukin-1 release. whereas alveolar macrophages from the controls and from the patients with idiopathic pulmonary fibrosis did not show a significant release of interleukin 1, 14 of the sarcoidosis patients displayed a considerable spontaneous interleukin-1 secretion. besides radiographic signs of an increased disea ... | 1989 | 2789181 |
| [spontaneous interleukin 1 secretion by alveolar macrophages from the bronchoalveolar lavage of patients with active sarcoidosis]. | alveolar macrophages from 28 patients with pulmonary sarcoidosis, from 6 patients with idiopathic pulmonary fibrosis and from 8 control subjects were tested for their spontaneous interleukin-1 release. whereas alveolar macrophages from the controls and from the patients with idiopathic pulmonary fibrosis did not show a significant release of interleukin 1, 14 of the sarcoidosis patients displayed a considerable spontaneous interleukin-1 secretion. besides radiographic signs of an increased disea ... | 1989 | 2789181 |
| comparative antimicrobial efficacy of soft and rigid gas permeable contact lens solutions against acanthamoeba. | ten soft contact lens cleaners, three rigid gas permeable contact lens (rgp) disinfecting-soaking solutions, and four hydrophilic contact lens disinfecting solutions were evaluated by laboratory challenge procedures for their antimicrobial efficacy against trophozoites and cysts of acanthamoeba polyphaga and acanthamoeba castellanii. miraflow was the only cleaner that killed trophozoites and cysts on all lenses during the cleaning step. a disinfecting solution preserved with chlorhexidine and on ... | 1989 | 2805312 |
| comparative antimicrobial efficacy of soft and rigid gas permeable contact lens solutions against acanthamoeba. | ten soft contact lens cleaners, three rigid gas permeable contact lens (rgp) disinfecting-soaking solutions, and four hydrophilic contact lens disinfecting solutions were evaluated by laboratory challenge procedures for their antimicrobial efficacy against trophozoites and cysts of acanthamoeba polyphaga and acanthamoeba castellanii. miraflow was the only cleaner that killed trophozoites and cysts on all lenses during the cleaning step. a disinfecting solution preserved with chlorhexidine and on ... | 1989 | 2805312 |
| unusual dna sequences located within the promoter region and the first intron of the chicken pro-alpha 1(i) collagen gene. | genomic clones corresponding to the amino-terminal propeptide and 5'-flanking sequences of the chicken pro-alpha 1(i) collagen gene were isolated as a first step in the identification of dna sequences important for transcriptional regulation of the pro-alpha 1(i) collagen gene. due to the failure to identify positive clones in either primary or amplified genomic libraries, a 5.1-kilobase pair stui genomic fragment identified by southern blotting was enriched by sucrose gradient fractionation of ... | 1987 | 2820966 |
| onset of steroidogenesis and differentiation of functional lh receptors in rat fetal testicular cultures. | the present study was performed to investigate in vitro the onset of steroidogenesis and the responsiveness to lh in rat fetal testes. the male gonads explanted on days 12.5, 13.5, and 14.5 of gestation in m199 produced testosterone from 15.5 days as is the case in vivo dcamp (1 mm) induced an anticipated steroidogenesis on day 14.5 with secretions of testosterone (0.026 +/- 0.003 ng/gonad/24 h) and progesterone (0.078 +/- 0.005 ng/gonad/24 h), whereas lh (100 ng/ml) has no effect. antiandrogens ... | 1987 | 2823733 |
| shift in genomic molecular patterns of human rotaviruses over an eighteen-month period in hungary. | rotavirus electropherotypes of 103 fecal samples collected from children for 18 months in baranya county, hungary, revealed seven different patterns. two electropherotypes were predominant and a clear shift was evident in their occurrence. the results provide the first evidence of a shift in the prevalence of electropherotyping variants in hungary. | 1987 | 2832346 |
| delayed response deficit in monkeys by locally disturbed prefrontal neuronal activity by bicuculline. | effects of local injection of a gamma-aminobutyric acid antagonist, bicuculline (10-30 micrograms dissolved in saline) in the principal prefrontal cortex on the delayed response task were investigated in two monkeys. on a visual go signal, the monkeys rotated a handle to the left or to the right (go period) according to a visual cue (left or right; 1 s) presented 4 s earlier. bicuculline induced bursting activity at the injected site 1-2 min after the injection and 5 min later the burst activity ... | 1988 | 2849457 |
| a murine monoclonal antibody raised against human non-small cell carcinoma of the lung. | murine monoclonal antibodies (mabs) reactive with human non-small cell carcinoma of the lung (nsccl) were produced following immunization with a membrane preparation of calu-3, a human adenocarcinoma of the lung cell line grown in nude mice. positive hybrids unreactive with normal liver membranes and human peripheral white blood cells were selected for further testing. one mab (rs5-4h6) recognized an antigen expressed in a variety of lung and other carcinoma cell lines as detected by flow cytome ... | 1988 | 2853133 |
