Publications

effects of poly(1-vinyluracil) and poly(9-vinyladenine) on viral rna-directed dna polymerase.	the effects of poly(1-vinyluracil) [poly(vu)] and poly(9-vinyladenine) [poly(va)] on the rna-dependent dna polymerase activity of murine leukemia virus (moloney strain) were studied. vinyl polymers themselves cannot act as templates for the polymerase. however, if a vinyl polymer is added to a polymerase reaction mixture in which a complementary polynucleotide serves as the template, the reaction is inhibited: thus with polyribocytidylic acid as template and oligodeoxyguanylic acid as primer, ne ...	1976	56995
overview. the major histocompatibility system of the rat.		1979	92081
evaluation of pseudorabies viral antigens in the agar gel immunodiffusion test.	procedures designed to extract pseudorabies viral (prv) antigens from prv-infected tissue cultures were investigated to determine whether differences in extraction method had an effect upon the final concentrated antigenic product. all four of the preparations made from prv-infected tissue culture cells (trypsin extract and disrupted cells) or entire prv-infected cultures (polysorbate 80 extract and (nh4)2so4 precipitate) contained relatively large amounts of the same antigen, whereas cell-free ...	1979	92905
effect of colicins ia and e1 on ion permeability of liposomes.	colicins ia and e1 are shown to inhibit the formation and bring about the collapse of a potassium diffusion potential imposed across the membrane of liposomes prepared from soybean or escherichia coli phospholipids. such depolarization results from a colicin-induced increase in membrane ion permeability. colicins e2 and e3 do not depolarize such membranes. in addition to the colicin ia-induced rapid efflux of preloaded rubidium, sodium, phosphate, or choline from liposomes, a slower efflux of pr ...	1979	93288
comparison of antigen-specific i-region-associated cell interaction factors.	two basic types of factors reacting with anti-i region (anti-ia) antisera are compared, those derived from macrophage-like antigen presenting cells and others derived from t-lymphocytes, of either the suppressor or helper type. despite the common property of reacting with anti-ia antisera, the two sets of factors differ by many criteria. macrophages, upon culture with antigen, release complexes of ia antigen and a fragment of the original immunogen. this material is only produced by responder ma ...	1979	93870
hereditary alpha 2-macroglobulin deficiency.	on screening of a normal material 1 man was found (age 37) who on repeated determinations had a low alpha 2m, namely 25 % with electroimmuno assay according to laurell. investigation of the family revealed that the mother (age 69) and one daughter (age 5) had low values too. all other coagulation and fibrinolytic components were normal. they had no signs of increased fibrinolysis and normal levels of alpha 2-antiplasmin, alpha 1-antitrypsin and antithrombin iii. liver function tests were normal. ...	1979	94459
the role of antigen-specific t cell factors in the immune response.		1979	94504
genetic polymorphism and close linkage of two alpha 1-protease inhibitors in horse serum.	two-dimensional electrophoretic analysis of horse serum proteins was done by a first-dimension separation in agarose gel (ph 5.4) followed by a second-dimension separation in horizontal polyacrylamide gel (ph 9.0). this method resulted in improved and reproducible separation of many alpha-globulins. two groups of alpha 1-globulins, designated pi1 and pi2, were found to be protease inhibitors. preliminary studies indicated that pi1 and pi2 proteins differed from each other in molecular weight and ...	1979	94978
treatment of severe herpes keratitis with cryosurgery and immunotherapy.	the contrasting type of lesion produced in the first eye of non-immune and the second eye of the then immune rabbits using herpes simplex type i virus is demonstrated. cryosurgery may make the first eye worse but the second eye is healed. a large percentage of second eyes heal spontaneously, however, as circulating antibody titres are high. artefacts may be mistaken for lesions in the immune eye but superficial viral growth has a characteristic plaque-like appearance. one hundred eyes with recur ...	1979	95060
production and chemical studies of protease from bacillus subtilis (sh-6) egyptian strain.	bacillus subtilis (sh-6) egyptian strain, isolated from hides, gave the highest protease activity. luxurious growth and protease production were obtained by the use of a medium containing 8% of potato starch, 0,1 m of ammonium phosphate as carbon and nitrogen sources. results indicate that borate buffer exerted a deleterious effect on the protease production. comparing citrate and phosphate buffers, it was found that citrate gave lower protease activity than phosphate. there is a positive respon ...	1978	98932
congeners of dom: effect of distribution of the evaluation of pharmacologic data.		1978	101880
antibodies against measles antigen in the laboratory housed rhesus and bonnet monkeys.		1979	107117
mineralization of the enamel of ovine permanent central incisor teeth using microhardness and histological techniques.		1979	116728
[hypothalamic hypophysiotropic hormones: biochemical and clinical aspects. i. thyroliberin and luliberin].		1979	117441
effect of actinomyces viscosus on the establishment and symbiosis of streptococcus mutans and streptococcus sanguis in spf rats on different sucrose diets.		1975	123171
the role of the pituitary in acne.		1976	138539
effect of human immunoglobulin preparations on fc rosette formation between anti-d-coated erythrocytes and lymphocytes.	human immunoglobulin (ig) preparations were tested for their inhibitory effect on fc rosette formation between anti-d-coated human erythrocytes and lymphocytes, as compared to their complement activating capacity. both of the two biological activities ascribed to the sites in the fc portion of the igg molecules were found to be reduced in the pepsin-treated, as well as in the s-sulfonated ig preparations, as compared to the activities of the normal human ig preparation. in the plasmin-treated ig ...	1978	150144
