| hormonal control of zinc uptake and binding in the rat dorsolateral prostate. | the zinc uptake in the dorsolateral prostate of rats was studied after different hormonal manipulations. orchiectomy reduced the uptake of 65zn. administration of estradiol benzoate to orchiectomized rats doubled the 65zn uptake, a phenomenon which was not observed in orchiectomized-adrenalectomized rats. adrenalectomy in orchiectomized rats had no effect on the concentration of radioactivity beyond the castration-induced decrease. a prolactin release inhibitor, 6-methyl-8-erogelenylacetamide, r ... | 1977 | 15955 |
| [study of the dynamics of acid formation in the stomachs of dogs under the influence of thyroidin by the method of intragastric ph-graphy]. | intragastral ph-graphy was applied to the study of the dynamics of acid production in the stomach of dogs with fistulae of the fundal portion of the stomach out of and during digestion, and also before and after the thyroidin prescription. biphasic character of the changes in acid production in the stomach under the effect of the hormonal preparation was revealed. | 1978 | 31210 |
| oxygen metabolism by the anaerobic bacterium veillonella alcalescens. | | 1978 | 31846 |
| cell-mediated immune response in mice treated with steroidal contraceptives. | the splenomegaly assay (simonsen, 1962) was standardized using different strains of rats and mice. male wistar rat (donor)-female swiss mouse (host) was found to be the suitable combination that could be employed in subsequent experiments to study the potential of contraceptive steroids to alter cmir. the index of splenomegaly appeared to increase in case of mice treated with combination oral contraceptives (ovulen, ovral or enovid). the differences observed, however, neared significance only in ... | 1975 | 55327 |
| effects of poly(1-vinyluracil) and poly(9-vinyladenine) on viral rna-directed dna polymerase. | the effects of poly(1-vinyluracil) [poly(vu)] and poly(9-vinyladenine) [poly(va)] on the rna-dependent dna polymerase activity of murine leukemia virus (moloney strain) were studied. vinyl polymers themselves cannot act as templates for the polymerase. however, if a vinyl polymer is added to a polymerase reaction mixture in which a complementary polynucleotide serves as the template, the reaction is inhibited: thus with polyribocytidylic acid as template and oligodeoxyguanylic acid as primer, ne ... | 1976 | 56995 |
| a serial study of pregnancy proteins in primigravidae. | the plasma concentrations from four 'pregnancy proteins' and three steroid hormones have been measured throughout pregnancy in 15 primigravidae. two of the proteins, human placental lactogen (hpl) and pregnancy-specific beta1-glycoprotein (psbetag), are specific for pregnancy and correlate well with the stage of gestation. it is suggested that measurement of psbetag may be useful in assessing feto-placental wellbeing. neither of the pregnancy-associated proteins, steriod-binding beta-globulin (s ... | 1976 | 57799 |
| procaine-induced seizures in epileptic monkeys with bilateral hippocampal foci. | intravenous procaine hcl given at low doses (0.5-2.5 mg/kg) to two monkeys with bilateral alumina hippocampal foci depressed interictal spiking or had little effect. at 5.0 mg/kg unilateral limbic activation occurred. at 10.0 mg/kg unilateral or bilateral limbic activation and generalized seizures could be evoked within 3-10 min. at higher doses (15 and 20 mg/kg) bilateral limbic activation or brief (one min) generalized seizures occurred. the unilateral-onset psychomotor seizures were not ident ... | 1979 | 91502 |
| evaluation of pseudorabies viral antigens in the agar gel immunodiffusion test. | procedures designed to extract pseudorabies viral (prv) antigens from prv-infected tissue cultures were investigated to determine whether differences in extraction method had an effect upon the final concentrated antigenic product. all four of the preparations made from prv-infected tissue culture cells (trypsin extract and disrupted cells) or entire prv-infected cultures (polysorbate 80 extract and (nh4)2so4 precipitate) contained relatively large amounts of the same antigen, whereas cell-free ... | 1979 | 92905 |
| characterization of various horse thyroid forms of cyclic-nucleotide phosphodiesterase [proceedings]. | | 1979 | 92956 |
| comparison of antigen-specific i-region-associated cell interaction factors. | two basic types of factors reacting with anti-i region (anti-ia) antisera are compared, those derived from macrophage-like antigen presenting cells and others derived from t-lymphocytes, of either the suppressor or helper type. despite the common property of reacting with anti-ia antisera, the two sets of factors differ by many criteria. macrophages, upon culture with antigen, release complexes of ia antigen and a fragment of the original immunogen. this material is only produced by responder ma ... | 1979 | 93870 |
| has myelin basic protein received a fair trial in the treatment of multiple sclerosis? | autosensitization to some central nervous system antigen still remains one of the best hypotheses for the continuing pathogenesis of multiple sclerosis (ms). enough is now known about the cause, pathogenesis, and treatment of experimental allergic encephalomyelitis (eae) to test this hypothesis. reports of therapeutic failure of the encephalitogen myelin basic protein (bp) in the treatment of ms have their counterparts in similar therapeutic failures in eae. only highly inbred strain 13 guinea p ... | 1979 | 93873 |
| hereditary alpha 2-macroglobulin deficiency. | on screening of a normal material 1 man was found (age 37) who on repeated determinations had a low alpha 2m, namely 25 % with electroimmuno assay according to laurell. investigation of the family revealed that the mother (age 69) and one daughter (age 5) had low values too. all other coagulation and fibrinolytic components were normal. they had no signs of increased fibrinolysis and normal levels of alpha 2-antiplasmin, alpha 1-antitrypsin and antithrombin iii. liver function tests were normal. ... | 1979 | 94459 |
| treatment of severe herpes keratitis with cryosurgery and immunotherapy. | the contrasting type of lesion produced in the first eye of non-immune and the second eye of the then immune rabbits using herpes simplex type i virus is demonstrated. cryosurgery may make the first eye worse but the second eye is healed. a large percentage of second eyes heal spontaneously, however, as circulating antibody titres are high. artefacts may be mistaken for lesions in the immune eye but superficial viral growth has a characteristic plaque-like appearance. one hundred eyes with recur ... | 1979 | 95060 |