| naloxone attenuation of sham feeding is modified by manipulation of sucrose concentration. | the time course of sucrose (5, 10 and 20%; w/v) sham feeding was monitored in one hour tests. intake levels increased as a function of concentration. naloxone (1.25 mg/kg, ip) attenuated the sham feeding of 10% sucrose solution in gastric fistulated rats, without affecting initial intake rates. furthermore, after naloxone the intake pattern of 10% sucrose was identical to that for 5% sucrose in untreated rats. in a second test, substitution of 10% sucrose by a 20% solution after 15 min of sham f ... | 1988 | 2853384 |
| bayesian diagnosis in presence of pre-existing disease. | clinicians are increasingly facing complex diagnostic problems in patients who have a pre-existing disease and in whom new symptoms develop which may be complications of that disease or manifestations of another, perhaps more common, condition. the neurological complications of malignancy fall into this situation. the results of diagnostic tests applied under these circumstances run the risk of being misinterpreted, largely due to lack of appreciation of the powerful effect of the pre-existing d ... | 1985 | 2857373 |
| a new glucocorticoid receptor detected in host rat liver but not in various hepatomas. | previously a new glucocorticoid receptor (peak c), which eluted with 0.12 to 0.14 m nacl from deae-cellulose column, was identified in addition to another receptor (peak b), a classic type of glucocorticoid receptor, which eluted with 0.05 to 0.08 m nacl. peak c appeared after stress or injection of a high dose (20 micrograms/100 g body weight) of dexamethasone into rats. peak c was also detected in the liver of rats bearing various tumors, but it was not found in malignant tumors (yoshida sarco ... | 1987 | 2885083 |
| immunocytochemical studies on thyroid parafollicular cells in postnatal development of the rat. | studies were performed on wistar strain rats aged 1-720 days. immunocytochemical reactions were used to detect calcitonin, somatostatin, calcitonin gene-related peptide (cgrp), cholecystokinin, serotonin, neuron-specific enolase (nse), secretory protein-i, chromogranin and ca-binding protein. in the parafollicular cells of the rat, the presence of calcitonin, somatostatin, cgrp, nse and secretory protein-i could be demonstrated. the number of parafollicular cells increased with the age of animal ... | 1987 | 2893508 |
| neonatal thyroid disease: differential expression in three successive offspring. | neonatal thyroid disease, manifested as either hyperthyroidism or hypothyroidism, has been described in the offspring of women with autoimmune thyroid disorders. we report clinical and laboratory observations in a mother with autoimmune thyroiditis who gave birth to three children with different forms of thyroid dysfunction. the first child was clinically normal, the second child had transient neonatal hyperthyroidism, and the third child had neonatal hypothyroidism. after the birth of the secon ... | 1988 | 2895120 |
| synthesis of proteins in escherichia coli immunoreactive with sera from individuals infected with human t-cell leukemia virus type i. | the env-px iv fused gene of human t-cell leukemia virus type i (htlv-i) was inserted into lac promoter-directed expression vectors for production of viral proteins in bacteria. resulting recombinant plasmids, pk13 and pk15, directed synthesis of fused proteins of 59 kda (env-p40x) and 100 kda (gag-env-p40x), respectively. western blot analysis showed that these proteins were reactive with sera of patients with adult t-cell leukemia (atl) and retained multiple antigenic determinants of viral prot ... | 1988 | 2898099 |
| synthesis of proteins in escherichia coli immunoreactive with sera from individuals infected with human t-cell leukemia virus type i. | the env-px iv fused gene of human t-cell leukemia virus type i (htlv-i) was inserted into lac promoter-directed expression vectors for production of viral proteins in bacteria. resulting recombinant plasmids, pk13 and pk15, directed synthesis of fused proteins of 59 kda (env-p40x) and 100 kda (gag-env-p40x), respectively. western blot analysis showed that these proteins were reactive with sera of patients with adult t-cell leukemia (atl) and retained multiple antigenic determinants of viral prot ... | 1988 | 2898099 |
| mitochondrial atp synthase: role of metal binding in structure and function. | | 1988 | 2901755 |
| [results and prospects for the study of endemic goiter in kazakhstan]. | the results of many-year-old research into endemic goiter epidemiology in kazakhstan were summed up. methods for the assessment of potential goiter risk were worked out. multipurpose expedition investigations including mass screenings of the population and animals in all climatic and geographic zones of the republic, an analysis of the concentration of iodine and some other trace elements in the environment and the level of their consumption by man, resulted in compiling a variety of medicogeogr ... | 1987 | 2950511 |