studies on the composition of pig serum lipoproteins. isolation and characterization of different apoproteins.	1. different lipoprotein density fractions from pig serum were isolated by phosphotungstate precipitation followed by purification in the preparative ultra-centrifuge. 2. the protein part of very low density lipoproteins was composed of approximately 52 percent lipoprotein b apoprotein and the rest of lipoprotein c ii apoprotein and other as yet unidentified peptides. 3. the protein moiety of low density lipoproteins consisted primarily of lipoprotein b apoprotein (over 95 percent); the amino ac ...	1975	166686
a circular dna-protein complex adenoviruses and its possible role in dna replication.		1975	169089
characterization of sv40 dna rescued from transformed mouse cells.		1975	169101
in vitro translation of encephalomyocarditis viral rna: synthesis of capsid precursor-like polypeptides.		1976	178097
electron microscopical and enzyme histochemical changes in the rat myocardium during prolonged autolysis.	the effect on rat myocardium of autolysis at 19 degrees c, for up to 20 days, was studied by electron microscopy and enzyme histochemistry. the enzymes studied included monoamine oxidase (mao), sytochrome oxidase (cyto), non-specific esterase ((ns.e.), phosphorylase (p-ase), succinate dehydrogenase (sdh) and various nad- and nadp-linked dehydrogenases. the myocardium lost its histochemical p-ase activity within a few hours of autolysis, whereas the activity of all other enzymes remained quite no ...	1976	179522
the storage lipids in tangier disease. a physical chemical study.	the physical states and phase behavior of the lipids of the spleen, liver, and splenic artery from a 38-yr-old man with tangier disease were studied. many intracellular lipid droplets in the smectic liquid crystalline state were identified by polarizing microscopy in macrophages in both the spleen and liver, but not in the splenic artery. the droplets within individual cells melted sharply over a narrow temperature range, indicating a uniform lipid composition of the droplets of each cell. howev ...	1977	193870
particle-bound enzymes in the bloodstream form of trypanosoma brucei.	we have screened the bloodstream form of trypanosoma brucei for the presence of enzymes that could serve as markers for the microbodies and the highly repressed mitochondrion of this organism. none of seven known microbody enzymes were detected at all, but glycerol-3-phosphate oxidase, atpase, isocitrate dehydrogenase, acid phosphatase and part of the hyperoxide dismutase and malate dehydrogenase activities were found to be particle-bound after fractionation of homogenates by differential centri ...	1977	195809
mutagenicity of diallate, sulfallate, and triallate and relationship between structure and mutagenic effects of carbamates used widely in agriculture.	in an investigation of the mutagenic properties of 20 carbamate herbicides and fungicides by use of the salmonella/microsome mutagenicity test as developed by ames et al. (mutation res., 31: 347-364, 1975), we have found that three thiocarbamate compounds, diallate, sulfallate and triallate, are mutagenic in the presence of a liver microsomal fraction on strains ta1535 and ta100. this indicates that the metabolic products of these thiocarbamates are causing base-pair substitutions. since the 2-c ...	1978	201368
isolation and partial characterization of rat cns axolemma enriched fractions.	axolemma-enriched fractions were prepared from rat brain by osmotic shock of a purified preparation of myelinated axons and subsequent separation of myelin, two axolemma-enriched fractions and myelin-free axons by density gradient centrifugation. compared with the starting whole homogenate, the fractions were enriched in specific activity of na+k+ atpase, acetylcholinesterase, 5'nucleotidase as well as 2',3'-cyclic nucleotide 3'phosphohydrolase. compared with myelin, the axolemmal fractions are ...	1978	206316
effects of ascorbate loading on pmn bacterial iodination and other functions.		1978	211233
skin papillomas in an impala (aepyceros melampus) and a giraffe (giraffa camelopardalis).	viral particles, typical of the papovavirus family, were demonstrated by electronmicroscopy in small papillomas found on the feet of an impala (aepyceros melampus) and on the face of a giraffe (giraffa camelopardalis) in kenya. histologically the tissues proved to be typical papillomas. the viral particles measured 38 nm and 40 nm in diameter in all tissue sections from the impala and giraffe respectively.	1978	211271
comparison of interferon action in interferon resistant and sensitive l1210 cells.	translation inhibition, leu-trna aminoacylation and double-stranded rna and atp dependent phosphorylation were examined in interferon-treated and control cell-free lysates of leukaemic mouse l 1210 r and l 1210 s cells. no differences were observed between the respective interferon-treated and control cell-free extracts, except for the presence of an enhanced 67k dalton phosphoprotein fraction in interferon-treated l 1210 s cell-free extracts. in non-responding cell-free lysates, the lack of sti ...	1978	212514
interactions of gonadotropins with corpus luteum membranes. i. properties and distributions of some marker enzyme activities after subcellular fractionation of the superovulated rat ovary.	the properties of a number of enzyme activities of the superovulated rat ovary have been studied to establish optimal assay conditions and specific assay procedures for each activity. the activities were chosen on the basis of their extensive use in other tissues of the rat as marker enzymes for the major cell organelles. homogenates of superovulated rat ovaries were subjected to fractionation by differential rate centrifugation, and sedimentation profiles were constructed for each marker enzyme ...	1978	217656