| productivity of ospreys in connecticut--long island increases as dde residues decline. | nesting success of ospreys (pandion haliaetus) breeding in the connecticut--long island area has increased since 1973 and is now approaching the levels recorded prior to the 1950's. simultaneously, dde and dieldrin residues have declined in unhatched eggs. levels of polychorinated biphenyls have shown no changes over the period 1969 to 1976. the increase in productivity is attributed primarily to lower levels of dde contamination. detrimental effects in the past on ospreys in the connecticut riv ... | 1978 | 99818 |
| effect of actinomyces viscosus on the establishment and symbiosis of streptococcus mutans and streptococcus sanguis in spf rats on different sucrose diets. | | 1975 | 123171 |
| spherule wall formation by physarum polycephalum. | | 1977 | 143675 |
| effect of human immunoglobulin preparations on fc rosette formation between anti-d-coated erythrocytes and lymphocytes. | human immunoglobulin (ig) preparations were tested for their inhibitory effect on fc rosette formation between anti-d-coated human erythrocytes and lymphocytes, as compared to their complement activating capacity. both of the two biological activities ascribed to the sites in the fc portion of the igg molecules were found to be reduced in the pepsin-treated, as well as in the s-sulfonated ig preparations, as compared to the activities of the normal human ig preparation. in the plasmin-treated ig ... | 1978 | 150144 |
| letter: petechiae: due to adenovirus type 7 infection or to acetylsalicylic acid therapy? | | 1975 | 165451 |
| cytosine arabinoside- and interferon-mediated control of polyoma and sv40 genome expression. | by metabolic dna inhibitors such as arac, viral as well as host dna replication is suppressed in polyoma- and sv40-infected cells. the interruption of the current viral dna replication has no effect on the current transcription of the late viral genes. the persistence of the late transcription indicates that the onset, but not the persistence, of the viral dna replication is a prerequisite for the persistence of the late polyoma and sv40 genome transcription. pretreatment of monkey kidney cells ... | 1975 | 169073 |
| antibiotic treatment of respiratory sepsis. | | 1976 | 176923 |
| the effect of hypoxia on collagen synthesis in cultured 3t6 fibroblasts and its relationship to the mode of action of ascorbate. | when exposed to low oxygen tension, in the absence of added ascorbic acid 3t6 mouse fibroblast cultures in late log phase respond by increased lactate production and increased hydroxylation of proline in nascent collagen, which is paralleled by an increase in prolyl hydroxylase activity. after 6 h recovery from the anoxic stimulus, however, cultures still yield more prolyl hydroxylase than controls, but the effect on hydroxylation of nascent collagen has disappeared. these observations help to d ... | 1976 | 183826 |
| regulation of norepinephrine release from cardiac sympathetic fibers in the dog by presynaptic alpha- and beta-receptors. | the effect of phenoxybenzamine (pba), desmethylimipramine (dmi), clonidine (clnd), sotalol (stl), and isoproterenol (ispr) on the release of endogenous norepinephrine (ne) from the heart on right cardioaccelerator nerve stimulation was studied in anesthetized dogs. under control conditions, the catecholamine levels in coronary sinus blood increased linearly with increasing frequencies of stimulation up to 10 hz and did not increase further at 30 hz. the release of ne was markedly enhanced after ... | 1977 | 193655 |
| evidence for hydroxyl radical generation by human monocytes. | a number of highly reactive oxygen species have been implicated in the oxygen-dependent mechanisms involved in bactericidal activity of phagocytic leukocytes. hydrogen peroxide and superoxide, two agents known to occur during phagocytosis, are thought to interact to generate hydroxyl radical, singlet oxygen, and other potentially reactive molecules. using an assay system of ethylene generation from methional, cell preparations of human monocytes were demonstrated to generate hydroxyl radical or ... | 1977 | 194926 |
| comparison of interferon action in interferon resistant and sensitive l1210 cells. | translation inhibition, leu-trna aminoacylation and double-stranded rna and atp dependent phosphorylation were examined in interferon-treated and control cell-free lysates of leukaemic mouse l 1210 r and l 1210 s cells. no differences were observed between the respective interferon-treated and control cell-free extracts, except for the presence of an enhanced 67k dalton phosphoprotein fraction in interferon-treated l 1210 s cell-free extracts. in non-responding cell-free lysates, the lack of sti ... | 1978 | 212514 |
| pregastric esterase and other oral lipases--a review. | the secretion of pregastric esterase and other oral lipases has been detected in 13 species. research on secretion by the human, calf, kid goat, lamb, and rat of pregastric esterase has been significant. secretion by calves is little affected by age or diet but is greater when calves are nipple fed than when pail fed. whole milk sham-fed to calves exhibits immediate, sharp decreases in ph and rennet coagulation time resulting from liberation of free fatty acids by pregastric esterase. bacterial ... | 1977 | 321489 |
| synthesis and properties of fluorescent nucleotide substrates for dna-dependent rna polymerases. | a new class of fluorescent nucleotide analogs which contain the fluorophore 1-aminonaphthalene-5-sulfonate attached via a gamma-phosphoamidate bond has been synthesized. both the purine and pyrimidine analogs have fluorescence emission maxima at 460 nm. cleavage of the alpha-beta-phosphoryl bond produces change in both the absorption and fluorescence emission spectra. the fluorescence of the pyrimidine analogs is quenched; cleavage of the alpha-beta-phosphoryl bond of the utp analog produces abo ... | 1979 | 387781 |
| potency of 10 bcg vaccines as evaluated by their influence on the bacillemic phase of experimental airborne tuberculosis in guinea-pigs. | | 1979 | 387796 |
