| phylogenetic positions of novel aerobic, bacteriochlorophyll a-containing bacteria and description of roseococcus thiosulfatophilus gen. nov., sp. nov., erythromicrobium ramosum gen. nov., sp. nov., and erythrobacter litoralis sp. nov. | we analyzed the 16s ribosomal dnas of three obligately aerobic, bacteriochlorophyll a-containing bacteria, "roseococcus thiosulfatophilus," "erythromicrobium ramosum," and new isolate t4t (t = type strain), which was obtained from a marine cyanobacterial mat. "roseococcus thiosulfatophilus" is a member of the alpha-1 subclass of the proteobacteria and is moderately related to rhodopila globiformis, thiobacillus acidophilus, and acidiphilium cryptum (level of sequence similarity, 90%). "erythromi ... | 1994 | 7520734 |
| influence of charge and polarity on the redox potentials of high-potential iron-sulfur proteins: evidence for the existence of two groups. | we have investigated the hipips from ectothiorhodospira vacuolata (iso-1 and iso-2), chromatium vinosum, rhodocyclus gelatinosus, rhodocyclus tenuis (strain 2761), rhodopila globiformis, and rhodospirillum salinarum (iso-2) by direct electrochemistry. using a glassy carbon electrode with a negatively charged surface, direct, unpromoted electrochemistry is possible with the positively charged hipips. with the negatively charged hipips, the positively charged and flexible bridging promoter poly(l- ... | 1995 | 7578075 |
| reversible super-reduction of the cubane [4fe-4s](3+;2+;1+) in the high-potential iron-sulfur protein under non-denaturing conditions. epr spectroscopic and electrochemical studies. | the reversible 2 x 1 e- reduction of the cubane cluster from oxidized to reduced to super-reduced states ([4fe-4s]3+<-->[4fe-4s]2+<-->[4fe-4s]1+) was studied in high-potential iron-sulfur proteins (hipips). super-reduction to the 1+ state was not observed in any of the seven hipips tested during cyclic voltammetry (down to -0.95 v). however, equilibration at low potential (ph 7.5) of rhodopila globiformis hipip yields a transient peak around -0.47 v due to the oxidation of super-reduced hipip ad ... | 1995 | 7588720 |
| phylogenetic position of gluconobacter species as a coherent cluster separated from all acetobacter species on the basis of 16s ribosomal rna sequences. | the 16s rrna sequences from the gluconobacter species g. asaii, g. cerinus and g. frateurii were determined and compared with homologous sequences from published databases and sequences of g. oxydans and acetobacter species previously described [sievers, m., ludwig, w. and teuber, m. (1994) system. appl. microbiol. 17, 189-196]. the gluconobacter species have unique 16s rrna sequences and exhibit sequence similarity values of 97.4 to 99.1%, corresponding to 36 to 14 base differences. the phyloge ... | 1995 | 7705603 |
| amino acid sequence of a high redox potential ferredoxin (hipip) from the purple phototrophic bacterium rhodopila globiformis, which has the highest known redox potential of its class. | rhodopila globiformis hipip has a redox potential (ca. 450 mv) that is 100 mv higher than any other known iron-sulfur protein. the amino acid sequence contains 57 residues and can be aligned with that of thiobacillus ferrooxidans without any insertions or deletions and is 51% identical. rp. globiformis hipip is also similar to that of rhodocyclus tenuis, but six- and two-residue gaps must be postulated and there is only 37% identity. most of the amino acid residues near the iron-sulfur cluster a ... | 1993 | 8215406 |
| molecular structure of a high potential cytochrome c2 isolated from rhodopila globiformis. | unlike their mitochondrial counterparts, the c-type cytochromes typically isolated from photosynthetic nonsulfur purple bacteria display a wide range of oxidation-reduction potentials. here we describe the x-ray crystallographic analysis of the cytochrome c2 isolated from rhodopila globiformis. this particular c-type cytochrome was selected for study because of its anomalously high redox potential of +450 mv. crystals employed in the investigation belonged to the space group i4(1) with unit cell ... | 1996 | 8809072 |
| acidisphaera rubrifaciens gen. nov., sp. nov., an aerobic bacteriochlorophyll-containing bacterium isolated from acidic environments. | four strains of aerobic, mesophilic, acidophilic bacteria that produced bacteriochlorophyll (bchl) a were isolated from acidic hot springs and mine drainage. the characteristics of the four isolates were almost identical. the isolates were strictly aerobic and chemo-organotrophic. they were gram-negative, non-motile cocci and coccobacilli, formed salmon-pink colonies on solidified media and produced bchl a and carotenoids only under aerobic growth conditions. the cells also produced small amount ... | 2000 | 10939661 |
| genetic characterization of microbial communities living at the surface of building stones. | the aim of the present study was to reveal the microbial genetic diversity of epilithic biofilms using a dna-based procedure. | 2009 | 19552772 |
| acidisoma tundrae gen. nov., sp. nov. and acidisoma sibiricum sp. nov., two acidophilic, psychrotolerant members of the alphaproteobacteria from acidic northern wetlands. | three obligately aerobic, heterotrophic bacteria, designated strains wm1t, tpb606t and tpb621, were isolated from acidic sphagnum-dominated tundra and siberian wetlands in russia. cells of these isolates were gram-negative, non-motile coccobacilli that occurred singly, in pairs or in chains, and were covered by large capsules. the novel strains were moderately acidophilic and psychrotolerant organisms capable of growth at ph 3.0-7.6 and 2-30 degrees c. cells contained numerous intracellular poly ... | 2009 | 19620354 |
| identification of the metabolically active members of a bacterial community in a polychlorinated biphenyl-polluted moorland soil. | the presumptive metabolically active members of a bacterial community in a moorland soil in germany, highly polluted with polychlorinated biphenyls (pcbs), were identified by sequencing of cloned reverse transcription-polymerase chain reaction (rt-pcr) amplification products of 16s rrna generated from total rna extracts. analysis of the 16s rrna clone library revealed a considerable diversity of metabolically active bacteria in the soil, despite the acidic ph and high concentrations of pcbs. clo ... | 1999 | 11207739 |
| the amino acid sequence of the cytochrome c2 from the phototrophic bacterium rhodopseudomonas globiformis. | the amino acid sequence of the principal soluble cytochrome c from the phototrophic acidophilic bacterium rhodopseudomonas (or rhodopila) globiformis was determined. by the criteria of percentage sequence identity and fewness of internal insertions and deletions it is more similar in sequence to some mitochondrial cytochromes c than to any known bacterial cytochrome. the organism does not have any properties that commend it as being particularly similar to postulated prokaryotic precursors of th ... | 1987 | 2823792 |
| microbial diversity in hydrothermal surface to subsurface environments of suiyo seamount, izu-bonin arc, using a catheter-type in situ growth chamber. | after excavation using a portable submarine driller near deep-sea hydrothermal vents in the suiyo seamount, izu-bonin arc, microbial diversity was examined in samples collected from inside the boreholes using an in situ growth chamber called a vent catheter. this instrument, which we devised for this study, consists of a heat-tolerant pipe tipped with a titanium mesh entrapment capsule that is packed with sterilized inorganic porous grains, which serve as an adhesion substrate. after this instru ... | 2004 | 19712321 |