mucin-producing atypical bronchial carcinoid.	in a case of atypical mucin-producing bronchial carcinoid in a 40-year-old man covert metastases were present at the time of lobectomy and were revealed at necropsy--a feature highlighting the malignant potential of the atypical carcinoid.	1978	219040
immune responses during human schistosomiasis mansoni. i. in vitro lymphocyte blastogenic responses to heterogeneous antigenic preparations from schistosome eggs, worms and cercariae.	in vitro lymphocyte blastogenesis assays were performed using peripheral blood lymphocytes obtained from patients with schistosomiasis mansoni. their infection was well characterized both clinically and in regard to the duration and intensity of schistosoma mansoni infection. subjects who were either not infected with any helminths or who were negative for s. mansoni but infected with other helminths provided two control groups. cultures were exposed to various concentrations of heterogeneous so ...	1977	300716
pregastric esterase and other oral lipases--a review.	the secretion of pregastric esterase and other oral lipases has been detected in 13 species. research on secretion by the human, calf, kid goat, lamb, and rat of pregastric esterase has been significant. secretion by calves is little affected by age or diet but is greater when calves are nipple fed than when pail fed. whole milk sham-fed to calves exhibits immediate, sharp decreases in ph and rennet coagulation time resulting from liberation of free fatty acids by pregastric esterase. bacterial ...	1977	321489
eosinophil chemotactic factor (ecf). iii. generation in human peripheral leukocytes.	eosinophil chemotactic factor (ecf) can be released from human peripheral leukocytes by an ige-anti-ige reaction, by the calcium ionophore a 23187 and during phagocytosis. supernatants and sonicates of unstimulated cells contain little or no ecf. on stimulation, however, ecf activity increases in the cells and even more so in the supernatants. this holds for purified neutrophils (pmns) as well as for basophil-containing mononuclear cell preparations. these findings contrast with those in lung ho ...	1978	344230
potency of 10 bcg vaccines as evaluated by their influence on the bacillemic phase of experimental airborne tuberculosis in guinea-pigs.		1979	387796
the cachexia of cancer.	this review examines the contributions made by anorexia, loss of taste, malabsorption and disturbances of intermediary metabolism to the cachexia of cancer. methods of nutritional assessment are outlined and mention is made of the usefulness of nutritional support as an adjunct to anti-cancer therapies.	1979	393313
[the molecular biology of aging -- 15: structure and function of the genetic apparatus in aging. ii. synthesis and metabolism of rna].	with aging in different organs there are similar or tissue specific changes of the ribonucleic acids in respect of the quantity, the spectrum of different rna species and the metabolism of rna. among these processes the template function of the chromatin and the behaviour of the rna polymerases are important influencing factors. in aging the sensitivity of the rna synthesis against hormonal stimulation is decreased in the most cases. a survey about the results of many in vivo and in vitro experi ...	1979	395770
activation of tumoricidal properties in mouse macrophages by lymphokines encapsulated in liposomes.	cell-free culture supernatants rich in macrophage-activating factor (maf) activity obtained from mitogen-stimulated f344 rat lymphocytes have been encapsulated within liposomes of differing size and lipid composition and their ability to render normal mouse macrophages cytotoxic for tumor cells in vitro has been compared with that of unencapsulated (free) maf added to the extracellular medium. normal macrophages from c57bl/6, c3h/hen, and c57bl/6 x c3h f1 mice treated with liposome-encapsulated ...	1979	427777
a microtechnique for the rapid analysis of macromolecule synthesis in minicultures of mammalian cells.	a new micromethod, called the stanzen technique, is described for the rapid determination of dna and protein content as well as the incorporation rates of radioactively labeled precursors into macromolecules in cells growing in replica minicultures on plastic petri dishes. the procedure yielded reproducible results assaying only minimal cell numbers per sample and was applied for studying both primary or early passaged cell cultures (mouse epidermal cells and fibroblasts) and a malignantly trans ...	1979	478561
neurectoderm markers retained in phenotypical skeletal muscle cells arising from a glial cell line.		1979	492321
[toxicologic-hygienic characteristics of the preparation nerosin, an agent for protecting plants and soils subject to wind erosion].	a year's experiment on albino rats fed with bread prepared from spring wheat "saratovskaya-29" grown with the utilization of the antideflationary agent nerosin (1.5 t/hectare) showed that this bread did not effect the functional condition of the liver (the antitozic and protein-forming functions), as well as the redox activity of the blood constituents catalase and peroxidase, and the morphological structure of the peripheral blood of the animals. an elevation of glucuronic acid metabolism (33.1 ...	1979	516602
experimental s. mansoni infection in mice. blood white cells, serum proteins, glycemia and blood urea in various stages of the infection.		1979	545638