| human plasma angiotensinogen: a review of purification procedures. | the current status of the purification and characterization of human angiotensinogen is reviewed. one problem encountered in the past has been the copurification of a protein with similar porperties. this protein has tentatively been designated alanine-protein. an efficient separation of angiotensinogen and alanine-protein was obtained on a zinc chelate column. alanine-protein has been purified and its amino acid and carbohydrate composition determined. the cooh-terminal amino acid and the nh2-t ... | 1979 | 390363 |
| nucleic acid and protein metabolism in the pancreas, spleen, thymus, lung, cardiac and skeletal muscle and lens of undernourished female rats. | | 1979 | 533871 |
| the isolation and partial characterization of the serum lipoproteins and apolipoproteins of the rainbow trout. | 1. vld (very-low-density), ld (low-density) and hd (high-density) lipoproteins were isolated from the serum of trout (salmo gairdneri richardson). 2. each lipoprotein class resembled that of the human in immunological reactivity, electrophoretic behaviour and appearance in the electron microscope. trout ld lipoprotein, however, was of greater density than human ld lipoprotein. 3. the trout lipoproteins have lipid compositions which are similar to those of the corresponding human components, exce ... | 1978 | 100102 |
| site dependence for topical absorption of nitroglycerin in rats. | | 1978 | 99511 |
| endogenous prostaglandin release contributes directly to coronary artery tone. | an in vitro coronary artery preparation of beef heart was found to synthesize and release continuously large amounts of a prostaglandin of the e type. inhibition of prostaglandin synthesis with aspirin, indomethacin, or eicosa-5,8,11,14-tetraynoic acid induced a sustained contraction of the coronary artery, and pretreatment with indomethacin diminished markedly the output of prostaglandin into the bathing medium. it appears that prostaglandin e1, generated from within the vessel wall itself, may ... | 1975 | 1175084 |
| [orthosympathetic stimulation after hypercapnia in the rat]. | inhalation of a gas mixture : co2, 25% -- o2, 37.5% -- n2, 37.5%, provokes regularly in the light anaesthetized rat, an increase of catecholaminemia of central origin, associated with systemic arterial hypertension. | 1979 | 92225 |
| isoelectric focusing of oligopeptides: detection by specific stains. | by using ultrathin (350 micrometers) polyacrylamide gels, which at the end of the fractionation are pasted to filter paper and dried in an oven at 110 degrees c, and after isoelectric focusing it has been possible to detect oligopeptides in the di- to tetradecapeptide range, which could not be detected by protein staining techniques. this is achieved by developing a series of specific stains for the following amino acids: arg, tyr, his, trp, met and cys. except for met and cys, the detection lim ... | 1979 | 95344 |
| comparative antihypertensive effects and tissue distribution of beta adrenergic blocking drugs. | the precise mechanism of the antihypertensive action of beta adrenergic blocking drugs is not known. both peripheral and central sites of action have been proposed. the comparative antihypertensive actions and tissue distribution of propranolol, pindolol and sotalol were investigated in both normotensive sprague-dawley and spontaneously hypertensive rats. from recordings of concurrent changes in blood pressure and heart rate it was observed that oral or subcutaneous administrations of both propr ... | 1975 | 1151751 |
| alteration of colony-stimulating factor output, endotoxemia, and granulopoiesis in cyclic neutropenia. | cellular and humoral factors involved in the regulation of granulopoiesis were evaluated in two patients with cyclic neutropenia by utilizing the agar-gel marrow culture technique to serially study marrow granulocytic colony-forming capacity (cfc) and the urinary output of colony-stimulating factor (csf). csf output varied inversely with peripheral neutrophil counts and directly with monocyte counts and evidence for infection (endotoxemia and/or staphylococcal abscesses). following autologous in ... | 1976 | 1087533 |
| attenuated, streptomycin-dependent salmonella typhi oral vaccine: potential deleterious effects of lyophilization. | four studies were done with streptomycin-dependent salmonella typhi as an oral, attenuated vaccine. studies 1 and 3 employed freshly harvested vaccine, whereas studies 2 and 4 involved lyophilized vaccine. five to eight doses (3 x 10(10)-10(11) organisms/dose) were given; oral streptomycin (1.0 g) was administered concomitantly in studies 2 and 3, with only two of the doses of vaccine in study 1, and was not given in study 4. no adverse reactions were encountered in 179 vaccinated men, and 94% o ... | 1976 | 1262709 |
| [hypothetical concept: the physiopathological entity of monoaminergic psychoses]. | the importance of amino acid transport across the blood-brain barrier as the limiting factor in the metabolism of monoamines has been emphasized by many recent publications. particularly critical is the transport of tryptophan, since tryptophan hydroxylase is not saturated. this transport is regulated by complex mechanisms, both at the periphery (total and free plasmatic levels and levels of the other essential amino acids) and centraly (by feedback mechanism initiated at the pre- and post-synap ... | 1975 | 1082812 |
| vibrios in acute diarrhoea. | | 1979 | 544501 |
| expression and release of neuroregulators during development: monoamines and neuropeptides. | the turnover of catecholamines (ca) was determined in the adrenal medulla and brain of rat fetuses and pups. in general we found a considerable increase soon after birth. the expression of mrna for ca-synthesizing enzymes was also considerably enhanced in the adrenals shortly after birth. furthermore, we demonstrated increased expression of neuropeptides after birth, increased synthesis of mrna encoding for neuropeptide y in the adrenals 24 h after birth; and considerable activation of the subst ... | 1992 | 1306802 |
| the effects of dopamine on neurones in the nucleus tractus solitarii and on the central control of blood pressure in the rat [proceedings]. | | 1979 | 529063 |
| orofacial tumours induced in rats with radioactive cerium chloride. | | 1979 | 518961 |
| origin and early development of canine circumanal glands. | | 1979 | 517821 |
