| use of gas chromatography for detecting ornithine and lysine decarboxylase activity in bacteria. | a gas-liquid chromatography (glc) procedure for the detection of l-ornithine and l-lysine decarboxylase (ec 4.1.1.17 and ec 4.1.1.18, respectively) activities of bacteria was developed and evaluated against møller's method, a conventional biochemical test. cultures were incubated for 2 to 4 h in a simple growth medium and tested by glc for putrescine and cadaverine, the direct decarboxylation products of ornithine and lysine, respectively. results obtained with various enterobacteriaceae, pseudo ... | 1973 | 4751799 |
| lipopolysaccharide of rhodospirillum salinarum 40: structural studies on the core and lipid a region. | the structural elucidation of lipid a of the cell wall lipopolysaccharide (lps) of rhodospirillum salinarum 40 by chemical methods and laser desorption mass spectrometry revealed the presence of a mixed lipid a composed of three different 1,4'bisphosphorylated beta (1 --> 6)-linked backbone hexosaminyl-hexosamine disaccharides, i.e. those composed of glcn --> glcn, 2,3-diamino-2,3-dideoxy-d-glc-(dag --> dag, and dag --> glcn. lipid a of r. salinarum contained preferentially 3-oh-18:0 and 3-oh-14 ... | 1995 | 7487334 |
| influence of charge and polarity on the redox potentials of high-potential iron-sulfur proteins: evidence for the existence of two groups. | we have investigated the hipips from ectothiorhodospira vacuolata (iso-1 and iso-2), chromatium vinosum, rhodocyclus gelatinosus, rhodocyclus tenuis (strain 2761), rhodopila globiformis, and rhodospirillum salinarum (iso-2) by direct electrochemistry. using a glassy carbon electrode with a negatively charged surface, direct, unpromoted electrochemistry is possible with the positively charged hipips. with the negatively charged hipips, the positively charged and flexible bridging promoter poly(l- ... | 1995 | 7578075 |
| reversible super-reduction of the cubane [4fe-4s](3+;2+;1+) in the high-potential iron-sulfur protein under non-denaturing conditions. epr spectroscopic and electrochemical studies. | the reversible 2 x 1 e- reduction of the cubane cluster from oxidized to reduced to super-reduced states ([4fe-4s]3+<-->[4fe-4s]2+<-->[4fe-4s]1+) was studied in high-potential iron-sulfur proteins (hipips). super-reduction to the 1+ state was not observed in any of the seven hipips tested during cyclic voltammetry (down to -0.95 v). however, equilibration at low potential (ph 7.5) of rhodopila globiformis hipip yields a transient peak around -0.47 v due to the oxidation of super-reduced hipip ad ... | 1995 | 7588720 |
| anaerobic bacteria from hypersaline environments. | strictly anaerobic halophiles, namely fermentative, sulfate-reducing, homoacetogenic, phototrophic, and methanogenic bacteria are involved in the oxidation of organic carbon in hypersaline environments. to date, six anaerobic fermentative genera, containing nine species, have been described. two of them are homoacetogens. six species belong to the family haloanaerobiaceae, as indicated by their unique 16s rrna oligonucleotide sequences. desulfohalobium retbaense and desulfovibrio halophilus repr ... | 1994 | 8177169 |
| the electron transport system of the halophilic purple nonsulfur bacterium rhodospirillum salinarum. 1. a functional and thermodynamic analysis of the respiratory chain in aerobically and photosynthetically grown cells. | plasma membranes isolated from cells of the halophilic purple nonsulfur bacterium rhodospirillum salinarum grown in light or in the dark were examined. membranes isolated from cells grown aerobically in the dark contained three b-type and two c-type membrane-bound cytochromes with em,7 of +180, +72 and -5 mv (561-575 nm), and +244 and +27 mv (551-540 nm), respectively. conversely, membranes isolated from cells grown anaerobically in the light contained two b-type and five c-type haems with em,7 ... | 1997 | 9297468 |
| reclassification of species of the spiral-shaped phototrophic purple non-sulfur bacteria of the alpha-proteobacteria: description of the new genera phaeospirillum gen. nov., rhodovibrio gen. nov., rhodothalassium gen. nov. and roseospira gen. nov. as well as transfer of rhodospirillum fulvum to phaeospirillum fulvum comb. nov., of rhodospirillum molischianum to phaeospirillum molischianum comb. nov., of rhodospirillum salinarum to rhodovibrio salexigens. | the 165 rdna sequence of rhodospirillum mediosalinum was determined and compared with corresponding sequences from other spiral-shaped purple non-sulfur bacteria classified as or related to the genus rhodospirillum in the alpha subclass of the proteobacteria. sequence similarities separate the currently recognized rhodospirillum species into five different groups with no more than 91% sequence similarity, clearly indicating the necessity to recognize these groups as different genera. major diagn ... | 1998 | 9734033 |