[hypothetical concept: the physiopathological entity of monoaminergic psychoses].	the importance of amino acid transport across the blood-brain barrier as the limiting factor in the metabolism of monoamines has been emphasized by many recent publications. particularly critical is the transport of tryptophan, since tryptophan hydroxylase is not saturated. this transport is regulated by complex mechanisms, both at the periphery (total and free plasmatic levels and levels of the other essential amino acids) and centraly (by feedback mechanism initiated at the pre- and post-synap ...	1975	1082812
endogenous prostaglandin release contributes directly to coronary artery tone.	an in vitro coronary artery preparation of beef heart was found to synthesize and release continuously large amounts of a prostaglandin of the e type. inhibition of prostaglandin synthesis with aspirin, indomethacin, or eicosa-5,8,11,14-tetraynoic acid induced a sustained contraction of the coronary artery, and pretreatment with indomethacin diminished markedly the output of prostaglandin into the bathing medium. it appears that prostaglandin e1, generated from within the vessel wall itself, may ...	1975	1175084
contrast and electrolyte dynamics of the intravenous pyelogram. ii. disparities in serum and urine concentrations of meglumine and anion.	intravenous or intra-aortic injections of meglumine iothalamate in dogs indicate a slightly different tissue distribution and excretory pattern of the meglumine cation and the iothalamate anion. differences in serum and urinary concentrations of these ions suggest some cellular penetration of the meglumine anion. in addition, there seems to be some evidence for both tubular absorption and tubular excretion of meglumine as a minor component in the renal excretion of this ion. no significant diffe ...	1975	1184320
[primary structure of cytoplasmic aspartate aminotransferase from pig heart muscle. isolation, purification and characterization of thermolytic peptides].		1975	1203378
actions of parathyroid hormone-related protein as an embryonic differentiation factor.		1992	1300340
[effects of hypokinesia on cardiac activity in rats with high and low spontaneous motor activity].	hypokinesia effects on cardiac contractivity and vegetative regulation were studied in rats with high and low spontaneous motor activity. it was shown that hypokinesia promotes depression of the stroke volume and decrease of sympathetic nervous activity in rats with low motor activity. at the same time, in rats with high motor activity these parameters were not significantly affected by hypokinesia. the conclusion is made about a relationship between resistance to hypokinesia and spontaneous mot ...	1992	1301100
linkage of atypical myotonia congenita to a sodium channel locus.	we performed linkage analysis in a pedigree segregating an allele for autosomal dominant, painful myotonia that is potassium sensitive and responsive to acetazolamide. this allele was tightly linked to a skeletal-muscle, sodium channel locus which is now a candidate for the site of the mutational defect in acetazolamide-responsive myotonia congenita. since this sodium channel locus is completely linked to the disease allele in all hyperkalemic periodic paralysis and paramyotonia congenita pedigr ...	1992	1310531
permeability and charge-dependent adsorption properties of the s-layer lattice from bacillus coagulans e38-66.	we investigated the permeability properties of the oblique s-layer lattice from bacillus coagulans e38-66 after depositing cell wall fragments on a microfiltration membrane, cross-linking the s-layer protein with glutaraldehyde, and degrading the peptidoglycan with lysozyme. comparative permeability studies on such multilayered s-layer membranes and suspended s-layer vesicles from thermophilic members of the family bacillaceae with use of the space technique (m. sára and u. b. sleytr, j. bacteri ...	1992	1317378
freeze-dried, cross-linked bovine type i collagen: analysis of properties.	this study was undertaken to assess the physical and biological properties of freeze-dried cross-linked bovine type i collagen and to assess its potential for use in the guided tissue regeneration method of treatment of periodontal disease in human adult subjects. the modulus of elasticity, swelling ratio, and biodegradation rate were investigated. the collagen sponge was implanted subdermally into sprague-dawley rats and a histological study carried out at 2, 7, 21, 35, and 49 days post implant ...	1992	1317425
stimulation of rat pancreatic tumoral ar4-2j cell proliferation by pituitary adenylate cyclase-activating peptide.	in the present work the effects of the novel neuropeptide pituitary adenylate cyclase activating peptide (pacap) on both ar4-2j cell growth and the modulation of ornithine decarboxylase activity were investigated. both pacap38 and the amidated form pacap27 caused a concentration-dependent stimulation of ar4-2j cell growth; the maximal increase was seen at 1 nmol/l (30% above control, p less than 0.01) with a half-maximal effect at 0.01 nmol/l. ornithine decarboxylase activity was also increased ...	1992	1323494
structure of holo-chaperonin studied with electron microscopy. oligomeric cpn10 on top of two layers of cpn60 rings with two stripes each.	a structural model of holo-chaperonin, known as a protein-folding control protein comprising 60 kda (cpn60) and 10 kda polypeptides (cpn10), is proposed based on the electron microscopic images of holo-chaperonin from thermus thermophilus and cpn60 from paracoccus denitrificans. isolated paracoccus cpn60 shows very similar images to those of escherichia coli tetradecameric cpn60, a seven-membered ring in the top view and a rectangular shape with four stripes in the side view. however, a small nu ...	1992	1347504