| [autoradiographic studies on the distribution of 14c-piracetam in the simian brain]. | autoradiography of the brain of the monkey callithrix jacchus 2 and 6 h after oral application of 200 mg 14c-piracetam/kg (2-oxo-pyrrolidine-l-acetamide-2-14c) shows that the drug is preferably concentrated in the cortex of cerebrum and cerebellum. this specific affinity of piracetam which was observed earlier in dog and rat is thus confirmed in the primate and seems to be species independent. beside the dominant cortical concentration there is a characteristic storage of piracetam in many nucle ... | 1978 | 415743 |
| endotoxin tolerance in rats treated with antilymphocyte serum. | rats were treated with rabbit anti-rat lymphocyte serum (als). the subsequent selective immunosuppressive effect on the immune response of thymus dependent antigen was shown by immunization with sheep red blood cells (srbc). endotoxin tolerance could be evoked in als treated animals. this suggests that the establishment of endotoxin tolerance is independent of thymus function and makes it possible to enhance the nonspecific resistance of immunosuppressed patients with a transplanted organ. | 1979 | 395828 |
| [the molecular biology of aging -- 15: structure and function of the genetic apparatus in aging. ii. synthesis and metabolism of rna]. | with aging in different organs there are similar or tissue specific changes of the ribonucleic acids in respect of the quantity, the spectrum of different rna species and the metabolism of rna. among these processes the template function of the chromatin and the behaviour of the rna polymerases are important influencing factors. in aging the sensitivity of the rna synthesis against hormonal stimulation is decreased in the most cases. a survey about the results of many in vivo and in vitro experi ... | 1979 | 395770 |
| [basophils in generalized rash (polák & turk) (author's transl)]. | | 1978 | 309957 |
| the effect of vasectomy on the testes of inbred lewis rats. | by 3 months after surgery, testicular weights were 12-14% below control values in a large series of vasectomized rats of the inbred lewis strain. testicular sperm concentrations in the vasectomized rats were 25-29% below those in the sham-operated animals. although modest in magnitude, these differences were highly significant (p less than 0.001). histometric analysis of testes from a smaller series of vasectomized lewis rats revealed various proportions of abnormal seminiferous tubules. the deg ... | 1978 | 309945 |
| influence of specificity of reagents and of behaviour of cell surface ig on the detection and enumeration of immunoglobulin-bearing lymphocytes in humans. | some parameters likely to influence detection and classification of human b-lymphocytes using anti-immunoglobulin (ig) sera have been investigated. of 20 separate mono- and polyspecific native or conjugated anti-ig sera analysed by a passive haemagglutination technique, 13 exhibited non-specific reactivity. this technique showed no consistent correlation between the titre of individual sera against fab2 and whole igg respectively. the indirect immunofluorescence (if) method applied to detect sur ... | 1978 | 307583 |
| the storage lipids in tangier disease. a physical chemical study. | the physical states and phase behavior of the lipids of the spleen, liver, and splenic artery from a 38-yr-old man with tangier disease were studied. many intracellular lipid droplets in the smectic liquid crystalline state were identified by polarizing microscopy in macrophages in both the spleen and liver, but not in the splenic artery. the droplets within individual cells melted sharply over a narrow temperature range, indicating a uniform lipid composition of the droplets of each cell. howev ... | 1977 | 193870 |
| sequential hepatic histologic and histochemical changes produced by diethylnitrosamine in the rhesus monkey. | six young adult male rhesus monkeys were given diethylnitrosamine ip for 3-5 years. liver biospies were done monthly. after 6 months, biopsy specimens showed individual hepatocytes and small foci of hepatocytes that were intensely positive for glycogen. during the second and later years, larger foci of such cells developed. in sections stained with hematoxylin and eosin, the glycogen-containing hepatocytes generally appeared unusually clear. some hepatocytes, however, had eosinophilic or basophi ... | 1976 | 187798 |
| evidence for species-specific substrate-site-directed inactivation of rabbit adenylate kinase by n6-(6-iodoacetamido-n-hexyl)adenosine 5'-triphosphate. | adenosine 5'-triphosphate (atp) derivatives bearing iodoacetylamino-n-alkyl substituents [(ch2)nnhcoch2i] on n6 were synthesized as potential atp-site-directed irreversible inhibitors of adenylate kinases from rabbit, pig, and carp muscle. when n was 5 no enzyme was progressively inhibited (inactivated) by 1 mm inhibitor under the test conditions (6 h at 0 degrees); when n was 6 the rabbit enzyme was 76% inactivated by 0.79 mm inhibitor whereas the pig and carp enzymes were unaffected by 2.76 mm ... | 1976 | 187748 |
| reversal by concanavalin a of the inhibitory effects of extracellular ca2+ on pinocytosis in amoeba proteus. | when concanavalin a, 1-20 micrograms ml-1 binds to the surface of amoeba proteus the cell's response to ca2+ and to cationic inducers of pinocytosis is strikingly altered. separately, concanavalin a and ca2+ are weak inducers but their combined effects are intense pinocytosis and suppression of the normal inhibitory effect of ca2+ on cation-induced pinocytosis. at high concentrations (greater than 25 micrograms ml-1) the lectin increases cellular uptake and binding of 45ca in the cell surface bu ... | 1992 | 1595356 |
| genetically induced inner ear degeneration. a structural and functional study. | the jerker mouse mutant has an autosomal recessive mutation, which in homozygotes results in early postnatal degeneration of the sensory epithelia in both the cochlea and vestibulum. an age-related consecutive functional and morphological analysis of the cochlea has been performed in homozygote (je/je) and heterozygote (je/+) jerker mice. cochlear function was determined with auditory brainstem response (abr) technique. heterozygotes were regularly tested to a final age of 12 months. with increa ... | 1992 | 1636414 |