| [the structural diversity of lipid a from gram-negative bacteria]. | the majority of gram-negative bacteria are pathogenic to humans and animals. lipopolysaccharide (lps) is the most biologically active component of these microorganisms. this compound is also called endotoxin to emphasize its negative impact on a macroorganism. lipid a, one of the three structural components of the lps molecule, is responsible for the pathophysiological effects associated with gram-negative bacteria infections. although lipid a is considered the conservative component of endotoxi ... | 2007 | 17369779 |
| [have we made advances in the surgical treatment of bronchial carcinomas?]. | | 2015 | 4302308 |
| usual form of bayes' theorem and "common names". | | 2013 | 3831642 |
| amino acid sequences of two high-potential iron-sulfur proteins (hipips) from the moderately halophilic purple phototrophic bacterium, rhodospirillum salinarum. | the amino acid sequences of two very different high-potential iron-sulfur protein (hipip) isozymes have been determined from the moderately halophilic purple phototrophic bacterium, rhodospirillum salinarum. iso-1 hipip, which is monomeric and contains 57 amino acid residues, is most similar to the thiobacillus ferrooxidans iron-oxidizing enzyme (45% identity and a 6-residue deletion). on the other hand, iso-2 hipip, which is isolated as an oligomer, contains a peptide chain with 54 amino acid r ... | 1999 | 10462450 |
| limimonas halophila gen. nov., sp. nov., an extremely halophilic bacterium in the family rhodospirillaceae. | a novel, gram-staining-negative, non-pigmented, rod-shaped, strictly aerobic, extremely halophilic bacterium, designated strain ia16(t), was isolated from the mud of the hypersaline lake aran-bidgol, in iran. cells of strain ia16(t) were not motile. growth occurred with 2.5-5.2 m nacl (optimum 3.4 m), at ph 6.0-8.0 (optimum ph 7.0) and at 30-50 °c (optimum 40 °c). phylogenetic analysis based on 16s rrna gene sequences indicated that strain ia16(t) belonged in the family rhodospirillaceae and tha ... | 2013 | 22904212 |
| [untraditional vacation for disabled and nurses. interview by siv barstad]. | | 2006 | 1489619 |
| fodinicurvata sediminis gen. nov., sp. nov. and fodinicurvata fenggangensis sp. nov., poly-beta-hydroxybutyrate-producing bacteria in the family rhodospirillaceae. | two gram-negatively stained, facultatively anaerobic, non-motile, vibrioid and rod-shaped, chemoheterotrophic bacterial strains, designated yim d82(t) and yim d812(t), were isolated from a salt mine in yunnan, south-west china. dna-dna hybridization, genomic dna g+c content and phylogenetic analyses based on 16s rrna gene sequences divided the two isolates into two distinct genospecies that were also clearly differentiated by fatty acid profiles, carbon source utilization patterns, antibiotic su ... | 2009 | 19622654 |
| plasmid transfer and susceptibility to antibiotics in the halophilic phototrophs rhodovibrio salinarum and rhodothalassium salexigens. | the present study defines a series of genetic procedures to be used for molecular studies in photosynthetic halophilic species such as rhodovibrio salinarum and rhodothalassium salexigens. in both species, the minimal inhibitory concentrations for the antibiotics tetracycline, rifampicin, chloramphenicol, spectinomycin, streptomycin, and kanamycin were determined. in addition, conjugal transfer of incp and incq plasmids from escherichia coli was demonstrated and the resistance markers expressed ... | 2001 | 11287156 |
| on the role of high-potential iron-sulfur proteins and cytochromes in the respiratory chain of two facultative phototrophs | the capability of high potential iron-sulfur proteins (hipips) and soluble cytochromes to shuttle electrons between the bc1 complex and the terminal oxidase in aerobically grown cells of rhodoferax fermentans and rhodospirillum salinarum, two facultative phototrophs, was evaluated. in rs. salinarum, hipip and a c-type cytochrome (alpha-band at 550 nm, em,7=+290 mv) are both involved in the electron transfer step from the bc1 complex to the terminal oxidase. kinetic studies indicate that cytochro ... | 1999 | 10076014 |
| the respiratory chain of the halophilic anoxygenic purple bacterium rhodospirillum sodomense. | the halophilic purple nonsulfur bacterium rhodospirillum sodomense has been previously described as an obligate phototroph that requires yeast extract and a limited number of organic compounds for photoheterotrophic growth. in this work, we report on chemoheterotrophic growth of r. sodomense in media containing either acetate or succinate supplemented with 0.3-0.5% yeast extract. plasma membranes isolated from cells grown aerobically in the dark contained three b-type and three c-type membrane-b ... | 1998 | 9799287 |