characterization of guanosine diphospho-d-mannose dehydrogenase from pseudomonas aeruginosa. structural analysis by limited proteolysis.	alginate is believed to be a major virulence factor in the pathogenicity of pseudomonas aeruginosa in the lungs of patients suffering from cystic fibrosis. guanosine diphospho-d-mannose dehydrogenase (gdpmannose dehydrogenase, ec 1.1.1.132) is a key enzyme in the alginate biosynthetic pathway which catalyzes the oxidation of guanosine diphospho-d-mannose (gdp-d-mannose) to gdp-d-mannuronic acid. in this paper, we report the structural analysis of gmd by limited proteolysis using three different ...	1992	1370473
thallium-201 accumulation by epidermoid inclusion cyst.	an elderly male undergoing re-evaluation for coronary artery disease demonstrated an extra-cardiac focus of 201tl accumulation during the performance of a planar myocardial perfusion scan. this corresponded to a subcutaneous lesion of the left posterior thorax found on a concurrent computerized axial tomographic (cat) scan and upon surgical excision proved to be an "epidermoid inclusion cyst." a follow-up nuclear scan failed to reveal any residual extracardiac tracer localization. confusing this ...	1992	1403158
antibody responses to natural dictyocaulus viviparus exposure in first year grazing cattle using vaccination or different kinds of anthelmintic treatment.	in two field trials vaccination or different anthelmintic treatments against dictyocaulus viviparus infections were used in 6 groups of first year grazing cattle. the antibody response to lungworm infections was determined using an elisa. cattle treated once or repeatedly at long intervals with levamisole developed clinical signs of dictyocaulosis. the detection of anti-d. viviparus antibodies at the end of the grazing season confirmed that these anthelmintic treatments were not able to prevent ...	1992	1462721
on 'the natural history of hiv and aids in women'.		1992	1472349
an evaluation of the effect of the anesthetic agent profofol (diprivan) on the outcome of human in vitro fertilization.		1992	1482846
reversal by concanavalin a of the inhibitory effects of extracellular ca2+ on pinocytosis in amoeba proteus.	when concanavalin a, 1-20 micrograms ml-1 binds to the surface of amoeba proteus the cell's response to ca2+ and to cationic inducers of pinocytosis is strikingly altered. separately, concanavalin a and ca2+ are weak inducers but their combined effects are intense pinocytosis and suppression of the normal inhibitory effect of ca2+ on cation-induced pinocytosis. at high concentrations (greater than 25 micrograms ml-1) the lectin increases cellular uptake and binding of 45ca in the cell surface bu ...	1992	1595356
cd4 envelope interactions of hiv-1 and related human retroviruses.		1991	1781342
[proline specific peptidases and their specific inhibitors].	several proline specific peptidases were newly isolated from mammalian organs, plants and microorganisms, and their enzymatic properties characterized. the genes of prolyl endopeptidase, aminopeptidase p and proline iminopeptidase from some microorganisms were cloned and their nucleotide sequences determined. by high expression of these genes in escherichia coli, the enzymes became possible to be used for the industrial application as well as its basic research. novel inhibitors specific for the ...	1991	1783984
growth arrest induced by transforming growth factor beta 1 is accompanied by protein phosphatase activation in human keratinocytes.	protein phosphorylation and dephosphorylation are involved in regulation of cell growth. we tested the hypothesis that the growth inhibitory effect of transforming growth factor beta 1 (tgf-beta 1) involves activation of protein phosphatases. exposure of human keratinocytes in culture to 400 pm tgf-beta 1 for 48 h led to 80% inhibition of dna synthesis as measured by nuclear labeling. incubation of cultured keratinocytes with 400 pm tgf-beta 1 rapidly activated (within 30 min) protein serine/thr ...	1991	1847373
miniature manipulator with microelectrode for chronic recording of single units from freely-moving animals.	we report a simple recording and stimulating system, a miniature manipulator with a microelectrode (mmm) that can be mounted on animal's skull. the system consists of a miniature manipulator and a microelectrode which are used in one body. several mmms can be implanted simultaneously into the brain as the part of the manipulator is small. with this device, recording of single units from several different cortices is possible from awake and unrestricted animals.	1991	1851979
plasminogen polymorphism in libyans: description of a new rare variant.	the genetic polymorphism of human plasminogen (plg) was investigated in libyans using wide-scale ultrathin-layer polyacrylamide isoelectric focusing with subsequent immunoblotting. the 2 common alleles, plga and plgb, and 4 previously reported rare ones, plga3, plgm4, plgb1 and plgb2, were observed. in addition, a new intermediate rare allele designated plgmtripoli (plgmt) was encountered. the estimated allele frequencies for the genes plga, plgb, plga3, plgmt, plgm4, plgb1 and plg ...	1991	1855784
preserved co2 response in cerebral and muscular blood vessels during cimetidine treatment.	it is known that cimetidine inhibits the hypoxia-induced increase in cerebral blood flow (cfb) in dogs, but the mechanism of this inhibition is not fully understood. since the accepted mechanisms explaining the increase in cbf during hypercapnia are very different from those active during hypoxia, acute hypercapnia was induced in 12 conscious dogs in order to study the cimetidine effect in this condition. six dogs were given i.v. saline (control group) and the other six, i.v. cimetidine (4 mg kg ...	1991	1914439
control of enterococcus faecalis sex pheromone cad1 elaboration: effects of culture aeration and pad1 plasmid-encoded determinants.	aeration of plasmid-free enterococcus faecalis strains resulted in an 8- to 16-fold decrease in sex pheromone cad1 activity in culture filtrates. levels of two unrelated pheromones, cpd1 and cam373, were unaffected by culture aeration. aeration also resulted in a decrease in the expression of conjugative transfer functions observed in cells containing pad1 trab mutations, verifying a link between trab function and pheromone "shutdown." tests with a series of pad1 mini-plasmids indicated that the ...	1991	1924555