| conduction velocity distribution of afferent fibers innervating the rat urinary bladder. | the conduction velocities of individual afferent fibers innervating the rat urinary bladder were determined by the antidromic stimulation of dorsal roots while recording from bladder postganglionic nerves. conduction velocities ranged from 0.5 to 21.0 m/s; 70% of the velocities were less than 2.5 m/s. the distribution within the dorsal roots was ipsilateral with 84% in l6 and 16% in s1. neuroanatomical tracing with horseradish peroxidase applied to individual bladder postganglionic nerves result ... | 1990 | 1698508 |
| analysis of high psa n-cam expression during mammalian spinal cord and peripheral nervous system development. | using a monoclonal antibody that recognizes specifically a high polysialylated form of n-cam (high psa n-cam), the temporal and spatial expression of this molecule was studied in developing spinal cord and neural crest derivatives of mouse truncal region. temporal expression was analyzed on immunoblots of spinal cord and dorsal root ganglia (drgs) extracts microdissected at different developmental stages. analysis of the ratio of high psa n-cam to total n-cam indicated that sialylation and desia ... | 1991 | 1769342 |
| fluorescence studies on the interaction of the tumor promoter phorbol myristate acetate and related compounds with rat liver plasma membranes. | the interaction of the potent tumor-promoting agent phorbol myristate acetate (pma) with purified rat liver plasma membranes suspended in phosphate-buffered saline (pbs), ph 7.4, was studied by fluorescence spectrophotometry. exposure of membranes to pma caused up to 21% decrease of the native membrane emission, i.e. the fluorescence of both tryptophan and tyrosine, compared to non-treated membranes. the decrease in the membrane emission varied with both the pma and the membrane concentration. t ... | 1976 | 187350 |
| virus diseases of bone. do they exist? the neuhauser lecture. | | 1976 | 179334 |
| design of site-specific protein drugs. | the ability to clone and express proteins at high levels of abundance and purity has led to their increasing consideration as therapeutic agents. the use of various protein engineering and recombinant dna techniques has enabled progression to where proteins can be tailored so that they have not only the ability to interact with a unique (extra)cellular component in order to produce a pharmacological effect, but their structure can be altered so as to achieve a selective biological disposition. t ... | 1991 | 1815386 |
| early cellular events in the response of mice to sheep red blood cells reflected by changes in the spleen level of cyclic amp. | | 1975 | 165767 |
| processing of the glycoprotein of feline immunodeficiency virus: effect of inhibitors of glycosylation. | the processing and transport of the envelope glycoprotein complex of feline immunodeficiency virus (fiv) in the persistently infected crandell feline kidney (crfk) cell line were investigated. pulse-chase analyses revealed that the glycoprotein is synthesized as a precursor with an mr of 145,000 (gp145) and is quickly trimmed to a molecule with an mr of 130,000 (gp130). treatment of gp130 with endoglycosidase h (endo h) resulted in a protein with an mr of 75,000, indicating that nearly half the ... | 1991 | 1847441 |
| incorporation of 14c-thymidine into liver and brain dna of protein-deficient rats. | the authors studied the effect of a protein-free diet on 14c-thymidine incorporation into rat liver dna in vivo and found, after 2-3 weeks, a marked decrease in uptake of the radioactive base into the liver dna, followed by a decrease in the proportion of dna in liver cell homogenates. the total nuclear count/mg liver tissue displayed an increase during protein depletion, except for the 5th week, when a decrease was recorded. the incorporation of 14c thymidine into the brain dna likewise display ... | 1978 | 148059 |
| elementary particles in isolated rat liver and spleen nuclear membranes. | elementary spherical particles similar to those described in the mitochondria are found in isolated rat liver and spleen nuclear membranes. the particles are characterized by electron microscopy of sections and preparations negatively stained with phosphotungstic acid or with the atpase histochemical reaction product. the particles exhibit adenosine triphosphatase activity and have a mushroom-like shape with a sphere about 85-90 angstrom in diameter attached to inner face of the inner nuclear me ... | 1975 | 126876 |
| prognostic value of rhesus blood groups in oral squamous cell carcinomas. | in the current study of the prognosis of all patients (n equals 70) with squamous cell carcinomas (scc) of floor of mouth in norway during the period 1963 to 1972, the authors found that patients with rhesus (rh) (d)-negative blood group had significantly poorer prognosis (mean 5-year survival, 8%) than patients with rh (d)-positive blood group (5-year survival, 30%) (p equals 0.04). this extends the authors' previous observations in another group of oral cancer patients. the authors do not know ... | 1991 | 1913459 |
| functional and electrophysiological effects of oxidant stress on isolated ventricular muscle: a role for oscillatory calcium release from sarcoplasmic reticulum in arrhythmogenesis? | the aim was to investigate the cellular basis of oxidant stress induced arrhythmias by studying the influence of oxidant stress on the contractile and electrophysiological function of isolated cardiac muscle. | 1991 | 1913755 |
| dietary fats and properties of endoplasmic reticulum: i. dietary lipid induced changes in composition of microsomal membranes in liver and gastroduodenal mucosa of rat. | rats were fed for four weeks with different lipid diets to determine the effects on the endoplasmic reticulum membranes of the liver and on the postmitochondrial supernatant fraction of the gastroduodenal mucosa. the diets contained cholesterol, cacao butter, olive oil, and these in combination. the results showed that dietary lipids were able to modify the composition of the hepatic endoplasmic reticulum and, to a lesser extent, that of postmitochondrial fraction of gastroduodenal mucosa. cacao ... | 1975 | 1160521 |
| [progresses in the development of antimycotic effective substances (author's transl)]. | comparative review of widely used classic and modern antimycotic agents - polyene-group, griseofulvin, tolnaphthate, 5-fluorcytosin and the new imidazolyl-group - in view of antimycotic, pharmacocinetic and therapeutic properties. | 1975 | 1184097 |