| [interferon production in vitro]. | interferon production in vitro was induced by poly i: c and pha in leukocytes obtained from patients with cancer, benign diseases and control subjects. the interferon response per lymphocyte was relatively constant in all groups. however, interferon production was slightly inhibited in patients receiving cancer chemotherapy. the peak response of interferon occurred at 48 hours after the initiation of the combined culture in each specimen. from our observations, it was suggested that interferon p ... | 1987 | 2952067 |
| human milk-derived lactoferrin inhibits mitogen and alloantigen induced human lymphocyte proliferation. | purified lactoferrin (lf), isolated from human milk, was tested for its effect on human t-lymphocyte proliferative responses to phytohemagglutinin (pha) and to alloantigens in mixed lymphocyte culture. lf inhibited proliferation in both assays in a dose-dependent manner. the suppressive effect was not due to lf mediated cytotoxicity since washing cells that had been pre-incubated with lf restored their proliferative activity. lf was most effective in suppressing the pha response when added withi ... | 1987 | 2963123 |
| stringhalt in horses: a distal axonopathy. | a detailed investigation of the neuropathology of a horse affected with stringhalt was performed. qualitative and quantitative light and electron microscopy, and single teased fibre preparations of peripheral nerve demonstrated predominantly axonal degeneration, the stage of which was appropriate for the duration of clinical signs. there was selective involvement of large myelinated nerve fibres. a proximal to distal increase in the severity of pathological changes was present in the peripheral ... | 1986 | 3024050 |
| intranuclear vacuoles in creutzfeldt-jakob disease. | in an electron microscopic study of brain biopsy material from a case with creutzfeldt-jakob disease intranuclear vacuoles containing membrano-vesicular structures were found. to our knowledge this finding has not been previously reported in human spongiform encephalopathies. it may represent a specific alteration associated with creutzfeldt-jakob disease, and suggests the possibility of nuclear infection. | 1986 | 3024051 |
| simian immunodeficiency virus induces expression of class ii major histocompatibility complex structures on infected target cells in vitro. | the human immunodeficiency virus (hiv) and the closely related simian immunodeficiency virus (siv) induce profound immune dysfunction in primate species. the present studies show that cell populations infected in vitro with siv exhibit increases in major histocompatibility complex (mhc) class ii antigen expression. cell lines chronically infected with both the monkey and human viruses express substantially more mhc class ii but not more lineage-restricted or activation antigens on their membrane ... | 1987 | 3033270 |
| utilization of glutamate accumulating bacterial cells for production of ribonucleotides. | corynebacterium glutamicum cells are industrially used for glutamate production. however, the waste that contains microbial cells, cellular debris, residual sugars, ammonia and metabolites seriously pollutes the environment. the cells are recovered and utilized for ribonucleotide production so that the pollution caused by the cells is eliminated. nucleic acid is extracted from the cells and is hydrolyzed with nuclease p1 from penicillium citrinum. the hydrolysate is fractionated with dowex-50 an ... | 1985 | 3033725 |
| isolation of a cdna clone for the dihydrolipoamide acetyltransferase component of the human liver pyruvate dehydrogenase complex. | dihydrolipoamide acetyltransferase (e2) forms the structural core of pyruvate dehydrogenase complex. a cdna clone (lambda e2-1) for mammalian e2 was identified from a human liver lambda gt11 library using anti-e2 serum. affinity-selected antibodies using the fusion protein from lambda e2-1 immuno-reacted specifically with e2 of purified pyruvate dehydrogenase complex on immuno-blot analysis. the cdna insert was approximately 2.3 kb in length with an internal ecor1 site generating 1.4 and 0.9 kb ... | 1987 | 3036145 |
| partial purification and characterization of camp-dependent protein kinase from fasciola hepatica. | three cyclic amp (camp)-dependent protein kinases, designated a1, a2, and b, were isolated from the liver fluke fasciola hepatica using phenyl-sepharose and deae-cellulose chromatography. these enzymes differed with respect to activation by camp and their molecular weights. the half-maximal activation constant for camp-dependent protein kinases a1 and b was 20 nm, while that of a2 was about five-fold higher (110 nm). the estimated molecular weights for camp-dependent protein kinases a1 and a2 (b ... | 1987 | 3037368 |
| malpais spring virus: a new vesiculovirus from mosquitoes collected in new mexico and evidence of infected indigenous and exotic ungulates. | two virus isolates, 1 each from aedes campestris and psorophora signipennis mosquitoes collected in south central new mexico in august 1985, were shown by neutralization tests to be identical to each other, but not to any of more than 250 arthropod-borne and other viruses. electron microscopy of 1 isolate (85-488nm, chosen as the prototype) indicated that this strain shares morphologic characteristics with viruses of the family rhabdoviridae. indirect fluorescent antibody tests indicated that th ... | 1988 | 3061310 |