[carcinogenic effects of low-dose gamma ray irradiation].	for a dose of 3 gy delivered by cobalt 60 gamma rays on rats, a reduction of the dose rate by a factor of 60 decreased the carcinogenic effectiveness by a factor of about 5. this decreased effect was essentially observed for carcinomas.	1990	2129111
cloning of human dopamine receptor.		1990	2144333
preparation and characterization of anti-paroxetine antibodies.	6-nitroparoxetine was synthesized and reduced to 6-aminoparoxetine. after coupling to glutaraldehyde at the 6-position and to bovine serum albumin, the resulting schiff's base was further reduced into an amino-derivative which served as the antigen. anti-paroxetine antibodies were raised against this antigen in rabbits and the anti-paroxetine igg purified by protein a affinity chromatography. the anti-paroxetine igg demonstrated high specificity towards paroxetine and 6-nitroparoxetine without s ...	1990	2147049
[glass ionomer cements as orthodontic bracket adhesives. an in vitro study with 4 glass ionomer cements (gic) and 2 conventional orthodontic bracket adhesives as the comparative group].	240 orthodontic brackets were bonded to buccal surfaces of bovine teeth. as bonding material we used four glass ionomer cements without etching and two normal orthodontic bracket bonding materials with etching of enamel. brackets of group 1 were tested with a material testing machine for shear and tensile strength after ten minutes. brackets of group 2, 3 and 4 were tested after one day, 28 days and 98 days, respectively. in the average the bonding strength of glass ionomer cements was 50% less ...	1990	2227735
[glass ionomer cements as orthodontic bracket adhesives. an in vitro study with 4 glass ionomer cements (gic) and 2 conventional orthodontic bracket adhesives as the comparative group].	240 orthodontic brackets were bonded to buccal surfaces of bovine teeth. as bonding material we used four glass ionomer cements without etching and two normal orthodontic bracket bonding materials with etching of enamel. brackets of group 1 were tested with a material testing machine for shear and tensile strength after ten minutes. brackets of group 2, 3 and 4 were tested after one day, 28 days and 98 days, respectively. in the average the bonding strength of glass ionomer cements was 50% less ...	1990	2227735
glucocorticoid stimulation of fatty acid synthesis in explants of human fetal lung.	we examined the effects of glucocorticoids and thyroid hormone (t3) on fatty acid synthesis, fatty acid composition and fatty acid synthetase activity in explants of human fetal lung (16-23 wk gestation). explants were cultured 1-7 days in the absence (control) or presence of dexamethasone (10 nm) and/or t3 (2 nm). in control explants fatty acid synthesis and fatty acid synthetase activity increased 200% and 455%, respectively, between 1 and 5 days. dexamethasone (10 nm) stimulated fatty acid sy ...	1990	2297514
nucleotide sequence of porcine insulin-like growth factor. 1:5' untranslated region, exons 1 and 2 and mrna.		1990	2326169
autonomic regulation of the pulmonary circulation.	responses to vagal stimulation, acetylcholine, catecholamines, and sympathetic nerve stimulation were investigated in the feline pulmonary vascular bed under conditions of controlled pulmonary blood flow and constant left atrial pressure. results of these studies suggest that, in the cat, the vagus is composed of efferent fibers from both the sympathetic and parasympathetic systems. the data also suggest that the feline pulmonary vascular bed is functionally innervated by cholinergic nerves, and ...	1985	2409403
antibodies to distal carboxyl terminal epitopes in the v-fms-coded glycoprotein do not cross-react with the c-fms gene product.	the product of the v-fms oncogene is an integral transmembrane glycoprotein that is closely related to the cell surface receptor for the macrophage colony stimulating factor, csf-1. a fragment of the v-fms gene encoding a major portion of the extracellular amino terminal domain, the membrane-spanning segment, and the entire carboxyl terminal tyrosine kinase domain of the glycoprotein was molecularly cloned into an inducible prokaryotic expression plasmid. polypeptide products consisting only of ...	1986	2425481
the behaviour of sensory ganglia in relation to their environment.	the nodose ganglion was removed from rats and transplanted into the denervated tongue and into the denervated or innervated sternocleidomastoid muscle. in another procedure the ganglion was sutured to the distal hypoglossal or sciatic nerve. the ganglia failed to develop motor end-plates on denervated muscle. they did, however, induce formation of taste buds on the lingual epithelium. the regenerated nerve fibres ramified extensively within the connective tissue, invading perivascular tissue and ...	1989	2606773
histophysiological changes of sex organs in methylmercury intoxicated mice.	methylmercury (mehg) treatment intramuscularly at doses of 10 and 20 micrograms separately/day/mouse for 30 days affected the weight of body and organs like testes, vas deferens and seminal vesicles in mice. diminution of testosterone in serum was also associated with an accumulation of cholesterol in testes and reduction in seminal vesicle fructose. altered spermatogenesis and leydig cell morphology in testes and the changes in histoarchitecture of seminal vesicle and vas deferens indicated and ...	1989	2714228