| fatal ureaplasmal pneumonia and sepsis in a newborn infant. | ureaplasma urealyticum was isolated in pure culture from blood tracheal aspirate and lung tissue in a newborn infant, who died of a severe pneumonia within 48 h after birth. the clinical course was characterized by persistent pulmonary hypertension of the newborn (pphn). post-mortem examination revealed extensive hyaline membrane formation combined with signs of inflammation in both lungs. the clinical and histopathological picture resembled that of early onset group b haemolytic streptococcal p ... | 1991 | 1959541 |
| low tumorigenicity of canine cells transformed by the human cytomegalovirus. | dog embryo kidney cells transformed by the human cytomegalovirus (hcmv) were obtained after non-permissive infection or transfection with viral dna digested by restriction endonuclease ecor i. the transformed cells, growing rapidly and showing an unlimited division potential, could use medium with only 2% serum for growth, contained nuclear virus antigens, and formed small colonies (less than 0.2 mm) in agarose. from 40 mice inoculated with transformed canine cells, only one eventually developed ... | 1990 | 1964816 |
| contrast and electrolyte dynamics of the intravenous pyelogram. ii. disparities in serum and urine concentrations of meglumine and anion. | intravenous or intra-aortic injections of meglumine iothalamate in dogs indicate a slightly different tissue distribution and excretory pattern of the meglumine cation and the iothalamate anion. differences in serum and urinary concentrations of these ions suggest some cellular penetration of the meglumine anion. in addition, there seems to be some evidence for both tubular absorption and tubular excretion of meglumine as a minor component in the renal excretion of this ion. no significant diffe ... | 1975 | 1184320 |
| hypoglycemia risk during exercise after intramuscular injection of insulin in thigh in iddm. | the influence of bicycle exercise (60% of w170 [working capacity at a pulse rate of 170 beats/min]; 40 min) on the absorption of 125i-labeled fast-acting insulin (10 u; actrapid human insulin) after intramuscular compared with subcutaneous injection in the thigh was studied on 2 consecutive days in 10 insulin-dependent diabetes mellitus (iddm) patients. insulin absorption was measured as disappearance of radioactivity (1st-order elimination rate constants) by continuous external monitoring and a ... | 1990 | 2190773 |
| mutations in the trfa replication gene of the broad-host-range plasmid rk2 result in elevated plasmid copy numbers. | mutated forms of trfa, the replication protein gene of plasmid rk2, that support a minimal rk2 origin plasmid in escherichia coli at copy numbers up to 23-fold higher than normal have been isolated. six such high-copy-number (copy-up) mutations were mapped and sequenced. in each case, a single base transition led to an amino acid substitution in the trfa protein primary sequence. the six mutations affected different residues of the protein and were located within a 69-base-pair region encoding 2 ... | 1990 | 2193921 |
| presence of cellular retinol and retinoic acid binding proteins in human uterus. | human uterine cells contain two separate soluble proteins, one binding retinol and one binding retinoic acid with high specifity. this suggests that both retinol and retinoic acid may be required for normal uterine function. | 1975 | 1184294 |
| interrelationships of dietary sodium, potassium and chloride on growth in young swine. | in a preliminary experiment, pigs fed a purified diet gained at the same rate as pigs fed a natural ingredient diet if the purified diet was formulated to provide 150% of the na, k and cl requirements. subsequent experiments were designed to determine the interrelationships of dietary na, k and cl. pigs were fed diets with na (.03% to .60%), k (.1% to 1.0%) and cl (.03% to .60%) levels arranged in a three-factor central composite design. over the range of na levels, predicted gain increased by . ... | 1990 | 2211403 |
| [effect of enalapril on heart rate, arterial blood pressure and exocrine pancreatic secretion in the alert dog]. | a changed exocrine pancreatic secretion could be a pathogenetic factor of an acute pancreatitis after administration of angiotensin-converting enzyme (ace) inhibitors. in six conscious dogs with gastric and duodenal thomas fistulas we studied the effect of an intravenous (iv.) bolus injection of 10 mg enalaprilat, an intraduodenal (id.) bolus injection of 20 and 40 mg enalapril (e.), and 0.15 m nacl (20 ml iv., resp., id.) on pancreatic bicarbonate- and protein output in response to secretin (20 ... | 1990 | 2238764 |
| the detection of antibodies in human and animal filariases by counterimmunoelectrophoresis with dirofilaria immitis antigens. | counterimmunoelectrophoresis revealed the presence of precipitin antibody in all of 6 dogs and the 1 cat infected with dirofilaria immitis and in the serum of 17 of 24 individuals living in a setting of hyperendemic subperiodic bancroftian filariasis. antigens used in the test were prepared from microfilariae and adult male d. immitis. some humans and animals had antibodies to both antigens while others had antibodies against microfilariae or adult worms only. the presence of soluble circulating ... | 1976 | 1262695 |
| [our experience in spot large-frame fluorography in the study of the upper regions of the gastro-intestinal tract]. | | 1976 | 1266007 |
| [effects of hypokinesia on cardiac activity in rats with high and low spontaneous motor activity]. | hypokinesia effects on cardiac contractivity and vegetative regulation were studied in rats with high and low spontaneous motor activity. it was shown that hypokinesia promotes depression of the stroke volume and decrease of sympathetic nervous activity in rats with low motor activity. at the same time, in rats with high motor activity these parameters were not significantly affected by hypokinesia. the conclusion is made about a relationship between resistance to hypokinesia and spontaneous mot ... | 1992 | 1301100 |
| adherence and survival properties of an epidemic methicillin-resistant strain of staphylococcus aureus compared with those of methicillin-sensitive strains. | the adherence of epidemic methicillin-resistant staphylococcus aureus-1 (emrsa-1) to hep2 cells and fibronectin and its survival on formica blocks were compared with the equivalent properties of methicillin-sensitive strains of s. aureus. emrsa-1 bound to hep2 cells and fibronectin significantly less than the other strains of s. aureus tested, but survival on formica blocks was similar for all strains except the 'oxford' s. aureus, which survived poorly. | 1990 | 2374157 |