| histochemical identification of guinea-pig macrophages by monoclonal antibody mr-1. | a monoclonal antibody is described which reacts with monocytes and macrophages of the guinea-pig. the antibody recognizes a cytoplasmic component in the majority of tissue macrophages, as detected by histochemical analysis. it reacts positively with all macrophages in lymphoid organs, including tingible body macrophages. in addition it recognizes the cells of von kupffer in the liver, and macrophages in bone marrow and peritoneal cavity. in comparison to other monoclonal antibodies that have bee ... | 1988 | 3065217 |
| acrosomal proteinase activity of human spermatozoa and relation of results to semen quality. | an assay for measuring the acrosomal proteinase activity of human spermatozoa with the use of a microscope slide coated with a thin layer of gelatin as a protein substrate was developed and applied to study the semen of 82 males from infertile couples and 15 normal donors. acrosomal protease activity is indicated by a clear area of proteolyzed gelatin (halo) about the sperm head. probit analysis revealed two populations divided at a halo diameter of 14 microns. the patient samples were grouped a ... | 1988 | 3068243 |
| the contribution of cyclooxygenase and lipoxygenase products to acute inflammation in the rat. | | 1986 | 3083659 |
| some properties of lipoxygenase activities in cytosol and microsomal fractions of mouse epidermal homogenate. | lipoxygenase activity in microsomal fraction of mouse epidermal homogenate was characterized in comparison with cytosol lipoxygenase activity. the major activity was identified as 12-lipoxygenase in microsomal fraction as well as in cytosol fraction by the analyses with high-performance liquid chromatography and gas chromatography-mass spectrometry. apparent km values of cytosol and microsomal 12-lipoxygenase for arachidonic acid were 5.0 microm and 6.2 microm respectively. apparent vmax values ... | 1986 | 3085110 |
| some properties of lipoxygenase activities in cytosol and microsomal fractions of mouse epidermal homogenate. | lipoxygenase activity in microsomal fraction of mouse epidermal homogenate was characterized in comparison with cytosol lipoxygenase activity. the major activity was identified as 12-lipoxygenase in microsomal fraction as well as in cytosol fraction by the analyses with high-performance liquid chromatography and gas chromatography-mass spectrometry. apparent km values of cytosol and microsomal 12-lipoxygenase for arachidonic acid were 5.0 microm and 6.2 microm respectively. apparent vmax values ... | 1986 | 3085110 |
| attempts to modify changes in the piglet small intestine after weaning. | the possible causes of changes in the piglet small intestine after weaning were investigated. groups of piglets weaned at three weeks old were either given oral oxytetracycline daily, weaned on to a regularly fed sow-milk replacer or weaned on to specially formulated diets based on casein or hydrolysed casein as the protein source. after five days, changes in small intestinal structure and brush-border enzyme activities were compared with previous findings in weaned and unweaned animals of the s ... | 1986 | 3090663 |
| attempts to modify changes in the piglet small intestine after weaning. | the possible causes of changes in the piglet small intestine after weaning were investigated. groups of piglets weaned at three weeks old were either given oral oxytetracycline daily, weaned on to a regularly fed sow-milk replacer or weaned on to specially formulated diets based on casein or hydrolysed casein as the protein source. after five days, changes in small intestinal structure and brush-border enzyme activities were compared with previous findings in weaned and unweaned animals of the s ... | 1986 | 3090663 |
| the ability of single sex infections of schistosoma japonicum to induce resistance to reinfection in mice. | in four experiments, mice harbouring an average 50, 76 or over 200 schistosoma japonicum female worms were not resistant when challenged six to eight weeks after infection. the female worms from these single sex infections were stunted and immature (average length 4.6 mm) and induced no overt pathology in the host. male worm burdens of 60, 135 or 140 also induced little or no resistance to challenge in the host. the males from these single sex infections were fully grown for their age (average l ... | 1986 | 3091678 |
| aortic endothelial and smooth muscle cell interactions in culture. | the effect of conditioned media on cell growth was studied in pig aortic smooth muscle cell conditioned medium on pig or bovine aortic endothelial cells and pig or bovine endothelial cells conditioned medium on pig aortic smooth muscle cell proliferation. co-culturing experiments were also done in the same combinations. cell proliferation was measured in both systems by 3h-thymidine incorporation autoradiography. in intraspecies combinations the media conditioned by confluent pig-aortic endothel ... | 1986 | 3111182 |