steroid hormone receptors and the sexual phenotype of the harderian gland in hamsters.	to investigate the participation of intracellular steroid hormone receptors in the sexual transformation process of the harderian gland, a series of experiments were undertaken in adult golden hamsters. the invitro labelling of cytosolic steroid-binding sites with appropriate radioligands revealed the presence of androgen, oestrogen and glucocorticoid but not progestin receptors in the glands from animals of both sexes. the androgen receptor of the female gland was further characterized because ...	1989	2715754
differential effect of hemin-controlled eif-2 alpha kinases from mouse erythroleukemia cells on protein synthesis.	cultured mouse erythroleukemia (mel) cells can be induced to erythroid differentiation by a variety of chemical agents. this differentiation process is marked by the onset of globin mrna and hemoglobin synthesis. in rabbit reticulocytes, globin synthesis is regulated by a hemin-controlled translational inhibitor (hci) which acts via phosphorylation of the alpha subunit of eukaryotic initiation factor 2 (eif-2). from both uninduced and induced mel cells, hemin-controlled eif-2 alpha kinases have ...	1989	2753041
studies on the regenerated carotid sinus nerve of the rabbit.	1. the central end of the distally cut left carotid sinus nerve was sutured to the tunica media of the external carotid artery, 1 cm cranial to the carotid bifurcation, in nineteen rabbits. the carotid body was removed in fourteen of these rabbits but left in situ in the remaining five. after 56-165 days of recovery a neuroma was identified at the site of the suture. ventilatory reflexes mediated by both sinus nerves were tested and afferent activity recorded from the regenerated nerve. 2. venti ...	1989	2795484
the effect of thiamin supplementation on the thiamin status of turkey poults with ethanol-induced cardiomegaly.	1. turkey poults were treated with graded doses (0-5% v/v) of ethanol for 6 weeks. some birds were concomitantly injected intramuscularly with thiamin. 2. erythrocyte transketolase (tk) activity was significantly decreased and the erythrocyte pyrophosphate (tpp) effect and blood concentrations of lactate and pyruvate were statistically greater in all ethanol-treated poults by 42 days. 3. thiamin restored tk activity to normal levels and decreased the tpp effect and blood concentrations of lactat ...	1987	2820834
survival of smallpox virus.		1985	2857360
survey of experimental data on fetal liver transplantation.	fetal liver transplantation has been shown to induce hematological and immunological reconstitution in irradiated rodents, dogs, horses, and sheep. engraftment and reconstitution without gvhd has been readily obtained using histocompatible donors. when mismatched fetal donors were used, a comparatively larger number of donor cells was required, in addition to pre-treatment of host with higher doses of irradiation or irradiation plus chemotherapy. stem cell suspensions devoid of any t lymphocyte ...	1987	2893475
arylamine n-acetyltransferase from chicken liver. i. monoclonal antibodies, immunoaffinity purification, and amino acid sequences.	five monoclonal antibodies against arylamine acetyltransferase (ec 2.3.1.5) from the chicken liver were established by immunizing a mouse with a partially purified enzyme preparation. none of the antibodies cross-reacted with arylamine n-acetyltransferase from the livers of cow, rabbit, and rat, nor with arylalkylamine n-acetyltransferase from the chicken pineal gland, indicating a high specificity of the antibodies. by using the antibodies, two immunoaffinity purification procedures were elabor ...	1988	2897359
in vitro metabolism and covalent binding among ortho-substituted bromobenzenes of varying hepatotoxicity.	a series of ortho-substituted bromobenzene derivatives with widely differing hepatotoxicities were investigated for their tendency to undergo oxidative metabolism and protein covalent binding in the presence of rat liver microsomes in vitro. compounds studied included o-bromobenzonitrile, o-dibromobenzene, bromobenzene, o-bromoanisole, and o-bromotoluene (names in order of decreasing hepatotoxicity and increasing rate of in vitro metabolism). no correlation was found between net covalent binding ...	1988	2898339
[3h]sch 23390 binding to d1 dopamine receptors in the basal ganglia of the cat and primate: delineation of striosomal compartments and pallidal and nigral subdivisions.	the distribution of d1 dopamine receptors was studied autoradiographically in the basal ganglia of the cat, monkey and human. these receptor binding sites were labeled directly with the d1-selective antagonist [3h]sch 23390, and ligand-binding assays were performed concurrently. serial- or same-action analysis permitted comparisons among d1 binding distributions, acetylcholinesterase staining and tyrosine hydroxylase immunoreactivity. in all species studied, the dorsal striatum exhibited patches ...	1988	2901690
synapses from bipolar cells onto dopaminergic amacrine cells in cat and rabbit retinas.	dopaminergic amacrine cells in the vertebrate retina have long been characterized as 'interamacrine' as they were only found to be pre- and postsynaptic to other amacrine cells. immunohistochemistry with antibodies directed against tyrosine hydroxylase (th) revealed synapses from bipolar cell axon terminals to th-containing neuronal processes at ribbon synapses in the rhesus monkey retina. this finding challenged the notion of the dopaminergic amacrine cell phenotype as 'interamacrine'. in order ...	1988	2906268
increased interleukin-1 production and monocyte suppressor cell activity associated with human tuberculosis.	interleukin-1 (il-1) production by blood monocytes and blastogenesis produced by tuberculin purified protein derivative (ppd) in blood t lymphocytes were examined in patients with pulmonary tuberculosis. monocytes were isolated from blood mononuclear cells by plastic adherence, and il-1 activity was determined in the mouse thymocyte proliferation assay. monocytes from patients with tuberculosis produced significantly higher activities of il-1 than did those from healthy tuberculin reactors when ...	1986	2935057