| gap-43 mrna localization in the rat hippocampus ca3 field. | gene expression of the axonal growth-associated protein, gap-43, has been studied in the adult rat brain by in situ hybridization histochemistry. this protein is synthesized at high levels in neuronal somata in immature and regenerating neurons, but after establishment of mature synaptic relations its synthesis generally declines sharply, thus providing a marker denoting propensity for exhibiting synaptic plasticity. detailed examination of the distribution of mrna for gap-43 in rat hippocampus ... | 1992 | 1317499 |
| stimulation of rat pancreatic tumoral ar4-2j cell proliferation by pituitary adenylate cyclase-activating peptide. | in the present work the effects of the novel neuropeptide pituitary adenylate cyclase activating peptide (pacap) on both ar4-2j cell growth and the modulation of ornithine decarboxylase activity were investigated. both pacap38 and the amidated form pacap27 caused a concentration-dependent stimulation of ar4-2j cell growth; the maximal increase was seen at 1 nmol/l (30% above control, p less than 0.01) with a half-maximal effect at 0.01 nmol/l. ornithine decarboxylase activity was also increased ... | 1992 | 1323494 |
| linkage analysis of spinal muscular atrophy. | linkage data between four markers on chromosome 5 confirm and extend our previous studies that localized the mutation in spinal muscular atrophy to 5q11.2-q13.3. localization of d5s6 by in situ hybridization refines the mapping of the defective gene to the region 5q12.2-q13. we also report the use of a highly informative pcr-based polymorphism with five alleles. this rflp will be particularly useful for prenatal diagnosis where only old tissue samples from affected individuals are available. the ... | 1992 | 1346777 |
| modulation of adhesion molecules on human large granular lymphocytes by interleukin-2 in vivo and in vitro. | the modulation of adhesion molecules on human large granular lymphocytes (lgl) by interleukin (il)-2 was investigated both in vivo and in vitro. intercellular adhesion molecule-1 (icam-1; cd54) expression increased on lgl of cancer patients receiving il-2 adoptive immunotherapy. icam-1 expression on lgl isolated by percoll gradient centrifugation, lgl purified, and expanded by adherence to plastic surfaces and lgl identified by leu 19 (cd56) monoclonal antibody were increased significantly in re ... | 1992 | 1347485 |
| some physico-chemical properties of the rigid form of the sendai virus nucleocapsid. | the effect of some dissociation agents (sds, beta-mercaptoethanol, urea, edta) on the rigid form of the sendai virus nucleocapsid was studied. polyacrylamide gel electrophoresis in the presence of lytic mixture (1% sds, 2% beta-mercaptoethanol, 5 m urea, for 2 min at 100 degrees c) revealed two types of polypeptide subunits (mol. wts. 46,000 and 14,000), as well as the dissociation in the presence of 0.1% sds only. the edta treatment leads to a disorganization of the protein part (10(-2) m) or o ... | 1989 | 2556836 |
| immunogenicity of a killed hepatitis a vaccine in seronegative volunteers. | 32 volunteers who lacked antibody to hepatitis a virus (hav) received, at intervals of one month, three injections of a killed vaccine made from hav propagated in diploid human fibroblast cell cultures. in 16 the vaccine was coupled with aluminium hydroxide as adjuvant. the serconversion rates measured by radioimmunoassay, with and without adjuvant respectively, were 13% and 25% at four weeks, 81% and 89% at eight weeks, and 94% and 100% at twelve weeks. four weeks after the third injection all ... | 1989 | 2565998 |
| influence of low-temperature storage and glucose starvation on growth recovery in escherichia coli rela and rela+ strains. | to study the influence of microgravity on bacterial growth behavior during a space mission, the special experimental conditions and the hardware environment necessitate storage of cells at low temperature, and permit a relatively short experimental period. before this experimental period, cells have to recover their condition of steady-state growth, because it is only in this condition that the growth behavior of the flight and ground populations can be adequately compared. to meet these require ... | 1991 | 1367237 |
| characterization of guanosine diphospho-d-mannose dehydrogenase from pseudomonas aeruginosa. structural analysis by limited proteolysis. | alginate is believed to be a major virulence factor in the pathogenicity of pseudomonas aeruginosa in the lungs of patients suffering from cystic fibrosis. guanosine diphospho-d-mannose dehydrogenase (gdpmannose dehydrogenase, ec 1.1.1.132) is a key enzyme in the alginate biosynthetic pathway which catalyzes the oxidation of guanosine diphospho-d-mannose (gdp-d-mannose) to gdp-d-mannuronic acid. in this paper, we report the structural analysis of gmd by limited proteolysis using three different ... | 1992 | 1370473 |
| biochemical and functional characterization of the leucocyte tyrosine phosphatase cd45 (cd45ro, 180 kd) from human neutrophils. in vivo upregulation of cd45ro plasma membrane expression on patients undergoing haemodialysis. | the biochemical and functional characterization, and the regulation of plasma membrane expression of the leucocyte tyrosine phosphatase cd45, have been investigated in neutrophils from healthy donors and patients undergoing haemodialysis. cd45 proteins of 180 kd and 130-150 kd were precipitated from neutrophils from both healthy subjects and haemodialysed patients. prolonged storing, as well as trypsin treatment of samples containing the 180-kd cd45 protein, generated the 130-150-kd polypeptides ... | 1992 | 1370931 |
| the behaviour of sensory ganglia in relation to their environment. | the nodose ganglion was removed from rats and transplanted into the denervated tongue and into the denervated or innervated sternocleidomastoid muscle. in another procedure the ganglion was sutured to the distal hypoglossal or sciatic nerve. the ganglia failed to develop motor end-plates on denervated muscle. they did, however, induce formation of taste buds on the lingual epithelium. the regenerated nerve fibres ramified extensively within the connective tissue, invading perivascular tissue and ... | 1989 | 2606773 |
| molecular phylogenetic inference from saber-toothed cat fossils of rancho la brea. | a method for the successful extraction of sequestered cellular dna from 14,000-year-old fossil bones was developed and applied to asphalt-preserved specimens of the extinct saber-toothed cat, smilodon fatalis. two distinct gene segments, the mitochondrial gene for 12s rrna and nuclear fla-i (the feline class i major histocompatibility complex gene), from three different individual fossil specimens were cloned and sequenced after pcr amplification. comparison of fossil-derived dna sequences to ho ... | 1992 | 1409696 |
| sleep organization and energy expenditure of breast-fed and formula-fed infants. | sleep organization of infants may be influenced by differences in nutrient intakes from human milk and formula. because sleep/awake and sleep stage patterns affect energy expenditure, we hypothesized that differences in sleep organization between breast-fed and formula-fed infants might account in part for differences in energy expenditure between feeding groups. sleep stages and cycling of 4-mo-old breast-fed (n = 10) formula-fed (n = 10) infants were studied with simultaneous measurements of e ... | 1992 | 1480450 |
| polymorphism in the rd (d6s45) gene. | the rd (d6s45) gene in the class iii region of the hla major histocompatibility complex encodes a protein normally containing 24 consecutive basic-acidic dipeptide repeats. we determined the frequency of variations in the number of repeats by use of the polymerase chain reaction. of 107 subjects 7 (3.3%) carried genes encoding 22 or 23 repeats. there was no difference in the frequency of such polymorphisms between normal individuals and those with systemic lupus erythematosus, a disease associat ... | 1992 | 1534063 |
| diagnosis of sickle cell disease in chronically transfused patients. | standard electrophoretic methods for the diagnosis of hemoglobinopathies are confounded in individuals chronically transfused. we present the accurate diagnosis of sickle cell disease in two such transfused patients by the application of polymerase chain reaction technology to analyze patient's hemoglobin beta-chain genes directly. | 1992 | 1550267 |
| 3-o-alkylascorbic acids as free radical quenchers. 3. protective effect on coronary occlusion-reperfusion induced arrhythmias in anesthetized rats. | structural modification of ascorbic acid by substitution of the 3-hydroxy group with lipophilic moieties has allowed the development of agents for treating reperfusion injury. these ascorbic acid derivatives inhibited lipid peroxidation, and some of them also reduced coronary reperfusion-induced arrhythmias in anesthetized rats. we found that 3-o-[(dodecylcarbonyl)methyl]ascorbic acid (8) was protective against reperfusion injury without directly influencing hemodynamics. 2-o-octadecylascorbic a ... | 1992 | 1578489 |
| the effect of age on motor neurone death following axotomy in the mouse. | the ability of mouse motor neurones to survive axotomy during the first month of life was studied. the motor neurones that lie in the dorsolateral columns of spinal segments c7 and c8 and supply the flexor muscles of the forepaw were axotomized by cutting and removing part of the median and ulnar nerves above the elbow. the number and position of cell bodies with axons in these nerves were confirmed by retrograde labelling of the cut axons with horseradish peroxidase. the ability of these neuron ... | 1991 | 1769343 |
| mutagenesis by the anti-tumour drug nitracrine in escherichia coli. | the antitumour drug nitracrine [1-nitro-9-(dimethylaminopropylamino)acridine], known to be a potent frameshift mutagen in strains of salmonella typhimurium, also strongly reverts the lacz19124 frameshift marker in escherichia coli. the results in e. coli indicate that nitracrine causes dna damage which can be excised by the uvra,b,c excinuclease, can generate mutations by a reca-dependent mechanism, and gives enhanced yields of mutants when plasmid pkm101 is present. despite these observations, ... | 1989 | 2685595 |
| mutagenesis by the anti-tumour drug nitracrine in escherichia coli. | the antitumour drug nitracrine [1-nitro-9-(dimethylaminopropylamino)acridine], known to be a potent frameshift mutagen in strains of salmonella typhimurium, also strongly reverts the lacz19124 frameshift marker in escherichia coli. the results in e. coli indicate that nitracrine causes dna damage which can be excised by the uvra,b,c excinuclease, can generate mutations by a reca-dependent mechanism, and gives enhanced yields of mutants when plasmid pkm101 is present. despite these observations, ... | 1989 | 2685595 |
| characterization of the human gene for a newly discovered carbonic anhydrase, ca vii, and its localization to chromosome 16. | six carbonic anhydrase (ca) isozymes (ca i-vi) in mammals and other amniotes have been described. we have isolated an additional ca gene from a human genomic library and designated its putative product carbonic anhydrase vii (ca vii). the gene is approximately 10 kb long and contains seven exons and six introns found at positions identical to those determined for the previously described ca i, ca ii, and ca iii genes. the finding of a 17-bp gt-rich segment in a position 28 bp downstream of the p ... | 1991 | 1783392 |
| growth arrest induced by transforming growth factor beta 1 is accompanied by protein phosphatase activation in human keratinocytes. | protein phosphorylation and dephosphorylation are involved in regulation of cell growth. we tested the hypothesis that the growth inhibitory effect of transforming growth factor beta 1 (tgf-beta 1) involves activation of protein phosphatases. exposure of human keratinocytes in culture to 400 pm tgf-beta 1 for 48 h led to 80% inhibition of dna synthesis as measured by nuclear labeling. incubation of cultured keratinocytes with 400 pm tgf-beta 1 rapidly activated (within 30 min) protein serine/thr ... | 1991 | 1847373 |
| involvement of protein kinase c in the ca2+-dependent vesicular release of gaba from central and enteric neurons of the guinea pig. | the involvement of protein kinase c (pkc) in the release of endogenous gamma-aminobutyric acid (gaba) was studied using slices of deep cerebellar nucleus and strips of small intestine from the guinea pig. 12-o-tetradecanoylphorbol 13-acetate (tpa), but not 4 alpha-phorbol-12,13-didecanoate (4 alpha-pdd), potentiated the high k+-evoked release of gaba from both preparations in the presence of tetrodotoxin. ouabain evoked the release of gaba from both preparations, and this release was not altered ... | 1989 | 2706529 |