in vitro fluid dynamic characteristics of aortic bioprostheses: old versus new.	the in vitro fluid dynamic characteristics of a variety of old- and new-generation porcine and pericardial aortic bioprostheses were investigated under pulsatile flow conditions. the pressure drop results indicate that the old valve designs have performance indices in the range of 0.30 to 0.42, while the new, low-pressure fixed designs have performance indices of 0.50 to 0.70. leaflet photography indicated that the new designs also have superior opening and closing characteristics. flow visualiz ...	1986	2937982
endocytosis of proteoheparan sulfate by cultured skin fibroblasts.	endocytosis by cultured human skin fibroblasts of 35so4(2-)-labeled proteoheparan sulfate from fibroblast secretions was quantitatively investigated. at a constant dose of proteoheparan sulfate, uptake and subsequent intracellular degradation but not binding to the cell membrane increased with time. compared with the endocytosis of total secreted proteoglycans containing predominantly a small proteodermatan sulfate, uptake of proteoheparan sulfate was 5- to 20-fold lower. nevertheless, uptake of ...	1986	2943303
comparison of human growth hormone levels in children with satisfactory and unsatisfactory growth.	sixty-six children with satisfactory growth and 73 with unsatisfactory growth (both sexes) from three different age groups (1 year, 5 years and 10 years) were included in this study. it was observed that the serum concentrations of human growth hormone (hgh) in children with satisfactory growth ranged from 1.00 to 4.00 ng/ml and never exceeded 4.00 ng/ml. in a comparative study there was a significant increase in serum hgh levels in subjects of all age groups who were not growing satisfactorily. ...	1987	2956220
human milk-derived lactoferrin inhibits mitogen and alloantigen induced human lymphocyte proliferation.	purified lactoferrin (lf), isolated from human milk, was tested for its effect on human t-lymphocyte proliferative responses to phytohemagglutinin (pha) and to alloantigens in mixed lymphocyte culture. lf inhibited proliferation in both assays in a dose-dependent manner. the suppressive effect was not due to lf mediated cytotoxicity since washing cells that had been pre-incubated with lf restored their proliferative activity. lf was most effective in suppressing the pha response when added withi ...	1987	2963123
secretion of anti-müllerian hormone by immature bovine sertoli cells in primary culture, studied by a competition-type radioimmunoassay: lack of modulation by either fsh or testosterone.	secretion of anti-müllerian hormone (amh) by immature bovine sertoli cells in primary culture was studied through a competition-type ria employing a polyclonal antibody and 125i-labelled purified amh. this ria is approximately 10 times more sensitive than the solid-phase two-site monoclonal antibody-based ria described previously. biosynthesis and secretion of amh by cultured sertoli cells require approximately 48 h, are not influenced by fsh or testosterone and steadily decrease over a one-week ...	1985	3000849
the epidemiology of the acquired immunodeficiency syndrome.		1986	3008169
absence of a unique relationship between active transport of lactose and protonmotive force in e. coli.	the relationship between active transport of lactose via the lactose permease and the protonmotive force has been determined in e. coli cells using either the respiratory chain inhibitor cyanide or protonophores to decrease the protonmotive force progressively. in contradiction with the prediction of the delocalized chemiosmotic theory, two different relationships were obtained depending on the method used.	1986	3025020
[determination of salmonella typhimurium antigens in the excreta of children with salmonellosis using the passive hemagglutination reaction].	1,390 samples of different excreta obtained from salmonellosis patients have been tested for the presence of s. typhimurium o- and h-antigens. s. typhimurium antigens, detected with the use of antibody diagnostica, have been found to occur more frequently than s. typhimurium cells. particulate o- and h-antigens capable of agglutinating antibody diagnostica are excreted differently with saliva and urine. salmonella antigens are best detected in feces in the passive hemagglutination test with the ...	1988	3057776
[determination of salmonella typhimurium antigens in the excreta of children with salmonellosis using the passive hemagglutination reaction].	1,390 samples of different excreta obtained from salmonellosis patients have been tested for the presence of s. typhimurium o- and h-antigens. s. typhimurium antigens, detected with the use of antibody diagnostica, have been found to occur more frequently than s. typhimurium cells. particulate o- and h-antigens capable of agglutinating antibody diagnostica are excreted differently with saliva and urine. salmonella antigens are best detected in feces in the passive hemagglutination test with the ...	1988	3057776
the 'antimutagenic' effect of cinnamaldehyde is due to a transient growth inhibition.	addition of cinnamaldehyde to the selective medium causes a reduction in the number of revertant colonies of s. typhimurium or e. coli when the cells have been mutagenized with 4nqo but not when they have been mutagenized with mnng. toxicity of the cinnamaldehyde exposure depends largely on the status of growth and/or nutrient supply of the cells. we present evidence that simple growth inhibition due to lack of nutrients mimics the effect of cinnamaldehyde in 4nqo- and mnng-treated cells. this a ...	1988	3138535
decline of naturally acquired antibodies to hepatitis b surface antigen in hiv-1 infected homosexual men.		1988	3146272
antibody detection of hiv envelope and core proteins.		1988	3146275